| 23 |
0.0006135 |
0.1011 |
bsu23610 |
nudF |
-13.21 |
0.077687 |
6 -- 79 |
37 -- 88 |
adp-ribose pyrophosphatase |
|
_____________5__________________________________________________________________________80\n_____________|__________________________________________________________________________|\n______5'-CUUUU_________AGAUC_______UCA__UAUUG__A_____GU____A_____UUAACUUCGCUCGA_________GCUUU...AAG-3'\n______________AUGGCUUUG_____CAAUCUU___CA_____AA_UGUGC__CCAU_CUCUU______________AUUUCCCAU\n______________|||||||:|_____|||||||___||_____||_:||:|__||||_|||||______________|||:|||||\n______________UACCGAAGC_____GUUAGAA___GU_____UU_GCAUG__GGUA_GAGAA______________UAAGGGGUA\n3'-AUU...UUACU_________AAC_________CC___UUG____A________________________________________AAGAA...CAA-5'\n_____________|__________________________________________________________________________|\n_____________89_________________________________________________________________________36\n |
73 -- 79 |
37 -- 43 |
-36.6547 kcal/mol |
11.779 kcal/mol |
14.1528 kcal/mol |
5 80@LB@ | |@LB@ 5'-CUUUU AGAUC UCA UAUUG A GU A UUAACUUCGCUCGA GCUUU...AAG-3'@LB@ AUGGCUUUG CAAUCUU CA AA UGUGC CCAU CUCUU AUUUCCCAU@LB@ |||||||:| ||||||| || || :||:| |||| ||||| |||:|||||@LB@ UACCGAAGC GUUAGAA GU UU GCAUG GGUA GAGAA UAAGGGGUA@LB@3'-AUU...UUACU AAC CC UUG A AAGAA...CAA-5'@LB@ | |@LB@ 89 36@LB@@LB@@LB@Energy -13.21 kcal/mol@LB@Hybridization Energy -36.6547 kcal/mol@LB@Unfolding Energy - mRNA 11.779 kcal/mol@LB@Unfolding Energy - sRNA 14.1528 kcal/mol@LB@@LB@Position - mRNA 6 -- 79@LB@Position - sRNA 37 -- 88@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 151 |
0.00683 |
0.1741 |
bsu18660 |
yoaM |
-13.14 |
0.080203 |
141 -- 183 |
39 -- 78 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu18880 bsu20490 |
_____________140________________________________184\n_____________|__________________________________|\n5'-UUU...UAAAU_______________CAAA__A____AAAA____UAAAA...AUA-3'\n______________GAACGAUCGU_CCCA____CU_CUUA____CCCC\n______________:|||:|||||_||||____||_||||____||||\n______________UUUGUUAGCA_GGGU____GA_GAAU____GGGG\n3'-AUU...AACCG__________U____A__________AA______UAAAG...CAA-5'\n_____________|__________________________________|\n_____________79_________________________________38\n |
154 -- 160 |
57 -- 63 |
-25.2857 kcal/mol |
3.90608 kcal/mol |
11.0213 kcal/mol |
140 184@LB@ | |@LB@5'-UUU...UAAAU CAAA A AAAA UAAAA...AUA-3'@LB@ GAACGAUCGU CCCA CU CUUA CCCC@LB@ :|||:||||| |||| || |||| ||||@LB@ UUUGUUAGCA GGGU GA GAAU GGGG@LB@3'-AUU...AACCG U A AA UAAAG...CAA-5'@LB@ | |@LB@ 79 38@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -25.2857 kcal/mol@LB@Unfolding Energy - mRNA 3.90608 kcal/mol@LB@Unfolding Energy - sRNA 11.0213 kcal/mol@LB@@LB@Position - mRNA 141 -- 183@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 57 -- 63@LB@ |
| 184 |
0.008461 |
0.1751 |
bsu09120 |
dgcK |
-13.04 |
0.083992 |
38 -- 184 |
37 -- 168 |
diguanylate cyclase |
|
_____________37______________________________________185\n_____________|_______________________________________|\n5'-CAU...AAGUC___AA_______AAACUCUCCCUUCAA____________UACUA...GCA-3'\n______________UCC__AGCAGGU________________AUUUUCUCAUU\n______________|||__||||:||________________|:||||||||:\n______________AGG__UCGUUCA________________UGAAAGAGUAG\n3'-AUU...GUAAC___CG_______AGGUAUAUUACAACAG___________AAGUU...CAA-5'\n_____________|_______________________________________|\n_____________169_____________________________________36\n |
55 -- 61 |
