| 137 |
0.006339 |
0.1741 |
bsu39320 |
yxiB |
-11.20 |
0.191268 |
188 -- 222 |
111 -- 147 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________187_________________________223\n_____________|___________________________|\n5'-UAA...GUGUU______UGCAUA___A___________AUUCG...AAA-3'\n______________GCGGCA______GUG_AAUGUUGUUAC\n______________||:|||______:|:_||||||||:||\n______________CGUCGU______UAU_UUACAACAGUG\n3'-AUU...ACAGG______UCAAGG___A___________AAAGA...CAA-5'\n_____________|___________________________|\n_____________148_________________________110\n |
216 -- 222 |
111 -- 117 |
-23.3412 kcal/mol |
7.76229 kcal/mol |
5.28835 kcal/mol |
187 223@LB@ | |@LB@5'-UAA...GUGUU UGCAUA A AUUCG...AAA-3'@LB@ GCGGCA GUG AAUGUUGUUAC@LB@ ||:||| :|: ||||||||:||@LB@ CGUCGU UAU UUACAACAGUG@LB@3'-AUU...ACAGG UCAAGG A AAAGA...CAA-5'@LB@ | |@LB@ 148 110@LB@@LB@@LB@Energy -11.20 kcal/mol@LB@Hybridization Energy -23.3412 kcal/mol@LB@Unfolding Energy - mRNA 7.76229 kcal/mol@LB@Unfolding Energy - sRNA 5.28835 kcal/mol@LB@@LB@Position - mRNA 188 -- 222@LB@Position - sRNA 111 -- 147@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 170 |
0.007871 |
0.1751 |
bsu13520 |
ykrP |
-11.14 |
0.196181 |
76 -- 136 |
99 -- 167 |
membrane-bound acyltransferase YkrP |
|
_____________75____________137\n_____________|_____________|\n5'-GGA...CCGUC_____A_______CUAAC...AUC-3'\n______________ACUUU_CAUCGAC\n______________|||:|_||||:||\n______________UGAGA_GUAGUUG\n3'-AUU...AAAAA_____A_______UCCUG...CAA-5'\n_____________|_____________|\n_____________168___________98\n |
91 -- 97 |
156 -- 162 |
-12.1838 kcal/mol |
0.966795 kcal/mol |
1.40592 kcal/mol |
75 137@LB@ | |@LB@5'-GGA...CCGUC A CUAAC...AUC-3'@LB@ ACUUU CAUCGAC@LB@ |||:| ||||:||@LB@ UGAGA GUAGUUG@LB@3'-AUU...AAAAA A UCCUG...CAA-5'@LB@ | |@LB@ 168 98@LB@@LB@@LB@Energy -11.14 kcal/mol@LB@Hybridization Energy -12.1838 kcal/mol@LB@Unfolding Energy - mRNA 0.966795 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 76 -- 136@LB@Position - sRNA 99 -- 167@LB@Position Seed - mRNA 91 -- 97@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 54 |
0.001793 |
0.1212 |
bsu21030 |
yonP |
-11.11 |
0.198660 |
20 -- 36 |
161 -- 178 |
Evidence 5: No homology to any previously reported sequences |
|
_____________19_______37\n_____________|________|\n5'-AAU...UUUAA________AUAUU...ACA-3'\n______________ACUCUUUA\n______________||||||:|\n______________UGAGAAGU\n3'-AUU...AAAAA________AGUUG...CAA-5'\n_____________|________|\n_____________179______160\n |
30 -- 36 |
161 -- 167 |
-8.6372 kcal/mol |
0.252156 kcal/mol |
0.576912 kcal/mol |
19 37@LB@ | |@LB@5'-AAU...UUUAA AUAUU...ACA-3'@LB@ ACUCUUUA@LB@ ||||||:|@LB@ UGAGAAGU@LB@3'-AUU...AAAAA AGUUG...CAA-5'@LB@ | |@LB@ 179 160@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -8.6372 kcal/mol@LB@Unfolding Energy - mRNA 0.252156 kcal/mol@LB@Unfolding Energy - sRNA 0.576912 kcal/mol@LB@@LB@Position - mRNA 20 -- 36@LB@Position - sRNA 161 -- 178@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 161 -- 167@LB@ |
| 186 |
0.00854 |
0.1751 |
bsu03320 |
nasB |
-11.11 |
0.199178 |
25 -- 36 |
33 -- 45 |
assimilatory nitrate reductase electron transfer subunit |
bsu03300 |
_____________24____________37\n_____________|_____________|\n5'-UUU...AUAAG_____________AGACC...UCA-3'\n______________AUUCCCC_UUUCU\n______________|||||||_|||||\n______________UAAGGGG_AAAGA\n3'-AUU...GAGAA_______U_____AACUU...CAA-5'\n_____________|_____________|\n_____________46____________32\n |
25 -- 31 |
39 -- 45 |
-15.5675 kcal/mol |
1.76448 kcal/mol |
4.94568 kcal/mol |
