| 19 |
0.0005622 |
0.1011 |
bsu33310 |
fhuB |
-20.25 |
0.002499 |
30 -- 116 |
99 -- 184 |
iron(3+)-hydroxamate import system permease protein FhuB |
bsu01620 |
_____________29__________________________________________________________________117\n_____________|___________________________________________________________________|\n5'-AGG...UGCAA___A__________AAAAA____________UCC_______UU_____U___G______A_______ACCUU...AAG-3'\n______________UCA_UAACAGGGCA___________CGCGGC___GAGUUUC__GUAUA_AUG__GUCAU__UCUCAU\n______________|||_:||||||:||___________|||:||___:||||:|__:||||_|||__||||:__||||||\n______________AGU_GUUGUCCUGU___________GCGUCG___UUCAAGG__UAUAU_UAC__CAGUG__AGAGUA\n3'-AUU...UGAGA___A__________AACUGUAACAG___________________________AA_____AA______GAAGU...CAA-5'\n_____________|___________________________________________________________________|\n_____________185_________________________________________________________________98\n |
80 -- 86 |
126 -- 132 |
-44.6592 kcal/mol |
13.3222 kcal/mol |
14.475 kcal/mol |
29 117@LB@ | |@LB@5'-AGG...UGCAA A AAAAA UCC UU U G A ACCUU...AAG-3'@LB@ UCA UAACAGGGCA CGCGGC GAGUUUC GUAUA AUG GUCAU UCUCAU@LB@ ||| :||||||:|| |||:|| :||||:| :|||| ||| ||||: ||||||@LB@ AGU GUUGUCCUGU GCGUCG UUCAAGG UAUAU UAC CAGUG AGAGUA@LB@3'-AUU...UGAGA A AACUGUAACAG AA AA GAAGU...CAA-5'@LB@ | |@LB@ 185 98@LB@@LB@@LB@Energy -20.25 kcal/mol@LB@Hybridization Energy -44.6592 kcal/mol@LB@Unfolding Energy - mRNA 13.3222 kcal/mol@LB@Unfolding Energy - sRNA 14.475 kcal/mol@LB@@LB@Position - mRNA 30 -- 116@LB@Position - sRNA 99 -- 184@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 126 -- 132@LB@ |
| 11 |
0.0003253 |
0.1011 |
bsu31319 |
yuzI |
-20.00 |
0.002830 |
16 -- 82 |
99 -- 165 |
Evidence 4: Homologs of previously reported genes of unknown function; PubMedId: 16704729; Product type f : factor |
|
_____________15______________________________________________________________________83\n_____________|_______________________________________________________________________|\n5'-UAA...CAGUC_____U___UUC__U_G___________G_U____U______UUUUCCUCACUC_________A__G____CAUCA...ACU-3'\n______________CUUCA_UCA___GG_C_AUUG_C__UGU_C_CAGC__GUUCC____________GUCACUUUC_UA_CUUC\n______________|||||_|||___||_|_||||_|__|||_|_||||__|||||____________|||||||||_:|_||||\n______________GAAGU_AGU___CC_G_UAAC_G__ACA_G_GUCG__CAAGG____________CAGUGAAAG_GU_GAAG\n3'-AUU...AAUGA_________UGU__U______U_UA___G_C____UU_____UAUAUUACAA___________A__A____UUUCA...CAA-5'\n_____________|_______________________________________________________________________|\n_____________166_____________________________________________________________________98\n |
68 -- 74 |
107 -- 113 |
-43.9371 kcal/mol |
8.77558 kcal/mol |
17.9339 kcal/mol |
15 83@LB@ | |@LB@5'-UAA...CAGUC U UUC U G G U U UUUUCCUCACUC A G CAUCA...ACU-3'@LB@ CUUCA UCA GG C AUUG C UGU C CAGC GUUCC GUCACUUUC UA CUUC@LB@ ||||| ||| || | |||| | ||| | |||| ||||| ||||||||| :| ||||@LB@ GAAGU AGU CC G UAAC G ACA G GUCG CAAGG CAGUGAAAG GU GAAG@LB@3'-AUU...AAUGA UGU U U UA G C UU UAUAUUACAA A A UUUCA...CAA-5'@LB@ | |@LB@ 166 98@LB@@LB@@LB@Energy -20.00 kcal/mol@LB@Hybridization Energy -43.9371 kcal/mol@LB@Unfolding Energy - mRNA 8.77558 kcal/mol@LB@Unfolding Energy - sRNA 17.9339 kcal/mol@LB@@LB@Position - mRNA 16 -- 82@LB@Position - sRNA 99 -- 165@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 73 |
