Input and runtime details for job 1654565 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1654565 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:23:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:05 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:25:53 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:26:01 CET 2018
	Job Completed		@ Fri Feb 16 22:26:34 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1654565&toolName=CopraRNA ( 30 days expiry )

Description of the job

FnrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
150	0.005897	0.1368	b0887	cydD	-10.72	0.073556	183 -- 190	1 -- 8	glutathione/cysteine ABC transporter export permease/ATPase	_____________182______191\n_____________\|________\|\n5'-AUU...AUAAC________UAAGC...UGA-3'\n______________UCACCUGC\n______________\|\|\|\|\|\|\|\|\n______________AGUGGACG\n3'-UUU...ACGUA________-5'\n_____________\|_______\|\n_____________9_______1\n	184 -- 190	1 -- 7	-15.001 kcal/mol	1.91816 kcal/mol	2.36381 kcal/mol	182 191@LB@ \| \|@LB@5'-AUU...AUAAC UAAGC...UGA-3'@LB@ UCACCUGC@LB@ \|\|\|\|\|\|\|\|@LB@ AGUGGACG@LB@3'-UUU...ACGUA -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -10.72 kcal/mol@LB@Hybridization Energy -15.001 kcal/mol@LB@Unfolding Energy - mRNA 1.91816 kcal/mol@LB@Unfolding Energy - sRNA 2.36381 kcal/mol@LB@@LB@Position - mRNA 183 -- 190@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 1 -- 7@LB@
151	0.005924	0.1368	b3248	yhdE	-13.35	0.014399	2 -- 41	1 -- 42	dTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo-UTP pyrophosphatase	_____________1___________________________________________42\n_____________\|___________________________________________\|\n__________5'-U_____C___A_______G_____C_________U______G__GGUGG...AGC-3'\n______________UAUGG_AGC__GGCGCU_GUCGU___AUGUUGC_UUCGCU_GU\n______________\|\|\|\|\|_\|\|\|__::\|\|\|:_:\|\|\|:___\|:\|\|\|\|\|_\|\|\|:\|:_\|:\n______________AUACC_UCG__UUGCGG_UAGCG___UGCAACG_AAGUGG_CG\n3'-UUU...CUGUU_________CG______G_____AAC_______U______A__-5'\n_____________\|__________________________________________\|\n_____________43_________________________________________1\n	25 -- 31	11 -- 17	-43.9582 kcal/mol	15.3205 kcal/mol	15.293 kcal/mol	1 42@LB@ \| \|@LB@ 5'-U C A G C U G GGUGG...AGC-3'@LB@ UAUGG AGC GGCGCU GUCGU AUGUUGC UUCGCU GU@LB@ \|\|\|\|\| \|\|\| ::\|\|\|: :\|\|\|: \|:\|\|\|\|\| \|\|\|:\|: \|:@LB@ AUACC UCG UUGCGG UAGCG UGCAACG AAGUGG CG@LB@3'-UUU...CUGUU CG G AAC U A -5'@LB@ \| \|@LB@ 43 1@LB@@LB@@LB@Energy -13.35 kcal/mol@LB@Hybridization Energy -43.9582 kcal/mol@LB@Unfolding Energy - mRNA 15.3205 kcal/mol@LB@Unfolding Energy - sRNA 15.293 kcal/mol@LB@@LB@Position - mRNA 2 -- 41@LB@Position - sRNA 1 -- 42@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 11 -- 17@LB@
