| 96 |
0.003858 |
0.1541 |
bsu03700 |
gerKA |
-12.88 |
0.090279 |
45 -- 139 |
38 -- 125 |
spore germination protein KA |
|
_____________44_________________________________________140\n_____________|__________________________________________|\n5'-UUG...CCUCC_____UAG___CU___U________GA____A____G_____AACCC...CGG-3'\n______________GCUUU___UGU__AUC_UUGGCGGA__AGUU_UACC_CAUCU\n______________||||:___||:__|||_|||||::|__|:|:_||||_|||||\n______________CGAAG___ACG__UAG_AACCGUUU__UUAG_AUGG_GUAGA\n3'-AUU...CUUAC_____CA____U_____________G_____C__________GAAUA...CAA-5'\n_____________|__________________________________________|\n_____________126________________________________________37\n |
108 -- 114 |
65 -- 71 |
-29.7431 kcal/mol |
10.7852 kcal/mol |
9.08698 kcal/mol |
44 140@LB@ | |@LB@5'-UUG...CCUCC UAG CU U GA A G AACCC...CGG-3'@LB@ GCUUU UGU AUC UUGGCGGA AGUU UACC CAUCU@LB@ ||||: ||: ||| |||||::| |:|: |||| |||||@LB@ CGAAG ACG UAG AACCGUUU UUAG AUGG GUAGA@LB@3'-AUU...CUUAC CA U G C GAAUA...CAA-5'@LB@ | |@LB@ 126 37@LB@@LB@@LB@Energy -12.88 kcal/mol@LB@Hybridization Energy -29.7431 kcal/mol@LB@Unfolding Energy - mRNA 10.7852 kcal/mol@LB@Unfolding Energy - sRNA 9.08698 kcal/mol@LB@@LB@Position - mRNA 45 -- 139@LB@Position - sRNA 38 -- 125@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 65 -- 71@LB@ |
| 105 |
0.004352 |
0.1606 |
bsu05340 |
ydfA |
-10.81 |
0.225539 |
212 -- 245 |
38 -- 75 |
arsenic pump protein |
|
_____________211____________________________246\n_____________|______________________________|\n5'-AGC...UCAAU______________________________UAUUA...GAG-3'\n______________GAUUUUGGCA_______GUAUUCAUCUUUU\n______________:||:||||||_______|||::|||||:||\n______________UUAGAACCGU_______CAUGGGUAGAGAA\n3'-AUU...CAACG__________UUGUUAG_____________UAAGG...CAA-5'\n_____________|______________________________|\n_____________76_____________________________37\n |
227 -- 233 |
47 -- 53 |
-24.4224 kcal/mol |
4.10196 kcal/mol |
8.49986 kcal/mol |
211 246@LB@ | |@LB@5'-AGC...UCAAU UAUUA...GAG-3'@LB@ GAUUUUGGCA GUAUUCAUCUUUU@LB@ :||:|||||| |||::|||||:||@LB@ UUAGAACCGU CAUGGGUAGAGAA@LB@3'-AUU...CAACG UUGUUAG UAAGG...CAA-5'@LB@ | |@LB@ 76 37@LB@@LB@@LB@Energy -10.81 kcal/mol@LB@Hybridization Energy -24.4224 kcal/mol@LB@Unfolding Energy - mRNA 4.10196 kcal/mol@LB@Unfolding Energy - sRNA 8.49986 kcal/mol@LB@@LB@Position - mRNA 212 -- 245@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 113 |
0.005141 |
0.1741 |
bsu36080 |
ywrF |
-10.09 |
0.302867 |
265 -- 274 |
38 -- 47 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________264________275\n_____________|__________|\n5'-UGG...UACCG__________CCCCA...UUU-3'\n______________UUAUUCCCCG\n______________|||||||||:\n______________AAUAAGGGGU\n3'-AUU...UAGAG__________AAAGA...CAA-5'\n_____________|__________|\n_____________48_________37\n |
268 -- 274 |
38 -- 44 |
-13.6226 kcal/mol |
1.45475 kcal/mol |
3.86656 kcal/mol |
264 275@LB@ | |@LB@5'-UGG...UACCG CCCCA...UUU-3'@LB@ UUAUUCCCCG@LB@ |||||||||:@LB@ AAUAAGGGGU@LB@3'-AUU...UAGAG AAAGA...CAA-5'@LB@ | |@LB@ 48 37@LB@@LB@@LB@Energy -10.09 kcal/mol@LB@Hybridization Energy -13.6226 kcal/mol@LB@Unfolding Energy - mRNA 1.45475 kcal/mol@LB@Unfolding Energy - sRNA 3.86656 kcal/mol@LB@@LB@Position - mRNA 265 -- 274@LB@Position - sRNA 38 -- 47@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 114 |
