| 83 |
0.002931 |
0.1368 |
bsu18650 |
pelB |
-17.38 |
0.010445 |
17 -- 103 |
38 -- 142 |
pectin lyase |
|
_____________16________________________________________________________________________________________________________________104\n_____________|_________________________________________________________________________________________________________________|\n5'-AGG...UAAGC_________U_______G_____UCAA____________CUA_________________________A___U____UU__________________________AGUAA____ACAUU...CAA-3'\n______________UG_CUGCAG_CAA___C_GUGUA______UC_CUUUCUU___UUUCAA______________UUGUU_GUA_GUCU__UGGC__GCAAUC___UCUAUUUUUUA_____CCCA\n______________||_|:||||_|||___|_:|:||______||_||||||:___:|||||______________|:|||_|:|_:|||__||||__:|||||___:|:||:|:|||_____||||\n______________AC_GGCGUC_GUU___G_UAUAU______AG_GAAAGAG___GAAGUU______________AGCAA_CGU_UAGA__ACCG__UGUUAG___GGGUAGAGAAU_____GGGU\n3'-AUU...CUGUA__A__________CAA_G_____UACAAC__U_______UA_______UCAUUACUUACCGA____________________UU______CAU___________AAG______AAAGA...CAA-5'\n_____________|_________________________________________________________________________________________________________________|\n_____________143_______________________________________________________________________________________________________________37\n |
42 -- 48 |
105 -- 111 |
-52.303 kcal/mol |
11.0023 kcal/mol |
27.6117 kcal/mol |
16 104@LB@ | |@LB@5'-AGG...UAAGC U G UCAA CUA A U UU AGUAA ACAUU...CAA-3'@LB@ UG CUGCAG CAA C GUGUA UC CUUUCUU UUUCAA UUGUU GUA GUCU UGGC GCAAUC UCUAUUUUUUA CCCA@LB@ || |:|||| ||| | :|:|| || ||||||: :||||| |:||| |:| :||| |||| :||||| :|:||:|:||| ||||@LB@ AC GGCGUC GUU G UAUAU AG GAAAGAG GAAGUU AGCAA CGU UAGA ACCG UGUUAG GGGUAGAGAAU GGGU@LB@3'-AUU...CUGUA A CAA G UACAAC U UA UCAUUACUUACCGA UU CAU AAG AAAGA...CAA-5'@LB@ | |@LB@ 143 37@LB@@LB@@LB@Energy -17.38 kcal/mol@LB@Hybridization Energy -52.303 kcal/mol@LB@Unfolding Energy - mRNA 11.0023 kcal/mol@LB@Unfolding Energy - sRNA 27.6117 kcal/mol@LB@@LB@Position - mRNA 17 -- 103@LB@Position - sRNA 38 -- 142@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 94 |
0.003675 |
0.1515 |
bsu40130 |
yydK |
-17.16 |
0.011624 |
17 -- 47 |
34 -- 60 |
HTH-type transcriptional regulator YydK |
|
_____________16___________________________________48\n_____________|____________________________________|\n__________5'-A_____UUU__U___C_________CUC_________CCCGA...UGG-3'\n______________UGUCU___GC_AGU_UCGUAUAAU____UCACUUUC\n______________||||:___||_|||_:|:||||||____||||||||\n______________ACAGG___CG_UCA_GGUAUAUUA____AGUGAAAG\n3'-AUU...CUGUA_____CGU__U___A_________CAAC________AGUAG...CAA-5'\n_____________|____________________________________|\n_____________61___________________________________33\n |
30 -- 36 |
107 -- 113 |
-26.8798 kcal/mol |
2.26958 kcal/mol |
10.0128 kcal/mol |
16 48@LB@ | |@LB@ 5'-A UUU U C CUC CCCGA...UGG-3'@LB@ UGUCU GC AGU UCGUAUAAU UCACUUUC@LB@ ||||: || ||| :|:|||||| ||||||||@LB@ ACAGG CG UCA GGUAUAUUA AGUGAAAG@LB@3'-AUU...CUGUA CGU U A CAAC AGUAG...CAA-5'@LB@ | |@LB@ 61 33@LB@@LB@@LB@Energy -17.16 kcal/mol@LB@Hybridization Energy -26.8798 kcal/mol@LB@Unfolding Energy - mRNA 2.26958 kcal/mol@LB@Unfolding Energy - sRNA 10.0128 kcal/mol@LB@@LB@Position - mRNA 17 -- 47@LB@Position - sRNA 34 -- 60@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 43 |
