Input and runtime details for job 1654565 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1654565 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:23:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:05 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:25:53 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:26:01 CET 2018
	Job Completed		@ Fri Feb 16 22:26:34 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1654565&toolName=CopraRNA ( 30 days expiry )

Description of the job

FnrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
166	0.006486	0.1377	b0440	hupB	-11.63	0.043010	149 -- 200	46 -- 93	HU DNA-binding transcriptional regulator beta subunit		_____________148______________________________________________________201\n_____________\|________________________________________________________\|\n5'-CGC...CGCGU______CUUGAAG__U__________GAUAUA______AU__________AG____GUGAA...CUG-3'\n______________UCGUAC_______GA_UCAA__GUGC________AAUU__AAAGAGGAAG__AAGA\n______________\|\|\|\|\|\|_______\|\|_\|\|\|\|__\|\|\|\|________\|\|\|\|__\|\|\|:\|\|\|\|\|\|__\|\|\|\|\n______________AGCAUG_______CU_AGUU__CACG________UUAA__UUUUUCCUUC__UUCU\n3'-UUU...GCCGC_______________U____AA____AUACGUCA____GU__________A_____GUUAU...ACG-5'\n_____________\|________________________________________________________\|\n_____________94_______________________________________________________45\n	188 -- 194	51 -- 57	-29.0191 kcal/mol	11.8112 kcal/mol	5.57675 kcal/mol	148 201@LB@ \| \|@LB@5'-CGC...CGCGU CUUGAAG U GAUAUA AU AG GUGAA...CUG-3'@LB@ UCGUAC GA UCAA GUGC AAUU AAAGAGGAAG AAGA@LB@ \|\|\|\|\|\| \|\| \|\|\|\| \|\|\|\| \|\|\|\| \|\|\|:\|\|\|\|\|\| \|\|\|\|@LB@ AGCAUG CU AGUU CACG UUAA UUUUUCCUUC UUCU@LB@3'-UUU...GCCGC U AA AUACGUCA GU A GUUAU...ACG-5'@LB@ \| \|@LB@ 94 45@LB@@LB@@LB@Energy -11.63 kcal/mol@LB@Hybridization Energy -29.0191 kcal/mol@LB@Unfolding Energy - mRNA 11.8112 kcal/mol@LB@Unfolding Energy - sRNA 5.57675 kcal/mol@LB@@LB@Position - mRNA 149 -- 200@LB@Position - sRNA 46 -- 93@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 51 -- 57@LB@
173	0.007186	0.145	b3554	yiaF	-10.99	0.063148	166 -- 198	46 -- 94	barrier effect co-colonization resistance factor; DUF3053 family lipoprotein		_____________165_______________________________________________199\n_____________\|_________________________________________________\|\n5'-AAU...GUGUC________CU__UU___G_______________________________AAAUG...AGC-3'\n______________GUUGUGCG__UU__UUG_GU_______________GAAAGG_AGUAAGA\n______________\|\|:\|\|:\|\|__\|:__\|\|\|_\|:_______________:\|\|\|\|\|_\|\|\|\|\|\|\|\n______________CAGCAUGC__AG__AAC_CG_______________UUUUCC_UCAUUCU\n3'-UUU...AGCCG________UU__UU___A__AUACGUCAUUAAGUU______U_______GUUAU...ACG-5'\n_____________\|_________________________________________________\|\n_____________95________________________________________________45\n	192 -- 198	46 -- 52	-24.2986 kcal/mol	7.73667 kcal/mol	5.5768 kcal/mol	165 199@LB@ \| \|@LB@5'-AAU...GUGUC CU UU G AAAUG...AGC-3'@LB@ GUUGUGCG UU UUG GU GAAAGG AGUAAGA@LB@ \|\|:\|\|:\|\| \|: \|\|\| \|: :\|\|\|\|\| \|\|\|\|\|\|\|@LB@ CAGCAUGC AG AAC CG UUUUCC UCAUUCU@LB@3'-UUU...AGCCG UU UU A AUACGUCAUUAAGUU U GUUAU...ACG-5'@LB@ \| \|@LB@ 95 45@LB@@LB@@LB@Energy -10.99 kcal/mol@LB@Hybridization Energy -24.2986 kcal/mol@LB@Unfolding Energy - mRNA 7.73667 kcal/mol@LB@Unfolding Energy - sRNA 5.5768 kcal/mol@LB@@LB@Position - mRNA 166 -- 198@LB@Position - sRNA 46 -- 94@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 46 -- 52@LB@
