| 176 |
0.008122 |
0.1751 |
bsu16520 |
frr |
-16.32 |
0.017549 |
85 -- 113 |
136 -- 167 |
ribosome-recycling factor |
|
_____________84________________________________114\n_____________|_________________________________|\n5'-AGA...GCUUC_____________U_____A_____________UUAUC...AUC-3'\n______________CUCUUUAU__GCA_GGACA_UGACAU__UCCGC\n______________|||||:||__:||_|||||_||||||__|||||\n______________GAGAAGUA__UGU_CCUGU_ACUGUA__AGGCG\n3'-AUU...AAAAU________GU_________A______AC_____UCGUU...CAA-5'\n_____________|_________________________________|\n_____________168_______________________________135\n |
86 -- 92 |
160 -- 166 |
-32.0013 kcal/mol |
8.50527 kcal/mol |
7.50175 kcal/mol |
84 114@LB@ | |@LB@5'-AGA...GCUUC U A UUAUC...AUC-3'@LB@ CUCUUUAU GCA GGACA UGACAU UCCGC@LB@ |||||:|| :|| ||||| |||||| |||||@LB@ GAGAAGUA UGU CCUGU ACUGUA AGGCG@LB@3'-AUU...AAAAU GU A AC UCGUU...CAA-5'@LB@ | |@LB@ 168 135@LB@@LB@@LB@Energy -16.32 kcal/mol@LB@Hybridization Energy -32.0013 kcal/mol@LB@Unfolding Energy - mRNA 8.50527 kcal/mol@LB@Unfolding Energy - sRNA 7.50175 kcal/mol@LB@@LB@Position - mRNA 85 -- 113@LB@Position - sRNA 136 -- 167@LB@Position Seed - mRNA 86 -- 92@LB@Position Seed - sRNA 160 -- 166@LB@ |
| 28 |
0.0008121 |
0.1059 |
bsu37450 |
ywhK |
-15.95 |
0.020994 |
89 -- 155 |
41 -- 105 |
Evidence 1a: Function experimentally demonstrated in the studied strain; PubMedId: 12073041; Product type f: factor |
bsu37440 |
_____________88_______________________________________156\n_____________|________________________________________|\n5'-UAU...CCUCA_______GUA_______ACA__U_____U____A______GUUAU...UUC-3'\n______________GCAAUUU___CAAGCAA___CG_UACCC_UC_C_UAUUCU\n______________|||||:|___|||:|||___||_|||||_||_|_|||||:\n______________CGUUAGA___GUUUGUU___GC_AUGGG_AG_G_AUAAGG\n3'-AUU...AGCAA_______ACC_______A__________U__A_A______GGUAA...CAA-5'\n_____________|________________________________________|\n_____________106______________________________________40\n |
127 -- 133 |
61 -- 67 |
-28.9674 kcal/mol |
4.84834 kcal/mol |
9.64723 kcal/mol |
88 156@LB@ | |@LB@5'-UAU...CCUCA GUA ACA U U A GUUAU...UUC-3'@LB@ GCAAUUU CAAGCAA CG UACCC UC C UAUUCU@LB@ |||||:| |||:||| || ||||| || | |||||:@LB@ CGUUAGA GUUUGUU GC AUGGG AG G AUAAGG@LB@3'-AUU...AGCAA ACC A U A A GGUAA...CAA-5'@LB@ | |@LB@ 106 40@LB@@LB@@LB@Energy -15.95 kcal/mol@LB@Hybridization Energy -28.9674 kcal/mol@LB@Unfolding Energy - mRNA 4.84834 kcal/mol@LB@Unfolding Energy - sRNA 9.64723 kcal/mol@LB@@LB@Position - mRNA 89 -- 155@LB@Position - sRNA 41 -- 105@LB@Position Seed - mRNA 127 -- 133@LB@Position Seed - sRNA 61 -- 67@LB@ |
| 55 |
0.001813 |
0.1212 |
bsu30290 |
amyC |
-12.90 |
0.089506 |
91 -- 153 |
102 -- 159 |
ABC transporter permease |
|
_____________90_________________________________________________________________154\n_____________|__________________________________________________________________|\n5'-CUG...UCAAA_________GCC___UA________AC_____G_______UU___GCAU__________GA_____AGCUU...CUU-3'\n______________CGGCAGGAU___GGC__UG_CCGCA__GCAAG___CAUAU__AUG____UUGUCAUUUU__UCAUC\n______________|::|||||:___|:|__||_|||||__|||||___|||||__|||____||||||:|||__|||||\n______________GUUGUCCUG___CUG__AC_GGCGU__CGUUC___GUAUA__UAC____AACAGUGAAA__AGUAG\n3'-AUU...AAGUA_________UAA___UA__A____________AAG_____U__________________G______AAGUU...CAA-5'\n_____________|__________________________________________________________________|\n_____________160________________________________________________________________101\n |
139 -- 145 |