0.00135 |
0.1206 |
bsu10230 |
yhfH |
-10.18 |
0.291514 |
18 -- 110 |
27 -- 139 |
Evidence 6: Doubtful CDS |
|
_____________17_____________________________________________________________________________________________________________________111\n_____________|______________________________________________________________________________________________________________________|\n5'-CAU...GAAAA____CAU________________C_____A______A________________UCCUC_______________UU____AA_____GU___CC___AAUAUA_________C______AAAGC...AUG-3'\n______________CCGC___AGCA___UCCG_AUAA___UGU_ACUUUC____________UAAUG_____CU________AUCUU__CAAA__AAUUG__CCU__UUC______CCCAUUUUU_UUGAAA\n______________||||___||||___|||:_||||___|||_||||||____________|||||_____||________|||||__||||__|||:|__||:__:||______|||||||:|_||||||\n______________GGCG___UCGU___AGGU_UAUU___ACA_UGAAAG____________AUUAC_____GA________UAGAA__GUUU__UUAGC__GGG__GAG______GGGUAAAGA_AACUUU\n3'-AUU...UAACA___________UCA____A____ACA___G______AGUAGAAGUUUC_____UUACC__AGCAACGU_____CC____G______AU___UA___AAUAAG________________AAACU...CAA-5'\n_____________|______________________________________________________________________________________________________________________|\n_____________140____________________________________________________________________________________________________________________26\n |
96 -- 102 |
34 -- 40 |
-47.8615 kcal/mol |
11.539 kcal/mol |
28.2686 kcal/mol |
17 111@LB@ | |@LB@5'-CAU...GAAAA CAU C A A UCCUC UU AA GU CC AAUAUA C AAAGC...AUG-3'@LB@ CCGC AGCA UCCG AUAA UGU ACUUUC UAAUG CU AUCUU CAAA AAUUG CCU UUC CCCAUUUUU UUGAAA@LB@ |||| |||| |||: |||| ||| |||||| ||||| || ||||| |||| |||:| ||: :|| |||||||:| ||||||@LB@ GGCG UCGU AGGU UAUU ACA UGAAAG AUUAC GA UAGAA GUUU UUAGC GGG GAG GGGUAAAGA AACUUU@LB@3'-AUU...UAACA UCA A ACA G AGUAGAAGUUUC UUACC AGCAACGU CC G AU UA AAUAAG AAACU...CAA-5'@LB@ | |@LB@ 140 26@LB@@LB@@LB@Energy -10.18 kcal/mol@LB@Hybridization Energy -47.8615 kcal/mol@LB@Unfolding Energy - mRNA 11.539 kcal/mol@LB@Unfolding Energy - sRNA 28.2686 kcal/mol@LB@@LB@Position - mRNA 18 -- 110@LB@Position - sRNA 27 -- 139@LB@Position Seed - mRNA 96 -- 102@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 123 |
0.005649 |
0.1741 |
bsu37530 |
ywhC |
-10.56 |
0.250674 |
18 -- 29 |
105 -- 116 |
zinc metalloprotease YwhC |
|
_____________17___________30\n_____________|____________|\n5'-GUU...GCUUC____________GACAA...UCG-3'\n______________UGUUACUUUCUC\n______________|||:||||||||\n______________ACAGUGAAAGAG\n3'-AUU...UUACA____________UAGAA...CAA-5'\n_____________|____________|\n_____________117__________104\n |
23 -- 29 |
105 -- 111 |
-16.5612 kcal/mol |
1.98074 kcal/mol |
5.11978 kcal/mol |
