Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
27	0.001735	0.211	b3052	hldE	-15.91	0.004078	227 -- 261	1 -- 47	heptose 7-phosphate kinase and heptose 1-phosphate adenyltransferase		_____________226______________________________________________262\n_____________\|________________________________________________\|\n5'-GCG...UUUGA____G_____________________________________UGAU__UGGAU...CGC-3'\n______________ACGU_CAG____GAGUGAUGGU_________GGUUGGUGAUG____GC\n______________\|\|:\|_\|\|\|____\|\|:\|\|\|:\|\|\|_________:\|\|\|::\|\|\|\|\|____\|\|\n______________UGUA_GUC____CUUACUGCCA_________UCAAUUACUAC____CG\n3'-UUU...UUGUC____A___UUUA__________UUAUUUAUU___________UAU___-5'\n_____________\|_______________________________________________\|\n_____________48______________________________________________1\n	249 -- 255	6 -- 12	-30.7414 kcal/mol	8.50852 kcal/mol	6.32011 kcal/mol	226 262@LB@ \| \|@LB@5'-GCG...UUUGA G UGAU UGGAU...CGC-3'@LB@ ACGU CAG GAGUGAUGGU GGUUGGUGAUG GC@LB@ \|\|:\| \|\|\| \|\|:\|\|\|:\|\|\| :\|\|\|::\|\|\|\|\| \|\|@LB@ UGUA GUC CUUACUGCCA UCAAUUACUAC CG@LB@3'-UUU...UUGUC A UUUA UUAUUUAUU UAU -5'@LB@ \| \|@LB@ 48 1@LB@@LB@@LB@Energy -15.91 kcal/mol@LB@Hybridization Energy -30.7414 kcal/mol@LB@Unfolding Energy - mRNA 8.50852 kcal/mol@LB@Unfolding Energy - sRNA 6.32011 kcal/mol@LB@@LB@Position - mRNA 227 -- 261@LB@Position - sRNA 1 -- 47@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 6 -- 12@LB@
149	0.02481	0.526	b2108	yehA	-13.80	0.014025	228 -- 241	1 -- 14	putative fimbrial-like adhesin protein		_____________227____________242\n_____________\|______________\|\n5'-GUU...UUAUA______________UAUGC...UUG-3'\n______________UUAAUGAUGAUGGU\n______________\|\|\|\|\|\|\|\|\|\|\|:\|:\n______________AAUUACUACUAUCG\n3'-UUU...AUUUC______________-5'\n_____________\|_____________\|\n_____________15____________1\n	235 -- 241	1 -- 7	-16.5389 kcal/mol	2.69058 kcal/mol	0.0473494 kcal/mol	227 242@LB@ \| \|@LB@5'-GUU...UUAUA UAUGC...UUG-3'@LB@ UUAAUGAUGAUGGU@LB@ \|\|\|\|\|\|\|\|\|\|\|:\|:@LB@ AAUUACUACUAUCG@LB@3'-UUU...AUUUC -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -13.80 kcal/mol@LB@Hybridization Energy -16.5389 kcal/mol@LB@Unfolding Energy - mRNA 2.69058 kcal/mol@LB@Unfolding Energy - sRNA 0.0473494 kcal/mol@LB@@LB@Position - mRNA 228 -- 241@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 1 -- 7@LB@
127	0.01998	0.5155	b2922	yggE	-8.69	0.172995	232 -- 253	1 -- 26	oxidative stress defense protein		_____________231________________________254\n_____________\|__________________________\|\n5'-GCA...GGCAU________________C_________AGCGC...CCG-3'\n______________UAAUGGGUA___UUAG_GG_GAUGGC\n______________\|\|\|\|:::\|\|___\|\|\|:_\|:_\|\|\|:\|\|\n______________AUUAUUUAU___AAUU_CU_CUAUCG\n3'-UUU...CUGCC_________UUC____A__A______-5'\n_____________\|_________________________\|\n_____________27________________________1\n	