| 192 |
0.00876 |
0.1751 |
bsu33520 |
csoR |
-16.67 |
0.014798 |
32 -- 56 |
39 -- 62 |
copper-sensing transcriptional repressor CsoR |
|
_____________31_________________________57\n_____________|__________________________|\n5'-CGG...ACCUU___________CU______C______UUUAA...GAG-3'\n______________GAUCGUAUCCA__UUUCUU__UCCCC\n______________:||||||:|||__|:||||__|||||\n______________UUAGCAUGGGU__AGAGAA__AGGGG\n3'-AUU...GUUUG___________________UA_____UAAAG...CAA-5'\n_____________|__________________________|\n_____________63_________________________38\n |
33 -- 39 |
55 -- 61 |
-27.3317 kcal/mol |
3.5392 kcal/mol |
9.04037 kcal/mol |
31 57@LB@ | |@LB@5'-CGG...ACCUU CU C UUUAA...GAG-3'@LB@ GAUCGUAUCCA UUUCUU UCCCC@LB@ :||||||:||| |:|||| |||||@LB@ UUAGCAUGGGU AGAGAA AGGGG@LB@3'-AUU...GUUUG UA UAAAG...CAA-5'@LB@ | |@LB@ 63 38@LB@@LB@@LB@Energy -16.67 kcal/mol@LB@Hybridization Energy -27.3317 kcal/mol@LB@Unfolding Energy - mRNA 3.5392 kcal/mol@LB@Unfolding Energy - sRNA 9.04037 kcal/mol@LB@@LB@Position - mRNA 32 -- 56@LB@Position - sRNA 39 -- 62@LB@Position Seed - mRNA 33 -- 39@LB@Position Seed - sRNA 55 -- 61@LB@ |
| 197 |
0.009002 |
0.1751 |
bsu33650 |
yvzC |
-16.51 |
0.015985 |
3 -- 52 |
121 -- 172 |
HTH-type transcriptional regulator YvzC |
|
_____________2__________________________________________________53\n_____________|__________________________________________________|\n____5'-ACUUUCU_______C_____AU__CC__UA_________CAA___U___________UCUCA...UCU-3'\n______________CUCUUCA___GCA__GC___G__AUUGUUCGC___AGC___UUCCAUAUA\n______________|||||||___:||__:|___|__|||||:|||___|||___|||||||||\n______________GAGAAGU___UGU__UG___C__UAACAGGCG___UCG___AAGGUAUAU\n3'-AUU...AAAAU_______AGU___CC__UAA_UG_______________UUC_________UACAA...CAA-5'\n_____________|__________________________________________________|\n_____________173________________________________________________120\n |
46 -- 52 |
121 -- 127 |
-33.6584 kcal/mol |
6.173 kcal/mol |
10.5345 kcal/mol |
2 53@LB@ | |@LB@ 5'-ACUUUCU C AU CC UA CAA U UCUCA...UCU-3'@LB@ CUCUUCA GCA GC G AUUGUUCGC AGC UUCCAUAUA@LB@ ||||||| :|| :| | |||||:||| ||| |||||||||@LB@ GAGAAGU UGU UG C UAACAGGCG UCG AAGGUAUAU@LB@3'-AUU...AAAAU AGU CC UAA UG UUC UACAA...CAA-5'@LB@ | |@LB@ 173 120@LB@@LB@@LB@Energy -16.51 kcal/mol@LB@Hybridization Energy -33.6584 kcal/mol@LB@Unfolding Energy - mRNA 6.173 kcal/mol@LB@Unfolding Energy - sRNA 10.5345 kcal/mol@LB@@LB@Position - mRNA 3 -- 52@LB@Position - sRNA 121 -- 172@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 48 |
0.001494 |
0.1206 |
bsu33660 |
rghRA |
-13.24 |
0.076349 |
28 -- 146 |
39 -- 166 |
HTH-type transcriptional repressor RghR |
bsu33670 |
_____________27_________________________________________________147\n_____________|__________________________________________________|\n5'-UAU...AUUAC_____A____AAC______U___U___U_______A____A_C_______UUUAG...UCU-3'\n______________GGCUU_UGUU____UUUUG_CAA_ACA_UU___CC_GUCU_C_UAUUCCC\n______________|||||_:|||____|:|||_|||_|||_|:___||_:|||_|_|||||||\n______________CCGAA_GCAA____AGAAC_GUU_UGU_AG___GG_UAGA_G_AUAAGGG\n3'-AUU...ACUUA__________CGUU_____C_______U__CAU__G______A_______GUAAA...CAA-5'\n_____________|__________________________________________________|\n_____________167________________________________________________38\n |
139 -- 145 |
