| 51 |
0.001644 |
0.1212 |
bsu29870 |
ytzB |
-11.72 |
0.152375 |
23 -- 59 |
33 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________22__________________________________________60\n_____________|___________________________________________|\n5'-CUG...AUUGC___________________________________________AAAAG...AAG-3'\n______________UUAACAGGAU_UUGGC__UGUU__________UCUGUAUAAUG\n______________|:|||||||:_|||:|__|||:__________||::|||||||\n______________AGUUGUCCUG_AACUG__ACAG__________AGGUAUAUUAC\n3'-AUU...GAAGU__________U_____UA____GCGUCGUUCA___________AACAG...CAA-5'\n_____________|___________________________________________|\n_____________86__________________________________________32\n |
163 -- 169 |
118 -- 124 |
-27.3559 kcal/mol |
7.06665 kcal/mol |
10.6143 kcal/mol |
22 60@LB@ | |@LB@5'-CUG...AUUGC AAAAG...AAG-3'@LB@ UUAACAGGAU UUGGC UGUU UCUGUAUAAUG@LB@ |:|||||||: |||:| |||: ||::|||||||@LB@ AGUUGUCCUG AACUG ACAG AGGUAUAUUAC@LB@3'-AUU...GAAGU U UA GCGUCGUUCA AACAG...CAA-5'@LB@ | |@LB@ 86 32@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -27.3559 kcal/mol@LB@Unfolding Energy - mRNA 7.06665 kcal/mol@LB@Unfolding Energy - sRNA 10.6143 kcal/mol@LB@@LB@Position - mRNA 23 -- 59@LB@Position - sRNA 33 -- 85@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 183 |
0.008402 |
0.1751 |
bsu03010 |
amhX |
-13.44 |
0.069669 |
23 -- 92 |
7 -- 86 |
amidohydrolase AmhX |
|
_____________22_______________________________________________________________________________________93\n_____________|________________________________________________________________________________________|\n5'-AAA...UCUUA____G____U______GC____________________AU___GAUCCA__________________________A______UA____GAAUA...CUU-3'\n______________GGCU_UGUU_CGG_CU__UGGCA________UACUCAU__UUC______UCCCC___UCU_____GUUUGAAAAU__UUAAU__UUCU\n______________||||_:|||_|::_||__|||||________|||:|||__:||______|||||___|||_____:||||||||:__|||||__||||\n______________CCGA_GCAA_GUU_GA__ACCGU________AUGGGUA__GAG______AGGGG___AGA_____UAAACUUUUG__AAUUA__AAGA\n3'-AUU...ACUUA____A____C___A_________UUGUUAGC____________AAUA_______UAA___AACUU__________CG___________AAACAA-5'\n_____________|________________________________________________________________________________________|\n_____________87_______________________________________________________________________________________6\n |
45 -- 51 |
51 -- 57 |
-41.3184 kcal/mol |
11.5757 kcal/mol |
17.8015 kcal/mol |
22 93@LB@ | |@LB@5'-AAA...UCUUA G U GC AU GAUCCA A UA GAAUA...CUU-3'@LB@ GGCU UGUU CGG CU UGGCA UACUCAU UUC UCCCC UCU GUUUGAAAAU UUAAU UUCU@LB@ |||| :||| |:: || ||||| |||:||| :|| ||||| ||| :||||||||: ||||| ||||@LB@ CCGA GCAA GUU GA ACCGU AUGGGUA GAG AGGGG AGA UAAACUUUUG AAUUA AAGA@LB@3'-AUU...ACUUA A C A UUGUUAGC AAUA UAA AACUU CG AAACAA-5'@LB@ | |@LB@ 87 6@LB@@LB@@LB@Energy -13.44 kcal/mol@LB@Hybridization Energy -41.3184 kcal/mol@LB@Unfolding Energy - mRNA 11.5757 kcal/mol@LB@Unfolding Energy - sRNA 17.8015 kcal/mol@LB@@LB@Position - mRNA 23 -- 92@LB@Position - sRNA 7 -- 86@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 51 -- 57@LB@ |
| 186 |
0.00854 |
0.1751 |
bsu03320 |
nasB |
-11.11 |
0.199178 |
25 -- 36 |
33 -- 45 |
assimilatory nitrate reductase electron transfer subunit |
bsu03300 |