234 -- 240	18 -- 24	-12.7828 kcal/mol	3.51669 kcal/mol	0.580494 kcal/mol	231 254@LB@ \| \|@LB@5'-GCA...GGCAU C AGCGC...CCG-3'@LB@ UAAUGGGUA UUAG GG GAUGGC@LB@ \|\|\|\|:::\|\| \|\|\|: \|: \|\|\|:\|\|@LB@ AUUAUUUAU AAUU CU CUAUCG@LB@3'-UUU...CUGCC UUC A A -5'@LB@ \| \|@LB@ 27 1@LB@@LB@@LB@Energy -8.69 kcal/mol@LB@Hybridization Energy -12.7828 kcal/mol@LB@Unfolding Energy - mRNA 3.51669 kcal/mol@LB@Unfolding Energy - sRNA 0.580494 kcal/mol@LB@@LB@Position - mRNA 232 -- 253@LB@Position - sRNA 1 -- 26@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 18 -- 24@LB@
120	0.01923	0.5155	b4004	zraR	-8.11	0.219313	234 -- 248	1 -- 12	fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein	b2220	_____________233_____________249\n_____________\|_______________\|\n5'-GGA...UGGUG________CAU____CACUG...CGC-3'\n______________GAUGAUGA___UAGC\n______________:\|\|\|\|\|\|\|___\|\|\|\|\n______________UUACUACU___AUCG\n3'-UUU...UUCAA_______________-5'\n_____________\|______________\|\n_____________13_____________1\n	235 -- 241	5 -- 11	-11.3572 kcal/mol	3.21186 kcal/mol	0.0394463 kcal/mol	233 249@LB@ \| \|@LB@5'-GGA...UGGUG CAU CACUG...CGC-3'@LB@ GAUGAUGA UAGC@LB@ :\|\|\|\|\|\|\| \|\|\|\|@LB@ UUACUACU AUCG@LB@3'-UUU...UUCAA -5'@LB@ \| \|@LB@ 13 1@LB@@LB@@LB@Energy -8.11 kcal/mol@LB@Hybridization Energy -11.3572 kcal/mol@LB@Unfolding Energy - mRNA 3.21186 kcal/mol@LB@Unfolding Energy - sRNA 0.0394463 kcal/mol@LB@@LB@Position - mRNA 234 -- 248@LB@Position - sRNA 1 -- 12@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 5 -- 11@LB@
150	0.02489	0.526	b2088	insE1	-9.25	0.136086	235 -- 276	1 -- 55	IS3 transposase A	b0298 b0373 b0540 b1027	_____________234_____________________________________________________277\n_____________\|_______________________________________________________\|\n5'-ACC...ACCCC_______________CC_______UU__C____________CCC___________UUGCU...CUG-3'\n______________GUAAACAG_CAUUCG____UGAAU__CG______AGUGAAG____UGA____AGC\n______________\|\|\|\|\|\|\|\|_\|\|\|\|\|:____\|\|\|\|\|__\|\|______\|:\|:\|\|\|____\|\|\|____\|\|\|\n______________CAUUUGUC_GUAAGU____ACUUA__GC______UUAUUUC____ACU____UCG\n3'-UUU...AUCCC________U______CUUU_____CU__CAUUAU_______AAUU___ACUA___-5'\n_____________\|______________________________________________________\|\n_____________56_____________________________________________________1\n	261 -- 267	15 -- 21	-23.7484 kcal/mol	8.16492 kcal/mol	6.33758 kcal/mol	234 277@LB@ \| \|@LB@5'-ACC...ACCCC CC UU C CCC UUGCU...CUG-3'@LB@ GUAAACAG CAUUCG UGAAU CG AGUGAAG UGA AGC@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|: \|\|\|\|\| \|\| \|:\|:\|\|\| \|\|\| \|\|\|@LB@ CAUUUGUC GUAAGU ACUUA GC UUAUUUC ACU UCG@LB@3'-UUU...AUCCC U CUUU CU CAUUAU AAUU ACUA -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -9.25 kcal/mol@LB@Hybridization Energy -23.7484 kcal/mol@LB@Unfolding Energy - mRNA 8.16492 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 235 -- 276@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 15 -- 21@LB@