0.002371 |
0.1242 |
bsu00930 |
cysE |
-20.00 |
0.002833 |
192 -- 291 |
102 -- 187 |
serine acetyltransferase |
|
_____________191__________________________________________________________________________292\n_____________|____________________________________________________________________________|\n5'-CCU...AAUGC____AGAA__________C___GUUCGAUCAAGAUC__________AAG_____UU__AA__G_____AA______CCGGU...UAC-3'\n______________UCAA____GACAUUGAUA_UGU______________CCGCAGCAAG___C_UAU__UG__GU_AUUUU__CUUAUU\n______________||||____||||||||:|_|||______________||||||||||___|_|||__||__||_|:|||__||:||:\n______________AGUU____CUGUAACUGU_ACA______________GGCGUCGUUC___G_AUA__AC__CA_UGAAA__GAGUAG\n3'-AUU...GAAGU____GUC___________A___________________________AAG_U___UU__AA__G_____________AAGUU...CAA-5'\n_____________|____________________________________________________________________________|\n_____________188__________________________________________________________________________101\n |
256 -- 262 |
130 -- 136 |
-42.0244 kcal/mol |
15.3231 kcal/mol |
14.7636 kcal/mol |
191 292@LB@ | |@LB@5'-CCU...AAUGC AGAA C GUUCGAUCAAGAUC AAG UU AA G AA CCGGU...UAC-3'@LB@ UCAA GACAUUGAUA UGU CCGCAGCAAG C UAU UG GU AUUUU CUUAUU@LB@ |||| ||||||||:| ||| |||||||||| | ||| || || |:||| ||:||:@LB@ AGUU CUGUAACUGU ACA GGCGUCGUUC G AUA AC CA UGAAA GAGUAG@LB@3'-AUU...GAAGU GUC A AAG U UU AA G AAGUU...CAA-5'@LB@ | |@LB@ 188 101@LB@@LB@@LB@Energy -20.00 kcal/mol@LB@Hybridization Energy -42.0244 kcal/mol@LB@Unfolding Energy - mRNA 15.3231 kcal/mol@LB@Unfolding Energy - sRNA 14.7636 kcal/mol@LB@@LB@Position - mRNA 192 -- 291@LB@Position - sRNA 102 -- 187@LB@Position Seed - mRNA 256 -- 262@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 8 |
0.0001871 |
0.08823 |
bsu22860 |
yphA |
-19.72 |
0.003251 |
3 -- 123 |
37 -- 178 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________2_______________________________________________________________________________________________________124\n_____________|_______________________________________________________________________________________________________|\n5'-UCG...AAGGC________CCG______________________AC_G__C_______GAUUC______________AAGCUUUGAGUUUUU________CUGUCUU_______GAUCA...UUA-3'\n______________UUGUCGCU___UCA_________UAGUGAAUGG__U_GU_GCGAUCU_____GAC__UC_UGCCUG_______________UCUCUUGU_______UUCCCAU\n______________|||||:||___|||_________||:|||||||__|_||_||:||||_____:||__||_|:||::_______________||||||:|_______|:|||||\n______________AACAGUGA___AGU_________AUUACUUACC__A_CA_CGUUAGA_____UUG__AG_AUGGGU_______________AGAGAAUA_______AGGGGUA\n3'-AUU...AUUAC________AAG___AGAAGUUUC__________GA_G__A_______ACCGU___UU__C___________________________________________AAGAA...CAA-5'\n_____________|_______________________________________________________________________________________________________|\n_____________179_____________________________________________________________________________________________________36\n |