102 -- 108 |
-22.9941 kcal/mol |
2.75938 kcal/mol |
10.7301 kcal/mol |
37 185@LB@ | |@LB@5'-CAU...AAGUC AA AAACUCUCCCUUCAA UACUA...GCA-3'@LB@ UCC AGCAGGU AUUUUCUCAUU@LB@ ||| ||||:|| |:||||||||:@LB@ AGG UCGUUCA UGAAAGAGUAG@LB@3'-AUU...GUAAC CG AGGUAUAUUACAACAG AAGUU...CAA-5'@LB@ | |@LB@ 169 36@LB@@LB@@LB@Energy -13.04 kcal/mol@LB@Hybridization Energy -22.9941 kcal/mol@LB@Unfolding Energy - mRNA 2.75938 kcal/mol@LB@Unfolding Energy - sRNA 10.7301 kcal/mol@LB@@LB@Position - mRNA 38 -- 184@LB@Position - sRNA 37 -- 168@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 34 |
0.001006 |
0.1147 |
bsu37620 |
rsfA |
-13.01 |
0.085239 |
64 -- 106 |
124 -- 165 |
prespore-specific transcriptional regulator RsfA |
|
_____________63_________________________________________________107\n_____________|__________________________________________________|\n5'-UUC...ACAUC__A_______UU__A_____UC_____________U_____CCGC_____CCUUC...ACG-3'\n______________AC_CUUCAUU__AC_AGGAU___GAC__UGUUC_C_GCAAG____UCCAU\n______________||_||||||:__||_||||:___|||__|||:|_|_|||||____|||||\n______________UG_GAAGUAG__UG_UCCUG___CUG__ACAGG_G_CGUUC____AGGUA\n3'-AUU...AAAAA__A_______U_________UAA___UA_____C_U_____A________UAUUA...CAA-5'\n_____________|__________________________________________________|\n_____________166________________________________________________123\n |
64 -- 70 |
159 -- 165 |
-31.4528 kcal/mol |
7.34844 kcal/mol |
10.3728 kcal/mol |
63 107@LB@ | |@LB@5'-UUC...ACAUC A UU A UC U CCGC CCUUC...ACG-3'@LB@ AC CUUCAUU AC AGGAU GAC UGUUC C GCAAG UCCAU@LB@ || ||||||: || ||||: ||| |||:| | ||||| |||||@LB@ UG GAAGUAG UG UCCUG CUG ACAGG G CGUUC AGGUA@LB@3'-AUU...AAAAA A U UAA UA C U A UAUUA...CAA-5'@LB@ | |@LB@ 166 123@LB@@LB@@LB@Energy -13.01 kcal/mol@LB@Hybridization Energy -31.4528 kcal/mol@LB@Unfolding Energy - mRNA 7.34844 kcal/mol@LB@Unfolding Energy - sRNA 10.3728 kcal/mol@LB@@LB@Position - mRNA 64 -- 106@LB@Position - sRNA 124 -- 165@LB@Position Seed - mRNA 64 -- 70@LB@Position Seed - sRNA 159 -- 165@LB@ |
| 67 |
0.002241 |
0.1242 |
bsu04040 |
ycsE |
-12.95 |
0.087665 |
83 -- 153 |
117 -- 184 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pe: enzyme' |
|
_____________82________________________________________________________________________________154\n_____________|_________________________________________________________________________________|\n________5'-AUC____C____AAG________C__GG___UUU__U___G_________GC______________GUCAU______UC_____CACCG...GCA-3'\n______________GGAU_GGUU___UCGUUGUG_UC__GGU___CA_UUG__UUUGUUUU__UGUUUUU__UUUCU_____GUUUGA__AC_CU\n______________|:||_||:|___||||||::_||__||:___||_|:|__::::|:|:__|:||:::__|||||_____:|||||__||_||\n______________CUUA_CCGA___AGCAACGU_AG__CCG___GU_AGC__GGGUAGAG__AUAAGGG__AAAGA_____UAAACU__UG_GA\n3'-AUU...CAUUA____________________U__AA___UUU__U___AU________A________GU_____AACUU______UU__C__AUUAA...CAA-5'\n_____________|_________________________________________________________________________________|\n_____________185_______________________________________________________________________________116\n |
52 -- 58 |
40 -- 46 |
-38.9686 kcal/mol |
11.5965 kcal/mol |
18.2899 kcal/mol |
