| 141 |
0.006569 |
0.1741 |
bsu19520 |
yojA |
-12.26 |
0.119762 |
158 -- 180 |
120 -- 146 |
permease YojA |
bsu40070 |
_____________157_________________________________________________________181\n_____________|___________________________________________________________|\n5'-GGU...GCUUU__G________________GAAAAGCGCCAAA_________________AA________AACAC...UUU-3'\n______________CU_UUCAUC__UAGG_CAU_____________ACAUUGUC____GCGAG__UCAUAUAA\n______________||_||||||__:|||_|||_____________||||||||____||:||__:|||||||\n______________GA_AAGUAG__GUCC_GUA_____________UGUAACAG____CGUUC__GGUAUAUU\n3'-AUU...AAAAU__G______UU____U___AC___________________GCGU_____AA________ACAAC...CAA-5'\n_____________|___________________________________________________________|\n_____________147_________________________________________________________119\n |
174 -- 180 |
120 -- 126 |
-32.1826 kcal/mol |
10.1468 kcal/mol |
10.5645 kcal/mol |
157 181@LB@ | |@LB@5'-GGU...GCUUU G GAAAAGCGCCAAA AA AACAC...UUU-3'@LB@ CU UUCAUC UAGG CAU ACAUUGUC GCGAG UCAUAUAA@LB@ || |||||| :||| ||| |||||||| ||:|| :|||||||@LB@ GA AAGUAG GUCC GUA UGUAACAG CGUUC GGUAUAUU@LB@3'-AUU...AAAAU G UU U AC GCGU AA ACAAC...CAA-5'@LB@ | |@LB@ 147 119@LB@@LB@@LB@Energy -12.26 kcal/mol@LB@Hybridization Energy -32.1826 kcal/mol@LB@Unfolding Energy - mRNA 10.1468 kcal/mol@LB@Unfolding Energy - sRNA 10.5645 kcal/mol@LB@@LB@Position - mRNA 158 -- 180@LB@Position - sRNA 120 -- 146@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 181 |
0.008392 |
0.1751 |
bsu19600 |
yodH |
-10.70 |
0.236480 |
130 -- 154 |
156 -- 183 |
methyltransferase |
|
_____________129______________155\n_____________|________________|\n5'-AAC...CUUCU____________A___CUAUG...CCU-3'\n______________UUUGCUUUUCAU_AAC\n______________|||:||:|||||_|||\n______________AAAUGAGAAGUA_UUG\n3'-AUU...AAAAA____________G___UCCUG...CAA-5'\n_____________|________________|\n_____________184______________155\n |
144 -- 150 |
160 -- 166 |
-13.2566 kcal/mol |
3.14685 kcal/mol |
1.41519 kcal/mol |
129 155@LB@ | |@LB@5'-AAC...CUUCU A CUAUG...CCU-3'@LB@ UUUGCUUUUCAU AAC@LB@ |||:||:||||| |||@LB@ AAAUGAGAAGUA UUG@LB@3'-AUU...AAAAA G UCCUG...CAA-5'@LB@ | |@LB@ 184 155@LB@@LB@@LB@Energy -10.70 kcal/mol@LB@Hybridization Energy -13.2566 kcal/mol@LB@Unfolding Energy - mRNA 3.14685 kcal/mol@LB@Unfolding Energy - sRNA 1.41519 kcal/mol@LB@@LB@Position - mRNA 130 -- 154@LB@Position - sRNA 156 -- 183@LB@Position Seed - mRNA 144 -- 150@LB@Position Seed - sRNA 160 -- 166@LB@ |
| 38 |
0.001252 |
0.1206 |
bsu19610 |
yodI |
-17.13 |
0.011794 |
8 -- 27 |
49 -- 68 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________7____________________28\n_____________|____________________|\n____5'-AUUUAUC___________AU_______AUUAC...UAA-3'\n______________GCAAACGAUUG__CCCAUCU\n______________||||||:||:|__|||||||\n______________CGUUUGUUAGC__GGGUAGA\n3'-AUU...AGAAC___________AU_______GAAUA...CAA-5'\n_____________|____________________|\n_____________69___________________48\n |