188	0.008258	0.1545	b3803	hemX	-8.31	0.258101	280 -- 297	46 -- 62	putative uroporphyrinogen III methyltransferase		_____________279________________298\n_____________\|__________________\|\n5'-AAA...CGCAA_______AAA________ACA-3'\n______________CAGAAAA___GAGUAAGA\n______________\|\|:\|\|\|\|___:\|\|\|\|\|\|\|\n______________GUUUUUU___UUCAUUCU\n3'-UUU...AUUAA_______CC_________GUUAU...ACG-5'\n_____________\|__________________\|\n_____________63_________________45\n	291 -- 297	46 -- 52	-11.8819 kcal/mol	0.215347 kcal/mol	3.36135 kcal/mol	279 298@LB@ \| \|@LB@5'-AAA...CGCAA AAA ACA-3'@LB@ CAGAAAA GAGUAAGA@LB@ \|\|:\|\|\|\| :\|\|\|\|\|\|\|@LB@ GUUUUUU UUCAUUCU@LB@3'-UUU...AUUAA CC GUUAU...ACG-5'@LB@ \| \|@LB@ 63 45@LB@@LB@@LB@Energy -8.31 kcal/mol@LB@Hybridization Energy -11.8819 kcal/mol@LB@Unfolding Energy - mRNA 0.215347 kcal/mol@LB@Unfolding Energy - sRNA 3.36135 kcal/mol@LB@@LB@Position - mRNA 280 -- 297@LB@Position - sRNA 46 -- 62@LB@Position Seed - mRNA 291 -- 297@LB@Position Seed - sRNA 46 -- 52@LB@
130	0.00465	0.126	b2916	argP	-9.43	0.148863	238 -- 266	47 -- 73	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor		_____________237___________________________267\n_____________\|_____________________________\|\n5'-GGC...ACUGG__________ACG____C_____UU____CGCGC...CAG-3'\n______________AUGCGGUGAU___UGAA_GAGGA__UGAG\n______________\|\|\|\|:\|\|:\|\|___::\|\|_:\|\|\|\|__\|:\|\|\n______________UACGUCAUUA___GUUU_UUCCU__AUUC\n3'-UUU...CACGA__________A______U_____UC____UGUUA...ACG-5'\n_____________\|_____________________________\|\n_____________74____________________________46\n	240 -- 246	65 -- 71	-19.9139 kcal/mol	5.97985 kcal/mol	4.50495 kcal/mol	237 267@LB@ \| \|@LB@5'-GGC...ACUGG ACG C UU CGCGC...CAG-3'@LB@ AUGCGGUGAU UGAA GAGGA UGAG@LB@ \|\|\|\|:\|\|:\|\| ::\|\| :\|\|\|\| \|:\|\|@LB@ UACGUCAUUA GUUU UUCCU AUUC@LB@3'-UUU...CACGA A U UC UGUUA...ACG-5'@LB@ \| \|@LB@ 74 46@LB@@LB@@LB@Energy -9.43 kcal/mol@LB@Hybridization Energy -19.9139 kcal/mol@LB@Unfolding Energy - mRNA 5.97985 kcal/mol@LB@Unfolding Energy - sRNA 4.50495 kcal/mol@LB@@LB@Position - mRNA 238 -- 266@LB@Position - sRNA 47 -- 73@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 65 -- 71@LB@
142	0.005444	0.1351	b3950	frwB	-10.99	0.063119	13 -- 50	47 -- 86	putative enzyme IIB component of PTS	b3899	_____________12__________________________________________51\n_____________\|___________________________________________\|\n5'-GCA...GGGGU____U__________C__________________C___C____UUAUA...GGG-3'\n______________GGUC_GAUUGUGCUG__CGGUGGUU____GAAGG_AAG_UGGG\n______________::\|\|_:\|\|\|\|\|\|\|\|:__\|:\|\|::\|\|____:\|\|\|\|_\|\|\|_\|::\|\n______________UUAG_UUAACACGAU__GUCAUUAA____UUUCC_UUC_AUUC\n3'-UUU...CAUGC_______________AC________GUUU______________UGUUA...ACG-5'\n_____________\|___________________________________________\|\n_____________87__________________________________________46\n	30 -- 36	63 -- 69	-27.1662 kcal/mol	11.4404 kcal/mol	