_____________24____________37\n_____________|_____________|\n5'-UUU...AUAAG_____________AGACC...UCA-3'\n______________AUUCCCC_UUUCU\n______________|||||||_|||||\n______________UAAGGGG_AAAGA\n3'-AUU...GAGAA_______U_____AACUU...CAA-5'\n_____________|_____________|\n_____________46____________32\n |
25 -- 31 |
39 -- 45 |
-15.5675 kcal/mol |
1.76448 kcal/mol |
4.94568 kcal/mol |
24 37@LB@ | |@LB@5'-UUU...AUAAG AGACC...UCA-3'@LB@ AUUCCCC UUUCU@LB@ ||||||| |||||@LB@ UAAGGGG AAAGA@LB@3'-AUU...GAGAA U AACUU...CAA-5'@LB@ | |@LB@ 46 32@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -15.5675 kcal/mol@LB@Unfolding Energy - mRNA 1.76448 kcal/mol@LB@Unfolding Energy - sRNA 4.94568 kcal/mol@LB@@LB@Position - mRNA 25 -- 36@LB@Position - sRNA 33 -- 45@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 5 |
0.0001198 |
0.08823 |
bsu33680 |
yvaP |
-23.88 |
0.000393 |
26 -- 134 |
34 -- 167 |
HTH-type transcriptional regulator YvaP |
|
_____________25___________________________________________________________________________________________________________________________________________________________135\n_____________|____________________________________________________________________________________________________________________________________________________________|\n5'-UUU...AACAU_______A______________AAA____________AUA________________A_______________AGCAAAAAAAC__________C_____C__UC______C___________CU____A______AUCAAUC______________CAUGC...CGC-3'\n______________CUC_UCA__AGCAGGAUAUUGA_____UCU_CAGCAA___CU___________UCG_UUUUUUGUCUUCAGA___________GC______GC__UCUU__C__AUAAUC___CCUGUUUCU__UUCU_UAUUUU______________ACGCUUG\n______________|||_|||__|:|||||:|||||_____||:_||||||___|:___________||:_|||:|::||||||:|___________||______||__||||__|__|:||||___||::|:|||__|||:_:||||:______________||||||:\n______________GAG_AGU__UUGUCCUGUAACU_____AGG_GUCGUU___GG___________AGU_AAAGAGUAGAAGUUU___________CG______CG__AGAA__G__UGUUAG___GGGUAGAGA__AAGG_GUAAAG______________UGCGAAU\n3'-AUU...AAAAU___A___AG_____________GUAAC___C______CAA__UAUAUUACAAC___G_______________CAUUACUUAC___AAGCAA__UU____CC_UU______CAU_________AU____G______AAACUUUAAACUUU_______UAAAG...CAA-5'\n_____________|____________________________________________________________________________________________________________________________________________________________|\n_____________168__________________________________________________________________________________________________________________________________________________________33\n |
142 -- 148 |
13 -- 19 |
-70.4657 kcal/mol |
17.0109 kcal/mol |
33.4152 kcal/mol |
25 135@LB@ | |@LB@5'-UUU...AACAU A AAA AUA A AGCAAAAAAAC C C UC C CU A AUCAAUC CAUGC...CGC-3'@LB@ CUC UCA AGCAGGAUAUUGA UCU CAGCAA CU UCG UUUUUUGUCUUCAGA GC GC UCUU C AUAAUC CCUGUUUCU UUCU UAUUUU ACGCUUG@LB@ ||| ||| |:|||||:||||| ||: |||||| |: ||: |||:|::||||||:| || || |||| | |:|||| ||::|:||| |||: :||||: ||||||:@LB@ GAG AGU UUGUCCUGUAACU AGG GUCGUU GG AGU AAAGAGUAGAAGUUU CG CG AGAA G UGUUAG GGGUAGAGA AAGG GUAAAG UGCGAAU@LB@3'-AUU...AAAAU A AG GUAAC C CAA UAUAUUACAAC G CAUUACUUAC AAGCAA UU CC UU CAU AU G AAACUUUAAACUUU UAAAG...CAA-5'@LB@ | |@LB@ 168 33@LB@@LB@@LB@Energy -23.88 kcal/mol@LB@Hybridization Energy -70.4657 kcal/mol@LB@Unfolding Energy - mRNA 17.0109 kcal/mol@LB@Unfolding Energy - sRNA 33.4152 kcal/mol@LB@@LB@Position - mRNA 26 -- 134@LB@Position - sRNA 34 -- 167@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 20 |
