| 19 |
0.0005622 |
0.1011 |
bsu33310 |
fhuB |
-20.25 |
0.002499 |
30 -- 116 |
99 -- 184 |
iron(3+)-hydroxamate import system permease protein FhuB |
bsu01620 |
_____________29__________________________________________________________________117\n_____________|___________________________________________________________________|\n5'-AGG...UGCAA___A__________AAAAA____________UCC_______UU_____U___G______A_______ACCUU...AAG-3'\n______________UCA_UAACAGGGCA___________CGCGGC___GAGUUUC__GUAUA_AUG__GUCAU__UCUCAU\n______________|||_:||||||:||___________|||:||___:||||:|__:||||_|||__||||:__||||||\n______________AGU_GUUGUCCUGU___________GCGUCG___UUCAAGG__UAUAU_UAC__CAGUG__AGAGUA\n3'-AUU...UGAGA___A__________AACUGUAACAG___________________________AA_____AA______GAAGU...CAA-5'\n_____________|___________________________________________________________________|\n_____________185_________________________________________________________________98\n |
80 -- 86 |
126 -- 132 |
-44.6592 kcal/mol |
13.3222 kcal/mol |
14.475 kcal/mol |
29 117@LB@ | |@LB@5'-AGG...UGCAA A AAAAA UCC UU U G A ACCUU...AAG-3'@LB@ UCA UAACAGGGCA CGCGGC GAGUUUC GUAUA AUG GUCAU UCUCAU@LB@ ||| :||||||:|| |||:|| :||||:| :|||| ||| ||||: ||||||@LB@ AGU GUUGUCCUGU GCGUCG UUCAAGG UAUAU UAC CAGUG AGAGUA@LB@3'-AUU...UGAGA A AACUGUAACAG AA AA GAAGU...CAA-5'@LB@ | |@LB@ 185 98@LB@@LB@@LB@Energy -20.25 kcal/mol@LB@Hybridization Energy -44.6592 kcal/mol@LB@Unfolding Energy - mRNA 13.3222 kcal/mol@LB@Unfolding Energy - sRNA 14.475 kcal/mol@LB@@LB@Position - mRNA 30 -- 116@LB@Position - sRNA 99 -- 184@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 126 -- 132@LB@ |
| 14 |
0.0004671 |
0.1011 |
bsu04470 |
dctP |
-11.58 |
0.162472 |
124 -- 147 |
15 -- 44 |
C4-dicarboxylate transport protein |
bsu02340 bsu10220 |
_____________123____________________________148\n_____________|______________________________|\n5'-AAA...UAAAC_______GA__________A__________AUCAU...AAC-3'\n______________UUCCCCA___CU_____GU_UGAAAACGCU\n______________|||||||___||_____:|_||||||||||\n______________AAGGGGU___GA_____UA_ACUUUUGCGA\n3'-AUU...AGAAU_______AAA__AACUU__A__________AUUAA...CAA-5'\n_____________|______________________________|\n_____________45_____________________________14\n |
141 -- 147 |
15 -- 21 |
-23.6241 kcal/mol |
3.82582 kcal/mol |
8.2119 kcal/mol |
123 148@LB@ | |@LB@5'-AAA...UAAAC GA A AUCAU...AAC-3'@LB@ UUCCCCA CU GU UGAAAACGCU@LB@ ||||||| || :| ||||||||||@LB@ AAGGGGU GA UA ACUUUUGCGA@LB@3'-AUU...AGAAU AAA AACUU A AUUAA...CAA-5'@LB@ | |@LB@ 45 14@LB@@LB@@LB@Energy -11.58 kcal/mol@LB@Hybridization Energy -23.6241 kcal/mol@LB@Unfolding Energy - mRNA 3.82582 kcal/mol@LB@Unfolding Energy - sRNA 8.2119 kcal/mol@LB@@LB@Position - mRNA 124 -- 147@LB@Position - sRNA 15 -- 44@LB@Position Seed - mRNA 141 -- 147@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 186 |
0.00854 |
0.1751 |
bsu03320 |
nasB |
-11.11 |
0.199178 |
25 -- 36 |
33 -- 45 |
assimilatory nitrate reductase electron transfer subunit |
bsu03300 |
_____________24____________37\n_____________|_____________|\n5'-UUU...AUAAG_____________AGACC...UCA-3'\n______________AUUCCCC_UUUCU\n______________|||||||_|||||\n______________UAAGGGG_AAAGA\n3'-AUU...GAGAA_______U_____AACUU...CAA-5'\n_____________|_____________|\n_____________46____________32\n |
25 -- 31 |
39 -- 45 |
-15.5675 kcal/mol |
1.76448 kcal/mol |