40 -- 46 |
-27.8785 kcal/mol |
6.21563 kcal/mol |
10.9223 kcal/mol |
27 147@LB@ | |@LB@5'-UAU...AUUAC A AAC U U U A A C UUUAG...UCU-3'@LB@ GGCUU UGUU UUUUG CAA ACA UU CC GUCU C UAUUCCC@LB@ ||||| :||| |:||| ||| ||| |: || :||| | |||||||@LB@ CCGAA GCAA AGAAC GUU UGU AG GG UAGA G AUAAGGG@LB@3'-AUU...ACUUA CGUU C U CAU G A GUAAA...CAA-5'@LB@ | |@LB@ 167 38@LB@@LB@@LB@Energy -13.24 kcal/mol@LB@Hybridization Energy -27.8785 kcal/mol@LB@Unfolding Energy - mRNA 6.21563 kcal/mol@LB@Unfolding Energy - sRNA 10.9223 kcal/mol@LB@@LB@Position - mRNA 28 -- 146@LB@Position - sRNA 39 -- 166@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 5 |
0.0001198 |
0.08823 |
bsu33680 |
yvaP |
-23.88 |
0.000393 |
26 -- 134 |
34 -- 167 |
HTH-type transcriptional regulator YvaP |
|
_____________25___________________________________________________________________________________________________________________________________________________________135\n_____________|____________________________________________________________________________________________________________________________________________________________|\n5'-UUU...AACAU_______A______________AAA____________AUA________________A_______________AGCAAAAAAAC__________C_____C__UC______C___________CU____A______AUCAAUC______________CAUGC...CGC-3'\n______________CUC_UCA__AGCAGGAUAUUGA_____UCU_CAGCAA___CU___________UCG_UUUUUUGUCUUCAGA___________GC______GC__UCUU__C__AUAAUC___CCUGUUUCU__UUCU_UAUUUU______________ACGCUUG\n______________|||_|||__|:|||||:|||||_____||:_||||||___|:___________||:_|||:|::||||||:|___________||______||__||||__|__|:||||___||::|:|||__|||:_:||||:______________||||||:\n______________GAG_AGU__UUGUCCUGUAACU_____AGG_GUCGUU___GG___________AGU_AAAGAGUAGAAGUUU___________CG______CG__AGAA__G__UGUUAG___GGGUAGAGA__AAGG_GUAAAG______________UGCGAAU\n3'-AUU...AAAAU___A___AG_____________GUAAC___C______CAA__UAUAUUACAAC___G_______________CAUUACUUAC___AAGCAA__UU____CC_UU______CAU_________AU____G______AAACUUUAAACUUU_______UAAAG...CAA-5'\n_____________|____________________________________________________________________________________________________________________________________________________________|\n_____________168__________________________________________________________________________________________________________________________________________________________33\n |
142 -- 148 |
13 -- 19 |
-70.4657 kcal/mol |
17.0109 kcal/mol |
33.4152 kcal/mol |
