| 46 |
0.001434 |
0.1206 |
bsu07700 |
nagP |
-14.91 |
0.034715 |
11 -- 49 |
31 -- 62 |
PTS system-N-acetylglucosamine-specific transporter subunit EIICB |
|
_____________10______________________________________50\n_____________|_______________________________________|\n5'-UCU...UUCUU______UUGUUACAA______G_C______GC_______UUAAA...UAU-3'\n______________GAUCGU_________UCAUCU_U_AUUCCC__UUUCUUU\n______________:|||||_________:|||||_|_||||||__|||||||\n______________UUAGCA_________GGUAGA_A_UAAGGG__AAAGAAA\n3'-AUU...GUUUG______UG_____________G_A______GU_______CUUUA...CAA-5'\n_____________|_______________________________________|\n_____________63______________________________________30\n |
43 -- 49 |
31 -- 37 |
-26.4462 kcal/mol |
2.72756 kcal/mol |
10.7322 kcal/mol |
10 50@LB@ | |@LB@5'-UCU...UUCUU UUGUUACAA G C GC UUAAA...UAU-3'@LB@ GAUCGU UCAUCU U AUUCCC UUUCUUU@LB@ :||||| :||||| | |||||| |||||||@LB@ UUAGCA GGUAGA A UAAGGG AAAGAAA@LB@3'-AUU...GUUUG UG G A GU CUUUA...CAA-5'@LB@ | |@LB@ 63 30@LB@@LB@@LB@Energy -14.91 kcal/mol@LB@Hybridization Energy -26.4462 kcal/mol@LB@Unfolding Energy - mRNA 2.72756 kcal/mol@LB@Unfolding Energy - sRNA 10.7322 kcal/mol@LB@@LB@Position - mRNA 11 -- 49@LB@Position - sRNA 31 -- 62@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 154 |
0.00707 |
0.1751 |
bsu07830 |
yfkO |
-12.90 |
0.089530 |
138 -- 152 |
158 -- 172 |
NAD(P)H nitroreductase YfkO |
|
_____________137____________153\n_____________|______________|\n5'-GGA...GUACU______________UAAUG...AAU-3'\n______________UUUACUUUUUAUCA\n______________||||||:||:||||\n______________AAAUGAGAAGUAGU\n3'-AUU...AAAAA______________UGUCC...CAA-5'\n_____________|______________|\n_____________173____________157\n |
146 -- 152 |
158 -- 164 |
-13.59 kcal/mol |
1.38023 kcal/mol |
1.02713 kcal/mol |
137 153@LB@ | |@LB@5'-GGA...GUACU UAAUG...AAU-3'@LB@ UUUACUUUUUAUCA@LB@ ||||||:||:||||@LB@ AAAUGAGAAGUAGU@LB@3'-AUU...AAAAA UGUCC...CAA-5'@LB@ | |@LB@ 173 157@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -13.59 kcal/mol@LB@Unfolding Energy - mRNA 1.38023 kcal/mol@LB@Unfolding Energy - sRNA 1.02713 kcal/mol@LB@@LB@Position - mRNA 138 -- 152@LB@Position - sRNA 158 -- 172@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 76 |
0.002466 |
0.1257 |
bsu07870 |
yfkK |
-15.12 |
0.031390 |
12 -- 33 |
33 -- 55 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________11______________________34\n_____________|_______________________|\n5'-UGU...ACCUC__________UGC__________CUAAA...UCA-3'\n______________CCUA_UUCUU___CCCUAUUUCU\n______________||:|_:||||___|||:||||||\n______________GGGU_GAGAA___GGGGUAAAGA\n3'-AUU...AGCAU____A_____UAA__________AACUU...CAA-5'\n_____________|_______________________|\n_____________56______________________32\n |
27 -- 33 |
33 -- 39 |
-21.8682 kcal/mol |
0.689294 kcal/mol |
9.04338 kcal/mol |
