| 153 |
0.007014 |
0.1751 |
bsu10400 |
yhxC |
-16.66 |
0.014878 |
138 -- 167 |
138 -- 167 |
oxidoreductase |
|
_____________137____________________________168\n_____________|______________________________|\n5'-UCC...UCUAU________AC______AGCA__________AUAAC...CGA-3'\n______________CUUUUCAU__AUAGGA____GGCAUUGUUC\n______________||:|||||__|:||||____|:||||||:|\n______________GAGAAGUA__UGUCCU____CUGUAACAGG\n3'-AUU...AAAAU________GU______GUAA__________CGUCG...CAA-5'\n_____________|______________________________|\n_____________168____________________________137\n |
161 -- 167 |
138 -- 144 |
-29.0079 kcal/mol |
5.02609 kcal/mol |
7.37082 kcal/mol |
137 168@LB@ | |@LB@5'-UCC...UCUAU AC AGCA AUAAC...CGA-3'@LB@ CUUUUCAU AUAGGA GGCAUUGUUC@LB@ ||:||||| |:|||| |:||||||:|@LB@ GAGAAGUA UGUCCU CUGUAACAGG@LB@3'-AUU...AAAAU GU GUAA CGUCG...CAA-5'@LB@ | |@LB@ 168 137@LB@@LB@@LB@Energy -16.66 kcal/mol@LB@Hybridization Energy -29.0079 kcal/mol@LB@Unfolding Energy - mRNA 5.02609 kcal/mol@LB@Unfolding Energy - sRNA 7.37082 kcal/mol@LB@@LB@Position - mRNA 138 -- 167@LB@Position - sRNA 138 -- 167@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 138 -- 144@LB@ |
| 37 |
0.00122 |
0.1206 |
bsu31150 |
yubB |
-14.80 |
0.036692 |
271 -- 292 |
145 -- 165 |
undecaprenyl-diphosphatase |
|
_____________270____________________293\n_____________|______________________|\n5'-UAU...GCCGG____U____________A____CAUAGUAG-3'\n______________UUUC_UCGACAGGACAU_UGAU\n______________:|||_||:|||||||||_|||:\n______________GAAG_AGUUGUCCUGUA_ACUG\n3'-AUU...AAUGA____U_________________UAACA...CAA-5'\n_____________|______________________|\n_____________166____________________144\n |
281 -- 287 |
149 -- 155 |
-24.6442 kcal/mol |
4.4819 kcal/mol |
7.11846 kcal/mol |
270 293@LB@ | |@LB@5'-UAU...GCCGG U A CAUAGUAG-3'@LB@ UUUC UCGACAGGACAU UGAU@LB@ :||| ||:||||||||| |||:@LB@ GAAG AGUUGUCCUGUA ACUG@LB@3'-AUU...AAUGA U UAACA...CAA-5'@LB@ | |@LB@ 166 144@LB@@LB@@LB@Energy -14.80 kcal/mol@LB@Hybridization Energy -24.6442 kcal/mol@LB@Unfolding Energy - mRNA 4.4819 kcal/mol@LB@Unfolding Energy - sRNA 7.11846 kcal/mol@LB@@LB@Position - mRNA 271 -- 292@LB@Position - sRNA 145 -- 165@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 149 -- 155@LB@ |
| 188 |
0.008618 |
0.1751 |
bsu31720 |
degQ |
-9.47 |
0.382925 |
276 -- 300 |
147 -- 170 |
degradation enzyme regulation protein DegQ |
|
_____________275______________301\n_____________|________________|\n5'-GAA...AUUCA____GAAA________AAAGC...UUG-3'\n______________UUAC____CAUUAACA\n______________||||____|||:||||\n______________AAUG____GUAGUUGU\n3'-AUU...AAAAA____AGAA________CCUGU...CAA-5'\n_____________|________________|\n_____________171______________146\n |
285 -- 291 |
155 -- 161 |
-9.46814 kcal/mol |
0.995164 kcal/mol |
1.41808 kcal/mol |