17 30@LB@ | |@LB@5'-GUU...GCUUC GACAA...UCG-3'@LB@ UGUUACUUUCUC@LB@ |||:||||||||@LB@ ACAGUGAAAGAG@LB@3'-AUU...UUACA UAGAA...CAA-5'@LB@ | |@LB@ 117 104@LB@@LB@@LB@Energy -10.56 kcal/mol@LB@Hybridization Energy -16.5612 kcal/mol@LB@Unfolding Energy - mRNA 1.98074 kcal/mol@LB@Unfolding Energy - sRNA 5.11978 kcal/mol@LB@@LB@Position - mRNA 18 -- 29@LB@Position - sRNA 105 -- 116@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 130 |
0.005995 |
0.1741 |
bsu19660 |
yozD |
-10.24 |
0.285413 |
18 -- 51 |
105 -- 135 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________17_________________________________52\n_____________|__________________________________|\n5'-CAC...CUUUG____UUUU_____U______AUCAAAU_______CGUCC...UGU-3'\n______________AGCG____UUCCA_UAUGAU_______CUUUCUC\n______________|||:____|||||_|||:||_______|||||||\n______________UCGU____AAGGU_AUAUUA_______GAAAGAG\n3'-AUU...AGGCG____UC______________CAACAGU_______UAGAA...CAA-5'\n_____________|__________________________________|\n_____________136________________________________104\n |
45 -- 51 |
105 -- 111 |
-19.2876 kcal/mol |
2.67508 kcal/mol |
6.61557 kcal/mol |
17 52@LB@ | |@LB@5'-CAC...CUUUG UUUU U AUCAAAU CGUCC...UGU-3'@LB@ AGCG UUCCA UAUGAU CUUUCUC@LB@ |||: ||||| |||:|| |||||||@LB@ UCGU AAGGU AUAUUA GAAAGAG@LB@3'-AUU...AGGCG UC CAACAGU UAGAA...CAA-5'@LB@ | |@LB@ 136 104@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -19.2876 kcal/mol@LB@Unfolding Energy - mRNA 2.67508 kcal/mol@LB@Unfolding Energy - sRNA 6.61557 kcal/mol@LB@@LB@Position - mRNA 18 -- 51@LB@Position - sRNA 105 -- 135@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 24 |
0.0006259 |
0.1011 |
bsu03380 |
yckB |
-16.82 |
0.013754 |
20 -- 129 |
16 -- 148 |
ABC transporter substrate-binding protein |
|
_____________19_________________________________________________________________________________________________________________________________________________130\n_____________|__________________________________________________________________________________________________________________________________________________|\n5'-UGC...UCCUU__U____C_______CA___U___A________UUUUUUCA_______C_____CC_____U__AC_______________________________CCC________G_____UGUAUA_A__A___GAACUU____________GAUCA...CCA-3'\n______________GG_ACGU_UGGCAUU__UCC_UGG_CAA_UUCC___________UCAC__UCUU__CUUCA_AG_________GCUUU_____________UAAACA_____ACCCAU_CUUUU______C_CA_UUC_______UUUGAAAACGC\n______________||_||:|_||:||||__|||_::|_|||_||||___________||||__|||:__|||||_||_________||||:_____________:|||||_____||||||_||:||______|_||_|||_______|||||||||||\n______________CC_UGUA_ACUGUAA__AGG_GUC_GUU_AAGG___________AGUG__AGAG__GAAGU_UC_________CGAAG_____________GUUUGU_____UGGGUA_GAGAA______G_GU_AAG_______AAACUUUUGCG\n3'-AUU...GUUGU_______________C____C_______C____UAUAUUACAAC____AA____UA_____U__AUUACUUAC_____CAACGUUAGAACC______UAGCA____________UAAGG_____A___AAACUUU___________AAUUA...CAA-5'\n_____________|__________________________________________________________________________________________________________________________________________________|\n_____________149________________________________________________________________________________________________________________________________________________15\n |
123 -- 129 |
