| 60 |
0.001877 |
0.1212 |
bsu12040 |
yjdG |
-19.63 |
0.003403 |
61 -- 106 |
39 -- 81 |
N-acetyltransferase YjdG |
|
_____________60_______________________________________________107\n_____________|________________________________________________|\n5'-ACC...CUACC____UUU_____G_______UC___________AC____UCAA_____CUCAA...UAG-3'\n______________CGUU___GGUCU_GGCAAAC__UC__ACCUGUC__UCUU____UCCCC\n______________||||___::|||_|||||||__||__|||::||__||||____|||||\n______________GCAA___UUAGA_CCGUUUG__AG__UGGGUAG__AGAA____AGGGG\n3'-AUU...CCGAA____CG______A_______UU__CA_____________UA_______UAAAG...CAA-5'\n_____________|________________________________________________|\n_____________82_______________________________________________38\n |
85 -- 91 |
50 -- 56 |
-36.328 kcal/mol |
6.99682 kcal/mol |
13.0865 kcal/mol |
60 107@LB@ | |@LB@5'-ACC...CUACC UUU G UC AC UCAA CUCAA...UAG-3'@LB@ CGUU GGUCU GGCAAAC UC ACCUGUC UCUU UCCCC@LB@ |||| ::||| ||||||| || |||::|| |||| |||||@LB@ GCAA UUAGA CCGUUUG AG UGGGUAG AGAA AGGGG@LB@3'-AUU...CCGAA CG A UU CA UA UAAAG...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -19.63 kcal/mol@LB@Hybridization Energy -36.328 kcal/mol@LB@Unfolding Energy - mRNA 6.99682 kcal/mol@LB@Unfolding Energy - sRNA 13.0865 kcal/mol@LB@@LB@Position - mRNA 61 -- 106@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 4 |
0.0001192 |
0.08823 |
bsu12150 |
yjgB |
-21.60 |
0.001260 |
37 -- 72 |
32 -- 79 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 1580552 |
|
_____________36_______________________________________________73\n_____________|________________________________________________|\n5'-CCU...UGUGA____UU_____C____________________C_______________GCUUU...CAG-3'\n______________UUGU__UCUUG_CAG__________CCAUCUC__AUUCCCC_UUUCUU\n______________|||:__|||||_||:__________|||||||__|||||||_||||||\n______________AACG__AGAAC_GUU__________GGUAGAG__UAAGGGG_AAAGAA\n3'-AUU...GAAGC____UU_____C___UGUUAGCAUG_______AA_______U______ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________80_______________________________________________31\n |
60 -- 66 |
39 -- 45 |
-34.5565 kcal/mol |
2.03428 kcal/mol |
13.9015 kcal/mol |
36 73@LB@ | |@LB@5'-CCU...UGUGA UU C C GCUUU...CAG-3'@LB@ UUGU UCUUG CAG CCAUCUC AUUCCCC UUUCUU@LB@ |||: ||||| ||: ||||||| ||||||| ||||||@LB@ AACG AGAAC GUU GGUAGAG UAAGGGG AAAGAA@LB@3'-AUU...GAAGC UU C UGUUAGCAUG AA U ACUUU...CAA-5'@LB@ | |@LB@ 80 31@LB@@LB@@LB@Energy -21.60 kcal/mol@LB@Hybridization Energy -34.5565 kcal/mol@LB@Unfolding Energy - mRNA 2.03428 kcal/mol@LB@Unfolding Energy - sRNA 13.9015 kcal/mol@LB@@LB@Position - mRNA 37 -- 72@LB@Position - sRNA 32 -- 79@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 79 |
0.002683 |
0.1316 |
bsu12250 |
yjkB |
-15.23 |
0.029777 |
154 -- 245 |
42 -- 131 |
ABC transporter ATP-binding protein |
|
_____________153__________________________________________________________246\n_____________|____________________________________________________________|\n5'-AUA...UGAAA___GA__A_______GGAA__________AAACACAAAACAUACC_G_______C_____AGAUC...AGG-3'\n______________GGU__UU_UUGUAAU____GCAAACGAUC________________A_CCAUCUC___UUC\n______________||:__|:_|||:|||____||||||:|||________________|_|||||||___|||\n______________CCG__AG_AACGUUA____CGUUUGUUAG________________U_GGUAGAG___AAG\n3'-AUU...ACUUA___A___C_______GAAC__________CA_______________G_______AAU___GGGUA...CAA-5'\n_____________|____________________________________________________________|\n_____________132__________________________________________________________41\n |
235 -- 241 |
