| 92 |
0.003598 |
0.1515 |
bsu22200 |
cotD |
-17.29 |
0.010920 |
45 -- 158 |
37 -- 130 |
spore coat protein D |
|
_____________44________________________________________________________________________________________________________________________159\n_____________|_________________________________________________________________________________________________________________________|\n5'-AUU...UACUU_____U___U___GUAUUU___A________GU_________CUU___A__CA_____AA_____CACUUUUAAAAUAG_____U___UCAG_____________________________AACUU...ACC-3'\n______________GUUCU_UGU_GUG______UUG_UUGCUUUU__GUUUUCAAA___UAA_GA___GCUU__UUGCA______________GUCUU_GCA____AACA__UGUACCC____CUUAUUUUUCAU\n______________||||:_|:|_:||______|||_|::||||:__:|:||||||___|||_||___||||__|||||______________:||||_|||____||||__:||||||____||||||:::|||\n______________CAAGG_AUA_UAC______AAC_AGUGAAAG__UAGAAGUUU___AUU_CU___CGAA__AACGU______________UAGAA_CGU____UUGU__GCAUGGG____GAAUAAGGGGUA\n3'-AUU...UCGUU_____U___U_____________________AG_________C_____A__UAC____GC________________________C___________UA_______UAGA____________AAGAA...CAA-5'\n_____________|_________________________________________________________________________________________________________________________|\n_____________131_______________________________________________________________________________________________________________________36\n |
147 -- 153 |
42 -- 48 |
-55.4941 kcal/mol |
20.5826 kcal/mol |
23.6024 kcal/mol |
44 159@LB@ | |@LB@5'-AUU...UACUU U U GUAUUU A GU CUU A CA AA CACUUUUAAAAUAG U UCAG AACUU...ACC-3'@LB@ GUUCU UGU GUG UUG UUGCUUUU GUUUUCAAA UAA GA GCUU UUGCA GUCUU GCA AACA UGUACCC CUUAUUUUUCAU@LB@ ||||: |:| :|| ||| |::||||: :|:|||||| ||| || |||| ||||| :|||| ||| |||| :|||||| ||||||:::|||@LB@ CAAGG AUA UAC AAC AGUGAAAG UAGAAGUUU AUU CU CGAA AACGU UAGAA CGU UUGU GCAUGGG GAAUAAGGGGUA@LB@3'-AUU...UCGUU U U AG C A UAC GC C UA UAGA AAGAA...CAA-5'@LB@ | |@LB@ 131 36@LB@@LB@@LB@Energy -17.29 kcal/mol@LB@Hybridization Energy -55.4941 kcal/mol@LB@Unfolding Energy - mRNA 20.5826 kcal/mol@LB@Unfolding Energy - sRNA 23.6024 kcal/mol@LB@@LB@Position - mRNA 45 -- 158@LB@Position - sRNA 37 -- 130@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 110 |
0.004746 |
0.1671 |
bsu09940 |
yhaL |
-13.90 |
0.056226 |
141 -- 159 |
37 -- 56 |
sporulation protein YhaL |
|
_____________140______________________________________160\n_____________|________________________________________|\n5'-GAA...UUUUU____________GUA___U____U___A_GCA________AGCGC...ACC-3'\n______________UCCGUGGUGGGU___UCU_UGUA_UGU_G___UCAUUUUC\n______________||||::|:::||___||:_|:||_|||_|___|||:||||\n______________AGGCGUCGUUCA___AGG_AUAU_ACA_C___AGUGAAAG\n3'-AUU...GUAAC__________________U____U___A____________AGUAG...CAA-5'\n_____________|________________________________________|\n_____________57_______________________________________36\n |
245 -- 251 |
107 -- 113 |
-27.9954 kcal/mol |
7.44184 kcal/mol |
9.97721 kcal/mol |
