| 51 |
0.001644 |
0.1212 |
bsu29870 |
ytzB |
-11.72 |
0.152375 |
23 -- 59 |
33 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________22__________________________________________60\n_____________|___________________________________________|\n5'-CUG...AUUGC___________________________________________AAAAG...AAG-3'\n______________UUAACAGGAU_UUGGC__UGUU__________UCUGUAUAAUG\n______________|:|||||||:_|||:|__|||:__________||::|||||||\n______________AGUUGUCCUG_AACUG__ACAG__________AGGUAUAUUAC\n3'-AUU...GAAGU__________U_____UA____GCGUCGUUCA___________AACAG...CAA-5'\n_____________|___________________________________________|\n_____________86__________________________________________32\n |
163 -- 169 |
118 -- 124 |
-27.3559 kcal/mol |
7.06665 kcal/mol |
10.6143 kcal/mol |
22 60@LB@ | |@LB@5'-CUG...AUUGC AAAAG...AAG-3'@LB@ UUAACAGGAU UUGGC UGUU UCUGUAUAAUG@LB@ |:|||||||: |||:| |||: ||::|||||||@LB@ AGUUGUCCUG AACUG ACAG AGGUAUAUUAC@LB@3'-AUU...GAAGU U UA GCGUCGUUCA AACAG...CAA-5'@LB@ | |@LB@ 86 32@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -27.3559 kcal/mol@LB@Unfolding Energy - mRNA 7.06665 kcal/mol@LB@Unfolding Energy - sRNA 10.6143 kcal/mol@LB@@LB@Position - mRNA 23 -- 59@LB@Position - sRNA 33 -- 85@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 37 |
0.00122 |
0.1206 |
bsu31150 |
yubB |
-14.80 |
0.036692 |
271 -- 292 |
145 -- 165 |
undecaprenyl-diphosphatase |
|
_____________270____________________293\n_____________|______________________|\n5'-UAU...GCCGG____U____________A____CAUAGUAG-3'\n______________UUUC_UCGACAGGACAU_UGAU\n______________:|||_||:|||||||||_|||:\n______________GAAG_AGUUGUCCUGUA_ACUG\n3'-AUU...AAUGA____U_________________UAACA...CAA-5'\n_____________|______________________|\n_____________166____________________144\n |
281 -- 287 |
149 -- 155 |
-24.6442 kcal/mol |
4.4819 kcal/mol |
7.11846 kcal/mol |
270 293@LB@ | |@LB@5'-UAU...GCCGG U A CAUAGUAG-3'@LB@ UUUC UCGACAGGACAU UGAU@LB@ :||| ||:||||||||| |||:@LB@ GAAG AGUUGUCCUGUA ACUG@LB@3'-AUU...AAUGA U UAACA...CAA-5'@LB@ | |@LB@ 166 144@LB@@LB@@LB@Energy -14.80 kcal/mol@LB@Hybridization Energy -24.6442 kcal/mol@LB@Unfolding Energy - mRNA 4.4819 kcal/mol@LB@Unfolding Energy - sRNA 7.11846 kcal/mol@LB@@LB@Position - mRNA 271 -- 292@LB@Position - sRNA 145 -- 165@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 149 -- 155@LB@ |
| 135 |
0.00631 |
0.1741 |
bsu31510 |
yufK |
-14.38 |
0.044651 |
233 -- 280 |
42 -- 85 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________232________________________________________________281\n_____________|__________________________________________________|\n5'-UGC...UUUCC___GA_________CU___UUUU___U____CCUU_________AAC___ACUUU...UGC-3'\n______________GCU___UUGCGAUC___GC____UAG_UCGU____AUCCAUCUC___UUC\n______________|||___||||:|||___||____:|:_||||____|:|||||||___|||\n______________CGA___AACGUUAG___CG____GUU_AGCA____UGGGUAGAG___AAG\n3'-AUU...CUUAC___AGC________AAC__UUU______________________AAU___GGGUA...CAA-5'\n_____________|__________________________________________________|\n_____________86_________________________________________________41\n |
268 -- 274 |
48 -- 54 |
-29.5205 kcal/mol |
6.02241 kcal/mol |
9.1918 kcal/mol |
