| 125 |
0.005817 |
0.1741 |
bsu26980 |
yraE |
-11.00 |
0.208718 |
35 -- 90 |
112 -- 185 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________34________________________________________________________91\n_____________|_________________________________________________________|\n5'-UAC...UUUGA___AG______________AA_______A____GGA_____________________AGCCA...CUG-3'\n______________ACU___UAUCGACAGG_CA_____AUUG_UCCG_______________GAUGUUGUC\n______________|||___:|||:|||||_||_____||||_||||_______________:||||||||\n______________UGA___GUAGUUGUCC_GU_____UAAC_AGGC_______________UUACAACAG\n3'-AUU...AAAAA___GAA__________U__AACUG_________GUCGUUCAAGGUAUA_________UGAAA...CAA-5'\n_____________|_________________________________________________________|\n_____________186_______________________________________________________111\n |
83 -- 89 |
113 -- 119 |
-30.5681 kcal/mol |
8.54797 kcal/mol |
10.8348 kcal/mol |
34 91@LB@ | |@LB@5'-UAC...UUUGA AG AA A GGA AGCCA...CUG-3'@LB@ ACU UAUCGACAGG CA AUUG UCCG GAUGUUGUC@LB@ ||| :|||:||||| || |||| |||| :||||||||@LB@ UGA GUAGUUGUCC GU UAAC AGGC UUACAACAG@LB@3'-AUU...AAAAA GAA U AACUG GUCGUUCAAGGUAUA UGAAA...CAA-5'@LB@ | |@LB@ 186 111@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -30.5681 kcal/mol@LB@Unfolding Energy - mRNA 8.54797 kcal/mol@LB@Unfolding Energy - sRNA 10.8348 kcal/mol@LB@@LB@Position - mRNA 35 -- 90@LB@Position - sRNA 112 -- 185@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 113 -- 119@LB@ |
| 126 |
0.005872 |
0.1741 |
bsu36510 |
amtB |
-10.53 |
0.253946 |
51 -- 89 |
33 -- 57 |
ammonium transporter NrgA |
|
_____________50_____________________________________________________________________________________90\n_____________|______________________________________________________________________________________|\n______5'-CUCAU______A_____UUUUAUAGAUACAA______AUAUC____AAAA____AU____C_______UUCCAUCCCUCGAUAA_______CAAAA...GCA-3'\n______________AUGAAU_GUUUC______________AGUCUU_____GCAA____UGCC__UCUC___UUCCU________________CAUUUCU\n______________||||||_|:|||______________|:||||_____:|||____|:||__||||___||||:________________|||||||\n______________UACUUA_CGAAG______________UUAGAA_____UGUU____AUGG__AGAG___AAGGG________________GUAAAGA\n3'-AUU...UUCAU______C_____CAACG_______________CCGUU____AGC_____GU____AAU____________________________AACUU...CAA-5'\n_____________|______________________________________________________________________________________|\n_____________58_____________________________________________________________________________________32\n |
83 -- 89 |
33 -- 39 |
-30.7855 kcal/mol |
7.29289 kcal/mol |
15.7787 kcal/mol |
50 90@LB@ | |@LB@ 5'-CUCAU A UUUUAUAGAUACAA AUAUC AAAA AU C UUCCAUCCCUCGAUAA CAAAA...GCA-3'@LB@ AUGAAU GUUUC AGUCUU GCAA UGCC UCUC UUCCU CAUUUCU@LB@ |||||| |:||| |:|||| :||| |:|| |||| ||||: |||||||@LB@ UACUUA CGAAG UUAGAA UGUU AUGG AGAG AAGGG GUAAAGA@LB@3'-AUU...UUCAU C CAACG CCGUU AGC GU AAU AACUU...CAA-5'@LB@ | |@LB@ 58 32@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -30.7855 kcal/mol@LB@Unfolding Energy - mRNA 7.29289 kcal/mol@LB@Unfolding Energy - sRNA 15.7787 kcal/mol@LB@@LB@Position - mRNA 51 -- 89@LB@Position - sRNA 33 -- 57@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 127 |
0.005895 |
0.1741 |
bsu15640 |
