| 135 |
0.00631 |
0.1741 |
bsu31510 |
yufK |
-14.38 |
0.044651 |
233 -- 280 |
42 -- 85 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________232________________________________________________281\n_____________|__________________________________________________|\n5'-UGC...UUUCC___GA_________CU___UUUU___U____CCUU_________AAC___ACUUU...UGC-3'\n______________GCU___UUGCGAUC___GC____UAG_UCGU____AUCCAUCUC___UUC\n______________|||___||||:|||___||____:|:_||||____|:|||||||___|||\n______________CGA___AACGUUAG___CG____GUU_AGCA____UGGGUAGAG___AAG\n3'-AUU...CUUAC___AGC________AAC__UUU______________________AAU___GGGUA...CAA-5'\n_____________|__________________________________________________|\n_____________86_________________________________________________41\n |
268 -- 274 |
48 -- 54 |
-29.5205 kcal/mol |
6.02241 kcal/mol |
9.1918 kcal/mol |
232 281@LB@ | |@LB@5'-UGC...UUUCC GA CU UUUU U CCUU AAC ACUUU...UGC-3'@LB@ GCU UUGCGAUC GC UAG UCGU AUCCAUCUC UUC@LB@ ||| ||||:||| || :|: |||| |:||||||| |||@LB@ CGA AACGUUAG CG GUU AGCA UGGGUAGAG AAG@LB@3'-AUU...CUUAC AGC AAC UUU AAU GGGUA...CAA-5'@LB@ | |@LB@ 86 41@LB@@LB@@LB@Energy -14.38 kcal/mol@LB@Hybridization Energy -29.5205 kcal/mol@LB@Unfolding Energy - mRNA 6.02241 kcal/mol@LB@Unfolding Energy - sRNA 9.1918 kcal/mol@LB@@LB@Position - mRNA 233 -- 280@LB@Position - sRNA 42 -- 85@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 136 |
0.006329 |
0.1741 |
bsu10210 |
yhfF |
-9.29 |
0.409483 |
117 -- 143 |
37 -- 59 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________116______________144\n_____________|________________|\n5'-AUA...GACGC_______GCA______CGCAC...CCA-3'\n______________UUCCCAU___UUUGAA\n______________|:|||||___||||||\n______________AGGGGUA___AAACUU\n3'-AUU...GAAUA_______AAG______UAAAC...CAA-5'\n_____________|________________|\n_____________60_______________36\n |
101 -- 107 |
37 -- 43 |
-14.7282 kcal/mol |
3.20386 kcal/mol |
5.02573 kcal/mol |
116 144@LB@ | |@LB@5'-AUA...GACGC GCA CGCAC...CCA-3'@LB@ UUCCCAU UUUGAA@LB@ |:||||| ||||||@LB@ AGGGGUA AAACUU@LB@3'-AUU...GAAUA AAG UAAAC...CAA-5'@LB@ | |@LB@ 60 36@LB@@LB@@LB@Energy -9.29 kcal/mol@LB@Hybridization Energy -14.7282 kcal/mol@LB@Unfolding Energy - mRNA 3.20386 kcal/mol@LB@Unfolding Energy - sRNA 5.02573 kcal/mol@LB@@LB@Position - mRNA 117 -- 143@LB@Position - sRNA 37 -- 59@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 137 |
0.006339 |
0.1741 |
bsu39320 |
yxiB |
-11.20 |
0.191268 |
188 -- 222 |
111 -- 147 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________187_________________________223\n_____________|___________________________|\n5'-UAA...GUGUU______UGCAUA___A___________AUUCG...AAA-3'\n______________GCGGCA______GUG_AAUGUUGUUAC\n______________||:|||______:|:_||||||||:||\n______________CGUCGU______UAU_UUACAACAGUG\n3'-AUU...ACAGG______UCAAGG___A___________AAAGA...CAA-5'\n_____________|___________________________|\n_____________148_________________________110\n |
216 -- 222 |
111 -- 117 |
-23.3412 kcal/mol |
7.76229 kcal/mol |
5.28835 kcal/mol |