232 281@LB@ | |@LB@5'-UGC...UUUCC GA CU UUUU U CCUU AAC ACUUU...UGC-3'@LB@ GCU UUGCGAUC GC UAG UCGU AUCCAUCUC UUC@LB@ ||| ||||:||| || :|: |||| |:||||||| |||@LB@ CGA AACGUUAG CG GUU AGCA UGGGUAGAG AAG@LB@3'-AUU...CUUAC AGC AAC UUU AAU GGGUA...CAA-5'@LB@ | |@LB@ 86 41@LB@@LB@@LB@Energy -14.38 kcal/mol@LB@Hybridization Energy -29.5205 kcal/mol@LB@Unfolding Energy - mRNA 6.02241 kcal/mol@LB@Unfolding Energy - sRNA 9.1918 kcal/mol@LB@@LB@Position - mRNA 233 -- 280@LB@Position - sRNA 42 -- 85@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 191 |
0.008727 |
0.1751 |
bsu31280 |
yugU |
-11.72 |
0.152832 |
2 -- 31 |
120 -- 152 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________1___________________________________32\n_____________|___________________________________|\n__________5'-A_______AA__GA______________________CAUCA...GCG-3'\n______________AUAUUGA__CA__G_CCGCA_CAAG___CAUAUGA\n______________|:|||||__||__|_|||||_||||___|||||:|\n______________UGUAACU__GU__C_GGCGU_GUUC___GUAUAUU\n3'-AUU...UGUCC___________AA_A_____C____AAG_______ACAAC...CAA-5'\n_____________|___________________________________|\n_____________153_________________________________119\n |
25 -- 31 |
120 -- 126 |
-21.956 kcal/mol |
3.20784 kcal/mol |
9.17624 kcal/mol |
1 32@LB@ | |@LB@ 5'-A AA GA CAUCA...GCG-3'@LB@ AUAUUGA CA G CCGCA CAAG CAUAUGA@LB@ |:||||| || | ||||| |||| |||||:|@LB@ UGUAACU GU C GGCGU GUUC GUAUAUU@LB@3'-AUU...UGUCC AA A C AAG ACAAC...CAA-5'@LB@ | |@LB@ 153 119@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -21.956 kcal/mol@LB@Unfolding Energy - mRNA 3.20784 kcal/mol@LB@Unfolding Energy - sRNA 9.17624 kcal/mol@LB@@LB@Position - mRNA 2 -- 31@LB@Position - sRNA 120 -- 152@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 86 |
0.003162 |
0.1423 |
bsu32080 |
yuiB |
-17.14 |
0.011766 |
214 -- 271 |
6 -- 63 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________213____________________________________________________________272\n_____________|______________________________________________________________|\n5'-UAG...UCUGC________A_______AA_______G_U____GUC___UUU_______U_____________AUUAA...GGA-3'\n______________CAGUCGUA_UCAUCUC__UUGUUUU_U_UUUU___UUG___UUUGGAA_CG____GUUUCUU\n______________||:|||||_:||||||__||:||::_:_|||:___|||___||||:||_||____:||||||\n______________GUUAGCAU_GGUAGAG__AAUAAGG_G_AAAG___AAC___AAACUUU_GC____UAAAGAA\n3'-AUU...CGUUU________G________________G_U____A_____UUU_______U__GAAU_______AACAA-5'\n_____________|______________________________________________________________|\n_____________64_____________________________________________________________5\n |
265 -- 271 |
6 -- 12 |
-35.4392 kcal/mol |
4.76592 kcal/mol |
13.9327 kcal/mol |
213 272@LB@ | |@LB@5'-UAG...UCUGC A AA G U GUC UUU U AUUAA...GGA-3'@LB@ CAGUCGUA UCAUCUC UUGUUUU U UUUU UUG UUUGGAA CG GUUUCUU@LB@ ||:||||| :|||||| ||:||:: : |||: ||| ||||:|| || :||||||@LB@ GUUAGCAU GGUAGAG AAUAAGG G AAAG AAC AAACUUU GC UAAAGAA@LB@3'-AUU...CGUUU G G U A UUU U GAAU AACAA-5'@LB@ | |@LB@ 64 5@LB@@LB@@LB@Energy -17.14 kcal/mol@LB@Hybridization Energy -35.4392 kcal/mol@LB@Unfolding Energy - mRNA 4.76592 kcal/mol@LB@Unfolding Energy - sRNA 13.9327 kcal/mol@LB@@LB@Position - mRNA 214 -- 271@LB@Position - sRNA 6 -- 63@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 6 -- 12@LB@ |
| 100 |
0.004029 |
0.1561 |
bsu32980 |
yusZ |
-14.16 |
0.049671 |
3 -- 55 |
39 -- 90 |
oxidoreductase |
|
