| 175 |
0.008121 |
0.1751 |
bsu19160 |
yocC |
-10.77 |
0.229075 |
5 -- 48 |
34 -- 79 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________4_________________________________________________49\n_____________|_________________________________________________|\n_______5'-UAAU___U_____AAUG___U________________CG_______CUU____ACAGG...UAU-3'\n______________UUG_UAAUC_____AA_CAAUCG___CCA_UUC__AUUCCUC___UUUC\n______________|||_:||||_____||_||||||___|||_:||__|||||:|___||||\n______________AAC_GUUAG_____UU_GUUAGC___GGU_GAG__UAAGGGG___AAAG\n3'-AUU...GAAGC_________AACCG__U______AUG___A___AA_______U______AAACU...CAA-5'\n_____________|_________________________________________________|\n_____________80________________________________________________33\n |
35 -- 41 |
39 -- 45 |
-25.1951 kcal/mol |
3.86331 kcal/mol |
13.8007 kcal/mol |
4 49@LB@ | |@LB@ 5'-UAAU U AAUG U CG CUU ACAGG...UAU-3'@LB@ UUG UAAUC AA CAAUCG CCA UUC AUUCCUC UUUC@LB@ ||| :|||| || |||||| ||| :|| |||||:| ||||@LB@ AAC GUUAG UU GUUAGC GGU GAG UAAGGGG AAAG@LB@3'-AUU...GAAGC AACCG U AUG A AA U AAACU...CAA-5'@LB@ | |@LB@ 80 33@LB@@LB@@LB@Energy -10.77 kcal/mol@LB@Hybridization Energy -25.1951 kcal/mol@LB@Unfolding Energy - mRNA 3.86331 kcal/mol@LB@Unfolding Energy - sRNA 13.8007 kcal/mol@LB@@LB@Position - mRNA 5 -- 48@LB@Position - sRNA 34 -- 79@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 181 |
0.008392 |
0.1751 |
bsu19600 |
yodH |
-10.70 |
0.236480 |
130 -- 154 |
156 -- 183 |
methyltransferase |
|
_____________129______________155\n_____________|________________|\n5'-AAC...CUUCU____________A___CUAUG...CCU-3'\n______________UUUGCUUUUCAU_AAC\n______________|||:||:|||||_|||\n______________AAAUGAGAAGUA_UUG\n3'-AUU...AAAAA____________G___UCCUG...CAA-5'\n_____________|________________|\n_____________184______________155\n |
144 -- 150 |
160 -- 166 |
-13.2566 kcal/mol |
3.14685 kcal/mol |
1.41519 kcal/mol |
129 155@LB@ | |@LB@5'-AAC...CUUCU A CUAUG...CCU-3'@LB@ UUUGCUUUUCAU AAC@LB@ |||:||:||||| |||@LB@ AAAUGAGAAGUA UUG@LB@3'-AUU...AAAAA G UCCUG...CAA-5'@LB@ | |@LB@ 184 155@LB@@LB@@LB@Energy -10.70 kcal/mol@LB@Hybridization Energy -13.2566 kcal/mol@LB@Unfolding Energy - mRNA 3.14685 kcal/mol@LB@Unfolding Energy - sRNA 1.41519 kcal/mol@LB@@LB@Position - mRNA 130 -- 154@LB@Position - sRNA 156 -- 183@LB@Position Seed - mRNA 144 -- 150@LB@Position Seed - sRNA 160 -- 166@LB@ |
| 116 |
0.005334 |
0.1741 |
bsu30510 |
ytpA |
-10.58 |
0.247824 |
123 -- 177 |
35 -- 87 |
phospholipase YtpA |
|
_____________122______________________________________________________178\n_____________|________________________________________________________|\n5'-AUU...AUAUC______G____C_UUAUAAG_______C__AAG___________CU__________AAAUU...GGC-3'\n______________UGGCUU_UGUU_C_______GGUAGAU_AU___AUUCAUC_CUU__UUCUCUAUUU\n______________||||||_:|||_|_______||:|:|:_||___|::||||_|||__|||:|:||||\n______________ACCGAA_GCAA_G_______CCGUUUG_UA___UGGGUAG_GAA__AAGGGGUAAA\n3'-AUU...UACUU___________C_UUAGAA________U__GCA_______A___U___________GAAAC...CAA-5'\n_____________|________________________________________________________|\n_____________88_______________________________________________________34\n |
171 -- 177 |
