| 145 |
0.006739 |
0.1741 |
bsu12460 |
xlyB |
-11.80 |
0.146979 |
21 -- 64 |
155 -- 186 |
N-acetylmuramoyl-L-alanine amidase XlyB |
bsu12810 bsu25710 bsu25900 bsu21410 |
_____________20____________________________________65\n_____________|_____________________________________|\n5'-UCC...CCACA_____C_G_____AUUUCUC____CU_______A___UUAAA...CGG-3'\n______________UGCUC_U_AUUAA_______CGUU__CAUUGUC_GCA\n______________|:|||_|_||:||_______|:||__|||||||_|||\n______________AUGAG_A_UAGUU_______GUAA__GUAACAG_CGU\n3'-AUU...AAAAA_____A_G_____GUCCU______CU_______G___CGUUC...CAA-5'\n_____________|_____________________________________|\n_____________187___________________________________154\n |
54 -- 60 |
139 -- 145 |
-22.5299 kcal/mol |
4.58402 kcal/mol |
8.54622 kcal/mol |
20 65@LB@ | |@LB@5'-UCC...CCACA C G AUUUCUC CU A UUAAA...CGG-3'@LB@ UGCUC U AUUAA CGUU CAUUGUC GCA@LB@ |:||| | ||:|| |:|| ||||||| |||@LB@ AUGAG A UAGUU GUAA GUAACAG CGU@LB@3'-AUU...AAAAA A G GUCCU CU G CGUUC...CAA-5'@LB@ | |@LB@ 187 154@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -22.5299 kcal/mol@LB@Unfolding Energy - mRNA 4.58402 kcal/mol@LB@Unfolding Energy - sRNA 8.54622 kcal/mol@LB@@LB@Position - mRNA 21 -- 64@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 139 -- 145@LB@ |
| 41 |
0.00131 |
0.1206 |
bsu01580 |
ybaR |
-15.69 |
0.023818 |
44 -- 97 |
34 -- 84 |
sulfate transporter YbaR |
|
_____________43________________________________________________________________98\n_____________|_________________________________________________________________|\n5'-CAG...UUAUU____UACU______A__A___CUC____CCU___U_____A_______A_GU_____________UCUCC...CGA-3'\n______________AUGA____CUUUGU_UG_CAA___CUUG___CAA_ACAAU__UACUCA_C_____UUCCCCGUUU\n______________||||____|||:||_||_|||___||||___|||_|||||__|||:||_|_____||||||:|||\n______________UACU____GAAGCA_AC_GUU___GAAC___GUU_UGUUA__AUGGGU_G_____AAGGGGUAAA\n3'-AUU...UUCAU____UACC_____________A______C___________GC______A_AGAAU__________GAAAC...CAA-5'\n_____________|_________________________________________________________________|\n_____________85________________________________________________________________33\n |
90 -- 96 |
35 -- 41 |
-34.7178 kcal/mol |
7.59764 kcal/mol |
14.4179 kcal/mol |
43 98@LB@ | |@LB@5'-CAG...UUAUU UACU A A CUC CCU U A A GU UCUCC...CGA-3'@LB@ AUGA CUUUGU UG CAA CUUG CAA ACAAU UACUCA C UUCCCCGUUU@LB@ |||| |||:|| || ||| |||| ||| ||||| |||:|| | ||||||:|||@LB@ UACU GAAGCA AC GUU GAAC GUU UGUUA AUGGGU G AAGGGGUAAA@LB@3'-AUU...UUCAU UACC A C GC A AGAAU GAAAC...CAA-5'@LB@ | |@LB@ 85 33@LB@@LB@@LB@Energy -15.69 kcal/mol@LB@Hybridization Energy -34.7178 kcal/mol@LB@Unfolding Energy - mRNA 7.59764 kcal/mol@LB@Unfolding Energy - sRNA 14.4179 kcal/mol@LB@@LB@Position - mRNA 44 -- 97@LB@Position - sRNA 34 -- 84@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 75 |
0.002405 |
0.1242 |
bsu01600 |
ybbA |
-13.26 |
0.075734 |
128 -- 177 |
34 -- 77 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 12354229 2974033; Product type pe: enzyme' |
|
_____________127______________________________________________178\n_____________|________________________________________________|\n5'-UCA...CGCAG___UC_________________________GGA_______________AUAUC...UUU-3'\n______________GCA__AUUUUGGCG_________AUCC_UC____GUUCCUUAUUUCUU\n______________|||__||:|||||:_________|:||_||____:||||::|||||||\n______________CGU__UAGAACCGU_________UGGG_AG____UAAGGGGUAAAGAA\n3'-AUU...AGCAA______________UUGUUAGCA____U__AGAA______________ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________78_______________________________________________33\n |
158 -- 164 |