_____________2______________________________________56\n_____________|______________________________________|\n5'-CCG...GUCAU_____GUU_______GU____AUAG_____________AAUAG...CAA-3'\n______________UUCGU___CAGGAUA___ACA____CUGUAGCA_GUUU\n______________|||:|___|||||:|___|||____|:|:||||_|||:\n______________AAGUA___GUCCUGU___UGU____GGCGUCGU_CAAG\n3'-AUU...AUGAG_____GUU_______AAC___AACA________U____GUAUA...CAA-5'\n_____________|______________________________________|\n_____________91_____________________________________38\n |
175 -- 181 |
132 -- 138 |
-30.394 kcal/mol |
8.27302 kcal/mol |
10.1352 kcal/mol |
2 56@LB@ | |@LB@5'-CCG...GUCAU GUU GU AUAG AAUAG...CAA-3'@LB@ UUCGU CAGGAUA ACA CUGUAGCA GUUU@LB@ |||:| |||||:| ||| |:|:|||| |||:@LB@ AAGUA GUCCUGU UGU GGCGUCGU CAAG@LB@3'-AUU...AUGAG GUU AAC AACA U GUAUA...CAA-5'@LB@ | |@LB@ 91 38@LB@@LB@@LB@Energy -14.16 kcal/mol@LB@Hybridization Energy -30.394 kcal/mol@LB@Unfolding Energy - mRNA 8.27302 kcal/mol@LB@Unfolding Energy - sRNA 10.1352 kcal/mol@LB@@LB@Position - mRNA 3 -- 55@LB@Position - sRNA 39 -- 90@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 132 -- 138@LB@ |
| 168 |
0.007777 |
0.1751 |
bsu32270 |
yutH |
-11.70 |
0.153975 |
226 -- 259 |
121 -- 158 |
endospore coat-associated protein YutH |
|
_____________225____________________________________260\n_____________|______________________________________|\n5'-UGC...AGAAA____C______C____CAG_______U___________CCAGC...ACU-3'\n______________AAUA_GGCAUU_ACAU_____GCAGC___UUCCAUGUA\n______________||:|_|:||||_||||_____|||||___||||||:||\n______________UUGU_CUGUAA_UGUA_____CGUCG___AAGGUAUAU\n3'-AUU...AGUAG____C______C____ACAGG_____UUC_________UACAA...CAA-5'\n_____________|______________________________________|\n_____________159____________________________________120\n |
253 -- 259 |
121 -- 127 |
-28.4364 kcal/mol |
6.47482 kcal/mol |
10.499 kcal/mol |
225 260@LB@ | |@LB@5'-UGC...AGAAA C C CAG U CCAGC...ACU-3'@LB@ AAUA GGCAUU ACAU GCAGC UUCCAUGUA@LB@ ||:| |:|||| |||| ||||| ||||||:||@LB@ UUGU CUGUAA UGUA CGUCG AAGGUAUAU@LB@3'-AUU...AGUAG C C ACAGG UUC UACAA...CAA-5'@LB@ | |@LB@ 159 120@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -28.4364 kcal/mol@LB@Unfolding Energy - mRNA 6.47482 kcal/mol@LB@Unfolding Energy - sRNA 10.499 kcal/mol@LB@@LB@Position - mRNA 226 -- 259@LB@Position - sRNA 121 -- 158@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 27 |
0.0007779 |
0.1059 |
bsu31480 |
yuxJ |
-17.06 |
0.012234 |
48 -- 110 |
4 -- 55 |
MFS transporter |
|
_____________47______________________________________________________________111\n_____________|_______________________________________________________________|\n5'-UGC...AAUUC___U____GACAU___________CUAA____AA____UUUUCCCAGC_____C_________AAAUU...UUG-3'\n______________CCC_UCUC_____UUCUUCAUUUU____AAAU__UGAA__________CGCUU_GUUUCUUUU\n______________|||_||||_____|||::|||||:____||||__||||__________|||||_:||||||||\n______________GGG_AGAG_____AAGGGGUAAAG____UUUA__ACUU__________GCGAA_UAAAGAAAA\n3'-AUU...AGCAU___U____AAU_____________AAAC____A_____UU_____________U_________CAA-5'\n_____________|_______________________________________________________________|\n_____________56______________________________________________________________3\n |
104 -- 110 |
4 -- 10 |
-33.1771 kcal/mol |
7.88293 kcal/mol |
11.7347 kcal/mol |
