| 74 |
0.002384 |
0.1242 |
bsu09450 |
yhdF |
-14.81 |
0.036487 |
208 -- 244 |
132 -- 159 |
oxidoreductase |
|
_____________207____________________________________245\n_____________|______________________________________|\n5'-UCC...GAACC__________GACAAACAGAAG____AGAA________UCAGG...AGG-3'\n______________CAAU_GGACA____________GACA____CCGCAGCA\n______________|||:_|||||____________||||____||||||||\n______________GUUG_CCUGU____________CUGU____GGCGUCGU\n3'-AUU...AAGUA____U_____AA______________AACA________UCAAG...CAA-5'\n_____________|______________________________________|\n_____________160____________________________________131\n |
238 -- 244 |
132 -- 138 |
-26.7883 kcal/mol |
3.14586 kcal/mol |
9.22644 kcal/mol |
207 245@LB@ | |@LB@5'-UCC...GAACC GACAAACAGAAG AGAA UCAGG...AGG-3'@LB@ CAAU GGACA GACA CCGCAGCA@LB@ |||: ||||| |||| ||||||||@LB@ GUUG CCUGU CUGU GGCGUCGU@LB@3'-AUU...AAGUA U AA AACA UCAAG...CAA-5'@LB@ | |@LB@ 160 131@LB@@LB@@LB@Energy -14.81 kcal/mol@LB@Hybridization Energy -26.7883 kcal/mol@LB@Unfolding Energy - mRNA 3.14586 kcal/mol@LB@Unfolding Energy - sRNA 9.22644 kcal/mol@LB@@LB@Position - mRNA 208 -- 244@LB@Position - sRNA 132 -- 159@LB@Position Seed - mRNA 238 -- 244@LB@Position Seed - sRNA 132 -- 138@LB@ |
| 152 |
0.006831 |
0.1741 |
bsu09480 |
yhdI |
-9.89 |
0.327778 |
76 -- 101 |
102 -- 130 |
GntR family transcriptional regulator |
bsu05240 |
_____________75__________________________102\n_____________|___________________________|\n5'-UCU...UAAUU___________A________C______AUCCC...CAA-3'\n______________GUUUCAUAUGG_____UCAU_UUCUCA\n______________|||:|||||::_____|||:_||||||\n______________CAAGGUAUAUU_____AGUG_AAGAGU\n3'-AUU...UCGUU___________ACAAC____A______AGAAG...CAA-5'\n_____________|___________________________|\n_____________131_________________________101\n |
79 -- 85 |
121 -- 127 |
-18.1477 kcal/mol |
2.87073 kcal/mol |
6.25825 kcal/mol |
75 102@LB@ | |@LB@5'-UCU...UAAUU A C AUCCC...CAA-3'@LB@ GUUUCAUAUGG UCAU UUCUCA@LB@ |||:|||||:: |||: ||||||@LB@ CAAGGUAUAUU AGUG AAGAGU@LB@3'-AUU...UCGUU ACAAC A AGAAG...CAA-5'@LB@ | |@LB@ 131 101@LB@@LB@@LB@Energy -9.89 kcal/mol@LB@Hybridization Energy -18.1477 kcal/mol@LB@Unfolding Energy - mRNA 2.87073 kcal/mol@LB@Unfolding Energy - sRNA 6.25825 kcal/mol@LB@@LB@Position - mRNA 76 -- 101@LB@Position - sRNA 102 -- 130@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 103 |
0.004238 |
0.1594 |
bsu09690 |
nhaX |
-18.33 |
0.006526 |
38 -- 80 |
34 -- 77 |
stress response protein NhaX |
|
_____________37________________________________________________81\n_____________|_________________________________________________|\n5'-GGU...AACAC____AUUC_______U____________________CCA____G_____AUCUU...CCG-3'\n______________GCAA____CUUGGCA_AGCAA____ACCC_UC_CUU___UUCU_AUUUC\n______________||||____|||||||_|:|||____||||_||_|||___|:|:_|||||\n______________CGUU____GAACCGU_UUGUU____UGGG_AG_GAA___AGGG_UAAAG\n3'-AUU...AGCAA____A________________AGCA____U__A___UA_____G_____AAACU...CAA-5'\n_____________|_________________________________________________|\n_____________78________________________________________________33\n |
46 -- 52 |
66 -- 72 |
