| 91 |
0.00349 |
0.1486 |
bsu28050 |
maf |
-6.37 |
0.874324 |
134 -- 184 |
39 -- 85 |
septum formation protein Maf |
|
_____________133_____185\n_____________|_______|\n5'-GAA...CCCUA_______AUUGU...AAG-3'\n______________CUCCAUU\n______________|:|||||\n______________GGGGUAA\n3'-AUU...AAUAA_______AGAAA...CAA-5'\n_____________|_______|\n_____________86______38\n |
275 -- 281 |
36 -- 42 |
-8.4297 kcal/mol |
0.414575 kcal/mol |
2.55634 kcal/mol |
133 185@LB@ | |@LB@5'-GAA...CCCUA AUUGU...AAG-3'@LB@ CUCCAUU@LB@ |:|||||@LB@ GGGGUAA@LB@3'-AUU...AAUAA AGAAA...CAA-5'@LB@ | |@LB@ 86 38@LB@@LB@@LB@Energy -6.37 kcal/mol@LB@Hybridization Energy -8.4297 kcal/mol@LB@Unfolding Energy - mRNA 0.414575 kcal/mol@LB@Unfolding Energy - sRNA 2.55634 kcal/mol@LB@@LB@Position - mRNA 134 -- 184@LB@Position - sRNA 39 -- 85@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 66 |
0.002095 |
0.123 |
bsu28660 |
sspI |
-12.58 |
0.103691 |
257 -- 278 |
56 -- 77 |
small acid-soluble spore protein I |
|
_____________256_____________________279\n_____________|_______________________|\n5'-UUG...CAGGA_________A___U_________GAUGC...AAG-3'\n______________GCAG_CUUG_GCA_ACAAUCGUA\n______________|||:_||||_|||_|||||||||\n______________CGUU_GAAC_CGU_UGUUAGCAU\n3'-AUU...AGCAA____A________U_________GGGUA...CAA-5'\n_____________|_______________________|\n_____________78______________________55\n |
271 -- 277 |
57 -- 63 |
-25.139 kcal/mol |
6.36558 kcal/mol |
6.79705 kcal/mol |
256 279@LB@ | |@LB@5'-UUG...CAGGA A U GAUGC...AAG-3'@LB@ GCAG CUUG GCA ACAAUCGUA@LB@ |||: |||| ||| |||||||||@LB@ CGUU GAAC CGU UGUUAGCAU@LB@3'-AUU...AGCAA A U GGGUA...CAA-5'@LB@ | |@LB@ 78 55@LB@@LB@@LB@Energy -12.58 kcal/mol@LB@Hybridization Energy -25.139 kcal/mol@LB@Unfolding Energy - mRNA 6.36558 kcal/mol@LB@Unfolding Energy - sRNA 6.79705 kcal/mol@LB@@LB@Position - mRNA 257 -- 278@LB@Position - sRNA 56 -- 77@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 57 -- 63@LB@ |
| 149 |
0.006788 |
0.1741 |
bsu28960 |
ytxC |
-13.61 |
0.064351 |
236 -- 300 |
132 -- 186 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________235________________________301\n_____________|__________________________|\n________5'-CGA__U________U______________CUUUC...GCA-3'\n______________GC__UUUUGGC_GGCAAUC_UGCCCG\n______________||__|:|||||_::|||||_|:|||:\n______________CG__AGAACCG_UUGUUAG_AUGGGU\n3'-AUU...AGCAA__UU_______U_______C______AGAGA...CAA-5'\n_____________|__________________________|\n_____________187________________________131\n |
7 -- 13 |
67 -- 73 |
-27.0931 kcal/mol |
7.22978 kcal/mol |
8.4393 kcal/mol |
235 301@LB@ | |@LB@ 5'-CGA U U CUUUC...GCA-3'@LB@ GC UUUUGGC GGCAAUC UGCCCG@LB@ || |:||||| ::||||| |:|||:@LB@ CG AGAACCG UUGUUAG AUGGGU@LB@3'-AUU...AGCAA UU U C AGAGA...CAA-5'@LB@ | |@LB@ 187 131@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -27.0931 kcal/mol@LB@Unfolding Energy - mRNA 7.22978 kcal/mol@LB@Unfolding Energy - sRNA 8.4393 kcal/mol@LB@@LB@Position - mRNA 236 -- 300@LB@Position - sRNA 132 -- 186@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 140 |
