| 5 |
0.0001198 |
0.08823 |
bsu33680 |
yvaP |
-23.88 |
0.000393 |
26 -- 134 |
34 -- 167 |
HTH-type transcriptional regulator YvaP |
|
_____________25___________________________________________________________________________________________________________________________________________________________135\n_____________|____________________________________________________________________________________________________________________________________________________________|\n5'-UUU...AACAU_______A______________AAA____________AUA________________A_______________AGCAAAAAAAC__________C_____C__UC______C___________CU____A______AUCAAUC______________CAUGC...CGC-3'\n______________CUC_UCA__AGCAGGAUAUUGA_____UCU_CAGCAA___CU___________UCG_UUUUUUGUCUUCAGA___________GC______GC__UCUU__C__AUAAUC___CCUGUUUCU__UUCU_UAUUUU______________ACGCUUG\n______________|||_|||__|:|||||:|||||_____||:_||||||___|:___________||:_|||:|::||||||:|___________||______||__||||__|__|:||||___||::|:|||__|||:_:||||:______________||||||:\n______________GAG_AGU__UUGUCCUGUAACU_____AGG_GUCGUU___GG___________AGU_AAAGAGUAGAAGUUU___________CG______CG__AGAA__G__UGUUAG___GGGUAGAGA__AAGG_GUAAAG______________UGCGAAU\n3'-AUU...AAAAU___A___AG_____________GUAAC___C______CAA__UAUAUUACAAC___G_______________CAUUACUUAC___AAGCAA__UU____CC_UU______CAU_________AU____G______AAACUUUAAACUUU_______UAAAG...CAA-5'\n_____________|____________________________________________________________________________________________________________________________________________________________|\n_____________168__________________________________________________________________________________________________________________________________________________________33\n |
142 -- 148 |
13 -- 19 |
-70.4657 kcal/mol |
17.0109 kcal/mol |
33.4152 kcal/mol |
25 135@LB@ | |@LB@5'-UUU...AACAU A AAA AUA A AGCAAAAAAAC C C UC C CU A AUCAAUC CAUGC...CGC-3'@LB@ CUC UCA AGCAGGAUAUUGA UCU CAGCAA CU UCG UUUUUUGUCUUCAGA GC GC UCUU C AUAAUC CCUGUUUCU UUCU UAUUUU ACGCUUG@LB@ ||| ||| |:|||||:||||| ||: |||||| |: ||: |||:|::||||||:| || || |||| | |:|||| ||::|:||| |||: :||||: ||||||:@LB@ GAG AGU UUGUCCUGUAACU AGG GUCGUU GG AGU AAAGAGUAGAAGUUU CG CG AGAA G UGUUAG GGGUAGAGA AAGG GUAAAG UGCGAAU@LB@3'-AUU...AAAAU A AG GUAAC C CAA UAUAUUACAAC G CAUUACUUAC AAGCAA UU CC UU CAU AU G AAACUUUAAACUUU UAAAG...CAA-5'@LB@ | |@LB@ 168 33@LB@@LB@@LB@Energy -23.88 kcal/mol@LB@Hybridization Energy -70.4657 kcal/mol@LB@Unfolding Energy - mRNA 17.0109 kcal/mol@LB@Unfolding Energy - sRNA 33.4152 kcal/mol@LB@@LB@Position - mRNA 26 -- 134@LB@Position - sRNA 34 -- 167@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 16 |
0.0004911 |
0.1011 |
bsu34650 |
yvdC |
-21.31 |
0.001462 |
17 -- 94 |
101 -- 185 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pe: enzyme' |
|
