| 191 |
0.008727 |
0.1751 |
bsu31280 |
yugU |
-11.72 |
0.152832 |
2 -- 31 |
120 -- 152 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________1___________________________________32\n_____________|___________________________________|\n__________5'-A_______AA__GA______________________CAUCA...GCG-3'\n______________AUAUUGA__CA__G_CCGCA_CAAG___CAUAUGA\n______________|:|||||__||__|_|||||_||||___|||||:|\n______________UGUAACU__GU__C_GGCGU_GUUC___GUAUAUU\n3'-AUU...UGUCC___________AA_A_____C____AAG_______ACAAC...CAA-5'\n_____________|___________________________________|\n_____________153_________________________________119\n |
25 -- 31 |
120 -- 126 |
-21.956 kcal/mol |
3.20784 kcal/mol |
9.17624 kcal/mol |
1 32@LB@ | |@LB@ 5'-A AA GA CAUCA...GCG-3'@LB@ AUAUUGA CA G CCGCA CAAG CAUAUGA@LB@ |:||||| || | ||||| |||| |||||:|@LB@ UGUAACU GU C GGCGU GUUC GUAUAUU@LB@3'-AUU...UGUCC AA A C AAG ACAAC...CAA-5'@LB@ | |@LB@ 153 119@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -21.956 kcal/mol@LB@Unfolding Energy - mRNA 3.20784 kcal/mol@LB@Unfolding Energy - sRNA 9.17624 kcal/mol@LB@@LB@Position - mRNA 2 -- 31@LB@Position - sRNA 120 -- 152@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 168 |
0.007777 |
0.1751 |
bsu32270 |
yutH |
-11.70 |
0.153975 |
226 -- 259 |
121 -- 158 |
endospore coat-associated protein YutH |
|
_____________225____________________________________260\n_____________|______________________________________|\n5'-UGC...AGAAA____C______C____CAG_______U___________CCAGC...ACU-3'\n______________AAUA_GGCAUU_ACAU_____GCAGC___UUCCAUGUA\n______________||:|_|:||||_||||_____|||||___||||||:||\n______________UUGU_CUGUAA_UGUA_____CGUCG___AAGGUAUAU\n3'-AUU...AGUAG____C______C____ACAGG_____UUC_________UACAA...CAA-5'\n_____________|______________________________________|\n_____________159____________________________________120\n |
253 -- 259 |
121 -- 127 |
-28.4364 kcal/mol |
6.47482 kcal/mol |
10.499 kcal/mol |
225 260@LB@ | |@LB@5'-UGC...AGAAA C C CAG U CCAGC...ACU-3'@LB@ AAUA GGCAUU ACAU GCAGC UUCCAUGUA@LB@ ||:| |:|||| |||| ||||| ||||||:||@LB@ UUGU CUGUAA UGUA CGUCG AAGGUAUAU@LB@3'-AUU...AGUAG C C ACAGG UUC UACAA...CAA-5'@LB@ | |@LB@ 159 120@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -28.4364 kcal/mol@LB@Unfolding Energy - mRNA 6.47482 kcal/mol@LB@Unfolding Energy - sRNA 10.499 kcal/mol@LB@@LB@Position - mRNA 226 -- 259@LB@Position - sRNA 121 -- 158@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 14 |
0.0004671 |
0.1011 |
bsu04470 |
dctP |
-11.58 |
0.162472 |
124 -- 147 |
15 -- 44 |
C4-dicarboxylate transport protein |
bsu02340 bsu10220 |
