| 138 |
0.00639 |
0.1741 |
bsu24900 |
rpmG |
-18.16 |
0.007101 |
92 -- 175 |
92 -- 166 |
50S ribosomal protein L33 |
|
_____________91_________________________________________________________176\n_____________|__________________________________________________________|\n5'-CGC...GCGUA______AUAAU______AUU______AAAACAG________UUCCG____U_______GUAUU...AAA-3'\n______________UCUUCA_____UCAGCA___CAUUGA_______CC_UAGCA_____UCCA_UAUAGUG\n______________||||||_____|||:||___||||||_______||_:||||_____||||_||||:||\n______________AGAAGU_____AGUUGU___GUAACU_______GG_GUCGU_____AGGU_AUAUUAC\n3'-AUU...AAAUG_________________CCU______GUAACA___C_____UCA______________AACAG...CAA-5'\n_____________|__________________________________________________________|\n_____________167________________________________________________________91\n |
142 -- 148 |
118 -- 124 |
-35.8515 kcal/mol |
8.17901 kcal/mol |
10.6225 kcal/mol |
91 176@LB@ | |@LB@5'-CGC...GCGUA AUAAU AUU AAAACAG UUCCG U GUAUU...AAA-3'@LB@ UCUUCA UCAGCA CAUUGA CC UAGCA UCCA UAUAGUG@LB@ |||||| |||:|| |||||| || :|||| |||| ||||:||@LB@ AGAAGU AGUUGU GUAACU GG GUCGU AGGU AUAUUAC@LB@3'-AUU...AAAUG CCU GUAACA C UCA AACAG...CAA-5'@LB@ | |@LB@ 167 91@LB@@LB@@LB@Energy -18.16 kcal/mol@LB@Hybridization Energy -35.8515 kcal/mol@LB@Unfolding Energy - mRNA 8.17901 kcal/mol@LB@Unfolding Energy - sRNA 10.6225 kcal/mol@LB@@LB@Position - mRNA 92 -- 175@LB@Position - sRNA 92 -- 166@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 15 |
0.0004798 |
0.1011 |
bsu04380 |
ydaT |
-18.00 |
0.007663 |
13 -- 34 |
33 -- 57 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________12________________________35\n_____________|_________________________|\n5'-AAC...CUUUU____G____________________CUAUC...AAG-3'\n______________UGCU_AUCUU___UUUCCCAUUUCU\n______________|:|:_||||:___||:|||||||||\n______________AUGG_UAGAG___AAGGGGUAAAGA\n3'-AUU...UUAGC____G_____AAU____________AACUU...CAA-5'\n_____________|_________________________|\n_____________58________________________32\n |
28 -- 34 |
33 -- 39 |
-24.9146 kcal/mol |
0.801206 kcal/mol |
9.08864 kcal/mol |
12 35@LB@ | |@LB@5'-AAC...CUUUU G CUAUC...AAG-3'@LB@ UGCU AUCUU UUUCCCAUUUCU@LB@ |:|: ||||: ||:|||||||||@LB@ AUGG UAGAG AAGGGGUAAAGA@LB@3'-AUU...UUAGC G AAU AACUU...CAA-5'@LB@ | |@LB@ 58 32@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -24.9146 kcal/mol@LB@Unfolding Energy - mRNA 0.801206 kcal/mol@LB@Unfolding Energy - sRNA 9.08864 kcal/mol@LB@@LB@Position - mRNA 13 -- 34@LB@Position - sRNA 33 -- 57@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 102 |
0.004212 |
0.1594 |
bsu10510 |
yhjH |
-17.94 |
0.007893 |
238 -- 265 |
152 -- 183 |
MarR family transcriptional regulator |
|
_____________237________________266\n_____________|__________________|\n5'-GCA...GCGGA____A_____________GAAAU...CUG-3'\n______________GCUC_UUCAUCAGCAGGA\n______________:|||_|||||||:|||||\n______________UGAG_AAGUAGUUGUCCU\n3'-AUU...AAAAA__________________GUAAC...CAA-5'\n_____________|__________________|\n_____________184________________151\n |
259 -- 265 |
