| 167 |
0.007775 |
0.1751 |
bsu09889 |
yhzD |
-15.75 |
0.023145 |
43 -- 92 |
39 -- 89 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________42____________________________________________________93\n_____________|_____________________________________________________|\n5'-GGA...CUCCA____U_______AC______AAUU_______C___A____C____C_______UGACC...GUG-3'\n______________AAUG_CUUCGUU__GUGAUC____GUAAGCA__UG_AUCU__CUC___UUCCC\n______________||||_|||||||__|::|||____|:||:||__:|_|:|:__|||___|||||\n______________UUAC_GAAGCAA__CGUUAG____CGUUUGU__GC_UGGG__GAG___AAGGG\n3'-AUU...AUUAC____C_______________AAC________UA__A____UA___AAU_____GUAAA...CAA-5'\n_____________|_____________________________________________________|\n_____________90____________________________________________________38\n |
67 -- 73 |
62 -- 68 |
-30.4166 kcal/mol |
5.34264 kcal/mol |
10.926 kcal/mol |
42 93@LB@ | |@LB@5'-GGA...CUCCA U AC AAUU C A C C UGACC...GUG-3'@LB@ AAUG CUUCGUU GUGAUC GUAAGCA UG AUCU CUC UUCCC@LB@ |||| ||||||| |::||| |:||:|| :| |:|: ||| |||||@LB@ UUAC GAAGCAA CGUUAG CGUUUGU GC UGGG GAG AAGGG@LB@3'-AUU...AUUAC C AAC UA A UA AAU GUAAA...CAA-5'@LB@ | |@LB@ 90 38@LB@@LB@@LB@Energy -15.75 kcal/mol@LB@Hybridization Energy -30.4166 kcal/mol@LB@Unfolding Energy - mRNA 5.34264 kcal/mol@LB@Unfolding Energy - sRNA 10.926 kcal/mol@LB@@LB@Position - mRNA 43 -- 92@LB@Position - sRNA 39 -- 89@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 41 |
0.00131 |
0.1206 |
bsu01580 |
ybaR |
-15.69 |
0.023818 |
44 -- 97 |
34 -- 84 |
sulfate transporter YbaR |
|
_____________43________________________________________________________________98\n_____________|_________________________________________________________________|\n5'-CAG...UUAUU____UACU______A__A___CUC____CCU___U_____A_______A_GU_____________UCUCC...CGA-3'\n______________AUGA____CUUUGU_UG_CAA___CUUG___CAA_ACAAU__UACUCA_C_____UUCCCCGUUU\n______________||||____|||:||_||_|||___||||___|||_|||||__|||:||_|_____||||||:|||\n______________UACU____GAAGCA_AC_GUU___GAAC___GUU_UGUUA__AUGGGU_G_____AAGGGGUAAA\n3'-AUU...UUCAU____UACC_____________A______C___________GC______A_AGAAU__________GAAAC...CAA-5'\n_____________|_________________________________________________________________|\n_____________85________________________________________________________________33\n |
90 -- 96 |
35 -- 41 |
-34.7178 kcal/mol |
7.59764 kcal/mol |
14.4179 kcal/mol |
43 98@LB@ | |@LB@5'-CAG...UUAUU UACU A A CUC CCU U A A GU UCUCC...CGA-3'@LB@ AUGA CUUUGU UG CAA CUUG CAA ACAAU UACUCA C UUCCCCGUUU@LB@ |||| |||:|| || ||| |||| ||| ||||| |||:|| | ||||||:|||@LB@ UACU GAAGCA AC GUU GAAC GUU UGUUA AUGGGU G AAGGGGUAAA@LB@3'-AUU...UUCAU UACC A C GC A AGAAU GAAAC...CAA-5'@LB@ | |@LB@ 85 33@LB@@LB@@LB@Energy -15.69 kcal/mol@LB@Hybridization Energy -34.7178 kcal/mol@LB@Unfolding Energy - mRNA 7.59764 kcal/mol@LB@Unfolding Energy - sRNA 14.4179 kcal/mol@LB@@LB@Position - mRNA 44 -- 97@LB@Position - sRNA 34 -- 84@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 198 |
0.009004 |
0.1751 |
bsu38400 |
epr |
-15.65 |
0.024309 |
91 -- 136 |
32 -- 78 |
minor extracellular protease epr |
|
