| 37 |
0.00122 |
0.1206 |
bsu31150 |
yubB |
-14.80 |
0.036692 |
271 -- 292 |
145 -- 165 |
undecaprenyl-diphosphatase |
|
_____________270____________________293\n_____________|______________________|\n5'-UAU...GCCGG____U____________A____CAUAGUAG-3'\n______________UUUC_UCGACAGGACAU_UGAU\n______________:|||_||:|||||||||_|||:\n______________GAAG_AGUUGUCCUGUA_ACUG\n3'-AUU...AAUGA____U_________________UAACA...CAA-5'\n_____________|______________________|\n_____________166____________________144\n |
281 -- 287 |
149 -- 155 |
-24.6442 kcal/mol |
4.4819 kcal/mol |
7.11846 kcal/mol |
270 293@LB@ | |@LB@5'-UAU...GCCGG U A CAUAGUAG-3'@LB@ UUUC UCGACAGGACAU UGAU@LB@ :||| ||:||||||||| |||:@LB@ GAAG AGUUGUCCUGUA ACUG@LB@3'-AUU...AAUGA U UAACA...CAA-5'@LB@ | |@LB@ 166 144@LB@@LB@@LB@Energy -14.80 kcal/mol@LB@Hybridization Energy -24.6442 kcal/mol@LB@Unfolding Energy - mRNA 4.4819 kcal/mol@LB@Unfolding Energy - sRNA 7.11846 kcal/mol@LB@@LB@Position - mRNA 271 -- 292@LB@Position - sRNA 145 -- 165@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 149 -- 155@LB@ |
| 38 |
0.001252 |
0.1206 |
bsu19610 |
yodI |
-17.13 |
0.011794 |
8 -- 27 |
49 -- 68 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________7____________________28\n_____________|____________________|\n____5'-AUUUAUC___________AU_______AUUAC...UAA-3'\n______________GCAAACGAUUG__CCCAUCU\n______________||||||:||:|__|||||||\n______________CGUUUGUUAGC__GGGUAGA\n3'-AUU...AGAAC___________AU_______GAAUA...CAA-5'\n_____________|____________________|\n_____________69___________________48\n |
21 -- 27 |
49 -- 55 |
-26.3851 kcal/mol |
1.36402 kcal/mol |
8.38306 kcal/mol |
7 28@LB@ | |@LB@ 5'-AUUUAUC AU AUUAC...UAA-3'@LB@ GCAAACGAUUG CCCAUCU@LB@ ||||||:||:| |||||||@LB@ CGUUUGUUAGC GGGUAGA@LB@3'-AUU...AGAAC AU GAAUA...CAA-5'@LB@ | |@LB@ 69 48@LB@@LB@@LB@Energy -17.13 kcal/mol@LB@Hybridization Energy -26.3851 kcal/mol@LB@Unfolding Energy - mRNA 1.36402 kcal/mol@LB@Unfolding Energy - sRNA 8.38306 kcal/mol@LB@@LB@Position - mRNA 8 -- 27@LB@Position - sRNA 49 -- 68@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 39 |
0.001272 |
0.1206 |
bsu38090 |
vpr |
-13.65 |
0.063330 |
246 -- 286 |
133 -- 174 |
minor extracellular protease vpr |
|
_____________245__________________________________________287\n_____________|____________________________________________|\n5'-AUU...CUUAU_______G_______C__________ACG______CAG______UCCGG...CAA-3'\n______________UUUUUGC___UUAUC_ACAGG_CAUU___GGCGUU____GCAGC\n______________|||||:|___|:|||_|||||_||||___|:|:||____|||||\n______________AAAAAUG___AGUAG_UGUCC_GUAA___CUGUAA____CGUCG\n3'-AUU...CGAAA_______AGA_____U_____U_____________CAGG_____UUCAA...CAA-5'\n_____________|____________________________________________|\n_____________175__________________________________________132\n |
247 -- 253 |
167 -- 173 |
-30.59 kcal/mol |
9.01512 kcal/mol |
10.7895 kcal/mol |
245 287@LB@ | |@LB@5'-AUU...CUUAU G C ACG CAG UCCGG...CAA-3'@LB@ UUUUUGC UUAUC ACAGG CAUU GGCGUU GCAGC@LB@ |||||:| |:||| ||||| |||| |:|:|| |||||@LB@ AAAAAUG AGUAG UGUCC GUAA CUGUAA CGUCG@LB@3'-AUU...CGAAA AGA U U CAGG UUCAA...CAA-5'@LB@ | |@LB@ 175 132@LB@@LB@@LB@Energy -13.65 kcal/mol@LB@Hybridization Energy -30.59 kcal/mol@LB@Unfolding Energy - mRNA 9.01512 kcal/mol@LB@Unfolding Energy - sRNA 10.7895 kcal/mol@LB@@LB@Position - mRNA 246 -- 286@LB@Position - sRNA 133 -- 174@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 167 -- 173@LB@ |