0.006451 |
0.1741 |
bsu29090 |
polA |
-11.51 |
0.167440 |
58 -- 84 |
30 -- 56 |
DNA polymerase I |
|
_____________57________________85\n_____________|_________________|\n5'-AUG...CGGGA________G________GUCCU...AUA-3'\n______________ACCUAUUU__UGUUCCC\n______________|||:||:|__|:|||||\n______________UGGGUAGA__AUAAGGG\n3'-AUU...UAGCA________GA_______GUAAA...CAA-5'\n_____________|_________________|\n_____________57________________29\n |
67 -- 73 |
40 -- 46 |
-17.5976 kcal/mol |
2.41953 kcal/mol |
7.22212 kcal/mol |
57 85@LB@ | |@LB@5'-AUG...CGGGA G GUCCU...AUA-3'@LB@ ACCUAUUU UGUUCCC@LB@ |||:||:| |:|||||@LB@ UGGGUAGA AUAAGGG@LB@3'-AUU...UAGCA GA GUAAA...CAA-5'@LB@ | |@LB@ 57 29@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -17.5976 kcal/mol@LB@Unfolding Energy - mRNA 2.41953 kcal/mol@LB@Unfolding Energy - sRNA 7.22212 kcal/mol@LB@@LB@Position - mRNA 58 -- 84@LB@Position - sRNA 30 -- 56@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 52 |
0.001732 |
0.1212 |
bsu29530 |
sppA |
-10.15 |
0.295539 |
2 -- 29 |
39 -- 81 |
signal peptide peptidase SppA |
|
_____________1________________________________________________________30\n_____________|________________________________________________________|\n5'-GCG...CUCUG_G______C____UGUCUAUU_____CAG__________GA_______________GCGUC...AAG-3'\n______________G_GAUUUU_GGCG________AUCGU___CAUCUC_UAU_____GUUUCUUUGAAA\n______________|_:||:||_|||:________|||||___||||||_|||_____:|||||||||||\n______________C_UUAGAA_CCGU________UAGCA___GUAGAG_AUA_____UAAAGAAACUUU\n3'-AUU...AGCAA_G___________UUGU_________UGG______A___AGGGG____________AAACU...CAA-5'\n_____________|________________________________________________________|\n_____________82_______________________________________________________38\n |
284 -- 290 |
28 -- 34 |
-34.8894 kcal/mol |
12.7856 kcal/mol |
14.4529 kcal/mol |
1 30@LB@ | |@LB@5'-GCG...CUCUG G C UGUCUAUU CAG GA GCGUC...AAG-3'@LB@ G GAUUUU GGCG AUCGU CAUCUC UAU GUUUCUUUGAAA@LB@ | :||:|| |||: ||||| |||||| ||| :|||||||||||@LB@ C UUAGAA CCGU UAGCA GUAGAG AUA UAAAGAAACUUU@LB@3'-AUU...AGCAA G UUGU UGG A AGGGG AAACU...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -10.15 kcal/mol@LB@Hybridization Energy -34.8894 kcal/mol@LB@Unfolding Energy - mRNA 12.7856 kcal/mol@LB@Unfolding Energy - sRNA 14.4529 kcal/mol@LB@@LB@Position - mRNA 2 -- 29@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 28 -- 34@LB@ |
| 146 |
0.00677 |
0.1741 |
bsu29690 |
acuA |
-9.85 |
0.331997 |
126 -- 137 |
14 -- 25 |
acetoin utilization protein AcuA |
|
