| 84 |
0.003073 |
0.1418 |
bsu31450 |
kinB |
-10.05 |
0.307083 |
220 -- 261 |
31 -- 74 |
sporulation kinase B |
|
_____________219___________________262\n_____________|_____________________|\n5'-AUG...CUUUA______________CU_____ACCAA...UGC-3'\n______________UUUUGUUUCCUAUU__UCUUU\n______________|:||:||:||:|||__|||||\n______________AGAAUAAGGGGUAA__AGAAA\n3'-AUU...GGUAG_____________________CUUUA...CAA-5'\n_____________|_____________________|\n_____________75____________________30\n |
248 -- 254 |
36 -- 42 |
-15.4229 kcal/mol |
1.85906 kcal/mol |
5.65941 kcal/mol |
219 262@LB@ | |@LB@5'-AUG...CUUUA CU ACCAA...UGC-3'@LB@ UUUUGUUUCCUAUU UCUUU@LB@ |:||:||:||:||| |||||@LB@ AGAAUAAGGGGUAA AGAAA@LB@3'-AUU...GGUAG CUUUA...CAA-5'@LB@ | |@LB@ 75 30@LB@@LB@@LB@Energy -10.05 kcal/mol@LB@Hybridization Energy -15.4229 kcal/mol@LB@Unfolding Energy - mRNA 1.85906 kcal/mol@LB@Unfolding Energy - sRNA 5.65941 kcal/mol@LB@@LB@Position - mRNA 220 -- 261@LB@Position - sRNA 31 -- 74@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 129 |
0.005948 |
0.1741 |
bsu13580 |
ykrV |
-12.37 |
0.114104 |
93 -- 132 |
31 -- 61 |
transaminase MtnE |
|
_____________92________________________________________133\n_____________|_________________________________________|\n5'-CAG...AAGAU________AUUU________UAACA_____AAUU_______ACUUU...ACG-3'\n______________AUCGU_CC____CAUCUUUU_____CCCCA____UUUCUUU\n______________|||||_||____|||||:||_____|||||____|||||||\n______________UAGCA_GG____GUAGAGAA_____GGGGU____AAAGAAA\n3'-AUU...UUUGU_____U______________UAA__________________CUUUA...CAA-5'\n_____________|_________________________________________|\n_____________62________________________________________30\n |
126 -- 132 |
31 -- 37 |
-24.5638 kcal/mol |
3.82436 kcal/mol |
10.4555 kcal/mol |
92 133@LB@ | |@LB@5'-CAG...AAGAU AUUU UAACA AAUU ACUUU...ACG-3'@LB@ AUCGU CC CAUCUUUU CCCCA UUUCUUU@LB@ ||||| || |||||:|| ||||| |||||||@LB@ UAGCA GG GUAGAGAA GGGGU AAAGAAA@LB@3'-AUU...UUUGU U UAA CUUUA...CAA-5'@LB@ | |@LB@ 62 30@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -24.5638 kcal/mol@LB@Unfolding Energy - mRNA 3.82436 kcal/mol@LB@Unfolding Energy - sRNA 10.4555 kcal/mol@LB@@LB@Position - mRNA 93 -- 132@LB@Position - sRNA 31 -- 61@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 132 |
0.006133 |
0.1741 |
bsu24800 |
yqgW |
-15.55 |
0.025469 |
1 -- 63 |
31 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
______________1_________________________________________________________________64\n______________|_________________________________________________________________|\n___________5'-__G____C_____________CCUACU____U____C________ACUA____CA___C_______UUUAU...CCG-3'\n______________GC_UUCG_UUG_AAUC__GGC______GGCA_UCGU_UCCAUUUC____UUAU__CCU_UUUCUUU\n______________||_||||_|||_||||__|||______::||_||||_:||||:||____||||__||:_|||||||\n______________CG_AAGC_AAC_UUAG__CCG______UUGU_AGCA_GGGUAGAG____AAUA__GGG_AAAGAAA\n3'-AUU...CUUAC___________G____AA___U_________U____U________________AG___U_______CUUUA...CAA-5'\n_____________|__________________________________________________________________|\n_____________86_________________________________________________________________30\n |
36 -- 42 |
48 -- 54 |
-38.6272 kcal/mol |
10.4973 kcal/mol |
14.833 kcal/mol |