24 37@LB@ | |@LB@5'-UUU...AUAAG AGACC...UCA-3'@LB@ AUUCCCC UUUCU@LB@ ||||||| |||||@LB@ UAAGGGG AAAGA@LB@3'-AUU...GAGAA U AACUU...CAA-5'@LB@ | |@LB@ 46 32@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -15.5675 kcal/mol@LB@Unfolding Energy - mRNA 1.76448 kcal/mol@LB@Unfolding Energy - sRNA 4.94568 kcal/mol@LB@@LB@Position - mRNA 25 -- 36@LB@Position - sRNA 33 -- 45@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 40 |
0.001293 |
0.1206 |
bsu13810 |
ykvS |
-11.02 |
0.206841 |
41 -- 57 |
31 -- 45 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________40________________58\n_____________|_________________|\n5'-UGU...CAAAA_______CCU_______CUAUU...CGA-3'\n______________AUUCCCC___UUUCUUU\n______________|||||||___|||||||\n______________UAAGGGG___AAAGAAA\n3'-AUU...GAGAA_______U_________CUUUA...CAA-5'\n_____________|_________________|\n_____________46________________30\n |
51 -- 57 |
31 -- 37 |
-15.5048 kcal/mol |
1.25691 kcal/mol |
5.1304 kcal/mol |
40 58@LB@ | |@LB@5'-UGU...CAAAA CCU CUAUU...CGA-3'@LB@ AUUCCCC UUUCUUU@LB@ ||||||| |||||||@LB@ UAAGGGG AAAGAAA@LB@3'-AUU...GAGAA U CUUUA...CAA-5'@LB@ | |@LB@ 46 30@LB@@LB@@LB@Energy -11.02 kcal/mol@LB@Hybridization Energy -15.5048 kcal/mol@LB@Unfolding Energy - mRNA 1.25691 kcal/mol@LB@Unfolding Energy - sRNA 5.1304 kcal/mol@LB@@LB@Position - mRNA 41 -- 57@LB@Position - sRNA 31 -- 45@LB@Position Seed - mRNA 51 -- 57@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 125 |
0.005817 |
0.1741 |
bsu26980 |
yraE |
-11.00 |
0.208718 |
35 -- 90 |
112 -- 185 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________34________________________________________________________91\n_____________|_________________________________________________________|\n5'-UAC...UUUGA___AG______________AA_______A____GGA_____________________AGCCA...CUG-3'\n______________ACU___UAUCGACAGG_CA_____AUUG_UCCG_______________GAUGUUGUC\n______________|||___:|||:|||||_||_____||||_||||_______________:||||||||\n______________UGA___GUAGUUGUCC_GU_____UAAC_AGGC_______________UUACAACAG\n3'-AUU...AAAAA___GAA__________U__AACUG_________GUCGUUCAAGGUAUA_________UGAAA...CAA-5'\n_____________|_________________________________________________________|\n_____________186_______________________________________________________111\n |
83 -- 89 |
113 -- 119 |
-30.5681 kcal/mol |
8.54797 kcal/mol |
10.8348 kcal/mol |
34 91@LB@ | |@LB@5'-UAC...UUUGA AG AA A GGA AGCCA...CUG-3'@LB@ ACU UAUCGACAGG CA AUUG UCCG GAUGUUGUC@LB@ ||| :|||:||||| || |||| |||| :||||||||@LB@ UGA GUAGUUGUCC GU UAAC AGGC UUACAACAG@LB@3'-AUU...AAAAA GAA U AACUG GUCGUUCAAGGUAUA UGAAA...CAA-5'@LB@ | |@LB@ 186 111@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -30.5681 kcal/mol@LB@Unfolding Energy - mRNA 8.54797 kcal/mol@LB@Unfolding Energy - sRNA 10.8348 kcal/mol@LB@@LB@Position - mRNA 35 -- 90@LB@Position - sRNA 112 -- 185@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 113 -- 119@LB@ |
| 131 |
0.006003 |
0.1741 |
bsu37510 |
pbpG |
-10.90 |
0.216982 |
70 -- 106 |
84 -- 108 |
penicillin-binding protein 2D |
|
_____________69____________________________________107\n_____________|_____________________________________|\n5'-UGU...UGAAA___________CCUAAGGAAA_____UAGA_______GGGGA...UGU-3'\n______________UCUCAUUUUCA__________AAGUA____GGAUGGC\n______________||||||:||||__________|||||____|:|||||\n______________AGAGUAGAAGU__________UUCAU____CUUACCG\n3'-AUU...GUGAA__________________________UA_________AAGCA...CAA-5'\n_____________|_____________________________________|\n_____________109___________________________________83\n |
100 -- 106 |
84 -- 90 |
-24.2284 kcal/mol |
