| 191 |
0.008727 |
0.1751 |
bsu31280 |
yugU |
-11.72 |
0.152832 |
2 -- 31 |
120 -- 152 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________1___________________________________32\n_____________|___________________________________|\n__________5'-A_______AA__GA______________________CAUCA...GCG-3'\n______________AUAUUGA__CA__G_CCGCA_CAAG___CAUAUGA\n______________|:|||||__||__|_|||||_||||___|||||:|\n______________UGUAACU__GU__C_GGCGU_GUUC___GUAUAUU\n3'-AUU...UGUCC___________AA_A_____C____AAG_______ACAAC...CAA-5'\n_____________|___________________________________|\n_____________153_________________________________119\n |
25 -- 31 |
120 -- 126 |
-21.956 kcal/mol |
3.20784 kcal/mol |
9.17624 kcal/mol |
1 32@LB@ | |@LB@ 5'-A AA GA CAUCA...GCG-3'@LB@ AUAUUGA CA G CCGCA CAAG CAUAUGA@LB@ |:||||| || | ||||| |||| |||||:|@LB@ UGUAACU GU C GGCGU GUUC GUAUAUU@LB@3'-AUU...UGUCC AA A C AAG ACAAC...CAA-5'@LB@ | |@LB@ 153 119@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -21.956 kcal/mol@LB@Unfolding Energy - mRNA 3.20784 kcal/mol@LB@Unfolding Energy - sRNA 9.17624 kcal/mol@LB@@LB@Position - mRNA 2 -- 31@LB@Position - sRNA 120 -- 152@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 8 |
0.0001871 |
0.08823 |
bsu22860 |
yphA |
-19.72 |
0.003251 |
3 -- 123 |
37 -- 178 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________2_______________________________________________________________________________________________________124\n_____________|_______________________________________________________________________________________________________|\n5'-UCG...AAGGC________CCG______________________AC_G__C_______GAUUC______________AAGCUUUGAGUUUUU________CUGUCUU_______GAUCA...UUA-3'\n______________UUGUCGCU___UCA_________UAGUGAAUGG__U_GU_GCGAUCU_____GAC__UC_UGCCUG_______________UCUCUUGU_______UUCCCAU\n______________|||||:||___|||_________||:|||||||__|_||_||:||||_____:||__||_|:||::_______________||||||:|_______|:|||||\n______________AACAGUGA___AGU_________AUUACUUACC__A_CA_CGUUAGA_____UUG__AG_AUGGGU_______________AGAGAAUA_______AGGGGUA\n3'-AUU...AUUAC________AAG___AGAAGUUUC__________GA_G__A_______ACCGU___UU__C___________________________________________AAGAA...CAA-5'\n_____________|_______________________________________________________________________________________________________|\n_____________179_____________________________________________________________________________________________________36\n |
117 -- 123 |
37 -- 43 |
-53.1639 kcal/mol |
19.1133 kcal/mol |
22.4377 kcal/mol |
2 124@LB@ | |@LB@5'-UCG...AAGGC CCG AC G C GAUUC AAGCUUUGAGUUUUU CUGUCUU GAUCA...UUA-3'@LB@ UUGUCGCU UCA UAGUGAAUGG U GU GCGAUCU GAC UC UGCCUG UCUCUUGU UUCCCAU@LB@ |||||:|| ||| ||:||||||| | || ||:|||| :|| || |:||:: ||||||:| |:|||||@LB@ AACAGUGA AGU AUUACUUACC A CA CGUUAGA UUG AG AUGGGU AGAGAAUA AGGGGUA@LB@3'-AUU...AUUAC AAG AGAAGUUUC GA G A ACCGU UU C AAGAA...CAA-5'@LB@ | |@LB@ 179 36@LB@@LB@@LB@Energy -19.72 kcal/mol@LB@Hybridization Energy -53.1639 kcal/mol@LB@Unfolding Energy - mRNA 19.1133 kcal/mol@LB@Unfolding Energy - sRNA 22.4377 kcal/mol@LB@@LB@Position - mRNA 3 -- 123@LB@Position - sRNA 37 -- 178@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 9 |
0.0002071 |
0.08823 |
bsu08425 |
mprF |
-16.98 |
0.012672 |
3 -- 50 |
34 -- 81 |
phosphatidylglycerol lysyltransferase |
|