187 223@LB@ | |@LB@5'-UAA...GUGUU UGCAUA A AUUCG...AAA-3'@LB@ GCGGCA GUG AAUGUUGUUAC@LB@ ||:||| :|: ||||||||:||@LB@ CGUCGU UAU UUACAACAGUG@LB@3'-AUU...ACAGG UCAAGG A AAAGA...CAA-5'@LB@ | |@LB@ 148 110@LB@@LB@@LB@Energy -11.20 kcal/mol@LB@Hybridization Energy -23.3412 kcal/mol@LB@Unfolding Energy - mRNA 7.76229 kcal/mol@LB@Unfolding Energy - sRNA 5.28835 kcal/mol@LB@@LB@Position - mRNA 188 -- 222@LB@Position - sRNA 111 -- 147@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 138 |
0.00639 |
0.1741 |
bsu24900 |
rpmG |
-18.16 |
0.007101 |
92 -- 175 |
92 -- 166 |
50S ribosomal protein L33 |
|
_____________91_________________________________________________________176\n_____________|__________________________________________________________|\n5'-CGC...GCGUA______AUAAU______AUU______AAAACAG________UUCCG____U_______GUAUU...AAA-3'\n______________UCUUCA_____UCAGCA___CAUUGA_______CC_UAGCA_____UCCA_UAUAGUG\n______________||||||_____|||:||___||||||_______||_:||||_____||||_||||:||\n______________AGAAGU_____AGUUGU___GUAACU_______GG_GUCGU_____AGGU_AUAUUAC\n3'-AUU...AAAUG_________________CCU______GUAACA___C_____UCA______________AACAG...CAA-5'\n_____________|__________________________________________________________|\n_____________167________________________________________________________91\n |
142 -- 148 |
118 -- 124 |
-35.8515 kcal/mol |
8.17901 kcal/mol |
10.6225 kcal/mol |
91 176@LB@ | |@LB@5'-CGC...GCGUA AUAAU AUU AAAACAG UUCCG U GUAUU...AAA-3'@LB@ UCUUCA UCAGCA CAUUGA CC UAGCA UCCA UAUAGUG@LB@ |||||| |||:|| |||||| || :|||| |||| ||||:||@LB@ AGAAGU AGUUGU GUAACU GG GUCGU AGGU AUAUUAC@LB@3'-AUU...AAAUG CCU GUAACA C UCA AACAG...CAA-5'@LB@ | |@LB@ 167 91@LB@@LB@@LB@Energy -18.16 kcal/mol@LB@Hybridization Energy -35.8515 kcal/mol@LB@Unfolding Energy - mRNA 8.17901 kcal/mol@LB@Unfolding Energy - sRNA 10.6225 kcal/mol@LB@@LB@Position - mRNA 92 -- 175@LB@Position - sRNA 92 -- 166@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 139 |
0.006395 |
0.1741 |
bsu30170 |
ytcP |
-13.74 |
0.060669 |
6 -- 28 |
1 -- 23 |
ABC transporter permease |
|
_____________5_______________________29\n_____________|_______________________|\n______5'-UCUAA__UU_____CUU___________AUCGG...UUG-3'\n______________GA__AUGCU___UUUCUUUUGUU\n______________||__|:|||___|||||||||||\n______________CU__UGCGA___AAAGAAAACAA\n3'-AUU...UUAAA__UU_____AUU___________-5'\n_____________|______________________|\n_____________24_____________________1\n |
22 -- 28 |
1 -- 7 |
-18.7227 kcal/mol |
2.34195 kcal/mol |
4.12892 kcal/mol |
5 29@LB@ | |@LB@ 5'-UCUAA UU CUU AUCGG...UUG-3'@LB@ GA AUGCU UUUCUUUUGUU@LB@ || |:||| |||||||||||@LB@ CU UGCGA AAAGAAAACAA@LB@3'-AUU...UUAAA UU AUU -5'@LB@ | |@LB@ 24 1@LB@@LB@@LB@Energy -13.74 kcal/mol@LB@Hybridization Energy -18.7227 kcal/mol@LB@Unfolding Energy - mRNA 2.34195 kcal/mol@LB@Unfolding Energy - sRNA 4.12892 kcal/mol@LB@@LB@Position - mRNA 6 -- 28@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 1 -- 7@LB@ |
| 140 |
0.006451 |
0.1741 |
bsu29090 |
polA |
-11.51 |
0.167440 |
58 -- 84 |
30 -- 56 |
DNA polymerase I |
|
_____________57________________85\n_____________|_________________|\n5'-AUG...CGGGA________G________GUCCU...AUA-3'\n______________ACCUAUUU__UGUUCCC\n______________|||:||:|__|:|||||\n______________UGGGUAGA__AUAAGGG\n3'-AUU...UAGCA________GA_______GUAAA...CAA-5'\n_____________|_________________|\n_____________57________________29\n |
67 -- 73 |