0.005219 |
0.1741 |
bsu18630 |
exlX |
-14.36 |
0.045196 |
231 -- 263 |
38 -- 88 |
expansin-YoaJ |
|
_____________230________________________________________________________264\n_____________|__________________________________________________________|\n5'-GGG...AGAUC_______CA_______GUA________UU________U____________________CAAGC...GUU-3'\n______________AUGAGUG__UUUGUUG___UGGUUUUG___GACGAUC___UCUGUUUU____UCCCCG\n______________||||:||__||:||||___:::|:|||___:||:|||___:|::|:|:____|||||:\n______________UACUUAC__AAGCAAC___GUUAGAAC___UUGUUAG___GGGUAGAG____AGGGGU\n3'-AUU...UUCAU_______CG__________________CGU_______CAU________AAUA______AAAGA...CAA-5'\n_____________|__________________________________________________________|\n_____________89_________________________________________________________37\n |
242 -- 248 |
59 -- 65 |
-36.6264 kcal/mol |
9.40493 kcal/mol |
14.3562 kcal/mol |
230 264@LB@ | |@LB@5'-GGG...AGAUC CA GUA UU U CAAGC...GUU-3'@LB@ AUGAGUG UUUGUUG UGGUUUUG GACGAUC UCUGUUUU UCCCCG@LB@ ||||:|| ||:|||| :::|:||| :||:||| :|::|:|: |||||:@LB@ UACUUAC AAGCAAC GUUAGAAC UUGUUAG GGGUAGAG AGGGGU@LB@3'-AUU...UUCAU CG CGU CAU AAUA AAAGA...CAA-5'@LB@ | |@LB@ 89 37@LB@@LB@@LB@Energy -14.36 kcal/mol@LB@Hybridization Energy -36.6264 kcal/mol@LB@Unfolding Energy - mRNA 9.40493 kcal/mol@LB@Unfolding Energy - sRNA 14.3562 kcal/mol@LB@@LB@Position - mRNA 231 -- 263@LB@Position - sRNA 38 -- 88@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 117 |
0.005342 |
0.1741 |
bsu33050 |
gerAA |
-9.34 |
0.402150 |
52 -- 63 |
38 -- 49 |
spore germination protein A1 |
|
_____________51___________64\n_____________|____________|\n5'-UAA...UUUCU____________GCUAG...UGC-3'\n______________UCUUGUUUUCCA\n______________||||:||::|||\n______________AGAAUAAGGGGU\n3'-AUU...GGUAG____________AAAGA...CAA-5'\n_____________|____________|\n_____________50___________37\n |
57 -- 63 |
38 -- 44 |
-12.8318 kcal/mol |
1.31908 kcal/mol |
4.13482 kcal/mol |
51 64@LB@ | |@LB@5'-UAA...UUUCU GCUAG...UGC-3'@LB@ UCUUGUUUUCCA@LB@ ||||:||::|||@LB@ AGAAUAAGGGGU@LB@3'-AUU...GGUAG AAAGA...CAA-5'@LB@ | |@LB@ 50 37@LB@@LB@@LB@Energy -9.34 kcal/mol@LB@Hybridization Energy -12.8318 kcal/mol@LB@Unfolding Energy - mRNA 1.31908 kcal/mol@LB@Unfolding Energy - sRNA 4.13482 kcal/mol@LB@@LB@Position - mRNA 52 -- 63@LB@Position - sRNA 38 -- 49@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 156 |
0.007194 |
0.1751 |
bsu12900 |
htrA |
-15.16 |
0.030869 |
26 -- 118 |
38 -- 130 |
serine protease Do-like HtrA |
|
_____________25_______________________________________________________________________________________________________119\n_____________|________________________________________________________________________________________________________|\n5'-CUG...AAUCU_______CGACUCA__________A___A_____U________GU__AC____UUCC___C____UC_________U_______AA____UUCAUUUUUA____ACAGU...AUG-3'\n______________GUUCCAU________GUC_CUUUC_UAU___CAA____AUGAA__GU__CGUU____GCA_UUUU_____ACAAUU___CCCAU__UCUU__________UCCC\n______________|||||||________|||_|||||_:||___|||____|||||__|:__||||____|||_|:||_____|||||:___|||||__||||__________||||\n______________CAAGGUA________CAG_GAAAG_GUA___GUU____UACUU__CG__GCAA____CGU_AGAA_____UGUUAG___GGGUA__AGAA__________AGGG\n3'-AUU...UCGUU_______UAUUACAA___U_____A___GAA___UCAU_____AC__AA___________U____CCGUU______CAU_____G_____UA____________GUAAA...CAA-5'\n_____________|________________________________________________________________________________________________________|\n_____________131______________________________________________________________________________________________________37\n |
26 -- 32 |
