| 82 |
0.002803 |
0.1325 |
bsu34100 |
rsbQ |
-17.18 |
0.011511 |
5 -- 86 |
34 -- 116 |
sigma factor SigB regulation protein RsbQ |
|
_____________4__________________________________________________________________________________________87\n_____________|__________________________________________________________________________________________|\n_______5'-CAAA____________C__ACCCC_____________G__UAG______CUCCUCCU___UCUUC_____C____________CUA__U_____GUCCU...UGU-3'\n______________UGUCAUUUUUUU_UC_______________GGU_UC___GCAAUC________GCA_____CAAUU__AUCCAUCUUUU___UC_CCCAU\n______________|||||:|||:|:_||_______________||:_||___||||||________|||_____||||:__|:||||||:||___||_|||||\n______________ACAGUGAAAGAG_AG_______________CCG_AG___CGUUAG________CGU_____GUUAG__UGGGUAGAGAA___AG_GGGUA\n3'-AUU...UUACA____________U__AAGUUUCAUUACUUA___A__CAA______AAC________UU________CA___________UA_________AAGAA...CAA-5'\n_____________|__________________________________________________________________________________________|\n_____________117________________________________________________________________________________________33\n |
65 -- 71 |
47 -- 53 |
-43.7075 kcal/mol |
8.64393 kcal/mol |
22.4324 kcal/mol |
4 87@LB@ | |@LB@ 5'-CAAA C ACCCC G UAG CUCCUCCU UCUUC C CUA U GUCCU...UGU-3'@LB@ UGUCAUUUUUUU UC GGU UC GCAAUC GCA CAAUU AUCCAUCUUUU UC CCCAU@LB@ |||||:|||:|: || ||: || |||||| ||| ||||: |:||||||:|| || |||||@LB@ ACAGUGAAAGAG AG CCG AG CGUUAG CGU GUUAG UGGGUAGAGAA AG GGGUA@LB@3'-AUU...UUACA U AAGUUUCAUUACUUA A CAA AAC UU CA UA AAGAA...CAA-5'@LB@ | |@LB@ 117 33@LB@@LB@@LB@Energy -17.18 kcal/mol@LB@Hybridization Energy -43.7075 kcal/mol@LB@Unfolding Energy - mRNA 8.64393 kcal/mol@LB@Unfolding Energy - sRNA 22.4324 kcal/mol@LB@@LB@Position - mRNA 5 -- 86@LB@Position - sRNA 34 -- 116@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 106 |
0.004415 |
0.1614 |
bsu10790 |
asnO |
-17.17 |
0.011578 |
3 -- 52 |
32 -- 73 |
asparagine synthetase 3 |
|
_____________2__________________________________________________53\n_____________|__________________________________________________|\n_________5'-CC___GUAUAU_________U________AUCCAUGCUG_____UU______CAUUC...CUG-3'\n______________UCU______AACAAUUGU_UCCGUUUC__________UCUCC__UUUCUU\n______________|||______||||||:||_:||:|:||__________||:||__||||||\n______________AGA______UUGUUAGCA_GGGUAGAG__________AGGGG__AAAGAA\n3'-AUU...ACGUU___ACCGU__________U________AAUA___________U_______ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________74_________________________________________________31\n |
23 -- 29 |
48 -- 54 |
-28.5385 kcal/mol |
1.12136 kcal/mol |
12.9474 kcal/mol |
