| 50 |
0.001627 |
0.1212 |
bsu38130 |
ywcE |
-9.91 |
0.324646 |
1 -- 15 |
39 -- 56 |
spore morphogenesis and germination protein YwcE |
|
_____________0_______________________________________________________________16\n_____________|_______________________________________________________________|\n5'-GCC...AAAAA_____ACA______________A__A___A______U____________UU____U_______AUCAC...CAA-3'\n______________CAAUC_____AAACAG____AC_CA_CUC__GUUCC_CUAUUUC____A__UUUG_AAACGUU\n______________|||||_____|||||:____||_||_|||__:||||_|:|||||____|__||||_|||||:|\n______________GUUAG_____UUUGUU____UG_GU_GAG__UAAGG_GGUAAAG____U__AAAC_UUUGCGA\n3'-AUU...GCAAC_____AACCG______AGCA__G__A___AA_____________AAAC_UU____U_______AUUAA...CAA-5'\n_____________|_______________________________________________________________|\n_____________57______________________________________________________________38\n |
170 -- 176 |
15 -- 21 |
-29.8413 kcal/mol |
5.55001 kcal/mol |
15.9749 kcal/mol |
0 16@LB@ | |@LB@5'-GCC...AAAAA ACA A A A U UU U AUCAC...CAA-3'@LB@ CAAUC AAACAG AC CA CUC GUUCC CUAUUUC A UUUG AAACGUU@LB@ ||||| |||||: || || ||| :|||| |:||||| | |||| |||||:|@LB@ GUUAG UUUGUU UG GU GAG UAAGG GGUAAAG U AAAC UUUGCGA@LB@3'-AUU...GCAAC AACCG AGCA G A AA AAAC UU U AUUAA...CAA-5'@LB@ | |@LB@ 57 38@LB@@LB@@LB@Energy -9.91 kcal/mol@LB@Hybridization Energy -29.8413 kcal/mol@LB@Unfolding Energy - mRNA 5.55001 kcal/mol@LB@Unfolding Energy - sRNA 15.9749 kcal/mol@LB@@LB@Position - mRNA 1 -- 15@LB@Position - sRNA 39 -- 56@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 51 |
0.001644 |
0.1212 |
bsu29870 |
ytzB |
-11.72 |
0.152375 |
23 -- 59 |
33 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________22__________________________________________60\n_____________|___________________________________________|\n5'-CUG...AUUGC___________________________________________AAAAG...AAG-3'\n______________UUAACAGGAU_UUGGC__UGUU__________UCUGUAUAAUG\n______________|:|||||||:_|||:|__|||:__________||::|||||||\n______________AGUUGUCCUG_AACUG__ACAG__________AGGUAUAUUAC\n3'-AUU...GAAGU__________U_____UA____GCGUCGUUCA___________AACAG...CAA-5'\n_____________|___________________________________________|\n_____________86__________________________________________32\n |
163 -- 169 |
118 -- 124 |
-27.3559 kcal/mol |
7.06665 kcal/mol |
10.6143 kcal/mol |
22 60@LB@ | |@LB@5'-CUG...AUUGC AAAAG...AAG-3'@LB@ UUAACAGGAU UUGGC UGUU UCUGUAUAAUG@LB@ |:|||||||: |||:| |||: ||::|||||||@LB@ AGUUGUCCUG AACUG ACAG AGGUAUAUUAC@LB@3'-AUU...GAAGU U UA GCGUCGUUCA AACAG...CAA-5'@LB@ | |@LB@ 86 32@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -27.3559 kcal/mol@LB@Unfolding Energy - mRNA 7.06665 kcal/mol@LB@Unfolding Energy - sRNA 10.6143 kcal/mol@LB@@LB@Position - mRNA 23 -- 59@LB@Position - sRNA 33 -- 85@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 52 |
0.001732 |
0.1212 |
bsu29530 |
sppA |
-10.15 |
0.295539 |
2 -- 29 |
39 -- 81 |
signal peptide peptidase SppA |
|
_____________1________________________________________________________30\n_____________|________________________________________________________|\n5'-GCG...CUCUG_G______C____UGUCUAUU_____CAG__________GA_______________GCGUC...AAG-3'\n______________G_GAUUUU_GGCG________AUCGU___CAUCUC_UAU_____GUUUCUUUGAAA\n______________|_:||:||_|||:________|||||___||||||_|||_____:|||||||||||\n______________C_UUAGAA_CCGU________UAGCA___GUAGAG_AUA_____UAAAGAAACUUU\n3'-AUU...AGCAA_G___________UUGU_________UGG______A___AGGGG____________AAACU...CAA-5'\n_____________|________________________________________________________|\n_____________82_______________________________________________________38\n |
284 -- 290 |