| 40 |
0.001293 |
0.1206 |
bsu13810 |
ykvS |
-11.02 |
0.206841 |
41 -- 57 |
31 -- 45 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________40________________58\n_____________|_________________|\n5'-UGU...CAAAA_______CCU_______CUAUU...CGA-3'\n______________AUUCCCC___UUUCUUU\n______________|||||||___|||||||\n______________UAAGGGG___AAAGAAA\n3'-AUU...GAGAA_______U_________CUUUA...CAA-5'\n_____________|_________________|\n_____________46________________30\n |
51 -- 57 |
31 -- 37 |
-15.5048 kcal/mol |
1.25691 kcal/mol |
5.1304 kcal/mol |
40 58@LB@ | |@LB@5'-UGU...CAAAA CCU CUAUU...CGA-3'@LB@ AUUCCCC UUUCUUU@LB@ ||||||| |||||||@LB@ UAAGGGG AAAGAAA@LB@3'-AUU...GAGAA U CUUUA...CAA-5'@LB@ | |@LB@ 46 30@LB@@LB@@LB@Energy -11.02 kcal/mol@LB@Hybridization Energy -15.5048 kcal/mol@LB@Unfolding Energy - mRNA 1.25691 kcal/mol@LB@Unfolding Energy - sRNA 5.1304 kcal/mol@LB@@LB@Position - mRNA 41 -- 57@LB@Position - sRNA 31 -- 45@LB@Position Seed - mRNA 51 -- 57@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 41 |
0.00131 |
0.1206 |
bsu01580 |
ybaR |
-15.69 |
0.023818 |
44 -- 97 |
34 -- 84 |
sulfate transporter YbaR |
|
_____________43________________________________________________________________98\n_____________|_________________________________________________________________|\n5'-CAG...UUAUU____UACU______A__A___CUC____CCU___U_____A_______A_GU_____________UCUCC...CGA-3'\n______________AUGA____CUUUGU_UG_CAA___CUUG___CAA_ACAAU__UACUCA_C_____UUCCCCGUUU\n______________||||____|||:||_||_|||___||||___|||_|||||__|||:||_|_____||||||:|||\n______________UACU____GAAGCA_AC_GUU___GAAC___GUU_UGUUA__AUGGGU_G_____AAGGGGUAAA\n3'-AUU...UUCAU____UACC_____________A______C___________GC______A_AGAAU__________GAAAC...CAA-5'\n_____________|_________________________________________________________________|\n_____________85________________________________________________________________33\n |
90 -- 96 |
35 -- 41 |
-34.7178 kcal/mol |
7.59764 kcal/mol |
14.4179 kcal/mol |
43 98@LB@ | |@LB@5'-CAG...UUAUU UACU A A CUC CCU U A A GU UCUCC...CGA-3'@LB@ AUGA CUUUGU UG CAA CUUG CAA ACAAU UACUCA C UUCCCCGUUU@LB@ |||| |||:|| || ||| |||| ||| ||||| |||:|| | ||||||:|||@LB@ UACU GAAGCA AC GUU GAAC GUU UGUUA AUGGGU G AAGGGGUAAA@LB@3'-AUU...UUCAU UACC A C GC A AGAAU GAAAC...CAA-5'@LB@ | |@LB@ 85 33@LB@@LB@@LB@Energy -15.69 kcal/mol@LB@Hybridization Energy -34.7178 kcal/mol@LB@Unfolding Energy - mRNA 7.59764 kcal/mol@LB@Unfolding Energy - sRNA 14.4179 kcal/mol@LB@@LB@Position - mRNA 44 -- 97@LB@Position - sRNA 34 -- 84@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 43 |
0.00135 |
0.1206 |
bsu10230 |
yhfH |
-10.18 |
0.291514 |
18 -- 110 |
27 -- 139 |
Evidence 6: Doubtful CDS |
|
_____________17_____________________________________________________________________________________________________________________111\n_____________|______________________________________________________________________________________________________________________|\n5'-CAU...GAAAA____CAU________________C_____A______A________________UCCUC_______________UU____AA_____GU___CC___AAUAUA_________C______AAAGC...AUG-3'\n______________CCGC___AGCA___UCCG_AUAA___UGU_ACUUUC____________UAAUG_____CU________AUCUU__CAAA__AAUUG