| 109 |
0.004595 |
0.1633 |
bsu02700 |
estA |
-7.85 |
0.646397 |
289 -- 295 |
181 -- 187 |
lipase EstA |
bsu08350 |
_____________288_______296\n_____________|_________|\n5'-AUC...CUUAA_________AAAAA...AAC-3'\n______________AAUUUCUUU\n______________|||||||||\n______________UUAAAGAAA\n3'-AUU...GCGAA_________ACAA-5'\n_____________|_________|\n_____________188_______180\n |
109 -- 115 |
5 -- 11 |
-6.84044 kcal/mol |
1.42932 kcal/mol |
0.176622 kcal/mol |
288 296@LB@ | |@LB@5'-AUC...CUUAA AAAAA...AAC-3'@LB@ AAUUUCUUU@LB@ |||||||||@LB@ UUAAAGAAA@LB@3'-AUU...GCGAA ACAA-5'@LB@ | |@LB@ 188 180@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -6.84044 kcal/mol@LB@Unfolding Energy - mRNA 1.42932 kcal/mol@LB@Unfolding Energy - sRNA 0.176622 kcal/mol@LB@@LB@Position - mRNA 289 -- 295@LB@Position - sRNA 181 -- 187@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 5 -- 11@LB@ |
| 29 |
0.000814 |
0.1059 |
bsu35860 |
pgdS |
-7.84 |
0.648005 |
23 -- 34 |
130 -- 140 |
gamma-dl-glutamyl hydrolase |
|
______________23________________________________35\n______________|_________________________________|\n___________5'-________GGAAAUGAAUAAAA___A________AAGAA...AUA-3'\n______________AUUGAUAU______________UCC_GCAGCAGG\n______________|||||:||______________|||_||||||:|\n______________UAACUGUA______________AGG_CGUCGUUC\n3'-AUU...UCCUG________AC________________________AAGGU...CAA-5'\n_____________|__________________________________|\n_____________141________________________________129\n |
28 -- 34 |
130 -- 136 |
-20.8842 kcal/mol |
7.79874 kcal/mol |
6.50607 kcal/mol |
23 35@LB@ | |@LB@ 5'- GGAAAUGAAUAAAA A AAGAA...AUA-3'@LB@ AUUGAUAU UCC GCAGCAGG@LB@ |||||:|| ||| ||||||:|@LB@ UAACUGUA AGG CGUCGUUC@LB@3'-AUU...UCCUG AC AAGGU...CAA-5'@LB@ | |@LB@ 141 129@LB@@LB@@LB@Energy -7.84 kcal/mol@LB@Hybridization Energy -20.8842 kcal/mol@LB@Unfolding Energy - mRNA 7.79874 kcal/mol@LB@Unfolding Energy - sRNA 6.50607 kcal/mol@LB@@LB@Position - mRNA 23 -- 34@LB@Position - sRNA 130 -- 140@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 164 |
0.007653 |
0.1751 |
bsu02110 |
ybyB |
-7.82 |
0.651200 |
271 -- 286 |
38 -- 56 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________270_____287\n_____________|_______|\n5'-UUG...AAAAG_______AAAUC...AAU-3'\n______________ACCCGUC\n______________||||:||\n______________UGGGUAG\n3'-AUU...UAGCA_______AGAAU...CAA-5'\n_____________|_______|\n_____________57______37\n |
271 -- 277 |
50 -- 56 |
-11.5721 kcal/mol |
0.816393 kcal/mol |
3.06627 kcal/mol |
270 287@LB@ | |@LB@5'-UUG...AAAAG AAAUC...AAU-3'@LB@ ACCCGUC@LB@ ||||:||@LB@ UGGGUAG@LB@3'-AUU...UAGCA AGAAU...CAA-5'@LB@ | |@LB@ 57 37@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -11.5721 kcal/mol@LB@Unfolding Energy - mRNA 0.816393 kcal/mol@LB@Unfolding Energy - sRNA 3.06627 kcal/mol@LB@@LB@Position - mRNA 271 -- 286@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 53 |
0.001762 |
0.1212 |
bsu10040 |
ecsA |
-7.56 |
0.695140 |
42 -- 51 |
33 -- 42 |
ABC transporter ATP-binding protein |
|
_____________41_________52\n_____________|__________|\n5'-UAU...AAACA__________CAGUU...GCU-3'\n______________CCUCAUUUUU\n______________||:|||||:|\n______________GGGGUAAAGA\n3'-AUU...AAUAA__________AACUU...CAA-5'\n_____________|__________|\n_____________43_________32\n |
45 -- 51 |
33 -- 39 |
-10.6739 kcal/mol |
0.405129 kcal/mol |
4.65815 kcal/mol |
41 52@LB@ | |@LB@5'-UAU...AAACA CAGUU...GCU-3'@LB@ CCUCAUUUUU@LB@ ||:|||||:|@LB@ GGGGUAAAGA@LB@3'-AUU...AAUAA AACUU...CAA-5'@LB@ | |@LB@ 43 32@LB@@LB@@LB@Energy -7.56 kcal/mol@LB@Hybridization Energy -10.6739 kcal/mol@LB@Unfolding Energy - mRNA 0.405129 kcal/mol@LB@Unfolding Energy - sRNA 4.65815 kcal/mol@LB@@LB@Position - mRNA 42 -- 51@LB@Position - sRNA 33 -- 42@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 13 |