275 301@LB@ | |@LB@5'-GAA...AUUCA GAAA AAAGC...UUG-3'@LB@ UUAC CAUUAACA@LB@ |||| |||:||||@LB@ AAUG GUAGUUGU@LB@3'-AUU...AAAAA AGAA CCUGU...CAA-5'@LB@ | |@LB@ 171 146@LB@@LB@@LB@Energy -9.47 kcal/mol@LB@Hybridization Energy -9.46814 kcal/mol@LB@Unfolding Energy - mRNA 0.995164 kcal/mol@LB@Unfolding Energy - sRNA 1.41808 kcal/mol@LB@@LB@Position - mRNA 276 -- 300@LB@Position - sRNA 147 -- 170@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 155 -- 161@LB@ |
| 111 |
0.004819 |
0.1671 |
bsu32120 |
yuzG |
-7.22 |
0.752372 |
165 -- 182 |
149 -- 166 |
Evidence 5: No homology to any previously reported sequences |
|
_____________164______________________________________183\n_____________|________________________________________|\n5'-UAG...UCUUU_____A_G_ACA__G__AAG__A________GAUG_____AAACG...UGG-3'\n______________AUGGC_U_G___UG_GA____G_GCGAAUAA____GCCCA\n______________|||||_|_|___||_:|____|_||:||:||____:||||\n______________UACCG_A_C___AC_UU____C_CGUUUGUU____UGGGU\n3'-AUU...UUACU_____A_G_A____G__AGAA__________AGCA_____AGAGA...CAA-5'\n_____________|________________________________________|\n_____________167______________________________________148\n |
192 -- 198 |
62 -- 68 |
-24.2214 kcal/mol |
8.24153 kcal/mol |
10.1588 kcal/mol |
164 183@LB@ | |@LB@5'-UAG...UCUUU A G ACA G AAG A GAUG AAACG...UGG-3'@LB@ AUGGC U G UG GA G GCGAAUAA GCCCA@LB@ ||||| | | || :| | ||:||:|| :||||@LB@ UACCG A C AC UU C CGUUUGUU UGGGU@LB@3'-AUU...UUACU A G A G AGAA AGCA AGAGA...CAA-5'@LB@ | |@LB@ 167 148@LB@@LB@@LB@Energy -7.22 kcal/mol@LB@Hybridization Energy -24.2214 kcal/mol@LB@Unfolding Energy - mRNA 8.24153 kcal/mol@LB@Unfolding Energy - sRNA 10.1588 kcal/mol@LB@@LB@Position - mRNA 165 -- 182@LB@Position - sRNA 149 -- 166@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 102 |
0.004212 |
0.1594 |
bsu10510 |
yhjH |
-17.94 |
0.007893 |
238 -- 265 |
152 -- 183 |
MarR family transcriptional regulator |
|
_____________237________________266\n_____________|__________________|\n5'-GCA...GCGGA____A_____________GAAAU...CUG-3'\n______________GCUC_UUCAUCAGCAGGA\n______________:|||_|||||||:|||||\n______________UGAG_AAGUAGUUGUCCU\n3'-AUU...AAAAA__________________GUAAC...CAA-5'\n_____________|__________________|\n_____________184________________151\n |
259 -- 265 |
152 -- 158 |
-25.8948 kcal/mol |
5.0064 kcal/mol |
6.06644 kcal/mol |
237 266@LB@ | |@LB@5'-GCA...GCGGA A GAAAU...CUG-3'@LB@ GCUC UUCAUCAGCAGGA@LB@ :||| |||||||:|||||@LB@ UGAG AAGUAGUUGUCCU@LB@3'-AUU...AAAAA GUAAC...CAA-5'@LB@ | |@LB@ 184 151@LB@@LB@@LB@Energy -17.94 kcal/mol@LB@Hybridization Energy -25.8948 kcal/mol@LB@Unfolding Energy - mRNA 5.0064 kcal/mol@LB@Unfolding Energy - sRNA 6.06644 kcal/mol@LB@@LB@Position - mRNA 238 -- 265@LB@Position - sRNA 152 -- 183@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 152 -- 158@LB@ |
| 47 |
0.001488 |
0.1206 |
bsu18480 |
proH |
-12.75 |
0.095888 |
85 -- 112 |
155 -- 184 |
pyrroline-5-carboxylate reductase 1 |
bsu23800 |
