| 188 |
0.008618 |
0.1751 |
bsu31720 |
degQ |
-9.47 |
0.382925 |
276 -- 300 |
147 -- 170 |
degradation enzyme regulation protein DegQ |
|
_____________275______________301\n_____________|________________|\n5'-GAA...AUUCA____GAAA________AAAGC...UUG-3'\n______________UUAC____CAUUAACA\n______________||||____|||:||||\n______________AAUG____GUAGUUGU\n3'-AUU...AAAAA____AGAA________CCUGU...CAA-5'\n_____________|________________|\n_____________171______________146\n |
285 -- 291 |
155 -- 161 |
-9.46814 kcal/mol |
0.995164 kcal/mol |
1.41808 kcal/mol |
275 301@LB@ | |@LB@5'-GAA...AUUCA GAAA AAAGC...UUG-3'@LB@ UUAC CAUUAACA@LB@ |||| |||:||||@LB@ AAUG GUAGUUGU@LB@3'-AUU...AAAAA AGAA CCUGU...CAA-5'@LB@ | |@LB@ 171 146@LB@@LB@@LB@Energy -9.47 kcal/mol@LB@Hybridization Energy -9.46814 kcal/mol@LB@Unfolding Energy - mRNA 0.995164 kcal/mol@LB@Unfolding Energy - sRNA 1.41808 kcal/mol@LB@@LB@Position - mRNA 276 -- 300@LB@Position - sRNA 147 -- 170@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 155 -- 161@LB@ |
| 86 |
0.003162 |
0.1423 |
bsu32080 |
yuiB |
-17.14 |
0.011766 |
214 -- 271 |
6 -- 63 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________213____________________________________________________________272\n_____________|______________________________________________________________|\n5'-UAG...UCUGC________A_______AA_______G_U____GUC___UUU_______U_____________AUUAA...GGA-3'\n______________CAGUCGUA_UCAUCUC__UUGUUUU_U_UUUU___UUG___UUUGGAA_CG____GUUUCUU\n______________||:|||||_:||||||__||:||::_:_|||:___|||___||||:||_||____:||||||\n______________GUUAGCAU_GGUAGAG__AAUAAGG_G_AAAG___AAC___AAACUUU_GC____UAAAGAA\n3'-AUU...CGUUU________G________________G_U____A_____UUU_______U__GAAU_______AACAA-5'\n_____________|______________________________________________________________|\n_____________64_____________________________________________________________5\n |
265 -- 271 |
6 -- 12 |
-35.4392 kcal/mol |
4.76592 kcal/mol |
13.9327 kcal/mol |
213 272@LB@ | |@LB@5'-UAG...UCUGC A AA G U GUC UUU U AUUAA...GGA-3'@LB@ CAGUCGUA UCAUCUC UUGUUUU U UUUU UUG UUUGGAA CG GUUUCUU@LB@ ||:||||| :|||||| ||:||:: : |||: ||| ||||:|| || :||||||@LB@ GUUAGCAU GGUAGAG AAUAAGG G AAAG AAC AAACUUU GC UAAAGAA@LB@3'-AUU...CGUUU G G U A UUU U GAAU AACAA-5'@LB@ | |@LB@ 64 5@LB@@LB@@LB@Energy -17.14 kcal/mol@LB@Hybridization Energy -35.4392 kcal/mol@LB@Unfolding Energy - mRNA 4.76592 kcal/mol@LB@Unfolding Energy - sRNA 13.9327 kcal/mol@LB@@LB@Position - mRNA 214 -- 271@LB@Position - sRNA 6 -- 63@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 6 -- 12@LB@ |
| 111 |
0.004819 |
0.1671 |
bsu32120 |
yuzG |
-7.22 |
0.752372 |
165 -- 182 |
149 -- 166 |
Evidence 5: No homology to any previously reported sequences |
|
_____________164______________________________________183\n_____________|________________________________________|\n5'-UAG...UCUUU_____A_G_ACA__G__AAG__A________GAUG_____AAACG...UGG-3'\n______________AUGGC_U_G___UG_GA____G_GCGAAUAA____GCCCA\n______________|||||_|_|___||_:|____|_||:||:||____:||||\n______________UACCG_A_C___AC_UU____C_CGUUUGUU____UGGGU\n3'-AUU...UUACU_____A_G_A____G__AGAA__________AGCA_____AGAGA...CAA-5'\n_____________|________________________________________|\n_____________167______________________________________148\n |
192 -- 198 |
62 -- 68 |
-24.2214 kcal/mol |
8.24153 kcal/mol |
10.1588 kcal/mol |