yloA |
-14.75 |
0.037563 |
39 -- 86 |
36 -- 83 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 11238985 17586671; Product type pf: factor' |
|
_____________38__________________________________________________87\n_____________|___________________________________________________|\n5'-UUA...ACGUA__G_____C____A_______CUGU_____________AUGAAA_______CCGCU...AUA-3'\n______________UU_GUUGC__UCU__GUAAAU____UUGU_CCCAUUUC______CUUCAUU\n______________||_|||||__|||__|:|||:____|:||_|||||:||______|::||||\n______________AA_CAACG__AGA__CGUUUG____AGCA_GGGUAGAG______GGGGUAA\n3'-AUU...UACCG__G_____UU___AC______UU______U________AAUAA________AGAAA...CAA-5'\n_____________|___________________________________________________|\n_____________84__________________________________________________35\n |
80 -- 86 |
36 -- 42 |
-30.597 kcal/mol |
5.41607 kcal/mol |
13.7079 kcal/mol |
38 87@LB@ | |@LB@5'-UUA...ACGUA G C A CUGU AUGAAA CCGCU...AUA-3'@LB@ UU GUUGC UCU GUAAAU UUGU CCCAUUUC CUUCAUU@LB@ || ||||| ||| |:|||: |:|| |||||:|| |::||||@LB@ AA CAACG AGA CGUUUG AGCA GGGUAGAG GGGGUAA@LB@3'-AUU...UACCG G UU AC UU U AAUAA AGAAA...CAA-5'@LB@ | |@LB@ 84 35@LB@@LB@@LB@Energy -14.75 kcal/mol@LB@Hybridization Energy -30.597 kcal/mol@LB@Unfolding Energy - mRNA 5.41607 kcal/mol@LB@Unfolding Energy - sRNA 13.7079 kcal/mol@LB@@LB@Position - mRNA 39 -- 86@LB@Position - sRNA 36 -- 83@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 128 |
0.005903 |
0.1741 |
bsu24110 |
yqzF |
-12.53 |
0.106013 |
93 -- 138 |
39 -- 81 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________92_______________________________________________139\n_____________|________________________________________________|\n5'-UAA...UAUUC_________AAUUU___C___CCUA___UA___CGUUUUCU_______ACAAA...UCA-3'\n______________CGUUGCAAU_____GCA_AAC____UGU__CCA________UCUCUUG\n______________|||||||||_____|||_|||____:||__|||________||||||:\n______________GCAACGUUA_____CGU_UUG____GCA__GGU________AGAGAAU\n3'-AUU...CCGAA_________GAAC________UUA____UG__________________AAGGG...CAA-5'\n_____________|________________________________________________|\n_____________82_______________________________________________38\n |
134 -- 140 |
45 -- 51 |
-26.5343 kcal/mol |
8.17713 kcal/mol |
8.56905 kcal/mol |
92 139@LB@ | |@LB@5'-UAA...UAUUC AAUUU C CCUA UA CGUUUUCU ACAAA...UCA-3'@LB@ CGUUGCAAU GCA AAC UGU CCA UCUCUUG@LB@ ||||||||| ||| ||| :|| ||| ||||||:@LB@ GCAACGUUA CGU UUG GCA GGU AGAGAAU@LB@3'-AUU...CCGAA GAAC UUA UG AAGGG...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -26.5343 kcal/mol@LB@Unfolding Energy - mRNA 8.17713 kcal/mol@LB@Unfolding Energy - sRNA 8.56905 kcal/mol@LB@@LB@Position - mRNA 93 -- 138@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 129 |
0.005948 |
0.1741 |
bsu13580 |
ykrV |
-12.37 |
0.114104 |
93 -- 132 |
31 -- 61 |
transaminase MtnE |
|
_____________92________________________________________133\n_____________|_________________________________________|\n5'-CAG...AAGAU________AUUU________UAACA_____AAUU_______ACUUU...ACG-3'\n______________AUCGU_CC____CAUCUUUU_____CCCCA____UUUCUUU\n______________|||||_||____|||||:||_____|||||____|||||||\n______________UAGCA_GG____GUAGAGAA_____GGGGU____AAAGAAA\n3'-AUU...UUUGU_____U______________UAA__________________CUUUA...CAA-5'\n_____________|_________________________________________|\n_____________62________________________________________30\n |
126 -- 132 |