4.74035 kcal/mol	12 51@LB@ \| \|@LB@5'-GCA...GGGGU U C C C UUAUA...GGG-3'@LB@ GGUC GAUUGUGCUG CGGUGGUU GAAGG AAG UGGG@LB@ ::\|\| :\|\|\|\|\|\|\|\|: \|:\|\|::\|\| :\|\|\|\| \|\|\| \|::\|@LB@ UUAG UUAACACGAU GUCAUUAA UUUCC UUC AUUC@LB@3'-UUU...CAUGC AC GUUU UGUUA...ACG-5'@LB@ \| \|@LB@ 87 46@LB@@LB@@LB@Energy -10.99 kcal/mol@LB@Hybridization Energy -27.1662 kcal/mol@LB@Unfolding Energy - mRNA 11.4404 kcal/mol@LB@Unfolding Energy - sRNA 4.74035 kcal/mol@LB@@LB@Position - mRNA 13 -- 50@LB@Position - sRNA 47 -- 86@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 63 -- 69@LB@
9	1.504e-05	0.005627	b1189	dadA	-11.44	0.048458	195 -- 226	48 -- 78	D-amino acid dehydrogenase		_____________194_______________________________227\n_____________\|_________________________________\|\n5'-CCG...UCGGA__G_______G___UG____UACU_________UGUGG...CGG-3'\n______________GU_GCUAUGC_AGU___UCA____GGGAAGUGG\n______________\|\|_\|\|\|\|\|\|\|_\|\|\|___\|\|\|____:\|\|\|\|\|\|::\n______________CA_CGAUACG_UCA___AGU____UCCUUCAUU\n3'-UUU...GUUAA______________UUA___UUUU_________CUGUU...ACG-5'\n_____________\|_________________________________\|\n_____________79________________________________47\n	220 -- 226	48 -- 54	-26.7579 kcal/mol	10.7545 kcal/mol	4.57065 kcal/mol	194 227@LB@ \| \|@LB@5'-CCG...UCGGA G G UG UACU UGUGG...CGG-3'@LB@ GU GCUAUGC AGU UCA GGGAAGUGG@LB@ \|\| \|\|\|\|\|\|\| \|\|\| \|\|\| :\|\|\|\|\|\|::@LB@ CA CGAUACG UCA AGU UCCUUCAUU@LB@3'-UUU...GUUAA UUA UUUU CUGUU...ACG-5'@LB@ \| \|@LB@ 79 47@LB@@LB@@LB@Energy -11.44 kcal/mol@LB@Hybridization Energy -26.7579 kcal/mol@LB@Unfolding Energy - mRNA 10.7545 kcal/mol@LB@Unfolding Energy - sRNA 4.57065 kcal/mol@LB@@LB@Position - mRNA 195 -- 226@LB@Position - sRNA 48 -- 78@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 48 -- 54@LB@
49	0.0005476	0.03937	b2432	yfeY	-8.84	0.200409	189 -- 199	48 -- 58	RpoE-regulated lipoprotein		_____________188_________200\n_____________\|___________\|\n5'-GCU...UAACC___________CAUGA...ACU-3'\n______________AAAGGAAGUGA\n______________\|\|\|\|\|\|\|\|\|:\|\n______________UUUCCUUCAUU\n3'-UUU...AGUUU___________CUGUU...ACG-5'\n_____________\|___________\|\n_____________59__________47\n	193 -- 199	48 -- 54	-12.8959 kcal/mol	3.31176 kcal/mol	0.745549 kcal/mol	188 200@LB@ \| \|@LB@5'-GCU...UAACC CAUGA...ACU-3'@LB@ AAAGGAAGUGA@LB@ \|\|\|\|\|\|\|\|\|:\|@LB@ UUUCCUUCAUU@LB@3'-UUU...AGUUU CUGUU...ACG-5'@LB@ \| \|@LB@ 59 47@LB@@LB@@LB@Energy -8.84 kcal/mol@LB@Hybridization Energy -12.8959 kcal/mol@LB@Unfolding Energy - mRNA 3.31176 kcal/mol@LB@Unfolding Energy - sRNA 0.745549 kcal/mol@LB@@LB@Position - mRNA 189 -- 199@LB@Position - sRNA 48 -- 58@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 48 -- 54@LB@
76	0.001842	0.08273	b1717	rpmI	-9.61	0.135308	218 -- 256	48 -- 92	50S ribosomal subunit protein L35		_____________217___________________________________________257\n_____________\|_____________________________________________\|\n5'-GCG...AAGAC_____________GC_____A___C____________ACC_____AGGUG...UGA-3'\n______________CGUACG___CGGU__UGCUA_GCG____UUCAAAAAA___GGUAA\n______________\|\|\|\|\|\|___\|::\|__\|\|\|\|\|_\|\|:____\|\|\|\|\|\|\|\|\|___:\|\|\|\|\n______________GCAUGC___GUUA__ACGAU_CGU____AAGUUUUUU___UCAUU\n3'-UUU...CCGCA______UUA____AC_____A___CAUU_________CCU_____CUGUU...ACG-5'\n_____________\|_____________________________________________\|\n_____________93____________________________________________47\n	