| 104 |
0.004338 |
0.1606 |
bsu08000 |
yfjQ |
-9.77 |
0.342120 |
136 -- 162 |
103 -- 137 |
metal ion transporter YfjQ |
bsu24740 |
_____________135_______________163\n_____________|_________________|\n5'-CAU...AUUAG____A____A_______CUUUU...AUU-3'\n______________GCAG_GAGU_CCGUAUA\n______________||||_:|||_||:||||\n______________CGUC_UUCA_GGUAUAU\n3'-AUU...ACAGG____G____A_______UACAA...CAA-5'\n_____________|_________________|\n_____________138_______________102\n |
146 -- 152 |
121 -- 127 |
-16.4629 kcal/mol |
4.99509 kcal/mol |
3.33887 kcal/mol |
135 163@LB@ | |@LB@5'-CAU...AUUAG A A CUUUU...AUU-3'@LB@ GCAG GAGU CCGUAUA@LB@ |||| :||| ||:||||@LB@ CGUC UUCA GGUAUAU@LB@3'-AUU...ACAGG G A UACAA...CAA-5'@LB@ | |@LB@ 138 102@LB@@LB@@LB@Energy -9.77 kcal/mol@LB@Hybridization Energy -16.4629 kcal/mol@LB@Unfolding Energy - mRNA 4.99509 kcal/mol@LB@Unfolding Energy - sRNA 3.33887 kcal/mol@LB@@LB@Position - mRNA 136 -- 162@LB@Position - sRNA 103 -- 137@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 123 |
0.005649 |
0.1741 |
bsu37530 |
ywhC |
-10.56 |
0.250674 |
18 -- 29 |
105 -- 116 |
zinc metalloprotease YwhC |
|
_____________17___________30\n_____________|____________|\n5'-GUU...GCUUC____________GACAA...UCG-3'\n______________UGUUACUUUCUC\n______________|||:||||||||\n______________ACAGUGAAAGAG\n3'-AUU...UUACA____________UAGAA...CAA-5'\n_____________|____________|\n_____________117__________104\n |
23 -- 29 |
105 -- 111 |
-16.5612 kcal/mol |
1.98074 kcal/mol |
5.11978 kcal/mol |
17 30@LB@ | |@LB@5'-GUU...GCUUC GACAA...UCG-3'@LB@ UGUUACUUUCUC@LB@ |||:||||||||@LB@ ACAGUGAAAGAG@LB@3'-AUU...UUACA UAGAA...CAA-5'@LB@ | |@LB@ 117 104@LB@@LB@@LB@Energy -10.56 kcal/mol@LB@Hybridization Energy -16.5612 kcal/mol@LB@Unfolding Energy - mRNA 1.98074 kcal/mol@LB@Unfolding Energy - sRNA 5.11978 kcal/mol@LB@@LB@Position - mRNA 18 -- 29@LB@Position - sRNA 105 -- 116@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 130 |
0.005995 |
0.1741 |
bsu19660 |
yozD |
-10.24 |
0.285413 |
18 -- 51 |
105 -- 135 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________17_________________________________52\n_____________|__________________________________|\n5'-CAC...CUUUG____UUUU_____U______AUCAAAU_______CGUCC...UGU-3'\n______________AGCG____UUCCA_UAUGAU_______CUUUCUC\n______________|||:____|||||_|||:||_______|||||||\n______________UCGU____AAGGU_AUAUUA_______GAAAGAG\n3'-AUU...AGGCG____UC______________CAACAGU_______UAGAA...CAA-5'\n_____________|__________________________________|\n_____________136________________________________104\n |
45 -- 51 |
105 -- 111 |
-19.2876 kcal/mol |
2.67508 kcal/mol |
6.61557 kcal/mol |
17 52@LB@ | |@LB@5'-CAC...CUUUG UUUU U AUCAAAU CGUCC...UGU-3'@LB@ AGCG UUCCA UAUGAU CUUUCUC@LB@ |||: ||||| |||:|| |||||||@LB@ UCGU AAGGU AUAUUA GAAAGAG@LB@3'-AUU...AGGCG UC CAACAGU UAGAA...CAA-5'@LB@ | |@LB@ 136 104@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -19.2876 kcal/mol@LB@Unfolding Energy - mRNA 2.67508 kcal/mol@LB@Unfolding Energy - sRNA 6.61557 kcal/mol@LB@@LB@Position - mRNA 18 -- 51@LB@Position - sRNA 105 -- 135@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 159 |
0.007366 |
0.1751 |
bsu06380 |
yebC |
-16.67 |
0.014812 |