16 -- 22 |
-61.2162 kcal/mol |
13.3554 kcal/mol |
33.3199 kcal/mol |
19 130@LB@ | |@LB@5'-UGC...UCCUU U C CA U A UUUUUUCA C CC U AC CCC G UGUAUA A A GAACUU GAUCA...CCA-3'@LB@ GG ACGU UGGCAUU UCC UGG CAA UUCC UCAC UCUU CUUCA AG GCUUU UAAACA ACCCAU CUUUU C CA UUC UUUGAAAACGC@LB@ || ||:| ||:|||| ||| ::| ||| |||| |||| |||: ||||| || ||||: :||||| |||||| ||:|| | || ||| |||||||||||@LB@ CC UGUA ACUGUAA AGG GUC GUU AAGG AGUG AGAG GAAGU UC CGAAG GUUUGU UGGGUA GAGAA G GU AAG AAACUUUUGCG@LB@3'-AUU...GUUGU C C C UAUAUUACAAC AA UA U AUUACUUAC CAACGUUAGAACC UAGCA UAAGG A AAACUUU AAUUA...CAA-5'@LB@ | |@LB@ 149 15@LB@@LB@@LB@Energy -16.82 kcal/mol@LB@Hybridization Energy -61.2162 kcal/mol@LB@Unfolding Energy - mRNA 13.3554 kcal/mol@LB@Unfolding Energy - sRNA 33.3199 kcal/mol@LB@@LB@Position - mRNA 20 -- 129@LB@Position - sRNA 16 -- 148@LB@Position Seed - mRNA 123 -- 129@LB@Position Seed - sRNA 16 -- 22@LB@ |
| 54 |
0.001793 |
0.1212 |
bsu21030 |
yonP |
-11.11 |
0.198660 |
20 -- 36 |
161 -- 178 |
Evidence 5: No homology to any previously reported sequences |
|
_____________19_______37\n_____________|________|\n5'-AAU...UUUAA________AUAUU...ACA-3'\n______________ACUCUUUA\n______________||||||:|\n______________UGAGAAGU\n3'-AUU...AAAAA________AGUUG...CAA-5'\n_____________|________|\n_____________179______160\n |
30 -- 36 |
161 -- 167 |
-8.6372 kcal/mol |
0.252156 kcal/mol |
0.576912 kcal/mol |
19 37@LB@ | |@LB@5'-AAU...UUUAA AUAUU...ACA-3'@LB@ ACUCUUUA@LB@ ||||||:|@LB@ UGAGAAGU@LB@3'-AUU...AAAAA AGUUG...CAA-5'@LB@ | |@LB@ 179 160@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -8.6372 kcal/mol@LB@Unfolding Energy - mRNA 0.252156 kcal/mol@LB@Unfolding Energy - sRNA 0.576912 kcal/mol@LB@@LB@Position - mRNA 20 -- 36@LB@Position - sRNA 161 -- 178@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 161 -- 167@LB@ |
| 145 |
0.006739 |
0.1741 |
bsu12460 |
xlyB |
-11.80 |
0.146979 |
21 -- 64 |
155 -- 186 |
N-acetylmuramoyl-L-alanine amidase XlyB |
bsu12810 bsu25710 bsu25900 bsu21410 |
_____________20____________________________________65\n_____________|_____________________________________|\n5'-UCC...CCACA_____C_G_____AUUUCUC____CU_______A___UUAAA...CGG-3'\n______________UGCUC_U_AUUAA_______CGUU__CAUUGUC_GCA\n______________|:|||_|_||:||_______|:||__|||||||_|||\n______________AUGAG_A_UAGUU_______GUAA__GUAACAG_CGU\n3'-AUU...AAAAA_____A_G_____GUCCU______CU_______G___CGUUC...CAA-5'\n_____________|_____________________________________|\n_____________187___________________________________154\n |
54 -- 60 |
139 -- 145 |
-22.5299 kcal/mol |
4.58402 kcal/mol |
8.54622 kcal/mol |
20 65@LB@ | |@LB@5'-UCC...CCACA C G AUUUCUC CU A UUAAA...CGG-3'@LB@ UGCUC U AUUAA CGUU CAUUGUC GCA@LB@ |:||| | ||:|| |:|| ||||||| |||@LB@ AUGAG A UAGUU GUAA GUAACAG CGU@LB@3'-AUU...AAAAA A G GUCCU CU G CGUUC...CAA-5'@LB@ | |@LB@ 187 154@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -22.5299 kcal/mol@LB@Unfolding Energy - mRNA 4.58402 kcal/mol@LB@Unfolding Energy - sRNA 8.54622 kcal/mol@LB@@LB@Position - mRNA 21 -- 64@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 139 -- 145@LB@ |