11	0.0003561	0.1063	b3857	mobA	-16.95	0.002119	239 -- 276	15 -- 59	molybdopterin-guanine dinucleotide synthase		_____________238_______________________________________________277\n_____________\|_________________________________________________\|\n5'-UCC...CUGGC___C______GC___________________G_____C___________GAUUG...AAC-3'\n______________AGG_GGUAAA__CAGAC__________GAAU_GGCGG__GUAGAUAAAG\n______________\|\|\|_\|\|\|\|\|\|__\|\|\|\|\|__________\|\|\|\|_\|:\|\|\|__:\|\|:\|\|\|\|\|\|\n______________UCC_CCAUUU__GUCUG__________CUUA_CUGCC__UAUUUAUUUC\n3'-UUU...CUUUA_________________UAAGUCUUUA__________AU__________AAUUA...UCG-5'\n_____________\|_________________________________________________\|\n_____________60________________________________________________14\n	270 -- 276	15 -- 21	-31.2728 kcal/mol	7.99479 kcal/mol	6.32587 kcal/mol	238 277@LB@ \| \|@LB@5'-UCC...CUGGC C GC G C GAUUG...AAC-3'@LB@ AGG GGUAAA CAGAC GAAU GGCGG GUAGAUAAAG@LB@ \|\|\| \|\|\|\|\|\| \|\|\|\|\| \|\|\|\| \|:\|\|\| :\|\|:\|\|\|\|\|\|@LB@ UCC CCAUUU GUCUG CUUA CUGCC UAUUUAUUUC@LB@3'-UUU...CUUUA UAAGUCUUUA AU AAUUA...UCG-5'@LB@ \| \|@LB@ 60 14@LB@@LB@@LB@Energy -16.95 kcal/mol@LB@Hybridization Energy -31.2728 kcal/mol@LB@Unfolding Energy - mRNA 7.99479 kcal/mol@LB@Unfolding Energy - sRNA 6.32587 kcal/mol@LB@@LB@Position - mRNA 239 -- 276@LB@Position - sRNA 15 -- 59@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 15 -- 21@LB@
37	0.00362	0.3212	b4479	dgoR	-15.45	0.005416	239 -- 256	41 -- 57	D-galactonate catabolism operon transcriptional repressor		_____________238________________257\n_____________\|__________________\|\n5'-UUU...ACGCU___________U______CGGCA...AAC-3'\n______________GGGUAAACAGA_CGUUCA\n______________\|\|\|\|\|\|\|\|\|\|\|_\|:\|\|\|\|\n______________CCCAUUUGUCU_GUAAGU\n3'-UUU...UUAUC__________________CUUUA...UCG-5'\n_____________\|__________________\|\n_____________58_________________40\n	243 -- 249	47 -- 53	-24.0794 kcal/mol	2.38777 kcal/mol	6.24592 kcal/mol	238 257@LB@ \| \|@LB@5'-UUU...ACGCU U CGGCA...AAC-3'@LB@ GGGUAAACAGA CGUUCA@LB@ \|\|\|\|\|\|\|\|\|\|\| \|:\|\|\|\|@LB@ CCCAUUUGUCU GUAAGU@LB@3'-UUU...UUAUC CUUUA...UCG-5'@LB@ \| \|@LB@ 58 40@LB@@LB@@LB@Energy -15.45 kcal/mol@LB@Hybridization Energy -24.0794 kcal/mol@LB@Unfolding Energy - mRNA 2.38777 kcal/mol@LB@Unfolding Energy - sRNA 6.24592 kcal/mol@LB@@LB@Position - mRNA 239 -- 256@LB@Position - sRNA 41 -- 57@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 47 -- 53@LB@