4.94568 kcal/mol |
24 37@LB@ | |@LB@5'-UUU...AUAAG AGACC...UCA-3'@LB@ AUUCCCC UUUCU@LB@ ||||||| |||||@LB@ UAAGGGG AAAGA@LB@3'-AUU...GAGAA U AACUU...CAA-5'@LB@ | |@LB@ 46 32@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -15.5675 kcal/mol@LB@Unfolding Energy - mRNA 1.76448 kcal/mol@LB@Unfolding Energy - sRNA 4.94568 kcal/mol@LB@@LB@Position - mRNA 25 -- 36@LB@Position - sRNA 33 -- 45@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 152 |
0.006831 |
0.1741 |
bsu09480 |
yhdI |
-9.89 |
0.327778 |
76 -- 101 |
102 -- 130 |
GntR family transcriptional regulator |
bsu05240 |
_____________75__________________________102\n_____________|___________________________|\n5'-UCU...UAAUU___________A________C______AUCCC...CAA-3'\n______________GUUUCAUAUGG_____UCAU_UUCUCA\n______________|||:|||||::_____|||:_||||||\n______________CAAGGUAUAUU_____AGUG_AAGAGU\n3'-AUU...UCGUU___________ACAAC____A______AGAAG...CAA-5'\n_____________|___________________________|\n_____________131_________________________101\n |
79 -- 85 |
121 -- 127 |
-18.1477 kcal/mol |
2.87073 kcal/mol |
6.25825 kcal/mol |
75 102@LB@ | |@LB@5'-UCU...UAAUU A C AUCCC...CAA-3'@LB@ GUUUCAUAUGG UCAU UUCUCA@LB@ |||:|||||:: |||: ||||||@LB@ CAAGGUAUAUU AGUG AAGAGU@LB@3'-AUU...UCGUU ACAAC A AGAAG...CAA-5'@LB@ | |@LB@ 131 101@LB@@LB@@LB@Energy -9.89 kcal/mol@LB@Hybridization Energy -18.1477 kcal/mol@LB@Unfolding Energy - mRNA 2.87073 kcal/mol@LB@Unfolding Energy - sRNA 6.25825 kcal/mol@LB@@LB@Position - mRNA 76 -- 101@LB@Position - sRNA 102 -- 130@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 109 |
0.004595 |
0.1633 |
bsu02700 |
estA |
-7.85 |
0.646397 |
289 -- 295 |
181 -- 187 |
lipase EstA |
bsu08350 |
_____________288_______296\n_____________|_________|\n5'-AUC...CUUAA_________AAAAA...AAC-3'\n______________AAUUUCUUU\n______________|||||||||\n______________UUAAAGAAA\n3'-AUU...GCGAA_________ACAA-5'\n_____________|_________|\n_____________188_______180\n |
109 -- 115 |
5 -- 11 |
-6.84044 kcal/mol |
1.42932 kcal/mol |
0.176622 kcal/mol |
288 296@LB@ | |@LB@5'-AUC...CUUAA AAAAA...AAC-3'@LB@ AAUUUCUUU@LB@ |||||||||@LB@ UUAAAGAAA@LB@3'-AUU...GCGAA ACAA-5'@LB@ | |@LB@ 188 180@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -6.84044 kcal/mol@LB@Unfolding Energy - mRNA 1.42932 kcal/mol@LB@Unfolding Energy - sRNA 0.176622 kcal/mol@LB@@LB@Position - mRNA 289 -- 295@LB@Position - sRNA 181 -- 187@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 5 -- 11@LB@ |
| 10 |
0.0002277 |
0.08823 |
bsu05690 |
ydhB |
-21.23 |
0.001523 |
113 -- 150 |
136 -- 182 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
bsu12400 |
_____________112_______________________________151\n_____________|_________________________________|\n5'-CAU...UGUUU__________________C______ACC_____CAGAA...UCC-3'\n______________UUCUUCGUCAAUAGGACA____CAU___UCCGC\n______________:|||||:||||:||||||____|||___|||||\n______________GAGAAGUAGUUGUCCUGU____GUA___AGGCG\n3'-AUU...AAAAU__________________AACU___AC______UCGUU...CAA-5'\n_____________|_________________________________|\n_____________183_______________________________135\n |
132 -- 138 |
150 -- 156 |
-33.3473 kcal/mol |
6.29637 kcal/mol |
7.50175 kcal/mol |