0.0005654 |
0.1011 |
bsu03960 |
ycnK |
-22.14 |
0.000955 |
26 -- 173 |
12 -- 134 |
DeoR family transcriptional regulator |
|
_____________25________________________________________________________________________________________________________________________________________________________174\n_____________|_________________________________________________________________________________________________________________________________________________________|\n5'-AGG...GUUUC_______GGGUAUUCC_________AUUC_____GUCUAUUAUAC______CCCUUC__UU_C__________U__________C___UUUUCACAUUUUUU__________C__A____________C___A______CCUCU_________AUAGA...AAA-3'\n______________UUUCAUA_________UCACUUUCU____CAUCU___________CAGAGU______UG__U_GUUUUGUUGC_GUU____CAA_ACA______________AUC__AUCCA_UU_CUUG_UCUCCGU_UCU___AAAU______ACGCUUAA\n______________||:||||_________|||||||||____|||||___________||:|||______||__|_|:||:|||||_:|:____|||_|||______________|||__|:|||_:|_|||:_||:||:|_|||___||||______||||||||\n______________AAGGUAU_________AGUGAAAGA____GUAGA___________GUUUCA______AC__A_CGAAGCAACG_UAG____GUU_UGU______________UAG__UGGGU_GA_GAAU_AGGGGUA_AGA___UUUA______UGCGAAUU\n3'-AUU...CGUUC_______AUUACAAC___________________A________________UU______UU_C__________U___AACC________________________CA_____A_______A_______A___AAC____AACUUU________AAAGA...CAA-5'\n_____________|_________________________________________________________________________________________________________________________________________________________|\n_____________135_______________________________________________________________________________________________________________________________________________________11\n |
167 -- 173 |
12 -- 18 |
-57.1101 kcal/mol |
10.5528 kcal/mol |
25.6552 kcal/mol |
25 174@LB@ | |@LB@5'-AGG...GUUUC GGGUAUUCC AUUC GUCUAUUAUAC CCCUUC UU C U C UUUUCACAUUUUUU C A C A CCUCU AUAGA...AAA-3'@LB@ UUUCAUA UCACUUUCU CAUCU CAGAGU UG U GUUUUGUUGC GUU CAA ACA AUC AUCCA UU CUUG UCUCCGU UCU AAAU ACGCUUAA@LB@ ||:|||| ||||||||| ||||| ||:||| || | |:||:||||| :|: ||| ||| ||| |:||| :| |||: ||:||:| ||| |||| ||||||||@LB@ AAGGUAU AGUGAAAGA GUAGA GUUUCA AC A CGAAGCAACG UAG GUU UGU UAG UGGGU GA GAAU AGGGGUA AGA UUUA UGCGAAUU@LB@3'-AUU...CGUUC AUUACAAC A UU UU C U AACC CA A A A AAC AACUUU AAAGA...CAA-5'@LB@ | |@LB@ 135 11@LB@@LB@@LB@Energy -22.14 kcal/mol@LB@Hybridization Energy -57.1101 kcal/mol@LB@Unfolding Energy - mRNA 10.5528 kcal/mol@LB@Unfolding Energy - sRNA 25.6552 kcal/mol@LB@@LB@Position - mRNA 26 -- 173@LB@Position - sRNA 12 -- 134@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 12 -- 18@LB@ |
| 156 |
0.007194 |
0.1751 |
bsu12900 |
htrA |
-15.16 |
0.030869 |
26 -- 118 |
38 -- 130 |
serine protease Do-like HtrA |
|