25 135@LB@ | |@LB@5'-UUU...AACAU A AAA AUA A AGCAAAAAAAC C C UC C CU A AUCAAUC CAUGC...CGC-3'@LB@ CUC UCA AGCAGGAUAUUGA UCU CAGCAA CU UCG UUUUUUGUCUUCAGA GC GC UCUU C AUAAUC CCUGUUUCU UUCU UAUUUU ACGCUUG@LB@ ||| ||| |:|||||:||||| ||: |||||| |: ||: |||:|::||||||:| || || |||| | |:|||| ||::|:||| |||: :||||: ||||||:@LB@ GAG AGU UUGUCCUGUAACU AGG GUCGUU GG AGU AAAGAGUAGAAGUUU CG CG AGAA G UGUUAG GGGUAGAGA AAGG GUAAAG UGCGAAU@LB@3'-AUU...AAAAU A AG GUAAC C CAA UAUAUUACAAC G CAUUACUUAC AAGCAA UU CC UU CAU AU G AAACUUUAAACUUU UAAAG...CAA-5'@LB@ | |@LB@ 168 33@LB@@LB@@LB@Energy -23.88 kcal/mol@LB@Hybridization Energy -70.4657 kcal/mol@LB@Unfolding Energy - mRNA 17.0109 kcal/mol@LB@Unfolding Energy - sRNA 33.4152 kcal/mol@LB@@LB@Position - mRNA 26 -- 134@LB@Position - sRNA 34 -- 167@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 82 |
0.002803 |
0.1325 |
bsu34100 |
rsbQ |
-17.18 |
0.011511 |
5 -- 86 |
34 -- 116 |
sigma factor SigB regulation protein RsbQ |
|
_____________4__________________________________________________________________________________________87\n_____________|__________________________________________________________________________________________|\n_______5'-CAAA____________C__ACCCC_____________G__UAG______CUCCUCCU___UCUUC_____C____________CUA__U_____GUCCU...UGU-3'\n______________UGUCAUUUUUUU_UC_______________GGU_UC___GCAAUC________GCA_____CAAUU__AUCCAUCUUUU___UC_CCCAU\n______________|||||:|||:|:_||_______________||:_||___||||||________|||_____||||:__|:||||||:||___||_|||||\n______________ACAGUGAAAGAG_AG_______________CCG_AG___CGUUAG________CGU_____GUUAG__UGGGUAGAGAA___AG_GGGUA\n3'-AUU...UUACA____________U__AAGUUUCAUUACUUA___A__CAA______AAC________UU________CA___________UA_________AAGAA...CAA-5'\n_____________|__________________________________________________________________________________________|\n_____________117________________________________________________________________________________________33\n |
65 -- 71 |
47 -- 53 |
-43.7075 kcal/mol |
8.64393 kcal/mol |
22.4324 kcal/mol |
4 87@LB@ | |@LB@ 5'-CAAA C ACCCC G UAG CUCCUCCU UCUUC C CUA U GUCCU...UGU-3'@LB@ UGUCAUUUUUUU UC GGU UC GCAAUC GCA CAAUU AUCCAUCUUUU UC CCCAU@LB@ |||||:|||:|: || ||: || |||||| ||| ||||: |:||||||:|| || |||||@LB@ ACAGUGAAAGAG AG CCG AG CGUUAG CGU GUUAG UGGGUAGAGAA AG GGGUA@LB@3'-AUU...UUACA U AAGUUUCAUUACUUA A CAA AAC UU CA UA AAGAA...CAA-5'@LB@ | |@LB@ 117 33@LB@@LB@@LB@Energy -17.18 kcal/mol@LB@Hybridization Energy -43.7075 kcal/mol@LB@Unfolding Energy - mRNA 8.64393 kcal/mol@LB@Unfolding Energy - sRNA 22.4324 kcal/mol@LB@@LB@Position - mRNA 5 -- 86@LB@Position - sRNA 34 -- 116@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 7 |
0.0001858 |
0.08823 |
bsu34210 |
yvfG |
-17.07 |
0.012126 |
85 -- 142 |
32 -- 86 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________84____________________________________________________________143\n_____________|_____________________________________________________________|\n5'-GAG...GUUGA______AAUAC___________C____GUU__A__________GCUCAC____________CAUUG...ACU-3'\n______________GGCUUU_____UUGUA__CUUG_UGAA___UC_UAUCCAUCUU______UCCCC_UUUCUU\n______________|||||:_____|||:|__||||_::||___||_||:||||||:______|||||_||||||\n______________CCGAAG_____AACGU__GAAC_GUUU___AG_AUGGGUAGAG______AGGGG_AAAGAA\n3'-AUU...ACUUA______C_________UA____C____GUU__C__________AAUA_______U______ACUUU...CAA-5'\n_____________|_____________________________________________________________|\n_____________87____________________________________________________________31\n |
