Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
160	0.02589	0.526	b3297	rpsK	-10.33	0.083340	178 -- 193	52 -- 64	30S ribosomal subunit protein S11	_____________177______________194\n_____________\|________________\|\n5'-CGA...AUCAA______AUC_______UUGAA...CUA-3'\n______________GAAAUA___GGGGUGA\n______________\|\|\|\|\|\|___\|\|\|\|\|:\|\n______________CUUUAU___CCCCAUU\n3'-UUU...GCCAA________________UGUCU...UCG-5'\n_____________\|________________\|\n_____________65_______________51\n	187 -- 193	52 -- 58	-13.1856 kcal/mol	2.77978 kcal/mol	0.0778991 kcal/mol	177 194@LB@ \| \|@LB@5'-CGA...AUCAA AUC UUGAA...CUA-3'@LB@ GAAAUA GGGGUGA@LB@ \|\|\|\|\|\| \|\|\|\|\|:\|@LB@ CUUUAU CCCCAUU@LB@3'-UUU...GCCAA UGUCU...UCG-5'@LB@ \| \|@LB@ 65 51@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -13.1856 kcal/mol@LB@Unfolding Energy - mRNA 2.77978 kcal/mol@LB@Unfolding Energy - sRNA 0.0778991 kcal/mol@LB@@LB@Position - mRNA 178 -- 193@LB@Position - sRNA 52 -- 64@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 52 -- 58@LB@
163	0.02649	0.526	b3156	yhbS	-11.68	0.043230	178 -- 217	16 -- 53	GNAT family putative N-acetyltransferase	_____________177_______________________________________218\n_____________\|_________________________________________\|\n5'-CCG...AAACC______________U_______CC___C____UC_______AAUUC...AUC-3'\n______________AAACAGACA_UCGG_AGGUGAA__AUG_UAAU__GAGUAGA\n______________\|\|\|\|\|\|\|\|\|_\|\|:\|_\|::\|\|\|\|__\|:\|_\|\|\|\|__:\|:\|\|:\|\n______________UUUGUCUGU_AGUC_UUUACUU__UGC_AUUA__UUUAUUU\n3'-UUU...CCCCA_________A____________AC___C_____________CAAUU...UCG-5'\n_____________\|_________________________________________\|\n_____________54________________________________________15\n	211 -- 217	16 -- 22	-26.1463 kcal/mol	8.14596 kcal/mol	6.32252 kcal/mol	177 218@LB@ \| \|@LB@5'-CCG...AAACC U CC C UC AAUUC...AUC-3'@LB@ AAACAGACA UCGG AGGUGAA AUG UAAU GAGUAGA@LB@ \|\|\|\|\|\|\|\|\| \|\|:\| \|::\|\|\|\| \|:\| \|\|\|\| :\|:\|\|:\|@LB@ UUUGUCUGU AGUC UUUACUU UGC AUUA UUUAUUU@LB@3'-UUU...CCCCA A AC C CAAUU...UCG-5'@LB@ \| \|@LB@ 54 15@LB@@LB@@LB@Energy -11.68 kcal/mol@LB@Hybridization Energy -26.1463 kcal/mol@LB@Unfolding Energy - mRNA 8.14596 kcal/mol@LB@Unfolding Energy - sRNA 6.32252 kcal/mol@LB@@LB@Position - mRNA 178 -- 217@LB@Position - sRNA 16 -- 53@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 16 -- 22@LB@
189	0.03168	0.5492	b1533	eamA	-9.19	0.139150	178 -- 226	1 -- 49	cysteine and O-acetyl-L-serine efflux system	_____________177_______________________________________________________227\n_____________\|_________________________________________________________\|\n5'-GUU...ACAAA____GAA______________________AAU__CGCGA____A________U____GCUAC...UGA-3'\n______________AGAU___AUUCAGA_______GGCGGUAG___GU_____AAAG_UGG_GGUG_UGGC\n______________\|\|\|:___\|\|\|\|\|\|\|_______\|:\|\|\|\|\|:___:\|_____\|\|\|\|_\|::_\|:\|\|_\|:\|\|\n______________UCUG___UAAGUCU_______CUGCCAUU___UA_____UUUC_AUU_CUAC_AUCG\n3'-UUU...AUUUG______________UUACUUA________AUU___________A___A____U____-5'\n_____________\|________________________________________________________\|\n_____________50_______________________________________________________1\n	