__CCU__UUC______CCCAUUUUU_UUGAAA\n______________||||___||||___|||:_||||___|||_||||||____________|||||_____||________|||||__||||__|||:|__||:__:||______|||||||:|_||||||\n______________GGCG___UCGU___AGGU_UAUU___ACA_UGAAAG____________AUUAC_____GA________UAGAA__GUUU__UUAGC__GGG__GAG______GGGUAAAGA_AACUUU\n3'-AUU...UAACA___________UCA____A____ACA___G______AGUAGAAGUUUC_____UUACC__AGCAACGU_____CC____G______AU___UA___AAUAAG________________AAACU...CAA-5'\n_____________|______________________________________________________________________________________________________________________|\n_____________140____________________________________________________________________________________________________________________26\n |
96 -- 102 |
34 -- 40 |
-47.8615 kcal/mol |
11.539 kcal/mol |
28.2686 kcal/mol |
17 111@LB@ | |@LB@5'-CAU...GAAAA CAU C A A UCCUC UU AA GU CC AAUAUA C AAAGC...AUG-3'@LB@ CCGC AGCA UCCG AUAA UGU ACUUUC UAAUG CU AUCUU CAAA AAUUG CCU UUC CCCAUUUUU UUGAAA@LB@ |||| |||| |||: |||| ||| |||||| ||||| || ||||| |||| |||:| ||: :|| |||||||:| ||||||@LB@ GGCG UCGU AGGU UAUU ACA UGAAAG AUUAC GA UAGAA GUUU UUAGC GGG GAG GGGUAAAGA AACUUU@LB@3'-AUU...UAACA UCA A ACA G AGUAGAAGUUUC UUACC AGCAACGU CC G AU UA AAUAAG AAACU...CAA-5'@LB@ | |@LB@ 140 26@LB@@LB@@LB@Energy -10.18 kcal/mol@LB@Hybridization Energy -47.8615 kcal/mol@LB@Unfolding Energy - mRNA 11.539 kcal/mol@LB@Unfolding Energy - sRNA 28.2686 kcal/mol@LB@@LB@Position - mRNA 18 -- 110@LB@Position - sRNA 27 -- 139@LB@Position Seed - mRNA 96 -- 102@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 44 |
0.001404 |
0.1206 |
bsu30660 |
ytkA |
-11.27 |
0.185588 |
215 -- 238 |
164 -- 186 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type lp: lipoprotein' |
|
_____________214_________239\n_____________|___________|\n5'-CAU...GCGCU___________GCGUU...UUU-3'\n______________AAACGUUUAAU\n______________|||||:|||||\n______________UUUGCGAAUUA\n3'-AUU...AAACU___________AAGAA...CAA-5'\n_____________|___________|\n_____________187_________163\n |
23 -- 29 |
11 -- 17 |
-8.86173 kcal/mol |
2.03186 kcal/mol |
2.83264 kcal/mol |
214 239@LB@ | |@LB@5'-CAU...GCGCU GCGUU...UUU-3'@LB@ AAACGUUUAAU@LB@ |||||:|||||@LB@ UUUGCGAAUUA@LB@3'-AUU...AAACU AAGAA...CAA-5'@LB@ | |@LB@ 187 163@LB@@LB@@LB@Energy -11.27 kcal/mol@LB@Hybridization Energy -8.86173 kcal/mol@LB@Unfolding Energy - mRNA 2.03186 kcal/mol@LB@Unfolding Energy - sRNA 2.83264 kcal/mol@LB@@LB@Position - mRNA 215 -- 238@LB@Position - sRNA 164 -- 186@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 11 -- 17@LB@ |
| 45 |
0.001428 |
0.1206 |
bsu27290 |
yrzA |
-10.49 |
0.257926 |
1 -- 17 |
111 -- 136 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________0_________________18\n_____________|_________________|\n_______5'-CGGC_________________ACUCC...ACA-3'\n______________CCAUAU____UUGUCAC\n______________||||||____|||||||\n______________GGUAUA____AACAGUG\n3'-AUU...UUCAA______UUAC_______AAAGA...CAA-5'\n_____________|_________________|\n_____________137_______________110\n |
11 -- 17 |
111 -- 117 |
-14.7924 kcal/mol |
0.950105 kcal/mol |
3.76381 kcal/mol |
0 18@LB@ | |@LB@ 5'-CGGC ACUCC...ACA-3'@LB@ CCAUAU UUGUCAC@LB@ |||||| |||||||@LB@ GGUAUA AACAGUG@LB@3'-AUU...UUCAA UUAC AAAGA...CAA-5'@LB@ | |@LB@ 137 110@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -14.7924 kcal/mol@LB@Unfolding Energy - mRNA 0.950105 kcal/mol@LB@Unfolding Energy - sRNA 3.76381 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 111 -- 136@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 46 |