_____________2_______________________________________________51\n_____________|_______________________________________________|\n_________5'-AC__________GU_______U__AU__________AUCAC________CAUUC...AUC-3'\n______________UGUUGU_AUC___GUAAAU_AU____UCCAUUUC_____UUCCUCAU\n______________:||||:_|||___|:|||:_||____:||||:||_____||||:|||\n______________GCAACG_UAG___CGUUUG_UA____GGGUAGAG_____AAGGGGUA\n3'-AUU...CCGAA______U___AAC______U__GCAU________AAU__________AAGAA...CAA-5'\n_____________|_______________________________________________|\n_____________82______________________________________________33\n |
39 -- 45 |
37 -- 43 |
-29.0114 kcal/mol |
1.80779 kcal/mol |
13.626 kcal/mol |
2 51@LB@ | |@LB@ 5'-AC GU U AU AUCAC CAUUC...AUC-3'@LB@ UGUUGU AUC GUAAAU AU UCCAUUUC UUCCUCAU@LB@ :||||: ||| |:|||: || :||||:|| ||||:|||@LB@ GCAACG UAG CGUUUG UA GGGUAGAG AAGGGGUA@LB@3'-AUU...CCGAA U AAC U GCAU AAU AAGAA...CAA-5'@LB@ | |@LB@ 82 33@LB@@LB@@LB@Energy -16.98 kcal/mol@LB@Hybridization Energy -29.0114 kcal/mol@LB@Unfolding Energy - mRNA 1.80779 kcal/mol@LB@Unfolding Energy - sRNA 13.626 kcal/mol@LB@@LB@Position - mRNA 3 -- 50@LB@Position - sRNA 34 -- 81@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 25 |
0.0006626 |
0.1027 |
bsu07955 |
yfkA |
-14.87 |
0.035327 |
3 -- 70 |
36 -- 108 |
radical SAM protein YfkA |
|
_____________2______________71\n_____________|______________|\n5'-AGU...GCUAC______________AAUCA...ACG-3'\n______________UUGCGAUCUUGGCA\n______________||||:|||||||||\n______________AACGUUAGAACCGU\n3'-AUU...GAAGC______________UUGUU...CAA-5'\n_____________|______________|\n_____________109____________35\n |
130 -- 136 |
66 -- 72 |
-22.3996 kcal/mol |
5.8798 kcal/mol |
4.6863 kcal/mol |
2 71@LB@ | |@LB@5'-AGU...GCUAC AAUCA...ACG-3'@LB@ UUGCGAUCUUGGCA@LB@ ||||:|||||||||@LB@ AACGUUAGAACCGU@LB@3'-AUU...GAAGC UUGUU...CAA-5'@LB@ | |@LB@ 109 35@LB@@LB@@LB@Energy -14.87 kcal/mol@LB@Hybridization Energy -22.3996 kcal/mol@LB@Unfolding Energy - mRNA 5.8798 kcal/mol@LB@Unfolding Energy - sRNA 4.6863 kcal/mol@LB@@LB@Position - mRNA 3 -- 70@LB@Position - sRNA 36 -- 108@LB@Position Seed - mRNA 130 -- 136@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 42 |
0.001312 |
0.1206 |
bsu39060 |
citH |
-14.25 |
0.047679 |
3 -- 65 |
120 -- 184 |
citrate transporter |
bsu26860 bsu07610 |
_____________2___________________________________________________________66\n_____________|___________________________________________________________|\n5'-GUC...UCCCC_______AAACGAAACAU__U____C_____U_______________UUC_________ACUGC...UUG-3'\n______________CUUUUUA___________UC_ACAG_ACAUU_AC_____CCGUGGCG___UUCAUAUAA\n______________||:||:|___________||_||||_|||||_||_____|||::||:___|:|||||||\n______________GAGAAGU___________AG_UGUC_UGUAA_UG_____GGCGUCGU___AGGUAUAUU\n3'-AUU...AAAAU____________________U____C_____C__UAACA________UCA_________ACAAC...CAA-5'\n_____________|___________________________________________________________|\n_____________185_________________________________________________________119\n |
59 -- 65 |
120 -- 126 |
-29.4475 kcal/mol |
5.73383 kcal/mol |
10.5645 kcal/mol |
2 66@LB@ | |@LB@5'-GUC...UCCCC AAACGAAACAU U C U UUC ACUGC...UUG-3'@LB@ CUUUUUA UC ACAG ACAUU AC CCGUGGCG UUCAUAUAA@LB@ ||:||:| || |||| ||||| || |||::||: |:|||||||@LB@ GAGAAGU AG UGUC UGUAA UG GGCGUCGU AGGUAUAUU@LB@3'-AUU...AAAAU U C C UAACA UCA ACAAC...CAA-5'@LB@ | |@LB@ 185 119@LB@@LB@@LB@Energy -14.25 kcal/mol@LB@Hybridization Energy -29.4475 kcal/mol@LB@Unfolding Energy - mRNA 5.73383 kcal/mol@LB@Unfolding Energy - sRNA 10.5645 kcal/mol@LB@@LB@Position - mRNA 3 -- 65@LB@Position - sRNA 120 -- 184@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 100 |