77 -- 156 |
105 -- 185 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________76__________________________________________________________________________157\n_____________|___________________________________________________________________________|\n5'-ACC...CAUGC_______AA___G__AAU________________AGGAA___UUC___A__U____UAA________________GCUGA...CGA-3'\n______________UUUACUU___AU_GA___GAC_UUGAC__UGUUC_____GGC___GGU_CC_UAUA___GUGUU_UUAUUUUCUU\n______________||||||:___||_:|___|||_|||||__|||:|_____:||___:||_||_||||___:||||_|:|:|||||:\n______________AAAUGAG___UA_UU___CUG_AACUG__ACAGG_____UCG___UCA_GG_AUAU___UACAA_AGUGAAAGAG\n3'-AUU...AAAAA_______AAG__G__GUC___U_____UA_____CG______U_____A__U____________C__________UAGAA...CAA-5'\n_____________|___________________________________________________________________________|\n_____________186_________________________________________________________________________104\n |
87 -- 93 |
165 -- 171 |
-36.2834 kcal/mol |
12.0661 kcal/mol |
14.3768 kcal/mol |
76 157@LB@ | |@LB@5'-ACC...CAUGC AA G AAU AGGAA UUC A U UAA GCUGA...CGA-3'@LB@ UUUACUU AU GA GAC UUGAC UGUUC GGC GGU CC UAUA GUGUU UUAUUUUCUU@LB@ ||||||: || :| ||| ||||| |||:| :|| :|| || |||| :|||| |:|:|||||:@LB@ AAAUGAG UA UU CUG AACUG ACAGG UCG UCA GG AUAU UACAA AGUGAAAGAG@LB@3'-AUU...AAAAA AAG G GUC U UA CG U A U C UAGAA...CAA-5'@LB@ | |@LB@ 186 104@LB@@LB@@LB@Energy -16.67 kcal/mol@LB@Hybridization Energy -36.2834 kcal/mol@LB@Unfolding Energy - mRNA 12.0661 kcal/mol@LB@Unfolding Energy - sRNA 14.3768 kcal/mol@LB@@LB@Position - mRNA 77 -- 156@LB@Position - sRNA 105 -- 185@LB@Position Seed - mRNA 87 -- 93@LB@Position Seed - sRNA 165 -- 171@LB@ |
| 45 |
0.001428 |
0.1206 |
bsu27290 |
yrzA |
-10.49 |
0.257926 |
1 -- 17 |
111 -- 136 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________0_________________18\n_____________|_________________|\n_______5'-CGGC_________________ACUCC...ACA-3'\n______________CCAUAU____UUGUCAC\n______________||||||____|||||||\n______________GGUAUA____AACAGUG\n3'-AUU...UUCAA______UUAC_______AAAGA...CAA-5'\n_____________|_________________|\n_____________137_______________110\n |
11 -- 17 |
111 -- 117 |
-14.7924 kcal/mol |
0.950105 kcal/mol |
3.76381 kcal/mol |
0 18@LB@ | |@LB@ 5'-CGGC ACUCC...ACA-3'@LB@ CCAUAU UUGUCAC@LB@ |||||| |||||||@LB@ GGUAUA AACAGUG@LB@3'-AUU...UUCAA UUAC AAAGA...CAA-5'@LB@ | |@LB@ 137 110@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -14.7924 kcal/mol@LB@Unfolding Energy - mRNA 0.950105 kcal/mol@LB@Unfolding Energy - sRNA 3.76381 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 111 -- 136@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 137 |
0.006339 |
0.1741 |
bsu39320 |
yxiB |
-11.20 |
0.191268 |
188 -- 222 |
111 -- 147 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________187_________________________223\n_____________|___________________________|\n5'-UAA...GUGUU______UGCAUA___A___________AUUCG...AAA-3'\n______________GCGGCA______GUG_AAUGUUGUUAC\n______________||:|||______:|:_||||||||:||\n______________CGUCGU______UAU_UUACAACAGUG\n3'-AUU...ACAGG______UCAAGG___A___________AAAGA...CAA-5'\n_____________|___________________________|\n_____________148_________________________110\n |
