Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
185	0.03096	0.5492	b1686	menI	-12.09	0.035132	263 -- 300	22 -- 58	1 4-dihydroxy-2-naphthoyl-CoA hydrolase	_____________262____________________________________300\n_____________\|______________________________________\|\n5'-GGC...AUGGU_____UCCU_________CUU_____CAUAU________-3'\n______________GGGGU____GGAUAUUCG____UGAA_____UGGUGAUG\n______________\|\|\|\|\|____:\|\|:\|\|\|\|:____\|\|\|\|_____:\|\|\|:\|\|:\n______________CCCCA____UCUGUAAGU____ACUU_____GCCAUUAU\n3'-UUU...UUUAU_____UUUG_________CUUU____ACU__________UUAUU...UCG-5'\n_____________\|_______________________________________\|\n_____________59______________________________________21\n	294 -- 300	22 -- 28	-26.9755 kcal/mol	8.57608 kcal/mol	6.31389 kcal/mol	262 300@LB@ \| \|@LB@5'-GGC...AUGGU UCCU CUU CAUAU -3'@LB@ GGGGU GGAUAUUCG UGAA UGGUGAUG@LB@ \|\|\|\|\| :\|\|:\|\|\|\|: \|\|\|\| :\|\|\|:\|\|:@LB@ CCCCA UCUGUAAGU ACUU GCCAUUAU@LB@3'-UUU...UUUAU UUUG CUUU ACU UUAUU...UCG-5'@LB@ \| \|@LB@ 59 21@LB@@LB@@LB@Energy -12.09 kcal/mol@LB@Hybridization Energy -26.9755 kcal/mol@LB@Unfolding Energy - mRNA 8.57608 kcal/mol@LB@Unfolding Energy - sRNA 6.31389 kcal/mol@LB@@LB@Position - mRNA 263 -- 300@LB@Position - sRNA 22 -- 58@LB@Position Seed - mRNA 294 -- 300@LB@Position Seed - sRNA 22 -- 28@LB@
151	0.02491	0.526	b3575	yiaK	-7.81	0.245984	13 -- 22	1 -- 10	2 3-diketo-L-gulonate reductase NADH-dependent	_____________12_________23\n_____________\|__________\|\n5'-UUU...CGUCC__________GCAAA...UCG-3'\n______________UGAUGGUAGC\n______________\|\|\|\|\|:\|\|\|\|\n______________ACUACUAUCG\n3'-UUU...CAAUU__________-5'\n_____________\|_________\|\n_____________11________1\n	16 -- 22	1 -- 7	-14.2362 kcal/mol	6.39652 kcal/mol	0.0255427 kcal/mol	12 23@LB@ \| \|@LB@5'-UUU...CGUCC GCAAA...UCG-3'@LB@ UGAUGGUAGC@LB@ \|\|\|\|\|:\|\|\|\|@LB@ ACUACUAUCG@LB@3'-UUU...CAAUU -5'@LB@ \| \|@LB@ 11 1@LB@@LB@@LB@Energy -7.81 kcal/mol@LB@Hybridization Energy -14.2362 kcal/mol@LB@Unfolding Energy - mRNA 6.39652 kcal/mol@LB@Unfolding Energy - sRNA 0.0255427 kcal/mol@LB@@LB@Position - mRNA 13 -- 22@LB@Position - sRNA 1 -- 10@LB@Position Seed - mRNA 16 -- 22@LB@Position Seed - sRNA 1 -- 7@LB@
121	0.01924	0.5155	b0074	leuA	-11.43	0.049038	243 -- 261	39 -- 56	2-isopropylmalate synthase	_____________242_________________262\n_____________\|___________________\|\n5'-CAU...GCGAC______________A____CAAGC...UGG-3'\n______________GGUGAACAGGCGUU_CAGG\n______________\|\|\|:\|\|\|\|\|:\|:\|\|_\|\|\|:\n______________CCAUUUGUCUGUAA_GUCU\n3'-UUU...UAUCC___________________UUACU...UCG-5'\n_____________\|___________________\|\n_____________57__________________38\n	247 -- 253	46 -- 52	-24.0317 kcal/mol	6.35652 kcal/mol	6.24929 kcal/mol	242 262@LB@ \| \|@LB@5'-CAU...GCGAC A CAAGC...UGG-3'@LB@ GGUGAACAGGCGUU CAGG@LB@ \|\|\|:\|\|\|\|\|:\|:\|\| \|\|\|:@LB@ CCAUUUGUCUGUAA GUCU@LB@3'-UUU...UAUCC UUACU...UCG-5'@LB@ \| \|@LB@ 57 38@LB@@LB@@LB@Energy -11.43 kcal/mol@LB@Hybridization Energy -24.0317 kcal/mol@LB@Unfolding Energy - mRNA 6.35652 kcal/mol@LB@Unfolding Energy - sRNA 6.24929 kcal/mol@LB@@LB@Position - mRNA 243 -- 261@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 46 -- 52@LB@