193 -- 199	24 -- 30	-25.3484 kcal/mol	9.82981 kcal/mol	6.32371 kcal/mol	177 227@LB@ \| \|@LB@5'-GUU...ACAAA GAA AAU CGCGA A U GCUAC...UGA-3'@LB@ AGAU AUUCAGA GGCGGUAG GU AAAG UGG GGUG UGGC@LB@ \|\|\|: \|\|\|\|\|\|\| \|:\|\|\|\|\|: :\| \|\|\|\| \|:: \|:\|\| \|:\|\|@LB@ UCUG UAAGUCU CUGCCAUU UA UUUC AUU CUAC AUCG@LB@3'-UUU...AUUUG UUACUUA AUU A A U -5'@LB@ \| \|@LB@ 50 1@LB@@LB@@LB@Energy -9.19 kcal/mol@LB@Hybridization Energy -25.3484 kcal/mol@LB@Unfolding Energy - mRNA 9.82981 kcal/mol@LB@Unfolding Energy - sRNA 6.32371 kcal/mol@LB@@LB@Position - mRNA 178 -- 226@LB@Position - sRNA 1 -- 49@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 24 -- 30@LB@
102	0.01618	0.5059	b3306	rpsH	-10.04	0.095208	179 -- 210	1 -- 36	30S ribosomal subunit protein S8	_____________178____________________________________211\n_____________\|______________________________________\|\n5'-AGC...CCAAU_____C________________ACA_______GCAU__AAGAU...AAG-3'\n______________UGAAU_ACGG____GAGUAAAG_____GAUGA____GC\n______________\|\|\|\|\|_\|\|\|\|____:\|:\|\|\|\|\|_____\|\|\|\|\|____\|\|\n______________ACUUA_UGCC____UUUAUUUC_____CUACU____CG\n3'-UUU...UCUUU_____C____AUUA________AAUUA_____AU____-5'\n_____________\|_____________________________________\|\n_____________37____________________________________1\n	190 -- 196	15 -- 21	-20.3749 kcal/mol	4.57112 kcal/mol	5.76239 kcal/mol	178 211@LB@ \| \|@LB@5'-AGC...CCAAU C ACA GCAU AAGAU...AAG-3'@LB@ UGAAU ACGG GAGUAAAG GAUGA GC@LB@ \|\|\|\|\| \|\|\|\| :\|:\|\|\|\|\| \|\|\|\|\| \|\|@LB@ ACUUA UGCC UUUAUUUC CUACU CG@LB@3'-UUU...UCUUU C AUUA AAUUA AU -5'@LB@ \| \|@LB@ 37 1@LB@@LB@@LB@Energy -10.04 kcal/mol@LB@Hybridization Energy -20.3749 kcal/mol@LB@Unfolding Energy - mRNA 4.57112 kcal/mol@LB@Unfolding Energy - sRNA 5.76239 kcal/mol@LB@@LB@Position - mRNA 179 -- 210@LB@Position - sRNA 1 -- 36@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 15 -- 21@LB@
161	0.02632	0.526	b3670	ilvN	-9.27	0.134814	179 -- 196	49 -- 65	acetolactate synthase 1 small subunit	_____________178________________197\n_____________\|__________________\|\n5'-UCA...AAUAC________U___GG____AGCCA...CUU-3'\n______________UGAAAUGG_GGG__AAUA\n______________\|\|\|\|\|\|:\|_\|\|\|__\|\|:\|\n______________ACUUUAUC_CCC__UUGU\n3'-UUU...GGCCA____________AU____CUGUA...UCG-5'\n_____________\|__________________\|\n_____________66_________________48\n	180 -- 186	58 -- 64	-15.3061 kcal/mol	4.40463 kcal/mol	1.63404 kcal/mol	178 197@LB@ \| \|@LB@5'-UCA...AAUAC U GG AGCCA...CUU-3'@LB@ UGAAAUGG GGG AAUA@LB@ \|\|\|\|\|\|:\| \|\|\| \|\|:\|@LB@ ACUUUAUC CCC UUGU@LB@3'-UUU...GGCCA AU CUGUA...UCG-5'@LB@ \| \|@LB@ 66 48@LB@@LB@@LB@Energy -9.27 kcal/mol@LB@Hybridization Energy -15.3061 kcal/mol@LB@Unfolding Energy - mRNA 4.40463 kcal/mol@LB@Unfolding Energy - sRNA 1.63404 kcal/mol@LB@@LB@Position - mRNA 179 -- 196@LB@Position - sRNA 49 -- 65@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 58 -- 64@LB@
