| 110 |
0.004746 |
0.1671 |
bsu09940 |
yhaL |
-13.90 |
0.056226 |
141 -- 159 |
37 -- 56 |
sporulation protein YhaL |
|
_____________140______________________________________160\n_____________|________________________________________|\n5'-GAA...UUUUU____________GUA___U____U___A_GCA________AGCGC...ACC-3'\n______________UCCGUGGUGGGU___UCU_UGUA_UGU_G___UCAUUUUC\n______________||||::|:::||___||:_|:||_|||_|___|||:||||\n______________AGGCGUCGUUCA___AGG_AUAU_ACA_C___AGUGAAAG\n3'-AUU...GUAAC__________________U____U___A____________AGUAG...CAA-5'\n_____________|________________________________________|\n_____________57_______________________________________36\n |
245 -- 251 |
107 -- 113 |
-27.9954 kcal/mol |
7.44184 kcal/mol |
9.97721 kcal/mol |
140 160@LB@ | |@LB@5'-GAA...UUUUU GUA U U A GCA AGCGC...ACC-3'@LB@ UCCGUGGUGGGU UCU UGUA UGU G UCAUUUUC@LB@ ||||::|:::|| ||: |:|| ||| | |||:||||@LB@ AGGCGUCGUUCA AGG AUAU ACA C AGUGAAAG@LB@3'-AUU...GUAAC U U A AGUAG...CAA-5'@LB@ | |@LB@ 57 36@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -27.9954 kcal/mol@LB@Unfolding Energy - mRNA 7.44184 kcal/mol@LB@Unfolding Energy - sRNA 9.97721 kcal/mol@LB@@LB@Position - mRNA 141 -- 159@LB@Position - sRNA 37 -- 56@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 139 |
0.006395 |
0.1741 |
bsu30170 |
ytcP |
-13.74 |
0.060669 |
6 -- 28 |
1 -- 23 |
ABC transporter permease |
|
_____________5_______________________29\n_____________|_______________________|\n______5'-UCUAA__UU_____CUU___________AUCGG...UUG-3'\n______________GA__AUGCU___UUUCUUUUGUU\n______________||__|:|||___|||||||||||\n______________CU__UGCGA___AAAGAAAACAA\n3'-AUU...UUAAA__UU_____AUU___________-5'\n_____________|______________________|\n_____________24_____________________1\n |
22 -- 28 |
1 -- 7 |
-18.7227 kcal/mol |
2.34195 kcal/mol |
4.12892 kcal/mol |
5 29@LB@ | |@LB@ 5'-UCUAA UU CUU AUCGG...UUG-3'@LB@ GA AUGCU UUUCUUUUGUU@LB@ || |:||| |||||||||||@LB@ CU UGCGA AAAGAAAACAA@LB@3'-AUU...UUAAA UU AUU -5'@LB@ | |@LB@ 24 1@LB@@LB@@LB@Energy -13.74 kcal/mol@LB@Hybridization Energy -18.7227 kcal/mol@LB@Unfolding Energy - mRNA 2.34195 kcal/mol@LB@Unfolding Energy - sRNA 4.12892 kcal/mol@LB@@LB@Position - mRNA 6 -- 28@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 1 -- 7@LB@ |
| 39 |
0.001272 |
0.1206 |
bsu38090 |
vpr |
-13.65 |
0.063330 |
246 -- 286 |
133 -- 174 |
minor extracellular protease vpr |
|
_____________245__________________________________________287\n_____________|____________________________________________|\n5'-AUU...CUUAU_______G_______C__________ACG______CAG______UCCGG...CAA-3'\n______________UUUUUGC___UUAUC_ACAGG_CAUU___GGCGUU____GCAGC\n______________|||||:|___|:|||_|||||_||||___|:|:||____|||||\n______________AAAAAUG___AGUAG_UGUCC_GUAA___CUGUAA____CGUCG\n3'-AUU...CGAAA_______AGA_____U_____U_____________CAGG_____UUCAA...CAA-5'\n_____________|____________________________________________|\n_____________175__________________________________________132\n |
247 -- 253 |
167 -- 173 |
-30.59 kcal/mol |
9.01512 kcal/mol |
10.7895 kcal/mol |