216 -- 222 |
111 -- 117 |
-23.3412 kcal/mol |
7.76229 kcal/mol |
5.28835 kcal/mol |
187 223@LB@ | |@LB@5'-UAA...GUGUU UGCAUA A AUUCG...AAA-3'@LB@ GCGGCA GUG AAUGUUGUUAC@LB@ ||:||| :|: ||||||||:||@LB@ CGUCGU UAU UUACAACAGUG@LB@3'-AUU...ACAGG UCAAGG A AAAGA...CAA-5'@LB@ | |@LB@ 148 110@LB@@LB@@LB@Energy -11.20 kcal/mol@LB@Hybridization Energy -23.3412 kcal/mol@LB@Unfolding Energy - mRNA 7.76229 kcal/mol@LB@Unfolding Energy - sRNA 5.28835 kcal/mol@LB@@LB@Position - mRNA 188 -- 222@LB@Position - sRNA 111 -- 147@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 166 |
0.007737 |
0.1751 |
bsu10770 |
wprA |
-9.60 |
0.364837 |
176 -- 232 |
111 -- 164 |
cell wall-associated protease |
|
_____________175_________________________________________________________233\n_____________|___________________________________________________________|\n5'-CUG...GGAGA_______UUUU______G_GAUA____GAAA_____AAAA_____GCUCG______G__GGCAG...GCG-3'\n______________UUCAUUA____GCAGGA_G____ACAU____CGCAG_____UUCA______GUUGU_GC\n______________|||||:|____:|||||_:____||||____|||||_____|:||______|||||_:|\n______________AAGUAGU____UGUCCU_U____UGUA____GCGUC_____AGGU______CAACA_UG\n3'-AUU...AUGAG_________________G_AAC_____ACAG_____GUUCA____AUAUUA_____G__AAAGA...CAA-5'\n_____________|___________________________________________________________|\n_____________165_________________________________________________________110\n |
176 -- 182 |
158 -- 164 |
-36.1081 kcal/mol |
15.1078 kcal/mol |
11.9485 kcal/mol |
175 233@LB@ | |@LB@5'-CUG...GGAGA UUUU G GAUA GAAA AAAA GCUCG G GGCAG...GCG-3'@LB@ UUCAUUA GCAGGA G ACAU CGCAG UUCA GUUGU GC@LB@ |||||:| :||||| : |||| ||||| |:|| ||||| :|@LB@ AAGUAGU UGUCCU U UGUA GCGUC AGGU CAACA UG@LB@3'-AUU...AUGAG G AAC ACAG GUUCA AUAUUA G AAAGA...CAA-5'@LB@ | |@LB@ 165 110@LB@@LB@@LB@Energy -9.60 kcal/mol@LB@Hybridization Energy -36.1081 kcal/mol@LB@Unfolding Energy - mRNA 15.1078 kcal/mol@LB@Unfolding Energy - sRNA 11.9485 kcal/mol@LB@@LB@Position - mRNA 176 -- 232@LB@Position - sRNA 111 -- 164@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 125 |
0.005817 |
0.1741 |
bsu26980 |
yraE |
-11.00 |
0.208718 |
35 -- 90 |
112 -- 185 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________34________________________________________________________91\n_____________|_________________________________________________________|\n5'-UAC...UUUGA___AG______________AA_______A____GGA_____________________AGCCA...CUG-3'\n______________ACU___UAUCGACAGG_CA_____AUUG_UCCG_______________GAUGUUGUC\n______________|||___:|||:|||||_||_____||||_||||_______________:||||||||\n______________UGA___GUAGUUGUCC_GU_____UAAC_AGGC_______________UUACAACAG\n3'-AUU...AAAAA___GAA__________U__AACUG_________GUCGUUCAAGGUAUA_________UGAAA...CAA-5'\n_____________|_________________________________________________________|\n_____________186_______________________________________________________111\n |
83 -- 89 |
113 -- 119 |
-30.5681 kcal/mol |
8.54797 kcal/mol |
10.8348 kcal/mol |