119 -- 125 |
48 -- 54 |
-40.7387 kcal/mol |
11.2598 kcal/mol |
14.8836 kcal/mol |
84 143@LB@ | |@LB@5'-GAG...GUUGA AAUAC C GUU A GCUCAC CAUUG...ACU-3'@LB@ GGCUUU UUGUA CUUG UGAA UC UAUCCAUCUU UCCCC UUUCUU@LB@ |||||: |||:| |||| ::|| || ||:||||||: ||||| ||||||@LB@ CCGAAG AACGU GAAC GUUU AG AUGGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACUUA C UA C GUU C AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 87 31@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -40.7387 kcal/mol@LB@Unfolding Energy - mRNA 11.2598 kcal/mol@LB@Unfolding Energy - sRNA 14.8836 kcal/mol@LB@@LB@Position - mRNA 85 -- 142@LB@Position - sRNA 32 -- 86@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 88 |
0.003231 |
0.1423 |
bsu34620 |
mdxD |
-9.76 |
0.344052 |
128 -- 173 |
81 -- 130 |
glucan 1 4-alpha-maltohexaosidase |
|
_____________127____________174\n_____________|______________|\n5'-GUU...AUGCA__GA_______C__GCCAU...GUG-3'\n______________GC__UUCAUCA_CA\n______________:|__|||||||_||\n______________UG__AAGUAGU_GU\n3'-AUU...AAAAA__AG_______U__CCUGU...CAA-5'\n_____________|______________|\n_____________131____________80\n |
220 -- 226 |
158 -- 164 |
-10.4913 kcal/mol |
1.73429 kcal/mol |
1.41528 kcal/mol |
127 174@LB@ | |@LB@5'-GUU...AUGCA GA C GCCAU...GUG-3'@LB@ GC UUCAUCA CA@LB@ :| ||||||| ||@LB@ UG AAGUAGU GU@LB@3'-AUU...AAAAA AG U CCUGU...CAA-5'@LB@ | |@LB@ 131 80@LB@@LB@@LB@Energy -9.76 kcal/mol@LB@Hybridization Energy -10.4913 kcal/mol@LB@Unfolding Energy - mRNA 1.73429 kcal/mol@LB@Unfolding Energy - sRNA 1.41528 kcal/mol@LB@@LB@Position - mRNA 128 -- 173@LB@Position - sRNA 81 -- 130@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 63 |
0.001974 |
0.1214 |
bsu34640 |
yvdD |
-9.92 |
0.323853 |
41 -- 65 |
33 -- 60 |
LOG family protein YvdD |
|
_____________40___________________________66\n_____________|____________________________|\n5'-GAC...GUAAA______UCAUCCAUUUUUCUG_______CAGCU...GAA-3'\n______________ACUCUU_______________CAUCAGC\n______________||||||_______________|||||:|\n______________UGAGAA_______________GUAGUUG\n3'-AUU...AAAAA____________________________UCCUG...CAA-5'\n_____________|____________________________|\n_____________61___________________________32\n |
69 -- 75 |
156 -- 162 |
-11.7297 kcal/mol |
2.56294 kcal/mol |
1.40592 kcal/mol |
40 66@LB@ | |@LB@5'-GAC...GUAAA UCAUCCAUUUUUCUG CAGCU...GAA-3'@LB@ ACUCUU CAUCAGC@LB@ |||||| |||||:|@LB@ UGAGAA GUAGUUG@LB@3'-AUU...AAAAA UCCUG...CAA-5'@LB@ | |@LB@ 61 32@LB@@LB@@LB@Energy -9.92 kcal/mol@LB@Hybridization Energy -11.7297 kcal/mol@LB@Unfolding Energy - mRNA 2.56294 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 41 -- 65@LB@Position - sRNA 33 -- 60@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 16 |