245 287@LB@ | |@LB@5'-AUU...CUUAU G C ACG CAG UCCGG...CAA-3'@LB@ UUUUUGC UUAUC ACAGG CAUU GGCGUU GCAGC@LB@ |||||:| |:||| ||||| |||| |:|:|| |||||@LB@ AAAAAUG AGUAG UGUCC GUAA CUGUAA CGUCG@LB@3'-AUU...CGAAA AGA U U CAGG UUCAA...CAA-5'@LB@ | |@LB@ 175 132@LB@@LB@@LB@Energy -13.65 kcal/mol@LB@Hybridization Energy -30.59 kcal/mol@LB@Unfolding Energy - mRNA 9.01512 kcal/mol@LB@Unfolding Energy - sRNA 10.7895 kcal/mol@LB@@LB@Position - mRNA 246 -- 286@LB@Position - sRNA 133 -- 174@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 167 -- 173@LB@ |
| 149 |
0.006788 |
0.1741 |
bsu28960 |
ytxC |
-13.61 |
0.064351 |
236 -- 300 |
132 -- 186 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________235________________________301\n_____________|__________________________|\n________5'-CGA__U________U______________CUUUC...GCA-3'\n______________GC__UUUUGGC_GGCAAUC_UGCCCG\n______________||__|:|||||_::|||||_|:|||:\n______________CG__AGAACCG_UUGUUAG_AUGGGU\n3'-AUU...AGCAA__UU_______U_______C______AGAGA...CAA-5'\n_____________|__________________________|\n_____________187________________________131\n |
7 -- 13 |
67 -- 73 |
-27.0931 kcal/mol |
7.22978 kcal/mol |
8.4393 kcal/mol |
235 301@LB@ | |@LB@ 5'-CGA U U CUUUC...GCA-3'@LB@ GC UUUUGGC GGCAAUC UGCCCG@LB@ || |:||||| ::||||| |:|||:@LB@ CG AGAACCG UUGUUAG AUGGGU@LB@3'-AUU...AGCAA UU U C AGAGA...CAA-5'@LB@ | |@LB@ 187 131@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -27.0931 kcal/mol@LB@Unfolding Energy - mRNA 7.22978 kcal/mol@LB@Unfolding Energy - sRNA 8.4393 kcal/mol@LB@@LB@Position - mRNA 236 -- 300@LB@Position - sRNA 132 -- 186@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 199 |
0.00902 |
0.1751 |
bsu30990 |
thiT |
-13.50 |
0.067771 |
270 -- 291 |
126 -- 147 |
thiamine transporter ThiT |
|
_____________269_____________________292\n_____________|_______________________|\n5'-AUG...UUUUA______________G__U_____UUAGC...UGC-3'\n______________GACAUUGUCU_CAG_AA_GUUUC\n______________|||||||||:_|||_||_|||:|\n______________CUGUAACAGG_GUC_UU_CAAGG\n3'-AUU...UGUAA__________C___G________UAUAU...CAA-5'\n_____________|_______________________|\n_____________148_____________________125\n |
273 -- 279 |
138 -- 144 |
-23.3567 kcal/mol |
5.16312 kcal/mol |
6.80985 kcal/mol |
269 292@LB@ | |@LB@5'-AUG...UUUUA G U UUAGC...UGC-3'@LB@ GACAUUGUCU CAG AA GUUUC@LB@ |||||||||: ||| || |||:|@LB@ CUGUAACAGG GUC UU CAAGG@LB@3'-AUU...UGUAA C G UAUAU...CAA-5'@LB@ | |@LB@ 148 125@LB@@LB@@LB@Energy -13.50 kcal/mol@LB@Hybridization Energy -23.3567 kcal/mol@LB@Unfolding Energy - mRNA 5.16312 kcal/mol@LB@Unfolding Energy - sRNA 6.80985 kcal/mol@LB@@LB@Position - mRNA 270 -- 291@LB@Position - sRNA 126 -- 147@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 138 -- 144@LB@ |
| 183 |
0.008402 |
0.1751 |
bsu03010 |
amhX |
-13.44 |
0.069669 |
23 -- 92 |
7 -- 86 |
amidohydrolase AmhX |
|