34 91@LB@ | |@LB@5'-UAC...UUUGA AG AA A GGA AGCCA...CUG-3'@LB@ ACU UAUCGACAGG CA AUUG UCCG GAUGUUGUC@LB@ ||| :|||:||||| || |||| |||| :||||||||@LB@ UGA GUAGUUGUCC GU UAAC AGGC UUACAACAG@LB@3'-AUU...AAAAA GAA U AACUG GUCGUUCAAGGUAUA UGAAA...CAA-5'@LB@ | |@LB@ 186 111@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -30.5681 kcal/mol@LB@Unfolding Energy - mRNA 8.54797 kcal/mol@LB@Unfolding Energy - sRNA 10.8348 kcal/mol@LB@@LB@Position - mRNA 35 -- 90@LB@Position - sRNA 112 -- 185@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 113 -- 119@LB@ |
| 67 |
0.002241 |
0.1242 |
bsu04040 |
ycsE |
-12.95 |
0.087665 |
83 -- 153 |
117 -- 184 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pe: enzyme' |
|
_____________82________________________________________________________________________________154\n_____________|_________________________________________________________________________________|\n________5'-AUC____C____AAG________C__GG___UUU__U___G_________GC______________GUCAU______UC_____CACCG...GCA-3'\n______________GGAU_GGUU___UCGUUGUG_UC__GGU___CA_UUG__UUUGUUUU__UGUUUUU__UUUCU_____GUUUGA__AC_CU\n______________|:||_||:|___||||||::_||__||:___||_|:|__::::|:|:__|:||:::__|||||_____:|||||__||_||\n______________CUUA_CCGA___AGCAACGU_AG__CCG___GU_AGC__GGGUAGAG__AUAAGGG__AAAGA_____UAAACU__UG_GA\n3'-AUU...CAUUA____________________U__AA___UUU__U___AU________A________GU_____AACUU______UU__C__AUUAA...CAA-5'\n_____________|_________________________________________________________________________________|\n_____________185_______________________________________________________________________________116\n |
52 -- 58 |
40 -- 46 |
-38.9686 kcal/mol |
11.5965 kcal/mol |
18.2899 kcal/mol |
82 154@LB@ | |@LB@ 5'-AUC C AAG C GG UUU U G GC GUCAU UC CACCG...GCA-3'@LB@ GGAU GGUU UCGUUGUG UC GGU CA UUG UUUGUUUU UGUUUUU UUUCU GUUUGA AC CU@LB@ |:|| ||:| ||||||:: || ||: || |:| ::::|:|: |:||::: ||||| :||||| || ||@LB@ CUUA CCGA AGCAACGU AG CCG GU AGC GGGUAGAG AUAAGGG AAAGA UAAACU UG GA@LB@3'-AUU...CAUUA U AA UUU U AU A GU AACUU UU C AUUAA...CAA-5'@LB@ | |@LB@ 185 116@LB@@LB@@LB@Energy -12.95 kcal/mol@LB@Hybridization Energy -38.9686 kcal/mol@LB@Unfolding Energy - mRNA 11.5965 kcal/mol@LB@Unfolding Energy - sRNA 18.2899 kcal/mol@LB@@LB@Position - mRNA 83 -- 153@LB@Position - sRNA 117 -- 184@LB@Position Seed - mRNA 52 -- 58@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 18 |
0.0005418 |
0.1011 |
bsu07080 |
yesZ |
-22.48 |
0.000806 |
260 -- 300 |
118 -- 160 |
beta-galactosidase YesZ |
|
_____________259_______________301\n_____________|_________________|\n5'-GAU...ACGAU_________________UCAUG...AUG-3'\n______________UCAGCAGGACAUUGACA\n______________|||:|||||||||||||\n______________AGUUGUCCUGUAACUGU\n3'-AUU...GAAGU_________________AACAG...CAA-5'\n_____________|_________________|\n_____________161_______________117\n |
270 -- 276 |
144 -- 150 |
-28.5551 kcal/mol |
3.65993 kcal/mol |
6.86594 kcal/mol |
259 301@LB@ | |@LB@5'-GAU...ACGAU UCAUG...AUG-3'@LB@ UCAGCAGGACAUUGACA@LB@ |||:|||||||||||||@LB@ AGUUGUCCUGUAACUGU@LB@3'-AUU...GAAGU AACAG...CAA-5'@LB@ | |@LB@ 161 117@LB@@LB@@LB@Energy -22.48 kcal/mol@LB@Hybridization Energy -28.5551 kcal/mol@LB@Unfolding Energy - mRNA 3.65993 kcal/mol@LB@Unfolding Energy - sRNA 6.86594 kcal/mol@LB@@LB@Position - mRNA 260 -- 300@LB@Position - sRNA 118 -- 160@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 144 -- 150@LB@ |
