| 45 |
0.001428 |
0.1206 |
bsu27290 |
yrzA |
-10.49 |
0.257926 |
1 -- 17 |
111 -- 136 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________0_________________18\n_____________|_________________|\n_______5'-CGGC_________________ACUCC...ACA-3'\n______________CCAUAU____UUGUCAC\n______________||||||____|||||||\n______________GGUAUA____AACAGUG\n3'-AUU...UUCAA______UUAC_______AAAGA...CAA-5'\n_____________|_________________|\n_____________137_______________110\n |
11 -- 17 |
111 -- 117 |
-14.7924 kcal/mol |
0.950105 kcal/mol |
3.76381 kcal/mol |
0 18@LB@ | |@LB@ 5'-CGGC ACUCC...ACA-3'@LB@ CCAUAU UUGUCAC@LB@ |||||| |||||||@LB@ GGUAUA AACAGUG@LB@3'-AUU...UUCAA UUAC AAAGA...CAA-5'@LB@ | |@LB@ 137 110@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -14.7924 kcal/mol@LB@Unfolding Energy - mRNA 0.950105 kcal/mol@LB@Unfolding Energy - sRNA 3.76381 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 111 -- 136@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 50 |
0.001627 |
0.1212 |
bsu38130 |
ywcE |
-9.91 |
0.324646 |
1 -- 15 |
39 -- 56 |
spore morphogenesis and germination protein YwcE |
|
_____________0_______________________________________________________________16\n_____________|_______________________________________________________________|\n5'-GCC...AAAAA_____ACA______________A__A___A______U____________UU____U_______AUCAC...CAA-3'\n______________CAAUC_____AAACAG____AC_CA_CUC__GUUCC_CUAUUUC____A__UUUG_AAACGUU\n______________|||||_____|||||:____||_||_|||__:||||_|:|||||____|__||||_|||||:|\n______________GUUAG_____UUUGUU____UG_GU_GAG__UAAGG_GGUAAAG____U__AAAC_UUUGCGA\n3'-AUU...GCAAC_____AACCG______AGCA__G__A___AA_____________AAAC_UU____U_______AUUAA...CAA-5'\n_____________|_______________________________________________________________|\n_____________57______________________________________________________________38\n |
170 -- 176 |
15 -- 21 |
-29.8413 kcal/mol |
5.55001 kcal/mol |
15.9749 kcal/mol |
0 16@LB@ | |@LB@5'-GCC...AAAAA ACA A A A U UU U AUCAC...CAA-3'@LB@ CAAUC AAACAG AC CA CUC GUUCC CUAUUUC A UUUG AAACGUU@LB@ ||||| |||||: || || ||| :|||| |:||||| | |||| |||||:|@LB@ GUUAG UUUGUU UG GU GAG UAAGG GGUAAAG U AAAC UUUGCGA@LB@3'-AUU...GCAAC AACCG AGCA G A AA AAAC UU U AUUAA...CAA-5'@LB@ | |@LB@ 57 38@LB@@LB@@LB@Energy -9.91 kcal/mol@LB@Hybridization Energy -29.8413 kcal/mol@LB@Unfolding Energy - mRNA 5.55001 kcal/mol@LB@Unfolding Energy - sRNA 15.9749 kcal/mol@LB@@LB@Position - mRNA 1 -- 15@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 65 |
0.002089 |
0.123 |
bsu15330 |
sigG |
-7.32 |
0.735765 |
1 -- 8 |
133 -- 140 |
RNA polymerase sigma-G factor |
|
______________1_______9\n______________|_______|\n___________5'-________UUGAG...AUG-3'\n______________UUCGCGGC\n______________|:|||:||\n______________AGGCGUCG\n3'-AUU...GUAAC________UUCAA...CAA-5'\n_____________|________|\n_____________141______132\n |
2 -- 8 |
133 -- 139 |
-12.0754 kcal/mol |
1.63369 kcal/mol |