140 160@LB@ | |@LB@5'-GAA...UUUUU GUA U U A GCA AGCGC...ACC-3'@LB@ UCCGUGGUGGGU UCU UGUA UGU G UCAUUUUC@LB@ ||||::|:::|| ||: |:|| ||| | |||:||||@LB@ AGGCGUCGUUCA AGG AUAU ACA C AGUGAAAG@LB@3'-AUU...GUAAC U U A AGUAG...CAA-5'@LB@ | |@LB@ 57 36@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -27.9954 kcal/mol@LB@Unfolding Energy - mRNA 7.44184 kcal/mol@LB@Unfolding Energy - sRNA 9.97721 kcal/mol@LB@@LB@Position - mRNA 141 -- 159@LB@Position - sRNA 37 -- 56@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 107 -- 113@LB@ |
| 120 |
0.005453 |
0.1741 |
bsu38750 |
cydB |
-16.17 |
0.018868 |
36 -- 121 |
37 -- 136 |
cytochrome d ubiquinol oxidase subunit 2 |
|
_____________35____________________________________________________________________________________________________________122\n_____________|_____________________________________________________________________________________________________________|\n5'-AAU...CGUAA___________________________C__AA_____GCA_____U______UAC___AU__________________________________U________A_____GAGAU...CCC-3'\n______________CAGCGGGUUCC________UUGUUAUU_UC__UCAUC___UUCGG_GUGAUG___AUG____________UCUUGGU__GCA__C_UGCUUGUC_UCUUGUUU_UCCGU\n______________||||::|||||________||||:|:|_||__|||||___|||::_||:|||___|||____________||||||:__:||__|_|:|:::||_||||:||:_:||:|\n______________GUCGUUCAAGG________AACAGUGA_AG__AGUAG___AAGUU_CAUUAC___UAC____________AGAACCG__UGU__G_AUGGGUAG_AGAAUAAG_GGGUA\n3'-AUU...CAGGC___________UAUAUUAC________A_________________U______U_____CGAAGCAACGUU_______UU___UA_C_______________________AAGAA...CAA-5'\n_____________|_____________________________________________________________________________________________________________|\n_____________137___________________________________________________________________________________________________________36\n |
109 -- 115 |
42 -- 48 |
-60.5971 kcal/mol |
23.6183 kcal/mol |
24.7809 kcal/mol |
35 122@LB@ | |@LB@5'-AAU...CGUAA C AA GCA U UAC AU U A GAGAU...CCC-3'@LB@ CAGCGGGUUCC UUGUUAUU UC UCAUC UUCGG GUGAUG AUG UCUUGGU GCA C UGCUUGUC UCUUGUUU UCCGU@LB@ ||||::||||| ||||:|:| || ||||| |||:: ||:||| ||| ||||||: :|| | |:|:::|| ||||:||: :||:|@LB@ GUCGUUCAAGG AACAGUGA AG AGUAG AAGUU CAUUAC UAC AGAACCG UGU G AUGGGUAG AGAAUAAG GGGUA@LB@3'-AUU...CAGGC UAUAUUAC A U U CGAAGCAACGUU UU UA C AAGAA...CAA-5'@LB@ | |@LB@ 137 36@LB@@LB@@LB@Energy -16.17 kcal/mol@LB@Hybridization Energy -60.5971 kcal/mol@LB@Unfolding Energy - mRNA 23.6183 kcal/mol@LB@Unfolding Energy - sRNA 24.7809 kcal/mol@LB@@LB@Position - mRNA 36 -- 121@LB@Position - sRNA 37 -- 136@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 136 |
0.006329 |
0.1741 |
bsu10210 |
yhfF |
-9.29 |
0.409483 |
117 -- 143 |
37 -- 59 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________116______________144\n_____________|________________|\n5'-AUA...GACGC_______GCA______CGCAC...CCA-3'\n______________UUCCCAU___UUUGAA\n______________|:|||||___||||||\n______________AGGGGUA___AAACUU\n3'-AUU...GAAUA_______AAG______UAAAC...CAA-5'\n_____________|________________|\n_____________60_______________36\n |
101 -- 107 |
37 -- 43 |
-14.7282 kcal/mol |
