| 74 |
0.002384 |
0.1242 |
bsu09450 |
yhdF |
-14.81 |
0.036487 |
208 -- 244 |
132 -- 159 |
oxidoreductase |
|
_____________207____________________________________245\n_____________|______________________________________|\n5'-UCC...GAACC__________GACAAACAGAAG____AGAA________UCAGG...AGG-3'\n______________CAAU_GGACA____________GACA____CCGCAGCA\n______________|||:_|||||____________||||____||||||||\n______________GUUG_CCUGU____________CUGU____GGCGUCGU\n3'-AUU...AAGUA____U_____AA______________AACA________UCAAG...CAA-5'\n_____________|______________________________________|\n_____________160____________________________________131\n |
238 -- 244 |
132 -- 138 |
-26.7883 kcal/mol |
3.14586 kcal/mol |
9.22644 kcal/mol |
207 245@LB@ | |@LB@5'-UCC...GAACC GACAAACAGAAG AGAA UCAGG...AGG-3'@LB@ CAAU GGACA GACA CCGCAGCA@LB@ |||: ||||| |||| ||||||||@LB@ GUUG CCUGU CUGU GGCGUCGU@LB@3'-AUU...AAGUA U AA AACA UCAAG...CAA-5'@LB@ | |@LB@ 160 131@LB@@LB@@LB@Energy -14.81 kcal/mol@LB@Hybridization Energy -26.7883 kcal/mol@LB@Unfolding Energy - mRNA 3.14586 kcal/mol@LB@Unfolding Energy - sRNA 9.22644 kcal/mol@LB@@LB@Position - mRNA 208 -- 244@LB@Position - sRNA 132 -- 159@LB@Position Seed - mRNA 238 -- 244@LB@Position Seed - sRNA 132 -- 138@LB@ |
| 149 |
0.006788 |
0.1741 |
bsu28960 |
ytxC |
-13.61 |
0.064351 |
236 -- 300 |
132 -- 186 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________235________________________301\n_____________|__________________________|\n________5'-CGA__U________U______________CUUUC...GCA-3'\n______________GC__UUUUGGC_GGCAAUC_UGCCCG\n______________||__|:|||||_::|||||_|:|||:\n______________CG__AGAACCG_UUGUUAG_AUGGGU\n3'-AUU...AGCAA__UU_______U_______C______AGAGA...CAA-5'\n_____________|__________________________|\n_____________187________________________131\n |
7 -- 13 |
67 -- 73 |
-27.0931 kcal/mol |
7.22978 kcal/mol |
8.4393 kcal/mol |
235 301@LB@ | |@LB@ 5'-CGA U U CUUUC...GCA-3'@LB@ GC UUUUGGC GGCAAUC UGCCCG@LB@ || |:||||| ::||||| |:|||:@LB@ CG AGAACCG UUGUUAG AUGGGU@LB@3'-AUU...AGCAA UU U C AGAGA...CAA-5'@LB@ | |@LB@ 187 131@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -27.0931 kcal/mol@LB@Unfolding Energy - mRNA 7.22978 kcal/mol@LB@Unfolding Energy - sRNA 8.4393 kcal/mol@LB@@LB@Position - mRNA 236 -- 300@LB@Position - sRNA 132 -- 186@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 39 |
0.001272 |
0.1206 |
bsu38090 |
vpr |
-13.65 |
0.063330 |
246 -- 286 |
133 -- 174 |
minor extracellular protease vpr |
|
_____________245__________________________________________287\n_____________|____________________________________________|\n5'-AUU...CUUAU_______G_______C__________ACG______CAG______UCCGG...CAA-3'\n______________UUUUUGC___UUAUC_ACAGG_CAUU___GGCGUU____GCAGC\n______________|||||:|___|:|||_|||||_||||___|:|:||____|||||\n______________AAAAAUG___AGUAG_UGUCC_GUAA___CUGUAA____CGUCG\n3'-AUU...CGAAA_______AGA_____U_____U_____________CAGG_____UUCAA...CAA-5'\n_____________|____________________________________________|\n_____________175__________________________________________132\n |
247 -- 253 |