_____________84_______________113\n_____________|________________|\n5'-AGC...UUUUC____________U___UAUUU...CAG-3'\n______________UGCUUUUCAUUA_ACA\n______________|:||:|||||:|_|||\n______________AUGAGAAGUAGU_UGU\n3'-AUU...AAAAA________________CCUGU...CAA-5'\n_____________|________________|\n_____________185______________154\n |
102 -- 108 |
158 -- 164 |
-13.4777 kcal/mol |
3.19807 kcal/mol |
1.41616 kcal/mol |
84 113@LB@ | |@LB@5'-AGC...UUUUC U UAUUU...CAG-3'@LB@ UGCUUUUCAUUA ACA@LB@ |:||:|||||:| |||@LB@ AUGAGAAGUAGU UGU@LB@3'-AUU...AAAAA CCUGU...CAA-5'@LB@ | |@LB@ 185 154@LB@@LB@@LB@Energy -12.75 kcal/mol@LB@Hybridization Energy -13.4777 kcal/mol@LB@Unfolding Energy - mRNA 3.19807 kcal/mol@LB@Unfolding Energy - sRNA 1.41616 kcal/mol@LB@@LB@Position - mRNA 85 -- 112@LB@Position - sRNA 155 -- 184@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 107 |
0.004539 |
0.1633 |
bsu11020 |
yitK |
-8.14 |
0.595243 |
151 -- 171 |
155 -- 171 |
nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription |
|
_____________150___________________172\n_____________|_____________________|\n5'-ACC...UUCUC________ACUAUA_______CAUAA...AUG-3'\n______________UUUAUUCU______AUCAACA\n______________||||:|||______|||||||\n______________AAAUGAGA______UAGUUGU\n3'-AUU...AAAAA________AG___________CCUGU...CAA-5'\n_____________|_____________________|\n_____________172___________________154\n |
165 -- 171 |
155 -- 161 |
-10.3591 kcal/mol |
1.70839 kcal/mol |
1.4241 kcal/mol |
150 172@LB@ | |@LB@5'-ACC...UUCUC ACUAUA CAUAA...AUG-3'@LB@ UUUAUUCU AUCAACA@LB@ ||||:||| |||||||@LB@ AAAUGAGA UAGUUGU@LB@3'-AUU...AAAAA AG CCUGU...CAA-5'@LB@ | |@LB@ 172 154@LB@@LB@@LB@Energy -8.14 kcal/mol@LB@Hybridization Energy -10.3591 kcal/mol@LB@Unfolding Energy - mRNA 1.70839 kcal/mol@LB@Unfolding Energy - sRNA 1.4241 kcal/mol@LB@@LB@Position - mRNA 151 -- 171@LB@Position - sRNA 155 -- 171@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 155 -- 161@LB@ |
| 145 |
0.006739 |
0.1741 |
bsu12460 |
xlyB |
-11.80 |
0.146979 |
21 -- 64 |
155 -- 186 |
N-acetylmuramoyl-L-alanine amidase XlyB |
bsu12810 bsu25710 bsu25900 bsu21410 |
_____________20____________________________________65\n_____________|_____________________________________|\n5'-UCC...CCACA_____C_G_____AUUUCUC____CU_______A___UUAAA...CGG-3'\n______________UGCUC_U_AUUAA_______CGUU__CAUUGUC_GCA\n______________|:|||_|_||:||_______|:||__|||||||_|||\n______________AUGAG_A_UAGUU_______GUAA__GUAACAG_CGU\n3'-AUU...AAAAA_____A_G_____GUCCU______CU_______G___CGUUC...CAA-5'\n_____________|_____________________________________|\n_____________187___________________________________154\n |
54 -- 60 |
139 -- 145 |
-22.5299 kcal/mol |
4.58402 kcal/mol |
8.54622 kcal/mol |
20 65@LB@ | |@LB@5'-UCC...CCACA C G AUUUCUC CU A UUAAA...CGG-3'@LB@ UGCUC U AUUAA CGUU CAUUGUC GCA@LB@ |:||| | ||:|| |:|| ||||||| |||@LB@ AUGAG A UAGUU GUAA GUAACAG CGU@LB@3'-AUU...AAAAA A G GUCCU CU G CGUUC...CAA-5'@LB@ | |@LB@ 187 154@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -22.5299 kcal/mol@LB@Unfolding Energy - mRNA 4.58402 kcal/mol@LB@Unfolding Energy - sRNA 8.54622 kcal/mol@LB@@LB@Position - mRNA 21 -- 64@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 139 -- 145@LB@ |