124 -- 130 |
-40.6941 kcal/mol |
8.96571 kcal/mol |
22.8172 kcal/mol |
25 119@LB@ | |@LB@5'-CUG...AAUCU CGACUCA A A U GU AC UUCC C UC U AA UUCAUUUUUA ACAGU...AUG-3'@LB@ GUUCCAU GUC CUUUC UAU CAA AUGAA GU CGUU GCA UUUU ACAAUU CCCAU UCUU UCCC@LB@ ||||||| ||| ||||| :|| ||| ||||| |: |||| ||| |:|| |||||: ||||| |||| ||||@LB@ CAAGGUA CAG GAAAG GUA GUU UACUU CG GCAA CGU AGAA UGUUAG GGGUA AGAA AGGG@LB@3'-AUU...UCGUU UAUUACAA U A GAA UCAU AC AA U CCGUU CAU G UA GUAAA...CAA-5'@LB@ | |@LB@ 131 37@LB@@LB@@LB@Energy -15.16 kcal/mol@LB@Hybridization Energy -40.6941 kcal/mol@LB@Unfolding Energy - mRNA 8.96571 kcal/mol@LB@Unfolding Energy - sRNA 22.8172 kcal/mol@LB@@LB@Position - mRNA 26 -- 118@LB@Position - sRNA 38 -- 130@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 124 -- 130@LB@ |
| 164 |
0.007653 |
0.1751 |
bsu02110 |
ybyB |
-7.82 |
0.651200 |
271 -- 286 |
38 -- 56 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________270_____287\n_____________|_______|\n5'-UUG...AAAAG_______AAAUC...AAU-3'\n______________ACCCGUC\n______________||||:||\n______________UGGGUAG\n3'-AUU...UAGCA_______AGAAU...CAA-5'\n_____________|_______|\n_____________57______37\n |
271 -- 277 |
50 -- 56 |
-11.5721 kcal/mol |
0.816393 kcal/mol |
3.06627 kcal/mol |
270 287@LB@ | |@LB@5'-UUG...AAAAG AAAUC...AAU-3'@LB@ ACCCGUC@LB@ ||||:||@LB@ UGGGUAG@LB@3'-AUU...UAGCA AGAAU...CAA-5'@LB@ | |@LB@ 57 37@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -11.5721 kcal/mol@LB@Unfolding Energy - mRNA 0.816393 kcal/mol@LB@Unfolding Energy - sRNA 3.06627 kcal/mol@LB@@LB@Position - mRNA 271 -- 286@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 196 |
0.008988 |
0.1751 |
bsu24970 |
pstA |
-9.97 |
0.317060 |
128 -- 176 |
38 -- 86 |
ABC transporter permease |
|
_____________127___________________________________________________177\n_____________|_____________________________________________________|\n5'-AUU...AAUGG___________U__________UGU__U__U________CUG_______G___CUUGU...UUA-3'\n______________GGCUU_G_UGC__UC_UGGUGA___CA_UC_UGUUCAUC___CUUAUUC_CUA\n______________|||||_|_|||__||_|||::|___||_||_|:::||||___|||||||_|:|\n______________CCGAA_C_ACG__AG_ACCGUU___GU_AG_AUGGGUAG___GAAUAAG_GGU\n3'-AUU...ACUUA_____G_A___UU__A______U____U__C________A_________G___AAAGA...CAA-5'\n_____________|_____________________________________________________|\n_____________87____________________________________________________37\n |
166 -- 172 |
42 -- 48 |
-33.9261 kcal/mol |
12.9253 kcal/mol |
13.9524 kcal/mol |
127 177@LB@ | |@LB@5'-AUU...AAUGG U UGU U U CUG G CUUGU...UUA-3'@LB@ GGCUU G UGC UC UGGUGA CA UC UGUUCAUC CUUAUUC CUA@LB@ ||||| | ||| || |||::| || || |:::|||| ||||||| |:|@LB@ CCGAA C ACG AG ACCGUU GU AG AUGGGUAG GAAUAAG GGU@LB@3'-AUU...ACUUA G A UU A U U C A G AAAGA...CAA-5'@LB@ | |@LB@ 87 37@LB@@LB@@LB@Energy -9.97 kcal/mol@LB@Hybridization Energy -33.9261 kcal/mol@LB@Unfolding Energy - mRNA 12.9253 kcal/mol@LB@Unfolding Energy - sRNA 13.9524 kcal/mol@LB@@LB@Position - mRNA 128 -- 176@LB@Position - sRNA 38 -- 86@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 32 |