_____________16_______________________________________________________________________95\n_____________|________________________________________________________________________|\n5'-AUA...UUUUA___G_______UCCCC_____U______C_________AAUACA____A________U____C__G______CCGCA...AGG-3'\n______________UAC_CUUCAUU_____CAGGG__UUGAU_____CCGCA________CA_AUG__GUU_UCAU_UU_UCAUCU\n______________|||_||||||:_____||||:__||||:_____|||||________||_||:__|||_|||:_||_||||||\n______________AUG_GAAGUAG_____GUCCU__AACUG_____GGCGU________GU_UAU__CAA_AGUG_AA_AGUAGA\n3'-AUU...AAAAA___A_______UU________GU_____UAACA_____CGUUCAAG__A___UA___C____A__G______AGUUU...CAA-5'\n_____________|________________________________________________________________________|\n_____________186______________________________________________________________________100\n |
36 -- 42 |
159 -- 165 |
-36.2616 kcal/mol |
8.0324 kcal/mol |
15.7762 kcal/mol |
16 95@LB@ | |@LB@5'-AUA...UUUUA G UCCCC U C AAUACA A U C G CCGCA...AGG-3'@LB@ UAC CUUCAUU CAGGG UUGAU CCGCA CA AUG GUU UCAU UU UCAUCU@LB@ ||| ||||||: ||||: ||||: ||||| || ||: ||| |||: || ||||||@LB@ AUG GAAGUAG GUCCU AACUG GGCGU GU UAU CAA AGUG AA AGUAGA@LB@3'-AUU...AAAAA A UU GU UAACA CGUUCAAG A UA C A G AGUUU...CAA-5'@LB@ | |@LB@ 186 100@LB@@LB@@LB@Energy -21.31 kcal/mol@LB@Hybridization Energy -36.2616 kcal/mol@LB@Unfolding Energy - mRNA 8.0324 kcal/mol@LB@Unfolding Energy - sRNA 15.7762 kcal/mol@LB@@LB@Position - mRNA 17 -- 94@LB@Position - sRNA 101 -- 185@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 159 -- 165@LB@ |
| 63 |
0.001974 |
0.1214 |
bsu34640 |
yvdD |
-9.92 |
0.323853 |
41 -- 65 |
33 -- 60 |
LOG family protein YvdD |
|
_____________40___________________________66\n_____________|____________________________|\n5'-GAC...GUAAA______UCAUCCAUUUUUCUG_______CAGCU...GAA-3'\n______________ACUCUU_______________CAUCAGC\n______________||||||_______________|||||:|\n______________UGAGAA_______________GUAGUUG\n3'-AUU...AAAAA____________________________UCCUG...CAA-5'\n_____________|____________________________|\n_____________61___________________________32\n |
69 -- 75 |
156 -- 162 |
-11.7297 kcal/mol |
2.56294 kcal/mol |
1.40592 kcal/mol |
40 66@LB@ | |@LB@5'-GAC...GUAAA UCAUCCAUUUUUCUG CAGCU...GAA-3'@LB@ ACUCUU CAUCAGC@LB@ |||||| |||||:|@LB@ UGAGAA GUAGUUG@LB@3'-AUU...AAAAA UCCUG...CAA-5'@LB@ | |@LB@ 61 32@LB@@LB@@LB@Energy -9.92 kcal/mol@LB@Hybridization Energy -11.7297 kcal/mol@LB@Unfolding Energy - mRNA 2.56294 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 41 -- 65@LB@Position - sRNA 33 -- 60@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 7 |
0.0001858 |
0.08823 |
bsu34210 |
yvfG |
-17.07 |
0.012126 |
85 -- 142 |
