| 4 |
0.0001192 |
0.08823 |
bsu12150 |
yjgB |
-21.60 |
0.001260 |
37 -- 72 |
32 -- 79 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 1580552 |
|
_____________36_______________________________________________73\n_____________|________________________________________________|\n5'-CCU...UGUGA____UU_____C____________________C_______________GCUUU...CAG-3'\n______________UUGU__UCUUG_CAG__________CCAUCUC__AUUCCCC_UUUCUU\n______________|||:__|||||_||:__________|||||||__|||||||_||||||\n______________AACG__AGAAC_GUU__________GGUAGAG__UAAGGGG_AAAGAA\n3'-AUU...GAAGC____UU_____C___UGUUAGCAUG_______AA_______U______ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________80_______________________________________________31\n |
60 -- 66 |
39 -- 45 |
-34.5565 kcal/mol |
2.03428 kcal/mol |
13.9015 kcal/mol |
36 73@LB@ | |@LB@5'-CCU...UGUGA UU C C GCUUU...CAG-3'@LB@ UUGU UCUUG CAG CCAUCUC AUUCCCC UUUCUU@LB@ |||: ||||| ||: ||||||| ||||||| ||||||@LB@ AACG AGAAC GUU GGUAGAG UAAGGGG AAAGAA@LB@3'-AUU...GAAGC UU C UGUUAGCAUG AA U ACUUU...CAA-5'@LB@ | |@LB@ 80 31@LB@@LB@@LB@Energy -21.60 kcal/mol@LB@Hybridization Energy -34.5565 kcal/mol@LB@Unfolding Energy - mRNA 2.03428 kcal/mol@LB@Unfolding Energy - sRNA 13.9015 kcal/mol@LB@@LB@Position - mRNA 37 -- 72@LB@Position - sRNA 32 -- 79@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 179 |
0.008166 |
0.1751 |
bsu40550 |
ppaC |
-16.23 |
0.018315 |
37 -- 106 |
8 -- 74 |
manganese-dependent inorganic pyrophosphatase |
|
_____________36_______________________________________________________________________107\n_____________|________________________________________________________________________|\n5'-GAC...AUUAC__________C____________GUA_____CACA__U_____CCUUAGCUCAAA__________CUUA___ACAUC...AUG-3'\n______________AUUUU_GCAA_UAAUC_UACCCA___UUUUA____CC_UUUCU____________GAAAAUGCUU____UUC\n______________||:||_||||_:||||_||||||___|:|||____||_|||||____________|||||:||||____|||\n______________UAGAA_CGUU_GUUAG_AUGGGU___AGAAU____GG_AAAGA____________CUUUUGCGAA____AAG\n3'-AUU...AACGU_____C____U_____C______AG______AAGG__U_____AACUUUAAA_____________UUA____AAAACAA-5'\n_____________|________________________________________________________________________|\n_____________75_______________________________________________________________________7\n |
93 -- 99 |
14 -- 20 |
-36.8068 kcal/mol |
5.4197 kcal/mol |
15.7808 kcal/mol |
36 107@LB@ | |@LB@5'-GAC...AUUAC C GUA CACA U CCUUAGCUCAAA CUUA ACAUC...AUG-3'@LB@ AUUUU GCAA UAAUC UACCCA UUUUA CC UUUCU GAAAAUGCUU UUC@LB@ ||:|| |||| :|||| |||||| |:||| || ||||| |||||:|||| |||@LB@ UAGAA CGUU GUUAG AUGGGU AGAAU GG AAAGA CUUUUGCGAA AAG@LB@3'-AUU...AACGU C U C AG AAGG U AACUUUAAA UUA AAAACAA-5'@LB@ | |@LB@ 75 7@LB@@LB@@LB@Energy -16.23 kcal/mol@LB@Hybridization Energy -36.8068 kcal/mol@LB@Unfolding Energy - mRNA 5.4197 kcal/mol@LB@Unfolding Energy - sRNA 15.7808 kcal/mol@LB@@LB@Position - mRNA 37 -- 106@LB@Position - sRNA 8 -- 74@LB@Position Seed - mRNA 93 -- 99@LB@Position Seed - sRNA 14 -- 20@LB@ |
| 103 |
0.004238 |
0.1594 |
bsu09690 |
nhaX |
-18.33 |
0.006526 |
38 -- 80 |
34 -- 77 |
stress response protein NhaX |
|