152 -- 158 |
-25.8948 kcal/mol |
5.0064 kcal/mol |
6.06644 kcal/mol |
237 266@LB@ | |@LB@5'-GCA...GCGGA A GAAAU...CUG-3'@LB@ GCUC UUCAUCAGCAGGA@LB@ :||| |||||||:|||||@LB@ UGAG AAGUAGUUGUCCU@LB@3'-AUU...AAAAA GUAAC...CAA-5'@LB@ | |@LB@ 184 151@LB@@LB@@LB@Energy -17.94 kcal/mol@LB@Hybridization Energy -25.8948 kcal/mol@LB@Unfolding Energy - mRNA 5.0064 kcal/mol@LB@Unfolding Energy - sRNA 6.06644 kcal/mol@LB@@LB@Position - mRNA 238 -- 265@LB@Position - sRNA 152 -- 183@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 152 -- 158@LB@ |
| 71 |
0.002326 |
0.1242 |
bsu37230 |
ywjA |
-17.78 |
0.008577 |
195 -- 260 |
99 -- 147 |
ABC transporter ATP-binding protein |
|
_____________194___________________________________________________________261\n_____________|_____________________________________________________________|\n5'-UGA...GGGAA____GG__G_________U__UUUUCAUAUUAUAAA________AAC______________GACUU...UUC-3'\n______________GAUA__GU_CUGCGGCAA_UU_______________CCAUAUAA___GUUGUU_CUUUCUC\n______________||:|__||_|:||:||||_||_______________||||||||___|||||:_|||||||\n______________CUGU__CA_GGCGUCGUU_AA_______________GGUAUAUU___CAACAG_GAAAGAG\n3'-AUU...UGUAA____AA____________C_________________________A________U_______UAGAA...CAA-5'\n_____________|_____________________________________________________________|\n_____________148___________________________________________________________98\n |
248 -- 254 |
105 -- 111 |
-39.3136 kcal/mol |
10.514 kcal/mol |
13.0974 kcal/mol |
194 261@LB@ | |@LB@5'-UGA...GGGAA GG G U UUUUCAUAUUAUAAA AAC GACUU...UUC-3'@LB@ GAUA GU CUGCGGCAA UU CCAUAUAA GUUGUU CUUUCUC@LB@ ||:| || |:||:|||| || |||||||| |||||: |||||||@LB@ CUGU CA GGCGUCGUU AA GGUAUAUU CAACAG GAAAGAG@LB@3'-AUU...UGUAA AA C A U UAGAA...CAA-5'@LB@ | |@LB@ 148 98@LB@@LB@@LB@Energy -17.78 kcal/mol@LB@Hybridization Energy -39.3136 kcal/mol@LB@Unfolding Energy - mRNA 10.514 kcal/mol@LB@Unfolding Energy - sRNA 13.0974 kcal/mol@LB@@LB@Position - mRNA 195 -- 260@LB@Position - sRNA 99 -- 147@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 124 |
0.005654 |
0.1741 |
bsu24680 |
comGF |
-17.71 |
0.008856 |
105 -- 249 |
40 -- 187 |
ComG operon protein 6 |
|
_____________104_____________________________________250\n_____________|_______________________________________|\n5'-UGU...UUUUG______A_______A_________GUU__A_A_______AAUGG...UAG-3'\n______________UC_UCG_CAGCAGG_ACA_UGACG___UC_C_CAGCAGG\n______________||_||:_||:||||_|||_||||:___||_|_|||||:|\n______________AG_AGU_GUUGUCC_UGU_ACUGU___AG_G_GUCGUUC\n3'-AUU...AAAUG__A___A___________A_____AAC____C_______AAGGU...CAA-5'\n_____________|_______________________________________|\n_____________188_____________________________________39\n |
276 -- 282 |
130 -- 136 |
-32.8645 kcal/mol |
8.22307 kcal/mol |
10.1234 kcal/mol |