2 53@LB@ | |@LB@ 5'-CC GUAUAU U AUCCAUGCUG UU CAUUC...CUG-3'@LB@ UCU AACAAUUGU UCCGUUUC UCUCC UUUCUU@LB@ ||| ||||||:|| :||:|:|| ||:|| ||||||@LB@ AGA UUGUUAGCA GGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACGUU ACCGU U AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 74 31@LB@@LB@@LB@Energy -17.17 kcal/mol@LB@Hybridization Energy -28.5385 kcal/mol@LB@Unfolding Energy - mRNA 1.12136 kcal/mol@LB@Unfolding Energy - sRNA 12.9474 kcal/mol@LB@@LB@Position - mRNA 3 -- 52@LB@Position - sRNA 32 -- 73@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 94 |
0.003675 |
0.1515 |
bsu40130 |
yydK |
-17.16 |
0.011624 |
17 -- 47 |
34 -- 60 |
HTH-type transcriptional regulator YydK |
|
_____________16___________________________________48\n_____________|____________________________________|\n__________5'-A_____UUU__U___C_________CUC_________CCCGA...UGG-3'\n______________UGUCU___GC_AGU_UCGUAUAAU____UCACUUUC\n______________||||:___||_|||_:|:||||||____||||||||\n______________ACAGG___CG_UCA_GGUAUAUUA____AGUGAAAG\n3'-AUU...CUGUA_____CGU__U___A_________CAAC________AGUAG...CAA-5'\n_____________|____________________________________|\n_____________61___________________________________33\n |
30 -- 36 |
107 -- 113 |
-26.8798 kcal/mol |
2.26958 kcal/mol |
10.0128 kcal/mol |
16 48@LB@ | |@LB@ 5'-A UUU U C CUC CCCGA...UGG-3'@LB@ UGUCU GC AGU UCGUAUAAU UCACUUUC@LB@ ||||: || ||| :|:|||||| ||||||||@LB@ ACAGG CG UCA GGUAUAUUA AGUGAAAG@LB@3'-AUU...CUGUA CGU U A CAAC AGUAG...CAA-5'@LB@ | |@LB@ 61 33@LB@@LB@@LB@Energy -17.16 kcal/mol@LB@Hybridization Energy -26.8798 kcal/mol@LB@Unfolding Energy - mRNA 2.26958 kcal/mol@LB@Unfolding Energy - sRNA 10.0128 kcal/mol@LB@@LB@Position - mRNA 17 -- 47@LB@Position - sRNA 34 -- 60@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 86 |
0.003162 |
0.1423 |
bsu32080 |
yuiB |
-17.14 |
0.011766 |
214 -- 271 |
6 -- 63 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________213____________________________________________________________272\n_____________|______________________________________________________________|\n5'-UAG...UCUGC________A_______AA_______G_U____GUC___UUU_______U_____________AUUAA...GGA-3'\n______________CAGUCGUA_UCAUCUC__UUGUUUU_U_UUUU___UUG___UUUGGAA_CG____GUUUCUU\n______________||:|||||_:||||||__||:||::_:_|||:___|||___||||:||_||____:||||||\n______________GUUAGCAU_GGUAGAG__AAUAAGG_G_AAAG___AAC___AAACUUU_GC____UAAAGAA\n3'-AUU...CGUUU________G________________G_U____A_____UUU_______U__GAAU_______AACAA-5'\n_____________|______________________________________________________________|\n_____________64_____________________________________________________________5\n |
265 -- 271 |
6 -- 12 |
-35.4392 kcal/mol |
4.76592 kcal/mol |
13.9327 kcal/mol |
213 272@LB@ | |@LB@5'-UAG...UCUGC A AA G U GUC UUU U AUUAA...GGA-3'@LB@ CAGUCGUA UCAUCUC UUGUUUU U UUUU UUG UUUGGAA CG GUUUCUU@LB@ ||:||||| :|||||| ||:||:: : |||: ||| ||||:|| || :||||||@LB@ GUUAGCAU GGUAGAG AAUAAGG G AAAG AAC AAACUUU GC UAAAGAA@LB@3'-AUU...CGUUU G G U A UUU U GAAU AACAA-5'@LB@ | |@LB@ 64 5@LB@@LB@@LB@Energy -17.14 kcal/mol@LB@Hybridization Energy -35.4392 kcal/mol@LB@Unfolding Energy - mRNA 4.76592 kcal/mol@LB@Unfolding Energy - sRNA 13.9327 kcal/mol@LB@@LB@Position - mRNA 214 -- 271@LB@Position - sRNA 6 -- 63@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 6 -- 12@LB@ |