_____________25_______________________________________________________________________________________________________119\n_____________|________________________________________________________________________________________________________|\n5'-CUG...AAUCU_______CGACUCA__________A___A_____U________GU__AC____UUCC___C____UC_________U_______AA____UUCAUUUUUA____ACAGU...AUG-3'\n______________GUUCCAU________GUC_CUUUC_UAU___CAA____AUGAA__GU__CGUU____GCA_UUUU_____ACAAUU___CCCAU__UCUU__________UCCC\n______________|||||||________|||_|||||_:||___|||____|||||__|:__||||____|||_|:||_____|||||:___|||||__||||__________||||\n______________CAAGGUA________CAG_GAAAG_GUA___GUU____UACUU__CG__GCAA____CGU_AGAA_____UGUUAG___GGGUA__AGAA__________AGGG\n3'-AUU...UCGUU_______UAUUACAA___U_____A___GAA___UCAU_____AC__AA___________U____CCGUU______CAU_____G_____UA____________GUAAA...CAA-5'\n_____________|________________________________________________________________________________________________________|\n_____________131______________________________________________________________________________________________________37\n |
26 -- 32 |
124 -- 130 |
-40.6941 kcal/mol |
8.96571 kcal/mol |
22.8172 kcal/mol |
25 119@LB@ | |@LB@5'-CUG...AAUCU CGACUCA A A U GU AC UUCC C UC U AA UUCAUUUUUA ACAGU...AUG-3'@LB@ GUUCCAU GUC CUUUC UAU CAA AUGAA GU CGUU GCA UUUU ACAAUU CCCAU UCUU UCCC@LB@ ||||||| ||| ||||| :|| ||| ||||| |: |||| ||| |:|| |||||: ||||| |||| ||||@LB@ CAAGGUA CAG GAAAG GUA GUU UACUU CG GCAA CGU AGAA UGUUAG GGGUA AGAA AGGG@LB@3'-AUU...UCGUU UAUUACAA U A GAA UCAU AC AA U CCGUU CAU G UA GUAAA...CAA-5'@LB@ | |@LB@ 131 37@LB@@LB@@LB@Energy -15.16 kcal/mol@LB@Hybridization Energy -40.6941 kcal/mol@LB@Unfolding Energy - mRNA 8.96571 kcal/mol@LB@Unfolding Energy - sRNA 22.8172 kcal/mol@LB@@LB@Position - mRNA 26 -- 118@LB@Position - sRNA 38 -- 130@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 124 -- 130@LB@ |
| 48 |
0.001494 |
0.1206 |
bsu33660 |
rghRA |
-13.24 |
0.076349 |
28 -- 146 |
39 -- 166 |
HTH-type transcriptional repressor RghR |
bsu33670 |
_____________27_________________________________________________147\n_____________|__________________________________________________|\n5'-UAU...AUUAC_____A____AAC______U___U___U_______A____A_C_______UUUAG...UCU-3'\n______________GGCUU_UGUU____UUUUG_CAA_ACA_UU___CC_GUCU_C_UAUUCCC\n______________|||||_:|||____|:|||_|||_|||_|:___||_:|||_|_|||||||\n______________CCGAA_GCAA____AGAAC_GUU_UGU_AG___GG_UAGA_G_AUAAGGG\n3'-AUU...ACUUA__________CGUU_____C_______U__CAU__G______A_______GUAAA...CAA-5'\n_____________|__________________________________________________|\n_____________167________________________________________________38\n |
139 -- 145 |
40 -- 46 |
-27.8785 kcal/mol |
6.21563 kcal/mol |
10.9223 kcal/mol |
27 147@LB@ | |@LB@5'-UAU...AUUAC A AAC U U U A A C UUUAG...UCU-3'@LB@ GGCUU UGUU UUUUG CAA ACA UU CC GUCU C UAUUCCC@LB@ ||||| :||| |:||| ||| ||| |: || :||| | |||||||@LB@ CCGAA GCAA AGAAC GUU UGU AG GG UAGA G AUAAGGG@LB@3'-AUU...ACUUA CGUU C U CAU G A GUAAA...CAA-5'@LB@ | |@LB@ 167 38@LB@@LB@@LB@Energy -13.24 kcal/mol@LB@Hybridization Energy -27.8785 kcal/mol@LB@Unfolding Energy - mRNA 6.21563 kcal/mol@LB@Unfolding Energy - sRNA 10.9223 kcal/mol@LB@@LB@Position - mRNA 28 -- 146@LB@Position - sRNA 39 -- 166@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 171 |
0.007883 |
0.1751 |
bsu38330 |
lrgB |
-10.47 |
0.260275 |
28 -- 48 |
159 -- 187 |
'Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 15659658 15849754 16850406; Product type r : regulator' |
|