28 -- 34 |
-34.8894 kcal/mol |
12.7856 kcal/mol |
14.4529 kcal/mol |
1 30@LB@ | |@LB@5'-GCG...CUCUG G C UGUCUAUU CAG GA GCGUC...AAG-3'@LB@ G GAUUUU GGCG AUCGU CAUCUC UAU GUUUCUUUGAAA@LB@ | :||:|| |||: ||||| |||||| ||| :|||||||||||@LB@ C UUAGAA CCGU UAGCA GUAGAG AUA UAAAGAAACUUU@LB@3'-AUU...AGCAA G UUGU UGG A AGGGG AAACU...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -10.15 kcal/mol@LB@Hybridization Energy -34.8894 kcal/mol@LB@Unfolding Energy - mRNA 12.7856 kcal/mol@LB@Unfolding Energy - sRNA 14.4529 kcal/mol@LB@@LB@Position - mRNA 2 -- 29@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 53 |
0.001762 |
0.1212 |
bsu10040 |
ecsA |
-7.56 |
0.695140 |
42 -- 51 |
33 -- 42 |
ABC transporter ATP-binding protein |
|
_____________41_________52\n_____________|__________|\n5'-UAU...AAACA__________CAGUU...GCU-3'\n______________CCUCAUUUUU\n______________||:|||||:|\n______________GGGGUAAAGA\n3'-AUU...AAUAA__________AACUU...CAA-5'\n_____________|__________|\n_____________43_________32\n |
45 -- 51 |
33 -- 39 |
-10.6739 kcal/mol |
0.405129 kcal/mol |
4.65815 kcal/mol |
41 52@LB@ | |@LB@5'-UAU...AAACA CAGUU...GCU-3'@LB@ CCUCAUUUUU@LB@ ||:|||||:|@LB@ GGGGUAAAGA@LB@3'-AUU...AAUAA AACUU...CAA-5'@LB@ | |@LB@ 43 32@LB@@LB@@LB@Energy -7.56 kcal/mol@LB@Hybridization Energy -10.6739 kcal/mol@LB@Unfolding Energy - mRNA 0.405129 kcal/mol@LB@Unfolding Energy - sRNA 4.65815 kcal/mol@LB@@LB@Position - mRNA 42 -- 51@LB@Position - sRNA 33 -- 42@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 54 |
0.001793 |
0.1212 |
bsu21030 |
yonP |
-11.11 |
0.198660 |
20 -- 36 |
161 -- 178 |
Evidence 5: No homology to any previously reported sequences |
|
_____________19_______37\n_____________|________|\n5'-AAU...UUUAA________AUAUU...ACA-3'\n______________ACUCUUUA\n______________||||||:|\n______________UGAGAAGU\n3'-AUU...AAAAA________AGUUG...CAA-5'\n_____________|________|\n_____________179______160\n |
30 -- 36 |
161 -- 167 |
-8.6372 kcal/mol |
0.252156 kcal/mol |
0.576912 kcal/mol |
19 37@LB@ | |@LB@5'-AAU...UUUAA AUAUU...ACA-3'@LB@ ACUCUUUA@LB@ ||||||:|@LB@ UGAGAAGU@LB@3'-AUU...AAAAA AGUUG...CAA-5'@LB@ | |@LB@ 179 160@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -8.6372 kcal/mol@LB@Unfolding Energy - mRNA 0.252156 kcal/mol@LB@Unfolding Energy - sRNA 0.576912 kcal/mol@LB@@LB@Position - mRNA 20 -- 36@LB@Position - sRNA 161 -- 178@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 161 -- 167@LB@ |
| 55 |
0.001813 |
0.1212 |
bsu30290 |
amyC |
-12.90 |
0.089506 |
91 -- 153 |
102 -- 159 |
ABC transporter permease |
|
_____________90_________________________________________________________________154\n_____________|__________________________________________________________________|\n5'-CUG...UCAAA_________GCC___UA________AC_____G_______UU___GCAU__________GA_____AGCUU...CUU-3'\n______________CGGCAGGAU___GGC__UG_CCGCA__GCAAG___CAUAU__AUG____UUGUCAUUUU__UCAUC\n______________|::|||||:___|:|__||_|||||__|||||___|||||__|||____||||||:|||__|||||\n______________GUUGUCCUG___CUG__AC_GGCGU__CGUUC___GUAUA__UAC____AACAGUGAAA__AGUAG\n3'-AUU...AAGUA_________UAA___UA__A____________AAG_____U__________________G______AAGUU...CAA-5'\n_____________|__________________________________________________________________|\n_____________160________________________________________________________________101\n |
139 -- 145 |