0.004029 |
0.1561 |
bsu32980 |
yusZ |
-14.16 |
0.049671 |
3 -- 55 |
39 -- 90 |
oxidoreductase |
|
_____________2______________________________________56\n_____________|______________________________________|\n5'-CCG...GUCAU_____GUU_______GU____AUAG_____________AAUAG...CAA-3'\n______________UUCGU___CAGGAUA___ACA____CUGUAGCA_GUUU\n______________|||:|___|||||:|___|||____|:|:||||_|||:\n______________AAGUA___GUCCUGU___UGU____GGCGUCGU_CAAG\n3'-AUU...AUGAG_____GUU_______AAC___AACA________U____GUAUA...CAA-5'\n_____________|______________________________________|\n_____________91_____________________________________38\n |
175 -- 181 |
132 -- 138 |
-30.394 kcal/mol |
8.27302 kcal/mol |
10.1352 kcal/mol |
2 56@LB@ | |@LB@5'-CCG...GUCAU GUU GU AUAG AAUAG...CAA-3'@LB@ UUCGU CAGGAUA ACA CUGUAGCA GUUU@LB@ |||:| |||||:| ||| |:|:|||| |||:@LB@ AAGUA GUCCUGU UGU GGCGUCGU CAAG@LB@3'-AUU...AUGAG GUU AAC AACA U GUAUA...CAA-5'@LB@ | |@LB@ 91 38@LB@@LB@@LB@Energy -14.16 kcal/mol@LB@Hybridization Energy -30.394 kcal/mol@LB@Unfolding Energy - mRNA 8.27302 kcal/mol@LB@Unfolding Energy - sRNA 10.1352 kcal/mol@LB@@LB@Position - mRNA 3 -- 55@LB@Position - sRNA 39 -- 90@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 132 -- 138@LB@ |
| 106 |
0.004415 |
0.1614 |
bsu10790 |
asnO |
-17.17 |
0.011578 |
3 -- 52 |
32 -- 73 |
asparagine synthetase 3 |
|
_____________2__________________________________________________53\n_____________|__________________________________________________|\n_________5'-CC___GUAUAU_________U________AUCCAUGCUG_____UU______CAUUC...CUG-3'\n______________UCU______AACAAUUGU_UCCGUUUC__________UCUCC__UUUCUU\n______________|||______||||||:||_:||:|:||__________||:||__||||||\n______________AGA______UUGUUAGCA_GGGUAGAG__________AGGGG__AAAGAA\n3'-AUU...ACGUU___ACCGU__________U________AAUA___________U_______ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________74_________________________________________________31\n |
23 -- 29 |
48 -- 54 |
-28.5385 kcal/mol |
1.12136 kcal/mol |
12.9474 kcal/mol |
2 53@LB@ | |@LB@ 5'-CC GUAUAU U AUCCAUGCUG UU CAUUC...CUG-3'@LB@ UCU AACAAUUGU UCCGUUUC UCUCC UUUCUU@LB@ ||| ||||||:|| :||:|:|| ||:|| ||||||@LB@ AGA UUGUUAGCA GGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACGUU ACCGU U AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 74 31@LB@@LB@@LB@Energy -17.17 kcal/mol@LB@Hybridization Energy -28.5385 kcal/mol@LB@Unfolding Energy - mRNA 1.12136 kcal/mol@LB@Unfolding Energy - sRNA 12.9474 kcal/mol@LB@@LB@Position - mRNA 3 -- 52@LB@Position - sRNA 32 -- 73@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 197 |
0.009002 |
0.1751 |
bsu33650 |
yvzC |
-16.51 |
0.015985 |
3 -- 52 |
121 -- 172 |
HTH-type transcriptional regulator YvzC |
|
_____________2__________________________________________________53\n_____________|__________________________________________________|\n____5'-ACUUUCU_______C_____AU__CC__UA_________CAA___U___________UCUCA...UCU-3'\n______________CUCUUCA___GCA__GC___G__AUUGUUCGC___AGC___UUCCAUAUA\n______________|||||||___:||__:|___|__|||||:|||___|||___|||||||||\n______________GAGAAGU___UGU__UG___C__UAACAGGCG___UCG___AAGGUAUAU\n3'-AUU...AAAAU_______AGU___CC__UAA_UG_______________UUC_________UACAA...CAA-5'\n_____________|__________________________________________________|\n_____________173________________________________________________120\n |
46 -- 52 |