164 183@LB@ | |@LB@5'-UAG...UCUUU A G ACA G AAG A GAUG AAACG...UGG-3'@LB@ AUGGC U G UG GA G GCGAAUAA GCCCA@LB@ ||||| | | || :| | ||:||:|| :||||@LB@ UACCG A C AC UU C CGUUUGUU UGGGU@LB@3'-AUU...UUACU A G A G AGAA AGCA AGAGA...CAA-5'@LB@ | |@LB@ 167 148@LB@@LB@@LB@Energy -7.22 kcal/mol@LB@Hybridization Energy -24.2214 kcal/mol@LB@Unfolding Energy - mRNA 8.24153 kcal/mol@LB@Unfolding Energy - sRNA 10.1588 kcal/mol@LB@@LB@Position - mRNA 165 -- 182@LB@Position - sRNA 149 -- 166@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 168 |
0.007777 |
0.1751 |
bsu32270 |
yutH |
-11.70 |
0.153975 |
226 -- 259 |
121 -- 158 |
endospore coat-associated protein YutH |
|
_____________225____________________________________260\n_____________|______________________________________|\n5'-UGC...AGAAA____C______C____CAG_______U___________CCAGC...ACU-3'\n______________AAUA_GGCAUU_ACAU_____GCAGC___UUCCAUGUA\n______________||:|_|:||||_||||_____|||||___||||||:||\n______________UUGU_CUGUAA_UGUA_____CGUCG___AAGGUAUAU\n3'-AUU...AGUAG____C______C____ACAGG_____UUC_________UACAA...CAA-5'\n_____________|______________________________________|\n_____________159____________________________________120\n |
253 -- 259 |
121 -- 127 |
-28.4364 kcal/mol |
6.47482 kcal/mol |
10.499 kcal/mol |
225 260@LB@ | |@LB@5'-UGC...AGAAA C C CAG U CCAGC...ACU-3'@LB@ AAUA GGCAUU ACAU GCAGC UUCCAUGUA@LB@ ||:| |:|||| |||| ||||| ||||||:||@LB@ UUGU CUGUAA UGUA CGUCG AAGGUAUAU@LB@3'-AUU...AGUAG C C ACAGG UUC UACAA...CAA-5'@LB@ | |@LB@ 159 120@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -28.4364 kcal/mol@LB@Unfolding Energy - mRNA 6.47482 kcal/mol@LB@Unfolding Energy - sRNA 10.499 kcal/mol@LB@@LB@Position - mRNA 226 -- 259@LB@Position - sRNA 121 -- 158@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 97 |
0.003858 |
0.1541 |
bsu32520 |
pucG |
-15.24 |
0.029596 |
117 -- 232 |
36 -- 162 |
purine catabolism protein PucG |
|
_____________116_____________________________________________________________________________________________________________________________________233\n_____________|_______________________________________________________________________________________________________________________________________|\n5'-UGA...AUAUA_______CA____GC____G__CGGC__________CUGA__GAUUUAUUAUACAA___GG_______GA_____GAG_________GU_________A_______A__GAAU___G__________________AAGAA...CGG-3'\n______________CGUCAGC__GACA__UUGA_AU____GUCCGC_GCA____CU______________ACU__CUUA_CU__UAAAG____GAAUGGCU________GUC__GGCAGA_GA____UAU_CA_________CCCCGUU\n______________|:|||:|__||||__||||_||____||||||_|||____|:______________|||__||:|_||__:||||____||||||||________:||__||||:|_:|____||:_||_________||||:||\n______________GUAGUUG__CUGU__AACU_UA____CAGGCG_CGU____GG______________UGA__GAGU_GA__GUUUC____CUUACCGA________UAG__CCGUUU_UU____AUG_GU_________GGGGUAA\n3'-AUU...GAGAA_______UC__________G__A_________U___UCAA__UAUAUUACAACAG____AA____A__A______AUUA________AGCAACGU___AA______G__AGC____G__AGAGAAUAA_______AGAAA...CAA-5'\n_____________|_______________________________________________________________________________________________________________________________________|\n_____________163_____________________________________________________________________________________________________________________________________35\n |
226 -- 232 |
36 -- 42 |
-68.3253 kcal/mol |
