| 66 |
0.002095 |
0.123 |
bsu28660 |
sspI |
-12.58 |
0.103691 |
257 -- 278 |
56 -- 77 |
small acid-soluble spore protein I |
|
_____________256_____________________279\n_____________|_______________________|\n5'-UUG...CAGGA_________A___U_________GAUGC...AAG-3'\n______________GCAG_CUUG_GCA_ACAAUCGUA\n______________|||:_||||_|||_|||||||||\n______________CGUU_GAAC_CGU_UGUUAGCAU\n3'-AUU...AGCAA____A________U_________GGGUA...CAA-5'\n_____________|_______________________|\n_____________78______________________55\n |
271 -- 277 |
57 -- 63 |
-25.139 kcal/mol |
6.36558 kcal/mol |
6.79705 kcal/mol |
256 279@LB@ | |@LB@5'-UUG...CAGGA A U GAUGC...AAG-3'@LB@ GCAG CUUG GCA ACAAUCGUA@LB@ |||: |||| ||| |||||||||@LB@ CGUU GAAC CGU UGUUAGCAU@LB@3'-AUU...AGCAA A U GGGUA...CAA-5'@LB@ | |@LB@ 78 55@LB@@LB@@LB@Energy -12.58 kcal/mol@LB@Hybridization Energy -25.139 kcal/mol@LB@Unfolding Energy - mRNA 6.36558 kcal/mol@LB@Unfolding Energy - sRNA 6.79705 kcal/mol@LB@@LB@Position - mRNA 257 -- 278@LB@Position - sRNA 56 -- 77@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 57 -- 63@LB@ |
| 157 |
0.007221 |
0.1751 |
bsu40660 |
yybF |
-12.55 |
0.105157 |
243 -- 270 |
125 -- 152 |
MFS transporter |
|
_____________242__________________________271\n_____________|____________________________|\n5'-UGU...AAAUU_____U__U__C_____U__________AUACA...AGC-3'\n______________ACGUU_GC_UU_UUCGC_GCGGGUUUCA\n______________||:||_:|_||_|:|||_||::|||:||\n______________UGUAA_UG_AA_AGGCG_CGUUCAAGGU\n3'-AUU...UGUCC_____C__U__C_____U__________AUAUU...CAA-5'\n_____________|____________________________|\n_____________153__________________________124\n |
264 -- 270 |
125 -- 131 |
-24.436 kcal/mol |
4.92439 kcal/mol |
8.98549 kcal/mol |
242 271@LB@ | |@LB@5'-UGU...AAAUU U U C U AUACA...AGC-3'@LB@ ACGUU GC UU UUCGC GCGGGUUUCA@LB@ ||:|| :| || |:||| ||::|||:||@LB@ UGUAA UG AA AGGCG CGUUCAAGGU@LB@3'-AUU...UGUCC C U C U AUAUU...CAA-5'@LB@ | |@LB@ 153 124@LB@@LB@@LB@Energy -12.55 kcal/mol@LB@Hybridization Energy -24.436 kcal/mol@LB@Unfolding Energy - mRNA 4.92439 kcal/mol@LB@Unfolding Energy - sRNA 8.98549 kcal/mol@LB@@LB@Position - mRNA 243 -- 270@LB@Position - sRNA 125 -- 152@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 125 -- 131@LB@ |
| 128 |
0.005903 |
0.1741 |
bsu24110 |
yqzF |
-12.53 |
0.106013 |
93 -- 138 |
39 -- 81 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________92_______________________________________________139\n_____________|________________________________________________|\n5'-UAA...UAUUC_________AAUUU___C___CCUA___UA___CGUUUUCU_______ACAAA...UCA-3'\n______________CGUUGCAAU_____GCA_AAC____UGU__CCA________UCUCUUG\n______________|||||||||_____|||_|||____:||__|||________||||||:\n______________GCAACGUUA_____CGU_UUG____GCA__GGU________AGAGAAU\n3'-AUU...CCGAA_________GAAC________UUA____UG__________________AAGGG...CAA-5'\n_____________|________________________________________________|\n_____________82_______________________________________________38\n |
134 -- 140 |
45 -- 51 |
-26.5343 kcal/mol |
8.17713 kcal/mol |
8.56905 kcal/mol |
92 139@LB@ | |@LB@5'-UAA...UAUUC AAUUU C CCUA UA CGUUUUCU ACAAA...UCA-3'@LB@ CGUUGCAAU GCA AAC UGU CCA UCUCUUG@LB@ ||||||||| ||| ||| :|| ||| ||||||:@LB@ GCAACGUUA CGU UUG GCA GGU AGAGAAU@LB@3'-AUU...CCGAA GAAC UUA UG AAGGG...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -26.5343 kcal/mol@LB@Unfolding Energy - mRNA 8.17713 kcal/mol@LB@Unfolding Energy - sRNA 8.56905 kcal/mol@LB@@LB@Position - mRNA 93 -- 138@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 33 |
