| 68 |
0.002291 |
0.1242 |
bsu02600 |
cwlJ |
-9.38 |
0.396883 |
135 -- 166 |
88 -- 116 |
cell wall hydrolase CwlJ |
|
_____________134______________________________167\n_____________|________________________________|\n5'-UGU...UCUCC____C__CCG_____AC___U___________AUAUG...UCG-3'\n______________UGUC_CU___UCAUC__UUC_GAAGUAAUGAA\n______________||||_||___|||||__|||_:||||||||||\n______________ACAG_GA___AGUAG__AAG_UUUCAUUACUU\n3'-AUU...UUACA____U__AAG______________________ACCGA...CAA-5'\n_____________|________________________________|\n_____________117______________________________87\n |
159 -- 165 |
89 -- 95 |
-22.7508 kcal/mol |
5.74597 kcal/mol |
10.169 kcal/mol |
134 167@LB@ | |@LB@5'-UGU...UCUCC C CCG AC U AUAUG...UCG-3'@LB@ UGUC CU UCAUC UUC GAAGUAAUGAA@LB@ |||| || ||||| ||| :||||||||||@LB@ ACAG GA AGUAG AAG UUUCAUUACUU@LB@3'-AUU...UUACA U AAG ACCGA...CAA-5'@LB@ | |@LB@ 117 87@LB@@LB@@LB@Energy -9.38 kcal/mol@LB@Hybridization Energy -22.7508 kcal/mol@LB@Unfolding Energy - mRNA 5.74597 kcal/mol@LB@Unfolding Energy - sRNA 10.169 kcal/mol@LB@@LB@Position - mRNA 135 -- 166@LB@Position - sRNA 88 -- 116@LB@Position Seed - mRNA 159 -- 165@LB@Position Seed - sRNA 89 -- 95@LB@ |
| 180 |
0.008222 |
0.1751 |
bsu14560 |
def |
-9.11 |
0.436669 |
135 -- 145 |
75 -- 85 |
peptide deformylase |
|
_____________134_________146\n_____________|___________|\n5'-UGG...CAUUC___________CCUUU...AGC-3'\n______________GUUUCGUUGCA\n______________|:|||||||||\n______________CGAAGCAACGU\n3'-AUU...CUUAC___________UAGAA...CAA-5'\n_____________|___________|\n_____________86__________74\n |
139 -- 145 |
75 -- 81 |
-16.022 kcal/mol |
4.21993 kcal/mol |
4.54464 kcal/mol |
134 146@LB@ | |@LB@5'-UGG...CAUUC CCUUU...AGC-3'@LB@ GUUUCGUUGCA@LB@ |:|||||||||@LB@ CGAAGCAACGU@LB@3'-AUU...CUUAC UAGAA...CAA-5'@LB@ | |@LB@ 86 74@LB@@LB@@LB@Energy -9.11 kcal/mol@LB@Hybridization Energy -16.022 kcal/mol@LB@Unfolding Energy - mRNA 4.21993 kcal/mol@LB@Unfolding Energy - sRNA 4.54464 kcal/mol@LB@@LB@Position - mRNA 135 -- 145@LB@Position - sRNA 75 -- 85@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 75 -- 81@LB@ |
| 12 |
0.0004273 |
0.1011 |
bsu13640 |
spo0E |
-21.66 |
0.001221 |
136 -- 180 |
32 -- 78 |
aspartyl-phosphate phosphatase Spo0E |
|
_____________135________________________________________________181\n_____________|__________________________________________________|\n5'-GGC...UAAUC______AAU__________A______A___AAA_________________AGCGG...AUG-3'\n______________UGUAAU____GC__ACAAU___ACCC_AUC___CUUGUUUCCUAUUUCUU\n______________||:|||____||__|||||___||||_|||___|||:||:||:|||||||\n______________ACGUUA____CG__UGUUA___UGGG_UAG___GAAUAAGGGGUAAAGAA\n3'-AUU...AAGCA______GAAC__UU_____GCA________A___________________ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________79_________________________________________________31\n |
172 -- 178 |
34 -- 40 |
-36.2602 kcal/mol |