167 -- 173 |
-30.59 kcal/mol |
9.01512 kcal/mol |
10.7895 kcal/mol |
245 287@LB@ | |@LB@5'-AUU...CUUAU G C ACG CAG UCCGG...CAA-3'@LB@ UUUUUGC UUAUC ACAGG CAUU GGCGUU GCAGC@LB@ |||||:| |:||| ||||| |||| |:|:|| |||||@LB@ AAAAAUG AGUAG UGUCC GUAA CUGUAA CGUCG@LB@3'-AUU...CGAAA AGA U U CAGG UUCAA...CAA-5'@LB@ | |@LB@ 175 132@LB@@LB@@LB@Energy -13.65 kcal/mol@LB@Hybridization Energy -30.59 kcal/mol@LB@Unfolding Energy - mRNA 9.01512 kcal/mol@LB@Unfolding Energy - sRNA 10.7895 kcal/mol@LB@@LB@Position - mRNA 246 -- 286@LB@Position - sRNA 133 -- 174@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 167 -- 173@LB@ |
| 65 |
0.002089 |
0.123 |
bsu15330 |
sigG |
-7.32 |
0.735765 |
1 -- 8 |
133 -- 140 |
RNA polymerase sigma-G factor |
|
______________1_______9\n______________|_______|\n___________5'-________UUGAG...AUG-3'\n______________UUCGCGGC\n______________|:|||:||\n______________AGGCGUCG\n3'-AUU...GUAAC________UUCAA...CAA-5'\n_____________|________|\n_____________141______132\n |
2 -- 8 |
133 -- 139 |
-12.0754 kcal/mol |
1.63369 kcal/mol |
4.3229 kcal/mol |
1 9@LB@ | |@LB@ 5'- UUGAG...AUG-3'@LB@ UUCGCGGC@LB@ |:|||:||@LB@ AGGCGUCG@LB@3'-AUU...GUAAC UUCAA...CAA-5'@LB@ | |@LB@ 141 132@LB@@LB@@LB@Energy -7.32 kcal/mol@LB@Hybridization Energy -12.0754 kcal/mol@LB@Unfolding Energy - mRNA 1.63369 kcal/mol@LB@Unfolding Energy - sRNA 4.3229 kcal/mol@LB@@LB@Position - mRNA 1 -- 8@LB@Position - sRNA 133 -- 140@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 133 -- 139@LB@ |
| 200 |
0.009037 |
0.1751 |
bsu15760 |
prpC |
-17.37 |
0.010506 |
124 -- 148 |
133 -- 158 |
protein phosphatase PrpC |
|
_____________123_________________________149\n_____________|___________________________|\n5'-ACG...AAGAG___A_______________A_______CUGCG...UUG-3'\n______________AGC_AGGACA_UGACAUUG__CGCAGC\n______________|:|_||||||_||||||||__||||||\n______________UUG_UCCUGU_ACUGUAAC__GCGUCG\n3'-AUU...AGUAG__________A________AG______UUCAA...CAA-5'\n_____________|___________________________|\n_____________159_________________________132\n |
135 -- 141 |
141 -- 147 |
-32.4946 kcal/mol |
7.19227 kcal/mol |
8.48433 kcal/mol |
123 149@LB@ | |@LB@5'-ACG...AAGAG A A CUGCG...UUG-3'@LB@ AGC AGGACA UGACAUUG CGCAGC@LB@ |:| |||||| |||||||| ||||||@LB@ UUG UCCUGU ACUGUAAC GCGUCG@LB@3'-AUU...AGUAG A AG UUCAA...CAA-5'@LB@ | |@LB@ 159 132@LB@@LB@@LB@Energy -17.37 kcal/mol@LB@Hybridization Energy -32.4946 kcal/mol@LB@Unfolding Energy - mRNA 7.19227 kcal/mol@LB@Unfolding Energy - sRNA 8.48433 kcal/mol@LB@@LB@Position - mRNA 124 -- 148@LB@Position - sRNA 133 -- 158@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 141 -- 147@LB@ |
| 26 |
0.0007049 |
0.1051 |
bsu24750 |
yqhB |
-20.67 |
0.002015 |