35 -- 41 |
-35.4104 kcal/mol |
13.324 kcal/mol |
14.1583 kcal/mol |
122 178@LB@ | |@LB@5'-AUU...AUAUC G C UUAUAAG C AAG CU AAAUU...GGC-3'@LB@ UGGCUU UGUU C GGUAGAU AU AUUCAUC CUU UUCUCUAUUU@LB@ |||||| :||| | ||:|:|: || |::|||| ||| |||:|:||||@LB@ ACCGAA GCAA G CCGUUUG UA UGGGUAG GAA AAGGGGUAAA@LB@3'-AUU...UACUU C UUAGAA U GCA A U GAAAC...CAA-5'@LB@ | |@LB@ 88 34@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -35.4104 kcal/mol@LB@Unfolding Energy - mRNA 13.324 kcal/mol@LB@Unfolding Energy - sRNA 14.1583 kcal/mol@LB@@LB@Position - mRNA 123 -- 177@LB@Position - sRNA 35 -- 87@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 123 |
0.005649 |
0.1741 |
bsu37530 |
ywhC |
-10.56 |
0.250674 |
18 -- 29 |
105 -- 116 |
zinc metalloprotease YwhC |
|
_____________17___________30\n_____________|____________|\n5'-GUU...GCUUC____________GACAA...UCG-3'\n______________UGUUACUUUCUC\n______________|||:||||||||\n______________ACAGUGAAAGAG\n3'-AUU...UUACA____________UAGAA...CAA-5'\n_____________|____________|\n_____________117__________104\n |
23 -- 29 |
105 -- 111 |
-16.5612 kcal/mol |
1.98074 kcal/mol |
5.11978 kcal/mol |
17 30@LB@ | |@LB@5'-GUU...GCUUC GACAA...UCG-3'@LB@ UGUUACUUUCUC@LB@ |||:||||||||@LB@ ACAGUGAAAGAG@LB@3'-AUU...UUACA UAGAA...CAA-5'@LB@ | |@LB@ 117 104@LB@@LB@@LB@Energy -10.56 kcal/mol@LB@Hybridization Energy -16.5612 kcal/mol@LB@Unfolding Energy - mRNA 1.98074 kcal/mol@LB@Unfolding Energy - sRNA 5.11978 kcal/mol@LB@@LB@Position - mRNA 18 -- 29@LB@Position - sRNA 105 -- 116@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 126 |
0.005872 |
0.1741 |
bsu36510 |
amtB |
-10.53 |
0.253946 |
51 -- 89 |
33 -- 57 |
ammonium transporter NrgA |
|
_____________50_____________________________________________________________________________________90\n_____________|______________________________________________________________________________________|\n______5'-CUCAU______A_____UUUUAUAGAUACAA______AUAUC____AAAA____AU____C_______UUCCAUCCCUCGAUAA_______CAAAA...GCA-3'\n______________AUGAAU_GUUUC______________AGUCUU_____GCAA____UGCC__UCUC___UUCCU________________CAUUUCU\n______________||||||_|:|||______________|:||||_____:|||____|:||__||||___||||:________________|||||||\n______________UACUUA_CGAAG______________UUAGAA_____UGUU____AUGG__AGAG___AAGGG________________GUAAAGA\n3'-AUU...UUCAU______C_____CAACG_______________CCGUU____AGC_____GU____AAU____________________________AACUU...CAA-5'\n_____________|______________________________________________________________________________________|\n_____________58_____________________________________________________________________________________32\n |
83 -- 89 |
33 -- 39 |
-30.7855 kcal/mol |
7.29289 kcal/mol |
15.7787 kcal/mol |