3.20386 kcal/mol |
5.02573 kcal/mol |
116 144@LB@ | |@LB@5'-AUA...GACGC GCA CGCAC...CCA-3'@LB@ UUCCCAU UUUGAA@LB@ |:||||| ||||||@LB@ AGGGGUA AAACUU@LB@3'-AUU...GAAUA AAG UAAAC...CAA-5'@LB@ | |@LB@ 60 36@LB@@LB@@LB@Energy -9.29 kcal/mol@LB@Hybridization Energy -14.7282 kcal/mol@LB@Unfolding Energy - mRNA 3.20386 kcal/mol@LB@Unfolding Energy - sRNA 5.02573 kcal/mol@LB@@LB@Position - mRNA 117 -- 143@LB@Position - sRNA 37 -- 59@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 148 |
0.006782 |
0.1741 |
bsu14050 |
ykuE |
-11.57 |
0.163142 |
1 -- 16 |
37 -- 54 |
metallophosphoesterase |
|
______________1__________________________17\n______________|__________________________|\n___________5'-_____G____________GC_GC____CCCGC...AUC-3'\n______________CCAUC_CU__UUUCCCAU__C__UUGA\n______________|||||_||__||:|||||__|__||||\n______________GGUAG_GA__AAGGGGUA__G__AACU\n3'-AUU...GCAUG_____A__AU________AA_A_____UUAAA...CAA-5'\n_____________|___________________________|\n_____________55__________________________36\n |
10 -- 16 |
37 -- 43 |
-20.8225 kcal/mol |
1.63511 kcal/mol |
9.4766 kcal/mol |
1 17@LB@ | |@LB@ 5'- G GC GC CCCGC...AUC-3'@LB@ CCAUC CU UUUCCCAU C UUGA@LB@ ||||| || ||:||||| | ||||@LB@ GGUAG GA AAGGGGUA G AACU@LB@3'-AUU...GCAUG A AU AA A UUAAA...CAA-5'@LB@ | |@LB@ 55 36@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -20.8225 kcal/mol@LB@Unfolding Energy - mRNA 1.63511 kcal/mol@LB@Unfolding Energy - sRNA 9.4766 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 178 |
0.008149 |
0.1751 |
bsu39610 |
yxeB |
-9.68 |
0.354141 |
1 -- 23 |
37 -- 58 |
iron(3+)-hydroxamate-binding protein YxeB |
bsu33320 |
_____________0_______24\n_____________|_______|\n5'-GCA...UGUAA_______AUUUU...CUG-3'\n______________UUCCCAU\n______________|:|||||\n______________AGGGGUA\n3'-AUU...GAAUA_______AAGAA...CAA-5'\n_____________|_______|\n_____________59______36\n |
17 -- 23 |
37 -- 43 |
-9.25042 kcal/mol |
0.0358808 kcal/mol |
2.53706 kcal/mol |
0 24@LB@ | |@LB@5'-GCA...UGUAA AUUUU...CUG-3'@LB@ UUCCCAU@LB@ |:|||||@LB@ AGGGGUA@LB@3'-AUU...GAAUA AAGAA...CAA-5'@LB@ | |@LB@ 59 36@LB@@LB@@LB@Energy -9.68 kcal/mol@LB@Hybridization Energy -9.25042 kcal/mol@LB@Unfolding Energy - mRNA 0.0358808 kcal/mol@LB@Unfolding Energy - sRNA 2.53706 kcal/mol@LB@@LB@Position - mRNA 1 -- 23@LB@Position - sRNA 37 -- 58@LB@Position Seed - mRNA 17 -- 23@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 184 |
0.008461 |
0.1751 |
bsu09120 |
dgcK |
-13.04 |
0.083992 |
38 -- 184 |
37 -- 168 |
diguanylate cyclase |
|
_____________37______________________________________185\n_____________|_______________________________________|\n5'-CAU...AAGUC___AA_______AAACUCUCCCUUCAA____________UACUA...GCA-3'\n______________UCC__AGCAGGU________________AUUUUCUCAUU\n______________|||__||||:||________________|:||||||||:\n______________AGG__UCGUUCA________________UGAAAGAGUAG\n3'-AUU...GUAAC___CG_______AGGUAUAUUACAACAG___________AAGUU...CAA-5'\n_____________|_______________________________________|\n_____________169_____________________________________36\n |
55 -- 61 |