7.40636 kcal/mol |
9.09036 kcal/mol |
69 107@LB@ | |@LB@5'-UGU...UGAAA CCUAAGGAAA UAGA GGGGA...UGU-3'@LB@ UCUCAUUUUCA AAGUA GGAUGGC@LB@ ||||||:|||| ||||| |:|||||@LB@ AGAGUAGAAGU UUCAU CUUACCG@LB@3'-AUU...GUGAA UA AAGCA...CAA-5'@LB@ | |@LB@ 109 83@LB@@LB@@LB@Energy -10.90 kcal/mol@LB@Hybridization Energy -24.2284 kcal/mol@LB@Unfolding Energy - mRNA 7.40636 kcal/mol@LB@Unfolding Energy - sRNA 9.09036 kcal/mol@LB@@LB@Position - mRNA 70 -- 106@LB@Position - sRNA 84 -- 108@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 84 -- 90@LB@ |
| 105 |
0.004352 |
0.1606 |
bsu05340 |
ydfA |
-10.81 |
0.225539 |
212 -- 245 |
38 -- 75 |
arsenic pump protein |
|
_____________211____________________________246\n_____________|______________________________|\n5'-AGC...UCAAU______________________________UAUUA...GAG-3'\n______________GAUUUUGGCA_______GUAUUCAUCUUUU\n______________:||:||||||_______|||::|||||:||\n______________UUAGAACCGU_______CAUGGGUAGAGAA\n3'-AUU...CAACG__________UUGUUAG_____________UAAGG...CAA-5'\n_____________|______________________________|\n_____________76_____________________________37\n |
227 -- 233 |
47 -- 53 |
-24.4224 kcal/mol |
4.10196 kcal/mol |
8.49986 kcal/mol |
211 246@LB@ | |@LB@5'-AGC...UCAAU UAUUA...GAG-3'@LB@ GAUUUUGGCA GUAUUCAUCUUUU@LB@ :||:|||||| |||::|||||:||@LB@ UUAGAACCGU CAUGGGUAGAGAA@LB@3'-AUU...CAACG UUGUUAG UAAGG...CAA-5'@LB@ | |@LB@ 76 37@LB@@LB@@LB@Energy -10.81 kcal/mol@LB@Hybridization Energy -24.4224 kcal/mol@LB@Unfolding Energy - mRNA 4.10196 kcal/mol@LB@Unfolding Energy - sRNA 8.49986 kcal/mol@LB@@LB@Position - mRNA 212 -- 245@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 133 |
0.006226 |
0.1741 |
bsu14430 |
ykpA |
-10.78 |
0.228396 |
48 -- 105 |
75 -- 129 |
ABC transporter ATP-binding protein |
|
_____________47____________106\n_____________|_____________|\n5'-GUA...AAGUU_____________AAUAU...UUG-3'\n______________AUUUGAAGAUGUU\n______________|||||||:|:|:|\n______________UAAACUUUUGCGA\n3'-AUU...AACUU_____________AUUAA...CAA-5'\n_____________|_____________|\n_____________130___________74\n |
254 -- 260 |
15 -- 21 |
-11.9489 kcal/mol |
0.844332 kcal/mol |
3.96844 kcal/mol |
47 106@LB@ | |@LB@5'-GUA...AAGUU AAUAU...UUG-3'@LB@ AUUUGAAGAUGUU@LB@ |||||||:|:|:|@LB@ UAAACUUUUGCGA@LB@3'-AUU...AACUU AUUAA...CAA-5'@LB@ | |@LB@ 130 74@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -11.9489 kcal/mol@LB@Unfolding Energy - mRNA 0.844332 kcal/mol@LB@Unfolding Energy - sRNA 3.96844 kcal/mol@LB@@LB@Position - mRNA 48 -- 105@LB@Position - sRNA 75 -- 129@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 62 |
0.001958 |
0.1214 |
bsu25150 |
yqfQ |
-10.78 |
0.228520 |
210 -- 221 |
125 -- 139 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________209_____________222\n_____________|_______________|\n5'-AAU...UUUCA_______________AUGAG...UGC-3'\n______________CCGCAGCAG___CCA\n______________||||||||:___|||\n______________GGCGUCGUU___GGU\n3'-AUU...UAACA_________CAA___AUAUU...CAA-5'\n_____________|_______________|\n_____________140_____________124\n |
212 -- 218 |
131 -- 137 |
-17.5181 kcal/mol |
2.74724 kcal/mol |
6.06036 kcal/mol |
209 222@LB@ | |@LB@5'-AAU...UUUCA AUGAG...UGC-3'@LB@ CCGCAGCAG CCA@LB@ ||||||||: |||@LB@ GGCGUCGUU GGU@LB@3'-AUU...UAACA CAA AUAUU...CAA-5'@LB@ | |@LB@ 140 124@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -17.5181 kcal/mol@LB@Unfolding Energy - mRNA 2.74724 kcal/mol@LB@Unfolding Energy - sRNA 6.06036 kcal/mol@LB@@LB@Position - mRNA 210 -- 221@LB@Position - sRNA 125 -- 139@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 131 -- 137@LB@ |