109 -- 115 |
-48.9264 kcal/mol |
23.4207 kcal/mol |
14.7573 kcal/mol |
90 154@LB@ | |@LB@5'-CUG...UCAAA GCC UA AC G UU GCAU GA AGCUU...CUU-3'@LB@ CGGCAGGAU GGC UG CCGCA GCAAG CAUAU AUG UUGUCAUUUU UCAUC@LB@ |::|||||: |:| || ||||| ||||| ||||| ||| ||||||:||| |||||@LB@ GUUGUCCUG CUG AC GGCGU CGUUC GUAUA UAC AACAGUGAAA AGUAG@LB@3'-AUU...AAGUA UAA UA A AAG U G AAGUU...CAA-5'@LB@ | |@LB@ 160 101@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -48.9264 kcal/mol@LB@Unfolding Energy - mRNA 23.4207 kcal/mol@LB@Unfolding Energy - sRNA 14.7573 kcal/mol@LB@@LB@Position - mRNA 91 -- 153@LB@Position - sRNA 102 -- 159@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 109 -- 115@LB@ |
| 56 |
0.00183 |
0.1212 |
bsu11770 |
cotW |
-16.10 |
0.019548 |
48 -- 170 |
31 -- 163 |
spore coat protein W |
|
_____________47_______________________________________171\n_____________|________________________________________|\n5'-UCU...UAAAA__________CC__________CAAAAUUACUU_______CGACG...GGG-3'\n______________UUAUCAACAG__GCA___ACAU___________UGUCUGC\n______________|:||||||||__:||___||||___________||||:||\n______________AGUAGUUGUC__UGU___UGUA___________ACAGGCG\n3'-AUU...UGAGA__________C____AAC______________________UCGUU...CAA-5'\n_____________|________________________________________|\n_____________164______________________________________30\n |
78 -- 84 |
136 -- 142 |
-21.9263 kcal/mol |
4.68907 kcal/mol |
7.4131 kcal/mol |
47 171@LB@ | |@LB@5'-UCU...UAAAA CC CAAAAUUACUU CGACG...GGG-3'@LB@ UUAUCAACAG GCA ACAU UGUCUGC@LB@ |:|||||||| :|| |||| ||||:||@LB@ AGUAGUUGUC UGU UGUA ACAGGCG@LB@3'-AUU...UGAGA C AAC UCGUU...CAA-5'@LB@ | |@LB@ 164 30@LB@@LB@@LB@Energy -16.10 kcal/mol@LB@Hybridization Energy -21.9263 kcal/mol@LB@Unfolding Energy - mRNA 4.68907 kcal/mol@LB@Unfolding Energy - sRNA 7.4131 kcal/mol@LB@@LB@Position - mRNA 48 -- 170@LB@Position - sRNA 31 -- 163@LB@Position Seed - mRNA 78 -- 84@LB@Position Seed - sRNA 136 -- 142@LB@ |
| 57 |
0.001839 |
0.1212 |
bsu14940 |
ylbA |
-10.08 |
0.303417 |
14 -- 67 |
35 -- 81 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________13___________________________________________68\n_____________|____________________________________________|\n5'-CAU...AAUAC_________AUU______U___C___C___AG____________UGUCA...AAU-3'\n______________CG_UGCAAU____GCAAG_CGG_UGU_AUC__AUCUUUUAUUUU\n______________||_||||||____||||:_|::_:||_|:|__||||:|||||::\n______________GC_ACGUUA____CGUUU_GUU_GCA_UGG__UAGAGAAUAAGG\n3'-AUU...CCGAA__A______GAAC_________A_______G_____________GGUAA...CAA-5'\n_____________|____________________________________________|\n_____________82___________________________________________34\n |
49 -- 55 |
41 -- 47 |
-26.0695 kcal/mol |
9.01529 kcal/mol |
9.64997 kcal/mol |
13 68@LB@ | |@LB@5'-CAU...AAUAC AUU U C C AG UGUCA...AAU-3'@LB@ CG UGCAAU GCAAG CGG UGU AUC AUCUUUUAUUUU@LB@ || |||||| ||||: |:: :|| |:| ||||:|||||::@LB@ GC ACGUUA CGUUU GUU GCA UGG UAGAGAAUAAGG@LB@3'-AUU...CCGAA A GAAC A G GGUAA...CAA-5'@LB@ | |@LB@ 82 34@LB@@LB@@LB@Energy -10.08 kcal/mol@LB@Hybridization Energy -26.0695 kcal/mol@LB@Unfolding Energy - mRNA 9.01529 kcal/mol@LB@Unfolding Energy - sRNA 9.64997 kcal/mol@LB@@LB@Position - mRNA 14 -- 67@LB@Position - sRNA 35 -- 81@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 41 -- 47@LB@ |