89	0.01433	0.5059	b0636	rlmH	-12.18	0.033475	189 -- 207	1 -- 19	23S rRNA m(3)Psi1915 pseudouridine methyltransferase SAM-dependent	_____________188__________________208\n_____________\|____________________\|\n5'-CGG...AACUC___________C________UGCAA...UCG-3'\n______________UGGAGUUAAUG_GUGA_AGC\n______________\|::\|\|\|\|\|\|\|\|_:\|\|\|_\|\|\|\n______________AUUUCAAUUAC_UACU_UCG\n3'-UUU...UAUUU________________A___-5'\n_____________\|___________________\|\n_____________20__________________1\n	193 -- 199	9 -- 15	-16.4949 kcal/mol	4.25175 kcal/mol	0.0638423 kcal/mol	188 208@LB@ \| \|@LB@5'-CGG...AACUC C UGCAA...UCG-3'@LB@ UGGAGUUAAUG GUGA AGC@LB@ \|::\|\|\|\|\|\|\|\| :\|\|\| \|\|\|@LB@ AUUUCAAUUAC UACU UCG@LB@3'-UUU...UAUUU A -5'@LB@ \| \|@LB@ 20 1@LB@@LB@@LB@Energy -12.18 kcal/mol@LB@Hybridization Energy -16.4949 kcal/mol@LB@Unfolding Energy - mRNA 4.25175 kcal/mol@LB@Unfolding Energy - sRNA 0.0638423 kcal/mol@LB@@LB@Position - mRNA 189 -- 207@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 9 -- 15@LB@
50	0.005695	0.3739	b1086	rluC	-13.13	0.020232	139 -- 168	28 -- 55	23S rRNA pseudouridine(955 2504 2580) synthase	_____________138____________________________169\n_____________\|______________________________\|\n5'-UCC...CAAAA_______UAA____AG_A____________CGAAU...UAC-3'\n______________GUAAAUA___GCAU__A_AAAUGAGUGGCG\n______________\|\|\|\|\|:\|___:\|\|\|__\|_\|\|\|\|\|\|:\|\|:\|\|\n______________CAUUUGU___UGUA__U_UUUACUUACUGC\n3'-UUU...AUCCC_______C______AG_C____________CAUUA...UCG-5'\n_____________\|______________________________\|\n_____________56_____________________________27\n	161 -- 167	29 -- 35	-21.8911 kcal/mol	2.47099 kcal/mol	6.28725 kcal/mol	138 169@LB@ \| \|@LB@5'-UCC...CAAAA UAA AG A CGAAU...UAC-3'@LB@ GUAAAUA GCAU A AAAUGAGUGGCG@LB@ \|\|\|\|\|:\| :\|\|\| \| \|\|\|\|\|\|:\|\|:\|\|@LB@ CAUUUGU UGUA U UUUACUUACUGC@LB@3'-UUU...AUCCC C AG C CAUUA...UCG-5'@LB@ \| \|@LB@ 56 27@LB@@LB@@LB@Energy -13.13 kcal/mol@LB@Hybridization Energy -21.8911 kcal/mol@LB@Unfolding Energy - mRNA 2.47099 kcal/mol@LB@Unfolding Energy - sRNA 6.28725 kcal/mol@LB@@LB@Position - mRNA 139 -- 168@LB@Position - sRNA 28 -- 55@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 29 -- 35@LB@
43	0.004693	0.3568	b1395	paaH	-8.91	0.157263	246 -- 258	49 -- 61	3-hydroxyadipyl-CoA dehydrogenase NAD+-dependent	_____________245___________259\n_____________\|_____________\|\n5'-GGC...GCACC_______C_____UUGCU...UGU-3'\n______________AUGGGGG_AGGCA\n______________\|\|:\|\|\|\|_\|::\|\|\n______________UAUCCCC_UUUGU\n3'-UUU...AACUU_______A_____CUGUA...UCG-5'\n_____________\|_____________\|\n_____________62____________48\n	246 -- 252	55 -- 61	-14.1165 kcal/mol	4.46726 kcal/mol	0.738192 kcal/mol	245 