-32.0538 kcal/mol |
3.14542 kcal/mol |
13.7939 kcal/mol |
37 81@LB@ | |@LB@5'-GGU...AACAC AUUC U CCA G AUCUU...CCG-3'@LB@ GCAA CUUGGCA AGCAA ACCC UC CUU UUCU AUUUC@LB@ |||| ||||||| |:||| |||| || ||| |:|: |||||@LB@ CGUU GAACCGU UUGUU UGGG AG GAA AGGG UAAAG@LB@3'-AUU...AGCAA A AGCA U A UA G AAACU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -18.33 kcal/mol@LB@Hybridization Energy -32.0538 kcal/mol@LB@Unfolding Energy - mRNA 3.14542 kcal/mol@LB@Unfolding Energy - sRNA 13.7939 kcal/mol@LB@@LB@Position - mRNA 38 -- 80@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 167 |
0.007775 |
0.1751 |
bsu09889 |
yhzD |
-15.75 |
0.023145 |
43 -- 92 |
39 -- 89 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________42____________________________________________________93\n_____________|_____________________________________________________|\n5'-GGA...CUCCA____U_______AC______AAUU_______C___A____C____C_______UGACC...GUG-3'\n______________AAUG_CUUCGUU__GUGAUC____GUAAGCA__UG_AUCU__CUC___UUCCC\n______________||||_|||||||__|::|||____|:||:||__:|_|:|:__|||___|||||\n______________UUAC_GAAGCAA__CGUUAG____CGUUUGU__GC_UGGG__GAG___AAGGG\n3'-AUU...AUUAC____C_______________AAC________UA__A____UA___AAU_____GUAAA...CAA-5'\n_____________|_____________________________________________________|\n_____________90____________________________________________________38\n |
67 -- 73 |
62 -- 68 |
-30.4166 kcal/mol |
5.34264 kcal/mol |
10.926 kcal/mol |
42 93@LB@ | |@LB@5'-GGA...CUCCA U AC AAUU C A C C UGACC...GUG-3'@LB@ AAUG CUUCGUU GUGAUC GUAAGCA UG AUCU CUC UUCCC@LB@ |||| ||||||| |::||| |:||:|| :| |:|: ||| |||||@LB@ UUAC GAAGCAA CGUUAG CGUUUGU GC UGGG GAG AAGGG@LB@3'-AUU...AUUAC C AAC UA A UA AAU GUAAA...CAA-5'@LB@ | |@LB@ 90 38@LB@@LB@@LB@Energy -15.75 kcal/mol@LB@Hybridization Energy -30.4166 kcal/mol@LB@Unfolding Energy - mRNA 5.34264 kcal/mol@LB@Unfolding Energy - sRNA 10.926 kcal/mol@LB@@LB@Position - mRNA 43 -- 92@LB@Position - sRNA 39 -- 89@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 110 |
0.004746 |
0.1671 |
bsu09940 |
yhaL |
-13.90 |
0.056226 |
141 -- 159 |
37 -- 56 |
sporulation protein YhaL |
|
_____________140______________________________________160\n_____________|________________________________________|\n5'-GAA...UUUUU____________GUA___U____U___A_GCA________AGCGC...ACC-3'\n______________UCCGUGGUGGGU___UCU_UGUA_UGU_G___UCAUUUUC\n______________||||::|:::||___||:_|:||_|||_|___|||:||||\n______________AGGCGUCGUUCA___AGG_AUAU_ACA_C___AGUGAAAG\n3'-AUU...GUAAC__________________U____U___A____________AGUAG...CAA-5'\n_____________|________________________________________|\n_____________57_______________________________________36\n |
245 -- 251 |
107 -- 113 |
-27.9954 kcal/mol |
7.44184 kcal/mol |
9.97721 kcal/mol |
140 160@LB@ | |@LB@5'-GAA...UUUUU GUA U U A GCA AGCGC...ACC-3'@LB@ UCCGUGGUGGGU UCU UGUA UGU G UCAUUUUC@LB@ ||||::|:::|| ||: |:|| ||| | |||:||||@LB@ AGGCGUCGUUCA AGG AUAU ACA C AGUGAAAG@LB@3'-AUU...GUAAC U U A AGUAG...CAA-5'@LB@ | |@LB@ 57 36@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -27.9954 kcal/mol@LB@Unfolding Energy - mRNA 7.44184 kcal/mol@LB@Unfolding Energy - sRNA 9.97721 kcal/mol@LB@@LB@Position - mRNA 141 -- 159@LB@Position - sRNA 37 -- 56@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 22 |