112 151@LB@ | |@LB@5'-CAU...UGUUU C ACC CAGAA...UCC-3'@LB@ UUCUUCGUCAAUAGGACA CAU UCCGC@LB@ :|||||:||||:|||||| ||| |||||@LB@ GAGAAGUAGUUGUCCUGU GUA AGGCG@LB@3'-AUU...AAAAU AACU AC UCGUU...CAA-5'@LB@ | |@LB@ 183 135@LB@@LB@@LB@Energy -21.23 kcal/mol@LB@Hybridization Energy -33.3473 kcal/mol@LB@Unfolding Energy - mRNA 6.29637 kcal/mol@LB@Unfolding Energy - sRNA 7.50175 kcal/mol@LB@@LB@Position - mRNA 113 -- 150@LB@Position - sRNA 136 -- 182@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 150 -- 156@LB@ |
| 112 |
0.00483 |
0.1671 |
bsu26010 |
yqbQ |
-18.75 |
0.005285 |
180 -- 239 |
82 -- 139 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu12700 |
_____________179______________________________________________________________240\n_____________|________________________________________________________________|\n5'-CUA...AAAUA_______________G______ACA__AUAGA___________A______AAACG_________GAGCU...UUA-3'\n______________CCGCAGUAAG___CA_GUGAUG___UG_____AC_UUUUCGUC_UUAAAG_____GAAUGGCUU\n______________||||||:|||___||_:|:|||___||_____||_||:||:||_|:||||_____|||||||||\n______________GGCGUCGUUC___GU_UAUUAC___AC_____UG_AAGAGUAG_AGUUUC_____CUUACCGAA\n3'-AUU...UAACA__________AAG__A______A____AG_____A________A______AUUA__________GCAAC...CAA-5'\n_____________|________________________________________________________________|\n_____________140______________________________________________________________81\n |
233 -- 239 |
82 -- 88 |
-46.2512 kcal/mol |
11.9879 kcal/mol |
18.3017 kcal/mol |
179 240@LB@ | |@LB@5'-CUA...AAAUA G ACA AUAGA A AAACG GAGCU...UUA-3'@LB@ CCGCAGUAAG CA GUGAUG UG AC UUUUCGUC UUAAAG GAAUGGCUU@LB@ ||||||:||| || :|:||| || || ||:||:|| |:|||| |||||||||@LB@ GGCGUCGUUC GU UAUUAC AC UG AAGAGUAG AGUUUC CUUACCGAA@LB@3'-AUU...UAACA AAG A A AG A A AUUA GCAAC...CAA-5'@LB@ | |@LB@ 140 81@LB@@LB@@LB@Energy -18.75 kcal/mol@LB@Hybridization Energy -46.2512 kcal/mol@LB@Unfolding Energy - mRNA 11.9879 kcal/mol@LB@Unfolding Energy - sRNA 18.3017 kcal/mol@LB@@LB@Position - mRNA 180 -- 239@LB@Position - sRNA 82 -- 139@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 82 -- 88@LB@ |
| 145 |
0.006739 |
0.1741 |
bsu12460 |
xlyB |
-11.80 |
0.146979 |
21 -- 64 |
155 -- 186 |
N-acetylmuramoyl-L-alanine amidase XlyB |
bsu12810 bsu25710 bsu25900 bsu21410 |
_____________20____________________________________65\n_____________|_____________________________________|\n5'-UCC...CCACA_____C_G_____AUUUCUC____CU_______A___UUAAA...CGG-3'\n______________UGCUC_U_AUUAA_______CGUU__CAUUGUC_GCA\n______________|:|||_|_||:||_______|:||__|||||||_|||\n______________AUGAG_A_UAGUU_______GUAA__GUAACAG_CGU\n3'-AUU...AAAAA_____A_G_____GUCCU______CU_______G___CGUUC...CAA-5'\n_____________|_____________________________________|\n_____________187___________________________________154\n |
54 -- 60 |
139 -- 145 |
-22.5299 kcal/mol |
4.58402 kcal/mol |
8.54622 kcal/mol |
20 65@LB@ | |@LB@5'-UCC...CCACA C G AUUUCUC CU A UUAAA...CGG-3'@LB@ UGCUC U AUUAA CGUU CAUUGUC GCA@LB@ |:||| | ||:|| |:|| ||||||| |||@LB@ AUGAG A UAGUU GUAA GUAACAG CGU@LB@3'-AUU...AAAAA A G GUCCU CU G CGUUC...CAA-5'@LB@ | |@LB@ 187 154@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -22.5299 kcal/mol@LB@Unfolding Energy - mRNA 4.58402 kcal/mol@LB@Unfolding Energy - sRNA 8.54622 kcal/mol@LB@@LB@Position - mRNA 21 -- 64@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 139 -- 145@LB@ |
| 1 |
1.123e-05 |
0.04352 |
bsu13990 |
kinA |
-26.46 |
0.000103 |
182 -- 297 |
38 -- 181 |
sporulation kinase A |
bsu14490 bsu13530 |