_____________123____________________________148\n_____________|______________________________|\n5'-AAA...UAAAC_______GA__________A__________AUCAU...AAC-3'\n______________UUCCCCA___CU_____GU_UGAAAACGCU\n______________|||||||___||_____:|_||||||||||\n______________AAGGGGU___GA_____UA_ACUUUUGCGA\n3'-AUU...AGAAU_______AAA__AACUU__A__________AUUAA...CAA-5'\n_____________|______________________________|\n_____________45_____________________________14\n |
141 -- 147 |
15 -- 21 |
-23.6241 kcal/mol |
3.82582 kcal/mol |
8.2119 kcal/mol |
123 148@LB@ | |@LB@5'-AAA...UAAAC GA A AUCAU...AAC-3'@LB@ UUCCCCA CU GU UGAAAACGCU@LB@ ||||||| || :| ||||||||||@LB@ AAGGGGU GA UA ACUUUUGCGA@LB@3'-AUU...AGAAU AAA AACUU A AUUAA...CAA-5'@LB@ | |@LB@ 45 14@LB@@LB@@LB@Energy -11.58 kcal/mol@LB@Hybridization Energy -23.6241 kcal/mol@LB@Unfolding Energy - mRNA 3.82582 kcal/mol@LB@Unfolding Energy - sRNA 8.2119 kcal/mol@LB@@LB@Position - mRNA 124 -- 147@LB@Position - sRNA 15 -- 44@LB@Position Seed - mRNA 141 -- 147@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 148 |
0.006782 |
0.1741 |
bsu14050 |
ykuE |
-11.57 |
0.163142 |
1 -- 16 |
37 -- 54 |
metallophosphoesterase |
|
______________1__________________________17\n______________|__________________________|\n___________5'-_____G____________GC_GC____CCCGC...AUC-3'\n______________CCAUC_CU__UUUCCCAU__C__UUGA\n______________|||||_||__||:|||||__|__||||\n______________GGUAG_GA__AAGGGGUA__G__AACU\n3'-AUU...GCAUG_____A__AU________AA_A_____UUAAA...CAA-5'\n_____________|___________________________|\n_____________55__________________________36\n |
10 -- 16 |
37 -- 43 |
-20.8225 kcal/mol |
1.63511 kcal/mol |
9.4766 kcal/mol |
1 17@LB@ | |@LB@ 5'- G GC GC CCCGC...AUC-3'@LB@ CCAUC CU UUUCCCAU C UUGA@LB@ ||||| || ||:||||| | ||||@LB@ GGUAG GA AAGGGGUA G AACU@LB@3'-AUU...GCAUG A AU AA A UUAAA...CAA-5'@LB@ | |@LB@ 55 36@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -20.8225 kcal/mol@LB@Unfolding Energy - mRNA 1.63511 kcal/mol@LB@Unfolding Energy - sRNA 9.4766 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 140 |
0.006451 |
0.1741 |
bsu29090 |
polA |
-11.51 |
0.167440 |
58 -- 84 |
30 -- 56 |
DNA polymerase I |
|
_____________57________________85\n_____________|_________________|\n5'-AUG...CGGGA________G________GUCCU...AUA-3'\n______________ACCUAUUU__UGUUCCC\n______________|||:||:|__|:|||||\n______________UGGGUAGA__AUAAGGG\n3'-AUU...UAGCA________GA_______GUAAA...CAA-5'\n_____________|_________________|\n_____________57________________29\n |
67 -- 73 |
40 -- 46 |
-17.5976 kcal/mol |
2.41953 kcal/mol |
7.22212 kcal/mol |
57 85@LB@ | |@LB@5'-AUG...CGGGA G GUCCU...AUA-3'@LB@ ACCUAUUU UGUUCCC@LB@ |||:||:| |:|||||@LB@ UGGGUAGA AUAAGGG@LB@3'-AUU...UAGCA GA GUAAA...CAA-5'@LB@ | |@LB@ 57 29@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -17.5976 kcal/mol@LB@Unfolding Energy - mRNA 2.41953 kcal/mol@LB@Unfolding Energy - sRNA 7.22212 kcal/mol@LB@@LB@Position - mRNA 58 -- 84@LB@Position - sRNA 30 -- 56@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 163 |
