Input and runtime details for job 1654565 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1654565 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:23:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:05 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:25:53 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:26:01 CET 2018
	Job Completed		@ Fri Feb 16 22:26:34 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1654565&toolName=CopraRNA ( 30 days expiry )

Description of the job

FnrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
88	0.002437	0.09689	b0940	elfC	-8.14	0.278491	92 -- 131	23 -- 58	putative outer membrane fimbrial subunit export usher protein	b3144 b0532 b4317	_____________91_________________________________________132\n_____________\|__________________________________________\|\n5'-AUU...CUGCC________CCAAA_____GC___________AUCGAA_____AAUGA...CAC-3'\n______________AAAGGGGG______AAUA___AGCGUAACGU______CCAUC\n______________\|\|\|\|\|::\|______\|\|\|\|___\|\|\|\|:\|\|\|\|:______\|\|\|\|\|\n______________UUUCCUUC______UUAU___UCGCGUUGCG______GGUAG\n3'-UUU...AGUUU________AUUCUG____ACC_____________________CGAAC...ACG-5'\n_____________\|__________________________________________\|\n_____________59_________________________________________22\n	112 -- 118	30 -- 36	-29.3003 kcal/mol	7.72593 kcal/mol	13.4354 kcal/mol	91 132@LB@ \| \|@LB@5'-AUU...CUGCC CCAAA GC AUCGAA AAUGA...CAC-3'@LB@ AAAGGGGG AAUA AGCGUAACGU CCAUC@LB@ \|\|\|\|\|::\| \|\|\|\| \|\|\|\|:\|\|\|\|: \|\|\|\|\|@LB@ UUUCCUUC UUAU UCGCGUUGCG GGUAG@LB@3'-UUU...AGUUU AUUCUG ACC CGAAC...ACG-5'@LB@ \| \|@LB@ 59 22@LB@@LB@@LB@Energy -8.14 kcal/mol@LB@Hybridization Energy -29.3003 kcal/mol@LB@Unfolding Energy - mRNA 7.72593 kcal/mol@LB@Unfolding Energy - sRNA 13.4354 kcal/mol@LB@@LB@Position - mRNA 92 -- 131@LB@Position - sRNA 23 -- 58@LB@Position Seed - mRNA 112 -- 118@LB@Position Seed - sRNA 30 -- 36@LB@
112	0.003634	0.1131	b1473	yddG	-8.08	0.286569	177 -- 226	24 -- 55	aromatic amino acid exporter		_____________176________________________________________________227\n_____________\|__________________________________________________\|\n5'-GCG...UUAAC__C___A_______AACA_C____AUGACACGACAAAAA___________AGGGC...CGG-3'\n______________GG_AAG_GAGACAA____G_GAGC_______________GCAACGCUCAU\n______________\|\|_\|\|\|_:\|\|\|\|\|\|____\|_\|\|\|\|_______________\|\|\|\|\|\|\|:\|\|\|\n______________CC_UUC_UUCUGUU____C_CUCG_______________CGUUGCGGGUA\n3'-UUU...UUUUU______A_______AUA_________________________________GCGAA...ACG-5'\n_____________\|__________________________________________________\|\n_____________56_________________________________________________23\n	216 -- 222	28 -- 34	-30.4707 kcal/mol	9.38627 kcal/mol	13.0079 kcal/mol	176 227@LB@ \| \|@LB@5'-GCG...UUAAC C A AACA C AUGACACGACAAAAA AGGGC...CGG-3'@LB@ GG AAG GAGACAA G GAGC GCAACGCUCAU@LB@ \|\| \|\|\| :\|\|\|\|\|\| \| \|\|\|\| \|\|\|\|\|\|\|:\|\|\|@LB@ CC UUC UUCUGUU C CUCG CGUUGCGGGUA@LB@3'-UUU...UUUUU A AUA GCGAA...ACG-5'@LB@ \| \|@LB@ 56 23@LB@@LB@@LB@Energy -8.08 kcal/mol@LB@Hybridization Energy -30.4707 kcal/mol@LB@Unfolding Energy - mRNA 9.38627 kcal/mol@LB@Unfolding Energy - sRNA 13.0079 kcal/mol@LB@@LB@Position - mRNA 177 -- 226@LB@Position - sRNA 24 -- 55@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 28 -- 34@LB@
83	0.001966	0.08273	b1584	speG	-9.79	0.123144	169 -- 214	25 -- 77	spermidine N(1)-acetyltransferase		_____________168_____________________________________________________215\n_____________\|_______________________________________________________\|\n5'-UCU...UAACC__________________________U____G____CGUUAU___CA________CAGUG...GGU-3'\n______________UGUUAU____UGAUUUA____AGGAA_GUAA_GACA_______GC__AGCGCCCA\n______________\|\|:\|\|\|____\|:\|\|\|:\|____\|\|\|\|\|_\|\|\|\|_\|\|\|\|_______\|\|__\|:\|\|\|\|\|\|\n______________ACGAUA____AUUAAGU____UCCUU_CAUU_CUGU_______CG__UUGCGGGU\n3'-UUU...UUAAC______CGUC_______UUUU_______________UAUACCU__CG________AGCGA...ACG-5'\n_____________\|_______________________________________________________\|\n_____________78______________________________________________________24\n	208 -- 214	25 -- 31	-32.6914 kcal/mol	9.23779 kcal/mol	13.6641 kcal/mol	168 215@LB@ \| \|@LB@5'-UCU...UAACC U G CGUUAU CA CAGUG...GGU-3'@LB@ UGUUAU UGAUUUA AGGAA GUAA GACA GC AGCGCCCA@LB@ \|\|:\|\|\| \|:\|\|\|:\| \|\|\|\|\| \|\|\|\| \|\|\|\| \|\| \|:\|\|\|\|\|\|@LB@ ACGAUA AUUAAGU UCCUU CAUU CUGU CG UUGCGGGU@LB@3'-UUU...UUAAC CGUC UUUU UAUACCU CG AGCGA...ACG-5'@LB@ \| \|@LB@ 78 24@LB@@LB@@LB@Energy -9.79 kcal/mol@LB@Hybridization Energy -32.6914 kcal/mol@LB@Unfolding Energy - mRNA 9.23779 kcal/mol@LB@Unfolding Energy - sRNA 13.6641 kcal/mol@LB@@LB@Position - mRNA 169 -- 214@LB@Position - sRNA 25 -- 77@LB@Position Seed - mRNA 208 -- 214@LB@Position Seed - sRNA 25 -- 31@LB@
98	0.003129	0.1114	b0357	frmR	-9.19	0.168108	184 -- 207	25 -- 48	regulator protein that represses frmRAB operon		_____________183______________________208\n_____________\|________________________\|\n5'-CUG...CAUGC________A__U___AA_______GUACU...CCG-3'\n______________AGAUGAUG_GG_GCG__AUGCCCA\n______________\|\|\|::\|\|:_\|\|_\|\|\|__\|:\|\|\|\|\|\n______________UCUGUUAU_CC_CGC__UGCGGGU\n3'-UUU...UUCAU________A__U___GU_______AGCGA...ACG-5'\n_____________\|________________________\|\n_____________49_______________________24\n	201 -- 207	25 -- 31	-27.7971 kcal/mol	6.33291 kcal/mol	12.271 kcal/mol	183 208@LB@ \| \|@LB@5'-CUG...CAUGC A U AA GUACU...CCG-3'@LB@ AGAUGAUG GG GCG AUGCCCA@LB@ \|\|\|::\|\|: \|\| \|\|\| \|:\|\|\|\|\|@LB@ UCUGUUAU CC CGC UGCGGGU@LB@3'-UUU...UUCAU A U GU AGCGA...ACG-5'@LB@ \| \|@LB@ 49 24@LB@@LB@@LB@Energy -9.19 kcal/mol@LB@Hybridization Energy -27.7971 kcal/mol@LB@Unfolding Energy - mRNA 6.33291 kcal/mol@LB@Unfolding Energy - sRNA 12.271 kcal/mol@LB@@LB@Position - mRNA 184 -- 207@LB@Position - sRNA 25 -- 48@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 25 -- 31@LB@