0.0008744 |
0.1059 |
bsu09430 |
citR |
-15.39 |
0.027619 |
23 -- 103 |
39 -- 111 |
LysR family transcriptional regulator |
|
_____________22______________________________________________________________________________________104\n_____________|_______________________________________________________________________________________|\n5'-UGU...ACGCC________C_A____UAU__C______________UACACA_______CCCUU________CGUAAU__________G_________CUAUC...CCG-3'\n______________CUUUUUCA_C_UCAA___GG_UGAUGGAU_GUUUC______UGUAAUU________UAAUC______GUA_CCAUUU__UAUUCCCC\n______________||||:|||_|_||||___:|_|:|||:||_|:|||______||:|||:________:||||______|||_||||:|__||||||||\n______________GAAAGAGU_G_AGUU___UC_AUUACUUA_CGAAG______ACGUUAG________GUUAG______CAU_GGUAGA__AUAAGGGG\n3'-AUU...ACAGU________A_A__________________C_____CA___________AACCGUUU______________G______GA________UAAAG...CAA-5'\n_____________|_______________________________________________________________________________________|\n_____________112_____________________________________________________________________________________38\n |
97 -- 103 |
39 -- 45 |
-43.1846 kcal/mol |
11.0009 kcal/mol |
20.821 kcal/mol |
22 104@LB@ | |@LB@5'-UGU...ACGCC C A UAU C UACACA CCCUU CGUAAU G CUAUC...CCG-3'@LB@ CUUUUUCA C UCAA GG UGAUGGAU GUUUC UGUAAUU UAAUC GUA CCAUUU UAUUCCCC@LB@ ||||:||| | |||| :| |:|||:|| |:||| ||:|||: :|||| ||| ||||:| ||||||||@LB@ GAAAGAGU G AGUU UC AUUACUUA CGAAG ACGUUAG GUUAG CAU GGUAGA AUAAGGGG@LB@3'-AUU...ACAGU A A C CA AACCGUUU G GA UAAAG...CAA-5'@LB@ | |@LB@ 112 38@LB@@LB@@LB@Energy -15.39 kcal/mol@LB@Hybridization Energy -43.1846 kcal/mol@LB@Unfolding Energy - mRNA 11.0009 kcal/mol@LB@Unfolding Energy - sRNA 20.821 kcal/mol@LB@@LB@Position - mRNA 23 -- 103@LB@Position - sRNA 39 -- 111@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 48 |
0.001494 |
0.1206 |
bsu33660 |
rghRA |
-13.24 |
0.076349 |
28 -- 146 |
39 -- 166 |
HTH-type transcriptional repressor RghR |
bsu33670 |
_____________27_________________________________________________147\n_____________|__________________________________________________|\n5'-UAU...AUUAC_____A____AAC______U___U___U_______A____A_C_______UUUAG...UCU-3'\n______________GGCUU_UGUU____UUUUG_CAA_ACA_UU___CC_GUCU_C_UAUUCCC\n______________|||||_:|||____|:|||_|||_|||_|:___||_:|||_|_|||||||\n______________CCGAA_GCAA____AGAAC_GUU_UGU_AG___GG_UAGA_G_AUAAGGG\n3'-AUU...ACUUA__________CGUU_____C_______U__CAU__G______A_______GUAAA...CAA-5'\n_____________|__________________________________________________|\n_____________167________________________________________________38\n |
139 -- 145 |
40 -- 46 |
-27.8785 kcal/mol |
6.21563 kcal/mol |
10.9223 kcal/mol |
27 147@LB@ | |@LB@5'-UAU...AUUAC A AAC U U U A A C UUUAG...UCU-3'@LB@ GGCUU UGUU UUUUG CAA ACA UU CC GUCU C UAUUCCC@LB@ ||||| :||| |:||| ||| ||| |: || :||| | |||||||@LB@ CCGAA GCAA AGAAC GUU UGU AG GG UAGA G AUAAGGG@LB@3'-AUU...ACUUA CGUU C U CAU G A GUAAA...CAA-5'@LB@ | |@LB@ 167 38@LB@@LB@@LB@Energy -13.24 kcal/mol@LB@Hybridization Energy -27.8785 kcal/mol@LB@Unfolding Energy - mRNA 6.21563 kcal/mol@LB@Unfolding Energy - sRNA 10.9223 kcal/mol@LB@@LB@Position - mRNA 28 -- 146@LB@Position - sRNA 39 -- 166@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 40 -- 46@LB@ |