155	0.006038	0.137	b4374	yjjG	-10.88	0.067155	203 -- 266	1 -- 54	dUMP phosphatase	_____________202_____________________________________________________________________267\n_____________\|_______________________________________________________________________\|\n5'-CUG...AUAAU__________________UUUUCUUU______G__GAAA____GUUUACCUU____U___________G__CUGCA...CCU-3'\n______________GAAGUGGGAC____UGGA________GAUGCC_AU____CGCU_________UGAC____CAUUCACC_GC\n______________\|\|\|\|\|::\|\|\|____\|\|\|\|________:\|:\|\|\|_\|\|____\|\|\|\|_________\|\|\|\|____\|\|\|\|\|\|\|\|_\|\|\n______________CUUCAUUCUG____ACCU________UUGCGG_UA____GCGA_________ACUG____GUAAGUGG_CG\n3'-UUU...UUUUC__________UUAU____CGCG__________G_______________________CAAC________A__-5'\n_____________\|______________________________________________________________________\|\n_____________55_____________________________________________________________________1\n	257 -- 263	4 -- 10	-41.6423 kcal/mol	13.9643 kcal/mol	16.8 kcal/mol	202 267@LB@ \| \|@LB@5'-CUG...AUAAU UUUUCUUU G GAAA GUUUACCUU U G CUGCA...CCU-3'@LB@ GAAGUGGGAC UGGA GAUGCC AU CGCU UGAC CAUUCACC GC@LB@ \|\|\|\|\|::\|\|\| \|\|\|\| :\|:\|\|\| \|\| \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \|\|@LB@ CUUCAUUCUG ACCU UUGCGG UA GCGA ACUG GUAAGUGG CG@LB@3'-UUU...UUUUC UUAU CGCG G CAAC A -5'@LB@ \| \|@LB@ 55 1@LB@@LB@@LB@Energy -10.88 kcal/mol@LB@Hybridization Energy -41.6423 kcal/mol@LB@Unfolding Energy - mRNA 13.9643 kcal/mol@LB@Unfolding Energy - sRNA 16.8 kcal/mol@LB@@LB@Position - mRNA 203 -- 266@LB@Position - sRNA 1 -- 54@LB@Position Seed - mRNA 257 -- 263@LB@Position Seed - sRNA 4 -- 10@LB@
158	0.006224	0.1377	b3553	ghrB	-7.62	0.349627	252 -- 258	1 -- 7	glyoxylate/hydroxypyruvate reductase B	_____________251_____259\n_____________\|_______\|\n5'-UCA...UGCAA_______AAGAG...CAC-3'\n______________CGCCUGC\n______________\|:\|\|\|\|\|\n______________GUGGACG\n3'-UUU...CGUAA_______-5'\n_____________\|______\|\n_____________8______1\n	252 -- 258	1 -- 7	-12.8134 kcal/mol	2.86334 kcal/mol	2.34039 kcal/mol	251 259@LB@ \| \|@LB@5'-UCA...UGCAA AAGAG...CAC-3'@LB@ CGCCUGC@LB@ \|:\|\|\|\|\|@LB@ GUGGACG@LB@3'-UUU...CGUAA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -7.62 kcal/mol@LB@Hybridization Energy -12.8134 kcal/mol@LB@Unfolding Energy - mRNA 2.86334 kcal/mol@LB@Unfolding Energy - sRNA 2.34039 kcal/mol@LB@@LB@Position - mRNA 252 -- 258@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 252 -- 258@LB@Position Seed - sRNA 1 -- 7@LB@
162	0.006351	0.1377	b1869	yecN	-10.06	0.106335	266 -- 273	1 -- 8	MAPEG family inner membrane protein	_____________265______274\n_____________\|________\|\n5'-UCA...GUCGU________GAAUG...GCG-3'\n______________UCGCCUGC\n______________\|\|:\|\|\|\|\|\n______________AGUGGACG\n3'-UUU...ACGUA________-5'\n_____________\|_______\|\n_____________9_______1\n	267 -- 273	1 -- 7	-14.5941 kcal/mol	2.17055 kcal/mol	2.36381 kcal/mol	265 274@LB@ \| \|@LB@5'-UCA...GUCGU GAAUG...GCG-3'@LB@ UCGCCUGC@LB@ \|\|:\|\|\|\|\|@LB@ AGUGGACG@LB@3'-UUU...ACGUA -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -10.06 kcal/mol@LB@Hybridization Energy -14.5941 kcal/mol@LB@Unfolding Energy - mRNA 2.17055 kcal/mol@LB@Unfolding Energy - sRNA 2.36381 kcal/mol@LB@@LB@Position - mRNA 266 -- 273@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 267 -- 273@LB@Position Seed - sRNA 1 -- 7@LB@