32 -- 86 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________84____________________________________________________________143\n_____________|_____________________________________________________________|\n5'-GAG...GUUGA______AAUAC___________C____GUU__A__________GCUCAC____________CAUUG...ACU-3'\n______________GGCUUU_____UUGUA__CUUG_UGAA___UC_UAUCCAUCUU______UCCCC_UUUCUU\n______________|||||:_____|||:|__||||_::||___||_||:||||||:______|||||_||||||\n______________CCGAAG_____AACGU__GAAC_GUUU___AG_AUGGGUAGAG______AGGGG_AAAGAA\n3'-AUU...ACUUA______C_________UA____C____GUU__C__________AAUA_______U______ACUUU...CAA-5'\n_____________|_____________________________________________________________|\n_____________87____________________________________________________________31\n |
119 -- 125 |
48 -- 54 |
-40.7387 kcal/mol |
11.2598 kcal/mol |
14.8836 kcal/mol |
84 143@LB@ | |@LB@5'-GAG...GUUGA AAUAC C GUU A GCUCAC CAUUG...ACU-3'@LB@ GGCUUU UUGUA CUUG UGAA UC UAUCCAUCUU UCCCC UUUCUU@LB@ |||||: |||:| |||| ::|| || ||:||||||: ||||| ||||||@LB@ CCGAAG AACGU GAAC GUUU AG AUGGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACUUA C UA C GUU C AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 87 31@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -40.7387 kcal/mol@LB@Unfolding Energy - mRNA 11.2598 kcal/mol@LB@Unfolding Energy - sRNA 14.8836 kcal/mol@LB@@LB@Position - mRNA 85 -- 142@LB@Position - sRNA 32 -- 86@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 119 |
0.005369 |
0.1741 |
bsu33250 |
yvrL |
-8.38 |
0.555183 |
10 -- 36 |
76 -- 105 |
membrane-bound negative regulator YvrL |
|
_____________9__________37\n_____________|__________|\n5'-AUG...CCUUC__________UUGCG...AUA-3'\n______________AAAACGCUUA\n______________||||||||||\n______________UUUUGCGAAU\n3'-AUU...UAAAC__________UAAAG...CAA-5'\n_____________|__________|\n_____________106________75\n |
224 -- 230 |
13 -- 19 |
-10.8458 kcal/mol |
2.09283 kcal/mol |
3.31287 kcal/mol |
9 37@LB@ | |@LB@5'-AUG...CCUUC UUGCG...AUA-3'@LB@ AAAACGCUUA@LB@ ||||||||||@LB@ UUUUGCGAAU@LB@3'-AUU...UAAAC UAAAG...CAA-5'@LB@ | |@LB@ 106 75@LB@@LB@@LB@Energy -8.38 kcal/mol@LB@Hybridization Energy -10.8458 kcal/mol@LB@Unfolding Energy - mRNA 2.09283 kcal/mol@LB@Unfolding Energy - sRNA 3.31287 kcal/mol@LB@@LB@Position - mRNA 10 -- 36@LB@Position - sRNA 76 -- 105@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 197 |
0.009002 |
0.1751 |
bsu33650 |
yvzC |
-16.51 |
0.015985 |
3 -- 52 |
121 -- 172 |
HTH-type transcriptional regulator YvzC |
|
_____________2__________________________________________________53\n_____________|__________________________________________________|\n____5'-ACUUUCU_______C_____AU__CC__UA_________CAA___U___________UCUCA...UCU-3'\n______________CUCUUCA___GCA__GC___G__AUUGUUCGC___AGC___UUCCAUAUA\n______________|||||||___:||__:|___|__|||||:|||___|||___|||||||||\n______________GAGAAGU___UGU__UG___C__UAACAGGCG___UCG___AAGGUAUAU\n3'-AUU...AAAAU_______AGU___CC__UAA_UG_______________UUC_________UACAA...CAA-5'\n_____________|__________________________________________________|\n_____________173________________________________________________120\n |
46 -- 52 |
121 -- 127 |
-33.6584 kcal/mol |
6.173 kcal/mol |
10.5345 kcal/mol |