_____________181___________________________________________________________________________________________________________________________298\n_____________|_____________________________________________________________________________________________________________________________|\n5'-CUA...GAGGG_____GUGG________________________________________________AA______________CAAAAACCGAUA___A_____________CUC____G____G____A_____AUAUC...ACU-3'\n______________AUUCU_____AACAGGAUA___________CGCAGCA_______________UGUUA____C_CA_CUUCAAA____________UUC__UGCAGUCUUGGC___UAAU_G_AC_CAUC_UUUAU\n______________|:|||_____|||||||:|___________|||||||_______________|||:|____|_||_|||||||____________|||__||||:|||||||___:|||_|_||_||||_:||||\n______________UGAGA_____UUGUCCUGU___________GCGUCGU_______________ACAGU____G_GU_GAAGUUU____________AAG__ACGUUAGAACCG___GUUA_C_UG_GUAG_GAAUA\n3'-AUU...AAAAA_____AGUAG_________AACUGUAACAG_______UCAAGGUAUAUUACA_____GAAA_A__A_______CAUUACUUACCG___CA____________UUU____G_A__G____A_____AGGGG...CAA-5'\n_____________|_____________________________________________________________________________________________________________________________|\n_____________182___________________________________________________________________________________________________________________________37\n |
259 -- 265 |
67 -- 73 |
-65.6544 kcal/mol |
17.7451 kcal/mol |
29.7341 kcal/mol |
181 298@LB@ | |@LB@5'-CUA...GAGGG GUGG AA CAAAAACCGAUA A CUC G G A AUAUC...ACU-3'@LB@ AUUCU AACAGGAUA CGCAGCA UGUUA C CA CUUCAAA UUC UGCAGUCUUGGC UAAU G AC CAUC UUUAU@LB@ |:||| |||||||:| ||||||| |||:| | || ||||||| ||| ||||:||||||| :||| | || |||| :||||@LB@ UGAGA UUGUCCUGU GCGUCGU ACAGU G GU GAAGUUU AAG ACGUUAGAACCG GUUA C UG GUAG GAAUA@LB@3'-AUU...AAAAA AGUAG AACUGUAACAG UCAAGGUAUAUUACA GAAA A A CAUUACUUACCG CA UUU G A G A AGGGG...CAA-5'@LB@ | |@LB@ 182 37@LB@@LB@@LB@Energy -26.46 kcal/mol@LB@Hybridization Energy -65.6544 kcal/mol@LB@Unfolding Energy - mRNA 17.7451 kcal/mol@LB@Unfolding Energy - sRNA 29.7341 kcal/mol@LB@@LB@Position - mRNA 182 -- 297@LB@Position - sRNA 38 -- 181@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 151 |
0.00683 |
0.1741 |
bsu18660 |
yoaM |
-13.14 |
0.080203 |
141 -- 183 |
39 -- 78 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu18880 bsu20490 |
_____________140________________________________184\n_____________|__________________________________|\n5'-UUU...UAAAU_______________CAAA__A____AAAA____UAAAA...AUA-3'\n______________GAACGAUCGU_CCCA____CU_CUUA____CCCC\n______________:|||:|||||_||||____||_||||____||||\n______________UUUGUUAGCA_GGGU____GA_GAAU____GGGG\n3'-AUU...AACCG__________U____A__________AA______UAAAG...CAA-5'\n_____________|__________________________________|\n_____________79_________________________________38\n |
154 -- 160 |
57 -- 63 |
-25.2857 kcal/mol |
3.90608 kcal/mol |
11.0213 kcal/mol |
140 184@LB@ | |@LB@5'-UUU...UAAAU CAAA A AAAA UAAAA...AUA-3'@LB@ GAACGAUCGU CCCA CU CUUA CCCC@LB@ :|||:||||| |||| || |||| ||||@LB@ UUUGUUAGCA GGGU GA GAAU GGGG@LB@3'-AUU...AACCG U A AA UAAAG...CAA-5'@LB@ | |@LB@ 79 38@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -25.2857 kcal/mol@LB@Unfolding Energy - mRNA 3.90608 kcal/mol@LB@Unfolding Energy - sRNA 11.0213 kcal/mol@LB@@LB@Position - mRNA 141 -- 183@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 57 -- 63@LB@ |