| 29 |
0.000814 |
0.1059 |
bsu35860 |
pgdS |
-7.84 |
0.648005 |
23 -- 34 |
130 -- 140 |
gamma-dl-glutamyl hydrolase |
|
______________23________________________________35\n______________|_________________________________|\n___________5'-________GGAAAUGAAUAAAA___A________AAGAA...AUA-3'\n______________AUUGAUAU______________UCC_GCAGCAGG\n______________|||||:||______________|||_||||||:|\n______________UAACUGUA______________AGG_CGUCGUUC\n3'-AUU...UCCUG________AC________________________AAGGU...CAA-5'\n_____________|__________________________________|\n_____________141________________________________129\n |
28 -- 34 |
130 -- 136 |
-20.8842 kcal/mol |
7.79874 kcal/mol |
6.50607 kcal/mol |
23 35@LB@ | |@LB@ 5'- GGAAAUGAAUAAAA A AAGAA...AUA-3'@LB@ AUUGAUAU UCC GCAGCAGG@LB@ |||||:|| ||| ||||||:|@LB@ UAACUGUA AGG CGUCGUUC@LB@3'-AUU...UCCUG AC AAGGU...CAA-5'@LB@ | |@LB@ 141 129@LB@@LB@@LB@Energy -7.84 kcal/mol@LB@Hybridization Energy -20.8842 kcal/mol@LB@Unfolding Energy - mRNA 7.79874 kcal/mol@LB@Unfolding Energy - sRNA 6.50607 kcal/mol@LB@@LB@Position - mRNA 23 -- 34@LB@Position - sRNA 130 -- 140@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 32 |
0.0008744 |
0.1059 |
bsu09430 |
citR |
-15.39 |
0.027619 |
23 -- 103 |
39 -- 111 |
LysR family transcriptional regulator |
|
_____________22______________________________________________________________________________________104\n_____________|_______________________________________________________________________________________|\n5'-UGU...ACGCC________C_A____UAU__C______________UACACA_______CCCUU________CGUAAU__________G_________CUAUC...CCG-3'\n______________CUUUUUCA_C_UCAA___GG_UGAUGGAU_GUUUC______UGUAAUU________UAAUC______GUA_CCAUUU__UAUUCCCC\n______________||||:|||_|_||||___:|_|:|||:||_|:|||______||:|||:________:||||______|||_||||:|__||||||||\n______________GAAAGAGU_G_AGUU___UC_AUUACUUA_CGAAG______ACGUUAG________GUUAG______CAU_GGUAGA__AUAAGGGG\n3'-AUU...ACAGU________A_A__________________C_____CA___________AACCGUUU______________G______GA________UAAAG...CAA-5'\n_____________|_______________________________________________________________________________________|\n_____________112_____________________________________________________________________________________38\n |
97 -- 103 |
39 -- 45 |
-43.1846 kcal/mol |
11.0009 kcal/mol |
20.821 kcal/mol |
22 104@LB@ | |@LB@5'-UGU...ACGCC C A UAU C UACACA CCCUU CGUAAU G CUAUC...CCG-3'@LB@ CUUUUUCA C UCAA GG UGAUGGAU GUUUC UGUAAUU UAAUC GUA CCAUUU UAUUCCCC@LB@ ||||:||| | |||| :| |:|||:|| |:||| ||:|||: :|||| ||| ||||:| ||||||||@LB@ GAAAGAGU G AGUU UC AUUACUUA CGAAG ACGUUAG GUUAG CAU GGUAGA AUAAGGGG@LB@3'-AUU...ACAGU A A C CA AACCGUUU G GA UAAAG...CAA-5'@LB@ | |@LB@ 112 38@LB@@LB@@LB@Energy -15.39 kcal/mol@LB@Hybridization Energy -43.1846 kcal/mol@LB@Unfolding Energy - mRNA 11.0009 kcal/mol@LB@Unfolding Energy - sRNA 20.821 kcal/mol@LB@@LB@Position - mRNA 23 -- 103@LB@Position - sRNA 39 -- 111@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 39 -- 45@LB@ |