104 250@LB@ | |@LB@5'-UGU...UUUUG A A GUU A A AAUGG...UAG-3'@LB@ UC UCG CAGCAGG ACA UGACG UC C CAGCAGG@LB@ || ||: ||:|||| ||| ||||: || | |||||:|@LB@ AG AGU GUUGUCC UGU ACUGU AG G GUCGUUC@LB@3'-AUU...AAAUG A A A AAC C AAGGU...CAA-5'@LB@ | |@LB@ 188 39@LB@@LB@@LB@Energy -17.71 kcal/mol@LB@Hybridization Energy -32.8645 kcal/mol@LB@Unfolding Energy - mRNA 8.22307 kcal/mol@LB@Unfolding Energy - sRNA 10.1234 kcal/mol@LB@@LB@Position - mRNA 105 -- 249@LB@Position - sRNA 40 -- 187@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 30 |
0.0008394 |
0.1059 |
bsu25600 |
comER |
-17.70 |
0.008924 |
72 -- 169 |
82 -- 187 |
ComE operon protein 4 |
|
_____________71__________________________________________________170\n_____________|___________________________________________________|\n5'-AGC...AAUAA____UU____AU__U___UCAACCA_______GUGCA_________C____GUCAU...CAA-3'\n______________UUGC__UCUU__GC_GAU_______AUUCAUC_____UGUUCCCCG_UUUC\n______________||||__||||__||_:|:_______|::||||_____|:||||||:_||||\n______________AACG__AGAA__CG_UUG_______UGGGUAG_____AUAAGGGGU_AAAG\n3'-AUU...GAAGC____UU____C___U___UUAGCA________AGA________________AAACU...CAA-5'\n_____________|___________________________________________________|\n_____________188_________________________________________________81\n |
126 -- 132 |
38 -- 44 |
-31.3225 kcal/mol |
7.58891 kcal/mol |
13.8007 kcal/mol |
71 170@LB@ | |@LB@5'-AGC...AAUAA UU AU U UCAACCA GUGCA C GUCAU...CAA-3'@LB@ UUGC UCUU GC GAU AUUCAUC UGUUCCCCG UUUC@LB@ |||| |||| || :|: |::|||| |:||||||: ||||@LB@ AACG AGAA CG UUG UGGGUAG AUAAGGGGU AAAG@LB@3'-AUU...GAAGC UU C U UUAGCA AGA AAACU...CAA-5'@LB@ | |@LB@ 188 81@LB@@LB@@LB@Energy -17.70 kcal/mol@LB@Hybridization Energy -31.3225 kcal/mol@LB@Unfolding Energy - mRNA 7.58891 kcal/mol@LB@Unfolding Energy - sRNA 13.8007 kcal/mol@LB@@LB@Position - mRNA 72 -- 169@LB@Position - sRNA 82 -- 187@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 160 |
0.007403 |
0.1751 |
bsu11139 |
yizC |
-17.39 |
0.010400 |
11 -- 81 |
42 -- 107 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________10____________________________________________________________________________82\n_____________|_____________________________________________________________________________|\n5'-UUG...GACGA_______CG________UG______GG____________CAAAAUG_________AGCAA_____GAAGG_______AGCAG...UGA-3'\n______________CUCAUCU__UCAAAGUA__GAA_GG_______GCAGUCU_______GGUAAACGA_____AUCCA_____UUUAUUC\n______________|||||||__||||||||__|||_||_______|||:|||_______||:||||:|_____|:|||_____:||||||\n______________GAGUAGA__AGUUUCAU__CUU_CC_______CGUUAGA_______CCGUUUGUU_____UGGGU_____GAAUAAG\n3'-AUU...UGAAA_________________UA___A__GAAGCAA_______A_______________AGCA______AGA_________GGGUA...CAA-5'\n_____________|_____________________________________________________________________________|\n_____________108___________________________________________________________________________41\n |
75 -- 81 |
42 -- 48 |
-46.4368 kcal/mol |
15.8344 kcal/mol |
18.2654 kcal/mol |