32 -- 38 |
-31.1473 kcal/mol |
6.80918 kcal/mol |
13.8943 kcal/mol |
127 178@LB@ | |@LB@5'-UCA...CGCAG UC GGA AUAUC...UUU-3'@LB@ GCA AUUUUGGCG AUCC UC GUUCCUUAUUUCUU@LB@ ||| ||:|||||: |:|| || :||||::|||||||@LB@ CGU UAGAACCGU UGGG AG UAAGGGGUAAAGAA@LB@3'-AUU...AGCAA UUGUUAGCA U AGAA ACUUU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -13.26 kcal/mol@LB@Hybridization Energy -31.1473 kcal/mol@LB@Unfolding Energy - mRNA 6.80918 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 128 -- 177@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 185 |
0.008526 |
0.1751 |
bsu02370 |
ybgA |
-10.25 |
0.283898 |
252 -- 276 |
59 -- 82 |
GntR family transcriptional regulator |
|
_____________251_________________________277\n_____________|___________________________|\n5'-GAU...UUAAA___G____AAU___A____________AGCUG...UUU-3'\n______________UCG__GCA___AUC__GGCGAAUGAUC\n______________|||__|||___|||__|||:||::|||\n______________AGC__CGU___UAG__CCGUUUGUUAG\n3'-AUU...ACCGA___AA_________AA___________CAUGG...CAA-5'\n_____________|___________________________|\n_____________83__________________________58\n |
266 -- 272 |
63 -- 69 |
-19.3785 kcal/mol |
3.33361 kcal/mol |
6.42004 kcal/mol |
251 277@LB@ | |@LB@5'-GAU...UUAAA G AAU A AGCUG...UUU-3'@LB@ UCG GCA AUC GGCGAAUGAUC@LB@ ||| ||| ||| |||:||::|||@LB@ AGC CGU UAG CCGUUUGUUAG@LB@3'-AUU...ACCGA AA AA CAUGG...CAA-5'@LB@ | |@LB@ 83 58@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -19.3785 kcal/mol@LB@Unfolding Energy - mRNA 3.33361 kcal/mol@LB@Unfolding Energy - sRNA 6.42004 kcal/mol@LB@@LB@Position - mRNA 252 -- 276@LB@Position - sRNA 59 -- 82@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 63 -- 69@LB@ |
| 164 |
0.007653 |
0.1751 |
bsu02110 |
ybyB |
-7.82 |
0.651200 |
271 -- 286 |
38 -- 56 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________270_____287\n_____________|_______|\n5'-UUG...AAAAG_______AAAUC...AAU-3'\n______________ACCCGUC\n______________||||:||\n______________UGGGUAG\n3'-AUU...UAGCA_______AGAAU...CAA-5'\n_____________|_______|\n_____________57______37\n |
271 -- 277 |
50 -- 56 |
-11.5721 kcal/mol |
0.816393 kcal/mol |
3.06627 kcal/mol |
270 287@LB@ | |@LB@5'-UUG...AAAAG AAAUC...AAU-3'@LB@ ACCCGUC@LB@ ||||:||@LB@ UGGGUAG@LB@3'-AUU...UAGCA AGAAU...CAA-5'@LB@ | |@LB@ 57 37@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -11.5721 kcal/mol@LB@Unfolding Energy - mRNA 0.816393 kcal/mol@LB@Unfolding Energy - sRNA 3.06627 kcal/mol@LB@@LB@Position - mRNA 271 -- 286@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 189 |
0.008686 |
0.1751 |
bsu03110 |
ycgH |
-10.16 |
0.294825 |
102 -- 123 |
120 -- 141 |
transporter |
|
_____________101____________________124\n_____________|______________________|\n5'-UUU...UGAGC______UUCCUUC_________AAAGA...UCG-3'\n______________GUCUGC_______UCCAUGUAA\n______________|||:||_______|||||:|||\n______________CAGGCG_______AGGUAUAUU\n3'-AUU...UGUAA______UCGUUCA_________ACAAC...CAA-5'\n_____________|______________________|\n_____________142____________________119\n |
117 -- 123 |
120 -- 126 |
-19.5867 kcal/mol |
5.23504 kcal/mol |
6.76593 kcal/mol |