11 34@LB@ | |@LB@5'-UGU...ACCUC UGC CUAAA...UCA-3'@LB@ CCUA UUCUU CCCUAUUUCU@LB@ ||:| :|||| |||:||||||@LB@ GGGU GAGAA GGGGUAAAGA@LB@3'-AUU...AGCAU A UAA AACUU...CAA-5'@LB@ | |@LB@ 56 32@LB@@LB@@LB@Energy -15.12 kcal/mol@LB@Hybridization Energy -21.8682 kcal/mol@LB@Unfolding Energy - mRNA 0.689294 kcal/mol@LB@Unfolding Energy - sRNA 9.04338 kcal/mol@LB@@LB@Position - mRNA 12 -- 33@LB@Position - sRNA 33 -- 55@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 108 |
0.004583 |
0.1633 |
bsu07910 |
yfkF |
-13.96 |
0.054522 |
60 -- 82 |
157 -- 179 |
MFS transporter |
|
_____________59______________83\n_____________|_______________|\n5'-GGC...UUAUU_______________AAACC...CAA-3'\n______________UUUAUUCUUCA_CAA\n______________||||:||||||_|||\n______________AAAUGAGAAGU_GUU\n3'-AUU...AAAAA___________A___GUCCU...CAA-5'\n_____________|_______________|\n_____________180_____________156\n |
73 -- 79 |
161 -- 167 |
-12.117 kcal/mol |
0.967911 kcal/mol |
1.36352 kcal/mol |
59 83@LB@ | |@LB@5'-GGC...UUAUU AAACC...CAA-3'@LB@ UUUAUUCUUCA CAA@LB@ ||||:|||||| |||@LB@ AAAUGAGAAGU GUU@LB@3'-AUU...AAAAA A GUCCU...CAA-5'@LB@ | |@LB@ 180 156@LB@@LB@@LB@Energy -13.96 kcal/mol@LB@Hybridization Energy -12.117 kcal/mol@LB@Unfolding Energy - mRNA 0.967911 kcal/mol@LB@Unfolding Energy - sRNA 1.36352 kcal/mol@LB@@LB@Position - mRNA 60 -- 82@LB@Position - sRNA 157 -- 179@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 161 -- 167@LB@ |
| 25 |
0.0006626 |
0.1027 |
bsu07955 |
yfkA |
-14.87 |
0.035327 |
3 -- 70 |
36 -- 108 |
radical SAM protein YfkA |
|
_____________2______________71\n_____________|______________|\n5'-AGU...GCUAC______________AAUCA...ACG-3'\n______________UUGCGAUCUUGGCA\n______________||||:|||||||||\n______________AACGUUAGAACCGU\n3'-AUU...GAAGC______________UUGUU...CAA-5'\n_____________|______________|\n_____________109____________35\n |
130 -- 136 |
66 -- 72 |
-22.3996 kcal/mol |
5.8798 kcal/mol |
4.6863 kcal/mol |
2 71@LB@ | |@LB@5'-AGU...GCUAC AAUCA...ACG-3'@LB@ UUGCGAUCUUGGCA@LB@ ||||:|||||||||@LB@ AACGUUAGAACCGU@LB@3'-AUU...GAAGC UUGUU...CAA-5'@LB@ | |@LB@ 109 35@LB@@LB@@LB@Energy -14.87 kcal/mol@LB@Hybridization Energy -22.3996 kcal/mol@LB@Unfolding Energy - mRNA 5.8798 kcal/mol@LB@Unfolding Energy - sRNA 4.6863 kcal/mol@LB@@LB@Position - mRNA 3 -- 70@LB@Position - sRNA 36 -- 108@LB@Position Seed - mRNA 130 -- 136@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 104 |
0.004338 |
0.1606 |
bsu08000 |
yfjQ |
-9.77 |
0.342120 |
136 -- 162 |
103 -- 137 |
metal ion transporter YfjQ |
bsu24740 |
_____________135_______________163\n_____________|_________________|\n5'-CAU...AUUAG____A____A_______CUUUU...AUU-3'\n______________GCAG_GAGU_CCGUAUA\n______________||||_:|||_||:||||\n______________CGUC_UUCA_GGUAUAU\n3'-AUU...ACAGG____G____A_______UACAA...CAA-5'\n_____________|_________________|\n_____________138_______________102\n |
146 -- 152 |
121 -- 127 |
-16.4629 kcal/mol |
4.99509 kcal/mol |
3.33887 kcal/mol |