_____________125____________138\n_____________|______________|\n5'-UAU...AUUCA______________UUUUA...AUG-3'\n______________UAUUCUUC_UCAAU\n______________||:|||||_||||:\n______________AUGAGAAG_AGUUG\n3'-AUU...AAAAA________U_____UCCUG...CAA-5'\n_____________|______________|\n_____________26_____________13\n |
95 -- 101 |
162 -- 168 |
-12.3213 kcal/mol |
1.49895 kcal/mol |
1.40683 kcal/mol |
125 138@LB@ | |@LB@5'-UAU...AUUCA UUUUA...AUG-3'@LB@ UAUUCUUC UCAAU@LB@ ||:||||| ||||:@LB@ AUGAGAAG AGUUG@LB@3'-AUU...AAAAA U UCCUG...CAA-5'@LB@ | |@LB@ 26 13@LB@@LB@@LB@Energy -9.85 kcal/mol@LB@Hybridization Energy -12.3213 kcal/mol@LB@Unfolding Energy - mRNA 1.49895 kcal/mol@LB@Unfolding Energy - sRNA 1.40683 kcal/mol@LB@@LB@Position - mRNA 126 -- 137@LB@Position - sRNA 14 -- 25@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 162 -- 168@LB@ |
| 51 |
0.001644 |
0.1212 |
bsu29870 |
ytzB |
-11.72 |
0.152375 |
23 -- 59 |
33 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________22__________________________________________60\n_____________|___________________________________________|\n5'-CUG...AUUGC___________________________________________AAAAG...AAG-3'\n______________UUAACAGGAU_UUGGC__UGUU__________UCUGUAUAAUG\n______________|:|||||||:_|||:|__|||:__________||::|||||||\n______________AGUUGUCCUG_AACUG__ACAG__________AGGUAUAUUAC\n3'-AUU...GAAGU__________U_____UA____GCGUCGUUCA___________AACAG...CAA-5'\n_____________|___________________________________________|\n_____________86__________________________________________32\n |
163 -- 169 |
118 -- 124 |
-27.3559 kcal/mol |
7.06665 kcal/mol |
10.6143 kcal/mol |
22 60@LB@ | |@LB@5'-CUG...AUUGC AAAAG...AAG-3'@LB@ UUAACAGGAU UUGGC UGUU UCUGUAUAAUG@LB@ |:|||||||: |||:| |||: ||::|||||||@LB@ AGUUGUCCUG AACUG ACAG AGGUAUAUUAC@LB@3'-AUU...GAAGU U UA GCGUCGUUCA AACAG...CAA-5'@LB@ | |@LB@ 86 32@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -27.3559 kcal/mol@LB@Unfolding Energy - mRNA 7.06665 kcal/mol@LB@Unfolding Energy - sRNA 10.6143 kcal/mol@LB@@LB@Position - mRNA 23 -- 59@LB@Position - sRNA 33 -- 85@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 98 |
0.003906 |
0.1544 |
bsu29990 |
pbuO |
-11.33 |
0.180719 |
218 -- 233 |
123 -- 137 |
guanine/hypoxanthine permease PbuO |
|
_____________217______________234\n_____________|________________|\n5'-GGU...AAAGA_______ACA______AAGCA...CGG-3'\n______________GCAGCAA___UCCAUA\n______________|||||||___||||||\n______________CGUCGUU___AGGUAU\n3'-AUU...ACAGG_______CA_______AUUAC...CAA-5'\n_____________|________________|\n_____________138______________122\n |
218 -- 224 |
131 -- 137 |
-16.3971 kcal/mol |
2.46726 kcal/mol |
3.21202 kcal/mol |
217 234@LB@ | |@LB@5'-GGU...AAAGA ACA AAGCA...CGG-3'@LB@ GCAGCAA UCCAUA@LB@ ||||||| ||||||@LB@ CGUCGUU AGGUAU@LB@3'-AUU...ACAGG CA AUUAC...CAA-5'@LB@ | |@LB@ 138 122@LB@@LB@@LB@Energy -11.33 kcal/mol@LB@Hybridization Energy -16.3971 kcal/mol@LB@Unfolding Energy - mRNA 2.46726 kcal/mol@LB@Unfolding Energy - sRNA 3.21202 kcal/mol@LB@@LB@Position - mRNA 218 -- 233@LB@Position - sRNA 123 -- 137@LB@Position Seed - mRNA 218 -- 224@LB@Position Seed - sRNA 131 -- 137@LB@ |
