| 1 |
1.123e-05 |
0.04352 |
bsu13990 |
kinA |
-26.46 |
0.000103 |
182 -- 297 |
38 -- 181 |
sporulation kinase A |
bsu14490 bsu13530 |
_____________181___________________________________________________________________________________________________________________________298\n_____________|_____________________________________________________________________________________________________________________________|\n5'-CUA...GAGGG_____GUGG________________________________________________AA______________CAAAAACCGAUA___A_____________CUC____G____G____A_____AUAUC...ACU-3'\n______________AUUCU_____AACAGGAUA___________CGCAGCA_______________UGUUA____C_CA_CUUCAAA____________UUC__UGCAGUCUUGGC___UAAU_G_AC_CAUC_UUUAU\n______________|:|||_____|||||||:|___________|||||||_______________|||:|____|_||_|||||||____________|||__||||:|||||||___:|||_|_||_||||_:||||\n______________UGAGA_____UUGUCCUGU___________GCGUCGU_______________ACAGU____G_GU_GAAGUUU____________AAG__ACGUUAGAACCG___GUUA_C_UG_GUAG_GAAUA\n3'-AUU...AAAAA_____AGUAG_________AACUGUAACAG_______UCAAGGUAUAUUACA_____GAAA_A__A_______CAUUACUUACCG___CA____________UUU____G_A__G____A_____AGGGG...CAA-5'\n_____________|_____________________________________________________________________________________________________________________________|\n_____________182___________________________________________________________________________________________________________________________37\n |
259 -- 265 |
67 -- 73 |
-65.6544 kcal/mol |
17.7451 kcal/mol |
29.7341 kcal/mol |
181 298@LB@ | |@LB@5'-CUA...GAGGG GUGG AA CAAAAACCGAUA A CUC G G A AUAUC...ACU-3'@LB@ AUUCU AACAGGAUA CGCAGCA UGUUA C CA CUUCAAA UUC UGCAGUCUUGGC UAAU G AC CAUC UUUAU@LB@ |:||| |||||||:| ||||||| |||:| | || ||||||| ||| ||||:||||||| :||| | || |||| :||||@LB@ UGAGA UUGUCCUGU GCGUCGU ACAGU G GU GAAGUUU AAG ACGUUAGAACCG GUUA C UG GUAG GAAUA@LB@3'-AUU...AAAAA AGUAG AACUGUAACAG UCAAGGUAUAUUACA GAAA A A CAUUACUUACCG CA UUU G A G A AGGGG...CAA-5'@LB@ | |@LB@ 182 37@LB@@LB@@LB@Energy -26.46 kcal/mol@LB@Hybridization Energy -65.6544 kcal/mol@LB@Unfolding Energy - mRNA 17.7451 kcal/mol@LB@Unfolding Energy - sRNA 29.7341 kcal/mol@LB@@LB@Position - mRNA 182 -- 297@LB@Position - sRNA 38 -- 181@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 2 |
6.323e-05 |
0.08823 |
bsu13370 |
ykoQ |
-21.23 |
0.001523 |
8 -- 146 |
40 -- 170 |
metallophosphoesterase |
|
_____________7_______________________________________________________________________________________________________________________________________________________147\n_____________|_______________________________________________________________________________________________________________________________________________________|\n____5'-CGUAACA______ACA____C__UUGU___UC____________U_____U_____GCCUC_________AAA_______A__CCUUUUCC__________CAAAU__U__AUCAUAU______C___UUUUCA__________ACCCAUUA______GCUAU...CAA-3'\n______________UUGCUU___CAUC_AU____UAU__UGAC____UCCG_CGGCA_GUUCU_____