_____________90________________________________________________137\n_____________|_________________________________________________|\n5'-UGU...AAAUC__________C_______C_C_______GAAAAAUU_____________ACUAU...AAG-3'\n______________UGC_AUUUUG_UAAACAA_C_UGCCCAU________UUUUCC__UUCUU\n______________|||_||:|||_:||||||_|_|:|||||________||::||__|||||\n______________ACG_UAGAAC_GUUUGUU_G_AUGGGUA________AAGGGG__AAGAA\n3'-AUU...AAGCA___U______C_______A_C_______GAGAAU________UA_____ACUUU...CAA-5'\n_____________|_________________________________________________|\n_____________79________________________________________________31\n |
111 -- 117 |
51 -- 57 |
-30.7525 kcal/mol |
4.20051 kcal/mol |
13.8985 kcal/mol |
90 137@LB@ | |@LB@5'-UGU...AAAUC C C C GAAAAAUU ACUAU...AAG-3'@LB@ UGC AUUUUG UAAACAA C UGCCCAU UUUUCC UUCUU@LB@ ||| ||:||| :|||||| | |:||||| ||::|| |||||@LB@ ACG UAGAAC GUUUGUU G AUGGGUA AAGGGG AAGAA@LB@3'-AUU...AAGCA U C A C GAGAAU UA ACUUU...CAA-5'@LB@ | |@LB@ 79 31@LB@@LB@@LB@Energy -15.65 kcal/mol@LB@Hybridization Energy -30.7525 kcal/mol@LB@Unfolding Energy - mRNA 4.20051 kcal/mol@LB@Unfolding Energy - sRNA 13.8985 kcal/mol@LB@@LB@Position - mRNA 91 -- 136@LB@Position - sRNA 32 -- 78@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 51 -- 57@LB@ |
| 190 |
0.008698 |
0.1751 |
bsu19130 |
yocA |
-15.61 |
0.024807 |
4 -- 94 |
40 -- 127 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type h: extrachromosomal origin' |
|
_____________3___________________________________________________________________________________________________95\n_____________|___________________________________________________________________________________________________|\n________5'-CAG_______UA_______C_______UA_____U___UUUAUCUUAUAACAAA__________AGU______CA_________________A_________AAUCU...ACC-3'\n______________UCAUAUG______UCA_CUUUCUU___UUCA_AGU________________UGCG__CUUG___GCAAAC______GCCUG___CUUGU_UUCCAUUUC\n______________:|||||:______|||_||||||:___||||_|||________________|||:__||||___||||||______:||::___|||:|_::|||||||\n______________GGUAUAU______AGU_GAAAGAG___AAGU_UCA________________ACGU__GAAC___CGUUUG______UGGGU___GAAUA_GGGGUAAAG\n3'-AUU...UUCAA_______UACAAC___________UAG____U___UUACUUACCGAAGCA_____UA_____________UUAGCA_____AGA_____A_________AAACU...CAA-5'\n_____________|___________________________________________________________________________________________________|\n_____________128_________________________________________________________________________________________________39\n |
82 -- 88 |
34 -- 40 |
-43.0073 kcal/mol |
9.62595 kcal/mol |
23.221 kcal/mol |
3 95@LB@ | |@LB@ 5'-CAG UA C UA U UUUAUCUUAUAACAAA AGU CA A AAUCU...ACC-3'@LB@ UCAUAUG UCA CUUUCUU UUCA AGU UGCG CUUG GCAAAC GCCUG CUUGU UUCCAUUUC@LB@ :|||||: ||| ||||||: |||| ||| |||: |||| |||||| :||:: |||:| ::|||||||@LB@ GGUAUAU AGU GAAAGAG AAGU UCA ACGU GAAC CGUUUG UGGGU GAAUA GGGGUAAAG@LB@3'-AUU...UUCAA UACAAC UAG U UUACUUACCGAAGCA UA UUAGCA AGA A AAACU...CAA-5'@LB@ | |@LB@ 128 39@LB@@LB@@LB@Energy -15.61 kcal/mol@LB@Hybridization Energy -43.0073 kcal/mol@LB@Unfolding Energy - mRNA 9.62595 kcal/mol@LB@Unfolding Energy - sRNA 23.221 kcal/mol@LB@@LB@Position - mRNA 4 -- 94@LB@Position - sRNA 40 -- 127@LB@Position Seed - mRNA 82 -- 88@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 132 |
0.006133 |
0.1741 |
bsu24800 |
yqgW |
-15.55 |
0.025469 |
1 -- 63 |
31 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