163	0.006379	0.1377	b2210	mqo	-11.85	0.037664	161 -- 168	1 -- 8	malate dehydrogenase FAD/NAD(P)-binding domain	_____________160______169\n_____________\|________\|\n5'-GGC...AAAUC________AAUAA...AUG-3'\n______________UCACCUGC\n______________\|\|\|\|\|\|\|\|\n______________AGUGGACG\n3'-UUU...ACGUA________-5'\n_____________\|_______\|\n_____________9_______1\n	162 -- 168	1 -- 7	-15.6271 kcal/mol	1.41654 kcal/mol	2.36381 kcal/mol	160 169@LB@ \| \|@LB@5'-GGC...AAAUC AAUAA...AUG-3'@LB@ UCACCUGC@LB@ \|\|\|\|\|\|\|\|@LB@ AGUGGACG@LB@3'-UUU...ACGUA -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -11.85 kcal/mol@LB@Hybridization Energy -15.6271 kcal/mol@LB@Unfolding Energy - mRNA 1.41654 kcal/mol@LB@Unfolding Energy - sRNA 2.36381 kcal/mol@LB@@LB@Position - mRNA 161 -- 168@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 1 -- 7@LB@
167	0.006646	0.1402	b0007	yaaJ	-8.17	0.274480	254 -- 261	1 -- 8	putative transporter	_____________253______262\n_____________\|________\|\n5'-CAA...AUGAU________UCUUC...GAU-3'\n______________UUACCUGC\n______________\|:\|\|\|\|\|\|\n______________AGUGGACG\n3'-UUU...ACGUA________-5'\n_____________\|_______\|\n_____________9_______1\n	255 -- 261	1 -- 7	-12.8229 kcal/mol	2.29081 kcal/mol	2.36381 kcal/mol	253 262@LB@ \| \|@LB@5'-CAA...AUGAU UCUUC...GAU-3'@LB@ UUACCUGC@LB@ \|:\|\|\|\|\|\|@LB@ AGUGGACG@LB@3'-UUU...ACGUA -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -8.17 kcal/mol@LB@Hybridization Energy -12.8229 kcal/mol@LB@Unfolding Energy - mRNA 2.29081 kcal/mol@LB@Unfolding Energy - sRNA 2.36381 kcal/mol@LB@@LB@Position - mRNA 254 -- 261@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 1 -- 7@LB@
172	0.007014	0.1437	b3430	glgC	-13.14	0.016567	21 -- 80	1 -- 39	glucose-1-phosphate adenylyltransferase	_____________20______________________________________________________________81\n_____________\|_______________________________________________________________\|\n5'-CAG...CUCUC___U___UUUUUGAUCGCAAUU______A_________G__AACAGAGAU_____________UGGGG...UGA-3'\n______________GGA_CGC_______________AACGCC___CGCUUGA_GU_________UGU_UUUACCUGC\n______________\|\|\|_\|\|\|_______________\|\|\|\|\|\|___\|\|\|\|\|\|\|_\|\|_________\|\|:_\|\|:\|\|\|\|\|\|\n______________CCU_GCG_______________UUGCGG___GCGAACU_CA_________ACG_AAGUGGACG\n3'-UUU...UUAUA___C________________________GUA_______G______________U_________-5'\n_____________\|______________________________________________________________\|\n_____________40_____________________________________________________________1\n	74 -- 80	1 -- 7	-41.5646 kcal/mol	13.1395 kcal/mol	15.291 kcal/mol	20 81@LB@ \| \|@LB@5'-CAG...CUCUC U UUUUUGAUCGCAAUU A G AACAGAGAU UGGGG...UGA-3'@LB@ GGA CGC AACGCC CGCUUGA GU UGU UUUACCUGC@LB@ \|\|\| \|\|\| \|\|\|\|\|\| \|\|\|\|\|\|\| \|\| \|\|: \|\|:\|\|\|\|\|\|@LB@ CCU GCG UUGCGG GCGAACU CA ACG AAGUGGACG@LB@3'-UUU...UUAUA C GUA G U -5'@LB@ \| \|@LB@ 40 1@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -41.5646 kcal/mol@LB@Unfolding Energy - mRNA 13.1395 kcal/mol@LB@Unfolding Energy - sRNA 15.291 kcal/mol@LB@@LB@Position - mRNA 21 -- 80@LB@Position - sRNA 1 -- 39@LB@Position Seed - mRNA 74 -- 80@LB@Position Seed - sRNA 1 -- 7@LB@