102 -- 108 |
-22.9941 kcal/mol |
2.75938 kcal/mol |
10.7301 kcal/mol |
37 185@LB@ | |@LB@5'-CAU...AAGUC AA AAACUCUCCCUUCAA UACUA...GCA-3'@LB@ UCC AGCAGGU AUUUUCUCAUU@LB@ ||| ||||:|| |:||||||||:@LB@ AGG UCGUUCA UGAAAGAGUAG@LB@3'-AUU...GUAAC CG AGGUAUAUUACAACAG AAGUU...CAA-5'@LB@ | |@LB@ 169 36@LB@@LB@@LB@Energy -13.04 kcal/mol@LB@Hybridization Energy -22.9941 kcal/mol@LB@Unfolding Energy - mRNA 2.75938 kcal/mol@LB@Unfolding Energy - sRNA 10.7301 kcal/mol@LB@@LB@Position - mRNA 38 -- 184@LB@Position - sRNA 37 -- 168@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 1 |
1.123e-05 |
0.04352 |
bsu13990 |
kinA |
-26.46 |
0.000103 |
182 -- 297 |
38 -- 181 |
sporulation kinase A |
bsu14490 bsu13530 |
_____________181___________________________________________________________________________________________________________________________298\n_____________|_____________________________________________________________________________________________________________________________|\n5'-CUA...GAGGG_____GUGG________________________________________________AA______________CAAAAACCGAUA___A_____________CUC____G____G____A_____AUAUC...ACU-3'\n______________AUUCU_____AACAGGAUA___________CGCAGCA_______________UGUUA____C_CA_CUUCAAA____________UUC__UGCAGUCUUGGC___UAAU_G_AC_CAUC_UUUAU\n______________|:|||_____|||||||:|___________|||||||_______________|||:|____|_||_|||||||____________|||__||||:|||||||___:|||_|_||_||||_:||||\n______________UGAGA_____UUGUCCUGU___________GCGUCGU_______________ACAGU____G_GU_GAAGUUU____________AAG__ACGUUAGAACCG___GUUA_C_UG_GUAG_GAAUA\n3'-AUU...AAAAA_____AGUAG_________AACUGUAACAG_______UCAAGGUAUAUUACA_____GAAA_A__A_______CAUUACUUACCG___CA____________UUU____G_A__G____A_____AGGGG...CAA-5'\n_____________|_____________________________________________________________________________________________________________________________|\n_____________182___________________________________________________________________________________________________________________________37\n |
259 -- 265 |
67 -- 73 |
-65.6544 kcal/mol |
17.7451 kcal/mol |
29.7341 kcal/mol |
181 298@LB@ | |@LB@5'-CUA...GAGGG GUGG AA CAAAAACCGAUA A CUC G G A AUAUC...ACU-3'@LB@ AUUCU AACAGGAUA CGCAGCA UGUUA C CA CUUCAAA UUC UGCAGUCUUGGC UAAU G AC CAUC UUUAU@LB@ |:||| |||||||:| ||||||| |||:| | || ||||||| ||| ||||:||||||| :||| | || |||| :||||@LB@ UGAGA UUGUCCUGU GCGUCGU ACAGU G GU GAAGUUU AAG ACGUUAGAACCG GUUA C UG GUAG GAAUA@LB@3'-AUU...AAAAA AGUAG AACUGUAACAG UCAAGGUAUAUUACA GAAA A A CAUUACUUACCG CA UUU G A G A AGGGG...CAA-5'@LB@ | |@LB@ 182 37@LB@@LB@@LB@Energy -26.46 kcal/mol@LB@Hybridization Energy -65.6544 kcal/mol@LB@Unfolding Energy - mRNA 17.7451 kcal/mol@LB@Unfolding Energy - sRNA 29.7341 kcal/mol@LB@@LB@Position - mRNA 182 -- 297@LB@Position - sRNA 38 -- 181@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 21 |
0.0005858 |
0.1011 |
bsu27120 |
sigV |
-13.38 |
0.071859 |
12 -- 26 |
38 -- 54 |
RNA polymerase sigma factor SigV |
|