40 -- 46 |
-17.5976 kcal/mol |
2.41953 kcal/mol |
7.22212 kcal/mol |
57 85@LB@ | |@LB@5'-AUG...CGGGA G GUCCU...AUA-3'@LB@ ACCUAUUU UGUUCCC@LB@ |||:||:| |:|||||@LB@ UGGGUAGA AUAAGGG@LB@3'-AUU...UAGCA GA GUAAA...CAA-5'@LB@ | |@LB@ 57 29@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -17.5976 kcal/mol@LB@Unfolding Energy - mRNA 2.41953 kcal/mol@LB@Unfolding Energy - sRNA 7.22212 kcal/mol@LB@@LB@Position - mRNA 58 -- 84@LB@Position - sRNA 30 -- 56@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 142 |
0.006636 |
0.1741 |
bsu22510 |
ypjC |
-12.74 |
0.096342 |
122 -- 155 |
156 -- 190 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________121_____________________________________________________________________________156\n_____________|_______________________________________________________________________________|\n5'-UAA...UCAUC_____A___GUUAGCUAAUU___AU___U______AA_____A_____C_____________C____UU__________CACUA...UGG-3'\n______________CUCUU_CAU___________CAG__ACG_UUGACA__UGUUU__AGCA_GUU________UG_UGUU__CUUUUUCAUC\n______________|||||_|||___________|||__||:_||||||__|||::__||||_|||________||_|||:__||||:|||||\n______________GAGAA_GUA___________GUC__UGU_AACUGU__ACAGG__UCGU_CAA________AC_ACAG__GAAAGAGUAG\n3'-AUU...AAAAU_________GUU___________C___________A______CG____U___GGUAUAUU__A____U___________AAGUU...CAA-5'\n_____________|_______________________________________________________________________________|\n_____________191_____________________________________________________________________________155\n |
146 -- 152 |
102 -- 108 |
-35.2093 kcal/mol |
10.3676 kcal/mol |
14.8184 kcal/mol |
121 156@LB@ | |@LB@5'-UAA...UCAUC A GUUAGCUAAUU AU U AA A C C UU CACUA...UGG-3'@LB@ CUCUU CAU CAG ACG UUGACA UGUUU AGCA GUU UG UGUU CUUUUUCAUC@LB@ ||||| ||| ||| ||: |||||| |||:: |||| ||| || |||: ||||:|||||@LB@ GAGAA GUA GUC UGU AACUGU ACAGG UCGU CAA AC ACAG GAAAGAGUAG@LB@3'-AUU...AAAAU GUU C A CG U GGUAUAUU A U AAGUU...CAA-5'@LB@ | |@LB@ 191 155@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -35.2093 kcal/mol@LB@Unfolding Energy - mRNA 10.3676 kcal/mol@LB@Unfolding Energy - sRNA 14.8184 kcal/mol@LB@@LB@Position - mRNA 122 -- 155@LB@Position - sRNA 156 -- 190@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 143 |
0.006663 |
0.1741 |
bsu05790 |
ydhK |
-18.88 |
0.004963 |
179 -- 222 |
125 -- 163 |
Evidence 5: No homology to any previously reported sequences |
|
_____________178__________________________________________223\n_____________|____________________________________________|\n5'-GUG...UAGUA_______G_______GUUUU____________G__AA___UA__GGAAA...UUU-3'\n______________UUAUCGA_AGGGCAU_____CAUUGUCUGCGG_CA__AGU__CA\n______________|:|||:|_|||:|||_____|||||||:||:|_||__|||__||\n______________AGUAGUU_UCCUGUA_____GUAACAGGCGUC_GU__UCA__GU\n3'-AUU...UGAGA_______G_______ACU______________________AG__AUAUU...CAA-5'\n_____________|____________________________________________|\n_____________164__________________________________________124\n |
204 -- 210 |