4.3229 kcal/mol |
1 9@LB@ | |@LB@ 5'- UUGAG...AUG-3'@LB@ UUCGCGGC@LB@ |:|||:||@LB@ AGGCGUCG@LB@3'-AUU...GUAAC UUCAA...CAA-5'@LB@ | |@LB@ 141 132@LB@@LB@@LB@Energy -7.32 kcal/mol@LB@Hybridization Energy -12.0754 kcal/mol@LB@Unfolding Energy - mRNA 1.63369 kcal/mol@LB@Unfolding Energy - sRNA 4.3229 kcal/mol@LB@@LB@Position - mRNA 1 -- 8@LB@Position - sRNA 133 -- 140@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 133 -- 139@LB@ |
| 77 |
0.002638 |
0.1315 |
bsu36790 |
ywmA |
-11.30 |
0.183092 |
1 -- 48 |
33 -- 81 |
Evidence 5: No homology to any previously reported sequences |
|
_____________0_______________49\n_____________|_______________|\n5'-CGA...GUUCA_______________AUAUA...GAC-3'\n______________UACCC_UUUCUUAUU\n______________|||||_|:|||||||\n______________AUGGG_AGAGAAUAA\n3'-AUU...UUAGC_____U_________GGGGU...CAA-5'\n_____________|_______________|\n_____________82______________32\n |
65 -- 71 |
43 -- 49 |
-14.8917 kcal/mol |
3.00255 kcal/mol |
4.80466 kcal/mol |
0 49@LB@ | |@LB@5'-CGA...GUUCA AUAUA...GAC-3'@LB@ UACCC UUUCUUAUU@LB@ ||||| |:|||||||@LB@ AUGGG AGAGAAUAA@LB@3'-AUU...UUAGC U GGGGU...CAA-5'@LB@ | |@LB@ 82 32@LB@@LB@@LB@Energy -11.30 kcal/mol@LB@Hybridization Energy -14.8917 kcal/mol@LB@Unfolding Energy - mRNA 3.00255 kcal/mol@LB@Unfolding Energy - sRNA 4.80466 kcal/mol@LB@@LB@Position - mRNA 1 -- 48@LB@Position - sRNA 33 -- 81@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 43 -- 49@LB@ |
| 95 |
0.003784 |
0.1541 |
bsu12910 |
proG |
-16.76 |
0.014152 |
1 -- 50 |
33 -- 83 |
pyrroline-5-carboxylate reductase 3 |
|
______________1______________________________________________________51\n______________|______________________________________________________|\n___________5'-________U_________UC_____A_U________A_______A_U________CUAUU...UUG-3'\n______________UUCGUU_C_GUUUU__CA__ACGAU_G_UAUCCAUC___UGUUC_C_CCGUUUCU\n______________||||||_|_:|:||__||__||:||_|_||:|||||___|:|||_|_||:|||||\n______________AAGCAA_G_UAGAA__GU__UGUUA_C_AUGGGUAG___AUAAG_G_GGUAAAGA\n3'-AUU...UACCG______C_U_____CC__U______G__________AGA________________AACUU...CAA-5'\n_____________|_______________________________________________________|\n_____________84______________________________________________________32\n |
44 -- 50 |
33 -- 39 |
-32.4348 kcal/mol |
4.82786 kcal/mol |
13.9589 kcal/mol |
1 51@LB@ | |@LB@ 5'- U UC A U A A U CUAUU...UUG-3'@LB@ UUCGUU C GUUUU CA ACGAU G UAUCCAUC UGUUC C CCGUUUCU@LB@ |||||| | :|:|| || ||:|| | ||:||||| |:||| | ||:|||||@LB@ AAGCAA G UAGAA GU UGUUA C AUGGGUAG AUAAG G GGUAAAGA@LB@3'-AUU...UACCG C U CC U G AGA AACUU...CAA-5'@LB@ | |@LB@ 84 32@LB@@LB@@LB@Energy -16.76 kcal/mol@LB@Hybridization Energy -32.4348 kcal/mol@LB@Unfolding Energy - mRNA 4.82786 kcal/mol@LB@Unfolding Energy - sRNA 13.9589 kcal/mol@LB@@LB@Position - mRNA 1 -- 50@LB@Position - sRNA 33 -- 83@LB@Position Seed - mRNA 44 -- 50@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 99 |
0.004027 |
0.1561 |