AUG_UGUCA____UUUAUU_UC______________CUUC_____CA_UC_______GACAAU_CGU______ACCUGUCUCU________AUUCCC\n______________||:||:___||||_|:____:||__||||____||||_|:|||_||||:_____|||_|||||____:|:||:_||______________||||_____||_||_______:|||||_|||______|||::|||||________||||||\n______________AAUGAG___GUAG_UG____GUA__ACUG____AGGC_GUCGU_CAAGG_____UAC_ACAGU____GAGUAG_AG______________GAAG_____GU_AG_______UUGUUA_GCA______UGGGUAGAGA________UAAGGG\n3'-AUU...AAAAA______AA_____U__UCCU_________UAAC__________U_____UAUAU___A_____GAAA______A__UUUCAUUACUUACC____CAAC___U__AACCGU___________________________A_____________GUAAA...CAA-5'\n_____________|_______________________________________________________________________________________________________________________________________________________|\n_____________171_____________________________________________________________________________________________________________________________________________________39\n |
126 -- 132 |
47 -- 53 |
-61.8379 kcal/mol |
13.5948 kcal/mol |
30.7766 kcal/mol |
7 147@LB@ | |@LB@ 5'-CGUAACA ACA C UUGU UC U U GCCUC AAA A CCUUUUCC CAAAU U AUCAUAU C UUUUCA ACCCAUUA GCUAU...CAA-3'@LB@ UUGCUU CAUC AU UAU UGAC UCCG CGGCA GUUCU AUG UGUCA UUUAUU UC CUUC CA UC GACAAU CGU ACCUGUCUCU AUUCCC@LB@ ||:||: |||| |: :|| |||| |||| |:||| ||||: ||| ||||| :|:||: || |||| || || :||||| ||| |||::||||| ||||||@LB@ AAUGAG GUAG UG GUA ACUG AGGC GUCGU CAAGG UAC ACAGU GAGUAG AG GAAG GU AG UUGUUA GCA UGGGUAGAGA UAAGGG@LB@3'-AUU...AAAAA AA U UCCU UAAC U UAUAU A GAAA A UUUCAUUACUUACC CAAC U AACCGU A GUAAA...CAA-5'@LB@ | |@LB@ 171 39@LB@@LB@@LB@Energy -21.23 kcal/mol@LB@Hybridization Energy -61.8379 kcal/mol@LB@Unfolding Energy - mRNA 13.5948 kcal/mol@LB@Unfolding Energy - sRNA 30.7766 kcal/mol@LB@@LB@Position - mRNA 8 -- 146@LB@Position - sRNA 40 -- 170@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 3 |
8.73e-05 |
0.08823 |
bsu25090 |
yqfW |
-16.63 |
0.015052 |
29 -- 79 |
33 -- 81 |
nucleotidase YqfW |
|
_____________28____________________________________________________80\n_____________|_____________________________________________________|\n5'-CCU...GUAAC______U___AC___UUUUCG_______UUG___C___CA_______C_____GUUUU...ACG-3'\n______________UGUUGU_AUC___GC______UAAUCGU___CCA_CUU__AUUCCCC__UUCU\n______________:||||:_|||___||______:||||||___|||_||:__|||||||__||||\n______________GCAACG_UAG___CG______GUUAGCA___GGU_GAG__UAAGGGG__AAGA\n3'-AUU...CCGAA______U___AAC__UUU__________UG____A___AA_______UA____AACUU...CAA-5'\n_____________|_____________________________________________________|\n_____________82____________________________________________________32\n |
68 -- 74 |
39 -- 45 |
-32.9438 kcal/mol |
5.67451 kcal/mol |
13.9362 kcal/mol |