86	0.002341	0.09591	b0144	gluQ	-7.21	0.411815	221 -- 264	26 -- 65	glutamyl-Q tRNA(Asp) synthetase		_____________220__________________________________________265\n_____________\|____________________________________________\|\n5'-AGA...UUUUU__________AU__C__ACAC_______AUU__C___UU_____CCUCU...UGA-3'\n______________GUUCAAAGAG__GA_AG____ACAGUAU___GG_CGC__CGCCC\n______________:\|\|\|\|\|\|:\|:__\|\|_\|\|____\|\|\|:\|\|\|___:\|_\|\|\|__\|\|\|\|\|\n______________UAAGUUUUUU__CU_UC____UGUUAUA___UC_GCG__GCGGG\n3'-UUU...GUCAU__________C______AUUC_______CC_______UU_____UAGCG...ACG-5'\n_____________\|____________________________________________\|\n_____________66___________________________________________25\n	242 -- 248	40 -- 46	-28.4973 kcal/mol	7.75098 kcal/mol	13.5387 kcal/mol	220 265@LB@ \| \|@LB@5'-AGA...UUUUU AU C ACAC AUU C UU CCUCU...UGA-3'@LB@ GUUCAAAGAG GA AG ACAGUAU GG CGC CGCCC@LB@ :\|\|\|\|\|\|:\|: \|\| \|\| \|\|\|:\|\|\| :\| \|\|\| \|\|\|\|\|@LB@ UAAGUUUUUU CU UC UGUUAUA UC GCG GCGGG@LB@3'-UUU...GUCAU C AUUC CC UU UAGCG...ACG-5'@LB@ \| \|@LB@ 66 25@LB@@LB@@LB@Energy -7.21 kcal/mol@LB@Hybridization Energy -28.4973 kcal/mol@LB@Unfolding Energy - mRNA 7.75098 kcal/mol@LB@Unfolding Energy - sRNA 13.5387 kcal/mol@LB@@LB@Position - mRNA 221 -- 264@LB@Position - sRNA 26 -- 65@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 40 -- 46@LB@
113	0.00365	0.1131	b3154	yhbP	-10.91	0.065979	249 -- 297	26 -- 85	UPF0306 family protein		_____________248___________________________________________________________298\n_____________\|_____________________________________________________________\|\n5'-AAA...AUGUU____C___GUG_______C_____________________ACUC____U____U_______UUU-3'\n______________GUCA_UUG___UGUGCAG___________AGGAAG_GGGA_____UGG_GCGC_AAUGCCU\n______________:\|\|\|_\|\|\|___\|:\|\|\|\|\|___________\|\|\|\|\|\|_::\|\|_____\|\|\|_\|\|\|\|_\|\|:\|\|\|:\n______________UAGU_AAC___AUACGUC___________UCCUUC_UUCU_____ACC_CGCG_UUGCGGG\n3'-UUU...AUGCU____U___ACG_______AUUAAGUUUUU______A____GUUAU___U____________UAGCG...ACG-5'\n_____________\|_____________________________________________________________\|\n_____________86____________________________________________________________25\n	260 -- 266	68 -- 74	-42.816 kcal/mol	18.2405 kcal/mol	13.6657 kcal/mol	248 298@LB@ \| \|@LB@5'-AAA...AUGUU C GUG C ACUC U U UUU-3'@LB@ GUCA UUG UGUGCAG AGGAAG GGGA UGG GCGC AAUGCCU@LB@ :\|\|\| \|\|\| \|:\|\|\|\|\| \|\|\|\|\|\| ::\|\| \|\|\| \|\|\|\| \|\|:\|\|\|:@LB@ UAGU AAC AUACGUC UCCUUC UUCU ACC CGCG UUGCGGG@LB@3'-UUU...AUGCU U ACG AUUAAGUUUUU A GUUAU U UAGCG...ACG-5'@LB@ \| \|@LB@ 86 25@LB@@LB@@LB@Energy -10.91 kcal/mol@LB@Hybridization Energy -42.816 kcal/mol@LB@Unfolding Energy - mRNA 18.2405 kcal/mol@LB@Unfolding Energy - sRNA 13.6657 kcal/mol@LB@@LB@Position - mRNA 249 -- 297@LB@Position - sRNA 26 -- 85@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 68 -- 74@LB@