bsu13870 |
ykvZ |
-9.91 |
0.324242 |
1 -- 15 |
1 -- 17 |
LacI family transcriptional regulator |
|
______________1________________16\n______________|________________|\n___________5'-__________A______UUUGG...GAA-3'\n______________GUUUAAUUUC___UGUU\n______________|:||||||||___||||\n______________CGAAUUAAAG___ACAA\n3'-AUU...UUUUG__________AAA____-5'\n_____________|________________|\n_____________18_______________1\n |
4 -- 10 |
8 -- 14 |
-10.8546 kcal/mol |
0.387335 kcal/mol |
1.29438 kcal/mol |
1 16@LB@ | |@LB@ 5'- A UUUGG...GAA-3'@LB@ GUUUAAUUUC UGUU@LB@ |:|||||||| ||||@LB@ CGAAUUAAAG ACAA@LB@3'-AUU...UUUUG AAA -5'@LB@ | |@LB@ 18 1@LB@@LB@@LB@Energy -9.91 kcal/mol@LB@Hybridization Energy -10.8546 kcal/mol@LB@Unfolding Energy - mRNA 0.387335 kcal/mol@LB@Unfolding Energy - sRNA 1.29438 kcal/mol@LB@@LB@Position - mRNA 1 -- 15@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 4 -- 10@LB@Position Seed - sRNA 8 -- 14@LB@ |
| 132 |
0.006133 |
0.1741 |
bsu24800 |
yqgW |
-15.55 |
0.025469 |
1 -- 63 |
31 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
______________1_________________________________________________________________64\n______________|_________________________________________________________________|\n___________5'-__G____C_____________CCUACU____U____C________ACUA____CA___C_______UUUAU...CCG-3'\n______________GC_UUCG_UUG_AAUC__GGC______GGCA_UCGU_UCCAUUUC____UUAU__CCU_UUUCUUU\n______________||_||||_|||_||||__|||______::||_||||_:||||:||____||||__||:_|||||||\n______________CG_AAGC_AAC_UUAG__CCG______UUGU_AGCA_GGGUAGAG____AAUA__GGG_AAAGAAA\n3'-AUU...CUUAC___________G____AA___U_________U____U________________AG___U_______CUUUA...CAA-5'\n_____________|__________________________________________________________________|\n_____________86_________________________________________________________________30\n |
36 -- 42 |
48 -- 54 |
-38.6272 kcal/mol |
10.4973 kcal/mol |
14.833 kcal/mol |
1 64@LB@ | |@LB@ 5'- G C CCUACU U C ACUA CA C UUUAU...CCG-3'@LB@ GC UUCG UUG AAUC GGC GGCA UCGU UCCAUUUC UUAU CCU UUUCUUU@LB@ || |||| ||| |||| ||| ::|| |||| :||||:|| |||| ||: |||||||@LB@ CG AAGC AAC UUAG CCG UUGU AGCA GGGUAGAG AAUA GGG AAAGAAA@LB@3'-AUU...CUUAC G AA U U U AG U CUUUA...CAA-5'@LB@ | |@LB@ 86 30@LB@@LB@@LB@Energy -15.55 kcal/mol@LB@Hybridization Energy -38.6272 kcal/mol@LB@Unfolding Energy - mRNA 10.4973 kcal/mol@LB@Unfolding Energy - sRNA 14.833 kcal/mol@LB@@LB@Position - mRNA 1 -- 63@LB@Position - sRNA 31 -- 85@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 148 |
0.006782 |
0.1741 |
bsu14050 |
ykuE |
-11.57 |
0.163142 |
1 -- 16 |
37 -- 54 |
metallophosphoesterase |
|
______________1__________________________17\n______________|__________________________|\n___________5'-_____G____________GC_GC____CCCGC...AUC-3'\n______________CCAUC_CU__UUUCCCAU__C__UUGA\n______________|||||_||__||:|||||__|__||||\n______________GGUAG_GA__AAGGGGUA__G__AACU\n3'-AUU...GCAUG_____A__AU________AA_A_____UUAAA...CAA-5'\n_____________|___________________________|\n_____________55__________________________36\n |
10 -- 16 |