0.0005922 |
0.1011 |
bsu09965 |
yhaJ |
-9.39 |
0.394896 |
264 -- 275 |
174 -- 185 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 16845009; Product type pf : factor' |
|
_____________263___________276\n_____________|_____________|\n5'-ACU...AACCC___AU________UUGUU...CCU-3'\n______________CAU__UUCGCAGU\n______________|||__|:|||||:\n______________GUA__AGGCGUCG\n3'-AUU...UAACU___AC________UUCAA...CAA-5'\n_____________|_____________|\n_____________186___________173\n |
68 -- 74 |
133 -- 139 |
-12.4018 kcal/mol |
1.72698 kcal/mol |
5.12932 kcal/mol |
263 276@LB@ | |@LB@5'-ACU...AACCC AU UUGUU...CCU-3'@LB@ CAU UUCGCAGU@LB@ ||| |:|||||:@LB@ GUA AGGCGUCG@LB@3'-AUU...UAACU AC UUCAA...CAA-5'@LB@ | |@LB@ 186 173@LB@@LB@@LB@Energy -9.39 kcal/mol@LB@Hybridization Energy -12.4018 kcal/mol@LB@Unfolding Energy - mRNA 1.72698 kcal/mol@LB@Unfolding Energy - sRNA 5.12932 kcal/mol@LB@@LB@Position - mRNA 264 -- 275@LB@Position - sRNA 174 -- 185@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 133 -- 139@LB@ |
| 53 |
0.001762 |
0.1212 |
bsu10040 |
ecsA |
-7.56 |
0.695140 |
42 -- 51 |
33 -- 42 |
ABC transporter ATP-binding protein |
|
_____________41_________52\n_____________|__________|\n5'-UAU...AAACA__________CAGUU...GCU-3'\n______________CCUCAUUUUU\n______________||:|||||:|\n______________GGGGUAAAGA\n3'-AUU...AAUAA__________AACUU...CAA-5'\n_____________|__________|\n_____________43_________32\n |
45 -- 51 |
33 -- 39 |
-10.6739 kcal/mol |
0.405129 kcal/mol |
4.65815 kcal/mol |
41 52@LB@ | |@LB@5'-UAU...AAACA CAGUU...GCU-3'@LB@ CCUCAUUUUU@LB@ ||:|||||:|@LB@ GGGGUAAAGA@LB@3'-AUU...AAUAA AACUU...CAA-5'@LB@ | |@LB@ 43 32@LB@@LB@@LB@Energy -7.56 kcal/mol@LB@Hybridization Energy -10.6739 kcal/mol@LB@Unfolding Energy - mRNA 0.405129 kcal/mol@LB@Unfolding Energy - sRNA 4.65815 kcal/mol@LB@@LB@Position - mRNA 42 -- 51@LB@Position - sRNA 33 -- 42@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 136 |
0.006329 |
0.1741 |
bsu10210 |
yhfF |
-9.29 |
0.409483 |
117 -- 143 |
37 -- 59 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________116______________144\n_____________|________________|\n5'-AUA...GACGC_______GCA______CGCAC...CCA-3'\n______________UUCCCAU___UUUGAA\n______________|:|||||___||||||\n______________AGGGGUA___AAACUU\n3'-AUU...GAAUA_______AAG______UAAAC...CAA-5'\n_____________|________________|\n_____________60_______________36\n |
101 -- 107 |
37 -- 43 |
-14.7282 kcal/mol |
3.20386 kcal/mol |
5.02573 kcal/mol |
116 144@LB@ | |@LB@5'-AUA...GACGC GCA CGCAC...CCA-3'@LB@ UUCCCAU UUUGAA@LB@ |:||||| ||||||@LB@ AGGGGUA AAACUU@LB@3'-AUU...GAAUA AAG UAAAC...CAA-5'@LB@ | |@LB@ 60 36@LB@@LB@@LB@Energy -9.29 kcal/mol@LB@Hybridization Energy -14.7282 kcal/mol@LB@Unfolding Energy - mRNA 3.20386 kcal/mol@LB@Unfolding Energy - sRNA 5.02573 kcal/mol@LB@@LB@Position - mRNA 117 -- 143@LB@Position - sRNA 37 -- 59@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 43 |
0.00135 |
0.1206 |
bsu10230 |
yhfH |
-10.18 |
0.291514 |
18 -- 110 |
27 -- 139 |