______________12__________________________27\n______________|___________________________|\n___________5'-______GUUUG_________________AAAAC...AAG-3'\n______________AAUAAU_____UCAUCUCUUA__CCCCA\n______________||:|||_____:|||||||||__|||||\n______________UUGUUA_____GGUAGAGAAU__GGGGU\n3'-AUU...ACCGU______GCAUG__________AA_____AAAGA...CAA-5'\n_____________|____________________________|\n_____________55___________________________37\n |
15 -- 21 |
45 -- 51 |
-23.0819 kcal/mol |
1.75763 kcal/mol |
10.671 kcal/mol |
12 27@LB@ | |@LB@ 5'- GUUUG AAAAC...AAG-3'@LB@ AAUAAU UCAUCUCUUA CCCCA@LB@ ||:||| :||||||||| |||||@LB@ UUGUUA GGUAGAGAAU GGGGU@LB@3'-AUU...ACCGU GCAUG AA AAAGA...CAA-5'@LB@ | |@LB@ 55 37@LB@@LB@@LB@Energy -13.38 kcal/mol@LB@Hybridization Energy -23.0819 kcal/mol@LB@Unfolding Energy - mRNA 1.75763 kcal/mol@LB@Unfolding Energy - sRNA 10.671 kcal/mol@LB@@LB@Position - mRNA 12 -- 26@LB@Position - sRNA 38 -- 54@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 83 |
0.002931 |
0.1368 |
bsu18650 |
pelB |
-17.38 |
0.010445 |
17 -- 103 |
38 -- 142 |
pectin lyase |
|
_____________16________________________________________________________________________________________________________________104\n_____________|_________________________________________________________________________________________________________________|\n5'-AGG...UAAGC_________U_______G_____UCAA____________CUA_________________________A___U____UU__________________________AGUAA____ACAUU...CAA-3'\n______________UG_CUGCAG_CAA___C_GUGUA______UC_CUUUCUU___UUUCAA______________UUGUU_GUA_GUCU__UGGC__GCAAUC___UCUAUUUUUUA_____CCCA\n______________||_|:||||_|||___|_:|:||______||_||||||:___:|||||______________|:|||_|:|_:|||__||||__:|||||___:|:||:|:|||_____||||\n______________AC_GGCGUC_GUU___G_UAUAU______AG_GAAAGAG___GAAGUU______________AGCAA_CGU_UAGA__ACCG__UGUUAG___GGGUAGAGAAU_____GGGU\n3'-AUU...CUGUA__A__________CAA_G_____UACAAC__U_______UA_______UCAUUACUUACCGA____________________UU______CAU___________AAG______AAAGA...CAA-5'\n_____________|_________________________________________________________________________________________________________________|\n_____________143_______________________________________________________________________________________________________________37\n |
42 -- 48 |
105 -- 111 |
-52.303 kcal/mol |
11.0023 kcal/mol |
27.6117 kcal/mol |
16 104@LB@ | |@LB@5'-AGG...UAAGC U G UCAA CUA A U UU AGUAA ACAUU...CAA-3'@LB@ UG CUGCAG CAA C GUGUA UC CUUUCUU UUUCAA UUGUU GUA GUCU UGGC GCAAUC UCUAUUUUUUA CCCA@LB@ || |:|||| ||| | :|:|| || ||||||: :||||| |:||| |:| :||| |||| :||||| :|:||:|:||| ||||@LB@ AC GGCGUC GUU G UAUAU AG GAAAGAG GAAGUU AGCAA CGU UAGA ACCG UGUUAG GGGUAGAGAAU GGGU@LB@3'-AUU...CUGUA A CAA G UACAAC U UA UCAUUACUUACCGA UU CAU AAG AAAGA...CAA-5'@LB@ | |@LB@ 143 37@LB@@LB@@LB@Energy -17.38 kcal/mol@LB@Hybridization Energy -52.303 kcal/mol@LB@Unfolding Energy - mRNA 11.0023 kcal/mol@LB@Unfolding Energy - sRNA 27.6117 kcal/mol@LB@@LB@Position - mRNA 17 -- 103@LB@Position - sRNA 38 -- 142@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 105 -- 111@LB@ |