2 53@LB@ | |@LB@ 5'-ACUUUCU C AU CC UA CAA U UCUCA...UCU-3'@LB@ CUCUUCA GCA GC G AUUGUUCGC AGC UUCCAUAUA@LB@ ||||||| :|| :| | |||||:||| ||| |||||||||@LB@ GAGAAGU UGU UG C UAACAGGCG UCG AAGGUAUAU@LB@3'-AUU...AAAAU AGU CC UAA UG UUC UACAA...CAA-5'@LB@ | |@LB@ 173 120@LB@@LB@@LB@Energy -16.51 kcal/mol@LB@Hybridization Energy -33.6584 kcal/mol@LB@Unfolding Energy - mRNA 6.173 kcal/mol@LB@Unfolding Energy - sRNA 10.5345 kcal/mol@LB@@LB@Position - mRNA 3 -- 52@LB@Position - sRNA 121 -- 172@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 50 |
0.001627 |
0.1212 |
bsu38130 |
ywcE |
-9.91 |
0.324646 |
1 -- 15 |
39 -- 56 |
spore morphogenesis and germination protein YwcE |
|
_____________0_______________________________________________________________16\n_____________|_______________________________________________________________|\n5'-GCC...AAAAA_____ACA______________A__A___A______U____________UU____U_______AUCAC...CAA-3'\n______________CAAUC_____AAACAG____AC_CA_CUC__GUUCC_CUAUUUC____A__UUUG_AAACGUU\n______________|||||_____|||||:____||_||_|||__:||||_|:|||||____|__||||_|||||:|\n______________GUUAG_____UUUGUU____UG_GU_GAG__UAAGG_GGUAAAG____U__AAAC_UUUGCGA\n3'-AUU...GCAAC_____AACCG______AGCA__G__A___AA_____________AAAC_UU____U_______AUUAA...CAA-5'\n_____________|_______________________________________________________________|\n_____________57______________________________________________________________38\n |
170 -- 176 |
15 -- 21 |
-29.8413 kcal/mol |
5.55001 kcal/mol |
15.9749 kcal/mol |
0 16@LB@ | |@LB@5'-GCC...AAAAA ACA A A A U UU U AUCAC...CAA-3'@LB@ CAAUC AAACAG AC CA CUC GUUCC CUAUUUC A UUUG AAACGUU@LB@ ||||| |||||: || || ||| :|||| |:||||| | |||| |||||:|@LB@ GUUAG UUUGUU UG GU GAG UAAGG GGUAAAG U AAAC UUUGCGA@LB@3'-AUU...GCAAC AACCG AGCA G A AA AAAC UU U AUUAA...CAA-5'@LB@ | |@LB@ 57 38@LB@@LB@@LB@Energy -9.91 kcal/mol@LB@Hybridization Energy -29.8413 kcal/mol@LB@Unfolding Energy - mRNA 5.55001 kcal/mol@LB@Unfolding Energy - sRNA 15.9749 kcal/mol@LB@@LB@Position - mRNA 1 -- 15@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 64 |
0.002007 |
0.1215 |
bsu37680 |
ywfH |
-19.51 |
0.003623 |
4 -- 58 |
32 -- 83 |
bacilysin biosynthesis oxidoreductase YwfH |
|
_____________3___________________________________________________________59\n_____________|___________________________________________________________|\n_________5'-AA___G______U____U__C_____________U__G____AUACAC_______A_____AUAUG...AUU-3'\n______________UUU_GUU_CA_UCUU_GU_GGCAAUC__ACCU_UC_CUUG______UUCCUCA_UUCUU\n______________:||_|||_||_||||_|:_::|||||__|||:_||_|||:______||||:||_|||||\n______________GAA_CAA_GU_AGAA_CG_UUGUUAG__UGGG_AG_GAAU______AAGGGGU_AAGAA\n3'-AUU...UUACC___G___C__U____C__U_______CA____U__A_________________A_____ACUUU...CAA-5'\n_____________|___________________________________________________________|\n_____________84__________________________________________________________31\n |
46 -- 52 |
38 -- 44 |
-36.3278 kcal/mol |
5.00359 kcal/mol |