101 124@LB@ | |@LB@5'-UUU...UGAGC UUCCUUC AAAGA...UCG-3'@LB@ GUCUGC UCCAUGUAA@LB@ |||:|| |||||:|||@LB@ CAGGCG AGGUAUAUU@LB@3'-AUU...UGUAA UCGUUCA ACAAC...CAA-5'@LB@ | |@LB@ 142 119@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -19.5867 kcal/mol@LB@Unfolding Energy - mRNA 5.23504 kcal/mol@LB@Unfolding Energy - sRNA 6.76593 kcal/mol@LB@@LB@Position - mRNA 102 -- 123@LB@Position - sRNA 120 -- 141@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 163 |
0.007546 |
0.1751 |
bsu03210 |
ycgN |
-11.47 |
0.170088 |
123 -- 268 |
12 -- 147 |
1-pyrroline-5-carboxylate dehydrogenase |
|
_____________122______________________________________________________269\n_____________|________________________________________________________|\n5'-CAA...CCUUA__________A___AU__A_AA______AAGAUCAAAACAACG_____________AAAAG...UAG-3'\n______________CAAACA__CG_GCC__UC_C__AUUUCC_______________UGGAAGCGUUUAA\n______________||||||__||_:||__||_|__|||:||_______________||:||:||:||||\n______________GUUUGU__GC_UGG__AG_G__UAAGGG_______________ACUUUUGCGAAUU\n3'-AUU...GAACC______UA__A___GU__A_AA______GUAAAGAAACUUUAA_____________AAAGA...CAA-5'\n_____________|________________________________________________________|\n_____________148______________________________________________________11\n |
262 -- 268 |
12 -- 18 |
-30.9991 kcal/mol |
6.38057 kcal/mol |
15.527 kcal/mol |
122 269@LB@ | |@LB@5'-CAA...CCUUA A AU A AA AAGAUCAAAACAACG AAAAG...UAG-3'@LB@ CAAACA CG GCC UC C AUUUCC UGGAAGCGUUUAA@LB@ |||||| || :|| || | |||:|| ||:||:||:||||@LB@ GUUUGU GC UGG AG G UAAGGG ACUUUUGCGAAUU@LB@3'-AUU...GAACC UA A GU A AA GUAAAGAAACUUUAA AAAGA...CAA-5'@LB@ | |@LB@ 148 11@LB@@LB@@LB@Energy -11.47 kcal/mol@LB@Hybridization Energy -30.9991 kcal/mol@LB@Unfolding Energy - mRNA 6.38057 kcal/mol@LB@Unfolding Energy - sRNA 15.527 kcal/mol@LB@@LB@Position - mRNA 123 -- 268@LB@Position - sRNA 12 -- 147@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 12 -- 18@LB@ |
| 81 |
0.002798 |
0.1325 |
bsu03250 |
ycgR |
-7.37 |
0.727097 |
84 -- 130 |
48 -- 86 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pt: transporter' |
|
_____________83_______________________________________________131\n_____________|________________________________________________|\n5'-GCG...UACUC__________G____CUAACCAAAGUG________CUUUC___UC___CGUCA...GAA-3'\n______________GGCUUU_UUG_AAUC____________AAACGAUC_____CCC__CUC\n______________|||||:_|||_||||____________||||:|||_____|||__|||\n______________CCGAAG_AAC_UUAG____________UUUGUUAG_____GGG__GAG\n3'-AUU...ACUUA______C___G____AACCG_______________CAU_____UA___AAUAA...CAA-5'\n_____________|________________________________________________|\n_____________87_______________________________________________47\n |
111 -- 117 |
59 -- 65 |
-26.5811 kcal/mol |
9.91993 kcal/mol |
9.18845 kcal/mol |
83 131@LB@ | |@LB@5'-GCG...UACUC G CUAACCAAAGUG CUUUC UC CGUCA...GAA-3'@LB@ GGCUUU UUG AAUC AAACGAUC CCC CUC@LB@ |||||: ||| |||| ||||:||| ||| |||@LB@ CCGAAG AAC UUAG UUUGUUAG GGG GAG@LB@3'-AUU...ACUUA C G AACCG CAU UA AAUAA...CAA-5'@LB@ | |@LB@ 87 47@LB@@LB@@LB@Energy -7.37 kcal/mol@LB@Hybridization Energy -26.5811 kcal/mol@LB@Unfolding Energy - mRNA 9.91993 kcal/mol@LB@Unfolding Energy - sRNA 9.18845 kcal/mol@LB@@LB@Position - mRNA 84 -- 130@LB@Position - sRNA 48 -- 86@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 134 |
0.00626 |
0.1741 |
bsu03360 |
yciC |
-8.59 |