Evidence 6: Doubtful CDS |
|
_____________17_____________________________________________________________________________________________________________________111\n_____________|______________________________________________________________________________________________________________________|\n5'-CAU...GAAAA____CAU________________C_____A______A________________UCCUC_______________UU____AA_____GU___CC___AAUAUA_________C______AAAGC...AUG-3'\n______________CCGC___AGCA___UCCG_AUAA___UGU_ACUUUC____________UAAUG_____CU________AUCUU__CAAA__AAUUG__CCU__UUC______CCCAUUUUU_UUGAAA\n______________||||___||||___|||:_||||___|||_||||||____________|||||_____||________|||||__||||__|||:|__||:__:||______|||||||:|_||||||\n______________GGCG___UCGU___AGGU_UAUU___ACA_UGAAAG____________AUUAC_____GA________UAGAA__GUUU__UUAGC__GGG__GAG______GGGUAAAGA_AACUUU\n3'-AUU...UAACA___________UCA____A____ACA___G______AGUAGAAGUUUC_____UUACC__AGCAACGU_____CC____G______AU___UA___AAUAAG________________AAACU...CAA-5'\n_____________|______________________________________________________________________________________________________________________|\n_____________140____________________________________________________________________________________________________________________26\n |
96 -- 102 |
34 -- 40 |
-47.8615 kcal/mol |
11.539 kcal/mol |
28.2686 kcal/mol |
17 111@LB@ | |@LB@5'-CAU...GAAAA CAU C A A UCCUC UU AA GU CC AAUAUA C AAAGC...AUG-3'@LB@ CCGC AGCA UCCG AUAA UGU ACUUUC UAAUG CU AUCUU CAAA AAUUG CCU UUC CCCAUUUUU UUGAAA@LB@ |||| |||| |||: |||| ||| |||||| ||||| || ||||| |||| |||:| ||: :|| |||||||:| ||||||@LB@ GGCG UCGU AGGU UAUU ACA UGAAAG AUUAC GA UAGAA GUUU UUAGC GGG GAG GGGUAAAGA AACUUU@LB@3'-AUU...UAACA UCA A ACA G AGUAGAAGUUUC UUACC AGCAACGU CC G AU UA AAUAAG AAACU...CAA-5'@LB@ | |@LB@ 140 26@LB@@LB@@LB@Energy -10.18 kcal/mol@LB@Hybridization Energy -47.8615 kcal/mol@LB@Unfolding Energy - mRNA 11.539 kcal/mol@LB@Unfolding Energy - sRNA 28.2686 kcal/mol@LB@@LB@Position - mRNA 18 -- 110@LB@Position - sRNA 27 -- 139@LB@Position Seed - mRNA 96 -- 102@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 31 |
0.0008564 |
0.1059 |
bsu10290 |
yhfN |
-10.46 |
0.260955 |
32 -- 88 |
131 -- 186 |
metalloprotease YhfN |
|
_____________31__________________89\n_____________|___________________|\n5'-AUG...GCAAG__________G________ACUUG...AAG-3'\n______________UGG_AUUG_C_GCAGCAGG\n______________||:_||||_|_||||||:|\n______________ACU_UAAC_G_CGUCGUUC\n3'-AUU...CUGUA___G____A_G________AAGGU...CAA-5'\n_____________|___________________|\n_____________187_________________130\n |
220 -- 226 |
130 -- 136 |
-18.5522 kcal/mol |
5.78917 kcal/mol |
6.22375 kcal/mol |
31 89@LB@ | |@LB@5'-AUG...GCAAG G ACUUG...AAG-3'@LB@ UGG AUUG C GCAGCAGG@LB@ ||: |||| | ||||||:|@LB@ ACU UAAC G CGUCGUUC@LB@3'-AUU...CUGUA G A G AAGGU...CAA-5'@LB@ | |@LB@ 187 130@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -18.5522 kcal/mol@LB@Unfolding Energy - mRNA 5.78917 kcal/mol@LB@Unfolding Energy - sRNA 6.22375 kcal/mol@LB@@LB@Position - mRNA 32 -- 88@LB@Position - sRNA 131 -- 186@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 130 -- 136@LB@ |