10 82@LB@ | |@LB@5'-UUG...GACGA CG UG GG CAAAAUG AGCAA GAAGG AGCAG...UGA-3'@LB@ CUCAUCU UCAAAGUA GAA GG GCAGUCU GGUAAACGA AUCCA UUUAUUC@LB@ ||||||| |||||||| ||| || |||:||| ||:||||:| |:||| :||||||@LB@ GAGUAGA AGUUUCAU CUU CC CGUUAGA CCGUUUGUU UGGGU GAAUAAG@LB@3'-AUU...UGAAA UA A GAAGCAA A AGCA AGA GGGUA...CAA-5'@LB@ | |@LB@ 108 41@LB@@LB@@LB@Energy -17.39 kcal/mol@LB@Hybridization Energy -46.4368 kcal/mol@LB@Unfolding Energy - mRNA 15.8344 kcal/mol@LB@Unfolding Energy - sRNA 18.2654 kcal/mol@LB@@LB@Position - mRNA 11 -- 81@LB@Position - sRNA 42 -- 107@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 83 |
0.002931 |
0.1368 |
bsu18650 |
pelB |
-17.38 |
0.010445 |
17 -- 103 |
38 -- 142 |
pectin lyase |
|
_____________16________________________________________________________________________________________________________________104\n_____________|_________________________________________________________________________________________________________________|\n5'-AGG...UAAGC_________U_______G_____UCAA____________CUA_________________________A___U____UU__________________________AGUAA____ACAUU...CAA-3'\n______________UG_CUGCAG_CAA___C_GUGUA______UC_CUUUCUU___UUUCAA______________UUGUU_GUA_GUCU__UGGC__GCAAUC___UCUAUUUUUUA_____CCCA\n______________||_|:||||_|||___|_:|:||______||_||||||:___:|||||______________|:|||_|:|_:|||__||||__:|||||___:|:||:|:|||_____||||\n______________AC_GGCGUC_GUU___G_UAUAU______AG_GAAAGAG___GAAGUU______________AGCAA_CGU_UAGA__ACCG__UGUUAG___GGGUAGAGAAU_____GGGU\n3'-AUU...CUGUA__A__________CAA_G_____UACAAC__U_______UA_______UCAUUACUUACCGA____________________UU______CAU___________AAG______AAAGA...CAA-5'\n_____________|_________________________________________________________________________________________________________________|\n_____________143_______________________________________________________________________________________________________________37\n |
42 -- 48 |
105 -- 111 |
-52.303 kcal/mol |
11.0023 kcal/mol |
27.6117 kcal/mol |
16 104@LB@ | |@LB@5'-AGG...UAAGC U G UCAA CUA A U UU AGUAA ACAUU...CAA-3'@LB@ UG CUGCAG CAA C GUGUA UC CUUUCUU UUUCAA UUGUU GUA GUCU UGGC GCAAUC UCUAUUUUUUA CCCA@LB@ || |:|||| ||| | :|:|| || ||||||: :||||| |:||| |:| :||| |||| :||||| :|:||:|:||| ||||@LB@ AC GGCGUC GUU G UAUAU AG GAAAGAG GAAGUU AGCAA CGU UAGA ACCG UGUUAG GGGUAGAGAAU GGGU@LB@3'-AUU...CUGUA A CAA G UACAAC U UA UCAUUACUUACCGA UU CAU AAG AAAGA...CAA-5'@LB@ | |@LB@ 143 37@LB@@LB@@LB@Energy -17.38 kcal/mol@LB@Hybridization Energy -52.303 kcal/mol@LB@Unfolding Energy - mRNA 11.0023 kcal/mol@LB@Unfolding Energy - sRNA 27.6117 kcal/mol@LB@@LB@Position - mRNA 17 -- 103@LB@Position - sRNA 38 -- 142@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 200 |
0.009037 |
0.1751 |
bsu15760 |
prpC |
-17.37 |
0.010506 |
124 -- 148 |
133 -- 158 |
protein phosphatase PrpC |
|
_____________123_________________________149\n_____________|___________________________|\n5'-ACG...AAGAG___A_______________A_______CUGCG...UUG-3'\n______________AGC_AGGACA_UGACAUUG__CGCAGC\n______________|:|_||||||_||||||||__||||||\n______________UUG_UCCUGU_ACUGUAAC__GCGUCG\n3'-AUU...AGUAG__________A________AG______UUCAA...CAA-5'\n_____________|___________________________|\n_____________159_________________________132\n |
135 -- 141 |