0.518951 |
99 -- 108 |
130 -- 139 |
metal chaperone YciC |
|
_____________98_______109\n_____________|________|\n5'-AGG...GGGCG________AAAUG...UAG-3'\n______________GCAGCGAG\n______________|||||:||\n______________CGUCGUUC\n3'-AUU...ACAGG________AAGGU...CAA-5'\n_____________|________|\n_____________140______129\n |
102 -- 108 |
130 -- 136 |
-12.856 kcal/mol |
2.76877 kcal/mol |
2.9981 kcal/mol |
98 109@LB@ | |@LB@5'-AGG...GGGCG AAAUG...UAG-3'@LB@ GCAGCGAG@LB@ |||||:||@LB@ CGUCGUUC@LB@3'-AUU...ACAGG AAGGU...CAA-5'@LB@ | |@LB@ 140 129@LB@@LB@@LB@Energy -8.59 kcal/mol@LB@Hybridization Energy -12.856 kcal/mol@LB@Unfolding Energy - mRNA 2.76877 kcal/mol@LB@Unfolding Energy - sRNA 2.9981 kcal/mol@LB@@LB@Position - mRNA 99 -- 108@LB@Position - sRNA 130 -- 139@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 24 |
0.0006259 |
0.1011 |
bsu03380 |
yckB |
-16.82 |
0.013754 |
20 -- 129 |
16 -- 148 |
ABC transporter substrate-binding protein |
|
_____________19_________________________________________________________________________________________________________________________________________________130\n_____________|__________________________________________________________________________________________________________________________________________________|\n5'-UGC...UCCUU__U____C_______CA___U___A________UUUUUUCA_______C_____CC_____U__AC_______________________________CCC________G_____UGUAUA_A__A___GAACUU____________GAUCA...CCA-3'\n______________GG_ACGU_UGGCAUU__UCC_UGG_CAA_UUCC___________UCAC__UCUU__CUUCA_AG_________GCUUU_____________UAAACA_____ACCCAU_CUUUU______C_CA_UUC_______UUUGAAAACGC\n______________||_||:|_||:||||__|||_::|_|||_||||___________||||__|||:__|||||_||_________||||:_____________:|||||_____||||||_||:||______|_||_|||_______|||||||||||\n______________CC_UGUA_ACUGUAA__AGG_GUC_GUU_AAGG___________AGUG__AGAG__GAAGU_UC_________CGAAG_____________GUUUGU_____UGGGUA_GAGAA______G_GU_AAG_______AAACUUUUGCG\n3'-AUU...GUUGU_______________C____C_______C____UAUAUUACAAC____AA____UA_____U__AUUACUUAC_____CAACGUUAGAACC______UAGCA____________UAAGG_____A___AAACUUU___________AAUUA...CAA-5'\n_____________|__________________________________________________________________________________________________________________________________________________|\n_____________149________________________________________________________________________________________________________________________________________________15\n |
123 -- 129 |
16 -- 22 |
-61.2162 kcal/mol |
13.3554 kcal/mol |
33.3199 kcal/mol |
19 130@LB@ | |@LB@5'-UGC...UCCUU U C CA U A UUUUUUCA C CC U AC CCC G UGUAUA A A GAACUU GAUCA...CCA-3'@LB@ GG ACGU UGGCAUU UCC UGG CAA UUCC UCAC UCUU CUUCA AG GCUUU UAAACA ACCCAU CUUUU C CA UUC UUUGAAAACGC@LB@ || ||:| ||:|||| ||| ::| ||| |||| |||| |||: ||||| || ||||: :||||| |||||| ||:|| | || ||| |||||||||||@LB@ CC UGUA ACUGUAA AGG GUC GUU AAGG AGUG AGAG GAAGU UC CGAAG GUUUGU UGGGUA GAGAA G GU AAG AAACUUUUGCG@LB@3'-AUU...GUUGU C C C UAUAUUACAAC AA UA U AUUACUUAC CAACGUUAGAACC UAGCA UAAGG A AAACUUU AAUUA...CAA-5'@LB@ | |@LB@ 149 15@LB@@LB@@LB@Energy -16.82 kcal/mol@LB@Hybridization Energy -61.2162 kcal/mol@LB@Unfolding Energy - mRNA 13.3554 kcal/mol@LB@Unfolding Energy - sRNA 33.3199 kcal/mol@LB@@LB@Position - mRNA 20 -- 129@LB@Position - sRNA 16 -- 148@LB@Position Seed - mRNA 123 -- 129@LB@Position Seed - sRNA 16 -- 22@LB@ |