117 -- 123 |
37 -- 43 |
-53.1639 kcal/mol |
19.1133 kcal/mol |
22.4377 kcal/mol |
2 124@LB@ | |@LB@5'-UCG...AAGGC CCG AC G C GAUUC AAGCUUUGAGUUUUU CUGUCUU GAUCA...UUA-3'@LB@ UUGUCGCU UCA UAGUGAAUGG U GU GCGAUCU GAC UC UGCCUG UCUCUUGU UUCCCAU@LB@ |||||:|| ||| ||:||||||| | || ||:|||| :|| || |:||:: ||||||:| |:|||||@LB@ AACAGUGA AGU AUUACUUACC A CA CGUUAGA UUG AG AUGGGU AGAGAAUA AGGGGUA@LB@3'-AUU...AUUAC AAG AGAAGUUUC GA G A ACCGU UU C AAGAA...CAA-5'@LB@ | |@LB@ 179 36@LB@@LB@@LB@Energy -19.72 kcal/mol@LB@Hybridization Energy -53.1639 kcal/mol@LB@Unfolding Energy - mRNA 19.1133 kcal/mol@LB@Unfolding Energy - sRNA 22.4377 kcal/mol@LB@@LB@Position - mRNA 3 -- 123@LB@Position - sRNA 37 -- 178@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 60 |
0.001877 |
0.1212 |
bsu12040 |
yjdG |
-19.63 |
0.003403 |
61 -- 106 |
39 -- 81 |
N-acetyltransferase YjdG |
|
_____________60_______________________________________________107\n_____________|________________________________________________|\n5'-ACC...CUACC____UUU_____G_______UC___________AC____UCAA_____CUCAA...UAG-3'\n______________CGUU___GGUCU_GGCAAAC__UC__ACCUGUC__UCUU____UCCCC\n______________||||___::|||_|||||||__||__|||::||__||||____|||||\n______________GCAA___UUAGA_CCGUUUG__AG__UGGGUAG__AGAA____AGGGG\n3'-AUU...CCGAA____CG______A_______UU__CA_____________UA_______UAAAG...CAA-5'\n_____________|________________________________________________|\n_____________82_______________________________________________38\n |
85 -- 91 |
50 -- 56 |
-36.328 kcal/mol |
6.99682 kcal/mol |
13.0865 kcal/mol |
60 107@LB@ | |@LB@5'-ACC...CUACC UUU G UC AC UCAA CUCAA...UAG-3'@LB@ CGUU GGUCU GGCAAAC UC ACCUGUC UCUU UCCCC@LB@ |||| ::||| ||||||| || |||::|| |||| |||||@LB@ GCAA UUAGA CCGUUUG AG UGGGUAG AGAA AGGGG@LB@3'-AUU...CCGAA CG A UU CA UA UAAAG...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -19.63 kcal/mol@LB@Hybridization Energy -36.328 kcal/mol@LB@Unfolding Energy - mRNA 6.99682 kcal/mol@LB@Unfolding Energy - sRNA 13.0865 kcal/mol@LB@@LB@Position - mRNA 61 -- 106@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 64 |
0.002007 |
0.1215 |
bsu37680 |
ywfH |
-19.51 |
0.003623 |
4 -- 58 |
32 -- 83 |
bacilysin biosynthesis oxidoreductase YwfH |
|
_____________3___________________________________________________________59\n_____________|___________________________________________________________|\n_________5'-AA___G______U____U__C_____________U__G____AUACAC_______A_____AUAUG...AUU-3'\n______________UUU_GUU_CA_UCUU_GU_GGCAAUC__ACCU_UC_CUUG______UUCCUCA_UUCUU\n______________:||_|||_||_||||_|:_::|||||__|||:_||_|||:______||||:||_|||||\n______________GAA_CAA_GU_AGAA_CG_UUGUUAG__UGGG_AG_GAAU______AAGGGGU_AAGAA\n3'-AUU...UUACC___G___C__U____C__U_______CA____U__A_________________A_____ACUUU...CAA-5'\n_____________|___________________________________________________________|\n_____________84__________________________________________________________31\n |
46 -- 52 |
38 -- 44 |
-36.3278 kcal/mol |
5.00359 kcal/mol |
14.596 kcal/mol |