_____________27____________49\n_____________|_____________|\n5'-GAG...CUGCU_____________CCGUC...UAU-3'\n______________UUUAUUUUUUAUC\n______________||||:|:||:|||\n______________AAAUGAGAAGUAG\n3'-AUU...AAAAA_____________UUGUC...CAA-5'\n_____________|_____________|\n_____________188___________158\n |
42 -- 48 |
159 -- 165 |
-9.66314 kcal/mol |
1.00478 kcal/mol |
0.734181 kcal/mol |
27 49@LB@ | |@LB@5'-GAG...CUGCU CCGUC...UAU-3'@LB@ UUUAUUUUUUAUC@LB@ ||||:|:||:|||@LB@ AAAUGAGAAGUAG@LB@3'-AUU...AAAAA UUGUC...CAA-5'@LB@ | |@LB@ 188 158@LB@@LB@@LB@Energy -10.47 kcal/mol@LB@Hybridization Energy -9.66314 kcal/mol@LB@Unfolding Energy - mRNA 1.00478 kcal/mol@LB@Unfolding Energy - sRNA 0.734181 kcal/mol@LB@@LB@Position - mRNA 28 -- 48@LB@Position - sRNA 159 -- 187@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 159 -- 165@LB@ |
| 3 |
8.73e-05 |
0.08823 |
bsu25090 |
yqfW |
-16.63 |
0.015052 |
29 -- 79 |
33 -- 81 |
nucleotidase YqfW |
|
_____________28____________________________________________________80\n_____________|_____________________________________________________|\n5'-CCU...GUAAC______U___AC___UUUUCG_______UUG___C___CA_______C_____GUUUU...ACG-3'\n______________UGUUGU_AUC___GC______UAAUCGU___CCA_CUU__AUUCCCC__UUCU\n______________:||||:_|||___||______:||||||___|||_||:__|||||||__||||\n______________GCAACG_UAG___CG______GUUAGCA___GGU_GAG__UAAGGGG__AAGA\n3'-AUU...CCGAA______U___AAC__UUU__________UG____A___AA_______UA____AACUU...CAA-5'\n_____________|_____________________________________________________|\n_____________82____________________________________________________32\n |
68 -- 74 |
39 -- 45 |
-32.9438 kcal/mol |
5.67451 kcal/mol |
13.9362 kcal/mol |
28 80@LB@ | |@LB@5'-CCU...GUAAC U AC UUUUCG UUG C CA C GUUUU...ACG-3'@LB@ UGUUGU AUC GC UAAUCGU CCA CUU AUUCCCC UUCU@LB@ :||||: ||| || :|||||| ||| ||: ||||||| ||||@LB@ GCAACG UAG CG GUUAGCA GGU GAG UAAGGGG AAGA@LB@3'-AUU...CCGAA U AAC UUU UG A AA UA AACUU...CAA-5'@LB@ | |@LB@ 82 32@LB@@LB@@LB@Energy -16.63 kcal/mol@LB@Hybridization Energy -32.9438 kcal/mol@LB@Unfolding Energy - mRNA 5.67451 kcal/mol@LB@Unfolding Energy - sRNA 13.9362 kcal/mol@LB@@LB@Position - mRNA 29 -- 79@LB@Position - sRNA 33 -- 81@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 115 |
0.005245 |
0.1741 |
bsu07690 |
yflG |
-14.30 |
0.046489 |
29 -- 56 |
32 -- 57 |
methionine aminopeptidase 2 |
|
_____________28___________________________57\n_____________|____________________________|\n5'-UUC...AAGCA________CCC__C__A____U______CAAAU...GCA-3'\n______________UACCCAUC___CU_AU_CCCU_UUUUUU\n______________||||||||___||_||_|||:_|||:||\n______________AUGGGUAG___GA_UA_GGGG_AAAGAA\n3'-AUU...UUAGC________A____A__A____U______ACUUU...CAA-5'\n_____________|____________________________|\n_____________58___________________________31\n |
30 -- 36 |
50 -- 56 |
-21.2542 kcal/mol |
0.575621 kcal/mol |
9.1585 kcal/mol |
28 57@LB@ | |@LB@5'-UUC...AAGCA CCC C A U CAAAU...GCA-3'@LB@ UACCCAUC CU AU CCCU UUUUUU@LB@ |||||||| || || |||: |||:||@LB@ AUGGGUAG GA UA GGGG AAAGAA@LB@3'-AUU...UUAGC A A A U ACUUU...CAA-5'@LB@ | |@LB@ 58 31@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -21.2542 kcal/mol@LB@Unfolding Energy - mRNA 0.575621 kcal/mol@LB@Unfolding Energy - sRNA 9.1585 kcal/mol@LB@@LB@Position - mRNA 29 -- 56@LB@Position - sRNA 32 -- 57@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 50 -- 56@LB@ |