109 -- 115 |
-48.9264 kcal/mol |
23.4207 kcal/mol |
14.7573 kcal/mol |
90 154@LB@ | |@LB@5'-CUG...UCAAA GCC UA AC G UU GCAU GA AGCUU...CUU-3'@LB@ CGGCAGGAU GGC UG CCGCA GCAAG CAUAU AUG UUGUCAUUUU UCAUC@LB@ |::|||||: |:| || ||||| ||||| ||||| ||| ||||||:||| |||||@LB@ GUUGUCCUG CUG AC GGCGU CGUUC GUAUA UAC AACAGUGAAA AGUAG@LB@3'-AUU...AAGUA UAA UA A AAG U G AAGUU...CAA-5'@LB@ | |@LB@ 160 101@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -48.9264 kcal/mol@LB@Unfolding Energy - mRNA 23.4207 kcal/mol@LB@Unfolding Energy - sRNA 14.7573 kcal/mol@LB@@LB@Position - mRNA 91 -- 153@LB@Position - sRNA 102 -- 159@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 109 -- 115@LB@ |
| 198 |
0.009004 |
0.1751 |
bsu38400 |
epr |
-15.65 |
0.024309 |
91 -- 136 |
32 -- 78 |
minor extracellular protease epr |
|
_____________90________________________________________________137\n_____________|_________________________________________________|\n5'-UGU...AAAUC__________C_______C_C_______GAAAAAUU_____________ACUAU...AAG-3'\n______________UGC_AUUUUG_UAAACAA_C_UGCCCAU________UUUUCC__UUCUU\n______________|||_||:|||_:||||||_|_|:|||||________||::||__|||||\n______________ACG_UAGAAC_GUUUGUU_G_AUGGGUA________AAGGGG__AAGAA\n3'-AUU...AAGCA___U______C_______A_C_______GAGAAU________UA_____ACUUU...CAA-5'\n_____________|_________________________________________________|\n_____________79________________________________________________31\n |
111 -- 117 |
51 -- 57 |
-30.7525 kcal/mol |
4.20051 kcal/mol |
13.8985 kcal/mol |
90 137@LB@ | |@LB@5'-UGU...AAAUC C C C GAAAAAUU ACUAU...AAG-3'@LB@ UGC AUUUUG UAAACAA C UGCCCAU UUUUCC UUCUU@LB@ ||| ||:||| :|||||| | |:||||| ||::|| |||||@LB@ ACG UAGAAC GUUUGUU G AUGGGUA AAGGGG AAGAA@LB@3'-AUU...AAGCA U C A C GAGAAU UA ACUUU...CAA-5'@LB@ | |@LB@ 79 31@LB@@LB@@LB@Energy -15.65 kcal/mol@LB@Hybridization Energy -30.7525 kcal/mol@LB@Unfolding Energy - mRNA 4.20051 kcal/mol@LB@Unfolding Energy - sRNA 13.8985 kcal/mol@LB@@LB@Position - mRNA 91 -- 136@LB@Position - sRNA 32 -- 78@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 51 -- 57@LB@ |
| 138 |
0.00639 |
0.1741 |
bsu24900 |
rpmG |
-18.16 |
0.007101 |
92 -- 175 |
92 -- 166 |
50S ribosomal protein L33 |
|
_____________91_________________________________________________________176\n_____________|__________________________________________________________|\n5'-CGC...GCGUA______AUAAU______AUU______AAAACAG________UUCCG____U_______GUAUU...AAA-3'\n______________UCUUCA_____UCAGCA___CAUUGA_______CC_UAGCA_____UCCA_UAUAGUG\n______________||||||_____|||:||___||||||_______||_:||||_____||||_||||:||\n______________AGAAGU_____AGUUGU___GUAACU_______GG_GUCGU_____AGGU_AUAUUAC\n3'-AUU...AAAUG_________________CCU______GUAACA___C_____UCA______________AACAG...CAA-5'\n_____________|__________________________________________________________|\n_____________167________________________________________________________91\n |
142 -- 148 |