266 -- 294 |
134 -- 164 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pm: membrane component' |
|
_____________265_____________________________295\n_____________|_______________________________|\n5'-UUC...CUCAU______________UUUU_____________AAUUUU-3'\n______________UUUGUUAACAGGAU_____CGUUG_CCGUAG\n______________||::|:|||||||:_____|:|||_|||:||\n______________AAGUAGUUGUCCUG_____GUAAC_GGCGUC\n3'-AUU...AUGAG______________UAACU_____A______GUUCA...CAA-5'\n_____________|_______________________________|\n_____________165_____________________________133\n |
273 -- 279 |
151 -- 157 |
-31.5872 kcal/mol |
2.9023 kcal/mol |
8.693 kcal/mol |
265 295@LB@ | |@LB@5'-UUC...CUCAU UUUU AAUUUU-3'@LB@ UUUGUUAACAGGAU CGUUG CCGUAG@LB@ ||::|:|||||||: |:||| |||:||@LB@ AAGUAGUUGUCCUG GUAAC GGCGUC@LB@3'-AUU...AUGAG UAACU A GUUCA...CAA-5'@LB@ | |@LB@ 165 133@LB@@LB@@LB@Energy -20.67 kcal/mol@LB@Hybridization Energy -31.5872 kcal/mol@LB@Unfolding Energy - mRNA 2.9023 kcal/mol@LB@Unfolding Energy - sRNA 8.693 kcal/mol@LB@@LB@Position - mRNA 266 -- 294@LB@Position - sRNA 134 -- 164@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 151 -- 157@LB@ |
| 172 |
0.008071 |
0.1751 |
bsu03860 |
ycnD |
-16.76 |
0.014170 |
153 -- 167 |
135 -- 149 |
FMN reductase |
|
_____________152_____________168\n_____________|_______________|\n5'-CGC...UCCUC_______________CAGAG...CGG-3'\n______________UUGAUGUUGUCCGCA\n______________||||::|||||||||\n______________AACUGUAACAGGCGU\n3'-AUU...CCUGU_______________CGUUC...CAA-5'\n_____________|_______________|\n_____________150_____________134\n |
161 -- 167 |
135 -- 141 |
-23.0656 kcal/mol |
2.48067 kcal/mol |
5.52377 kcal/mol |
152 168@LB@ | |@LB@5'-CGC...UCCUC CAGAG...CGG-3'@LB@ UUGAUGUUGUCCGCA@LB@ ||||::|||||||||@LB@ AACUGUAACAGGCGU@LB@3'-AUU...CCUGU CGUUC...CAA-5'@LB@ | |@LB@ 150 134@LB@@LB@@LB@Energy -16.76 kcal/mol@LB@Hybridization Energy -23.0656 kcal/mol@LB@Unfolding Energy - mRNA 2.48067 kcal/mol@LB@Unfolding Energy - sRNA 5.52377 kcal/mol@LB@@LB@Position - mRNA 153 -- 167@LB@Position - sRNA 135 -- 149@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 135 -- 141@LB@ |
| 10 |
0.0002277 |
0.08823 |
bsu05690 |
ydhB |
-21.23 |
0.001523 |
113 -- 150 |
136 -- 182 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
bsu12400 |
_____________112_______________________________151\n_____________|_________________________________|\n5'-CAU...UGUUU__________________C______ACC_____CAGAA...UCC-3'\n______________UUCUUCGUCAAUAGGACA____CAU___UCCGC\n______________:|||||:||||:||||||____|||___|||||\n______________GAGAAGUAGUUGUCCUGU____GUA___AGGCG\n3'-AUU...AAAAU__________________AACU___AC______UCGUU...CAA-5'\n_____________|_________________________________|\n_____________183_______________________________135\n |
132 -- 138 |
150 -- 156 |
-33.3473 kcal/mol |
6.29637 kcal/mol |
7.50175 kcal/mol |