_____________22_______________________________________________________________________________________93\n_____________|________________________________________________________________________________________|\n5'-AAA...UCUUA____G____U______GC____________________AU___GAUCCA__________________________A______UA____GAAUA...CUU-3'\n______________GGCU_UGUU_CGG_CU__UGGCA________UACUCAU__UUC______UCCCC___UCU_____GUUUGAAAAU__UUAAU__UUCU\n______________||||_:|||_|::_||__|||||________|||:|||__:||______|||||___|||_____:||||||||:__|||||__||||\n______________CCGA_GCAA_GUU_GA__ACCGU________AUGGGUA__GAG______AGGGG___AGA_____UAAACUUUUG__AAUUA__AAGA\n3'-AUU...ACUUA____A____C___A_________UUGUUAGC____________AAUA_______UAA___AACUU__________CG___________AAACAA-5'\n_____________|________________________________________________________________________________________|\n_____________87_______________________________________________________________________________________6\n |
45 -- 51 |
51 -- 57 |
-41.3184 kcal/mol |
11.5757 kcal/mol |
17.8015 kcal/mol |
22 93@LB@ | |@LB@5'-AAA...UCUUA G U GC AU GAUCCA A UA GAAUA...CUU-3'@LB@ GGCU UGUU CGG CU UGGCA UACUCAU UUC UCCCC UCU GUUUGAAAAU UUAAU UUCU@LB@ |||| :||| |:: || ||||| |||:||| :|| ||||| ||| :||||||||: ||||| ||||@LB@ CCGA GCAA GUU GA ACCGU AUGGGUA GAG AGGGG AGA UAAACUUUUG AAUUA AAGA@LB@3'-AUU...ACUUA A C A UUGUUAGC AAUA UAA AACUU CG AAACAA-5'@LB@ | |@LB@ 87 6@LB@@LB@@LB@Energy -13.44 kcal/mol@LB@Hybridization Energy -41.3184 kcal/mol@LB@Unfolding Energy - mRNA 11.5757 kcal/mol@LB@Unfolding Energy - sRNA 17.8015 kcal/mol@LB@@LB@Position - mRNA 23 -- 92@LB@Position - sRNA 7 -- 86@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 51 -- 57@LB@ |
| 85 |
0.003124 |
0.1423 |
bsu15550 |
pyrF |
-13.42 |
0.070218 |
194 -- 221 |
50 -- 78 |
orotidine 5'-phosphate decarboxylase |
|
_____________193___________________________222\n_____________|_____________________________|\n5'-CCC...GGAGC__G____A__AA________C________AUCGC...AGG-3'\n______________UG_AAUC_UG__AAACAA_C_UGCCCAUC\n______________||_||||_||__||||||_|_|:||||||\n______________AC_UUAG_AC__UUUGUU_G_AUGGGUAG\n3'-AUU...AAGCA__G____A__CG______A_C________AGAAU...CAA-5'\n_____________|_____________________________|\n_____________79____________________________49\n |
215 -- 221 |
50 -- 56 |
-24.8332 kcal/mol |
3.07511 kcal/mol |
8.45904 kcal/mol |
193 222@LB@ | |@LB@5'-CCC...GGAGC G A AA C AUCGC...AGG-3'@LB@ UG AAUC UG AAACAA C UGCCCAUC@LB@ || |||| || |||||| | |:||||||@LB@ AC UUAG AC UUUGUU G AUGGGUAG@LB@3'-AUU...AAGCA G A CG A C AGAAU...CAA-5'@LB@ | |@LB@ 79 49@LB@@LB@@LB@Energy -13.42 kcal/mol@LB@Hybridization Energy -24.8332 kcal/mol@LB@Unfolding Energy - mRNA 3.07511 kcal/mol@LB@Unfolding Energy - sRNA 8.45904 kcal/mol@LB@@LB@Position - mRNA 194 -- 221@LB@Position - sRNA 50 -- 78@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 21 |
0.0005858 |
0.1011 |
bsu27120 |
sigV |
-13.38 |
0.071859 |
12 -- 26 |
38 -- 54 |
RNA polymerase sigma factor SigV |
|
______________12__________________________27\n______________|___________________________|\n___________5'-______GUUUG_________________AAAAC...AAG-3'\n______________AAUAAU_____UCAUCUCUUA__CCCCA\n______________||:|||_____:|||||||||__|||||\n______________UUGUUA_____GGUAGAGAAU__GGGGU\n3'-AUU...ACCGU______GCAUG__________AA_____AAAGA...CAA-5'\n_____________|____________________________|\n_____________55___________________________37\n |
15 -- 21 |
45 -- 51 |
-23.0819 kcal/mol |
1.75763 kcal/mol |
10.671 kcal/mol |