| 58 |
0.001848 |
0.1212 |
bsu21840 |
ypjP |
-10.31 |
0.277541 |
15 -- 73 |
14 -- 70 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________14___________________________________________________________74\n_____________|____________________________________________________________|\n5'-UAA...AACUG__________AG___CAA____GCGGAAAG____C____A_______A___U________UUACA...CCG-3'\n______________UGGCAAACAG__CGU___UCAU________CCUC_UUUC__UGAAAU_UUG_AAGCGUUU\n______________|||||||||:__|||___:|||________||:|_||||__||||||_|||_||:||:||\n______________ACCGUUUGUU__GCA___GGUA________GGGG_AAAG__ACUUUA_AAC_UUUGCGAA\n3'-AUU...UUAGA__________A____UG_____GAGAAUAA____U____AA__________U________UUAAA...CAA-5'\n_____________|____________________________________________________________|\n_____________71___________________________________________________________13\n |
67 -- 73 |
14 -- 20 |
-38.3558 kcal/mol |
13.3253 kcal/mol |
15.5628 kcal/mol |
14 74@LB@ | |@LB@5'-UAA...AACUG AG CAA GCGGAAAG C A A U UUACA...CCG-3'@LB@ UGGCAAACAG CGU UCAU CCUC UUUC UGAAAU UUG AAGCGUUU@LB@ |||||||||: ||| :||| ||:| |||| |||||| ||| ||:||:||@LB@ ACCGUUUGUU GCA GGUA GGGG AAAG ACUUUA AAC UUUGCGAA@LB@3'-AUU...UUAGA A UG GAGAAUAA U AA U UUAAA...CAA-5'@LB@ | |@LB@ 71 13@LB@@LB@@LB@Energy -10.31 kcal/mol@LB@Hybridization Energy -38.3558 kcal/mol@LB@Unfolding Energy - mRNA 13.3253 kcal/mol@LB@Unfolding Energy - sRNA 15.5628 kcal/mol@LB@@LB@Position - mRNA 15 -- 73@LB@Position - sRNA 14 -- 70@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 14 -- 20@LB@ |
| 59 |
0.001868 |
0.1212 |
bsu12260 |
yjlA |
-14.41 |
0.044077 |
46 -- 133 |
103 -- 182 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pt: transporter' |
|
_____________45__________________________________________________________________134\n_____________|___________________________________________________________________|\n5'-CGG...UGUCU_____UC_______UUCACCUCCCUUUU______UCCACAUAUA_____UCG_____AAC_______UUUUC...GGA-3'\n______________CUCUU__CAUCAAU______________UUGUCU__________GCGGU_____CCA___AUGAUGU\n______________|||||__||||||:______________|||||:__________||:|:_____|||___||:||||\n______________GAGAA__GUAGUUG______________AACAGG__________CGUCG_____GGU___UAUUACA\n3'-AUU...AAAAU______________UCCUGUAACUGU_______________________UUCAA___A_________ACAGU...CAA-5'\n_____________|___________________________________________________________________|\n_____________183_________________________________________________________________102\n |
118 -- 124 |
117 -- 123 |
-27.4081 kcal/mol |
7.08838 kcal/mol |
10.6441 kcal/mol |
45 134@LB@ | |@LB@5'-CGG...UGUCU UC UUCACCUCCCUUUU UCCACAUAUA UCG AAC UUUUC...GGA-3'@LB@ CUCUU CAUCAAU UUGUCU GCGGU CCA AUGAUGU@LB@ ||||| ||||||: |||||: ||:|: ||| ||:||||@LB@ GAGAA GUAGUUG AACAGG CGUCG GGU UAUUACA@LB@3'-AUU...AAAAU UCCUGUAACUGU UUCAA A ACAGU...CAA-5'@LB@ | |@LB@ 183 102@LB@@LB@@LB@Energy -14.41 kcal/mol@LB@Hybridization Energy -27.4081 kcal/mol@LB@Unfolding Energy - mRNA 7.08838 kcal/mol@LB@Unfolding Energy - sRNA 10.6441 kcal/mol@LB@@LB@Position - mRNA 46 -- 133@LB@Position - sRNA 103 -- 182@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 117 -- 123@LB@ |