176	0.007245	0.145	b0397	sbcC	-11.91	0.036282	117 -- 185	1 -- 55	exonuclease dsDNA ATP-dependent	_____________116____________________________________________________________________186\n_____________\|______________________________________________________________________\|\n5'-GAA...GCACU_____AACU______AUCGC___A__________CA__AUCUUUUCACCAC__________A_____C__CGGAG...UUG-3'\n______________GGAAG____GGAUGA_____AGC_GCAACGUCUG__GC_____________GACGUUGCAU_C_CCU_GC\n______________\|\|\|\|\|____:\|\|::\|_____\|\|\|_\|\|\|\|\|\|:\|::__\|\|_____________\|\|\|\|\|\|\|\|\|\|_\|_\|\|\|_\|\|\n______________CCUUC____UCUGUU_____UCG_CGUUGCGGGU__CG_____________CUGCAACGUA_G_GGA_CG\n3'-UUU...UUUUU_____AU________AUACC______________AG__AA_____________________A_U______-5'\n_____________\|_____________________________________________________________________\|\n_____________56____________________________________________________________________1\n	170 -- 176	10 -- 16	-47.2501 kcal/mol	18.5328 kcal/mol	16.8092 kcal/mol	116 186@LB@ \| \|@LB@5'-GAA...GCACU AACU AUCGC A CA AUCUUUUCACCAC A C CGGAG...UUG-3'@LB@ GGAAG GGAUGA AGC GCAACGUCUG GC GACGUUGCAU C CCU GC@LB@ \|\|\|\|\| :\|\|::\| \|\|\| \|\|\|\|\|\|:\|:: \|\| \|\|\|\|\|\|\|\|\|\| \| \|\|\| \|\|@LB@ CCUUC UCUGUU UCG CGUUGCGGGU CG CUGCAACGUA G GGA CG@LB@3'-UUU...UUUUU AU AUACC AG AA A U -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -11.91 kcal/mol@LB@Hybridization Energy -47.2501 kcal/mol@LB@Unfolding Energy - mRNA 18.5328 kcal/mol@LB@Unfolding Energy - sRNA 16.8092 kcal/mol@LB@@LB@Position - mRNA 117 -- 185@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 10 -- 16@LB@
2	4.208e-07	0.0007412	b2531	iscR	-21.46	0.000012	188 -- 264	2 -- 56	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress- and iron starvation-inducible; autorepressor	_____________187___________________________________________________________________________265\n_____________\|_____________________________________________________________________________\|\n5'-AUG...UUUGA__U___________UG_GACUGACAUCUAAA______U_____GUGACCG__A______________C__A__U___AAGCG...AAC-3'\n______________GG_GAAGUAAGACA__A______________GGGCGC_AUGCC_______CA_UGCUUGACGUUGCG_UC_AC_CUG\n______________:\|_\|\|\|\|\|\|\|\|\|\|\|__\|______________\|:\|\|\|\|_\|:\|\|\|_______\|\|_:\|\|\|\|\|\|\|\|\|\|\|\|:_\|\|_\|\|_\|\|\|\n______________UC_CUUCAUUCUGU__U______________CUCGCG_UGCGG_______GU_GCGAACUGCAACGU_AG_UG_GAC\n3'-UUU...UUUUU______________UA_AC__________________U______________A______________A_________G-5'\n_____________\|_____________________________________________________________________________\|\n_____________57____________________________________________________________________________1\n	244 -- 250	14 -- 20	-58.8762 kcal/mol	20.5805 kcal/mol	16.8438 kcal/mol	187 265@LB@ \| \|@LB@5'-AUG...UUUGA U UG GACUGACAUCUAAA U GUGACCG A C A U AAGCG...AAC-3'@LB@ GG GAAGUAAGACA A GGGCGC AUGCC CA UGCUUGACGUUGCG UC AC CUG@LB@ :\| \|\|\|\|\|\|\|\|\|\|\| \| \|:\|\|\|\| \|:\|\|\| \|\| :\|\|\|\|\|\|\|\|\|\|\|\|: \|\| \|\| \|\|\|@LB@ UC CUUCAUUCUGU U CUCGCG UGCGG GU GCGAACUGCAACGU AG UG GAC@LB@3'-UUU...UUUUU UA AC U A A G-5'@LB@ \| \|@LB@ 57 1@LB@@LB@@LB@Energy -21.46 kcal/mol@LB@Hybridization Energy -58.8762 kcal/mol@LB@Unfolding Energy - mRNA 20.5805 kcal/mol@LB@Unfolding Energy - sRNA 16.8438 kcal/mol@LB@@LB@Position - mRNA 188 -- 264@LB@Position - sRNA 2 -- 56@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 14 -- 20@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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