1 64@LB@ | |@LB@ 5'- G C CCUACU U C ACUA CA C UUUAU...CCG-3'@LB@ GC UUCG UUG AAUC GGC GGCA UCGU UCCAUUUC UUAU CCU UUUCUUU@LB@ || |||| ||| |||| ||| ::|| |||| :||||:|| |||| ||: |||||||@LB@ CG AAGC AAC UUAG CCG UUGU AGCA GGGUAGAG AAUA GGG AAAGAAA@LB@3'-AUU...CUUAC G AA U U U AG U CUUUA...CAA-5'@LB@ | |@LB@ 86 30@LB@@LB@@LB@Energy -15.55 kcal/mol@LB@Hybridization Energy -38.6272 kcal/mol@LB@Unfolding Energy - mRNA 10.4973 kcal/mol@LB@Unfolding Energy - sRNA 14.833 kcal/mol@LB@@LB@Position - mRNA 1 -- 63@LB@Position - sRNA 31 -- 85@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 4 |
0.0001192 |
0.08823 |
bsu12150 |
yjgB |
-21.60 |
0.001260 |
37 -- 72 |
32 -- 79 |
Evidence 5: No homology to any previously reported sequences; PubMedId: 1580552 |
|
_____________36_______________________________________________73\n_____________|________________________________________________|\n5'-CCU...UGUGA____UU_____C____________________C_______________GCUUU...CAG-3'\n______________UUGU__UCUUG_CAG__________CCAUCUC__AUUCCCC_UUUCUU\n______________|||:__|||||_||:__________|||||||__|||||||_||||||\n______________AACG__AGAAC_GUU__________GGUAGAG__UAAGGGG_AAAGAA\n3'-AUU...GAAGC____UU_____C___UGUUAGCAUG_______AA_______U______ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________80_______________________________________________31\n |
60 -- 66 |
39 -- 45 |
-34.5565 kcal/mol |
2.03428 kcal/mol |
13.9015 kcal/mol |
36 73@LB@ | |@LB@5'-CCU...UGUGA UU C C GCUUU...CAG-3'@LB@ UUGU UCUUG CAG CCAUCUC AUUCCCC UUUCUU@LB@ |||: ||||| ||: ||||||| ||||||| ||||||@LB@ AACG AGAAC GUU GGUAGAG UAAGGGG AAAGAA@LB@3'-AUU...GAAGC UU C UGUUAGCAUG AA U ACUUU...CAA-5'@LB@ | |@LB@ 80 31@LB@@LB@@LB@Energy -21.60 kcal/mol@LB@Hybridization Energy -34.5565 kcal/mol@LB@Unfolding Energy - mRNA 2.03428 kcal/mol@LB@Unfolding Energy - sRNA 13.9015 kcal/mol@LB@@LB@Position - mRNA 37 -- 72@LB@Position - sRNA 32 -- 79@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 39 -- 45@LB@ |
| 7 |
0.0001858 |
0.08823 |
bsu34210 |
yvfG |
-17.07 |
0.012126 |
85 -- 142 |
32 -- 86 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________84____________________________________________________________143\n_____________|_____________________________________________________________|\n5'-GAG...GUUGA______AAUAC___________C____GUU__A__________GCUCAC____________CAUUG...ACU-3'\n______________GGCUUU_____UUGUA__CUUG_UGAA___UC_UAUCCAUCUU______UCCCC_UUUCUU\n______________|||||:_____|||:|__||||_::||___||_||:||||||:______|||||_||||||\n______________CCGAAG_____AACGU__GAAC_GUUU___AG_AUGGGUAGAG______AGGGG_AAAGAA\n3'-AUU...ACUUA______C_________UA____C____GUU__C__________AAUA_______U______ACUUU...CAA-5'\n_____________|_____________________________________________________________|\n_____________87____________________________________________________________31\n |
119 -- 125 |
48 -- 54 |
-40.7387 kcal/mol |
11.2598 kcal/mol |
14.8836 kcal/mol |
84 143@LB@ | |@LB@5'-GAG...GUUGA AAUAC C GUU A GCUCAC CAUUG...ACU-3'@LB@ GGCUUU UUGUA CUUG UGAA UC UAUCCAUCUU UCCCC UUUCUU@LB@ |||||: |||:| |||| ::|| || ||:||||||: ||||| ||||||@LB@ CCGAAG AACGU GAAC GUUU AG AUGGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACUUA C UA C GUU C AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 87 31@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -40.7387 kcal/mol@LB@Unfolding Energy - mRNA 11.2598 kcal/mol@LB@Unfolding Energy - sRNA 14.8836 kcal/mol@LB@@LB@Position - mRNA 85 -- 142@LB@Position - sRNA 32 -- 86@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 12 |