3 59@LB@ | |@LB@ 5'-AA G U U C U G AUACAC A AUAUG...AUU-3'@LB@ UUU GUU CA UCUU GU GGCAAUC ACCU UC CUUG UUCCUCA UUCUU@LB@ :|| ||| || |||| |: ::||||| |||: || |||: ||||:|| |||||@LB@ GAA CAA GU AGAA CG UUGUUAG UGGG AG GAAU AAGGGGU AAGAA@LB@3'-AUU...UUACC G C U C U CA U A A ACUUU...CAA-5'@LB@ | |@LB@ 84 31@LB@@LB@@LB@Energy -19.51 kcal/mol@LB@Hybridization Energy -36.3278 kcal/mol@LB@Unfolding Energy - mRNA 5.00359 kcal/mol@LB@Unfolding Energy - sRNA 14.596 kcal/mol@LB@@LB@Position - mRNA 4 -- 58@LB@Position - sRNA 32 -- 83@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 144 |
0.006712 |
0.1741 |
bsu25040 |
yqgB |
-19.28 |
0.004052 |
60 -- 160 |
55 -- 168 |
Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 15294815; Product type ph: phenotype |
|
_____________59_____________________________________________________161\n_____________|______________________________________________________|\n5'-UAA...AAAAC__C_______AACA__CAAU______A_______AG___CCAUCC_________ACUCU...UUU-3'\n______________CG_UGCAAUC____GC____ACAA_C_UAUCCAU__CUU______UCCCAUUUU\n______________||_|||||||____||____||||_|_||:||||__|||______:|||||||:\n______________GC_ACGUUAG____CG____UGUU_G_AUGGGUA__GAA______GGGGUAAAG\n3'-AUU...CCGAA__A_______AAC___UU______A_C_______GA___UAA____________AAACU...CAA-5'\n_____________|______________________________________________________|\n_____________169____________________________________________________54\n |
105 -- 111 |
34 -- 40 |
-33.5165 kcal/mol |
5.00067 kcal/mol |
13.93 kcal/mol |
59 161@LB@ | |@LB@5'-UAA...AAAAC C AACA CAAU A AG CCAUCC ACUCU...UUU-3'@LB@ CG UGCAAUC GC ACAA C UAUCCAU CUU UCCCAUUUU@LB@ || ||||||| || |||| | ||:|||| ||| :|||||||:@LB@ GC ACGUUAG CG UGUU G AUGGGUA GAA GGGGUAAAG@LB@3'-AUU...CCGAA A AAC UU A C GA UAA AAACU...CAA-5'@LB@ | |@LB@ 169 54@LB@@LB@@LB@Energy -19.28 kcal/mol@LB@Hybridization Energy -33.5165 kcal/mol@LB@Unfolding Energy - mRNA 5.00067 kcal/mol@LB@Unfolding Energy - sRNA 13.93 kcal/mol@LB@@LB@Position - mRNA 60 -- 160@LB@Position - sRNA 55 -- 168@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 143 |
0.006663 |
0.1741 |
bsu05790 |
ydhK |
-18.88 |
0.004963 |
179 -- 222 |
125 -- 163 |
Evidence 5: No homology to any previously reported sequences |
|
_____________178__________________________________________223\n_____________|____________________________________________|\n5'-GUG...UAGUA_______G_______GUUUU____________G__AA___UA__GGAAA...UUU-3'\n______________UUAUCGA_AGGGCAU_____CAUUGUCUGCGG_CA__AGU__CA\n______________|:|||:|_|||:|||_____|||||||:||:|_||__|||__||\n______________AGUAGUU_UCCUGUA_____GUAACAGGCGUC_GU__UCA__GU\n3'-AUU...UGAGA_______G_______ACU______________________AG__AUAUU...CAA-5'\n_____________|____________________________________________|\n_____________164__________________________________________124\n |
204 -- 210 |
134 -- 140 |
-40.9125 kcal/mol |
11.6904 kcal/mol |
10.3584 kcal/mol |
178 223@LB@ | |@LB@5'-GUG...UAGUA G GUUUU G AA UA GGAAA...UUU-3'@LB@ UUAUCGA AGGGCAU CAUUGUCUGCGG CA AGU CA@LB@ |:|||:| |||:||| |||||||:||:| || ||| ||@LB@ AGUAGUU UCCUGUA GUAACAGGCGUC GU UCA GU@LB@3'-AUU...UGAGA G ACU AG AUAUU...CAA-5'@LB@ | |@LB@ 164 124@LB@@LB@@LB@Energy -18.88 kcal/mol@LB@Hybridization Energy -40.9125 kcal/mol@LB@Unfolding Energy - mRNA 11.6904 kcal/mol@LB@Unfolding Energy - sRNA 10.3584 kcal/mol@LB@@LB@Position - mRNA 179 -- 222@LB@Position - sRNA 125 -- 163@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 134 -- 140@LB@ |