______________1_________________________________________________________________64\n______________|_________________________________________________________________|\n___________5'-__G____C_____________CCUACU____U____C________ACUA____CA___C_______UUUAU...CCG-3'\n______________GC_UUCG_UUG_AAUC__GGC______GGCA_UCGU_UCCAUUUC____UUAU__CCU_UUUCUUU\n______________||_||||_|||_||||__|||______::||_||||_:||||:||____||||__||:_|||||||\n______________CG_AAGC_AAC_UUAG__CCG______UUGU_AGCA_GGGUAGAG____AAUA__GGG_AAAGAAA\n3'-AUU...CUUAC___________G____AA___U_________U____U________________AG___U_______CUUUA...CAA-5'\n_____________|__________________________________________________________________|\n_____________86_________________________________________________________________30\n |
36 -- 42 |
48 -- 54 |
-38.6272 kcal/mol |
10.4973 kcal/mol |
14.833 kcal/mol |
1 64@LB@ | |@LB@ 5'- G C CCUACU U C ACUA CA C UUUAU...CCG-3'@LB@ GC UUCG UUG AAUC GGC GGCA UCGU UCCAUUUC UUAU CCU UUUCUUU@LB@ || |||| ||| |||| ||| ::|| |||| :||||:|| |||| ||: |||||||@LB@ CG AAGC AAC UUAG CCG UUGU AGCA GGGUAGAG AAUA GGG AAAGAAA@LB@3'-AUU...CUUAC G AA U U U AG U CUUUA...CAA-5'@LB@ | |@LB@ 86 30@LB@@LB@@LB@Energy -15.55 kcal/mol@LB@Hybridization Energy -38.6272 kcal/mol@LB@Unfolding Energy - mRNA 10.4973 kcal/mol@LB@Unfolding Energy - sRNA 14.833 kcal/mol@LB@@LB@Position - mRNA 1 -- 63@LB@Position - sRNA 31 -- 85@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 32 |
0.0008744 |
0.1059 |
bsu09430 |
citR |
-15.39 |
0.027619 |
23 -- 103 |
39 -- 111 |
LysR family transcriptional regulator |
|
_____________22______________________________________________________________________________________104\n_____________|_______________________________________________________________________________________|\n5'-UGU...ACGCC________C_A____UAU__C______________UACACA_______CCCUU________CGUAAU__________G_________CUAUC...CCG-3'\n______________CUUUUUCA_C_UCAA___GG_UGAUGGAU_GUUUC______UGUAAUU________UAAUC______GUA_CCAUUU__UAUUCCCC\n______________||||:|||_|_||||___:|_|:|||:||_|:|||______||:|||:________:||||______|||_||||:|__||||||||\n______________GAAAGAGU_G_AGUU___UC_AUUACUUA_CGAAG______ACGUUAG________GUUAG______CAU_GGUAGA__AUAAGGGG\n3'-AUU...ACAGU________A_A__________________C_____CA___________AACCGUUU______________G______GA________UAAAG...CAA-5'\n_____________|_______________________________________________________________________________________|\n_____________112_____________________________________________________________________________________38\n |
97 -- 103 |
39 -- 45 |
-43.1846 kcal/mol |
11.0009 kcal/mol |
20.821 kcal/mol |
22 104@LB@ | |@LB@5'-UGU...ACGCC C A UAU C UACACA CCCUU CGUAAU G CUAUC...CCG-3'@LB@ CUUUUUCA C UCAA GG UGAUGGAU GUUUC UGUAAUU UAAUC GUA CCAUUU UAUUCCCC@LB@ ||||:||| | |||| :| |:|||:|| |:||| ||:|||: :|||| ||| ||||:| ||||||||@LB@ GAAAGAGU G AGUU UC AUUACUUA CGAAG ACGUUAG GUUAG CAU GGUAGA AUAAGGGG@LB@3'-AUU...ACAGU A A C CA AACCGUUU G GA UAAAG...CAA-5'@LB@ | |@LB@ 112 38@LB@@LB@@LB@Energy -15.39 kcal/mol@LB@Hybridization Energy -43.1846 kcal/mol@LB@Unfolding Energy - mRNA 11.0009 kcal/mol@LB@Unfolding Energy - sRNA 20.821 kcal/mol@LB@@LB@Position - mRNA 23 -- 103@LB@Position - sRNA 39 -- 111@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 97 |
0.003858 |
0.1541 |
bsu32520 |
pucG |
-15.24 |
0.029596 |
117 -- 232 |
36 -- 162 |
purine catabolism protein PucG |
|