118 -- 124 |
-35.8515 kcal/mol |
8.17901 kcal/mol |
10.6225 kcal/mol |
91 176@LB@ | |@LB@5'-CGC...GCGUA AUAAU AUU AAAACAG UUCCG U GUAUU...AAA-3'@LB@ UCUUCA UCAGCA CAUUGA CC UAGCA UCCA UAUAGUG@LB@ |||||| |||:|| |||||| || :|||| |||| ||||:||@LB@ AGAAGU AGUUGU GUAACU GG GUCGU AGGU AUAUUAC@LB@3'-AUU...AAAUG CCU GUAACA C UCA AACAG...CAA-5'@LB@ | |@LB@ 167 91@LB@@LB@@LB@Energy -18.16 kcal/mol@LB@Hybridization Energy -35.8515 kcal/mol@LB@Unfolding Energy - mRNA 8.17901 kcal/mol@LB@Unfolding Energy - sRNA 10.6225 kcal/mol@LB@@LB@Position - mRNA 92 -- 175@LB@Position - sRNA 92 -- 166@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 128 |
0.005903 |
0.1741 |
bsu24110 |
yqzF |
-12.53 |
0.106013 |
93 -- 138 |
39 -- 81 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________92_______________________________________________139\n_____________|________________________________________________|\n5'-UAA...UAUUC_________AAUUU___C___CCUA___UA___CGUUUUCU_______ACAAA...UCA-3'\n______________CGUUGCAAU_____GCA_AAC____UGU__CCA________UCUCUUG\n______________|||||||||_____|||_|||____:||__|||________||||||:\n______________GCAACGUUA_____CGU_UUG____GCA__GGU________AGAGAAU\n3'-AUU...CCGAA_________GAAC________UUA____UG__________________AAGGG...CAA-5'\n_____________|________________________________________________|\n_____________82_______________________________________________38\n |
134 -- 140 |
45 -- 51 |
-26.5343 kcal/mol |
8.17713 kcal/mol |
8.56905 kcal/mol |
92 139@LB@ | |@LB@5'-UAA...UAUUC AAUUU C CCUA UA CGUUUUCU ACAAA...UCA-3'@LB@ CGUUGCAAU GCA AAC UGU CCA UCUCUUG@LB@ ||||||||| ||| ||| :|| ||| ||||||:@LB@ GCAACGUUA CGU UUG GCA GGU AGAGAAU@LB@3'-AUU...CCGAA GAAC UUA UG AAGGG...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -26.5343 kcal/mol@LB@Unfolding Energy - mRNA 8.17713 kcal/mol@LB@Unfolding Energy - sRNA 8.56905 kcal/mol@LB@@LB@Position - mRNA 93 -- 138@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 129 |
0.005948 |
0.1741 |
bsu13580 |
ykrV |
-12.37 |
0.114104 |
93 -- 132 |
31 -- 61 |
transaminase MtnE |
|
_____________92________________________________________133\n_____________|_________________________________________|\n5'-CAG...AAGAU________AUUU________UAACA_____AAUU_______ACUUU...ACG-3'\n______________AUCGU_CC____CAUCUUUU_____CCCCA____UUUCUUU\n______________|||||_||____|||||:||_____|||||____|||||||\n______________UAGCA_GG____GUAGAGAA_____GGGGU____AAAGAAA\n3'-AUU...UUUGU_____U______________UAA__________________CUUUA...CAA-5'\n_____________|_________________________________________|\n_____________62________________________________________30\n |
126 -- 132 |
31 -- 37 |
-24.5638 kcal/mol |
3.82436 kcal/mol |
10.4555 kcal/mol |
92 133@LB@ | |@LB@5'-CAG...AAGAU AUUU UAACA AAUU ACUUU...ACG-3'@LB@ AUCGU CC CAUCUUUU CCCCA UUUCUUU@LB@ ||||| || |||||:|| ||||| |||||||@LB@ UAGCA GG GUAGAGAA GGGGU AAAGAAA@LB@3'-AUU...UUUGU U UAA CUUUA...CAA-5'@LB@ | |@LB@ 62 30@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -24.5638 kcal/mol@LB@Unfolding Energy - mRNA 3.82436 kcal/mol@LB@Unfolding Energy - sRNA 10.4555 kcal/mol@LB@@LB@Position - mRNA 93 -- 132@LB@Position - sRNA 31 -- 61@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 134 |