135 163@LB@ | |@LB@5'-CAU...AUUAG A A CUUUU...AUU-3'@LB@ GCAG GAGU CCGUAUA@LB@ |||| :||| ||:||||@LB@ CGUC UUCA GGUAUAU@LB@3'-AUU...ACAGG G A UACAA...CAA-5'@LB@ | |@LB@ 138 102@LB@@LB@@LB@Energy -9.77 kcal/mol@LB@Hybridization Energy -16.4629 kcal/mol@LB@Unfolding Energy - mRNA 4.99509 kcal/mol@LB@Unfolding Energy - sRNA 3.33887 kcal/mol@LB@@LB@Position - mRNA 136 -- 162@LB@Position - sRNA 103 -- 137@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 9 |
0.0002071 |
0.08823 |
bsu08425 |
mprF |
-16.98 |
0.012672 |
3 -- 50 |
34 -- 81 |
phosphatidylglycerol lysyltransferase |
|
_____________2_______________________________________________51\n_____________|_______________________________________________|\n_________5'-AC__________GU_______U__AU__________AUCAC________CAUUC...AUC-3'\n______________UGUUGU_AUC___GUAAAU_AU____UCCAUUUC_____UUCCUCAU\n______________:||||:_|||___|:|||:_||____:||||:||_____||||:|||\n______________GCAACG_UAG___CGUUUG_UA____GGGUAGAG_____AAGGGGUA\n3'-AUU...CCGAA______U___AAC______U__GCAU________AAU__________AAGAA...CAA-5'\n_____________|_______________________________________________|\n_____________82______________________________________________33\n |
39 -- 45 |
37 -- 43 |
-29.0114 kcal/mol |
1.80779 kcal/mol |
13.626 kcal/mol |
2 51@LB@ | |@LB@ 5'-AC GU U AU AUCAC CAUUC...AUC-3'@LB@ UGUUGU AUC GUAAAU AU UCCAUUUC UUCCUCAU@LB@ :||||: ||| |:|||: || :||||:|| ||||:|||@LB@ GCAACG UAG CGUUUG UA GGGUAGAG AAGGGGUA@LB@3'-AUU...CCGAA U AAC U GCAU AAU AAGAA...CAA-5'@LB@ | |@LB@ 82 33@LB@@LB@@LB@Energy -16.98 kcal/mol@LB@Hybridization Energy -29.0114 kcal/mol@LB@Unfolding Energy - mRNA 1.80779 kcal/mol@LB@Unfolding Energy - sRNA 13.626 kcal/mol@LB@@LB@Position - mRNA 3 -- 50@LB@Position - sRNA 34 -- 81@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 147 |
0.006774 |
0.1741 |
bsu08550 |
sspK |
-14.99 |
0.033457 |
165 -- 208 |
137 -- 183 |
small acid-soluble spore protein K |
|
_____________164_____________________________209\n_____________|_______________________________|\n5'-UUU...UUGAU___GAACC___G_____________G_____AAAUA...ACG-3'\n______________CUC_____UAG_GGGAUAU_GACAU_GUCCG\n______________|||_____:|:_:|||:||_|||||_|||||\n______________GAG_____GUU_UCCUGUA_CUGUA_CAGGC\n3'-AUU...AAAAU___AAGUA___G_______A_____A_____GUCGU...CAA-5'\n_____________|_______________________________|\n_____________184_____________________________136\n |
191 -- 197 |
149 -- 155 |
-28.8408 kcal/mol |
8.70873 kcal/mol |
7.49334 kcal/mol |
164 209@LB@ | |@LB@5'-UUU...UUGAU GAACC G G AAAUA...ACG-3'@LB@ CUC UAG GGGAUAU GACAU GUCCG@LB@ ||| :|: :|||:|| ||||| |||||@LB@ GAG GUU UCCUGUA CUGUA CAGGC@LB@3'-AUU...AAAAU AAGUA G A A GUCGU...CAA-5'@LB@ | |@LB@ 184 136@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -28.8408 kcal/mol@LB@Unfolding Energy - mRNA 8.70873 kcal/mol@LB@Unfolding Energy - sRNA 7.49334 kcal/mol@LB@@LB@Position - mRNA 165 -- 208@LB@Position - sRNA 137 -- 183@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 149 -- 155@LB@ |
| 184 |
0.008461 |
0.1751 |
bsu09120 |
dgcK |
-13.04 |
0.083992 |
38 -- 184 |
37 -- 168 |
diguanylate cyclase |
|