_____________37________________________________________________81\n_____________|_________________________________________________|\n5'-GGU...AACAC____AUUC_______U____________________CCA____G_____AUCUU...CCG-3'\n______________GCAA____CUUGGCA_AGCAA____ACCC_UC_CUU___UUCU_AUUUC\n______________||||____|||||||_|:|||____||||_||_|||___|:|:_|||||\n______________CGUU____GAACCGU_UUGUU____UGGG_AG_GAA___AGGG_UAAAG\n3'-AUU...AGCAA____A________________AGCA____U__A___UA_____G_____AAACU...CAA-5'\n_____________|_________________________________________________|\n_____________78________________________________________________33\n |
46 -- 52 |
66 -- 72 |
-32.0538 kcal/mol |
3.14542 kcal/mol |
13.7939 kcal/mol |
37 81@LB@ | |@LB@5'-GGU...AACAC AUUC U CCA G AUCUU...CCG-3'@LB@ GCAA CUUGGCA AGCAA ACCC UC CUU UUCU AUUUC@LB@ |||| ||||||| |:||| |||| || ||| |:|: |||||@LB@ CGUU GAACCGU UUGUU UGGG AG GAA AGGG UAAAG@LB@3'-AUU...AGCAA A AGCA U A UA G AAACU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -18.33 kcal/mol@LB@Hybridization Energy -32.0538 kcal/mol@LB@Unfolding Energy - mRNA 3.14542 kcal/mol@LB@Unfolding Energy - sRNA 13.7939 kcal/mol@LB@@LB@Position - mRNA 38 -- 80@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 184 |
0.008461 |
0.1751 |
bsu09120 |
dgcK |
-13.04 |
0.083992 |
38 -- 184 |
37 -- 168 |
diguanylate cyclase |
|
_____________37______________________________________185\n_____________|_______________________________________|\n5'-CAU...AAGUC___AA_______AAACUCUCCCUUCAA____________UACUA...GCA-3'\n______________UCC__AGCAGGU________________AUUUUCUCAUU\n______________|||__||||:||________________|:||||||||:\n______________AGG__UCGUUCA________________UGAAAGAGUAG\n3'-AUU...GUAAC___CG_______AGGUAUAUUACAACAG___________AAGUU...CAA-5'\n_____________|_______________________________________|\n_____________169_____________________________________36\n |
55 -- 61 |
102 -- 108 |
-22.9941 kcal/mol |
2.75938 kcal/mol |
10.7301 kcal/mol |
37 185@LB@ | |@LB@5'-CAU...AAGUC AA AAACUCUCCCUUCAA UACUA...GCA-3'@LB@ UCC AGCAGGU AUUUUCUCAUU@LB@ ||| ||||:|| |:||||||||:@LB@ AGG UCGUUCA UGAAAGAGUAG@LB@3'-AUU...GUAAC CG AGGUAUAUUACAACAG AAGUU...CAA-5'@LB@ | |@LB@ 169 36@LB@@LB@@LB@Energy -13.04 kcal/mol@LB@Hybridization Energy -22.9941 kcal/mol@LB@Unfolding Energy - mRNA 2.75938 kcal/mol@LB@Unfolding Energy - sRNA 10.7301 kcal/mol@LB@@LB@Position - mRNA 38 -- 184@LB@Position - sRNA 37 -- 168@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 127 |
0.005895 |
0.1741 |
bsu15640 |
yloA |
-14.75 |
0.037563 |
39 -- 86 |
36 -- 83 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 11238985 17586671; Product type pf: factor' |
|
_____________38__________________________________________________87\n_____________|___________________________________________________|\n5'-UUA...ACGUA__G_____C____A_______CUGU_____________AUGAAA_______CCGCU...AUA-3'\n______________UU_GUUGC__UCU__GUAAAU____UUGU_CCCAUUUC______CUUCAUU\n______________||_|||||__|||__|:|||:____|:||_|||||:||______|::||||\n______________AA_CAACG__AGA__CGUUUG____AGCA_GGGUAGAG______GGGGUAA\n3'-AUU...UACCG__G_____UU___AC______UU______U________AAUAA________AGAAA...CAA-5'\n_____________|___________________________________________________|\n_____________84__________________________________________________35\n |
80 -- 86 |
36 -- 42 |
-30.597 kcal/mol |
5.41607 kcal/mol |
13.7079 kcal/mol |