259@LB@ \| \|@LB@5'-GGC...GCACC C UUGCU...UGU-3'@LB@ AUGGGGG AGGCA@LB@ \|\|:\|\|\|\| \|::\|\|@LB@ UAUCCCC UUUGU@LB@3'-UUU...AACUU A CUGUA...UCG-5'@LB@ \| \|@LB@ 62 48@LB@@LB@@LB@Energy -8.91 kcal/mol@LB@Hybridization Energy -14.1165 kcal/mol@LB@Unfolding Energy - mRNA 4.46726 kcal/mol@LB@Unfolding Energy - sRNA 0.738192 kcal/mol@LB@@LB@Position - mRNA 246 -- 258@LB@Position - sRNA 49 -- 61@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 55 -- 61@LB@
160	0.02589	0.526	b3297	rpsK	-10.33	0.083340	178 -- 193	52 -- 64	30S ribosomal subunit protein S11	_____________177______________194\n_____________\|________________\|\n5'-CGA...AUCAA______AUC_______UUGAA...CUA-3'\n______________GAAAUA___GGGGUGA\n______________\|\|\|\|\|\|___\|\|\|\|\|:\|\n______________CUUUAU___CCCCAUU\n3'-UUU...GCCAA________________UGUCU...UCG-5'\n_____________\|________________\|\n_____________65_______________51\n	187 -- 193	52 -- 58	-13.1856 kcal/mol	2.77978 kcal/mol	0.0778991 kcal/mol	177 194@LB@ \| \|@LB@5'-CGA...AUCAA AUC UUGAA...CUA-3'@LB@ GAAAUA GGGGUGA@LB@ \|\|\|\|\|\| \|\|\|\|\|:\|@LB@ CUUUAU CCCCAUU@LB@3'-UUU...GCCAA UGUCU...UCG-5'@LB@ \| \|@LB@ 65 51@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -13.1856 kcal/mol@LB@Unfolding Energy - mRNA 2.77978 kcal/mol@LB@Unfolding Energy - sRNA 0.0778991 kcal/mol@LB@@LB@Position - mRNA 178 -- 193@LB@Position - sRNA 52 -- 64@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 52 -- 58@LB@
102	0.01618	0.5059	b3306	rpsH	-10.04	0.095208	179 -- 210	1 -- 36	30S ribosomal subunit protein S8	_____________178____________________________________211\n_____________\|______________________________________\|\n5'-AGC...CCAAU_____C________________ACA_______GCAU__AAGAU...AAG-3'\n______________UGAAU_ACGG____GAGUAAAG_____GAUGA____GC\n______________\|\|\|\|\|_\|\|\|\|____:\|:\|\|\|\|\|_____\|\|\|\|\|____\|\|\n______________ACUUA_UGCC____UUUAUUUC_____CUACU____CG\n3'-UUU...UCUUU_____C____AUUA________AAUUA_____AU____-5'\n_____________\|_____________________________________\|\n_____________37____________________________________1\n	190 -- 196	15 -- 21	-20.3749 kcal/mol	4.57112 kcal/mol	5.76239 kcal/mol	178 211@LB@ \| \|@LB@5'-AGC...CCAAU C ACA GCAU AAGAU...AAG-3'@LB@ UGAAU ACGG GAGUAAAG GAUGA GC@LB@ \|\|\|\|\| \|\|\|\| :\|:\|\|\|\|\| \|\|\|\|\| \|\|@LB@ ACUUA UGCC UUUAUUUC CUACU CG@LB@3'-UUU...UCUUU C AUUA AAUUA AU -5'@LB@ \| \|@LB@ 37 1@LB@@LB@@LB@Energy -10.04 kcal/mol@LB@Hybridization Energy -20.3749 kcal/mol@LB@Unfolding Energy - mRNA 4.57112 kcal/mol@LB@Unfolding Energy - sRNA 5.76239 kcal/mol@LB@@LB@Position - mRNA 179 -- 210@LB@Position - sRNA 1 -- 36@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 15 -- 21@LB@