121	0.01924	0.5155	b0074	leuA	-11.43	0.049038	243 -- 261	39 -- 56	2-isopropylmalate synthase		_____________242_________________262\n_____________\|___________________\|\n5'-CAU...GCGAC______________A____CAAGC...UGG-3'\n______________GGUGAACAGGCGUU_CAGG\n______________\|\|\|:\|\|\|\|\|:\|:\|\|_\|\|\|:\n______________CCAUUUGUCUGUAA_GUCU\n3'-UUU...UAUCC___________________UUACU...UCG-5'\n_____________\|___________________\|\n_____________57__________________38\n	247 -- 253	46 -- 52	-24.0317 kcal/mol	6.35652 kcal/mol	6.24929 kcal/mol	242 262@LB@ \| \|@LB@5'-CAU...GCGAC A CAAGC...UGG-3'@LB@ GGUGAACAGGCGUU CAGG@LB@ \|\|\|:\|\|\|\|\|:\|:\|\| \|\|\|:@LB@ CCAUUUGUCUGUAA GUCU@LB@3'-UUU...UAUCC UUACU...UCG-5'@LB@ \| \|@LB@ 57 38@LB@@LB@@LB@Energy -11.43 kcal/mol@LB@Hybridization Energy -24.0317 kcal/mol@LB@Unfolding Energy - mRNA 6.35652 kcal/mol@LB@Unfolding Energy - sRNA 6.24929 kcal/mol@LB@@LB@Position - mRNA 243 -- 261@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 46 -- 52@LB@
187	0.03165	0.5492	b2717	hycI	-9.48	0.123006	243 -- 251	1 -- 9	protease involved in processing C-terminal end of HycE		_____________242_______252\n_____________\|_________\|\n5'-AGC...UGGGC_________GCAGG...ACU-3'\n______________GAUGAUGGC\n______________\|\|\|\|\|\|:\|\|\n______________CUACUAUCG\n3'-UUU...AAUUA_________-5'\n_____________\|________\|\n_____________10_______1\n	245 -- 251	1 -- 7	-13.2104 kcal/mol	3.71514 kcal/mol	0.0200155 kcal/mol	242 252@LB@ \| \|@LB@5'-AGC...UGGGC GCAGG...ACU-3'@LB@ GAUGAUGGC@LB@ \|\|\|\|\|\|:\|\|@LB@ CUACUAUCG@LB@3'-UUU...AAUUA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -9.48 kcal/mol@LB@Hybridization Energy -13.2104 kcal/mol@LB@Unfolding Energy - mRNA 3.71514 kcal/mol@LB@Unfolding Energy - sRNA 0.0200155 kcal/mol@LB@@LB@Position - mRNA 243 -- 251@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 1 -- 7@LB@
162	0.02632	0.526	b1886	tar	-10.00	0.096892	244 -- 251	53 -- 60	methyl-accepting chemotaxis protein II		_____________243______252\n_____________\|________\|\n5'-UGC...GGUGC________UUCGC...UUC-3'\n______________UGGGGGUA\n______________\|:\|\|\|\|\|\|\n______________AUCCCCAU\n3'-UUU...ACUUU________UUGUC...UCG-5'\n_____________\|________\|\n_____________61_______52\n	245 -- 251	53 -- 59	-12.2006 kcal/mol	2.12623 kcal/mol	0.0710089 kcal/mol	243 252@LB@ \| \|@LB@5'-UGC...GGUGC UUCGC...UUC-3'@LB@ UGGGGGUA@LB@ \|:\|\|\|\|\|\|@LB@ AUCCCCAU@LB@3'-UUU...ACUUU UUGUC...UCG-5'@LB@ \| \|@LB@ 61 52@LB@@LB@@LB@Energy -10.00 kcal/mol@LB@Hybridization Energy -12.2006 kcal/mol@LB@Unfolding Energy - mRNA 2.12623 kcal/mol@LB@Unfolding Energy - sRNA 0.0710089 kcal/mol@LB@@LB@Position - mRNA 244 -- 251@LB@Position - sRNA 53 -- 60@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 53 -- 59@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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