14.596 kcal/mol |
3 59@LB@ | |@LB@ 5'-AA G U U C U G AUACAC A AUAUG...AUU-3'@LB@ UUU GUU CA UCUU GU GGCAAUC ACCU UC CUUG UUCCUCA UUCUU@LB@ :|| ||| || |||| |: ::||||| |||: || |||: ||||:|| |||||@LB@ GAA CAA GU AGAA CG UUGUUAG UGGG AG GAAU AAGGGGU AAGAA@LB@3'-AUU...UUACC G C U C U CA U A A ACUUU...CAA-5'@LB@ | |@LB@ 84 31@LB@@LB@@LB@Energy -19.51 kcal/mol@LB@Hybridization Energy -36.3278 kcal/mol@LB@Unfolding Energy - mRNA 5.00359 kcal/mol@LB@Unfolding Energy - sRNA 14.596 kcal/mol@LB@@LB@Position - mRNA 4 -- 58@LB@Position - sRNA 32 -- 83@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 123 |
0.005649 |
0.1741 |
bsu37530 |
ywhC |
-10.56 |
0.250674 |
18 -- 29 |
105 -- 116 |
zinc metalloprotease YwhC |
|
_____________17___________30\n_____________|____________|\n5'-GUU...GCUUC____________GACAA...UCG-3'\n______________UGUUACUUUCUC\n______________|||:||||||||\n______________ACAGUGAAAGAG\n3'-AUU...UUACA____________UAGAA...CAA-5'\n_____________|____________|\n_____________117__________104\n |
23 -- 29 |
105 -- 111 |
-16.5612 kcal/mol |
1.98074 kcal/mol |
5.11978 kcal/mol |
17 30@LB@ | |@LB@5'-GUU...GCUUC GACAA...UCG-3'@LB@ UGUUACUUUCUC@LB@ |||:||||||||@LB@ ACAGUGAAAGAG@LB@3'-AUU...UUACA UAGAA...CAA-5'@LB@ | |@LB@ 117 104@LB@@LB@@LB@Energy -10.56 kcal/mol@LB@Hybridization Energy -16.5612 kcal/mol@LB@Unfolding Energy - mRNA 1.98074 kcal/mol@LB@Unfolding Energy - sRNA 5.11978 kcal/mol@LB@@LB@Position - mRNA 18 -- 29@LB@Position - sRNA 105 -- 116@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 28 |
0.0008121 |
0.1059 |
bsu37450 |
ywhK |
-15.95 |
0.020994 |
89 -- 155 |
41 -- 105 |
Evidence 1a: Function experimentally demonstrated in the studied strain; PubMedId: 12073041; Product type f: factor |
bsu37440 |
_____________88_______________________________________156\n_____________|________________________________________|\n5'-UAU...CCUCA_______GUA_______ACA__U_____U____A______GUUAU...UUC-3'\n______________GCAAUUU___CAAGCAA___CG_UACCC_UC_C_UAUUCU\n______________|||||:|___|||:|||___||_|||||_||_|_|||||:\n______________CGUUAGA___GUUUGUU___GC_AUGGG_AG_G_AUAAGG\n3'-AUU...AGCAA_______ACC_______A__________U__A_A______GGUAA...CAA-5'\n_____________|________________________________________|\n_____________106______________________________________40\n |
127 -- 133 |
61 -- 67 |
-28.9674 kcal/mol |
4.84834 kcal/mol |
9.64723 kcal/mol |
88 156@LB@ | |@LB@5'-UAU...CCUCA GUA ACA U U A GUUAU...UUC-3'@LB@ GCAAUUU CAAGCAA CG UACCC UC C UAUUCU@LB@ |||||:| |||:||| || ||||| || | |||||:@LB@ CGUUAGA GUUUGUU GC AUGGG AG G AUAAGG@LB@3'-AUU...AGCAA ACC A U A A GGUAA...CAA-5'@LB@ | |@LB@ 106 40@LB@@LB@@LB@Energy -15.95 kcal/mol@LB@Hybridization Energy -28.9674 kcal/mol@LB@Unfolding Energy - mRNA 4.84834 kcal/mol@LB@Unfolding Energy - sRNA 9.64723 kcal/mol@LB@@LB@Position - mRNA 89 -- 155@LB@Position - sRNA 41 -- 105@LB@Position Seed - mRNA 127 -- 133@LB@Position Seed - sRNA 61 -- 67@LB@ |