| 38 |
0.001252 |
0.1206 |
bsu19610 |
yodI |
-17.13 |
0.011794 |
8 -- 27 |
49 -- 68 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________7____________________28\n_____________|____________________|\n____5'-AUUUAUC___________AU_______AUUAC...UAA-3'\n______________GCAAACGAUUG__CCCAUCU\n______________||||||:||:|__|||||||\n______________CGUUUGUUAGC__GGGUAGA\n3'-AUU...AGAAC___________AU_______GAAUA...CAA-5'\n_____________|____________________|\n_____________69___________________48\n |
21 -- 27 |
49 -- 55 |
-26.3851 kcal/mol |
1.36402 kcal/mol |
8.38306 kcal/mol |
7 28@LB@ | |@LB@ 5'-AUUUAUC AU AUUAC...UAA-3'@LB@ GCAAACGAUUG CCCAUCU@LB@ ||||||:||:| |||||||@LB@ CGUUUGUUAGC GGGUAGA@LB@3'-AUU...AGAAC AU GAAUA...CAA-5'@LB@ | |@LB@ 69 48@LB@@LB@@LB@Energy -17.13 kcal/mol@LB@Hybridization Energy -26.3851 kcal/mol@LB@Unfolding Energy - mRNA 1.36402 kcal/mol@LB@Unfolding Energy - sRNA 8.38306 kcal/mol@LB@@LB@Position - mRNA 8 -- 27@LB@Position - sRNA 49 -- 68@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 7 |
0.0001858 |
0.08823 |
bsu34210 |
yvfG |
-17.07 |
0.012126 |
85 -- 142 |
32 -- 86 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________84____________________________________________________________143\n_____________|_____________________________________________________________|\n5'-GAG...GUUGA______AAUAC___________C____GUU__A__________GCUCAC____________CAUUG...ACU-3'\n______________GGCUUU_____UUGUA__CUUG_UGAA___UC_UAUCCAUCUU______UCCCC_UUUCUU\n______________|||||:_____|||:|__||||_::||___||_||:||||||:______|||||_||||||\n______________CCGAAG_____AACGU__GAAC_GUUU___AG_AUGGGUAGAG______AGGGG_AAAGAA\n3'-AUU...ACUUA______C_________UA____C____GUU__C__________AAUA_______U______ACUUU...CAA-5'\n_____________|_____________________________________________________________|\n_____________87____________________________________________________________31\n |
119 -- 125 |
48 -- 54 |
-40.7387 kcal/mol |
11.2598 kcal/mol |
14.8836 kcal/mol |
84 143@LB@ | |@LB@5'-GAG...GUUGA AAUAC C GUU A GCUCAC CAUUG...ACU-3'@LB@ GGCUUU UUGUA CUUG UGAA UC UAUCCAUCUU UCCCC UUUCUU@LB@ |||||: |||:| |||| ::|| || ||:||||||: ||||| ||||||@LB@ CCGAAG AACGU GAAC GUUU AG AUGGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACUUA C UA C GUU C AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 87 31@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -40.7387 kcal/mol@LB@Unfolding Energy - mRNA 11.2598 kcal/mol@LB@Unfolding Energy - sRNA 14.8836 kcal/mol@LB@@LB@Position - mRNA 85 -- 142@LB@Position - sRNA 32 -- 86@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 27 |
0.0007779 |
0.1059 |
bsu31480 |
yuxJ |
-17.06 |
0.012234 |
48 -- 110 |
4 -- 55 |
MFS transporter |