50 90@LB@ | |@LB@ 5'-CUCAU A UUUUAUAGAUACAA AUAUC AAAA AU C UUCCAUCCCUCGAUAA CAAAA...GCA-3'@LB@ AUGAAU GUUUC AGUCUU GCAA UGCC UCUC UUCCU CAUUUCU@LB@ |||||| |:||| |:|||| :||| |:|| |||| ||||: |||||||@LB@ UACUUA CGAAG UUAGAA UGUU AUGG AGAG AAGGG GUAAAGA@LB@3'-AUU...UUCAU C CAACG CCGUU AGC GU AAU AACUU...CAA-5'@LB@ | |@LB@ 58 32@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -30.7855 kcal/mol@LB@Unfolding Energy - mRNA 7.29289 kcal/mol@LB@Unfolding Energy - sRNA 15.7787 kcal/mol@LB@@LB@Position - mRNA 51 -- 89@LB@Position - sRNA 33 -- 57@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 45 |
0.001428 |
0.1206 |
bsu27290 |
yrzA |
-10.49 |
0.257926 |
1 -- 17 |
111 -- 136 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________0_________________18\n_____________|_________________|\n_______5'-CGGC_________________ACUCC...ACA-3'\n______________CCAUAU____UUGUCAC\n______________||||||____|||||||\n______________GGUAUA____AACAGUG\n3'-AUU...UUCAA______UUAC_______AAAGA...CAA-5'\n_____________|_________________|\n_____________137_______________110\n |
11 -- 17 |
111 -- 117 |
-14.7924 kcal/mol |
0.950105 kcal/mol |
3.76381 kcal/mol |
0 18@LB@ | |@LB@ 5'-CGGC ACUCC...ACA-3'@LB@ CCAUAU UUGUCAC@LB@ |||||| |||||||@LB@ GGUAUA AACAGUG@LB@3'-AUU...UUCAA UUAC AAAGA...CAA-5'@LB@ | |@LB@ 137 110@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -14.7924 kcal/mol@LB@Unfolding Energy - mRNA 0.950105 kcal/mol@LB@Unfolding Energy - sRNA 3.76381 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 111 -- 136@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 171 |
0.007883 |
0.1751 |
bsu38330 |
lrgB |
-10.47 |
0.260275 |
28 -- 48 |
159 -- 187 |
'Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 15659658 15849754 16850406; Product type r : regulator' |
|
_____________27____________49\n_____________|_____________|\n5'-GAG...CUGCU_____________CCGUC...UAU-3'\n______________UUUAUUUUUUAUC\n______________||||:|:||:|||\n______________AAAUGAGAAGUAG\n3'-AUU...AAAAA_____________UUGUC...CAA-5'\n_____________|_____________|\n_____________188___________158\n |
42 -- 48 |
159 -- 165 |
-9.66314 kcal/mol |
1.00478 kcal/mol |
0.734181 kcal/mol |
27 49@LB@ | |@LB@5'-GAG...CUGCU CCGUC...UAU-3'@LB@ UUUAUUUUUUAUC@LB@ ||||:|:||:|||@LB@ AAAUGAGAAGUAG@LB@3'-AUU...AAAAA UUGUC...CAA-5'@LB@ | |@LB@ 188 158@LB@@LB@@LB@Energy -10.47 kcal/mol@LB@Hybridization Energy -9.66314 kcal/mol@LB@Unfolding Energy - mRNA 1.00478 kcal/mol@LB@Unfolding Energy - sRNA 0.734181 kcal/mol@LB@@LB@Position - mRNA 28 -- 48@LB@Position - sRNA 159 -- 187@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 159 -- 165@LB@ |
| 31 |
0.0008564 |
0.1059 |
bsu10290 |
yhfN |
-10.46 |
0.260955 |
32 -- 88 |
131 -- 186 |
metalloprotease YhfN |
|
_____________31__________________89\n_____________|___________________|\n5'-AUG...GCAAG__________G________ACUUG...AAG-3'\n______________UGG_AUUG_C_GCAGCAGG\n______________||:_||||_|_||||||:|\n______________ACU_UAAC_G_CGUCGUUC\n3'-AUU...CUGUA___G____A_G________AAGGU...CAA-5'\n_____________|___________________|\n_____________187_________________130\n |
220 -- 226 |
130 -- 136 |
-18.5522 kcal/mol |
5.78917 kcal/mol |
6.22375 kcal/mol |