_____________37______________________________________185\n_____________|_______________________________________|\n5'-CAU...AAGUC___AA_______AAACUCUCCCUUCAA____________UACUA...GCA-3'\n______________UCC__AGCAGGU________________AUUUUCUCAUU\n______________|||__||||:||________________|:||||||||:\n______________AGG__UCGUUCA________________UGAAAGAGUAG\n3'-AUU...GUAAC___CG_______AGGUAUAUUACAACAG___________AAGUU...CAA-5'\n_____________|_______________________________________|\n_____________169_____________________________________36\n |
55 -- 61 |
102 -- 108 |
-22.9941 kcal/mol |
2.75938 kcal/mol |
10.7301 kcal/mol |
37 185@LB@ | |@LB@5'-CAU...AAGUC AA AAACUCUCCCUUCAA UACUA...GCA-3'@LB@ UCC AGCAGGU AUUUUCUCAUU@LB@ ||| ||||:|| |:||||||||:@LB@ AGG UCGUUCA UGAAAGAGUAG@LB@3'-AUU...GUAAC CG AGGUAUAUUACAACAG AAGUU...CAA-5'@LB@ | |@LB@ 169 36@LB@@LB@@LB@Energy -13.04 kcal/mol@LB@Hybridization Energy -22.9941 kcal/mol@LB@Unfolding Energy - mRNA 2.75938 kcal/mol@LB@Unfolding Energy - sRNA 10.7301 kcal/mol@LB@@LB@Position - mRNA 38 -- 184@LB@Position - sRNA 37 -- 168@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 32 |
0.0008744 |
0.1059 |
bsu09430 |
citR |
-15.39 |
0.027619 |
23 -- 103 |
39 -- 111 |
LysR family transcriptional regulator |
|
_____________22______________________________________________________________________________________104\n_____________|_______________________________________________________________________________________|\n5'-UGU...ACGCC________C_A____UAU__C______________UACACA_______CCCUU________CGUAAU__________G_________CUAUC...CCG-3'\n______________CUUUUUCA_C_UCAA___GG_UGAUGGAU_GUUUC______UGUAAUU________UAAUC______GUA_CCAUUU__UAUUCCCC\n______________||||:|||_|_||||___:|_|:|||:||_|:|||______||:|||:________:||||______|||_||||:|__||||||||\n______________GAAAGAGU_G_AGUU___UC_AUUACUUA_CGAAG______ACGUUAG________GUUAG______CAU_GGUAGA__AUAAGGGG\n3'-AUU...ACAGU________A_A__________________C_____CA___________AACCGUUU______________G______GA________UAAAG...CAA-5'\n_____________|_______________________________________________________________________________________|\n_____________112_____________________________________________________________________________________38\n |
97 -- 103 |
39 -- 45 |
-43.1846 kcal/mol |
11.0009 kcal/mol |
20.821 kcal/mol |
22 104@LB@ | |@LB@5'-UGU...ACGCC C A UAU C UACACA CCCUU CGUAAU G CUAUC...CCG-3'@LB@ CUUUUUCA C UCAA GG UGAUGGAU GUUUC UGUAAUU UAAUC GUA CCAUUU UAUUCCCC@LB@ ||||:||| | |||| :| |:|||:|| |:||| ||:|||: :|||| ||| ||||:| ||||||||@LB@ GAAAGAGU G AGUU UC AUUACUUA CGAAG ACGUUAG GUUAG CAU GGUAGA AUAAGGGG@LB@3'-AUU...ACAGU A A C CA AACCGUUU G GA UAAAG...CAA-5'@LB@ | |@LB@ 112 38@LB@@LB@@LB@Energy -15.39 kcal/mol@LB@Hybridization Energy -43.1846 kcal/mol@LB@Unfolding Energy - mRNA 11.0009 kcal/mol@LB@Unfolding Energy - sRNA 20.821 kcal/mol@LB@@LB@Position - mRNA 23 -- 103@LB@Position - sRNA 39 -- 111@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 39 -- 45@LB@ |