| 187 |
0.008608 |
0.1751 |
bsu17960 |
yneK |
-9.96 |
0.317804 |
143 -- 172 |
155 -- 184 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________142___________173\n_____________|_____________|\n5'-AGU...AAUCU________AU___AUAUG...UAA-3'\n______________CUCUUCGU__ACA\n______________||||||:|__|||\n______________GAGAAGUA__UGU\n3'-AUU...AAAAU________GU___CCUGU...CAA-5'\n_____________|_____________|\n_____________185___________154\n |
161 -- 167 |
160 -- 166 |
-12.096 kcal/mol |
1.39305 kcal/mol |
1.41024 kcal/mol |
142 173@LB@ | |@LB@5'-AGU...AAUCU AU AUAUG...UAA-3'@LB@ CUCUUCGU ACA@LB@ ||||||:| |||@LB@ GAGAAGUA UGU@LB@3'-AUU...AAAAU GU CCUGU...CAA-5'@LB@ | |@LB@ 185 154@LB@@LB@@LB@Energy -9.96 kcal/mol@LB@Hybridization Energy -12.096 kcal/mol@LB@Unfolding Energy - mRNA 1.39305 kcal/mol@LB@Unfolding Energy - sRNA 1.41024 kcal/mol@LB@@LB@Position - mRNA 143 -- 172@LB@Position - sRNA 155 -- 184@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 160 -- 166@LB@ |
| 142 |
0.006636 |
0.1741 |
bsu22510 |
ypjC |
-12.74 |
0.096342 |
122 -- 155 |
156 -- 190 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________121_____________________________________________________________________________156\n_____________|_______________________________________________________________________________|\n5'-UAA...UCAUC_____A___GUUAGCUAAUU___AU___U______AA_____A_____C_____________C____UU__________CACUA...UGG-3'\n______________CUCUU_CAU___________CAG__ACG_UUGACA__UGUUU__AGCA_GUU________UG_UGUU__CUUUUUCAUC\n______________|||||_|||___________|||__||:_||||||__|||::__||||_|||________||_|||:__||||:|||||\n______________GAGAA_GUA___________GUC__UGU_AACUGU__ACAGG__UCGU_CAA________AC_ACAG__GAAAGAGUAG\n3'-AUU...AAAAU_________GUU___________C___________A______CG____U___GGUAUAUU__A____U___________AAGUU...CAA-5'\n_____________|_______________________________________________________________________________|\n_____________191_____________________________________________________________________________155\n |
146 -- 152 |
102 -- 108 |
-35.2093 kcal/mol |
10.3676 kcal/mol |
14.8184 kcal/mol |
121 156@LB@ | |@LB@5'-UAA...UCAUC A GUUAGCUAAUU AU U AA A C C UU CACUA...UGG-3'@LB@ CUCUU CAU CAG ACG UUGACA UGUUU AGCA GUU UG UGUU CUUUUUCAUC@LB@ ||||| ||| ||| ||: |||||| |||:: |||| ||| || |||: ||||:|||||@LB@ GAGAA GUA GUC UGU AACUGU ACAGG UCGU CAA AC ACAG GAAAGAGUAG@LB@3'-AUU...AAAAU GUU C A CG U GGUAUAUU A U AAGUU...CAA-5'@LB@ | |@LB@ 191 155@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -35.2093 kcal/mol@LB@Unfolding Energy - mRNA 10.3676 kcal/mol@LB@Unfolding Energy - sRNA 14.8184 kcal/mol@LB@@LB@Position - mRNA 122 -- 155@LB@Position - sRNA 156 -- 190@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 102 -- 108@LB@ |