0.0004273 |
0.1011 |
bsu13640 |
spo0E |
-21.66 |
0.001221 |
136 -- 180 |
32 -- 78 |
aspartyl-phosphate phosphatase Spo0E |
|
_____________135________________________________________________181\n_____________|__________________________________________________|\n5'-GGC...UAAUC______AAU__________A______A___AAA_________________AGCGG...AUG-3'\n______________UGUAAU____GC__ACAAU___ACCC_AUC___CUUGUUUCCUAUUUCUU\n______________||:|||____||__|||||___||||_|||___|||:||:||:|||||||\n______________ACGUUA____CG__UGUUA___UGGG_UAG___GAAUAAGGGGUAAAGAA\n3'-AUU...AAGCA______GAAC__UU_____GCA________A___________________ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________79_________________________________________________31\n |
172 -- 178 |
34 -- 40 |
-36.2602 kcal/mol |
3.78665 kcal/mol |
13.8985 kcal/mol |
135 181@LB@ | |@LB@5'-GGC...UAAUC AAU A A AAA AGCGG...AUG-3'@LB@ UGUAAU GC ACAAU ACCC AUC CUUGUUUCCUAUUUCUU@LB@ ||:||| || ||||| |||| ||| |||:||:||:|||||||@LB@ ACGUUA CG UGUUA UGGG UAG GAAUAAGGGGUAAAGAA@LB@3'-AUU...AAGCA GAAC UU GCA A ACUUU...CAA-5'@LB@ | |@LB@ 79 31@LB@@LB@@LB@Energy -21.66 kcal/mol@LB@Hybridization Energy -36.2602 kcal/mol@LB@Unfolding Energy - mRNA 3.78665 kcal/mol@LB@Unfolding Energy - sRNA 13.8985 kcal/mol@LB@@LB@Position - mRNA 136 -- 180@LB@Position - sRNA 32 -- 78@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 64 |
0.002007 |
0.1215 |
bsu37680 |
ywfH |
-19.51 |
0.003623 |
4 -- 58 |
32 -- 83 |
bacilysin biosynthesis oxidoreductase YwfH |
|
_____________3___________________________________________________________59\n_____________|___________________________________________________________|\n_________5'-AA___G______U____U__C_____________U__G____AUACAC_______A_____AUAUG...AUU-3'\n______________UUU_GUU_CA_UCUU_GU_GGCAAUC__ACCU_UC_CUUG______UUCCUCA_UUCUU\n______________:||_|||_||_||||_|:_::|||||__|||:_||_|||:______||||:||_|||||\n______________GAA_CAA_GU_AGAA_CG_UUGUUAG__UGGG_AG_GAAU______AAGGGGU_AAGAA\n3'-AUU...UUACC___G___C__U____C__U_______CA____U__A_________________A_____ACUUU...CAA-5'\n_____________|___________________________________________________________|\n_____________84__________________________________________________________31\n |
46 -- 52 |
38 -- 44 |
-36.3278 kcal/mol |
5.00359 kcal/mol |
14.596 kcal/mol |
3 59@LB@ | |@LB@ 5'-AA G U U C U G AUACAC A AUAUG...AUU-3'@LB@ UUU GUU CA UCUU GU GGCAAUC ACCU UC CUUG UUCCUCA UUCUU@LB@ :|| ||| || |||| |: ::||||| |||: || |||: ||||:|| |||||@LB@ GAA CAA GU AGAA CG UUGUUAG UGGG AG GAAU AAGGGGU AAGAA@LB@3'-AUU...UUACC G C U C U CA U A A ACUUU...CAA-5'@LB@ | |@LB@ 84 31@LB@@LB@@LB@Energy -19.51 kcal/mol@LB@Hybridization Energy -36.3278 kcal/mol@LB@Unfolding Energy - mRNA 5.00359 kcal/mol@LB@Unfolding Energy - sRNA 14.596 kcal/mol@LB@@LB@Position - mRNA 4 -- 58@LB@Position - sRNA 32 -- 83@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 78 |
0.002646 |
0.1315 |
bsu37410 |
albE |
-16.69 |
0.014616 |
33 -- 81 |
32 -- 77 |
antilisterial bacteriocin subtilosin biosynthesis protein AlbE |
|
_____________32_________________________________________________82\n_____________|__________________________________________________|\n5'-CUU...AUGGA_______CU_____AU____GU_______G__UUGUC______UA_____AGCCA...AAA-3'\n______________GCGGUUU___CAAA__AUCG__CUUAUCU_UU_____UUCCCC__UUCUU\n______________||::|:|___||||__||||__|::||||_||_____||||||__|||||\n______________CGUUAGA___GUUU__UAGC__GGGUAGA_AA_____AAGGGG__AAGAA\n3'-AUU...AGCAA_______ACC____GU____AU_______G__U__________UA_____ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________78_________________________________________________31\n |
54 -- 60 |
