Freiburg RNA Tools
SECISDesign - Insert SECIS in mRNA
BIF
IFF
SECISDesign

SECISDesign - Insert SECIS in mRNA

SECISDesign is a server for the design of SECIS-elements within the coding sequence of an mRNA with both structure and sequence constraints. This will trigger the insertion of a selenocystein at a preceding STOP codon. Furthermore, a certain similarity to the original protein sequence is kept. It can be used e.g. for recombinant expression of selenoproteins in E.coli.

Note: SECISDesign is not maintained anymore.

For articles describing the tool refer to the reference section below. Please cite us when using our tools.
For more information check the help page.

Try SECISDesign with these examples:

Note: the SECISDesign webserver will be offline for maintenance from 25/07/2017 to 26/07/2017.

Due to construction work at the computing center of the University of Freiburg, we have to temporarily stop the Freiburg RNA tools service. We will restart the service as soon as possible.

We apologize for the inconvenience.

Original Protein Sequence

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SECIS Design Constraints

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FdhF-std FdhF-std FdhF-insert FdhF-insert FdhF-delete FdhF-delete
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FdhF-std FdhF-std+optional FdhF-insert FdhF-insert+optional FdhF-delete FdhF-delete+optional
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When using SECISDesign please cite :

Results are computed with SECISDesign version 1.0 (2009-10-13) using Turner99 energies