| 136 |
0.006329 |
0.1741 |
bsu10210 |
yhfF |
-9.29 |
0.409483 |
117 -- 143 |
37 -- 59 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________116______________144\n_____________|________________|\n5'-AUA...GACGC_______GCA______CGCAC...CCA-3'\n______________UUCCCAU___UUUGAA\n______________|:|||||___||||||\n______________AGGGGUA___AAACUU\n3'-AUU...GAAUA_______AAG______UAAAC...CAA-5'\n_____________|________________|\n_____________60_______________36\n |
101 -- 107 |
37 -- 43 |
-14.7282 kcal/mol |
3.20386 kcal/mol |
5.02573 kcal/mol |
116 144@LB@ | |@LB@5'-AUA...GACGC GCA CGCAC...CCA-3'@LB@ UUCCCAU UUUGAA@LB@ |:||||| ||||||@LB@ AGGGGUA AAACUU@LB@3'-AUU...GAAUA AAG UAAAC...CAA-5'@LB@ | |@LB@ 60 36@LB@@LB@@LB@Energy -9.29 kcal/mol@LB@Hybridization Energy -14.7282 kcal/mol@LB@Unfolding Energy - mRNA 3.20386 kcal/mol@LB@Unfolding Energy - sRNA 5.02573 kcal/mol@LB@@LB@Position - mRNA 117 -- 143@LB@Position - sRNA 37 -- 59@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 43 |
0.00135 |
0.1206 |
bsu10230 |
yhfH |
-10.18 |
0.291514 |
18 -- 110 |
27 -- 139 |
Evidence 6: Doubtful CDS |
|
_____________17_____________________________________________________________________________________________________________________111\n_____________|______________________________________________________________________________________________________________________|\n5'-CAU...GAAAA____CAU________________C_____A______A________________UCCUC_______________UU____AA_____GU___CC___AAUAUA_________C______AAAGC...AUG-3'\n______________CCGC___AGCA___UCCG_AUAA___UGU_ACUUUC____________UAAUG_____CU________AUCUU__CAAA__AAUUG__CCU__UUC______CCCAUUUUU_UUGAAA\n______________||||___||||___|||:_||||___|||_||||||____________|||||_____||________|||||__||||__|||:|__||:__:||______|||||||:|_||||||\n______________GGCG___UCGU___AGGU_UAUU___ACA_UGAAAG____________AUUAC_____GA________UAGAA__GUUU__UUAGC__GGG__GAG______GGGUAAAGA_AACUUU\n3'-AUU...UAACA___________UCA____A____ACA___G______AGUAGAAGUUUC_____UUACC__AGCAACGU_____CC____G______AU___UA___AAUAAG________________AAACU...CAA-5'\n_____________|______________________________________________________________________________________________________________________|\n_____________140____________________________________________________________________________________________________________________26\n |
96 -- 102 |
34 -- 40 |
-47.8615 kcal/mol |
11.539 kcal/mol |
28.2686 kcal/mol |
17 111@LB@ | |@LB@5'-CAU...GAAAA CAU C A A UCCUC UU AA GU CC AAUAUA C AAAGC...AUG-3'@LB@ CCGC AGCA UCCG AUAA UGU ACUUUC UAAUG CU AUCUU CAAA AAUUG CCU UUC CCCAUUUUU UUGAAA@LB@ |||| |||| |||: |||| ||| |||||| ||||| || ||||| |||| |||:| ||: :|| |||||||:| ||||||@LB@ GGCG UCGU AGGU UAUU ACA UGAAAG AUUAC GA UAGAA GUUU UUAGC GGG GAG GGGUAAAGA AACUUU@LB@3'-AUU...UAACA UCA A ACA G AGUAGAAGUUUC UUACC AGCAACGU CC G AU UA AAUAAG AAACU...CAA-5'@LB@ | |@LB@ 140 26@LB@@LB@@LB@Energy -10.18 kcal/mol@LB@Hybridization Energy -47.8615 kcal/mol@LB@Unfolding Energy - mRNA 11.539 kcal/mol@LB@Unfolding Energy - sRNA 28.2686 kcal/mol@LB@@LB@Position - mRNA 18 -- 110@LB@Position - sRNA 27 -- 139@LB@Position Seed - mRNA 96 -- 102@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 31 |
0.0008564 |
0.1059 |
bsu10290 |
yhfN |
-10.46 |
0.260955 |
32 -- 88 |
131 -- 186 |
metalloprotease YhfN |
|