0.007546 |
0.1751 |
bsu03210 |
ycgN |
-11.47 |
0.170088 |
123 -- 268 |
12 -- 147 |
1-pyrroline-5-carboxylate dehydrogenase |
|
_____________122______________________________________________________269\n_____________|________________________________________________________|\n5'-CAA...CCUUA__________A___AU__A_AA______AAGAUCAAAACAACG_____________AAAAG...UAG-3'\n______________CAAACA__CG_GCC__UC_C__AUUUCC_______________UGGAAGCGUUUAA\n______________||||||__||_:||__||_|__|||:||_______________||:||:||:||||\n______________GUUUGU__GC_UGG__AG_G__UAAGGG_______________ACUUUUGCGAAUU\n3'-AUU...GAACC______UA__A___GU__A_AA______GUAAAGAAACUUUAA_____________AAAGA...CAA-5'\n_____________|________________________________________________________|\n_____________148______________________________________________________11\n |
262 -- 268 |
12 -- 18 |
-30.9991 kcal/mol |
6.38057 kcal/mol |
15.527 kcal/mol |
122 269@LB@ | |@LB@5'-CAA...CCUUA A AU A AA AAGAUCAAAACAACG AAAAG...UAG-3'@LB@ CAAACA CG GCC UC C AUUUCC UGGAAGCGUUUAA@LB@ |||||| || :|| || | |||:|| ||:||:||:||||@LB@ GUUUGU GC UGG AG G UAAGGG ACUUUUGCGAAUU@LB@3'-AUU...GAACC UA A GU A AA GUAAAGAAACUUUAA AAAGA...CAA-5'@LB@ | |@LB@ 148 11@LB@@LB@@LB@Energy -11.47 kcal/mol@LB@Hybridization Energy -30.9991 kcal/mol@LB@Unfolding Energy - mRNA 6.38057 kcal/mol@LB@Unfolding Energy - sRNA 15.527 kcal/mol@LB@@LB@Position - mRNA 123 -- 268@LB@Position - sRNA 12 -- 147@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 12 -- 18@LB@ |
| 70 |
0.00232 |
0.1242 |
bsu24010 |
bmr |
-11.41 |
0.174600 |
110 -- 147 |
39 -- 86 |
multidrug resistance protein |
bsu26590 |
_____________109_______148\n_____________|_________|\n5'-CAA...ACAUG_________GCAAA...UGA-3'\n______________CUUGUUCCC\n______________|||:|||||\n______________GAAUAAGGG\n3'-AUU...GUAGA_________GUAAA...CAA-5'\n_____________|_________|\n_____________87________38\n |
82 -- 88 |
40 -- 46 |
-12.1575 kcal/mol |
2.82835 kcal/mol |
0.440898 kcal/mol |
109 148@LB@ | |@LB@5'-CAA...ACAUG GCAAA...UGA-3'@LB@ CUUGUUCCC@LB@ |||:|||||@LB@ GAAUAAGGG@LB@3'-AUU...GUAGA GUAAA...CAA-5'@LB@ | |@LB@ 87 38@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -12.1575 kcal/mol@LB@Unfolding Energy - mRNA 2.82835 kcal/mol@LB@Unfolding Energy - sRNA 0.440898 kcal/mol@LB@@LB@Position - mRNA 110 -- 147@LB@Position - sRNA 39 -- 86@LB@Position Seed - mRNA 82 -- 88@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 98 |
0.003906 |
0.1544 |
bsu29990 |
pbuO |
-11.33 |
0.180719 |
218 -- 233 |
123 -- 137 |
guanine/hypoxanthine permease PbuO |
|