112 151@LB@ | |@LB@5'-CAU...UGUUU C ACC CAGAA...UCC-3'@LB@ UUCUUCGUCAAUAGGACA CAU UCCGC@LB@ :|||||:||||:|||||| ||| |||||@LB@ GAGAAGUAGUUGUCCUGU GUA AGGCG@LB@3'-AUU...AAAAU AACU AC UCGUU...CAA-5'@LB@ | |@LB@ 183 135@LB@@LB@@LB@Energy -21.23 kcal/mol@LB@Hybridization Energy -33.3473 kcal/mol@LB@Unfolding Energy - mRNA 6.29637 kcal/mol@LB@Unfolding Energy - sRNA 7.50175 kcal/mol@LB@@LB@Position - mRNA 113 -- 150@LB@Position - sRNA 136 -- 182@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 150 -- 156@LB@ |
| 176 |
0.008122 |
0.1751 |
bsu16520 |
frr |
-16.32 |
0.017549 |
85 -- 113 |
136 -- 167 |
ribosome-recycling factor |
|
_____________84________________________________114\n_____________|_________________________________|\n5'-AGA...GCUUC_____________U_____A_____________UUAUC...AUC-3'\n______________CUCUUUAU__GCA_GGACA_UGACAU__UCCGC\n______________|||||:||__:||_|||||_||||||__|||||\n______________GAGAAGUA__UGU_CCUGU_ACUGUA__AGGCG\n3'-AUU...AAAAU________GU_________A______AC_____UCGUU...CAA-5'\n_____________|_________________________________|\n_____________168_______________________________135\n |
86 -- 92 |
160 -- 166 |
-32.0013 kcal/mol |
8.50527 kcal/mol |
7.50175 kcal/mol |
84 114@LB@ | |@LB@5'-AGA...GCUUC U A UUAUC...AUC-3'@LB@ CUCUUUAU GCA GGACA UGACAU UCCGC@LB@ |||||:|| :|| ||||| |||||| |||||@LB@ GAGAAGUA UGU CCUGU ACUGUA AGGCG@LB@3'-AUU...AAAAU GU A AC UCGUU...CAA-5'@LB@ | |@LB@ 168 135@LB@@LB@@LB@Energy -16.32 kcal/mol@LB@Hybridization Energy -32.0013 kcal/mol@LB@Unfolding Energy - mRNA 8.50527 kcal/mol@LB@Unfolding Energy - sRNA 7.50175 kcal/mol@LB@@LB@Position - mRNA 85 -- 113@LB@Position - sRNA 136 -- 167@LB@Position Seed - mRNA 86 -- 92@LB@Position Seed - sRNA 160 -- 166@LB@ |
| 147 |
0.006774 |
0.1741 |
bsu08550 |
sspK |
-14.99 |
0.033457 |
165 -- 208 |
137 -- 183 |
small acid-soluble spore protein K |
|
_____________164_____________________________209\n_____________|_______________________________|\n5'-UUU...UUGAU___GAACC___G_____________G_____AAAUA...ACG-3'\n______________CUC_____UAG_GGGAUAU_GACAU_GUCCG\n______________|||_____:|:_:|||:||_|||||_|||||\n______________GAG_____GUU_UCCUGUA_CUGUA_CAGGC\n3'-AUU...AAAAU___AAGUA___G_______A_____A_____GUCGU...CAA-5'\n_____________|_______________________________|\n_____________184_____________________________136\n |
191 -- 197 |
149 -- 155 |
-28.8408 kcal/mol |
8.70873 kcal/mol |
7.49334 kcal/mol |
164 209@LB@ | |@LB@5'-UUU...UUGAU GAACC G G AAAUA...ACG-3'@LB@ CUC UAG GGGAUAU GACAU GUCCG@LB@ ||| :|: :|||:|| ||||| |||||@LB@ GAG GUU UCCUGUA CUGUA CAGGC@LB@3'-AUU...AAAAU AAGUA G A A GUCGU...CAA-5'@LB@ | |@LB@ 184 136@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -28.8408 kcal/mol@LB@Unfolding Energy - mRNA 8.70873 kcal/mol@LB@Unfolding Energy - sRNA 7.49334 kcal/mol@LB@@LB@Position - mRNA 165 -- 208@LB@Position - sRNA 137 -- 183@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 149 -- 155@LB@ |