25.8271 kcal/mol |
31.2699 kcal/mol |
116 233@LB@ | |@LB@5'-UGA...AUAUA CA GC G CGGC CUGA GAUUUAUUAUACAA GG GA GAG GU A A GAAU G AAGAA...CGG-3'@LB@ CGUCAGC GACA UUGA AU GUCCGC GCA CU ACU CUUA CU UAAAG GAAUGGCU GUC GGCAGA GA UAU CA CCCCGUU@LB@ |:|||:| |||| |||| || |||||| ||| |: ||| ||:| || :|||| |||||||| :|| ||||:| :| ||: || ||||:||@LB@ GUAGUUG CUGU AACU UA CAGGCG CGU GG UGA GAGU GA GUUUC CUUACCGA UAG CCGUUU UU AUG GU GGGGUAA@LB@3'-AUU...GAGAA UC G A U UCAA UAUAUUACAACAG AA A A AUUA AGCAACGU AA G AGC G AGAGAAUAA AGAAA...CAA-5'@LB@ | |@LB@ 163 35@LB@@LB@@LB@Energy -15.24 kcal/mol@LB@Hybridization Energy -68.3253 kcal/mol@LB@Unfolding Energy - mRNA 25.8271 kcal/mol@LB@Unfolding Energy - sRNA 31.2699 kcal/mol@LB@@LB@Position - mRNA 117 -- 232@LB@Position - sRNA 36 -- 162@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 100 |
0.004029 |
0.1561 |
bsu32980 |
yusZ |
-14.16 |
0.049671 |
3 -- 55 |
39 -- 90 |
oxidoreductase |
|
_____________2______________________________________56\n_____________|______________________________________|\n5'-CCG...GUCAU_____GUU_______GU____AUAG_____________AAUAG...CAA-3'\n______________UUCGU___CAGGAUA___ACA____CUGUAGCA_GUUU\n______________|||:|___|||||:|___|||____|:|:||||_|||:\n______________AAGUA___GUCCUGU___UGU____GGCGUCGU_CAAG\n3'-AUU...AUGAG_____GUU_______AAC___AACA________U____GUAUA...CAA-5'\n_____________|______________________________________|\n_____________91_____________________________________38\n |
175 -- 181 |
132 -- 138 |
-30.394 kcal/mol |
8.27302 kcal/mol |
10.1352 kcal/mol |
2 56@LB@ | |@LB@5'-CCG...GUCAU GUU GU AUAG AAUAG...CAA-3'@LB@ UUCGU CAGGAUA ACA CUGUAGCA GUUU@LB@ |||:| |||||:| ||| |:|:|||| |||:@LB@ AAGUA GUCCUGU UGU GGCGUCGU CAAG@LB@3'-AUU...AUGAG GUU AAC AACA U GUAUA...CAA-5'@LB@ | |@LB@ 91 38@LB@@LB@@LB@Energy -14.16 kcal/mol@LB@Hybridization Energy -30.394 kcal/mol@LB@Unfolding Energy - mRNA 8.27302 kcal/mol@LB@Unfolding Energy - sRNA 10.1352 kcal/mol@LB@@LB@Position - mRNA 3 -- 55@LB@Position - sRNA 39 -- 90@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 132 -- 138@LB@ |
| 158 |
0.007267 |
0.1751 |
bsu33000 |
htrB |
-17.04 |
0.012352 |
105 -- 237 |
50 -- 180 |
serine protease Do-like HtrB |
|
_____________104_____________________________238\n_____________|_______________________________|\n5'-UUU...GUGCU_______UCUCC______U______A_____ACUUG...CUG-3'\n______________UUGCUUU_____UCAUUA_UGGGAC_AUUGA\n______________||:||:|_____||||:|_::||||_|||||\n______________AAUGAGA_____AGUAGU_GUCCUG_UAACU\n3'-AUU...AAAAA__________________U____________GUAAC...CAA-5'\n_____________|_______________________________|\n_____________181_____________________________49\n |
110 -- 116 |
164 -- 170 |
-21.2082 kcal/mol |
7.59468 kcal/mol |
7.14638 kcal/mol |
104 238@LB@ | |@LB@5'-UUU...GUGCU UCUCC U A ACUUG...CUG-3'@LB@ UUGCUUU UCAUUA UGGGAC AUUGA@LB@ ||:||:| ||||:| ::|||| |||||@LB@ AAUGAGA AGUAGU GUCCUG UAACU@LB@3'-AUU...AAAAA U GUAAC...CAA-5'@LB@ | |@LB@ 181 49@LB@@LB@@LB@Energy -17.04 kcal/mol@LB@Hybridization Energy -21.2082 kcal/mol@LB@Unfolding Energy - mRNA 7.59468 kcal/mol@LB@Unfolding Energy - sRNA 7.14638 kcal/mol@LB@@LB@Position - mRNA 105 -- 237@LB@Position - sRNA 50 -- 180@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 164 -- 170@LB@ |
| 117 |
0.005342 |
0.1741 |
bsu33050 |
gerAA |
-9.34 |
0.402150 |
52 -- 63 |
38 -- 49 |