48 -- 54 |
-30.736 kcal/mol |
10.3673 kcal/mol |
9.19615 kcal/mol |
153 246@LB@ | |@LB@5'-AUA...UGAAA GA A GGAA AAACACAAAACAUACC G C AGAUC...AGG-3'@LB@ GGU UU UUGUAAU GCAAACGAUC A CCAUCUC UUC@LB@ ||: |: |||:||| ||||||:||| | ||||||| |||@LB@ CCG AG AACGUUA CGUUUGUUAG U GGUAGAG AAG@LB@3'-AUU...ACUUA A C GAAC CA G AAU GGGUA...CAA-5'@LB@ | |@LB@ 132 41@LB@@LB@@LB@Energy -15.23 kcal/mol@LB@Hybridization Energy -30.736 kcal/mol@LB@Unfolding Energy - mRNA 10.3673 kcal/mol@LB@Unfolding Energy - sRNA 9.19615 kcal/mol@LB@@LB@Position - mRNA 154 -- 245@LB@Position - sRNA 42 -- 131@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 59 |
0.001868 |
0.1212 |
bsu12260 |
yjlA |
-14.41 |
0.044077 |
46 -- 133 |
103 -- 182 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pt: transporter' |
|
_____________45__________________________________________________________________134\n_____________|___________________________________________________________________|\n5'-CGG...UGUCU_____UC_______UUCACCUCCCUUUU______UCCACAUAUA_____UCG_____AAC_______UUUUC...GGA-3'\n______________CUCUU__CAUCAAU______________UUGUCU__________GCGGU_____CCA___AUGAUGU\n______________|||||__||||||:______________|||||:__________||:|:_____|||___||:||||\n______________GAGAA__GUAGUUG______________AACAGG__________CGUCG_____GGU___UAUUACA\n3'-AUU...AAAAU______________UCCUGUAACUGU_______________________UUCAA___A_________ACAGU...CAA-5'\n_____________|___________________________________________________________________|\n_____________183_________________________________________________________________102\n |
118 -- 124 |
117 -- 123 |
-27.4081 kcal/mol |
7.08838 kcal/mol |
10.6441 kcal/mol |
45 134@LB@ | |@LB@5'-CGG...UGUCU UC UUCACCUCCCUUUU UCCACAUAUA UCG AAC UUUUC...GGA-3'@LB@ CUCUU CAUCAAU UUGUCU GCGGU CCA AUGAUGU@LB@ ||||| ||||||: |||||: ||:|: ||| ||:||||@LB@ GAGAA GUAGUUG AACAGG CGUCG GGU UAUUACA@LB@3'-AUU...AAAAU UCCUGUAACUGU UUCAA A ACAGU...CAA-5'@LB@ | |@LB@ 183 102@LB@@LB@@LB@Energy -14.41 kcal/mol@LB@Hybridization Energy -27.4081 kcal/mol@LB@Unfolding Energy - mRNA 7.08838 kcal/mol@LB@Unfolding Energy - sRNA 10.6441 kcal/mol@LB@@LB@Position - mRNA 46 -- 133@LB@Position - sRNA 103 -- 182@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 117 -- 123@LB@ |
| 101 |
0.004166 |
0.1594 |
bsu11270 |
yjzD |
-8.07 |
0.607351 |
275 -- 290 |
119 -- 136 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________274________________291\n_____________|__________________|\n5'-UAG...AUGAA________UA________UUCAA...CAU-3'\n______________CAGC__GU__CGUAUAAU\n______________||||__||__|:||||||\n______________GUCG__CA__GUAUAUUA\n3'-AUU...CAGGC____UU__AG________CAACA...CAA-5'\n_____________|__________________|\n_____________137________________118\n |
284 -- 290 |
119 -- 125 |
-12.692 kcal/mol |
1.37877 kcal/mol |
3.96273 kcal/mol |
274 291@LB@ | |@LB@5'-UAG...AUGAA UA UUCAA...CAU-3'@LB@ CAGC GU CGUAUAAU@LB@ |||| || |:||||||@LB@ GUCG CA GUAUAUUA@LB@3'-AUU...CAGGC UU AG CAACA...CAA-5'@LB@ | |@LB@ 137 118@LB@@LB@@LB@Energy -8.07 kcal/mol@LB@Hybridization Energy -12.692 kcal/mol@LB@Unfolding Energy - mRNA 1.37877 kcal/mol@LB@Unfolding Energy - sRNA 3.96273 kcal/mol@LB@@LB@Position - mRNA 275 -- 290@LB@Position - sRNA 119 -- 136@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 119 -- 125@LB@ |