134 -- 140 |
-40.9125 kcal/mol |
11.6904 kcal/mol |
10.3584 kcal/mol |
178 223@LB@ | |@LB@5'-GUG...UAGUA G GUUUU G AA UA GGAAA...UUU-3'@LB@ UUAUCGA AGGGCAU CAUUGUCUGCGG CA AGU CA@LB@ |:|||:| |||:||| |||||||:||:| || ||| ||@LB@ AGUAGUU UCCUGUA GUAACAGGCGUC GU UCA GU@LB@3'-AUU...UGAGA G ACU AG AUAUU...CAA-5'@LB@ | |@LB@ 164 124@LB@@LB@@LB@Energy -18.88 kcal/mol@LB@Hybridization Energy -40.9125 kcal/mol@LB@Unfolding Energy - mRNA 11.6904 kcal/mol@LB@Unfolding Energy - sRNA 10.3584 kcal/mol@LB@@LB@Position - mRNA 179 -- 222@LB@Position - sRNA 125 -- 163@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 134 -- 140@LB@ |
| 144 |
0.006712 |
0.1741 |
bsu25040 |
yqgB |
-19.28 |
0.004052 |
60 -- 160 |
55 -- 168 |
Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 15294815; Product type ph: phenotype |
|
_____________59_____________________________________________________161\n_____________|______________________________________________________|\n5'-UAA...AAAAC__C_______AACA__CAAU______A_______AG___CCAUCC_________ACUCU...UUU-3'\n______________CG_UGCAAUC____GC____ACAA_C_UAUCCAU__CUU______UCCCAUUUU\n______________||_|||||||____||____||||_|_||:||||__|||______:|||||||:\n______________GC_ACGUUAG____CG____UGUU_G_AUGGGUA__GAA______GGGGUAAAG\n3'-AUU...CCGAA__A_______AAC___UU______A_C_______GA___UAA____________AAACU...CAA-5'\n_____________|______________________________________________________|\n_____________169____________________________________________________54\n |
105 -- 111 |
34 -- 40 |
-33.5165 kcal/mol |
5.00067 kcal/mol |
13.93 kcal/mol |
59 161@LB@ | |@LB@5'-UAA...AAAAC C AACA CAAU A AG CCAUCC ACUCU...UUU-3'@LB@ CG UGCAAUC GC ACAA C UAUCCAU CUU UCCCAUUUU@LB@ || ||||||| || |||| | ||:|||| ||| :|||||||:@LB@ GC ACGUUAG CG UGUU G AUGGGUA GAA GGGGUAAAG@LB@3'-AUU...CCGAA A AAC UU A C GA UAA AAACU...CAA-5'@LB@ | |@LB@ 169 54@LB@@LB@@LB@Energy -19.28 kcal/mol@LB@Hybridization Energy -33.5165 kcal/mol@LB@Unfolding Energy - mRNA 5.00067 kcal/mol@LB@Unfolding Energy - sRNA 13.93 kcal/mol@LB@@LB@Position - mRNA 60 -- 160@LB@Position - sRNA 55 -- 168@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 146 |
0.00677 |
0.1741 |
bsu29690 |
acuA |
-9.85 |
0.331997 |
126 -- 137 |
14 -- 25 |
acetoin utilization protein AcuA |
|
_____________125____________138\n_____________|______________|\n5'-UAU...AUUCA______________UUUUA...AUG-3'\n______________UAUUCUUC_UCAAU\n______________||:|||||_||||:\n______________AUGAGAAG_AGUUG\n3'-AUU...AAAAA________U_____UCCUG...CAA-5'\n_____________|______________|\n_____________26_____________13\n |
95 -- 101 |
162 -- 168 |
-12.3213 kcal/mol |
1.49895 kcal/mol |
1.40683 kcal/mol |
125 138@LB@ | |@LB@5'-UAU...AUUCA UUUUA...AUG-3'@LB@ UAUUCUUC UCAAU@LB@ ||:||||| ||||:@LB@ AUGAGAAG AGUUG@LB@3'-AUU...AAAAA U UCCUG...CAA-5'@LB@ | |@LB@ 26 13@LB@@LB@@LB@Energy -9.85 kcal/mol@LB@Hybridization Energy -12.3213 kcal/mol@LB@Unfolding Energy - mRNA 1.49895 kcal/mol@LB@Unfolding Energy - sRNA 1.40683 kcal/mol@LB@@LB@Position - mRNA 126 -- 137@LB@Position - sRNA 14 -- 25@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 162 -- 168@LB@ |