82 154@LB@ | |@LB@ 5'-AUC C AAG C GG UUU U G GC GUCAU UC CACCG...GCA-3'@LB@ GGAU GGUU UCGUUGUG UC GGU CA UUG UUUGUUUU UGUUUUU UUUCU GUUUGA AC CU@LB@ |:|| ||:| ||||||:: || ||: || |:| ::::|:|: |:||::: ||||| :||||| || ||@LB@ CUUA CCGA AGCAACGU AG CCG GU AGC GGGUAGAG AUAAGGG AAAGA UAAACU UG GA@LB@3'-AUU...CAUUA U AA UUU U AU A GU AACUU UU C AUUAA...CAA-5'@LB@ | |@LB@ 185 116@LB@@LB@@LB@Energy -12.95 kcal/mol@LB@Hybridization Energy -38.9686 kcal/mol@LB@Unfolding Energy - mRNA 11.5965 kcal/mol@LB@Unfolding Energy - sRNA 18.2899 kcal/mol@LB@@LB@Position - mRNA 83 -- 153@LB@Position - sRNA 117 -- 184@LB@Position Seed - mRNA 52 -- 58@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 55 |
0.001813 |
0.1212 |
bsu30290 |
amyC |
-12.90 |
0.089506 |
91 -- 153 |
102 -- 159 |
ABC transporter permease |
|
_____________90_________________________________________________________________154\n_____________|__________________________________________________________________|\n5'-CUG...UCAAA_________GCC___UA________AC_____G_______UU___GCAU__________GA_____AGCUU...CUU-3'\n______________CGGCAGGAU___GGC__UG_CCGCA__GCAAG___CAUAU__AUG____UUGUCAUUUU__UCAUC\n______________|::|||||:___|:|__||_|||||__|||||___|||||__|||____||||||:|||__|||||\n______________GUUGUCCUG___CUG__AC_GGCGU__CGUUC___GUAUA__UAC____AACAGUGAAA__AGUAG\n3'-AUU...AAGUA_________UAA___UA__A____________AAG_____U__________________G______AAGUU...CAA-5'\n_____________|__________________________________________________________________|\n_____________160________________________________________________________________101\n |
139 -- 145 |
109 -- 115 |
-48.9264 kcal/mol |
23.4207 kcal/mol |
14.7573 kcal/mol |
90 154@LB@ | |@LB@5'-CUG...UCAAA GCC UA AC G UU GCAU GA AGCUU...CUU-3'@LB@ CGGCAGGAU GGC UG CCGCA GCAAG CAUAU AUG UUGUCAUUUU UCAUC@LB@ |::|||||: |:| || ||||| ||||| ||||| ||| ||||||:||| |||||@LB@ GUUGUCCUG CUG AC GGCGU CGUUC GUAUA UAC AACAGUGAAA AGUAG@LB@3'-AUU...AAGUA UAA UA A AAG U G AAGUU...CAA-5'@LB@ | |@LB@ 160 101@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -48.9264 kcal/mol@LB@Unfolding Energy - mRNA 23.4207 kcal/mol@LB@Unfolding Energy - sRNA 14.7573 kcal/mol@LB@@LB@Position - mRNA 91 -- 153@LB@Position - sRNA 102 -- 159@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 109 -- 115@LB@ |
| 154 |
0.00707 |
0.1751 |
bsu07830 |
yfkO |
-12.90 |
0.089530 |
138 -- 152 |
158 -- 172 |
NAD(P)H nitroreductase YfkO |
|
_____________137____________153\n_____________|______________|\n5'-GGA...GUACU______________UAAUG...AAU-3'\n______________UUUACUUUUUAUCA\n______________||||||:||:||||\n______________AAAUGAGAAGUAGU\n3'-AUU...AAAAA______________UGUCC...CAA-5'\n_____________|______________|\n_____________173____________157\n |
146 -- 152 |
158 -- 164 |
-13.59 kcal/mol |
1.38023 kcal/mol |
1.02713 kcal/mol |
137 153@LB@ | |@LB@5'-GGA...GUACU UAAUG...AAU-3'@LB@ UUUACUUUUUAUCA@LB@ ||||||:||:||||@LB@ AAAUGAGAAGUAGU@LB@3'-AUU...AAAAA UGUCC...CAA-5'@LB@ | |@LB@ 173 157@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -13.59 kcal/mol@LB@Unfolding Energy - mRNA 1.38023 kcal/mol@LB@Unfolding Energy - sRNA 1.02713 kcal/mol@LB@@LB@Position - mRNA 138 -- 152@LB@Position - sRNA 158 -- 172@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 96 |
0.003858 |
0.1541 |
bsu03700 |
gerKA |
-12.88 |
0.090279 |
45 -- 139 |
38 -- 125 |
spore germination protein KA |
|
_____________44_________________________________________140\n_____________|__________________________________________|\n5'-UUG...CCUCC_____UAG___CU___U________GA____A____G_____AACCC...CGG-3'\n______________GCUUU___UGU__AUC_UUGGCGGA__AGUU_UACC_CAUCU\n______________||||:___||:__|||_|||||::|__|:|:_||||_|||||\n______________CGAAG___ACG__UAG_AACCGUUU__UUAG_AUGG_GUAGA\n3'-AUU...CUUAC_____CA____U_____________G_____C__________GAAUA...CAA-5'\n_____________|__________________________________________|\n_____________126________________________________________37\n |
108 -- 114 |