12 27@LB@ | |@LB@ 5'- GUUUG AAAAC...AAG-3'@LB@ AAUAAU UCAUCUCUUA CCCCA@LB@ ||:||| :||||||||| |||||@LB@ UUGUUA GGUAGAGAAU GGGGU@LB@3'-AUU...ACCGU GCAUG AA AAAGA...CAA-5'@LB@ | |@LB@ 55 37@LB@@LB@@LB@Energy -13.38 kcal/mol@LB@Hybridization Energy -23.0819 kcal/mol@LB@Unfolding Energy - mRNA 1.75763 kcal/mol@LB@Unfolding Energy - sRNA 10.671 kcal/mol@LB@@LB@Position - mRNA 12 -- 26@LB@Position - sRNA 38 -- 54@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 75 |
0.002405 |
0.1242 |
bsu01600 |
ybbA |
-13.26 |
0.075734 |
128 -- 177 |
34 -- 77 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 12354229 2974033; Product type pe: enzyme' |
|
_____________127______________________________________________178\n_____________|________________________________________________|\n5'-UCA...CGCAG___UC_________________________GGA_______________AUAUC...UUU-3'\n______________GCA__AUUUUGGCG_________AUCC_UC____GUUCCUUAUUUCUU\n______________|||__||:|||||:_________|:||_||____:||||::|||||||\n______________CGU__UAGAACCGU_________UGGG_AG____UAAGGGGUAAAGAA\n3'-AUU...AGCAA______________UUGUUAGCA____U__AGAA______________ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________78_______________________________________________33\n |
158 -- 164 |
32 -- 38 |
-31.1473 kcal/mol |
6.80918 kcal/mol |
13.8943 kcal/mol |
127 178@LB@ | |@LB@5'-UCA...CGCAG UC GGA AUAUC...UUU-3'@LB@ GCA AUUUUGGCG AUCC UC GUUCCUUAUUUCUU@LB@ ||| ||:|||||: |:|| || :||||::|||||||@LB@ CGU UAGAACCGU UGGG AG UAAGGGGUAAAGAA@LB@3'-AUU...AGCAA UUGUUAGCA U AGAA ACUUU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -13.26 kcal/mol@LB@Hybridization Energy -31.1473 kcal/mol@LB@Unfolding Energy - mRNA 6.80918 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 128 -- 177@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 48 |
0.001494 |
0.1206 |
bsu33660 |
rghRA |
-13.24 |
0.076349 |
28 -- 146 |
39 -- 166 |
HTH-type transcriptional repressor RghR |
bsu33670 |
_____________27_________________________________________________147\n_____________|__________________________________________________|\n5'-UAU...AUUAC_____A____AAC______U___U___U_______A____A_C_______UUUAG...UCU-3'\n______________GGCUU_UGUU____UUUUG_CAA_ACA_UU___CC_GUCU_C_UAUUCCC\n______________|||||_:|||____|:|||_|||_|||_|:___||_:|||_|_|||||||\n______________CCGAA_GCAA____AGAAC_GUU_UGU_AG___GG_UAGA_G_AUAAGGG\n3'-AUU...ACUUA__________CGUU_____C_______U__CAU__G______A_______GUAAA...CAA-5'\n_____________|__________________________________________________|\n_____________167________________________________________________38\n |
139 -- 145 |
40 -- 46 |
-27.8785 kcal/mol |
6.21563 kcal/mol |
10.9223 kcal/mol |
27 147@LB@ | |@LB@5'-UAU...AUUAC A AAC U U U A A C UUUAG...UCU-3'@LB@ GGCUU UGUU UUUUG CAA ACA UU CC GUCU C UAUUCCC@LB@ ||||| :||| |:||| ||| ||| |: || :||| | |||||||@LB@ CCGAA GCAA AGAAC GUU UGU AG GG UAGA G AUAAGGG@LB@3'-AUU...ACUUA CGUU C U CAU G A GUAAA...CAA-5'@LB@ | |@LB@ 167 38@LB@@LB@@LB@Energy -13.24 kcal/mol@LB@Hybridization Energy -27.8785 kcal/mol@LB@Unfolding Energy - mRNA 6.21563 kcal/mol@LB@Unfolding Energy - sRNA 10.9223 kcal/mol@LB@@LB@Position - mRNA 28 -- 146@LB@Position - sRNA 39 -- 166@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 40 -- 46@LB@ |