_____________217______________234\n_____________|________________|\n5'-GGU...AAAGA_______ACA______AAGCA...CGG-3'\n______________GCAGCAA___UCCAUA\n______________|||||||___||||||\n______________CGUCGUU___AGGUAU\n3'-AUU...ACAGG_______CA_______AUUAC...CAA-5'\n_____________|________________|\n_____________138______________122\n |
218 -- 224 |
131 -- 137 |
-16.3971 kcal/mol |
2.46726 kcal/mol |
3.21202 kcal/mol |
217 234@LB@ | |@LB@5'-GGU...AAAGA ACA AAGCA...CGG-3'@LB@ GCAGCAA UCCAUA@LB@ ||||||| ||||||@LB@ CGUCGUU AGGUAU@LB@3'-AUU...ACAGG CA AUUAC...CAA-5'@LB@ | |@LB@ 138 122@LB@@LB@@LB@Energy -11.33 kcal/mol@LB@Hybridization Energy -16.3971 kcal/mol@LB@Unfolding Energy - mRNA 2.46726 kcal/mol@LB@Unfolding Energy - sRNA 3.21202 kcal/mol@LB@@LB@Position - mRNA 218 -- 233@LB@Position - sRNA 123 -- 137@LB@Position Seed - mRNA 218 -- 224@LB@Position Seed - sRNA 131 -- 137@LB@ |
| 77 |
0.002638 |
0.1315 |
bsu36790 |
ywmA |
-11.30 |
0.183092 |
1 -- 48 |
33 -- 81 |
Evidence 5: No homology to any previously reported sequences |
|
_____________0_______________49\n_____________|_______________|\n5'-CGA...GUUCA_______________AUAUA...GAC-3'\n______________UACCC_UUUCUUAUU\n______________|||||_|:|||||||\n______________AUGGG_AGAGAAUAA\n3'-AUU...UUAGC_____U_________GGGGU...CAA-5'\n_____________|_______________|\n_____________82______________32\n |
65 -- 71 |
43 -- 49 |
-14.8917 kcal/mol |
3.00255 kcal/mol |
4.80466 kcal/mol |
0 49@LB@ | |@LB@5'-CGA...GUUCA AUAUA...GAC-3'@LB@ UACCC UUUCUUAUU@LB@ ||||| |:|||||||@LB@ AUGGG AGAGAAUAA@LB@3'-AUU...UUAGC U GGGGU...CAA-5'@LB@ | |@LB@ 82 32@LB@@LB@@LB@Energy -11.30 kcal/mol@LB@Hybridization Energy -14.8917 kcal/mol@LB@Unfolding Energy - mRNA 3.00255 kcal/mol@LB@Unfolding Energy - sRNA 4.80466 kcal/mol@LB@@LB@Position - mRNA 1 -- 48@LB@Position - sRNA 33 -- 81@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 43 -- 49@LB@ |
| 44 |
0.001404 |
0.1206 |
bsu30660 |
ytkA |
-11.27 |
0.185588 |
215 -- 238 |
164 -- 186 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type lp: lipoprotein' |
|
_____________214_________239\n_____________|___________|\n5'-CAU...GCGCU___________GCGUU...UUU-3'\n______________AAACGUUUAAU\n______________|||||:|||||\n______________UUUGCGAAUUA\n3'-AUU...AAACU___________AAGAA...CAA-5'\n_____________|___________|\n_____________187_________163\n |
23 -- 29 |
11 -- 17 |
-8.86173 kcal/mol |
2.03186 kcal/mol |
2.83264 kcal/mol |
214 239@LB@ | |@LB@5'-CAU...GCGCU GCGUU...UUU-3'@LB@ AAACGUUUAAU@LB@ |||||:|||||@LB@ UUUGCGAAUUA@LB@3'-AUU...AAACU AAGAA...CAA-5'@LB@ | |@LB@ 187 163@LB@@LB@@LB@Energy -11.27 kcal/mol@LB@Hybridization Energy -8.86173 kcal/mol@LB@Unfolding Energy - mRNA 2.03186 kcal/mol@LB@Unfolding Energy - sRNA 2.83264 kcal/mol@LB@@LB@Position - mRNA 215 -- 238@LB@Position - sRNA 164 -- 186@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 11 -- 17@LB@ |