28 80@LB@ | |@LB@5'-CCU...GUAAC U AC UUUUCG UUG C CA C GUUUU...ACG-3'@LB@ UGUUGU AUC GC UAAUCGU CCA CUU AUUCCCC UUCU@LB@ :||||: ||| || :|||||| ||| ||: ||||||| ||||@LB@ GCAACG UAG CG GUUAGCA GGU GAG UAAGGGG AAGA@LB@3'-AUU...CCGAA U AAC UUU UG A AA UA AACUU...CAA-5'@LB@ | |@LB@ 82 32@LB@@LB@@LB@Energy -16.63 kcal/mol@LB@Hybridization Energy -32.9438 kcal/mol@LB@Unfolding Energy - mRNA 5.67451 kcal/mol@LB@Unfolding Energy - sRNA 13.9362 kcal/mol@LB@@LB@Position - mRNA 29 -- 79@LB@Position - sRNA 33 -- 81@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 4 |
0.0001192 |
0.08823 |
bsu12150 |
yjgB |
-21.60 |
0.001260 |
37 -- 72 |
32 -- 79 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 1580552 |
|
_____________36_______________________________________________73\n_____________|________________________________________________|\n5'-CCU...UGUGA____UU_____C____________________C_______________GCUUU...CAG-3'\n______________UUGU__UCUUG_CAG__________CCAUCUC__AUUCCCC_UUUCUU\n______________|||:__|||||_||:__________|||||||__|||||||_||||||\n______________AACG__AGAAC_GUU__________GGUAGAG__UAAGGGG_AAAGAA\n3'-AUU...GAAGC____UU_____C___UGUUAGCAUG_______AA_______U______ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________80_______________________________________________31\n |
60 -- 66 |
39 -- 45 |
-34.5565 kcal/mol |
2.03428 kcal/mol |
13.9015 kcal/mol |
36 73@LB@ | |@LB@5'-CCU...UGUGA UU C C GCUUU...CAG-3'@LB@ UUGU UCUUG CAG CCAUCUC AUUCCCC UUUCUU@LB@ |||: ||||| ||: ||||||| ||||||| ||||||@LB@ AACG AGAAC GUU GGUAGAG UAAGGGG AAAGAA@LB@3'-AUU...GAAGC UU C UGUUAGCAUG AA U ACUUU...CAA-5'@LB@ | |@LB@ 80 31@LB@@LB@@LB@Energy -21.60 kcal/mol@LB@Hybridization Energy -34.5565 kcal/mol@LB@Unfolding Energy - mRNA 2.03428 kcal/mol@LB@Unfolding Energy - sRNA 13.9015 kcal/mol@LB@@LB@Position - mRNA 37 -- 72@LB@Position - sRNA 32 -- 79@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 5 |
0.0001198 |
0.08823 |
bsu33680 |
yvaP |
-23.88 |
0.000393 |
26 -- 134 |
34 -- 167 |
HTH-type transcriptional regulator YvaP |
|
_____________25___________________________________________________________________________________________________________________________________________________________135\n_____________|____________________________________________________________________________________________________________________________________________________________|\n5'-UUU...AACAU_______A______________AAA____________AUA________________A_______________AGCAAAAAAAC__________C_____C__UC______C___________CU____A______AUCAAUC______________CAUGC...CGC-3'\n______________CUC_UCA__AGCAGGAUAUUGA_____UCU_CAGCAA___CU___________UCG_UUUUUUGUCUUCAGA___________GC______GC__UCUU__C__AUAAUC___CCUGUUUCU__UUCU_UAUUUU______________ACGCUUG\n______________|||_|||__|:|||||:|||||_____||:_||||||___|:___________||:_|||:|::||||||:|___________||______||__||||__|__|:||||___||::|:|||__|||:_:||||:______________||||||:\n______________GAG_AGU__UUGUCCUGUAACU_____AGG_GUCGUU___GG___________AGU_AAAGAGUAGAAGUUU___________CG______CG__AGAA__G__UGUUAG___GGGUAGAGA__AAGG_GUAAAG______________UGCGAAU\n3'-AUU...AAAAU___A___AG_____________GUAAC___C______CAA__UAUAUUACAAC___G_______________CAUUACUUAC___AAGCAA__UU____CC_UU______CAU_________AU____G______AAACUUUAAACUUU_______UAAAG...CAA-5'\n_____________|____________________________________________________________________________________________________________________________________________________________|\n_____________168__________________________________________________________________________________________________________________________________________________________33\n |
142 -- 148 |