0.0004911 |
0.1011 |
bsu34650 |
yvdC |
-21.31 |
0.001462 |
17 -- 94 |
101 -- 185 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pe: enzyme' |
|
_____________16_______________________________________________________________________95\n_____________|________________________________________________________________________|\n5'-AUA...UUUUA___G_______UCCCC_____U______C_________AAUACA____A________U____C__G______CCGCA...AGG-3'\n______________UAC_CUUCAUU_____CAGGG__UUGAU_____CCGCA________CA_AUG__GUU_UCAU_UU_UCAUCU\n______________|||_||||||:_____||||:__||||:_____|||||________||_||:__|||_|||:_||_||||||\n______________AUG_GAAGUAG_____GUCCU__AACUG_____GGCGU________GU_UAU__CAA_AGUG_AA_AGUAGA\n3'-AUU...AAAAA___A_______UU________GU_____UAACA_____CGUUCAAG__A___UA___C____A__G______AGUUU...CAA-5'\n_____________|________________________________________________________________________|\n_____________186______________________________________________________________________100\n |
36 -- 42 |
159 -- 165 |
-36.2616 kcal/mol |
8.0324 kcal/mol |
15.7762 kcal/mol |
16 95@LB@ | |@LB@5'-AUA...UUUUA G UCCCC U C AAUACA A U C G CCGCA...AGG-3'@LB@ UAC CUUCAUU CAGGG UUGAU CCGCA CA AUG GUU UCAU UU UCAUCU@LB@ ||| ||||||: ||||: ||||: ||||| || ||: ||| |||: || ||||||@LB@ AUG GAAGUAG GUCCU AACUG GGCGU GU UAU CAA AGUG AA AGUAGA@LB@3'-AUU...AAAAA A UU GU UAACA CGUUCAAG A UA C A G AGUUU...CAA-5'@LB@ | |@LB@ 186 100@LB@@LB@@LB@Energy -21.31 kcal/mol@LB@Hybridization Energy -36.2616 kcal/mol@LB@Unfolding Energy - mRNA 8.0324 kcal/mol@LB@Unfolding Energy - sRNA 15.7762 kcal/mol@LB@@LB@Position - mRNA 17 -- 94@LB@Position - sRNA 101 -- 185@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 159 -- 165@LB@ |
| 29 |
0.000814 |
0.1059 |
bsu35860 |
pgdS |
-7.84 |
0.648005 |
23 -- 34 |
130 -- 140 |
gamma-dl-glutamyl hydrolase |
|
______________23________________________________35\n______________|_________________________________|\n___________5'-________GGAAAUGAAUAAAA___A________AAGAA...AUA-3'\n______________AUUGAUAU______________UCC_GCAGCAGG\n______________|||||:||______________|||_||||||:|\n______________UAACUGUA______________AGG_CGUCGUUC\n3'-AUU...UCCUG________AC________________________AAGGU...CAA-5'\n_____________|__________________________________|\n_____________141________________________________129\n |
28 -- 34 |
130 -- 136 |
-20.8842 kcal/mol |
7.79874 kcal/mol |
6.50607 kcal/mol |
23 35@LB@ | |@LB@ 5'- GGAAAUGAAUAAAA A AAGAA...AUA-3'@LB@ AUUGAUAU UCC GCAGCAGG@LB@ |||||:|| ||| ||||||:|@LB@ UAACUGUA AGG CGUCGUUC@LB@3'-AUU...UCCUG AC AAGGU...CAA-5'@LB@ | |@LB@ 141 129@LB@@LB@@LB@Energy -7.84 kcal/mol@LB@Hybridization Energy -20.8842 kcal/mol@LB@Unfolding Energy - mRNA 7.79874 kcal/mol@LB@Unfolding Energy - sRNA 6.50607 kcal/mol@LB@@LB@Position - mRNA 23 -- 34@LB@Position - sRNA 130 -- 140@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 130 -- 136@LB@ |