31 -- 37 |
-24.5638 kcal/mol |
3.82436 kcal/mol |
10.4555 kcal/mol |
92 133@LB@ | |@LB@5'-CAG...AAGAU AUUU UAACA AAUU ACUUU...ACG-3'@LB@ AUCGU CC CAUCUUUU CCCCA UUUCUUU@LB@ ||||| || |||||:|| ||||| |||||||@LB@ UAGCA GG GUAGAGAA GGGGU AAAGAAA@LB@3'-AUU...UUUGU U UAA CUUUA...CAA-5'@LB@ | |@LB@ 62 30@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -24.5638 kcal/mol@LB@Unfolding Energy - mRNA 3.82436 kcal/mol@LB@Unfolding Energy - sRNA 10.4555 kcal/mol@LB@@LB@Position - mRNA 93 -- 132@LB@Position - sRNA 31 -- 61@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 130 |
0.005995 |
0.1741 |
bsu19660 |
yozD |
-10.24 |
0.285413 |
18 -- 51 |
105 -- 135 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________17_________________________________52\n_____________|__________________________________|\n5'-CAC...CUUUG____UUUU_____U______AUCAAAU_______CGUCC...UGU-3'\n______________AGCG____UUCCA_UAUGAU_______CUUUCUC\n______________|||:____|||||_|||:||_______|||||||\n______________UCGU____AAGGU_AUAUUA_______GAAAGAG\n3'-AUU...AGGCG____UC______________CAACAGU_______UAGAA...CAA-5'\n_____________|__________________________________|\n_____________136________________________________104\n |
45 -- 51 |
105 -- 111 |
-19.2876 kcal/mol |
2.67508 kcal/mol |
6.61557 kcal/mol |
17 52@LB@ | |@LB@5'-CAC...CUUUG UUUU U AUCAAAU CGUCC...UGU-3'@LB@ AGCG UUCCA UAUGAU CUUUCUC@LB@ |||: ||||| |||:|| |||||||@LB@ UCGU AAGGU AUAUUA GAAAGAG@LB@3'-AUU...AGGCG UC CAACAGU UAGAA...CAA-5'@LB@ | |@LB@ 136 104@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -19.2876 kcal/mol@LB@Unfolding Energy - mRNA 2.67508 kcal/mol@LB@Unfolding Energy - sRNA 6.61557 kcal/mol@LB@@LB@Position - mRNA 18 -- 51@LB@Position - sRNA 105 -- 135@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 131 |
0.006003 |
0.1741 |
bsu37510 |
pbpG |
-10.90 |
0.216982 |
70 -- 106 |
84 -- 108 |
penicillin-binding protein 2D |
|
_____________69____________________________________107\n_____________|_____________________________________|\n5'-UGU...UGAAA___________CCUAAGGAAA_____UAGA_______GGGGA...UGU-3'\n______________UCUCAUUUUCA__________AAGUA____GGAUGGC\n______________||||||:||||__________|||||____|:|||||\n______________AGAGUAGAAGU__________UUCAU____CUUACCG\n3'-AUU...GUGAA__________________________UA_________AAGCA...CAA-5'\n_____________|_____________________________________|\n_____________109___________________________________83\n |
100 -- 106 |
84 -- 90 |
-24.2284 kcal/mol |
7.40636 kcal/mol |
9.09036 kcal/mol |
69 107@LB@ | |@LB@5'-UGU...UGAAA CCUAAGGAAA UAGA GGGGA...UGU-3'@LB@ UCUCAUUUUCA AAGUA GGAUGGC@LB@ ||||||:|||| ||||| |:|||||@LB@ AGAGUAGAAGU UUCAU CUUACCG@LB@3'-AUU...GUGAA UA AAGCA...CAA-5'@LB@ | |@LB@ 109 83@LB@@LB@@LB@Energy -10.90 kcal/mol@LB@Hybridization Energy -24.2284 kcal/mol@LB@Unfolding Energy - mRNA 7.40636 kcal/mol@LB@Unfolding Energy - sRNA 9.09036 kcal/mol@LB@@LB@Position - mRNA 70 -- 106@LB@Position - sRNA 84 -- 108@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 84 -- 90@LB@ |
| 132 |
0.006133 |
0.1741 |
bsu24800 |
yqgW |
-15.55 |
0.025469 |
1 -- 63 |
31 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