13 -- 19 |
-70.4657 kcal/mol |
17.0109 kcal/mol |
33.4152 kcal/mol |
25 135@LB@ | |@LB@5'-UUU...AACAU A AAA AUA A AGCAAAAAAAC C C UC C CU A AUCAAUC CAUGC...CGC-3'@LB@ CUC UCA AGCAGGAUAUUGA UCU CAGCAA CU UCG UUUUUUGUCUUCAGA GC GC UCUU C AUAAUC CCUGUUUCU UUCU UAUUUU ACGCUUG@LB@ ||| ||| |:|||||:||||| ||: |||||| |: ||: |||:|::||||||:| || || |||| | |:|||| ||::|:||| |||: :||||: ||||||:@LB@ GAG AGU UUGUCCUGUAACU AGG GUCGUU GG AGU AAAGAGUAGAAGUUU CG CG AGAA G UGUUAG GGGUAGAGA AAGG GUAAAG UGCGAAU@LB@3'-AUU...AAAAU A AG GUAAC C CAA UAUAUUACAAC G CAUUACUUAC AAGCAA UU CC UU CAU AU G AAACUUUAAACUUU UAAAG...CAA-5'@LB@ | |@LB@ 168 33@LB@@LB@@LB@Energy -23.88 kcal/mol@LB@Hybridization Energy -70.4657 kcal/mol@LB@Unfolding Energy - mRNA 17.0109 kcal/mol@LB@Unfolding Energy - sRNA 33.4152 kcal/mol@LB@@LB@Position - mRNA 26 -- 134@LB@Position - sRNA 34 -- 167@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 6 |
0.0001543 |
0.08823 |
bsu10740 |
yisJ |
-16.00 |
0.020473 |
36 -- 139 |
34 -- 134 |
inner spore coat protein H-like protein |
|
_____________35____________________________________________________140\n_____________|_____________________________________________________|\n5'-GGC...GUUCC______GCAAAUUUCAUG_______U____CA_____U____C__________CCUUC...UUC-3'\n______________CCUGUC____________UUAUUCC_CCAU__UCUUU_AAAU___AGAAUGCU\n______________||::||____________|||||||_||||__|||||_||||___|:||:|||\n______________GGGUAG____________AAUAAGG_GGUA__AGAAA_UUUA___UUUUGCGA\n3'-AUU...AGCAU______AG______________________A______C____AAC________AUUAA...CAA-5'\n_____________|_____________________________________________________|\n_____________135___________________________________________________33\n |
74 -- 80 |
15 -- 21 |
-28.1573 kcal/mol |
3.71522 kcal/mol |
11.7008 kcal/mol |
35 140@LB@ | |@LB@5'-GGC...GUUCC GCAAAUUUCAUG U CA U C CCUUC...UUC-3'@LB@ CCUGUC UUAUUCC CCAU UCUUU AAAU AGAAUGCU@LB@ ||::|| ||||||| |||| ||||| |||| |:||:|||@LB@ GGGUAG AAUAAGG GGUA AGAAA UUUA UUUUGCGA@LB@3'-AUU...AGCAU AG A C AAC AUUAA...CAA-5'@LB@ | |@LB@ 135 33@LB@@LB@@LB@Energy -16.00 kcal/mol@LB@Hybridization Energy -28.1573 kcal/mol@LB@Unfolding Energy - mRNA 3.71522 kcal/mol@LB@Unfolding Energy - sRNA 11.7008 kcal/mol@LB@@LB@Position - mRNA 36 -- 139@LB@Position - sRNA 34 -- 134@LB@Position Seed - mRNA 74 -- 80@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 7 |
0.0001858 |
0.08823 |
bsu34210 |
yvfG |
-17.07 |
0.012126 |
85 -- 142 |