3.78665 kcal/mol |
13.8985 kcal/mol |
135 181@LB@ | |@LB@5'-GGC...UAAUC AAU A A AAA AGCGG...AUG-3'@LB@ UGUAAU GC ACAAU ACCC AUC CUUGUUUCCUAUUUCUU@LB@ ||:||| || ||||| |||| ||| |||:||:||:|||||||@LB@ ACGUUA CG UGUUA UGGG UAG GAAUAAGGGGUAAAGAA@LB@3'-AUU...AAGCA GAAC UU GCA A ACUUU...CAA-5'@LB@ | |@LB@ 79 31@LB@@LB@@LB@Energy -21.66 kcal/mol@LB@Hybridization Energy -36.2602 kcal/mol@LB@Unfolding Energy - mRNA 3.78665 kcal/mol@LB@Unfolding Energy - sRNA 13.8985 kcal/mol@LB@@LB@Position - mRNA 136 -- 180@LB@Position - sRNA 32 -- 78@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 104 |
0.004338 |
0.1606 |
bsu08000 |
yfjQ |
-9.77 |
0.342120 |
136 -- 162 |
103 -- 137 |
metal ion transporter YfjQ |
bsu24740 |
_____________135_______________163\n_____________|_________________|\n5'-CAU...AUUAG____A____A_______CUUUU...AUU-3'\n______________GCAG_GAGU_CCGUAUA\n______________||||_:|||_||:||||\n______________CGUC_UUCA_GGUAUAU\n3'-AUU...ACAGG____G____A_______UACAA...CAA-5'\n_____________|_________________|\n_____________138_______________102\n |
146 -- 152 |
121 -- 127 |
-16.4629 kcal/mol |
4.99509 kcal/mol |
3.33887 kcal/mol |
135 163@LB@ | |@LB@5'-CAU...AUUAG A A CUUUU...AUU-3'@LB@ GCAG GAGU CCGUAUA@LB@ |||| :||| ||:||||@LB@ CGUC UUCA GGUAUAU@LB@3'-AUU...ACAGG G A UACAA...CAA-5'@LB@ | |@LB@ 138 102@LB@@LB@@LB@Energy -9.77 kcal/mol@LB@Hybridization Energy -16.4629 kcal/mol@LB@Unfolding Energy - mRNA 4.99509 kcal/mol@LB@Unfolding Energy - sRNA 3.33887 kcal/mol@LB@@LB@Position - mRNA 136 -- 162@LB@Position - sRNA 103 -- 137@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 80 |
0.002732 |
0.1323 |
bsu14790 |
ylaI |
-9.42 |
0.391167 |
138 -- 189 |
118 -- 178 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________137____________________________________________________190\n_____________|______________________________________________________|\n5'-AGA...CUUUA_______C___UUUU_____GCUUC____A_________UAAUA____U_____AAAGA...AUA-3'\n______________GCUUUUU_UUA____GGACA_______UG_UCCGUGGUA_____CAUG_UAGUG\n______________:||:||:_|:|____|||||_______||_||||::|:|_____|||:_||:||\n______________UGAGAAG_AGU____CCUGU_______AC_AGGCGUCGU_____GUAU_AUUAC\n3'-AUU...AAAAA_______U___UGU______AACUGUA____________UCAAG__________AACAG...CAA-5'\n_____________|______________________________________________________|\n_____________179____________________________________________________117\n |
167 -- 173 |
133 -- 139 |
-31.618 kcal/mol |
11.7219 kcal/mol |
10.6253 kcal/mol |
137 190@LB@ | |@LB@5'-AGA...CUUUA C UUUU GCUUC A UAAUA U AAAGA...AUA-3'@LB@ GCUUUUU UUA GGACA UG UCCGUGGUA CAUG UAGUG@LB@ :||:||: |:| ||||| || ||||::|:| |||: ||:||@LB@ UGAGAAG AGU CCUGU AC AGGCGUCGU GUAU AUUAC@LB@3'-AUU...AAAAA U UGU AACUGUA UCAAG AACAG...CAA-5'@LB@ | |@LB@ 179 117@LB@@LB@@LB@Energy -9.42 kcal/mol@LB@Hybridization Energy -31.618 kcal/mol@LB@Unfolding Energy - mRNA 11.7219 kcal/mol@LB@Unfolding Energy - sRNA 10.6253 kcal/mol@LB@@LB@Position - mRNA 138 -- 189@LB@Position - sRNA 118 -- 178@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 133 -- 139@LB@ |