| 139 |
0.006395 |
0.1741 |
bsu30170 |
ytcP |
-13.74 |
0.060669 |
6 -- 28 |
1 -- 23 |
ABC transporter permease |
|
_____________5_______________________29\n_____________|_______________________|\n______5'-UCUAA__UU_____CUU___________AUCGG...UUG-3'\n______________GA__AUGCU___UUUCUUUUGUU\n______________||__|:|||___|||||||||||\n______________CU__UGCGA___AAAGAAAACAA\n3'-AUU...UUAAA__UU_____AUU___________-5'\n_____________|______________________|\n_____________24_____________________1\n |
22 -- 28 |
1 -- 7 |
-18.7227 kcal/mol |
2.34195 kcal/mol |
4.12892 kcal/mol |
5 29@LB@ | |@LB@ 5'-UCUAA UU CUU AUCGG...UUG-3'@LB@ GA AUGCU UUUCUUUUGUU@LB@ || |:||| |||||||||||@LB@ CU UGCGA AAAGAAAACAA@LB@3'-AUU...UUAAA UU AUU -5'@LB@ | |@LB@ 24 1@LB@@LB@@LB@Energy -13.74 kcal/mol@LB@Hybridization Energy -18.7227 kcal/mol@LB@Unfolding Energy - mRNA 2.34195 kcal/mol@LB@Unfolding Energy - sRNA 4.12892 kcal/mol@LB@@LB@Position - mRNA 6 -- 28@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 1 -- 7@LB@ |
| 55 |
0.001813 |
0.1212 |
bsu30290 |
amyC |
-12.90 |
0.089506 |
91 -- 153 |
102 -- 159 |
ABC transporter permease |
|
_____________90_________________________________________________________________154\n_____________|__________________________________________________________________|\n5'-CUG...UCAAA_________GCC___UA________AC_____G_______UU___GCAU__________GA_____AGCUU...CUU-3'\n______________CGGCAGGAU___GGC__UG_CCGCA__GCAAG___CAUAU__AUG____UUGUCAUUUU__UCAUC\n______________|::|||||:___|:|__||_|||||__|||||___|||||__|||____||||||:|||__|||||\n______________GUUGUCCUG___CUG__AC_GGCGU__CGUUC___GUAUA__UAC____AACAGUGAAA__AGUAG\n3'-AUU...AAGUA_________UAA___UA__A____________AAG_____U__________________G______AAGUU...CAA-5'\n_____________|__________________________________________________________________|\n_____________160________________________________________________________________101\n |
139 -- 145 |
109 -- 115 |
-48.9264 kcal/mol |
23.4207 kcal/mol |
14.7573 kcal/mol |
90 154@LB@ | |@LB@5'-CUG...UCAAA GCC UA AC G UU GCAU GA AGCUU...CUU-3'@LB@ CGGCAGGAU GGC UG CCGCA GCAAG CAUAU AUG UUGUCAUUUU UCAUC@LB@ |::|||||: |:| || ||||| ||||| ||||| ||| ||||||:||| |||||@LB@ GUUGUCCUG CUG AC GGCGU CGUUC GUAUA UAC AACAGUGAAA AGUAG@LB@3'-AUU...AAGUA UAA UA A AAG U G AAGUU...CAA-5'@LB@ | |@LB@ 160 101@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -48.9264 kcal/mol@LB@Unfolding Energy - mRNA 23.4207 kcal/mol@LB@Unfolding Energy - sRNA 14.7573 kcal/mol@LB@@LB@Position - mRNA 91 -- 153@LB@Position - sRNA 102 -- 159@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 109 -- 115@LB@ |