21 -- 27 |
49 -- 55 |
-26.3851 kcal/mol |
1.36402 kcal/mol |
8.38306 kcal/mol |
7 28@LB@ | |@LB@ 5'-AUUUAUC AU AUUAC...UAA-3'@LB@ GCAAACGAUUG CCCAUCU@LB@ ||||||:||:| |||||||@LB@ CGUUUGUUAGC GGGUAGA@LB@3'-AUU...AGAAC AU GAAUA...CAA-5'@LB@ | |@LB@ 69 48@LB@@LB@@LB@Energy -17.13 kcal/mol@LB@Hybridization Energy -26.3851 kcal/mol@LB@Unfolding Energy - mRNA 1.36402 kcal/mol@LB@Unfolding Energy - sRNA 8.38306 kcal/mol@LB@@LB@Position - mRNA 8 -- 27@LB@Position - sRNA 49 -- 68@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 130 |
0.005995 |
0.1741 |
bsu19660 |
yozD |
-10.24 |
0.285413 |
18 -- 51 |
105 -- 135 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________17_________________________________52\n_____________|__________________________________|\n5'-CAC...CUUUG____UUUU_____U______AUCAAAU_______CGUCC...UGU-3'\n______________AGCG____UUCCA_UAUGAU_______CUUUCUC\n______________|||:____|||||_|||:||_______|||||||\n______________UCGU____AAGGU_AUAUUA_______GAAAGAG\n3'-AUU...AGGCG____UC______________CAACAGU_______UAGAA...CAA-5'\n_____________|__________________________________|\n_____________136________________________________104\n |
45 -- 51 |
105 -- 111 |
-19.2876 kcal/mol |
2.67508 kcal/mol |
6.61557 kcal/mol |
17 52@LB@ | |@LB@5'-CAC...CUUUG UUUU U AUCAAAU CGUCC...UGU-3'@LB@ AGCG UUCCA UAUGAU CUUUCUC@LB@ |||: ||||| |||:|| |||||||@LB@ UCGU AAGGU AUAUUA GAAAGAG@LB@3'-AUU...AGGCG UC CAACAGU UAGAA...CAA-5'@LB@ | |@LB@ 136 104@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -19.2876 kcal/mol@LB@Unfolding Energy - mRNA 2.67508 kcal/mol@LB@Unfolding Energy - sRNA 6.61557 kcal/mol@LB@@LB@Position - mRNA 18 -- 51@LB@Position - sRNA 105 -- 135@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 61 |
0.001915 |
0.1214 |
bsu19720 |
yodR |
-8.11 |
0.600668 |
13 -- 29 |
71 -- 89 |
coenzyme A transferase subunit beta |
|
_____________12___________30\n_____________|____________|\n5'-GCU...GAAGA____A_______ACCGG...CCU-3'\n______________AAUG_UUGUCAC\n______________||||_|||||||\n______________UUAC_AACAGUG\n3'-AUU...GUAUA____________AAAGA...CAA-5'\n_____________|____________|\n_____________90___________70\n |
143 -- 149 |
111 -- 117 |
-12.213 kcal/mol |
1.50216 kcal/mol |
3.63424 kcal/mol |
12 30@LB@ | |@LB@5'-GCU...GAAGA A ACCGG...CCU-3'@LB@ AAUG UUGUCAC@LB@ |||| |||||||@LB@ UUAC AACAGUG@LB@3'-AUU...GUAUA AAAGA...CAA-5'@LB@ | |@LB@ 90 70@LB@@LB@@LB@Energy -8.11 kcal/mol@LB@Hybridization Energy -12.213 kcal/mol@LB@Unfolding Energy - mRNA 1.50216 kcal/mol@LB@Unfolding Energy - sRNA 3.63424 kcal/mol@LB@@LB@Position - mRNA 13 -- 29@LB@Position - sRNA 71 -- 89@LB@Position Seed - mRNA 143 -- 149@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 122 |
0.005613 |
0.1741 |
bsu19770 |
cgeC |
-14.56 |
0.041162 |
73 -- 119 |
6 -- 59 |
spore maturation protein CgeC |
|