_____________31__________________89\n_____________|___________________|\n5'-AUG...GCAAG__________G________ACUUG...AAG-3'\n______________UGG_AUUG_C_GCAGCAGG\n______________||:_||||_|_||||||:|\n______________ACU_UAAC_G_CGUCGUUC\n3'-AUU...CUGUA___G____A_G________AAGGU...CAA-5'\n_____________|___________________|\n_____________187_________________130\n |
220 -- 226 |
130 -- 136 |
-18.5522 kcal/mol |
5.78917 kcal/mol |
6.22375 kcal/mol |
31 89@LB@ | |@LB@5'-AUG...GCAAG G ACUUG...AAG-3'@LB@ UGG AUUG C GCAGCAGG@LB@ ||: |||| | ||||||:|@LB@ ACU UAAC G CGUCGUUC@LB@3'-AUU...CUGUA G A G AAGGU...CAA-5'@LB@ | |@LB@ 187 130@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -18.5522 kcal/mol@LB@Unfolding Energy - mRNA 5.78917 kcal/mol@LB@Unfolding Energy - sRNA 6.22375 kcal/mol@LB@@LB@Position - mRNA 32 -- 88@LB@Position - sRNA 131 -- 186@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 102 |
0.004212 |
0.1594 |
bsu10510 |
yhjH |
-17.94 |
0.007893 |
238 -- 265 |
152 -- 183 |
MarR family transcriptional regulator |
|
_____________237________________266\n_____________|__________________|\n5'-GCA...GCGGA____A_____________GAAAU...CUG-3'\n______________GCUC_UUCAUCAGCAGGA\n______________:|||_|||||||:|||||\n______________UGAG_AAGUAGUUGUCCU\n3'-AUU...AAAAA__________________GUAAC...CAA-5'\n_____________|__________________|\n_____________184________________151\n |
259 -- 265 |
152 -- 158 |
-25.8948 kcal/mol |
5.0064 kcal/mol |
6.06644 kcal/mol |
237 266@LB@ | |@LB@5'-GCA...GCGGA A GAAAU...CUG-3'@LB@ GCUC UUCAUCAGCAGGA@LB@ :||| |||||||:|||||@LB@ UGAG AAGUAGUUGUCCU@LB@3'-AUU...AAAAA GUAAC...CAA-5'@LB@ | |@LB@ 184 151@LB@@LB@@LB@Energy -17.94 kcal/mol@LB@Hybridization Energy -25.8948 kcal/mol@LB@Unfolding Energy - mRNA 5.0064 kcal/mol@LB@Unfolding Energy - sRNA 6.06644 kcal/mol@LB@@LB@Position - mRNA 238 -- 265@LB@Position - sRNA 152 -- 183@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 152 -- 158@LB@ |
| 153 |
0.007014 |
0.1751 |
bsu10400 |
yhxC |
-16.66 |
0.014878 |
138 -- 167 |
138 -- 167 |
oxidoreductase |
|
_____________137____________________________168\n_____________|______________________________|\n5'-UCC...UCUAU________AC______AGCA__________AUAAC...CGA-3'\n______________CUUUUCAU__AUAGGA____GGCAUUGUUC\n______________||:|||||__|:||||____|:||||||:|\n______________GAGAAGUA__UGUCCU____CUGUAACAGG\n3'-AUU...AAAAU________GU______GUAA__________CGUCG...CAA-5'\n_____________|______________________________|\n_____________168____________________________137\n |
161 -- 167 |
138 -- 144 |
-29.0079 kcal/mol |
5.02609 kcal/mol |
7.37082 kcal/mol |
137 168@LB@ | |@LB@5'-UCC...UCUAU AC AGCA AUAAC...CGA-3'@LB@ CUUUUCAU AUAGGA GGCAUUGUUC@LB@ ||:||||| |:|||| |:||||||:|@LB@ GAGAAGUA UGUCCU CUGUAACAGG@LB@3'-AUU...AAAAU GU GUAA CGUCG...CAA-5'@LB@ | |@LB@ 168 137@LB@@LB@@LB@Energy -16.66 kcal/mol@LB@Hybridization Energy -29.0079 kcal/mol@LB@Unfolding Energy - mRNA 5.02609 kcal/mol@LB@Unfolding Energy - sRNA 7.37082 kcal/mol@LB@@LB@Position - mRNA 138 -- 167@LB@Position - sRNA 138 -- 167@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 138 -- 144@LB@ |
| 167 |
0.007775 |
0.1751 |
bsu09889 |
yhzD |
-15.75 |
0.023145 |
43 -- 92 |