39	0.0003198	0.02889	b3960	argH	-9.38	0.152930	179 -- 221	28 -- 64	argininosuccinate lyase		_____________178__________________________________________222\n_____________\|____________________________________________\|\n5'-GCA...UGAAU_______U_____AACAG___UAU____CUU_____________UUUAC...AUA-3'\n______________UUCAAAA_AAGGA_____AGU___GGCA___UGGGGCG_GGCGU\n______________\|\|\|\|\|\|\|_\|\|\|\|\|_____\|\|\|___\|:\|\|___\|\|\|:\|\|\|_::\|\|:\n______________AAGUUUU_UUCCU_____UCA___CUGU___ACCUCGC_UUGCG\n3'-UUU...UCAUU_____________________UU_____UAU_______G_____GGUAG...ACG-5'\n_____________\|____________________________________________\|\n_____________65___________________________________________27\n	210 -- 216	34 -- 40	-30.4591 kcal/mol	8.02766 kcal/mol	13.054 kcal/mol	178 222@LB@ \| \|@LB@5'-GCA...UGAAU U AACAG UAU CUU UUUAC...AUA-3'@LB@ UUCAAAA AAGGA AGU GGCA UGGGGCG GGCGU@LB@ \|\|\|\|\|\|\| \|\|\|\|\| \|\|\| \|:\|\| \|\|\|:\|\|\| ::\|\|:@LB@ AAGUUUU UUCCU UCA CUGU ACCUCGC UUGCG@LB@3'-UUU...UCAUU UU UAU G GGUAG...ACG-5'@LB@ \| \|@LB@ 65 27@LB@@LB@@LB@Energy -9.38 kcal/mol@LB@Hybridization Energy -30.4591 kcal/mol@LB@Unfolding Energy - mRNA 8.02766 kcal/mol@LB@Unfolding Energy - sRNA 13.054 kcal/mol@LB@@LB@Position - mRNA 179 -- 221@LB@Position - sRNA 28 -- 64@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 34 -- 40@LB@
134	0.004994	0.1313	b1518	lsrG	-14.53	0.006358	14 -- 66	30 -- 79	autoinducer-2 (AI-2) degrading protein LsrG		_____________13_______________________________________________________67\n_____________\|________________________________________________________\|\n5'-GAG...GGAAA____________GCUA____UC____CGCUUCU____UG_________________AUUUU...AAG-3'\n______________UGUGCUG_GCAG____UCGA__AGGG_______GGUG____GAUAUGGGGCGUAAU\n______________\|\|\|\|\|\|:_\|\|\|\|____\|\|:\|__\|:\|\|_______:\|\|:____:\|\|\|\|\|\|:\|\|\|:\|\|:\n______________ACACGAU_CGUC____AGUU__UUCC_______UCAU____UUAUACCUCGCGUUG\n3'-UUU...AGUUA_______A____AUUA____UU____U__________UCUG_______________CGGGU...ACG-5'\n_____________\|________________________________________________________\|\n_____________80_______________________________________________________29\n	58 -- 64	32 -- 38	-43.6874 kcal/mol	15.7058 kcal/mol	13.4555 kcal/mol	13 67@LB@ \| \|@LB@5'-GAG...GGAAA GCUA UC CGCUUCU UG AUUUU...AAG-3'@LB@ UGUGCUG GCAG UCGA AGGG GGUG GAUAUGGGGCGUAAU@LB@ \|\|\|\|\|\|: \|\|\|\| \|\|:\| \|:\|\| :\|\|: :\|\|\|\|\|\|:\|\|\|:\|\|:@LB@ ACACGAU CGUC AGUU UUCC UCAU UUAUACCUCGCGUUG@LB@3'-UUU...AGUUA A AUUA UU U UCUG CGGGU...ACG-5'@LB@ \| \|@LB@ 80 29@LB@@LB@@LB@Energy -14.53 kcal/mol@LB@Hybridization Energy -43.6874 kcal/mol@LB@Unfolding Energy - mRNA 15.7058 kcal/mol@LB@Unfolding Energy - sRNA 13.4555 kcal/mol@LB@@LB@Position - mRNA 14 -- 66@LB@Position - sRNA 30 -- 79@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 32 -- 38@LB@