| 153 |
0.007014 |
0.1751 |
bsu10400 |
yhxC |
-16.66 |
0.014878 |
138 -- 167 |
138 -- 167 |
oxidoreductase |
|
_____________137____________________________168\n_____________|______________________________|\n5'-UCC...UCUAU________AC______AGCA__________AUAAC...CGA-3'\n______________CUUUUCAU__AUAGGA____GGCAUUGUUC\n______________||:|||||__|:||||____|:||||||:|\n______________GAGAAGUA__UGUCCU____CUGUAACAGG\n3'-AUU...AAAAU________GU______GUAA__________CGUCG...CAA-5'\n_____________|______________________________|\n_____________168____________________________137\n |
161 -- 167 |
138 -- 144 |
-29.0079 kcal/mol |
5.02609 kcal/mol |
7.37082 kcal/mol |
137 168@LB@ | |@LB@5'-UCC...UCUAU AC AGCA AUAAC...CGA-3'@LB@ CUUUUCAU AUAGGA GGCAUUGUUC@LB@ ||:||||| |:|||| |:||||||:|@LB@ GAGAAGUA UGUCCU CUGUAACAGG@LB@3'-AUU...AAAAU GU GUAA CGUCG...CAA-5'@LB@ | |@LB@ 168 137@LB@@LB@@LB@Energy -16.66 kcal/mol@LB@Hybridization Energy -29.0079 kcal/mol@LB@Unfolding Energy - mRNA 5.02609 kcal/mol@LB@Unfolding Energy - sRNA 7.37082 kcal/mol@LB@@LB@Position - mRNA 138 -- 167@LB@Position - sRNA 138 -- 167@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 138 -- 144@LB@ |
| 154 |
0.00707 |
0.1751 |
bsu07830 |
yfkO |
-12.90 |
0.089530 |
138 -- 152 |
158 -- 172 |
NAD(P)H nitroreductase YfkO |
|
_____________137____________153\n_____________|______________|\n5'-GGA...GUACU______________UAAUG...AAU-3'\n______________UUUACUUUUUAUCA\n______________||||||:||:||||\n______________AAAUGAGAAGUAGU\n3'-AUU...AAAAA______________UGUCC...CAA-5'\n_____________|______________|\n_____________173____________157\n |
146 -- 152 |
158 -- 164 |
-13.59 kcal/mol |
1.38023 kcal/mol |
1.02713 kcal/mol |
137 153@LB@ | |@LB@5'-GGA...GUACU UAAUG...AAU-3'@LB@ UUUACUUUUUAUCA@LB@ ||||||:||:||||@LB@ AAAUGAGAAGUAGU@LB@3'-AUU...AAAAA UGUCC...CAA-5'@LB@ | |@LB@ 173 157@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -13.59 kcal/mol@LB@Unfolding Energy - mRNA 1.38023 kcal/mol@LB@Unfolding Energy - sRNA 1.02713 kcal/mol@LB@@LB@Position - mRNA 138 -- 152@LB@Position - sRNA 158 -- 172@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 110 |
0.004746 |
0.1671 |
bsu09940 |
yhaL |
-13.90 |
0.056226 |
141 -- 159 |
37 -- 56 |
sporulation protein YhaL |
|
_____________140______________________________________160\n_____________|________________________________________|\n5'-GAA...UUUUU____________GUA___U____U___A_GCA________AGCGC...ACC-3'\n______________UCCGUGGUGGGU___UCU_UGUA_UGU_G___UCAUUUUC\n______________||||::|:::||___||:_|:||_|||_|___|||:||||\n______________AGGCGUCGUUCA___AGG_AUAU_ACA_C___AGUGAAAG\n3'-AUU...GUAAC__________________U____U___A____________AGUAG...CAA-5'\n_____________|________________________________________|\n_____________57_______________________________________36\n |
245 -- 251 |