38 87@LB@ | |@LB@5'-UUA...ACGUA G C A CUGU AUGAAA CCGCU...AUA-3'@LB@ UU GUUGC UCU GUAAAU UUGU CCCAUUUC CUUCAUU@LB@ || ||||| ||| |:|||: |:|| |||||:|| |::||||@LB@ AA CAACG AGA CGUUUG AGCA GGGUAGAG GGGGUAA@LB@3'-AUU...UACCG G UU AC UU U AAUAA AGAAA...CAA-5'@LB@ | |@LB@ 84 35@LB@@LB@@LB@Energy -14.75 kcal/mol@LB@Hybridization Energy -30.597 kcal/mol@LB@Unfolding Energy - mRNA 5.41607 kcal/mol@LB@Unfolding Energy - sRNA 13.7079 kcal/mol@LB@@LB@Position - mRNA 39 -- 86@LB@Position - sRNA 36 -- 83@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 40 |
0.001293 |
0.1206 |
bsu13810 |
ykvS |
-11.02 |
0.206841 |
41 -- 57 |
31 -- 45 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________40________________58\n_____________|_________________|\n5'-UGU...CAAAA_______CCU_______CUAUU...CGA-3'\n______________AUUCCCC___UUUCUUU\n______________|||||||___|||||||\n______________UAAGGGG___AAAGAAA\n3'-AUU...GAGAA_______U_________CUUUA...CAA-5'\n_____________|_________________|\n_____________46________________30\n |
51 -- 57 |
31 -- 37 |
-15.5048 kcal/mol |
1.25691 kcal/mol |
5.1304 kcal/mol |
40 58@LB@ | |@LB@5'-UGU...CAAAA CCU CUAUU...CGA-3'@LB@ AUUCCCC UUUCUUU@LB@ ||||||| |||||||@LB@ UAAGGGG AAAGAAA@LB@3'-AUU...GAGAA U CUUUA...CAA-5'@LB@ | |@LB@ 46 30@LB@@LB@@LB@Energy -11.02 kcal/mol@LB@Hybridization Energy -15.5048 kcal/mol@LB@Unfolding Energy - mRNA 1.25691 kcal/mol@LB@Unfolding Energy - sRNA 5.1304 kcal/mol@LB@@LB@Position - mRNA 41 -- 57@LB@Position - sRNA 31 -- 45@LB@Position Seed - mRNA 51 -- 57@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 63 |
0.001974 |
0.1214 |
bsu34640 |
yvdD |
-9.92 |
0.323853 |
41 -- 65 |
33 -- 60 |
LOG family protein YvdD |
|
_____________40___________________________66\n_____________|____________________________|\n5'-GAC...GUAAA______UCAUCCAUUUUUCUG_______CAGCU...GAA-3'\n______________ACUCUU_______________CAUCAGC\n______________||||||_______________|||||:|\n______________UGAGAA_______________GUAGUUG\n3'-AUU...AAAAA____________________________UCCUG...CAA-5'\n_____________|____________________________|\n_____________61___________________________32\n |
69 -- 75 |
156 -- 162 |
-11.7297 kcal/mol |
2.56294 kcal/mol |
1.40592 kcal/mol |
40 66@LB@ | |@LB@5'-GAC...GUAAA UCAUCCAUUUUUCUG CAGCU...GAA-3'@LB@ ACUCUU CAUCAGC@LB@ |||||| |||||:|@LB@ UGAGAA GUAGUUG@LB@3'-AUU...AAAAA UCCUG...CAA-5'@LB@ | |@LB@ 61 32@LB@@LB@@LB@Energy -9.92 kcal/mol@LB@Hybridization Energy -11.7297 kcal/mol@LB@Unfolding Energy - mRNA 2.56294 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 41 -- 65@LB@Position - sRNA 33 -- 60@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 53 |
0.001762 |
0.1212 |
bsu10040 |
ecsA |
-7.56 |
0.695140 |
42 -- 51 |
33 -- 42 |
ABC transporter ATP-binding protein |
|
_____________41_________52\n_____________|__________|\n5'-UAU...AAACA__________CAGUU...GCU-3'\n______________CCUCAUUUUU\n______________||:|||||:|\n______________GGGGUAAAGA\n3'-AUU...AAUAA__________AACUU...CAA-5'\n_____________|__________|\n_____________43_________32\n |
45 -- 51 |
33 -- 39 |
-10.6739 kcal/mol |
0.405129 kcal/mol |
4.65815 kcal/mol |