0.001434 |
0.1206 |
bsu07700 |
nagP |
-14.91 |
0.034715 |
11 -- 49 |
31 -- 62 |
PTS system-N-acetylglucosamine-specific transporter subunit EIICB |
|
_____________10______________________________________50\n_____________|_______________________________________|\n5'-UCU...UUCUU______UUGUUACAA______G_C______GC_______UUAAA...UAU-3'\n______________GAUCGU_________UCAUCU_U_AUUCCC__UUUCUUU\n______________:|||||_________:|||||_|_||||||__|||||||\n______________UUAGCA_________GGUAGA_A_UAAGGG__AAAGAAA\n3'-AUU...GUUUG______UG_____________G_A______GU_______CUUUA...CAA-5'\n_____________|_______________________________________|\n_____________63______________________________________30\n |
43 -- 49 |
31 -- 37 |
-26.4462 kcal/mol |
2.72756 kcal/mol |
10.7322 kcal/mol |
10 50@LB@ | |@LB@5'-UCU...UUCUU UUGUUACAA G C GC UUAAA...UAU-3'@LB@ GAUCGU UCAUCU U AUUCCC UUUCUUU@LB@ :||||| :||||| | |||||| |||||||@LB@ UUAGCA GGUAGA A UAAGGG AAAGAAA@LB@3'-AUU...GUUUG UG G A GU CUUUA...CAA-5'@LB@ | |@LB@ 63 30@LB@@LB@@LB@Energy -14.91 kcal/mol@LB@Hybridization Energy -26.4462 kcal/mol@LB@Unfolding Energy - mRNA 2.72756 kcal/mol@LB@Unfolding Energy - sRNA 10.7322 kcal/mol@LB@@LB@Position - mRNA 11 -- 49@LB@Position - sRNA 31 -- 62@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 49 |
0.001587 |
0.1212 |
bsu40240 |
yycS |
-16.07 |
0.019817 |
5 -- 79 |
35 -- 113 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type h: extrachromosomal origin' |
|
_____________4_________________________________________________________________________________80\n_____________|_________________________________________________________________________________|\n_______5'-AAUA_____G______G______UCCUCC____U_______C__U_____________A___U___U____AA___AA_______AUUCA...GCA-3'\n______________CGCUU_UUUCAU_UUCAAA______GAUG_UUUC_UU_CA_UUUU_____ACAA_UGU_CUC_UCUC__UAU__CCUAUUU\n______________|:|||_|:||||_||||||______:|||_:|||_||_||_|:||_____||||_:||_|:|_||||__|||__||:||||\n______________GUGAA_AGAGUA_AAGUUU______UUAC_GAAG_AA_GU_AGAA_____UGUU_GCA_GGG_AGAG__AUA__GGGUAAA\n3'-AUU...CAACA____________G______CAUUAC____C____C__C__U____CCGUU____A___U___U____A____AG_______GAAAC...CAA-5'\n_____________|_________________________________________________________________________________|\n_____________114_______________________________________________________________________________34\n |
73 -- 79 |
35 -- 41 |
-40.7208 kcal/mol |
6.49365 kcal/mol |
21.9183 kcal/mol |
4 80@LB@ | |@LB@ 5'-AAUA G G UCCUCC U C U A U U AA AA AUUCA...GCA-3'@LB@ CGCUU UUUCAU UUCAAA GAUG UUUC UU CA UUUU ACAA UGU CUC UCUC UAU CCUAUUU@LB@ |:||| |:|||| |||||| :||| :||| || || |:|| |||| :|| |:| |||| ||| ||:||||@LB@ GUGAA AGAGUA AAGUUU UUAC GAAG AA GU AGAA UGUU GCA GGG AGAG AUA GGGUAAA@LB@3'-AUU...CAACA G CAUUAC C C C U CCGUU A U U A AG GAAAC...CAA-5'@LB@ | |@LB@ 114 34@LB@@LB@@LB@Energy -16.07 kcal/mol@LB@Hybridization Energy -40.7208 kcal/mol@LB@Unfolding Energy - mRNA 6.49365 kcal/mol@LB@Unfolding Energy - sRNA 21.9183 kcal/mol@LB@@LB@Position - mRNA 5 -- 79@LB@Position - sRNA 35 -- 113@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 35 -- 41@LB@ |