| 112 |
0.00483 |
0.1671 |
bsu26010 |
yqbQ |
-18.75 |
0.005285 |
180 -- 239 |
82 -- 139 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu12700 |
_____________179______________________________________________________________240\n_____________|________________________________________________________________|\n5'-CUA...AAAUA_______________G______ACA__AUAGA___________A______AAACG_________GAGCU...UUA-3'\n______________CCGCAGUAAG___CA_GUGAUG___UG_____AC_UUUUCGUC_UUAAAG_____GAAUGGCUU\n______________||||||:|||___||_:|:|||___||_____||_||:||:||_|:||||_____|||||||||\n______________GGCGUCGUUC___GU_UAUUAC___AC_____UG_AAGAGUAG_AGUUUC_____CUUACCGAA\n3'-AUU...UAACA__________AAG__A______A____AG_____A________A______AUUA__________GCAAC...CAA-5'\n_____________|________________________________________________________________|\n_____________140______________________________________________________________81\n |
233 -- 239 |
82 -- 88 |
-46.2512 kcal/mol |
11.9879 kcal/mol |
18.3017 kcal/mol |
179 240@LB@ | |@LB@5'-CUA...AAAUA G ACA AUAGA A AAACG GAGCU...UUA-3'@LB@ CCGCAGUAAG CA GUGAUG UG AC UUUUCGUC UUAAAG GAAUGGCUU@LB@ ||||||:||| || :|:||| || || ||:||:|| |:|||| |||||||||@LB@ GGCGUCGUUC GU UAUUAC AC UG AAGAGUAG AGUUUC CUUACCGAA@LB@3'-AUU...UAACA AAG A A AG A A AUUA GCAAC...CAA-5'@LB@ | |@LB@ 140 81@LB@@LB@@LB@Energy -18.75 kcal/mol@LB@Hybridization Energy -46.2512 kcal/mol@LB@Unfolding Energy - mRNA 11.9879 kcal/mol@LB@Unfolding Energy - sRNA 18.3017 kcal/mol@LB@@LB@Position - mRNA 180 -- 239@LB@Position - sRNA 82 -- 139@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 82 -- 88@LB@ |
| 103 |
0.004238 |
0.1594 |
bsu09690 |
nhaX |
-18.33 |
0.006526 |
38 -- 80 |
34 -- 77 |
stress response protein NhaX |
|
_____________37________________________________________________81\n_____________|_________________________________________________|\n5'-GGU...AACAC____AUUC_______U____________________CCA____G_____AUCUU...CCG-3'\n______________GCAA____CUUGGCA_AGCAA____ACCC_UC_CUU___UUCU_AUUUC\n______________||||____|||||||_|:|||____||||_||_|||___|:|:_|||||\n______________CGUU____GAACCGU_UUGUU____UGGG_AG_GAA___AGGG_UAAAG\n3'-AUU...AGCAA____A________________AGCA____U__A___UA_____G_____AAACU...CAA-5'\n_____________|_________________________________________________|\n_____________78________________________________________________33\n |
46 -- 52 |
66 -- 72 |
-32.0538 kcal/mol |
3.14542 kcal/mol |
13.7939 kcal/mol |
37 81@LB@ | |@LB@5'-GGU...AACAC AUUC U CCA G AUCUU...CCG-3'@LB@ GCAA CUUGGCA AGCAA ACCC UC CUU UUCU AUUUC@LB@ |||| ||||||| |:||| |||| || ||| |:|: |||||@LB@ CGUU GAACCGU UUGUU UGGG AG GAA AGGG UAAAG@LB@3'-AUU...AGCAA A AGCA U A UA G AAACU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -18.33 kcal/mol@LB@Hybridization Energy -32.0538 kcal/mol@LB@Unfolding Energy - mRNA 3.14542 kcal/mol@LB@Unfolding Energy - sRNA 13.7939 kcal/mol@LB@@LB@Position - mRNA 38 -- 80@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 66 -- 72@LB@ |