41 52@LB@ | |@LB@5'-UAU...AAACA CAGUU...GCU-3'@LB@ CCUCAUUUUU@LB@ ||:|||||:|@LB@ GGGGUAAAGA@LB@3'-AUU...AAUAA AACUU...CAA-5'@LB@ | |@LB@ 43 32@LB@@LB@@LB@Energy -7.56 kcal/mol@LB@Hybridization Energy -10.6739 kcal/mol@LB@Unfolding Energy - mRNA 0.405129 kcal/mol@LB@Unfolding Energy - sRNA 4.65815 kcal/mol@LB@@LB@Position - mRNA 42 -- 51@LB@Position - sRNA 33 -- 42@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 167 |
0.007775 |
0.1751 |
bsu09889 |
yhzD |
-15.75 |
0.023145 |
43 -- 92 |
39 -- 89 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________42____________________________________________________93\n_____________|_____________________________________________________|\n5'-GGA...CUCCA____U_______AC______AAUU_______C___A____C____C_______UGACC...GUG-3'\n______________AAUG_CUUCGUU__GUGAUC____GUAAGCA__UG_AUCU__CUC___UUCCC\n______________||||_|||||||__|::|||____|:||:||__:|_|:|:__|||___|||||\n______________UUAC_GAAGCAA__CGUUAG____CGUUUGU__GC_UGGG__GAG___AAGGG\n3'-AUU...AUUAC____C_______________AAC________UA__A____UA___AAU_____GUAAA...CAA-5'\n_____________|_____________________________________________________|\n_____________90____________________________________________________38\n |
67 -- 73 |
62 -- 68 |
-30.4166 kcal/mol |
5.34264 kcal/mol |
10.926 kcal/mol |
42 93@LB@ | |@LB@5'-GGA...CUCCA U AC AAUU C A C C UGACC...GUG-3'@LB@ AAUG CUUCGUU GUGAUC GUAAGCA UG AUCU CUC UUCCC@LB@ |||| ||||||| |::||| |:||:|| :| |:|: ||| |||||@LB@ UUAC GAAGCAA CGUUAG CGUUUGU GC UGGG GAG AAGGG@LB@3'-AUU...AUUAC C AAC UA A UA AAU GUAAA...CAA-5'@LB@ | |@LB@ 90 38@LB@@LB@@LB@Energy -15.75 kcal/mol@LB@Hybridization Energy -30.4166 kcal/mol@LB@Unfolding Energy - mRNA 5.34264 kcal/mol@LB@Unfolding Energy - sRNA 10.926 kcal/mol@LB@@LB@Position - mRNA 43 -- 92@LB@Position - sRNA 39 -- 89@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 41 |
0.00131 |
0.1206 |
bsu01580 |
ybaR |
-15.69 |
0.023818 |
44 -- 97 |
34 -- 84 |
sulfate transporter YbaR |
|
_____________43________________________________________________________________98\n_____________|_________________________________________________________________|\n5'-CAG...UUAUU____UACU______A__A___CUC____CCU___U_____A_______A_GU_____________UCUCC...CGA-3'\n______________AUGA____CUUUGU_UG_CAA___CUUG___CAA_ACAAU__UACUCA_C_____UUCCCCGUUU\n______________||||____|||:||_||_|||___||||___|||_|||||__|||:||_|_____||||||:|||\n______________UACU____GAAGCA_AC_GUU___GAAC___GUU_UGUUA__AUGGGU_G_____AAGGGGUAAA\n3'-AUU...UUCAU____UACC_____________A______C___________GC______A_AGAAU__________GAAAC...CAA-5'\n_____________|_________________________________________________________________|\n_____________85________________________________________________________________33\n |
90 -- 96 |
35 -- 41 |
-34.7178 kcal/mol |
7.59764 kcal/mol |
14.4179 kcal/mol |
43 98@LB@ | |@LB@5'-CAG...UUAUU UACU A A CUC CCU U A A GU UCUCC...CGA-3'@LB@ AUGA CUUUGU UG CAA CUUG CAA ACAAU UACUCA C UUCCCCGUUU@LB@ |||| |||:|| || ||| |||| ||| ||||| |||:|| | ||||||:|||@LB@ UACU GAAGCA AC GUU GAAC GUU UGUUA AUGGGU G AAGGGGUAAA@LB@3'-AUU...UUCAU UACC A C GC A AGAAU GAAAC...CAA-5'@LB@ | |@LB@ 85 33@LB@@LB@@LB@Energy -15.69 kcal/mol@LB@Hybridization Energy -34.7178 kcal/mol@LB@Unfolding Energy - mRNA 7.59764 kcal/mol@LB@Unfolding Energy - sRNA 14.4179 kcal/mol@LB@@LB@Position - mRNA 44 -- 97@LB@Position - sRNA 34 -- 84@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 35 -- 41@LB@ |