_____________72_______________________________________________________120\n_____________|________________________________________________________|\n5'-CAC...AACGC_______G____AUUU___A_______AAG__________________________AAUGG...AAG-3'\n______________CGUACCU_AUCU____UUC_UCUAUUU___________GAAAAUGCU_GAUUUUUU\n______________||||||:_||||____|||_:|:||||___________|||||:|||_:||||:||\n______________GCAUGGG_UAGA____AAG_GGGUAAA___________CUUUUGCGA_UUAAAGAA\n3'-AUU...UGUUA____________GAAU___________GAAACUUUAAA_________A________AACAA-5'\n_____________|________________________________________________________|\n_____________60_______________________________________________________5\n |
105 -- 111 |
15 -- 21 |
-32.5895 kcal/mol |
8.55003 kcal/mol |
11.8347 kcal/mol |
72 120@LB@ | |@LB@5'-CAC...AACGC G AUUU A AAG AAUGG...AAG-3'@LB@ CGUACCU AUCU UUC UCUAUUU GAAAAUGCU GAUUUUUU@LB@ ||||||: |||| ||| :|:|||| |||||:||| :||||:||@LB@ GCAUGGG UAGA AAG GGGUAAA CUUUUGCGA UUAAAGAA@LB@3'-AUU...UGUUA GAAU GAAACUUUAAA A AACAA-5'@LB@ | |@LB@ 60 5@LB@@LB@@LB@Energy -14.56 kcal/mol@LB@Hybridization Energy -32.5895 kcal/mol@LB@Unfolding Energy - mRNA 8.55003 kcal/mol@LB@Unfolding Energy - sRNA 11.8347 kcal/mol@LB@@LB@Position - mRNA 73 -- 119@LB@Position - sRNA 6 -- 59@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 33 |
0.0009705 |
0.114 |
bsu20780 |
yopS |
-12.53 |
0.106187 |
15 -- 36 |
34 -- 54 |
XRE family transcriptional regulator |
|
_____________14_______________37\n_____________|________________|\n5'-GUU...ACCUG________C_______UAUGU...AAC-3'\n______________CCAUC_CU_AUUCUCC\n______________|||||_||_||||:||\n______________GGUAG_GA_UAAGGGG\n3'-AUU...GCAUG_____A__A_______UAAAG...CAA-5'\n_____________|________________|\n_____________55_______________33\n |
23 -- 29 |
39 -- 45 |
-17.4753 kcal/mol |
0.463886 kcal/mol |
7.9005 kcal/mol |
14 37@LB@ | |@LB@5'-GUU...ACCUG C UAUGU...AAC-3'@LB@ CCAUC CU AUUCUCC@LB@ ||||| || ||||:||@LB@ GGUAG GA UAAGGGG@LB@3'-AUU...GCAUG A A UAAAG...CAA-5'@LB@ | |@LB@ 55 33@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -17.4753 kcal/mol@LB@Unfolding Energy - mRNA 0.463886 kcal/mol@LB@Unfolding Energy - sRNA 7.9005 kcal/mol@LB@@LB@Position - mRNA 15 -- 36@LB@Position - sRNA 34 -- 54@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 54 |
0.001793 |
0.1212 |
bsu21030 |
yonP |
-11.11 |
0.198660 |
20 -- 36 |
161 -- 178 |
Evidence 5: No homology to any previously reported sequences |
|
_____________19_______37\n_____________|________|\n5'-AAU...UUUAA________AUAUU...ACA-3'\n______________ACUCUUUA\n______________||||||:|\n______________UGAGAAGU\n3'-AUU...AAAAA________AGUUG...CAA-5'\n_____________|________|\n_____________179______160\n |
30 -- 36 |
161 -- 167 |
-8.6372 kcal/mol |
0.252156 kcal/mol |
0.576912 kcal/mol |
19 37@LB@ | |@LB@5'-AAU...UUUAA AUAUU...ACA-3'@LB@ ACUCUUUA@LB@ ||||||:|@LB@ UGAGAAGU@LB@3'-AUU...AAAAA AGUUG...CAA-5'@LB@ | |@LB@ 179 160@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -8.6372 kcal/mol@LB@Unfolding Energy - mRNA 0.252156 kcal/mol@LB@Unfolding Energy - sRNA 0.576912 kcal/mol@LB@@LB@Position - mRNA 20 -- 36@LB@Position - sRNA 161 -- 178@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 161 -- 167@LB@ |