194	0.03269	0.5507	b2049	cpsB	-9.94	0.099932	179 -- 235	1 -- 54	mannose-1-phosphate guanyltransferase	_____________178________________________________________________________236\n_____________\|__________________________________________________________\|\n5'-ACA...AUUUA___________________AAUU______CA__CGAAACUCUAUCC___U________AGGUG...GCC-3'\n______________UAAAU_GAUAUUCGGGGAU____AUGGCG__GU_____________AGU_GUGAUGGC\n______________\|\|\|\|:_\|\|:\|\|\|\|:\|::\|\|____\|\|\|:\|\|__\|\|_____________\|:\|_:\|\|\|\|:\|\|\n______________AUUUG_CUGUAAGUCUUUA____UACUGC__CA_____________UUA_UACUAUCG\n3'-UUU...UCCCC_____U_____________CU____________UUAUUUAUUUCAA___C________-5'\n_____________\|_________________________________________________________\|\n_____________55________________________________________________________1\n	229 -- 235	1 -- 7	-30.0746 kcal/mol	13.8011 kcal/mol	6.33758 kcal/mol	178 236@LB@ \| \|@LB@5'-ACA...AUUUA AAUU CA CGAAACUCUAUCC U AGGUG...GCC-3'@LB@ UAAAU GAUAUUCGGGGAU AUGGCG GU AGU GUGAUGGC@LB@ \|\|\|\|: \|\|:\|\|\|\|:\|::\|\| \|\|\|:\|\| \|\| \|:\| :\|\|\|\|:\|\|@LB@ AUUUG CUGUAAGUCUUUA UACUGC CA UUA UACUAUCG@LB@3'-UUU...UCCCC U CU UUAUUUAUUUCAA C -5'@LB@ \| \|@LB@ 55 1@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -30.0746 kcal/mol@LB@Unfolding Energy - mRNA 13.8011 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 179 -- 235@LB@Position - sRNA 1 -- 54@LB@Position Seed - mRNA 229 -- 235@LB@Position Seed - sRNA 1 -- 7@LB@
138	0.02287	0.526	b0081	mraZ	-12.90	0.022954	180 -- 193	52 -- 64	RsmH methytransferase inhibitor	_____________179____________194\n_____________\|______________\|\n5'-GAU...AAGUG_____A________GGCUG...AAA-3'\n______________GGAAU_AGGGGUGA\n______________\|:\|\|\|_\|\|\|\|\|\|:\|\n______________CUUUA_UCCCCAUU\n3'-UUU...GCCAA______________UGUCU...UCG-5'\n_____________\|______________\|\n_____________65_____________51\n	187 -- 193	52 -- 58	-14.7021 kcal/mol	1.72638 kcal/mol	0.0778991 kcal/mol	179 194@LB@ \| \|@LB@5'-GAU...AAGUG A GGCUG...AAA-3'@LB@ GGAAU AGGGGUGA@LB@ \|:\|\|\| \|\|\|\|\|\|:\|@LB@ CUUUA UCCCCAUU@LB@3'-UUU...GCCAA UGUCU...UCG-5'@LB@ \| \|@LB@ 65 51@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -14.7021 kcal/mol@LB@Unfolding Energy - mRNA 1.72638 kcal/mol@LB@Unfolding Energy - sRNA 0.0778991 kcal/mol@LB@@LB@Position - mRNA 180 -- 193@LB@Position - sRNA 52 -- 64@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 52 -- 58@LB@
168	0.02737	0.531	b1385	feaB	-11.89	0.038862	180 -- 210	1 -- 28	phenylacetaldehyde dehydrogenase	_____________179_____________________________211\n_____________\|_______________________________\|\n5'-UUG...CACUG_____AU_______AAA________CAG___CGCAU...GUG-3'\n______________GGUAA__AAUAAGG___AGUGAUGA___AGC\n______________\|\|\|\|\|__\|\|\|\|\|:\|___\|:\|\|\|\|\|\|___\|\|\|\n______________CCAUU__UUAUUUC___UUACUACU___UCG\n3'-UUU...UACUG_____AU_______AA_________A_____-5'\n_____________\|______________________________\|\n_____________29_____________________________1\n	198 -- 204	5 -- 11	-19.4278 kcal/mol	6.77275 kcal/mol	0.766821 kcal/mol	179 211@LB@ \| \|@LB@5'-UUG...CACUG AU AAA CAG CGCAU...GUG-3'@LB@ GGUAA AAUAAGG AGUGAUGA AGC@LB@ \|\|\|\|\| \|\|\|\|\|:\| \|:\|\|\|\|\|\| \|\|\|@LB@ CCAUU UUAUUUC UUACUACU UCG@LB@3'-UUU...UACUG AU AA A -5'@LB@ \| \|@LB@ 29 1@LB@@LB@@LB@Energy -11.89 kcal/mol@LB@Hybridization Energy -19.4278 kcal/mol@LB@Unfolding Energy - mRNA 6.77275 kcal/mol@LB@Unfolding Energy - sRNA 0.766821 kcal/mol@LB@@LB@Position - mRNA 180 -- 210@LB@Position - sRNA 1 -- 28@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 5 -- 11@LB@