242 -- 248	56 -- 62	-26.2826 kcal/mol	11.8203 kcal/mol	4.85131 kcal/mol	217 257@LB@ \| \|@LB@5'-GCG...AAGAC GC A C ACC AGGUG...UGA-3'@LB@ CGUACG CGGU UGCUA GCG UUCAAAAAA GGUAA@LB@ \|\|\|\|\|\| \|::\| \|\|\|\|\| \|\|: \|\|\|\|\|\|\|\|\| :\|\|\|\|@LB@ GCAUGC GUUA ACGAU CGU AAGUUUUUU UCAUU@LB@3'-UUU...CCGCA UUA AC A CAUU CCU CUGUU...ACG-5'@LB@ \| \|@LB@ 93 47@LB@@LB@@LB@Energy -9.61 kcal/mol@LB@Hybridization Energy -26.2826 kcal/mol@LB@Unfolding Energy - mRNA 11.8203 kcal/mol@LB@Unfolding Energy - sRNA 4.85131 kcal/mol@LB@@LB@Position - mRNA 218 -- 256@LB@Position - sRNA 48 -- 92@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 56 -- 62@LB@
110	0.003505	0.1114	b4111	proP	-11.30	0.052648	186 -- 222	48 -- 93	proline/glycine betaine transporter		_____________185________________________________________________223\n_____________\|__________________________________________________\|\n5'-CAC...CCAAC_________GA_____________U________________AAAA_____AACCG...GUA-3'\n______________UC_UGCGAG__AA____GCUAUGC_________GAAAAGGA____AGUAA\n______________\|\|_\|:\|\|\|:__\|\|____\|\|\|\|\|\|\|_________:\|\|\|\|\|\|\|____\|\|\|\|\|\n______________AG_AUGCUU__UU____CGAUACG_________UUUUUCCU____UCAUU\n3'-UUU...GCCGC__C______AG__AACA_______UCAUUAAGU_________________CUGUU...ACG-5'\n_____________\|__________________________________________________\|\n_____________94_________________________________________________47\n	198 -- 204	70 -- 76	-22.6159 kcal/mol	6.45165 kcal/mol	4.87071 kcal/mol	185 223@LB@ \| \|@LB@5'-CAC...CCAAC GA U AAAA AACCG...GUA-3'@LB@ UC UGCGAG AA GCUAUGC GAAAAGGA AGUAA@LB@ \|\| \|:\|\|\|: \|\| \|\|\|\|\|\|\| :\|\|\|\|\|\|\| \|\|\|\|\|@LB@ AG AUGCUU UU CGAUACG UUUUUCCU UCAUU@LB@3'-UUU...GCCGC C AG AACA UCAUUAAGU CUGUU...ACG-5'@LB@ \| \|@LB@ 94 47@LB@@LB@@LB@Energy -11.30 kcal/mol@LB@Hybridization Energy -22.6159 kcal/mol@LB@Unfolding Energy - mRNA 6.45165 kcal/mol@LB@Unfolding Energy - sRNA 4.87071 kcal/mol@LB@@LB@Position - mRNA 186 -- 222@LB@Position - sRNA 48 -- 93@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 70 -- 76@LB@
194	0.008684	0.1548	b1242	ychE	-7.20	0.413978	105 -- 113	48 -- 56	UPF0056 family inner membrane protein		_____________104_______114\n_____________\|_________\|\n5'-CAU...UAGUU_________AAAAU...GCA-3'\n______________AGGAGGUGA\n______________\|\|\|\|:\|\|:\|\n______________UCCUUCAUU\n3'-UUU...UUUUU_________CUGUU...ACG-5'\n_____________\|_________\|\n_____________57________47\n	107 -- 113	48 -- 54	-10.6218 kcal/mol	2.70901 kcal/mol	0.718267 kcal/mol	104 114@LB@ \| \|@LB@5'-CAU...UAGUU AAAAU...GCA-3'@LB@ AGGAGGUGA@LB@ \|\|\|\|:\|\|:\|@LB@ UCCUUCAUU@LB@3'-UUU...UUUUU CUGUU...ACG-5'@LB@ \| \|@LB@ 57 47@LB@@LB@@LB@Energy -7.20 kcal/mol@LB@Hybridization Energy -10.6218 kcal/mol@LB@Unfolding Energy - mRNA 2.70901 kcal/mol@LB@Unfolding Energy - sRNA 0.718267 kcal/mol@LB@@LB@Position - mRNA 105 -- 113@LB@Position - sRNA 48 -- 56@LB@Position Seed - mRNA 107 -- 113@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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