39 -- 89 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________42____________________________________________________93\n_____________|_____________________________________________________|\n5'-GGA...CUCCA____U_______AC______AAUU_______C___A____C____C_______UGACC...GUG-3'\n______________AAUG_CUUCGUU__GUGAUC____GUAAGCA__UG_AUCU__CUC___UUCCC\n______________||||_|||||||__|::|||____|:||:||__:|_|:|:__|||___|||||\n______________UUAC_GAAGCAA__CGUUAG____CGUUUGU__GC_UGGG__GAG___AAGGG\n3'-AUU...AUUAC____C_______________AAC________UA__A____UA___AAU_____GUAAA...CAA-5'\n_____________|_____________________________________________________|\n_____________90____________________________________________________38\n |
67 -- 73 |
62 -- 68 |
-30.4166 kcal/mol |
5.34264 kcal/mol |
10.926 kcal/mol |
42 93@LB@ | |@LB@5'-GGA...CUCCA U AC AAUU C A C C UGACC...GUG-3'@LB@ AAUG CUUCGUU GUGAUC GUAAGCA UG AUCU CUC UUCCC@LB@ |||| ||||||| |::||| |:||:|| :| |:|: ||| |||||@LB@ UUAC GAAGCAA CGUUAG CGUUUGU GC UGGG GAG AAGGG@LB@3'-AUU...AUUAC C AAC UA A UA AAU GUAAA...CAA-5'@LB@ | |@LB@ 90 38@LB@@LB@@LB@Energy -15.75 kcal/mol@LB@Hybridization Energy -30.4166 kcal/mol@LB@Unfolding Energy - mRNA 5.34264 kcal/mol@LB@Unfolding Energy - sRNA 10.926 kcal/mol@LB@@LB@Position - mRNA 43 -- 92@LB@Position - sRNA 39 -- 89@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 72 |
0.002326 |
0.1242 |
bsu10730 |
yisI |
-10.35 |
0.272902 |
44 -- 108 |
96 -- 182 |
aspartyl-phosphate phosphatase YisI |
|
_____________43___________________________________109\n_____________|____________________________________|\n5'-UAG...AAAGG_________AAA________________________GUCAA...UGG-3'\n______________CCGCAGCAA_____________UUGUCGCUUUUUUA\n______________|||||||||_____________|||||:||||:|:|\n______________GGCGUCGUU_____________AACAGUGAAAGAGU\n3'-AUU...UAACA_________CAAGGUAUAUUAC______________AGAAG...CAA-5'\n_____________|____________________________________|\n_____________183__________________________________95\n |
90 -- 96 |
110 -- 116 |
-28.3207 kcal/mol |
8.15126 kcal/mol |
10.3722 kcal/mol |
43 109@LB@ | |@LB@5'-UAG...AAAGG AAA GUCAA...UGG-3'@LB@ CCGCAGCAA UUGUCGCUUUUUUA@LB@ ||||||||| |||||:||||:|:|@LB@ GGCGUCGUU AACAGUGAAAGAGU@LB@3'-AUU...UAACA CAAGGUAUAUUAC AGAAG...CAA-5'@LB@ | |@LB@ 183 95@LB@@LB@@LB@Energy -10.35 kcal/mol@LB@Hybridization Energy -28.3207 kcal/mol@LB@Unfolding Energy - mRNA 8.15126 kcal/mol@LB@Unfolding Energy - sRNA 10.3722 kcal/mol@LB@@LB@Position - mRNA 44 -- 108@LB@Position - sRNA 96 -- 182@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 110 -- 116@LB@ |
| 6 |
0.0001543 |
0.08823 |
bsu10740 |
yisJ |
-16.00 |
0.020473 |
36 -- 139 |
34 -- 134 |
inner spore coat protein H-like protein |
|
_____________35____________________________________________________140\n_____________|_____________________________________________________|\n5'-GGC...GUUCC______GCAAAUUUCAUG_______U____CA_____U____C__________CCUUC...UUC-3'\n______________CCUGUC____________UUAUUCC_CCAU__UCUUU_AAAU___AGAAUGCU\n______________||::||____________|||||||_||||__|||||_||||___|:||:|||\n______________GGGUAG____________AAUAAGG_GGUA__AGAAA_UUUA___UUUUGCGA\n3'-AUU...AGCAU______AG______________________A______C____AAC________AUUAA...CAA-5'\n_____________|_____________________________________________________|\n_____________135___________________________________________________33\n |
74 -- 80 |
15 -- 21 |
-28.1573 kcal/mol |
3.71522 kcal/mol |
11.7008 kcal/mol |