| 162 |
0.007531 |
0.1751 |
bsu14350 |
yknX |
-8.74 |
0.495402 |
179 -- 195 |
123 -- 140 |
efflux system component YknX |
|
_____________178________________196\n_____________|__________________|\n5'-UGA...UCAAU____________G_____AGCUC...CCG-3'\n______________UCUGUGGCGGGU__CAUA\n______________||:|::||::||__||||\n______________AGGCGUCGUUCA__GUAU\n3'-AUU...GUAAC____________AG____AUUAC...CAA-5'\n_____________|__________________|\n_____________141________________122\n |
184 -- 190 |
129 -- 135 |
-18.5785 kcal/mol |
5.82762 kcal/mol |
6.13212 kcal/mol |
178 196@LB@ | |@LB@5'-UGA...UCAAU G AGCUC...CCG-3'@LB@ UCUGUGGCGGGU CAUA@LB@ ||:|::||::|| ||||@LB@ AGGCGUCGUUCA GUAU@LB@3'-AUU...GUAAC AG AUUAC...CAA-5'@LB@ | |@LB@ 141 122@LB@@LB@@LB@Energy -8.74 kcal/mol@LB@Hybridization Energy -18.5785 kcal/mol@LB@Unfolding Energy - mRNA 5.82762 kcal/mol@LB@Unfolding Energy - sRNA 6.13212 kcal/mol@LB@@LB@Position - mRNA 179 -- 195@LB@Position - sRNA 123 -- 140@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 129 -- 135@LB@ |
| 2 |
6.323e-05 |
0.08823 |
bsu13370 |
ykoQ |
-21.23 |
0.001523 |
8 -- 146 |
40 -- 170 |
metallophosphoesterase |
|
_____________7_______________________________________________________________________________________________________________________________________________________147\n_____________|_______________________________________________________________________________________________________________________________________________________|\n____5'-CGUAACA______ACA____C__UUGU___UC____________U_____U_____GCCUC_________AAA_______A__CCUUUUCC__________CAAAU__U__AUCAUAU______C___UUUUCA__________ACCCAUUA______GCUAU...CAA-3'\n______________UUGCUU___CAUC_AU____UAU__UGAC____UCCG_CGGCA_GUUCU_____AUG_UGUCA____UUUAUU_UC______________CUUC_____CA_UC_______GACAAU_CGU______ACCUGUCUCU________AUUCCC\n______________||:||:___||||_|:____:||__||||____||||_|:|||_||||:_____|||_|||||____:|:||:_||______________||||_____||_||_______:|||||_|||______|||::|||||________||||||\n______________AAUGAG___GUAG_UG____GUA__ACUG____AGGC_GUCGU_CAAGG_____UAC_ACAGU____GAGUAG_AG______________GAAG_____GU_AG_______UUGUUA_GCA______UGGGUAGAGA________UAAGGG\n3'-AUU...AAAAA______AA_____U__UCCU_________UAAC__________U_____UAUAU___A_____GAAA______A__UUUCAUUACUUACC____CAAC___U__AACCGU___________________________A_____________GUAAA...CAA-5'\n_____________|_______________________________________________________________________________________________________________________________________________________|\n_____________171_____________________________________________________________________________________________________________________________________________________39\n |
126 -- 132 |
47 -- 53 |
-61.8379 kcal/mol |
13.5948 kcal/mol |
30.7766 kcal/mol |
7 147@LB@ | |@LB@ 5'-CGUAACA ACA C UUGU UC U U GCCUC AAA A CCUUUUCC CAAAU U AUCAUAU C UUUUCA ACCCAUUA GCUAU...CAA-3'@LB@ UUGCUU CAUC AU UAU UGAC UCCG CGGCA GUUCU AUG UGUCA UUUAUU UC CUUC CA UC GACAAU CGU ACCUGUCUCU AUUCCC@LB@ ||:||: |||| |: :|| |||| |||| |:||| ||||: ||| ||||| :|:||: || |||| || || :||||| ||| |||::||||| ||||||@LB@ AAUGAG GUAG UG GUA ACUG AGGC GUCGU CAAGG UAC ACAGU GAGUAG AG GAAG GU AG UUGUUA GCA UGGGUAGAGA UAAGGG@LB@3'-AUU...AAAAA AA U UCCU UAAC U UAUAU A GAAA A UUUCAUUACUUACC CAAC U AACCGU A GUAAA...CAA-5'@LB@ | |@LB@ 171 39@LB@@LB@@LB@Energy -21.23 kcal/mol@LB@Hybridization Energy -61.8379 kcal/mol@LB@Unfolding Energy - mRNA 13.5948 kcal/mol@LB@Unfolding Energy - sRNA 30.7766 kcal/mol@LB@@LB@Position - mRNA 8 -- 146@LB@Position - sRNA 40 -- 170@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 133 |