spore germination protein A1 |
|
_____________51___________64\n_____________|____________|\n5'-UAA...UUUCU____________GCUAG...UGC-3'\n______________UCUUGUUUUCCA\n______________||||:||::|||\n______________AGAAUAAGGGGU\n3'-AUU...GGUAG____________AAAGA...CAA-5'\n_____________|____________|\n_____________50___________37\n |
57 -- 63 |
38 -- 44 |
-12.8318 kcal/mol |
1.31908 kcal/mol |
4.13482 kcal/mol |
51 64@LB@ | |@LB@5'-UAA...UUUCU GCUAG...UGC-3'@LB@ UCUUGUUUUCCA@LB@ ||||:||::|||@LB@ AGAAUAAGGGGU@LB@3'-AUU...GGUAG AAAGA...CAA-5'@LB@ | |@LB@ 50 37@LB@@LB@@LB@Energy -9.34 kcal/mol@LB@Hybridization Energy -12.8318 kcal/mol@LB@Unfolding Energy - mRNA 1.31908 kcal/mol@LB@Unfolding Energy - sRNA 4.13482 kcal/mol@LB@@LB@Position - mRNA 52 -- 63@LB@Position - sRNA 38 -- 49@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 119 |
0.005369 |
0.1741 |
bsu33250 |
yvrL |
-8.38 |
0.555183 |
10 -- 36 |
76 -- 105 |
membrane-bound negative regulator YvrL |
|
_____________9__________37\n_____________|__________|\n5'-AUG...CCUUC__________UUGCG...AUA-3'\n______________AAAACGCUUA\n______________||||||||||\n______________UUUUGCGAAU\n3'-AUU...UAAAC__________UAAAG...CAA-5'\n_____________|__________|\n_____________106________75\n |
224 -- 230 |
13 -- 19 |
-10.8458 kcal/mol |
2.09283 kcal/mol |
3.31287 kcal/mol |
9 37@LB@ | |@LB@5'-AUG...CCUUC UUGCG...AUA-3'@LB@ AAAACGCUUA@LB@ ||||||||||@LB@ UUUUGCGAAU@LB@3'-AUU...UAAAC UAAAG...CAA-5'@LB@ | |@LB@ 106 75@LB@@LB@@LB@Energy -8.38 kcal/mol@LB@Hybridization Energy -10.8458 kcal/mol@LB@Unfolding Energy - mRNA 2.09283 kcal/mol@LB@Unfolding Energy - sRNA 3.31287 kcal/mol@LB@@LB@Position - mRNA 10 -- 36@LB@Position - sRNA 76 -- 105@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 19 |
0.0005622 |
0.1011 |
bsu33310 |
fhuB |
-20.25 |
0.002499 |
30 -- 116 |
99 -- 184 |
iron(3+)-hydroxamate import system permease protein FhuB |
bsu01620 |
_____________29__________________________________________________________________117\n_____________|___________________________________________________________________|\n5'-AGG...UGCAA___A__________AAAAA____________UCC_______UU_____U___G______A_______ACCUU...AAG-3'\n______________UCA_UAACAGGGCA___________CGCGGC___GAGUUUC__GUAUA_AUG__GUCAU__UCUCAU\n______________|||_:||||||:||___________|||:||___:||||:|__:||||_|||__||||:__||||||\n______________AGU_GUUGUCCUGU___________GCGUCG___UUCAAGG__UAUAU_UAC__CAGUG__AGAGUA\n3'-AUU...UGAGA___A__________AACUGUAACAG___________________________AA_____AA______GAAGU...CAA-5'\n_____________|___________________________________________________________________|\n_____________185_________________________________________________________________98\n |
80 -- 86 |
126 -- 132 |
-44.6592 kcal/mol |
13.3222 kcal/mol |
14.475 kcal/mol |
29 117@LB@ | |@LB@5'-AGG...UGCAA A AAAAA UCC UU U G A ACCUU...AAG-3'@LB@ UCA UAACAGGGCA CGCGGC GAGUUUC GUAUA AUG GUCAU UCUCAU@LB@ ||| :||||||:|| |||:|| :||||:| :|||| ||| ||||: ||||||@LB@ AGU GUUGUCCUGU GCGUCG UUCAAGG UAUAU UAC CAGUG AGAGUA@LB@3'-AUU...UGAGA A AACUGUAACAG AA AA GAAGU...CAA-5'@LB@ | |@LB@ 185 98@LB@@LB@@LB@Energy -20.25 kcal/mol@LB@Hybridization Energy -44.6592 kcal/mol@LB@Unfolding Energy - mRNA 13.3222 kcal/mol@LB@Unfolding Energy - sRNA 14.475 kcal/mol@LB@@LB@Position - mRNA 30 -- 116@LB@Position - sRNA 99 -- 184@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 126 -- 132@LB@ |