32 -- 86 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________84____________________________________________________________143\n_____________|_____________________________________________________________|\n5'-GAG...GUUGA______AAUAC___________C____GUU__A__________GCUCAC____________CAUUG...ACU-3'\n______________GGCUUU_____UUGUA__CUUG_UGAA___UC_UAUCCAUCUU______UCCCC_UUUCUU\n______________|||||:_____|||:|__||||_::||___||_||:||||||:______|||||_||||||\n______________CCGAAG_____AACGU__GAAC_GUUU___AG_AUGGGUAGAG______AGGGG_AAAGAA\n3'-AUU...ACUUA______C_________UA____C____GUU__C__________AAUA_______U______ACUUU...CAA-5'\n_____________|_____________________________________________________________|\n_____________87____________________________________________________________31\n |
119 -- 125 |
48 -- 54 |
-40.7387 kcal/mol |
11.2598 kcal/mol |
14.8836 kcal/mol |
84 143@LB@ | |@LB@5'-GAG...GUUGA AAUAC C GUU A GCUCAC CAUUG...ACU-3'@LB@ GGCUUU UUGUA CUUG UGAA UC UAUCCAUCUU UCCCC UUUCUU@LB@ |||||: |||:| |||| ::|| || ||:||||||: ||||| ||||||@LB@ CCGAAG AACGU GAAC GUUU AG AUGGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACUUA C UA C GUU C AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 87 31@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -40.7387 kcal/mol@LB@Unfolding Energy - mRNA 11.2598 kcal/mol@LB@Unfolding Energy - sRNA 14.8836 kcal/mol@LB@@LB@Position - mRNA 85 -- 142@LB@Position - sRNA 32 -- 86@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 8 |
0.0001871 |
0.08823 |
bsu22860 |
yphA |
-19.72 |
0.003251 |
3 -- 123 |
37 -- 178 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________2_______________________________________________________________________________________________________124\n_____________|_______________________________________________________________________________________________________|\n5'-UCG...AAGGC________CCG______________________AC_G__C_______GAUUC______________AAGCUUUGAGUUUUU________CUGUCUU_______GAUCA...UUA-3'\n______________UUGUCGCU___UCA_________UAGUGAAUGG__U_GU_GCGAUCU_____GAC__UC_UGCCUG_______________UCUCUUGU_______UUCCCAU\n______________|||||:||___|||_________||:|||||||__|_||_||:||||_____:||__||_|:||::_______________||||||:|_______|:|||||\n______________AACAGUGA___AGU_________AUUACUUACC__A_CA_CGUUAGA_____UUG__AG_AUGGGU_______________AGAGAAUA_______AGGGGUA\n3'-AUU...AUUAC________AAG___AGAAGUUUC__________GA_G__A_______ACCGU___UU__C___________________________________________AAGAA...CAA-5'\n_____________|_______________________________________________________________________________________________________|\n_____________179_____________________________________________________________________________________________________36\n |
117 -- 123 |
37 -- 43 |
-53.1639 kcal/mol |
19.1133 kcal/mol |
22.4377 kcal/mol |