49 -- 55 |
-34.8992 kcal/mol |
7.30498 kcal/mol |
13.8943 kcal/mol |
32 82@LB@ | |@LB@5'-CUU...AUGGA CU AU GU G UUGUC UA AGCCA...AAA-3'@LB@ GCGGUUU CAAA AUCG CUUAUCU UU UUCCCC UUCUU@LB@ ||::|:| |||| |||| |::|||| || |||||| |||||@LB@ CGUUAGA GUUU UAGC GGGUAGA AA AAGGGG AAGAA@LB@3'-AUU...AGCAA ACC GU AU G U UA ACUUU...CAA-5'@LB@ | |@LB@ 78 31@LB@@LB@@LB@Energy -16.69 kcal/mol@LB@Hybridization Energy -34.8992 kcal/mol@LB@Unfolding Energy - mRNA 7.30498 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 33 -- 81@LB@Position - sRNA 32 -- 77@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 106 |
0.004415 |
0.1614 |
bsu10790 |
asnO |
-17.17 |
0.011578 |
3 -- 52 |
32 -- 73 |
asparagine synthetase 3 |
|
_____________2__________________________________________________53\n_____________|__________________________________________________|\n_________5'-CC___GUAUAU_________U________AUCCAUGCUG_____UU______CAUUC...CUG-3'\n______________UCU______AACAAUUGU_UCCGUUUC__________UCUCC__UUUCUU\n______________|||______||||||:||_:||:|:||__________||:||__||||||\n______________AGA______UUGUUAGCA_GGGUAGAG__________AGGGG__AAAGAA\n3'-AUU...ACGUU___ACCGU__________U________AAUA___________U_______ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________74_________________________________________________31\n |
23 -- 29 |
48 -- 54 |
-28.5385 kcal/mol |
1.12136 kcal/mol |
12.9474 kcal/mol |
2 53@LB@ | |@LB@ 5'-CC GUAUAU U AUCCAUGCUG UU CAUUC...CUG-3'@LB@ UCU AACAAUUGU UCCGUUUC UCUCC UUUCUU@LB@ ||| ||||||:|| :||:|:|| ||:|| ||||||@LB@ AGA UUGUUAGCA GGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACGUU ACCGU U AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 74 31@LB@@LB@@LB@Energy -17.17 kcal/mol@LB@Hybridization Energy -28.5385 kcal/mol@LB@Unfolding Energy - mRNA 1.12136 kcal/mol@LB@Unfolding Energy - sRNA 12.9474 kcal/mol@LB@@LB@Position - mRNA 3 -- 52@LB@Position - sRNA 32 -- 73@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 115 |
0.005245 |
0.1741 |
bsu07690 |
yflG |
-14.30 |
0.046489 |
29 -- 56 |
32 -- 57 |
methionine aminopeptidase 2 |
|
_____________28___________________________57\n_____________|____________________________|\n5'-UUC...AAGCA________CCC__C__A____U______CAAAU...GCA-3'\n______________UACCCAUC___CU_AU_CCCU_UUUUUU\n______________||||||||___||_||_|||:_|||:||\n______________AUGGGUAG___GA_UA_GGGG_AAAGAA\n3'-AUU...UUAGC________A____A__A____U______ACUUU...CAA-5'\n_____________|____________________________|\n_____________58___________________________31\n |
30 -- 36 |
50 -- 56 |
-21.2542 kcal/mol |
0.575621 kcal/mol |
9.1585 kcal/mol |
28 57@LB@ | |@LB@5'-UUC...AAGCA CCC C A U CAAAU...GCA-3'@LB@ UACCCAUC CU AU CCCU UUUUUU@LB@ |||||||| || || |||: |||:||@LB@ AUGGGUAG GA UA GGGG AAAGAA@LB@3'-AUU...UUAGC A A A U ACUUU...CAA-5'@LB@ | |@LB@ 58 31@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -21.2542 kcal/mol@LB@Unfolding Energy - mRNA 0.575621 kcal/mol@LB@Unfolding Energy - sRNA 9.1585 kcal/mol@LB@@LB@Position - mRNA 29 -- 56@LB@Position - sRNA 32 -- 57@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 50 -- 56@LB@ |