0.0009705 |
0.114 |
bsu20780 |
yopS |
-12.53 |
0.106187 |
15 -- 36 |
34 -- 54 |
XRE family transcriptional regulator |
|
_____________14_______________37\n_____________|________________|\n5'-GUU...ACCUG________C_______UAUGU...AAC-3'\n______________CCAUC_CU_AUUCUCC\n______________|||||_||_||||:||\n______________GGUAG_GA_UAAGGGG\n3'-AUU...GCAUG_____A__A_______UAAAG...CAA-5'\n_____________|________________|\n_____________55_______________33\n |
23 -- 29 |
39 -- 45 |
-17.4753 kcal/mol |
0.463886 kcal/mol |
7.9005 kcal/mol |
14 37@LB@ | |@LB@5'-GUU...ACCUG C UAUGU...AAC-3'@LB@ CCAUC CU AUUCUCC@LB@ ||||| || ||||:||@LB@ GGUAG GA UAAGGGG@LB@3'-AUU...GCAUG A A UAAAG...CAA-5'@LB@ | |@LB@ 55 33@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -17.4753 kcal/mol@LB@Unfolding Energy - mRNA 0.463886 kcal/mol@LB@Unfolding Energy - sRNA 7.9005 kcal/mol@LB@@LB@Position - mRNA 15 -- 36@LB@Position - sRNA 34 -- 54@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 177 |
0.008148 |
0.1751 |
bsu37320 |
narK |
-12.38 |
0.113512 |
147 -- 292 |
34 -- 178 |
nitrite extrusion protein |
|
_____________146________________________________________________________________________________________________________________________________________________________293\n_____________|__________________________________________________________________________________________________________________________________________________________|\n5'-CAA...UUGGC__G_______AU____A___UGAUU_____AU_____AAA_G___________A_____CAACC__CAA___A___AA_____G___C___CAUUAA______G__G___________UU______UGG_____G_________CA________CCAAAUCA-3'\n______________UA_UUUUUGU__GAUA_GAC_____AUUGA__CAUUG___G_GGCAAG__CCA_AUGAU_____GU___CAC_UUC__UUAUC_UUA_AGU________GCUU_GU_GCAG____GGU__AUGGUU___GUGCU_AUUUC__AU__CUCAUUUC\n______________||_|:||::|__:|:|_|||_____|||||__|||||___|_:|||||__|||_||:||_____||___|||_|||__|:|||_|:|_|||________||||_||_|||:____||:__|:::|:___||:|:_||:||__||__|:||||||\n______________AU_AGAAGUA__UUGU_CUG_____UAACU__GUAAC___C_UCGUUC__GGU_UAUUA_____CA___GUG_AAG__AGUAG_AGU_UCA________CGAA_CA_CGUU____CCG__UGUUAG___CAUGG_UAGAG__UA__GGGUAAAG\n3'-AUU...AAAAA__G_______G_____C____________________AGG_G______AA___A____________ACA___A__________A___U___UUACUUAC____G__A____AGAA___UU______________G_____AA__AG________AAACU...CAA-5'\n_____________|__________________________________________________________________________________________________________________________________________________________|\n_____________179________________________________________________________________________________________________________________________________________________________33\n |
286 -- 292 |
34 -- 40 |
-71.0788 kcal/mol |
32.1142 kcal/mol |
31.2836 kcal/mol |
146 293@LB@ | |@LB@5'-CAA...UUGGC G AU A UGAUU AU AAA G A CAACC CAA A AA G C CAUUAA G G UU UGG G CA CCAAAUCA-3'@LB@ UA UUUUUGU GAUA GAC AUUGA CAUUG G GGCAAG CCA AUGAU GU CAC UUC UUAUC UUA AGU GCUU GU GCAG GGU AUGGUU GUGCU AUUUC AU CUCAUUUC@LB@ || |:||::| :|:| ||| ||||| ||||| | :||||| ||| ||:|| || ||| ||| |:||| |:| ||| |||| || |||: ||: |:::|: ||:|: ||:|| || |:||||||@LB@ AU AGAAGUA UUGU CUG UAACU GUAAC C UCGUUC GGU UAUUA CA GUG AAG AGUAG AGU UCA CGAA CA CGUU CCG UGUUAG CAUGG UAGAG UA GGGUAAAG@LB@3'-AUU...AAAAA G G C AGG G AA A ACA A A U UUACUUAC G A AGAA UU G AA AG AAACU...CAA-5'@LB@ | |@LB@ 179 33@LB@@LB@@LB@Energy -12.38 kcal/mol@LB@Hybridization Energy -71.0788 kcal/mol@LB@Unfolding Energy - mRNA 32.1142 kcal/mol@LB@Unfolding Energy - sRNA 31.2836 kcal/mol@LB@@LB@Position - mRNA 147 -- 292@LB@Position - sRNA 34 -- 178@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 129 |