|
_____________47______________________________________________________________111\n_____________|_______________________________________________________________|\n5'-UGC...AAUUC___U____GACAU___________CUAA____AA____UUUUCCCAGC_____C_________AAAUU...UUG-3'\n______________CCC_UCUC_____UUCUUCAUUUU____AAAU__UGAA__________CGCUU_GUUUCUUUU\n______________|||_||||_____|||::|||||:____||||__||||__________|||||_:||||||||\n______________GGG_AGAG_____AAGGGGUAAAG____UUUA__ACUU__________GCGAA_UAAAGAAAA\n3'-AUU...AGCAU___U____AAU_____________AAAC____A_____UU_____________U_________CAA-5'\n_____________|_______________________________________________________________|\n_____________56______________________________________________________________3\n |
104 -- 110 |
4 -- 10 |
-33.1771 kcal/mol |
7.88293 kcal/mol |
11.7347 kcal/mol |
47 111@LB@ | |@LB@5'-UGC...AAUUC U GACAU CUAA AA UUUUCCCAGC C AAAUU...UUG-3'@LB@ CCC UCUC UUCUUCAUUUU AAAU UGAA CGCUU GUUUCUUUU@LB@ ||| |||| |||::|||||: |||| |||| ||||| :||||||||@LB@ GGG AGAG AAGGGGUAAAG UUUA ACUU GCGAA UAAAGAAAA@LB@3'-AUU...AGCAU U AAU AAAC A UU U CAA-5'@LB@ | |@LB@ 56 3@LB@@LB@@LB@Energy -17.06 kcal/mol@LB@Hybridization Energy -33.1771 kcal/mol@LB@Unfolding Energy - mRNA 7.88293 kcal/mol@LB@Unfolding Energy - sRNA 11.7347 kcal/mol@LB@@LB@Position - mRNA 48 -- 110@LB@Position - sRNA 4 -- 55@LB@Position Seed - mRNA 104 -- 110@LB@Position Seed - sRNA 4 -- 10@LB@ |
| 158 |
0.007267 |
0.1751 |
bsu33000 |
htrB |
-17.04 |
0.012352 |
105 -- 237 |
50 -- 180 |
serine protease Do-like HtrB |
|
_____________104_____________________________238\n_____________|_______________________________|\n5'-UUU...GUGCU_______UCUCC______U______A_____ACUUG...CUG-3'\n______________UUGCUUU_____UCAUUA_UGGGAC_AUUGA\n______________||:||:|_____||||:|_::||||_|||||\n______________AAUGAGA_____AGUAGU_GUCCUG_UAACU\n3'-AUU...AAAAA__________________U____________GUAAC...CAA-5'\n_____________|_______________________________|\n_____________181_____________________________49\n |
110 -- 116 |
164 -- 170 |
-21.2082 kcal/mol |
7.59468 kcal/mol |
7.14638 kcal/mol |
104 238@LB@ | |@LB@5'-UUU...GUGCU UCUCC U A ACUUG...CUG-3'@LB@ UUGCUUU UCAUUA UGGGAC AUUGA@LB@ ||:||:| ||||:| ::|||| |||||@LB@ AAUGAGA AGUAGU GUCCUG UAACU@LB@3'-AUU...AAAAA U GUAAC...CAA-5'@LB@ | |@LB@ 181 49@LB@@LB@@LB@Energy -17.04 kcal/mol@LB@Hybridization Energy -21.2082 kcal/mol@LB@Unfolding Energy - mRNA 7.59468 kcal/mol@LB@Unfolding Energy - sRNA 7.14638 kcal/mol@LB@@LB@Position - mRNA 105 -- 237@LB@Position - sRNA 50 -- 180@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 164 -- 170@LB@ |
| 9 |
0.0002071 |
0.08823 |
bsu08425 |
mprF |
-16.98 |
0.012672 |
3 -- 50 |
34 -- 81 |
phosphatidylglycerol lysyltransferase |
|