141 -- 147 |
-32.4946 kcal/mol |
7.19227 kcal/mol |
8.48433 kcal/mol |
123 149@LB@ | |@LB@5'-ACG...AAGAG A A CUGCG...UUG-3'@LB@ AGC AGGACA UGACAUUG CGCAGC@LB@ |:| |||||| |||||||| ||||||@LB@ UUG UCCUGU ACUGUAAC GCGUCG@LB@3'-AUU...AGUAG A AG UUCAA...CAA-5'@LB@ | |@LB@ 159 132@LB@@LB@@LB@Energy -17.37 kcal/mol@LB@Hybridization Energy -32.4946 kcal/mol@LB@Unfolding Energy - mRNA 7.19227 kcal/mol@LB@Unfolding Energy - sRNA 8.48433 kcal/mol@LB@@LB@Position - mRNA 124 -- 148@LB@Position - sRNA 133 -- 158@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 141 -- 147@LB@ |
| 92 |
0.003598 |
0.1515 |
bsu22200 |
cotD |
-17.29 |
0.010920 |
45 -- 158 |
37 -- 130 |
spore coat protein D |
|
_____________44________________________________________________________________________________________________________________________159\n_____________|_________________________________________________________________________________________________________________________|\n5'-AUU...UACUU_____U___U___GUAUUU___A________GU_________CUU___A__CA_____AA_____CACUUUUAAAAUAG_____U___UCAG_____________________________AACUU...ACC-3'\n______________GUUCU_UGU_GUG______UUG_UUGCUUUU__GUUUUCAAA___UAA_GA___GCUU__UUGCA______________GUCUU_GCA____AACA__UGUACCC____CUUAUUUUUCAU\n______________||||:_|:|_:||______|||_|::||||:__:|:||||||___|||_||___||||__|||||______________:||||_|||____||||__:||||||____||||||:::|||\n______________CAAGG_AUA_UAC______AAC_AGUGAAAG__UAGAAGUUU___AUU_CU___CGAA__AACGU______________UAGAA_CGU____UUGU__GCAUGGG____GAAUAAGGGGUA\n3'-AUU...UCGUU_____U___U_____________________AG_________C_____A__UAC____GC________________________C___________UA_______UAGA____________AAGAA...CAA-5'\n_____________|_________________________________________________________________________________________________________________________|\n_____________131_______________________________________________________________________________________________________________________36\n |
147 -- 153 |
42 -- 48 |
-55.4941 kcal/mol |
20.5826 kcal/mol |
23.6024 kcal/mol |
44 159@LB@ | |@LB@5'-AUU...UACUU U U GUAUUU A GU CUU A CA AA CACUUUUAAAAUAG U UCAG AACUU...ACC-3'@LB@ GUUCU UGU GUG UUG UUGCUUUU GUUUUCAAA UAA GA GCUU UUGCA GUCUU GCA AACA UGUACCC CUUAUUUUUCAU@LB@ ||||: |:| :|| ||| |::||||: :|:|||||| ||| || |||| ||||| :|||| ||| |||| :|||||| ||||||:::|||@LB@ CAAGG AUA UAC AAC AGUGAAAG UAGAAGUUU AUU CU CGAA AACGU UAGAA CGU UUGU GCAUGGG GAAUAAGGGGUA@LB@3'-AUU...UCGUU U U AG C A UAC GC C UA UAGA AAGAA...CAA-5'@LB@ | |@LB@ 131 36@LB@@LB@@LB@Energy -17.29 kcal/mol@LB@Hybridization Energy -55.4941 kcal/mol@LB@Unfolding Energy - mRNA 20.5826 kcal/mol@LB@Unfolding Energy - sRNA 23.6024 kcal/mol@LB@@LB@Position - mRNA 45 -- 158@LB@Position - sRNA 37 -- 130@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 42 -- 48@LB@ |