0.005948 |
0.1741 |
bsu13580 |
ykrV |
-12.37 |
0.114104 |
93 -- 132 |
31 -- 61 |
transaminase MtnE |
|
_____________92________________________________________133\n_____________|_________________________________________|\n5'-CAG...AAGAU________AUUU________UAACA_____AAUU_______ACUUU...ACG-3'\n______________AUCGU_CC____CAUCUUUU_____CCCCA____UUUCUUU\n______________|||||_||____|||||:||_____|||||____|||||||\n______________UAGCA_GG____GUAGAGAA_____GGGGU____AAAGAAA\n3'-AUU...UUUGU_____U______________UAA__________________CUUUA...CAA-5'\n_____________|_________________________________________|\n_____________62________________________________________30\n |
126 -- 132 |
31 -- 37 |
-24.5638 kcal/mol |
3.82436 kcal/mol |
10.4555 kcal/mol |
92 133@LB@ | |@LB@5'-CAG...AAGAU AUUU UAACA AAUU ACUUU...ACG-3'@LB@ AUCGU CC CAUCUUUU CCCCA UUUCUUU@LB@ ||||| || |||||:|| ||||| |||||||@LB@ UAGCA GG GUAGAGAA GGGGU AAAGAAA@LB@3'-AUU...UUUGU U UAA CUUUA...CAA-5'@LB@ | |@LB@ 62 30@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -24.5638 kcal/mol@LB@Unfolding Energy - mRNA 3.82436 kcal/mol@LB@Unfolding Energy - sRNA 10.4555 kcal/mol@LB@@LB@Position - mRNA 93 -- 132@LB@Position - sRNA 31 -- 61@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 141 |
0.006569 |
0.1741 |
bsu19520 |
yojA |
-12.26 |
0.119762 |
158 -- 180 |
120 -- 146 |
permease YojA |
bsu40070 |
_____________157_________________________________________________________181\n_____________|___________________________________________________________|\n5'-GGU...GCUUU__G________________GAAAAGCGCCAAA_________________AA________AACAC...UUU-3'\n______________CU_UUCAUC__UAGG_CAU_____________ACAUUGUC____GCGAG__UCAUAUAA\n______________||_||||||__:|||_|||_____________||||||||____||:||__:|||||||\n______________GA_AAGUAG__GUCC_GUA_____________UGUAACAG____CGUUC__GGUAUAUU\n3'-AUU...AAAAU__G______UU____U___AC___________________GCGU_____AA________ACAAC...CAA-5'\n_____________|___________________________________________________________|\n_____________147_________________________________________________________119\n |
174 -- 180 |
120 -- 126 |
-32.1826 kcal/mol |
10.1468 kcal/mol |
10.5645 kcal/mol |
157 181@LB@ | |@LB@5'-GGU...GCUUU G GAAAAGCGCCAAA AA AACAC...UUU-3'@LB@ CU UUCAUC UAGG CAU ACAUUGUC GCGAG UCAUAUAA@LB@ || |||||| :||| ||| |||||||| ||:|| :|||||||@LB@ GA AAGUAG GUCC GUA UGUAACAG CGUUC GGUAUAUU@LB@3'-AUU...AAAAU G UU U AC GCGU AA ACAAC...CAA-5'@LB@ | |@LB@ 147 119@LB@@LB@@LB@Energy -12.26 kcal/mol@LB@Hybridization Energy -32.1826 kcal/mol@LB@Unfolding Energy - mRNA 10.1468 kcal/mol@LB@Unfolding Energy - sRNA 10.5645 kcal/mol@LB@@LB@Position - mRNA 158 -- 180@LB@Position - sRNA 120 -- 146@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 89 |
0.003315 |
0.1443 |
bsu30460 |
ytrA |
-11.86 |
0.143485 |
75 -- 137 |
74 -- 137 |
GntR family transcriptional regulator |
|
_____________74__________________________________________________________________138\n_____________|___________________________________________________________________|\n5'-UUU...UGAUU_______________AUCGC_____CU_______U_G___UU________UC_______AAUG____AAAAG...AUA-3'\n______________GCGGCA___UCUGUG_____UUGUU__UUUUUUC_U_UUC___GUGAUGA__GGUUUUG____GCAA\n______________||:|||___||::|:_____||||:__:|||:||_|_|||___||:||||__||:||:|____||||\n______________CGUCGU___AGGUAU_____AACAG__GAAAGAG_A_AAG___CAUUACU__CCGAAGC____CGUU\n3'-AUU...ACAGG______UCA______AUUAC_____U________U_G___UUU_______UA_______AA______AGAAC...CAA-5'\n_____________|___________________________________________________________________|\n_____________138_________________________________________________________________73\n |
123 -- 129 |