0.006226 |
0.1741 |
bsu14430 |
ykpA |
-10.78 |
0.228396 |
48 -- 105 |
75 -- 129 |
ABC transporter ATP-binding protein |
|
_____________47____________106\n_____________|_____________|\n5'-GUA...AAGUU_____________AAUAU...UUG-3'\n______________AUUUGAAGAUGUU\n______________|||||||:|:|:|\n______________UAAACUUUUGCGA\n3'-AUU...AACUU_____________AUUAA...CAA-5'\n_____________|_____________|\n_____________130___________74\n |
254 -- 260 |
15 -- 21 |
-11.9489 kcal/mol |
0.844332 kcal/mol |
3.96844 kcal/mol |
47 106@LB@ | |@LB@5'-GUA...AAGUU AAUAU...UUG-3'@LB@ AUUUGAAGAUGUU@LB@ |||||||:|:|:|@LB@ UAAACUUUUGCGA@LB@3'-AUU...AACUU AUUAA...CAA-5'@LB@ | |@LB@ 130 74@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -11.9489 kcal/mol@LB@Unfolding Energy - mRNA 0.844332 kcal/mol@LB@Unfolding Energy - sRNA 3.96844 kcal/mol@LB@@LB@Position - mRNA 48 -- 105@LB@Position - sRNA 75 -- 129@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 170 |
0.007871 |
0.1751 |
bsu13520 |
ykrP |
-11.14 |
0.196181 |
76 -- 136 |
99 -- 167 |
membrane-bound acyltransferase YkrP |
|
_____________75____________137\n_____________|_____________|\n5'-GGA...CCGUC_____A_______CUAAC...AUC-3'\n______________ACUUU_CAUCGAC\n______________|||:|_||||:||\n______________UGAGA_GUAGUUG\n3'-AUU...AAAAA_____A_______UCCUG...CAA-5'\n_____________|_____________|\n_____________168___________98\n |
91 -- 97 |
156 -- 162 |
-12.1838 kcal/mol |
0.966795 kcal/mol |
1.40592 kcal/mol |
75 137@LB@ | |@LB@5'-GGA...CCGUC A CUAAC...AUC-3'@LB@ ACUUU CAUCGAC@LB@ |||:| ||||:||@LB@ UGAGA GUAGUUG@LB@3'-AUU...AAAAA A UCCUG...CAA-5'@LB@ | |@LB@ 168 98@LB@@LB@@LB@Energy -11.14 kcal/mol@LB@Hybridization Energy -12.1838 kcal/mol@LB@Unfolding Energy - mRNA 0.966795 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 76 -- 136@LB@Position - sRNA 99 -- 167@LB@Position Seed - mRNA 91 -- 97@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 129 |
0.005948 |
0.1741 |
bsu13580 |
ykrV |
-12.37 |
0.114104 |
93 -- 132 |
31 -- 61 |
transaminase MtnE |
|
_____________92________________________________________133\n_____________|_________________________________________|\n5'-CAG...AAGAU________AUUU________UAACA_____AAUU_______ACUUU...ACG-3'\n______________AUCGU_CC____CAUCUUUU_____CCCCA____UUUCUUU\n______________|||||_||____|||||:||_____|||||____|||||||\n______________UAGCA_GG____GUAGAGAA_____GGGGU____AAAGAAA\n3'-AUU...UUUGU_____U______________UAA__________________CUUUA...CAA-5'\n_____________|_________________________________________|\n_____________62________________________________________30\n |
126 -- 132 |
31 -- 37 |
-24.5638 kcal/mol |
3.82436 kcal/mol |
10.4555 kcal/mol |
92 133@LB@ | |@LB@5'-CAG...AAGAU AUUU UAACA AAUU ACUUU...ACG-3'@LB@ AUCGU CC CAUCUUUU CCCCA UUUCUUU@LB@ ||||| || |||||:|| ||||| |||||||@LB@ UAGCA GG GUAGAGAA GGGGU AAAGAAA@LB@3'-AUU...UUUGU U UAA CUUUA...CAA-5'@LB@ | |@LB@ 62 30@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -24.5638 kcal/mol@LB@Unfolding Energy - mRNA 3.82436 kcal/mol@LB@Unfolding Energy - sRNA 10.4555 kcal/mol@LB@@LB@Position - mRNA 93 -- 132@LB@Position - sRNA 31 -- 61@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 31 -- 37@LB@ |