_____________116_____________________________________________________________________________________________________________________________________233\n_____________|_______________________________________________________________________________________________________________________________________|\n5'-UGA...AUAUA_______CA____GC____G__CGGC__________CUGA__GAUUUAUUAUACAA___GG_______GA_____GAG_________GU_________A_______A__GAAU___G__________________AAGAA...CGG-3'\n______________CGUCAGC__GACA__UUGA_AU____GUCCGC_GCA____CU______________ACU__CUUA_CU__UAAAG____GAAUGGCU________GUC__GGCAGA_GA____UAU_CA_________CCCCGUU\n______________|:|||:|__||||__||||_||____||||||_|||____|:______________|||__||:|_||__:||||____||||||||________:||__||||:|_:|____||:_||_________||||:||\n______________GUAGUUG__CUGU__AACU_UA____CAGGCG_CGU____GG______________UGA__GAGU_GA__GUUUC____CUUACCGA________UAG__CCGUUU_UU____AUG_GU_________GGGGUAA\n3'-AUU...GAGAA_______UC__________G__A_________U___UCAA__UAUAUUACAACAG____AA____A__A______AUUA________AGCAACGU___AA______G__AGC____G__AGAGAAUAA_______AGAAA...CAA-5'\n_____________|_______________________________________________________________________________________________________________________________________|\n_____________163_____________________________________________________________________________________________________________________________________35\n |
226 -- 232 |
36 -- 42 |
-68.3253 kcal/mol |
25.8271 kcal/mol |
31.2699 kcal/mol |
116 233@LB@ | |@LB@5'-UGA...AUAUA CA GC G CGGC CUGA GAUUUAUUAUACAA GG GA GAG GU A A GAAU G AAGAA...CGG-3'@LB@ CGUCAGC GACA UUGA AU GUCCGC GCA CU ACU CUUA CU UAAAG GAAUGGCU GUC GGCAGA GA UAU CA CCCCGUU@LB@ |:|||:| |||| |||| || |||||| ||| |: ||| ||:| || :|||| |||||||| :|| ||||:| :| ||: || ||||:||@LB@ GUAGUUG CUGU AACU UA CAGGCG CGU GG UGA GAGU GA GUUUC CUUACCGA UAG CCGUUU UU AUG GU GGGGUAA@LB@3'-AUU...GAGAA UC G A U UCAA UAUAUUACAACAG AA A A AUUA AGCAACGU AA G AGC G AGAGAAUAA AGAAA...CAA-5'@LB@ | |@LB@ 163 35@LB@@LB@@LB@Energy -15.24 kcal/mol@LB@Hybridization Energy -68.3253 kcal/mol@LB@Unfolding Energy - mRNA 25.8271 kcal/mol@LB@Unfolding Energy - sRNA 31.2699 kcal/mol@LB@@LB@Position - mRNA 117 -- 232@LB@Position - sRNA 36 -- 162@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 79 |
0.002683 |
0.1316 |
bsu12250 |
yjkB |
-15.23 |
0.029777 |
154 -- 245 |
42 -- 131 |
ABC transporter ATP-binding protein |
|
_____________153__________________________________________________________246\n_____________|____________________________________________________________|\n5'-AUA...UGAAA___GA__A_______GGAA__________AAACACAAAACAUACC_G_______C_____AGAUC...AGG-3'\n______________GGU__UU_UUGUAAU____GCAAACGAUC________________A_CCAUCUC___UUC\n______________||:__|:_|||:|||____||||||:|||________________|_|||||||___|||\n______________CCG__AG_AACGUUA____CGUUUGUUAG________________U_GGUAGAG___AAG\n3'-AUU...ACUUA___A___C_______GAAC__________CA_______________G_______AAU___GGGUA...CAA-5'\n_____________|____________________________________________________________|\n_____________132__________________________________________________________41\n |
235 -- 241 |
48 -- 54 |
-30.736 kcal/mol |
10.3673 kcal/mol |
9.19615 kcal/mol |
153 246@LB@ | |@LB@5'-AUA...UGAAA GA A GGAA AAACACAAAACAUACC G C AGAUC...AGG-3'@LB@ GGU UU UUGUAAU GCAAACGAUC A CCAUCUC UUC@LB@ ||: |: |||:||| ||||||:||| | ||||||| |||@LB@ CCG AG AACGUUA CGUUUGUUAG U GGUAGAG AAG@LB@3'-AUU...ACUUA A C GAAC CA G AAU GGGUA...CAA-5'@LB@ | |@LB@ 132 41@LB@@LB@@LB@Energy -15.23 kcal/mol@LB@Hybridization Energy -30.736 kcal/mol@LB@Unfolding Energy - mRNA 10.3673 kcal/mol@LB@Unfolding Energy - sRNA 9.19615 kcal/mol@LB@@LB@Position - mRNA 154 -- 245@LB@Position - sRNA 42 -- 131@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 156 |