10	0.0003219	0.1057	b2727	hypB	-18.91	0.000562	181 -- 230	1 -- 53	GTP hydrolase involved in nickel liganding into hydrogenases	_____________180____________________________________________________231\n_____________\|______________________________________________________\|\n5'-GCC...GAUAG_____________________C____U___CAAC___C_____C______A___AACCU...CGG-3'\n______________AAAUAGAC___CAGGAGUGAG_GAUG_GUA____AUG_GGUUG__GGUGA_GGC\n______________\|\|\|:\|\|\|\|___\|\|\|:\|:\|\|\|:_\|\|:\|_\|\|\|____\|\|:_:\|\|\|:__\|:\|\|\|_:\|\|\n______________UUUGUCUG___GUCUUUACUU_CUGC_CAU____UAU_UCAAU__CUACU_UCG\n3'-UUU...CCCCA________UAA__________A________UAUU___U_____UA_____A___-5'\n_____________\|_____________________________________________________\|\n_____________54____________________________________________________1\n	182 -- 188	46 -- 52	-37.7845 kcal/mol	12.539 kcal/mol	6.33709 kcal/mol	180 231@LB@ \| \|@LB@5'-GCC...GAUAG C U CAAC C C A AACCU...CGG-3'@LB@ AAAUAGAC CAGGAGUGAG GAUG GUA AUG GGUUG GGUGA GGC@LB@ \|\|\|:\|\|\|\| \|\|\|:\|:\|\|\|: \|\|:\| \|\|\| \|\|: :\|\|\|: \|:\|\|\| :\|\|@LB@ UUUGUCUG GUCUUUACUU CUGC CAU UAU UCAAU CUACU UCG@LB@3'-UUU...CCCCA UAA A UAUU U UA A -5'@LB@ \| \|@LB@ 54 1@LB@@LB@@LB@Energy -18.91 kcal/mol@LB@Hybridization Energy -37.7845 kcal/mol@LB@Unfolding Energy - mRNA 12.539 kcal/mol@LB@Unfolding Energy - sRNA 6.33709 kcal/mol@LB@@LB@Position - mRNA 181 -- 230@LB@Position - sRNA 1 -- 53@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 46 -- 52@LB@
57	0.007764	0.4472	b0585	fes	-12.40	0.029926	182 -- 237	1 -- 55	enterobactin/ferrienterobactin esterase	_____________181___________________________________________________________238\n_____________\|_____________________________________________________________\|\n5'-CAU...GCACA_______U__G____________U_______CGU____AGU__GA_____GAGC_______AGUCG...GGU-3'\n______________GUAAACA_GG_GUU_____AUGG_GUGACGG___UAAA___AG__AGUGA____UGGUGGC\n______________\|\|\|\|\|\|\|_\|:_:\|\|_____\|\|\|:_:\|\|\|\|\|\|___\|\|\|\|___\|\|__\|:\|\|\|____\|\|:\|:\|\|\n______________CAUUUGU_CU_UAA_____UACU_UACUGCC___AUUU___UC__UUACU____ACUAUCG\n3'-UUU...AUCCC__________G___GUCUU____________AUU____AUU__AA________________-5'\n_____________\|____________________________________________________________\|\n_____________56___________________________________________________________1\n	231 -- 237	1 -- 7	-34.1879 kcal/mol	15.4551 kcal/mol	6.33758 kcal/mol	181 238@LB@ \| \|@LB@5'-CAU...GCACA U G U CGU AGU GA GAGC AGUCG...GGU-3'@LB@ GUAAACA GG GUU AUGG GUGACGG UAAA AG AGUGA UGGUGGC@LB@ \|\|\|\|\|\|\| \|: :\|\| \|\|\|: :\|\|\|\|\|\| \|\|\|\| \|\| \|:\|\|\| \|\|:\|:\|\|@LB@ CAUUUGU CU UAA UACU UACUGCC AUUU UC UUACU ACUAUCG@LB@3'-UUU...AUCCC G GUCUU AUU AUU AA -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -12.40 kcal/mol@LB@Hybridization Energy -34.1879 kcal/mol@LB@Unfolding Energy - mRNA 15.4551 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 182 -- 237@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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