107 -- 113 |
-27.9954 kcal/mol |
7.44184 kcal/mol |
9.97721 kcal/mol |
140 160@LB@ | |@LB@5'-GAA...UUUUU GUA U U A GCA AGCGC...ACC-3'@LB@ UCCGUGGUGGGU UCU UGUA UGU G UCAUUUUC@LB@ ||||::|:::|| ||: |:|| ||| | |||:||||@LB@ AGGCGUCGUUCA AGG AUAU ACA C AGUGAAAG@LB@3'-AUU...GUAAC U U A AGUAG...CAA-5'@LB@ | |@LB@ 57 36@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -27.9954 kcal/mol@LB@Unfolding Energy - mRNA 7.44184 kcal/mol@LB@Unfolding Energy - sRNA 9.97721 kcal/mol@LB@@LB@Position - mRNA 141 -- 159@LB@Position - sRNA 37 -- 56@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 151 |
0.00683 |
0.1741 |
bsu18660 |
yoaM |
-13.14 |
0.080203 |
141 -- 183 |
39 -- 78 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu18880 bsu20490 |
_____________140________________________________184\n_____________|__________________________________|\n5'-UUU...UAAAU_______________CAAA__A____AAAA____UAAAA...AUA-3'\n______________GAACGAUCGU_CCCA____CU_CUUA____CCCC\n______________:|||:|||||_||||____||_||||____||||\n______________UUUGUUAGCA_GGGU____GA_GAAU____GGGG\n3'-AUU...AACCG__________U____A__________AA______UAAAG...CAA-5'\n_____________|__________________________________|\n_____________79_________________________________38\n |
154 -- 160 |
57 -- 63 |
-25.2857 kcal/mol |
3.90608 kcal/mol |
11.0213 kcal/mol |
140 184@LB@ | |@LB@5'-UUU...UAAAU CAAA A AAAA UAAAA...AUA-3'@LB@ GAACGAUCGU CCCA CU CUUA CCCC@LB@ :|||:||||| |||| || |||| ||||@LB@ UUUGUUAGCA GGGU GA GAAU GGGG@LB@3'-AUU...AACCG U A AA UAAAG...CAA-5'@LB@ | |@LB@ 79 38@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -25.2857 kcal/mol@LB@Unfolding Energy - mRNA 3.90608 kcal/mol@LB@Unfolding Energy - sRNA 11.0213 kcal/mol@LB@@LB@Position - mRNA 141 -- 183@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 57 -- 63@LB@ |
| 187 |
0.008608 |
0.1751 |
bsu17960 |
yneK |
-9.96 |
0.317804 |
143 -- 172 |
155 -- 184 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________142___________173\n_____________|_____________|\n5'-AGU...AAUCU________AU___AUAUG...UAA-3'\n______________CUCUUCGU__ACA\n______________||||||:|__|||\n______________GAGAAGUA__UGU\n3'-AUU...AAAAU________GU___CCUGU...CAA-5'\n_____________|_____________|\n_____________185___________154\n |
161 -- 167 |
160 -- 166 |
-12.096 kcal/mol |
1.39305 kcal/mol |
1.41024 kcal/mol |
142 173@LB@ | |@LB@5'-AGU...AAUCU AU AUAUG...UAA-3'@LB@ CUCUUCGU ACA@LB@ ||||||:| |||@LB@ GAGAAGUA UGU@LB@3'-AUU...AAAAU GU CCUGU...CAA-5'@LB@ | |@LB@ 185 154@LB@@LB@@LB@Energy -9.96 kcal/mol@LB@Hybridization Energy -12.096 kcal/mol@LB@Unfolding Energy - mRNA 1.39305 kcal/mol@LB@Unfolding Energy - sRNA 1.41024 kcal/mol@LB@@LB@Position - mRNA 143 -- 172@LB@Position - sRNA 155 -- 184@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 160 -- 166@LB@ |