0.00626 |
0.1741 |
bsu03360 |
yciC |
-8.59 |
0.518951 |
99 -- 108 |
130 -- 139 |
metal chaperone YciC |
|
_____________98_______109\n_____________|________|\n5'-AGG...GGGCG________AAAUG...UAG-3'\n______________GCAGCGAG\n______________|||||:||\n______________CGUCGUUC\n3'-AUU...ACAGG________AAGGU...CAA-5'\n_____________|________|\n_____________140______129\n |
102 -- 108 |
130 -- 136 |
-12.856 kcal/mol |
2.76877 kcal/mol |
2.9981 kcal/mol |
98 109@LB@ | |@LB@5'-AGG...GGGCG AAAUG...UAG-3'@LB@ GCAGCGAG@LB@ |||||:||@LB@ CGUCGUUC@LB@3'-AUU...ACAGG AAGGU...CAA-5'@LB@ | |@LB@ 140 129@LB@@LB@@LB@Energy -8.59 kcal/mol@LB@Hybridization Energy -12.856 kcal/mol@LB@Unfolding Energy - mRNA 2.76877 kcal/mol@LB@Unfolding Energy - sRNA 2.9981 kcal/mol@LB@@LB@Position - mRNA 99 -- 108@LB@Position - sRNA 130 -- 139@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 189 |
0.008686 |
0.1751 |
bsu03110 |
ycgH |
-10.16 |
0.294825 |
102 -- 123 |
120 -- 141 |
transporter |
|
_____________101____________________124\n_____________|______________________|\n5'-UUU...UGAGC______UUCCUUC_________AAAGA...UCG-3'\n______________GUCUGC_______UCCAUGUAA\n______________|||:||_______|||||:|||\n______________CAGGCG_______AGGUAUAUU\n3'-AUU...UGUAA______UCGUUCA_________ACAAC...CAA-5'\n_____________|______________________|\n_____________142____________________119\n |
117 -- 123 |
120 -- 126 |
-19.5867 kcal/mol |
5.23504 kcal/mol |
6.76593 kcal/mol |
101 124@LB@ | |@LB@5'-UUU...UGAGC UUCCUUC AAAGA...UCG-3'@LB@ GUCUGC UCCAUGUAA@LB@ |||:|| |||||:|||@LB@ CAGGCG AGGUAUAUU@LB@3'-AUU...UGUAA UCGUUCA ACAAC...CAA-5'@LB@ | |@LB@ 142 119@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -19.5867 kcal/mol@LB@Unfolding Energy - mRNA 5.23504 kcal/mol@LB@Unfolding Energy - sRNA 6.76593 kcal/mol@LB@@LB@Position - mRNA 102 -- 123@LB@Position - sRNA 120 -- 141@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 124 |
0.005654 |
0.1741 |
bsu24680 |
comGF |
-17.71 |
0.008856 |
105 -- 249 |
40 -- 187 |
ComG operon protein 6 |
|
_____________104_____________________________________250\n_____________|_______________________________________|\n5'-UGU...UUUUG______A_______A_________GUU__A_A_______AAUGG...UAG-3'\n______________UC_UCG_CAGCAGG_ACA_UGACG___UC_C_CAGCAGG\n______________||_||:_||:||||_|||_||||:___||_|_|||||:|\n______________AG_AGU_GUUGUCC_UGU_ACUGU___AG_G_GUCGUUC\n3'-AUU...AAAUG__A___A___________A_____AAC____C_______AAGGU...CAA-5'\n_____________|_______________________________________|\n_____________188_____________________________________39\n |
276 -- 282 |
130 -- 136 |
-32.8645 kcal/mol |
8.22307 kcal/mol |
10.1234 kcal/mol |
104 250@LB@ | |@LB@5'-UGU...UUUUG A A GUU A A AAUGG...UAG-3'@LB@ UC UCG CAGCAGG ACA UGACG UC C CAGCAGG@LB@ || ||: ||:|||| ||| ||||: || | |||||:|@LB@ AG AGU GUUGUCC UGU ACUGU AG G GUCGUUC@LB@3'-AUU...AAAUG A A A AAC C AAGGU...CAA-5'@LB@ | |@LB@ 188 39@LB@@LB@@LB@Energy -17.71 kcal/mol@LB@Hybridization Energy -32.8645 kcal/mol@LB@Unfolding Energy - mRNA 8.22307 kcal/mol@LB@Unfolding Energy - sRNA 10.1234 kcal/mol@LB@@LB@Position - mRNA 105 -- 249@LB@Position - sRNA 40 -- 187@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 130 -- 136@LB@ |