47 111@LB@ | |@LB@5'-UGC...AAUUC U GACAU CUAA AA UUUUCCCAGC C AAAUU...UUG-3'@LB@ CCC UCUC UUCUUCAUUUU AAAU UGAA CGCUU GUUUCUUUU@LB@ ||| |||| |||::|||||: |||| |||| ||||| :||||||||@LB@ GGG AGAG AAGGGGUAAAG UUUA ACUU GCGAA UAAAGAAAA@LB@3'-AUU...AGCAU U AAU AAAC A UU U CAA-5'@LB@ | |@LB@ 56 3@LB@@LB@@LB@Energy -17.06 kcal/mol@LB@Hybridization Energy -33.1771 kcal/mol@LB@Unfolding Energy - mRNA 7.88293 kcal/mol@LB@Unfolding Energy - sRNA 11.7347 kcal/mol@LB@@LB@Position - mRNA 48 -- 110@LB@Position - sRNA 4 -- 55@LB@Position Seed - mRNA 104 -- 110@LB@Position Seed - sRNA 4 -- 10@LB@ |
| 111 |
0.004819 |
0.1671 |
bsu32120 |
yuzG |
-7.22 |
0.752372 |
165 -- 182 |
149 -- 166 |
Evidence 5: No homology to any previously reported sequences |
|
_____________164______________________________________183\n_____________|________________________________________|\n5'-UAG...UCUUU_____A_G_ACA__G__AAG__A________GAUG_____AAACG...UGG-3'\n______________AUGGC_U_G___UG_GA____G_GCGAAUAA____GCCCA\n______________|||||_|_|___||_:|____|_||:||:||____:||||\n______________UACCG_A_C___AC_UU____C_CGUUUGUU____UGGGU\n3'-AUU...UUACU_____A_G_A____G__AGAA__________AGCA_____AGAGA...CAA-5'\n_____________|________________________________________|\n_____________167______________________________________148\n |
192 -- 198 |
62 -- 68 |
-24.2214 kcal/mol |
8.24153 kcal/mol |
10.1588 kcal/mol |
164 183@LB@ | |@LB@5'-UAG...UCUUU A G ACA G AAG A GAUG AAACG...UGG-3'@LB@ AUGGC U G UG GA G GCGAAUAA GCCCA@LB@ ||||| | | || :| | ||:||:|| :||||@LB@ UACCG A C AC UU C CGUUUGUU UGGGU@LB@3'-AUU...UUACU A G A G AGAA AGCA AGAGA...CAA-5'@LB@ | |@LB@ 167 148@LB@@LB@@LB@Energy -7.22 kcal/mol@LB@Hybridization Energy -24.2214 kcal/mol@LB@Unfolding Energy - mRNA 8.24153 kcal/mol@LB@Unfolding Energy - sRNA 10.1588 kcal/mol@LB@@LB@Position - mRNA 165 -- 182@LB@Position - sRNA 149 -- 166@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 11 |
0.0003253 |
0.1011 |
bsu31319 |
yuzI |
-20.00 |
0.002830 |
16 -- 82 |
99 -- 165 |
Evidence 4: Homologs of previously reported genes of unknown function; PubMedId: 16704729; Product type f : factor |
|
_____________15______________________________________________________________________83\n_____________|_______________________________________________________________________|\n5'-UAA...CAGUC_____U___UUC__U_G___________G_U____U______UUUUCCUCACUC_________A__G____CAUCA...ACU-3'\n______________CUUCA_UCA___GG_C_AUUG_C__UGU_C_CAGC__GUUCC____________GUCACUUUC_UA_CUUC\n______________|||||_|||___||_|_||||_|__|||_|_||||__|||||____________|||||||||_:|_||||\n______________GAAGU_AGU___CC_G_UAAC_G__ACA_G_GUCG__CAAGG____________CAGUGAAAG_GU_GAAG\n3'-AUU...AAUGA_________UGU__U______U_UA___G_C____UU_____UAUAUUACAA___________A__A____UUUCA...CAA-5'\n_____________|_______________________________________________________________________|\n_____________166_____________________________________________________________________98\n |
68 -- 74 |
107 -- 113 |
-43.9371 kcal/mol |
8.77558 kcal/mol |
17.9339 kcal/mol |
15 83@LB@ | |@LB@5'-UAA...CAGUC U UUC U G G U U UUUUCCUCACUC A G CAUCA...ACU-3'@LB@ CUUCA UCA GG C AUUG C UGU C CAGC GUUCC GUCACUUUC UA CUUC@LB@ ||||| ||| || | |||| | ||| | |||| ||||| ||||||||| :| ||||@LB@ GAAGU AGU CC G UAAC G ACA G GUCG CAAGG CAGUGAAAG GU GAAG@LB@3'-AUU...AAUGA UGU U U UA G C UU UAUAUUACAA A A UUUCA...CAA-5'@LB@ | |@LB@ 166 98@LB@@LB@@LB@Energy -20.00 kcal/mol@LB@Hybridization Energy -43.9371 kcal/mol@LB@Unfolding Energy - mRNA 8.77558 kcal/mol@LB@Unfolding Energy - sRNA 17.9339 kcal/mol@LB@@LB@Position - mRNA 16 -- 82@LB@Position - sRNA 99 -- 165@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 107 -- 113@LB@ |