0.0004662 |
0.1011 |
bsu39950 |
yxaJ |
-7.42 |
0.718209 |
218 -- 252 |
31 -- 81 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15743949 15849754 16850406; Product type pm: membrane component' |
|
_____________217_________________________________________253\n_____________|___________________________________________|\n5'-CAU...AAUAG___G______A__A_____________________GC______UUUUU...CGG-3'\n______________CGU_GCGAUC_UG_CAA__________CCAUUUCU__GUUUCU\n______________|||_||:|||_||_|||__________||||:|||__:||:|:\n______________GCA_CGUUAG_AC_GUU__________GGUAGAGA__UAAGGG\n3'-AUU...CCGAA___A______A__C___UGUUAGCAUG________A_______GUAAA...CAA-5'\n_____________|___________________________________________|\n_____________82__________________________________________30\n |
236 -- 242 |
47 -- 53 |
-25.2058 kcal/mol |
7.74885 kcal/mol |
10.909 kcal/mol |
217 253@LB@ | |@LB@5'-CAU...AAUAG G A A GC UUUUU...CGG-3'@LB@ CGU GCGAUC UG CAA CCAUUUCU GUUUCU@LB@ ||| ||:||| || ||| ||||:||| :||:|:@LB@ GCA CGUUAG AC GUU GGUAGAGA UAAGGG@LB@3'-AUU...CCGAA A A C UGUUAGCAUG A GUAAA...CAA-5'@LB@ | |@LB@ 82 30@LB@@LB@@LB@Energy -7.42 kcal/mol@LB@Hybridization Energy -25.2058 kcal/mol@LB@Unfolding Energy - mRNA 7.74885 kcal/mol@LB@Unfolding Energy - sRNA 10.909 kcal/mol@LB@@LB@Position - mRNA 218 -- 252@LB@Position - sRNA 31 -- 81@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 81 |
0.002798 |
0.1325 |
bsu03250 |
ycgR |
-7.37 |
0.727097 |
84 -- 130 |
48 -- 86 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pt: transporter' |
|
_____________83_______________________________________________131\n_____________|________________________________________________|\n5'-GCG...UACUC__________G____CUAACCAAAGUG________CUUUC___UC___CGUCA...GAA-3'\n______________GGCUUU_UUG_AAUC____________AAACGAUC_____CCC__CUC\n______________|||||:_|||_||||____________||||:|||_____|||__|||\n______________CCGAAG_AAC_UUAG____________UUUGUUAG_____GGG__GAG\n3'-AUU...ACUUA______C___G____AACCG_______________CAU_____UA___AAUAA...CAA-5'\n_____________|________________________________________________|\n_____________87_______________________________________________47\n |
111 -- 117 |
59 -- 65 |
-26.5811 kcal/mol |
9.91993 kcal/mol |
9.18845 kcal/mol |
83 131@LB@ | |@LB@5'-GCG...UACUC G CUAACCAAAGUG CUUUC UC CGUCA...GAA-3'@LB@ GGCUUU UUG AAUC AAACGAUC CCC CUC@LB@ |||||: ||| |||| ||||:||| ||| |||@LB@ CCGAAG AAC UUAG UUUGUUAG GGG GAG@LB@3'-AUU...ACUUA C G AACCG CAU UA AAUAA...CAA-5'@LB@ | |@LB@ 87 47@LB@@LB@@LB@Energy -7.37 kcal/mol@LB@Hybridization Energy -26.5811 kcal/mol@LB@Unfolding Energy - mRNA 9.91993 kcal/mol@LB@Unfolding Energy - sRNA 9.18845 kcal/mol@LB@@LB@Position - mRNA 84 -- 130@LB@Position - sRNA 48 -- 86@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 65 |
0.002089 |
0.123 |
bsu15330 |
sigG |
-7.32 |
0.735765 |
1 -- 8 |
133 -- 140 |
RNA polymerase sigma-G factor |
|
______________1_______9\n______________|_______|\n___________5'-________UUGAG...AUG-3'\n______________UUCGCGGC\n______________|:|||:||\n______________AGGCGUCG\n3'-AUU...GUAAC________UUCAA...CAA-5'\n_____________|________|\n_____________141______132\n |
2 -- 8 |
133 -- 139 |
-12.0754 kcal/mol |
1.63369 kcal/mol |
4.3229 kcal/mol |
1 9@LB@ | |@LB@ 5'- UUGAG...AUG-3'@LB@ UUCGCGGC@LB@ |:|||:||@LB@ AGGCGUCG@LB@3'-AUU...GUAAC UUCAA...CAA-5'@LB@ | |@LB@ 141 132@LB@@LB@@LB@Energy -7.32 kcal/mol@LB@Hybridization Energy -12.0754 kcal/mol@LB@Unfolding Energy - mRNA 1.63369 kcal/mol@LB@Unfolding Energy - sRNA 4.3229 kcal/mol@LB@@LB@Position - mRNA 1 -- 8@LB@Position - sRNA 133 -- 140@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 133 -- 139@LB@ |