0.007194 |
0.1751 |
bsu12900 |
htrA |
-15.16 |
0.030869 |
26 -- 118 |
38 -- 130 |
serine protease Do-like HtrA |
|
_____________25_______________________________________________________________________________________________________119\n_____________|________________________________________________________________________________________________________|\n5'-CUG...AAUCU_______CGACUCA__________A___A_____U________GU__AC____UUCC___C____UC_________U_______AA____UUCAUUUUUA____ACAGU...AUG-3'\n______________GUUCCAU________GUC_CUUUC_UAU___CAA____AUGAA__GU__CGUU____GCA_UUUU_____ACAAUU___CCCAU__UCUU__________UCCC\n______________|||||||________|||_|||||_:||___|||____|||||__|:__||||____|||_|:||_____|||||:___|||||__||||__________||||\n______________CAAGGUA________CAG_GAAAG_GUA___GUU____UACUU__CG__GCAA____CGU_AGAA_____UGUUAG___GGGUA__AGAA__________AGGG\n3'-AUU...UCGUU_______UAUUACAA___U_____A___GAA___UCAU_____AC__AA___________U____CCGUU______CAU_____G_____UA____________GUAAA...CAA-5'\n_____________|________________________________________________________________________________________________________|\n_____________131______________________________________________________________________________________________________37\n |
26 -- 32 |
124 -- 130 |
-40.6941 kcal/mol |
8.96571 kcal/mol |
22.8172 kcal/mol |
25 119@LB@ | |@LB@5'-CUG...AAUCU CGACUCA A A U GU AC UUCC C UC U AA UUCAUUUUUA ACAGU...AUG-3'@LB@ GUUCCAU GUC CUUUC UAU CAA AUGAA GU CGUU GCA UUUU ACAAUU CCCAU UCUU UCCC@LB@ ||||||| ||| ||||| :|| ||| ||||| |: |||| ||| |:|| |||||: ||||| |||| ||||@LB@ CAAGGUA CAG GAAAG GUA GUU UACUU CG GCAA CGU AGAA UGUUAG GGGUA AGAA AGGG@LB@3'-AUU...UCGUU UAUUACAA U A GAA UCAU AC AA U CCGUU CAU G UA GUAAA...CAA-5'@LB@ | |@LB@ 131 37@LB@@LB@@LB@Energy -15.16 kcal/mol@LB@Hybridization Energy -40.6941 kcal/mol@LB@Unfolding Energy - mRNA 8.96571 kcal/mol@LB@Unfolding Energy - sRNA 22.8172 kcal/mol@LB@@LB@Position - mRNA 26 -- 118@LB@Position - sRNA 38 -- 130@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 124 -- 130@LB@ |
| 76 |
0.002466 |
0.1257 |
bsu07870 |
yfkK |
-15.12 |
0.031390 |
12 -- 33 |
33 -- 55 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________11______________________34\n_____________|_______________________|\n5'-UGU...ACCUC__________UGC__________CUAAA...UCA-3'\n______________CCUA_UUCUU___CCCUAUUUCU\n______________||:|_:||||___|||:||||||\n______________GGGU_GAGAA___GGGGUAAAGA\n3'-AUU...AGCAU____A_____UAA__________AACUU...CAA-5'\n_____________|_______________________|\n_____________56______________________32\n |
27 -- 33 |
33 -- 39 |
-21.8682 kcal/mol |
0.689294 kcal/mol |
9.04338 kcal/mol |
11 34@LB@ | |@LB@5'-UGU...ACCUC UGC CUAAA...UCA-3'@LB@ CCUA UUCUU CCCUAUUUCU@LB@ ||:| :|||| |||:||||||@LB@ GGGU GAGAA GGGGUAAAGA@LB@3'-AUU...AGCAU A UAA AACUU...CAA-5'@LB@ | |@LB@ 56 32@LB@@LB@@LB@Energy -15.12 kcal/mol@LB@Hybridization Energy -21.8682 kcal/mol@LB@Unfolding Energy - mRNA 0.689294 kcal/mol@LB@Unfolding Energy - sRNA 9.04338 kcal/mol@LB@@LB@Position - mRNA 12 -- 33@LB@Position - sRNA 33 -- 55@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 33 -- 39@LB@ |