80 -- 86 |
-41.3024 kcal/mol |
16.5538 kcal/mol |
14.8582 kcal/mol |
74 138@LB@ | |@LB@5'-UUU...UGAUU AUCGC CU U G UU UC AAUG AAAAG...AUA-3'@LB@ GCGGCA UCUGUG UUGUU UUUUUUC U UUC GUGAUGA GGUUUUG GCAA@LB@ ||:||| ||::|: ||||: :|||:|| | ||| ||:|||| ||:||:| ||||@LB@ CGUCGU AGGUAU AACAG GAAAGAG A AAG CAUUACU CCGAAGC CGUU@LB@3'-AUU...ACAGG UCA AUUAC U U G UUU UA AA AGAAC...CAA-5'@LB@ | |@LB@ 138 73@LB@@LB@@LB@Energy -11.86 kcal/mol@LB@Hybridization Energy -41.3024 kcal/mol@LB@Unfolding Energy - mRNA 16.5538 kcal/mol@LB@Unfolding Energy - sRNA 14.8582 kcal/mol@LB@@LB@Position - mRNA 75 -- 137@LB@Position - sRNA 74 -- 137@LB@Position Seed - mRNA 123 -- 129@LB@Position Seed - sRNA 80 -- 86@LB@ |
| 145 |
0.006739 |
0.1741 |
bsu12460 |
xlyB |
-11.80 |
0.146979 |
21 -- 64 |
155 -- 186 |
N-acetylmuramoyl-L-alanine amidase XlyB |
bsu12810 bsu25710 bsu25900 bsu21410 |
_____________20____________________________________65\n_____________|_____________________________________|\n5'-UCC...CCACA_____C_G_____AUUUCUC____CU_______A___UUAAA...CGG-3'\n______________UGCUC_U_AUUAA_______CGUU__CAUUGUC_GCA\n______________|:|||_|_||:||_______|:||__|||||||_|||\n______________AUGAG_A_UAGUU_______GUAA__GUAACAG_CGU\n3'-AUU...AAAAA_____A_G_____GUCCU______CU_______G___CGUUC...CAA-5'\n_____________|_____________________________________|\n_____________187___________________________________154\n |
54 -- 60 |
139 -- 145 |
-22.5299 kcal/mol |
4.58402 kcal/mol |
8.54622 kcal/mol |
20 65@LB@ | |@LB@5'-UCC...CCACA C G AUUUCUC CU A UUAAA...CGG-3'@LB@ UGCUC U AUUAA CGUU CAUUGUC GCA@LB@ |:||| | ||:|| |:|| ||||||| |||@LB@ AUGAG A UAGUU GUAA GUAACAG CGU@LB@3'-AUU...AAAAA A G GUCCU CU G CGUUC...CAA-5'@LB@ | |@LB@ 187 154@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -22.5299 kcal/mol@LB@Unfolding Energy - mRNA 4.58402 kcal/mol@LB@Unfolding Energy - sRNA 8.54622 kcal/mol@LB@@LB@Position - mRNA 21 -- 64@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 139 -- 145@LB@ |
| 51 |
0.001644 |
0.1212 |
bsu29870 |
ytzB |
-11.72 |
0.152375 |
23 -- 59 |
33 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________22__________________________________________60\n_____________|___________________________________________|\n5'-CUG...AUUGC___________________________________________AAAAG...AAG-3'\n______________UUAACAGGAU_UUGGC__UGUU__________UCUGUAUAAUG\n______________|:|||||||:_|||:|__|||:__________||::|||||||\n______________AGUUGUCCUG_AACUG__ACAG__________AGGUAUAUUAC\n3'-AUU...GAAGU__________U_____UA____GCGUCGUUCA___________AACAG...CAA-5'\n_____________|___________________________________________|\n_____________86__________________________________________32\n |
163 -- 169 |
118 -- 124 |
-27.3559 kcal/mol |
7.06665 kcal/mol |
10.6143 kcal/mol |
22 60@LB@ | |@LB@5'-CUG...AUUGC AAAAG...AAG-3'@LB@ UUAACAGGAU UUGGC UGUU UCUGUAUAAUG@LB@ |:|||||||: |||:| |||: ||::|||||||@LB@ AGUUGUCCUG AACUG ACAG AGGUAUAUUAC@LB@3'-AUU...GAAGU U UA GCGUCGUUCA AACAG...CAA-5'@LB@ | |@LB@ 86 32@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -27.3559 kcal/mol@LB@Unfolding Energy - mRNA 7.06665 kcal/mol@LB@Unfolding Energy - sRNA 10.6143 kcal/mol@LB@@LB@Position - mRNA 23 -- 59@LB@Position - sRNA 33 -- 85@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 118 -- 124@LB@ |