31 89@LB@ | |@LB@5'-AUG...GCAAG G ACUUG...AAG-3'@LB@ UGG AUUG C GCAGCAGG@LB@ ||: |||| | ||||||:|@LB@ ACU UAAC G CGUCGUUC@LB@3'-AUU...CUGUA G A G AAGGU...CAA-5'@LB@ | |@LB@ 187 130@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -18.5522 kcal/mol@LB@Unfolding Energy - mRNA 5.78917 kcal/mol@LB@Unfolding Energy - sRNA 6.22375 kcal/mol@LB@@LB@Position - mRNA 32 -- 88@LB@Position - sRNA 131 -- 186@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 72 |
0.002326 |
0.1242 |
bsu10730 |
yisI |
-10.35 |
0.272902 |
44 -- 108 |
96 -- 182 |
aspartyl-phosphate phosphatase YisI |
|
_____________43___________________________________109\n_____________|____________________________________|\n5'-UAG...AAAGG_________AAA________________________GUCAA...UGG-3'\n______________CCGCAGCAA_____________UUGUCGCUUUUUUA\n______________|||||||||_____________|||||:||||:|:|\n______________GGCGUCGUU_____________AACAGUGAAAGAGU\n3'-AUU...UAACA_________CAAGGUAUAUUAC______________AGAAG...CAA-5'\n_____________|____________________________________|\n_____________183__________________________________95\n |
90 -- 96 |
110 -- 116 |
-28.3207 kcal/mol |
8.15126 kcal/mol |
10.3722 kcal/mol |
43 109@LB@ | |@LB@5'-UAG...AAAGG AAA GUCAA...UGG-3'@LB@ CCGCAGCAA UUGUCGCUUUUUUA@LB@ ||||||||| |||||:||||:|:|@LB@ GGCGUCGUU AACAGUGAAAGAGU@LB@3'-AUU...UAACA CAAGGUAUAUUAC AGAAG...CAA-5'@LB@ | |@LB@ 183 95@LB@@LB@@LB@Energy -10.35 kcal/mol@LB@Hybridization Energy -28.3207 kcal/mol@LB@Unfolding Energy - mRNA 8.15126 kcal/mol@LB@Unfolding Energy - sRNA 10.3722 kcal/mol@LB@@LB@Position - mRNA 44 -- 108@LB@Position - sRNA 96 -- 182@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 110 -- 116@LB@ |
| 58 |
0.001848 |
0.1212 |
bsu21840 |
ypjP |
-10.31 |
0.277541 |
15 -- 73 |
14 -- 70 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________14___________________________________________________________74\n_____________|____________________________________________________________|\n5'-UAA...AACUG__________AG___CAA____GCGGAAAG____C____A_______A___U________UUACA...CCG-3'\n______________UGGCAAACAG__CGU___UCAU________CCUC_UUUC__UGAAAU_UUG_AAGCGUUU\n______________|||||||||:__|||___:|||________||:|_||||__||||||_|||_||:||:||\n______________ACCGUUUGUU__GCA___GGUA________GGGG_AAAG__ACUUUA_AAC_UUUGCGAA\n3'-AUU...UUAGA__________A____UG_____GAGAAUAA____U____AA__________U________UUAAA...CAA-5'\n_____________|____________________________________________________________|\n_____________71___________________________________________________________13\n |
67 -- 73 |
14 -- 20 |
-38.3558 kcal/mol |
13.3253 kcal/mol |
15.5628 kcal/mol |
14 74@LB@ | |@LB@5'-UAA...AACUG AG CAA GCGGAAAG C A A U UUACA...CCG-3'@LB@ UGGCAAACAG CGU UCAU CCUC UUUC UGAAAU UUG AAGCGUUU@LB@ |||||||||: ||| :||| ||:| |||| |||||| ||| ||:||:||@LB@ ACCGUUUGUU GCA GGUA GGGG AAAG ACUUUA AAC UUUGCGAA@LB@3'-AUU...UUAGA A UG GAGAAUAA U AA U UUAAA...CAA-5'@LB@ | |@LB@ 71 13@LB@@LB@@LB@Energy -10.31 kcal/mol@LB@Hybridization Energy -38.3558 kcal/mol@LB@Unfolding Energy - mRNA 13.3253 kcal/mol@LB@Unfolding Energy - sRNA 15.5628 kcal/mol@LB@@LB@Position - mRNA 15 -- 73@LB@Position - sRNA 14 -- 70@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 14 -- 20@LB@ |