| 58 |
0.001848 |
0.1212 |
bsu21840 |
ypjP |
-10.31 |
0.277541 |
15 -- 73 |
14 -- 70 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________14___________________________________________________________74\n_____________|____________________________________________________________|\n5'-UAA...AACUG__________AG___CAA____GCGGAAAG____C____A_______A___U________UUACA...CCG-3'\n______________UGGCAAACAG__CGU___UCAU________CCUC_UUUC__UGAAAU_UUG_AAGCGUUU\n______________|||||||||:__|||___:|||________||:|_||||__||||||_|||_||:||:||\n______________ACCGUUUGUU__GCA___GGUA________GGGG_AAAG__ACUUUA_AAC_UUUGCGAA\n3'-AUU...UUAGA__________A____UG_____GAGAAUAA____U____AA__________U________UUAAA...CAA-5'\n_____________|____________________________________________________________|\n_____________71___________________________________________________________13\n |
67 -- 73 |
14 -- 20 |
-38.3558 kcal/mol |
13.3253 kcal/mol |
15.5628 kcal/mol |
14 74@LB@ | |@LB@5'-UAA...AACUG AG CAA GCGGAAAG C A A U UUACA...CCG-3'@LB@ UGGCAAACAG CGU UCAU CCUC UUUC UGAAAU UUG AAGCGUUU@LB@ |||||||||: ||| :||| ||:| |||| |||||| ||| ||:||:||@LB@ ACCGUUUGUU GCA GGUA GGGG AAAG ACUUUA AAC UUUGCGAA@LB@3'-AUU...UUAGA A UG GAGAAUAA U AA U UUAAA...CAA-5'@LB@ | |@LB@ 71 13@LB@@LB@@LB@Energy -10.31 kcal/mol@LB@Hybridization Energy -38.3558 kcal/mol@LB@Unfolding Energy - mRNA 13.3253 kcal/mol@LB@Unfolding Energy - sRNA 15.5628 kcal/mol@LB@@LB@Position - mRNA 15 -- 73@LB@Position - sRNA 14 -- 70@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 14 -- 20@LB@ |
| 118 |
0.005364 |
0.1741 |
bsu21980 |
ypdP |
-14.81 |
0.036422 |
190 -- 236 |
72 -- 112 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________189_______________________________________________237\n_____________|_________________________________________________|\n5'-AAC...AGGAA_____________GUUUAAUAAUUC__UU___________U________AUUCA...GGA-3'\n______________GCUUUUUCAUCUU____________GU__UGGAU__CUUU_UUGCGAUC\n______________:||||:|||||||____________||__||:||__|||:_||||:|||\n______________UGAAAGAGUAGAA____________CA__ACUUA__GAAG_AACGUUAG\n3'-AUU...AACAG_____________GUUU__________UU_____CC____C________AACCG...CAA-5'\n_____________|_________________________________________________|\n_____________113_______________________________________________71\n |
230 -- 236 |
72 -- 78 |
-29.9578 kcal/mol |
5.88063 kcal/mol |
12.3236 kcal/mol |
189 237@LB@ | |@LB@5'-AAC...AGGAA GUUUAAUAAUUC UU U AUUCA...GGA-3'@LB@ GCUUUUUCAUCUU GU UGGAU CUUU UUGCGAUC@LB@ :||||:||||||| || ||:|| |||: ||||:|||@LB@ UGAAAGAGUAGAA CA ACUUA GAAG AACGUUAG@LB@3'-AUU...AACAG GUUU UU CC C AACCG...CAA-5'@LB@ | |@LB@ 113 71@LB@@LB@@LB@Energy -14.81 kcal/mol@LB@Hybridization Energy -29.9578 kcal/mol@LB@Unfolding Energy - mRNA 5.88063 kcal/mol@LB@Unfolding Energy - sRNA 12.3236 kcal/mol@LB@@LB@Position - mRNA 190 -- 236@LB@Position - sRNA 72 -- 112@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 72 -- 78@LB@ |