______________1_________________________________________________________________64\n______________|_________________________________________________________________|\n___________5'-__G____C_____________CCUACU____U____C________ACUA____CA___C_______UUUAU...CCG-3'\n______________GC_UUCG_UUG_AAUC__GGC______GGCA_UCGU_UCCAUUUC____UUAU__CCU_UUUCUUU\n______________||_||||_|||_||||__|||______::||_||||_:||||:||____||||__||:_|||||||\n______________CG_AAGC_AAC_UUAG__CCG______UUGU_AGCA_GGGUAGAG____AAUA__GGG_AAAGAAA\n3'-AUU...CUUAC___________G____AA___U_________U____U________________AG___U_______CUUUA...CAA-5'\n_____________|__________________________________________________________________|\n_____________86_________________________________________________________________30\n |
36 -- 42 |
48 -- 54 |
-38.6272 kcal/mol |
10.4973 kcal/mol |
14.833 kcal/mol |
1 64@LB@ | |@LB@ 5'- G C CCUACU U C ACUA CA C UUUAU...CCG-3'@LB@ GC UUCG UUG AAUC GGC GGCA UCGU UCCAUUUC UUAU CCU UUUCUUU@LB@ || |||| ||| |||| ||| ::|| |||| :||||:|| |||| ||: |||||||@LB@ CG AAGC AAC UUAG CCG UUGU AGCA GGGUAGAG AAUA GGG AAAGAAA@LB@3'-AUU...CUUAC G AA U U U AG U CUUUA...CAA-5'@LB@ | |@LB@ 86 30@LB@@LB@@LB@Energy -15.55 kcal/mol@LB@Hybridization Energy -38.6272 kcal/mol@LB@Unfolding Energy - mRNA 10.4973 kcal/mol@LB@Unfolding Energy - sRNA 14.833 kcal/mol@LB@@LB@Position - mRNA 1 -- 63@LB@Position - sRNA 31 -- 85@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 133 |
0.006226 |
0.1741 |
bsu14430 |
ykpA |
-10.78 |
0.228396 |
48 -- 105 |
75 -- 129 |
ABC transporter ATP-binding protein |
|
_____________47____________106\n_____________|_____________|\n5'-GUA...AAGUU_____________AAUAU...UUG-3'\n______________AUUUGAAGAUGUU\n______________|||||||:|:|:|\n______________UAAACUUUUGCGA\n3'-AUU...AACUU_____________AUUAA...CAA-5'\n_____________|_____________|\n_____________130___________74\n |
254 -- 260 |
15 -- 21 |
-11.9489 kcal/mol |
0.844332 kcal/mol |
3.96844 kcal/mol |
47 106@LB@ | |@LB@5'-GUA...AAGUU AAUAU...UUG-3'@LB@ AUUUGAAGAUGUU@LB@ |||||||:|:|:|@LB@ UAAACUUUUGCGA@LB@3'-AUU...AACUU AUUAA...CAA-5'@LB@ | |@LB@ 130 74@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -11.9489 kcal/mol@LB@Unfolding Energy - mRNA 0.844332 kcal/mol@LB@Unfolding Energy - sRNA 3.96844 kcal/mol@LB@@LB@Position - mRNA 48 -- 105@LB@Position - sRNA 75 -- 129@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 134 |
0.00626 |
0.1741 |
bsu03360 |
yciC |
-8.59 |
0.518951 |
99 -- 108 |
130 -- 139 |
metal chaperone YciC |
|
_____________98_______109\n_____________|________|\n5'-AGG...GGGCG________AAAUG...UAG-3'\n______________GCAGCGAG\n______________|||||:||\n______________CGUCGUUC\n3'-AUU...ACAGG________AAGGU...CAA-5'\n_____________|________|\n_____________140______129\n |
102 -- 108 |
130 -- 136 |
-12.856 kcal/mol |
2.76877 kcal/mol |
2.9981 kcal/mol |
98 109@LB@ | |@LB@5'-AGG...GGGCG AAAUG...UAG-3'@LB@ GCAGCGAG@LB@ |||||:||@LB@ CGUCGUUC@LB@3'-AUU...ACAGG AAGGU...CAA-5'@LB@ | |@LB@ 140 129@LB@@LB@@LB@Energy -8.59 kcal/mol@LB@Hybridization Energy -12.856 kcal/mol@LB@Unfolding Energy - mRNA 2.76877 kcal/mol@LB@Unfolding Energy - sRNA 2.9981 kcal/mol@LB@@LB@Position - mRNA 99 -- 108@LB@Position - sRNA 130 -- 139@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 130 -- 136@LB@ |