65 -- 71 |
-29.7431 kcal/mol |
10.7852 kcal/mol |
9.08698 kcal/mol |
44 140@LB@ | |@LB@5'-UUG...CCUCC UAG CU U GA A G AACCC...CGG-3'@LB@ GCUUU UGU AUC UUGGCGGA AGUU UACC CAUCU@LB@ ||||: ||: ||| |||||::| |:|: |||| |||||@LB@ CGAAG ACG UAG AACCGUUU UUAG AUGG GUAGA@LB@3'-AUU...CUUAC CA U G C GAAUA...CAA-5'@LB@ | |@LB@ 126 37@LB@@LB@@LB@Energy -12.88 kcal/mol@LB@Hybridization Energy -29.7431 kcal/mol@LB@Unfolding Energy - mRNA 10.7852 kcal/mol@LB@Unfolding Energy - sRNA 9.08698 kcal/mol@LB@@LB@Position - mRNA 45 -- 139@LB@Position - sRNA 38 -- 125@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 65 -- 71@LB@ |
| 47 |
0.001488 |
0.1206 |
bsu18480 |
proH |
-12.75 |
0.095888 |
85 -- 112 |
155 -- 184 |
pyrroline-5-carboxylate reductase 1 |
bsu23800 |
_____________84_______________113\n_____________|________________|\n5'-AGC...UUUUC____________U___UAUUU...CAG-3'\n______________UGCUUUUCAUUA_ACA\n______________|:||:|||||:|_|||\n______________AUGAGAAGUAGU_UGU\n3'-AUU...AAAAA________________CCUGU...CAA-5'\n_____________|________________|\n_____________185______________154\n |
102 -- 108 |
158 -- 164 |
-13.4777 kcal/mol |
3.19807 kcal/mol |
1.41616 kcal/mol |
84 113@LB@ | |@LB@5'-AGC...UUUUC U UAUUU...CAG-3'@LB@ UGCUUUUCAUUA ACA@LB@ |:||:|||||:| |||@LB@ AUGAGAAGUAGU UGU@LB@3'-AUU...AAAAA CCUGU...CAA-5'@LB@ | |@LB@ 185 154@LB@@LB@@LB@Energy -12.75 kcal/mol@LB@Hybridization Energy -13.4777 kcal/mol@LB@Unfolding Energy - mRNA 3.19807 kcal/mol@LB@Unfolding Energy - sRNA 1.41616 kcal/mol@LB@@LB@Position - mRNA 85 -- 112@LB@Position - sRNA 155 -- 184@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 142 |
0.006636 |
0.1741 |
bsu22510 |
ypjC |
-12.74 |
0.096342 |
122 -- 155 |
156 -- 190 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________121_____________________________________________________________________________156\n_____________|_______________________________________________________________________________|\n5'-UAA...UCAUC_____A___GUUAGCUAAUU___AU___U______AA_____A_____C_____________C____UU__________CACUA...UGG-3'\n______________CUCUU_CAU___________CAG__ACG_UUGACA__UGUUU__AGCA_GUU________UG_UGUU__CUUUUUCAUC\n______________|||||_|||___________|||__||:_||||||__|||::__||||_|||________||_|||:__||||:|||||\n______________GAGAA_GUA___________GUC__UGU_AACUGU__ACAGG__UCGU_CAA________AC_ACAG__GAAAGAGUAG\n3'-AUU...AAAAU_________GUU___________C___________A______CG____U___GGUAUAUU__A____U___________AAGUU...CAA-5'\n_____________|_______________________________________________________________________________|\n_____________191_____________________________________________________________________________155\n |
146 -- 152 |
102 -- 108 |
-35.2093 kcal/mol |
10.3676 kcal/mol |
14.8184 kcal/mol |
121 156@LB@ | |@LB@5'-UAA...UCAUC A GUUAGCUAAUU AU U AA A C C UU CACUA...UGG-3'@LB@ CUCUU CAU CAG ACG UUGACA UGUUU AGCA GUU UG UGUU CUUUUUCAUC@LB@ ||||| ||| ||| ||: |||||| |||:: |||| ||| || |||: ||||:|||||@LB@ GAGAA GUA GUC UGU AACUGU ACAGG UCGU CAA AC ACAG GAAAGAGUAG@LB@3'-AUU...AAAAU GUU C A CG U GGUAUAUU A U AAGUU...CAA-5'@LB@ | |@LB@ 191 155@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -35.2093 kcal/mol@LB@Unfolding Energy - mRNA 10.3676 kcal/mol@LB@Unfolding Energy - sRNA 14.8184 kcal/mol@LB@@LB@Position - mRNA 122 -- 155@LB@Position - sRNA 156 -- 190@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 102 -- 108@LB@ |