| 111 |
0.004819 |
0.1671 |
bsu32120 |
yuzG |
-7.22 |
0.752372 |
165 -- 182 |
149 -- 166 |
Evidence 5: No homology to any previously reported sequences |
|
_____________164______________________________________183\n_____________|________________________________________|\n5'-UAG...UCUUU_____A_G_ACA__G__AAG__A________GAUG_____AAACG...UGG-3'\n______________AUGGC_U_G___UG_GA____G_GCGAAUAA____GCCCA\n______________|||||_|_|___||_:|____|_||:||:||____:||||\n______________UACCG_A_C___AC_UU____C_CGUUUGUU____UGGGU\n3'-AUU...UUACU_____A_G_A____G__AGAA__________AGCA_____AGAGA...CAA-5'\n_____________|________________________________________|\n_____________167______________________________________148\n |
192 -- 198 |
62 -- 68 |
-24.2214 kcal/mol |
8.24153 kcal/mol |
10.1588 kcal/mol |
164 183@LB@ | |@LB@5'-UAG...UCUUU A G ACA G AAG A GAUG AAACG...UGG-3'@LB@ AUGGC U G UG GA G GCGAAUAA GCCCA@LB@ ||||| | | || :| | ||:||:|| :||||@LB@ UACCG A C AC UU C CGUUUGUU UGGGU@LB@3'-AUU...UUACU A G A G AGAA AGCA AGAGA...CAA-5'@LB@ | |@LB@ 167 148@LB@@LB@@LB@Energy -7.22 kcal/mol@LB@Hybridization Energy -24.2214 kcal/mol@LB@Unfolding Energy - mRNA 8.24153 kcal/mol@LB@Unfolding Energy - sRNA 10.1588 kcal/mol@LB@@LB@Position - mRNA 165 -- 182@LB@Position - sRNA 149 -- 166@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 91 |
0.00349 |
0.1486 |
bsu28050 |
maf |
-6.37 |
0.874324 |
134 -- 184 |
39 -- 85 |
septum formation protein Maf |
|
_____________133_____185\n_____________|_______|\n5'-GAA...CCCUA_______AUUGU...AAG-3'\n______________CUCCAUU\n______________|:|||||\n______________GGGGUAA\n3'-AUU...AAUAA_______AGAAA...CAA-5'\n_____________|_______|\n_____________86______38\n |
275 -- 281 |
36 -- 42 |
-8.4297 kcal/mol |
0.414575 kcal/mol |
2.55634 kcal/mol |
133 185@LB@ | |@LB@5'-GAA...CCCUA AUUGU...AAG-3'@LB@ CUCCAUU@LB@ |:|||||@LB@ GGGGUAA@LB@3'-AUU...AAUAA AGAAA...CAA-5'@LB@ | |@LB@ 86 38@LB@@LB@@LB@Energy -6.37 kcal/mol@LB@Hybridization Energy -8.4297 kcal/mol@LB@Unfolding Energy - mRNA 0.414575 kcal/mol@LB@Unfolding Energy - sRNA 2.55634 kcal/mol@LB@@LB@Position - mRNA 134 -- 184@LB@Position - sRNA 39 -- 85@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 174 |
0.008073 |
0.1751 |
bsu36350 |
ywpD |
-6.29 |
0.883503 |
121 -- 192 |
80 -- 160 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 16740923; Product type prc : receptor' |
|
_____________120___________________________________193\n_____________|_____________________________________|\n5'-GAC...UCGUU______AAA_______________CGGU_____G___UGAAG...GAG-3'\n______________UCAACA___GCGU____AUUGUCC____GCAAG__CC\n______________||||||___:|:|____|||||||____|||||__||\n______________AGUUGU___UGUA____UAACAGG____CGUUC__GG\n3'-AUU...GAAGU______CC_____ACUG_______CGU______AA__UAUAU...CAA-5'\n_____________|_____________________________________|\n_____________161___________________________________79\n |
134 -- 140 |
138 -- 144 |
-24.1445 kcal/mol |
6.42035 kcal/mol |
10.3237 kcal/mol |
120 193@LB@ | |@LB@5'-GAC...UCGUU AAA CGGU G UGAAG...GAG-3'@LB@ UCAACA GCGU AUUGUCC GCAAG CC@LB@ |||||| :|:| ||||||| ||||| ||@LB@ AGUUGU UGUA UAACAGG CGUUC GG@LB@3'-AUU...GAAGU CC ACUG CGU AA UAUAU...CAA-5'@LB@ | |@LB@ 161 79@LB@@LB@@LB@Energy -6.29 kcal/mol@LB@Hybridization Energy -24.1445 kcal/mol@LB@Unfolding Energy - mRNA 6.42035 kcal/mol@LB@Unfolding Energy - sRNA 10.3237 kcal/mol@LB@@LB@Position - mRNA 121 -- 192@LB@Position - sRNA 80 -- 160@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 138 -- 144@LB@ |