35 140@LB@ | |@LB@5'-GGC...GUUCC GCAAAUUUCAUG U CA U C CCUUC...UUC-3'@LB@ CCUGUC UUAUUCC CCAU UCUUU AAAU AGAAUGCU@LB@ ||::|| ||||||| |||| ||||| |||| |:||:|||@LB@ GGGUAG AAUAAGG GGUA AGAAA UUUA UUUUGCGA@LB@3'-AUU...AGCAU AG A C AAC AUUAA...CAA-5'@LB@ | |@LB@ 135 33@LB@@LB@@LB@Energy -16.00 kcal/mol@LB@Hybridization Energy -28.1573 kcal/mol@LB@Unfolding Energy - mRNA 3.71522 kcal/mol@LB@Unfolding Energy - sRNA 11.7008 kcal/mol@LB@@LB@Position - mRNA 36 -- 139@LB@Position - sRNA 34 -- 134@LB@Position Seed - mRNA 74 -- 80@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 107 |
0.004539 |
0.1633 |
bsu11020 |
yitK |
-8.14 |
0.595243 |
151 -- 171 |
155 -- 171 |
nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription |
|
_____________150___________________172\n_____________|_____________________|\n5'-ACC...UUCUC________ACUAUA_______CAUAA...AUG-3'\n______________UUUAUUCU______AUCAACA\n______________||||:|||______|||||||\n______________AAAUGAGA______UAGUUGU\n3'-AUU...AAAAA________AG___________CCUGU...CAA-5'\n_____________|_____________________|\n_____________172___________________154\n |
165 -- 171 |
155 -- 161 |
-10.3591 kcal/mol |
1.70839 kcal/mol |
1.4241 kcal/mol |
150 172@LB@ | |@LB@5'-ACC...UUCUC ACUAUA CAUAA...AUG-3'@LB@ UUUAUUCU AUCAACA@LB@ ||||:||| |||||||@LB@ AAAUGAGA UAGUUGU@LB@3'-AUU...AAAAA AG CCUGU...CAA-5'@LB@ | |@LB@ 172 154@LB@@LB@@LB@Energy -8.14 kcal/mol@LB@Hybridization Energy -10.3591 kcal/mol@LB@Unfolding Energy - mRNA 1.70839 kcal/mol@LB@Unfolding Energy - sRNA 1.4241 kcal/mol@LB@@LB@Position - mRNA 151 -- 171@LB@Position - sRNA 155 -- 171@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 155 -- 161@LB@ |
| 160 |
0.007403 |
0.1751 |
bsu11139 |
yizC |
-17.39 |
0.010400 |
11 -- 81 |
42 -- 107 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________10____________________________________________________________________________82\n_____________|_____________________________________________________________________________|\n5'-UUG...GACGA_______CG________UG______GG____________CAAAAUG_________AGCAA_____GAAGG_______AGCAG...UGA-3'\n______________CUCAUCU__UCAAAGUA__GAA_GG_______GCAGUCU_______GGUAAACGA_____AUCCA_____UUUAUUC\n______________|||||||__||||||||__|||_||_______|||:|||_______||:||||:|_____|:|||_____:||||||\n______________GAGUAGA__AGUUUCAU__CUU_CC_______CGUUAGA_______CCGUUUGUU_____UGGGU_____GAAUAAG\n3'-AUU...UGAAA_________________UA___A__GAAGCAA_______A_______________AGCA______AGA_________GGGUA...CAA-5'\n_____________|_____________________________________________________________________________|\n_____________108___________________________________________________________________________41\n |
75 -- 81 |
42 -- 48 |
-46.4368 kcal/mol |
15.8344 kcal/mol |
18.2654 kcal/mol |
10 82@LB@ | |@LB@5'-UUG...GACGA CG UG GG CAAAAUG AGCAA GAAGG AGCAG...UGA-3'@LB@ CUCAUCU UCAAAGUA GAA GG GCAGUCU GGUAAACGA AUCCA UUUAUUC@LB@ ||||||| |||||||| ||| || |||:||| ||:||||:| |:||| :||||||@LB@ GAGUAGA AGUUUCAU CUU CC CGUUAGA CCGUUUGUU UGGGU GAAUAAG@LB@3'-AUU...UGAAA UA A GAAGCAA A AGCA AGA GGGUA...CAA-5'@LB@ | |@LB@ 108 41@LB@@LB@@LB@Energy -17.39 kcal/mol@LB@Hybridization Energy -46.4368 kcal/mol@LB@Unfolding Energy - mRNA 15.8344 kcal/mol@LB@Unfolding Energy - sRNA 18.2654 kcal/mol@LB@@LB@Position - mRNA 11 -- 81@LB@Position - sRNA 42 -- 107@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 42 -- 48@LB@ |