113	0.01802	0.5155	b3910	yiiM	-13.90	0.013248	191 -- 239	40 -- 92	6-N-hydroxylaminopurine resistance protein	_____________190______________________________________________________240\n_____________\|________________________________________________________\|\n5'-UAA...GUGGA________A_____________________U_________C_______AGA_____GCUUA...UGC-3'\n______________GAGGGAAC__GAUGCGA__UAUCCGGUUGA_GUA____UA_ACAGGCA___UUCAG\n______________:\|:::\|\|\|__:\|\|\|\|\|\|__:\|\|\|\|\|\|\|\|\|\|_:\|\|____\|\|_\|\|\|\|:\|\|___\|\|\|\|\|\n______________UUUUUUUG__UUACGCU__GUAGGCCAACU_UAU____AU_UGUCUGU___AAGUC\n__________3'-U________GC_______CC___________U___CCCC__U_______________UUUAC...UCG-5'\n_____________\|________________________________________________________\|\n_____________93_______________________________________________________39\n	225 -- 231	45 -- 51	-43.4237 kcal/mol	10.1845 kcal/mol	19.3361 kcal/mol	190 240@LB@ \| \|@LB@5'-UAA...GUGGA A U C AGA GCUUA...UGC-3'@LB@ GAGGGAAC GAUGCGA UAUCCGGUUGA GUA UA ACAGGCA UUCAG@LB@ :\|:::\|\|\| :\|\|\|\|\|\| :\|\|\|\|\|\|\|\|\|\| :\|\| \|\| \|\|\|\|:\|\| \|\|\|\|\|@LB@ UUUUUUUG UUACGCU GUAGGCCAACU UAU AU UGUCUGU AAGUC@LB@ 3'-U GC CC U CCCC U UUUAC...UCG-5'@LB@ \| \|@LB@ 93 39@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -43.4237 kcal/mol@LB@Unfolding Energy - mRNA 10.1845 kcal/mol@LB@Unfolding Energy - sRNA 19.3361 kcal/mol@LB@@LB@Position - mRNA 191 -- 239@LB@Position - sRNA 40 -- 92@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 45 -- 51@LB@
55	0.006796	0.4056	b3192	mlaC	-12.17	0.033659	167 -- 226	1 -- 57	ABC transporter maintaining OM lipid asymmetry periplasmic binding protein	_____________166_______________________________________________________________227\n_____________\|_________________________________________________________________\|\n5'-GGA...CCUGU_____C______AAUAAU____________CC____C_____UU____C_U___________C__UUUGC...AGG-3'\n______________GGGUA_AAC_GA______UUCAGGA__GAA__GACG___AUG__UAAA_G_UUAAUGAUGGU_GC\n______________\|\|\|\|\|_\|\|\|_\|\|______\|\|\|\|\|:\|__\|\|\|__\|\|\|\|___\|\|:__\|\|\|\|_\|_\|\|\|\|\|\|\|\|\|:\|_\|\|\n______________CCCAU_UUG_CU______AAGUCUU__CUU__CUGC___UAU__AUUU_C_AAUUACUACUA_CG\n3'-UUU...UUAUC_________U__GU___________UA___A_____CAU___UU__________________U__-5'\n_____________\|________________________________________________________________\|\n_____________58_______________________________________________________________1\n	217 -- 223	4 -- 10	-32.157 kcal/mol	13.6506 kcal/mol	6.33758 kcal/mol	166 227@LB@ \| \|@LB@5'-GGA...CCUGU C AAUAAU CC C UU C U C UUUGC...AGG-3'@LB@ GGGUA AAC GA UUCAGGA GAA GACG AUG UAAA G UUAAUGAUGGU GC@LB@ \|\|\|\|\| \|\|\| \|\| \|\|\|\|\|:\| \|\|\| \|\|\|\| \|\|: \|\|\|\| \| \|\|\|\|\|\|\|\|\|:\| \|\|@LB@ CCCAU UUG CU AAGUCUU CUU CUGC UAU AUUU C AAUUACUACUA CG@LB@3'-UUU...UUAUC U GU UA A CAU UU U -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -12.17 kcal/mol@LB@Hybridization Energy -32.157 kcal/mol@LB@Unfolding Energy - mRNA 13.6506 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 167 -- 226@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 4 -- 10@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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