37 -- 43 |
-20.8225 kcal/mol |
1.63511 kcal/mol |
9.4766 kcal/mol |
1 17@LB@ | |@LB@ 5'- G GC GC CCCGC...AUC-3'@LB@ CCAUC CU UUUCCCAU C UUGA@LB@ ||||| || ||:||||| | ||||@LB@ GGUAG GA AAGGGGUA G AACU@LB@3'-AUU...GCAUG A AU AA A UUAAA...CAA-5'@LB@ | |@LB@ 55 36@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -20.8225 kcal/mol@LB@Unfolding Energy - mRNA 1.63511 kcal/mol@LB@Unfolding Energy - sRNA 9.4766 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 178 |
0.008149 |
0.1751 |
bsu39610 |
yxeB |
-9.68 |
0.354141 |
1 -- 23 |
37 -- 58 |
iron(3+)-hydroxamate-binding protein YxeB |
bsu33320 |
_____________0_______24\n_____________|_______|\n5'-GCA...UGUAA_______AUUUU...CUG-3'\n______________UUCCCAU\n______________|:|||||\n______________AGGGGUA\n3'-AUU...GAAUA_______AAGAA...CAA-5'\n_____________|_______|\n_____________59______36\n |
17 -- 23 |
37 -- 43 |
-9.25042 kcal/mol |
0.0358808 kcal/mol |
2.53706 kcal/mol |
0 24@LB@ | |@LB@5'-GCA...UGUAA AUUUU...CUG-3'@LB@ UUCCCAU@LB@ |:|||||@LB@ AGGGGUA@LB@3'-AUU...GAAUA AAGAA...CAA-5'@LB@ | |@LB@ 59 36@LB@@LB@@LB@Energy -9.68 kcal/mol@LB@Hybridization Energy -9.25042 kcal/mol@LB@Unfolding Energy - mRNA 0.0358808 kcal/mol@LB@Unfolding Energy - sRNA 2.53706 kcal/mol@LB@@LB@Position - mRNA 1 -- 23@LB@Position - sRNA 37 -- 58@LB@Position Seed - mRNA 17 -- 23@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 52 |
0.001732 |
0.1212 |
bsu29530 |
sppA |
-10.15 |
0.295539 |
2 -- 29 |
39 -- 81 |
signal peptide peptidase SppA |
|
_____________1________________________________________________________30\n_____________|________________________________________________________|\n5'-GCG...CUCUG_G______C____UGUCUAUU_____CAG__________GA_______________GCGUC...AAG-3'\n______________G_GAUUUU_GGCG________AUCGU___CAUCUC_UAU_____GUUUCUUUGAAA\n______________|_:||:||_|||:________|||||___||||||_|||_____:|||||||||||\n______________C_UUAGAA_CCGU________UAGCA___GUAGAG_AUA_____UAAAGAAACUUU\n3'-AUU...AGCAA_G___________UUGU_________UGG______A___AGGGG____________AAACU...CAA-5'\n_____________|________________________________________________________|\n_____________82_______________________________________________________38\n |
284 -- 290 |
28 -- 34 |
-34.8894 kcal/mol |
12.7856 kcal/mol |
14.4529 kcal/mol |
1 30@LB@ | |@LB@5'-GCG...CUCUG G C UGUCUAUU CAG GA GCGUC...AAG-3'@LB@ G GAUUUU GGCG AUCGU CAUCUC UAU GUUUCUUUGAAA@LB@ | :||:|| |||: ||||| |||||| ||| :|||||||||||@LB@ C UUAGAA CCGU UAGCA GUAGAG AUA UAAAGAAACUUU@LB@3'-AUU...AGCAA G UUGU UGG A AGGGG AAACU...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -10.15 kcal/mol@LB@Hybridization Energy -34.8894 kcal/mol@LB@Unfolding Energy - mRNA 12.7856 kcal/mol@LB@Unfolding Energy - sRNA 14.4529 kcal/mol@LB@@LB@Position - mRNA 2 -- 29@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 28 -- 34@LB@ |