2 124@LB@ | |@LB@5'-UCG...AAGGC CCG AC G C GAUUC AAGCUUUGAGUUUUU CUGUCUU GAUCA...UUA-3'@LB@ UUGUCGCU UCA UAGUGAAUGG U GU GCGAUCU GAC UC UGCCUG UCUCUUGU UUCCCAU@LB@ |||||:|| ||| ||:||||||| | || ||:|||| :|| || |:||:: ||||||:| |:|||||@LB@ AACAGUGA AGU AUUACUUACC A CA CGUUAGA UUG AG AUGGGU AGAGAAUA AGGGGUA@LB@3'-AUU...AUUAC AAG AGAAGUUUC GA G A ACCGU UU C AAGAA...CAA-5'@LB@ | |@LB@ 179 36@LB@@LB@@LB@Energy -19.72 kcal/mol@LB@Hybridization Energy -53.1639 kcal/mol@LB@Unfolding Energy - mRNA 19.1133 kcal/mol@LB@Unfolding Energy - sRNA 22.4377 kcal/mol@LB@@LB@Position - mRNA 3 -- 123@LB@Position - sRNA 37 -- 178@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 9 |
0.0002071 |
0.08823 |
bsu08425 |
mprF |
-16.98 |
0.012672 |
3 -- 50 |
34 -- 81 |
phosphatidylglycerol lysyltransferase |
|
_____________2_______________________________________________51\n_____________|_______________________________________________|\n_________5'-AC__________GU_______U__AU__________AUCAC________CAUUC...AUC-3'\n______________UGUUGU_AUC___GUAAAU_AU____UCCAUUUC_____UUCCUCAU\n______________:||||:_|||___|:|||:_||____:||||:||_____||||:|||\n______________GCAACG_UAG___CGUUUG_UA____GGGUAGAG_____AAGGGGUA\n3'-AUU...CCGAA______U___AAC______U__GCAU________AAU__________AAGAA...CAA-5'\n_____________|_______________________________________________|\n_____________82______________________________________________33\n |
39 -- 45 |
37 -- 43 |
-29.0114 kcal/mol |
1.80779 kcal/mol |
13.626 kcal/mol |
2 51@LB@ | |@LB@ 5'-AC GU U AU AUCAC CAUUC...AUC-3'@LB@ UGUUGU AUC GUAAAU AU UCCAUUUC UUCCUCAU@LB@ :||||: ||| |:|||: || :||||:|| ||||:|||@LB@ GCAACG UAG CGUUUG UA GGGUAGAG AAGGGGUA@LB@3'-AUU...CCGAA U AAC U GCAU AAU AAGAA...CAA-5'@LB@ | |@LB@ 82 33@LB@@LB@@LB@Energy -16.98 kcal/mol@LB@Hybridization Energy -29.0114 kcal/mol@LB@Unfolding Energy - mRNA 1.80779 kcal/mol@LB@Unfolding Energy - sRNA 13.626 kcal/mol@LB@@LB@Position - mRNA 3 -- 50@LB@Position - sRNA 34 -- 81@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 10 |
0.0002277 |
0.08823 |
bsu05690 |
ydhB |
-21.23 |
0.001523 |
113 -- 150 |
136 -- 182 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
bsu12400 |
_____________112_______________________________151\n_____________|_________________________________|\n5'-CAU...UGUUU__________________C______ACC_____CAGAA...UCC-3'\n______________UUCUUCGUCAAUAGGACA____CAU___UCCGC\n______________:|||||:||||:||||||____|||___|||||\n______________GAGAAGUAGUUGUCCUGU____GUA___AGGCG\n3'-AUU...AAAAU__________________AACU___AC______UCGUU...CAA-5'\n_____________|_________________________________|\n_____________183_______________________________135\n |
132 -- 138 |
150 -- 156 |
-33.3473 kcal/mol |
6.29637 kcal/mol |
7.50175 kcal/mol |
112 151@LB@ | |@LB@5'-CAU...UGUUU C ACC CAGAA...UCC-3'@LB@ UUCUUCGUCAAUAGGACA CAU UCCGC@LB@ :|||||:||||:|||||| ||| |||||@LB@ GAGAAGUAGUUGUCCUGU GUA AGGCG@LB@3'-AUU...AAAAU AACU AC UCGUU...CAA-5'@LB@ | |@LB@ 183 135@LB@@LB@@LB@Energy -21.23 kcal/mol@LB@Hybridization Energy -33.3473 kcal/mol@LB@Unfolding Energy - mRNA 6.29637 kcal/mol@LB@Unfolding Energy - sRNA 7.50175 kcal/mol@LB@@LB@Position - mRNA 113 -- 150@LB@Position - sRNA 136 -- 182@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 150 -- 156@LB@ |