_____________2_______________________________________________51\n_____________|_______________________________________________|\n_________5'-AC__________GU_______U__AU__________AUCAC________CAUUC...AUC-3'\n______________UGUUGU_AUC___GUAAAU_AU____UCCAUUUC_____UUCCUCAU\n______________:||||:_|||___|:|||:_||____:||||:||_____||||:|||\n______________GCAACG_UAG___CGUUUG_UA____GGGUAGAG_____AAGGGGUA\n3'-AUU...CCGAA______U___AAC______U__GCAU________AAU__________AAGAA...CAA-5'\n_____________|_______________________________________________|\n_____________82______________________________________________33\n |
39 -- 45 |
37 -- 43 |
-29.0114 kcal/mol |
1.80779 kcal/mol |
13.626 kcal/mol |
2 51@LB@ | |@LB@ 5'-AC GU U AU AUCAC CAUUC...AUC-3'@LB@ UGUUGU AUC GUAAAU AU UCCAUUUC UUCCUCAU@LB@ :||||: ||| |:|||: || :||||:|| ||||:|||@LB@ GCAACG UAG CGUUUG UA GGGUAGAG AAGGGGUA@LB@3'-AUU...CCGAA U AAC U GCAU AAU AAGAA...CAA-5'@LB@ | |@LB@ 82 33@LB@@LB@@LB@Energy -16.98 kcal/mol@LB@Hybridization Energy -29.0114 kcal/mol@LB@Unfolding Energy - mRNA 1.80779 kcal/mol@LB@Unfolding Energy - sRNA 13.626 kcal/mol@LB@@LB@Position - mRNA 3 -- 50@LB@Position - sRNA 34 -- 81@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 182 |
0.008397 |
0.1751 |
bsu38410 |
sacX |
-16.96 |
0.012854 |
69 -- 151 |
101 -- 169 |
negative regulator of SacY activity |
|
_____________68_____________________________________________________________________________________152\n_____________|______________________________________________________________________________________|\n5'-ACA...UCUCA_____CG_________C_______U________AUUUU____C___AUUUCUCACUUUU_____UU____A______AC_______UUGAU...UAA-3'\n______________UGCUC__UCGUCGACG_GGGCAUU____UUGUC_____CAGC_GGU_____________UUCAU____UG_UUGUCA__UUUUCAU\n______________|:|||__||:||:||:_||:||||____|||||_____||||_:||_____________|:|||____||_||||||__||:||||\n______________AUGAG__AGUAGUUGU_CCUGUAA____AACAG_____GUCG_UCA_____________AGGUA____AC_AACAGU__AAGAGUA\n3'-AUU...AAAAA_____A__________________CUGU_____GC_______U_____________________UAUU_________GA_______GAAGU...CAA-5'\n_____________|______________________________________________________________________________________|\n_____________170____________________________________________________________________________________100\n |
143 -- 149 |
103 -- 109 |
-47.5037 kcal/mol |
18.4019 kcal/mol |
14.5039 kcal/mol |
68 152@LB@ | |@LB@5'-ACA...UCUCA CG C U AUUUU C AUUUCUCACUUUU UU A AC UUGAU...UAA-3'@LB@ UGCUC UCGUCGACG GGGCAUU UUGUC CAGC GGU UUCAU UG UUGUCA UUUUCAU@LB@ |:||| ||:||:||: ||:|||| ||||| |||| :|| |:||| || |||||| ||:||||@LB@ AUGAG AGUAGUUGU CCUGUAA AACAG GUCG UCA AGGUA AC AACAGU AAGAGUA@LB@3'-AUU...AAAAA A CUGU GC U UAUU GA GAAGU...CAA-5'@LB@ | |@LB@ 170 100@LB@@LB@@LB@Energy -16.96 kcal/mol@LB@Hybridization Energy -47.5037 kcal/mol@LB@Unfolding Energy - mRNA 18.4019 kcal/mol@LB@Unfolding Energy - sRNA 14.5039 kcal/mol@LB@@LB@Position - mRNA 69 -- 151@LB@Position - sRNA 101 -- 169@LB@Position Seed - mRNA 143 -- 149@LB@Position Seed - sRNA 103 -- 109@LB@ |