121 -- 127 |
-33.6584 kcal/mol |
6.173 kcal/mol |
10.5345 kcal/mol |
2 53@LB@ | |@LB@ 5'-ACUUUCU C AU CC UA CAA U UCUCA...UCU-3'@LB@ CUCUUCA GCA GC G AUUGUUCGC AGC UUCCAUAUA@LB@ ||||||| :|| :| | |||||:||| ||| |||||||||@LB@ GAGAAGU UGU UG C UAACAGGCG UCG AAGGUAUAU@LB@3'-AUU...AAAAU AGU CC UAA UG UUC UACAA...CAA-5'@LB@ | |@LB@ 173 120@LB@@LB@@LB@Energy -16.51 kcal/mol@LB@Hybridization Energy -33.6584 kcal/mol@LB@Unfolding Energy - mRNA 6.173 kcal/mol@LB@Unfolding Energy - sRNA 10.5345 kcal/mol@LB@@LB@Position - mRNA 3 -- 52@LB@Position - sRNA 121 -- 172@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 64 |
0.002007 |
0.1215 |
bsu37680 |
ywfH |
-19.51 |
0.003623 |
4 -- 58 |
32 -- 83 |
bacilysin biosynthesis oxidoreductase YwfH |
|
_____________3___________________________________________________________59\n_____________|___________________________________________________________|\n_________5'-AA___G______U____U__C_____________U__G____AUACAC_______A_____AUAUG...AUU-3'\n______________UUU_GUU_CA_UCUU_GU_GGCAAUC__ACCU_UC_CUUG______UUCCUCA_UUCUU\n______________:||_|||_||_||||_|:_::|||||__|||:_||_|||:______||||:||_|||||\n______________GAA_CAA_GU_AGAA_CG_UUGUUAG__UGGG_AG_GAAU______AAGGGGU_AAGAA\n3'-AUU...UUACC___G___C__U____C__U_______CA____U__A_________________A_____ACUUU...CAA-5'\n_____________|___________________________________________________________|\n_____________84__________________________________________________________31\n |
46 -- 52 |
38 -- 44 |
-36.3278 kcal/mol |
5.00359 kcal/mol |
14.596 kcal/mol |
3 59@LB@ | |@LB@ 5'-AA G U U C U G AUACAC A AUAUG...AUU-3'@LB@ UUU GUU CA UCUU GU GGCAAUC ACCU UC CUUG UUCCUCA UUCUU@LB@ :|| ||| || |||| |: ::||||| |||: || |||: ||||:|| |||||@LB@ GAA CAA GU AGAA CG UUGUUAG UGGG AG GAAU AAGGGGU AAGAA@LB@3'-AUU...UUACC G C U C U CA U A A ACUUU...CAA-5'@LB@ | |@LB@ 84 31@LB@@LB@@LB@Energy -19.51 kcal/mol@LB@Hybridization Energy -36.3278 kcal/mol@LB@Unfolding Energy - mRNA 5.00359 kcal/mol@LB@Unfolding Energy - sRNA 14.596 kcal/mol@LB@@LB@Position - mRNA 4 -- 58@LB@Position - sRNA 32 -- 83@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 69 |
0.002314 |
0.1242 |
bsu15650 |
yloB |
-14.32 |
0.046100 |
4 -- 34 |
102 -- 149 |
calcium-transporting ATPase |
|
_____________3________________________________________35\n_____________|________________________________________|\n5'-UUU...AAUUC________________________________________AAGUU...ACA-3'\n______________GUCCGC________CCA__________UUAUUUUCUCGUU\n______________||||||________|||__________|:|:||||||:|:\n______________CAGGCG________GGU__________AGUGAAAGAGUAG\n3'-AUU...UGUAA______UCGUUCAA___AUAUUACAAC_____________AAGUU...CAA-5'\n_____________|________________________________________|\n_____________150______________________________________101\n |
28 -- 34 |
102 -- 108 |
-22.6804 kcal/mol |
0.390781 kcal/mol |
10.7571 kcal/mol |
3 35@LB@ | |@LB@5'-UUU...AAUUC AAGUU...ACA-3'@LB@ GUCCGC CCA UUAUUUUCUCGUU@LB@ |||||| ||| |:|:||||||:|:@LB@ CAGGCG GGU AGUGAAAGAGUAG@LB@3'-AUU...UGUAA UCGUUCAA AUAUUACAAC AAGUU...CAA-5'@LB@ | |@LB@ 150 101@LB@@LB@@LB@Energy -14.32 kcal/mol@LB@Hybridization Energy -22.6804 kcal/mol@LB@Unfolding Energy - mRNA 0.390781 kcal/mol@LB@Unfolding Energy - sRNA 10.7571 kcal/mol@LB@@LB@Position - mRNA 4 -- 34@LB@Position - sRNA 102 -- 149@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 102 -- 108@LB@ |