153	0.00596	0.1368	b3892	fdoI	-11.89	0.036676	181 -- 200	31 -- 53	formate dehydrogenase-O cytochrome b556 subunit		_____________180_____________________201\n_____________\|_______________________\|\n5'-CCA...ACGAA___A___________________AUGAA...UGG-3'\n______________GAG_AAGACGA___GGAGCGCAA\n______________:\|\|_\|\|\|\|\|:\|___\|\|\|\|\|\|\|\|\|\n______________UUC_UUCUGUU___CCUCGCGUU\n3'-UUU...UUUCC___A_______AUA_________GCGGG...ACG-5'\n_____________\|_______________________\|\n_____________54______________________30\n	194 -- 200	31 -- 37	-24.6235 kcal/mol	2.95491 kcal/mol	9.77014 kcal/mol	180 201@LB@ \| \|@LB@5'-CCA...ACGAA A AUGAA...UGG-3'@LB@ GAG AAGACGA GGAGCGCAA@LB@ :\|\| \|\|\|\|\|:\| \|\|\|\|\|\|\|\|\|@LB@ UUC UUCUGUU CCUCGCGUU@LB@3'-UUU...UUUCC A AUA GCGGG...ACG-5'@LB@ \| \|@LB@ 54 30@LB@@LB@@LB@Energy -11.89 kcal/mol@LB@Hybridization Energy -24.6235 kcal/mol@LB@Unfolding Energy - mRNA 2.95491 kcal/mol@LB@Unfolding Energy - sRNA 9.77014 kcal/mol@LB@@LB@Position - mRNA 181 -- 200@LB@Position - sRNA 31 -- 53@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 31 -- 37@LB@
5	2.521e-06	0.001776	b3365	nirB	-15.77	0.002536	156 -- 206	35 -- 88	nitrite reductase large subunit NAD(P)H-binding		_____________155____________________________________________________207\n_____________\|______________________________________________________\|\n5'-AUC...GUUGC_______UUAA__G__G_________________A__UC_______AAAU____AAAGU...UUA-3'\n______________UGAAUCG_____G_UA_GCGGUAAU__AGAAAAG_AA__GAGGCAA____GAGC\n______________:\|\|\|\|\|:_____\|_\|\|_\|\|:\|\|\|\|\|__\|:\|\|\|\|\|_\|\|__:\|\|:\|\|\|____\|\|\|\|\n______________GCUUAGU_____C_AU_CGUCAUUA__UUUUUUC_UU__UUCUGUU____CUCG\n3'-UUU...AGCAU_______UAACA_G__A________AG_______C__CA_______AUAC____CGUUG...ACG-5'\n_____________\|______________________________________________________\|\n_____________89_____________________________________________________34\n	192 -- 198	43 -- 49	-31.7984 kcal/mol	7.74851 kcal/mol	8.27555 kcal/mol	155 207@LB@ \| \|@LB@5'-AUC...GUUGC UUAA G G A UC AAAU AAAGU...UUA-3'@LB@ UGAAUCG G UA GCGGUAAU AGAAAAG AA GAGGCAA GAGC@LB@ :\|\|\|\|\|: \| \|\| \|\|:\|\|\|\|\| \|:\|\|\|\|\| \|\| :\|\|:\|\|\| \|\|\|\|@LB@ GCUUAGU C AU CGUCAUUA UUUUUUC UU UUCUGUU CUCG@LB@3'-UUU...AGCAU UAACA G A AG C CA AUAC CGUUG...ACG-5'@LB@ \| \|@LB@ 89 34@LB@@LB@@LB@Energy -15.77 kcal/mol@LB@Hybridization Energy -31.7984 kcal/mol@LB@Unfolding Energy - mRNA 7.74851 kcal/mol@LB@Unfolding Energy - sRNA 8.27555 kcal/mol@LB@@LB@Position - mRNA 156 -- 206@LB@Position - sRNA 35 -- 88@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 43 -- 49@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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