Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
149	0.06369	0.3424	ct_124	accC	-5.62	0.277702	171 -- 193	17 -- 31	acetyl-CoA carboxylase biotin carboxylase subunit	_____________170_____________________194\n_____________\|_______________________\|\n5'-CUC...GUUCU____________AUAGUUAA___UUAGU...UGG-3'\n______________AAGUUAUUCCGU________GGC\n______________\|\|\|:\|\|\|\|\|\|:\|________\|\|\|\n______________UUCGAUAAGGUA________CCG\n3'-UUU...CAGUC_______________________CAAUC...GAA-5'\n_____________\|_______________________\|\n_____________32______________________16\n	176 -- 182	20 -- 26	-15.1557 kcal/mol	4.67447 kcal/mol	4.86101 kcal/mol	170 194@LB@ \| \|@LB@5'-CUC...GUUCU AUAGUUAA UUAGU...UGG-3'@LB@ AAGUUAUUCCGU GGC@LB@ \|\|\|:\|\|\|\|\|\|:\| \|\|\|@LB@ UUCGAUAAGGUA CCG@LB@3'-UUU...CAGUC CAAUC...GAA-5'@LB@ \| \|@LB@ 32 16@LB@@LB@@LB@Energy -5.62 kcal/mol@LB@Hybridization Energy -15.1557 kcal/mol@LB@Unfolding Energy - mRNA 4.67447 kcal/mol@LB@Unfolding Energy - sRNA 4.86101 kcal/mol@LB@@LB@Position - mRNA 171 -- 193@LB@Position - sRNA 17 -- 31@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 20 -- 26@LB@
192	0.08479	0.363	ct_293	accD	-5.01	0.360945	270 -- 276	79 -- 85	acetyl-CoA carboxylase carboxyltransferase subunit beta	_____________269_____277\n_____________\|_______\|\n5'-GUA...GUGGC_______AAUGU...UGA-3'\n______________UGGCUAA\n______________:\|\|\|\|\|\|\n______________GCCGAUU\n3'-UUU...UCUAU_______GAGGU...GAA-5'\n_____________\|_______\|\n_____________86______78\n	270 -- 276	79 -- 85	-7.87376 kcal/mol	1.76808 kcal/mol	1.09751 kcal/mol	269 277@LB@ \| \|@LB@5'-GUA...GUGGC AAUGU...UGA-3'@LB@ UGGCUAA@LB@ :\|\|\|\|\|\|@LB@ GCCGAUU@LB@3'-UUU...UCUAU GAGGU...GAA-5'@LB@ \| \|@LB@ 86 78@LB@@LB@@LB@Energy -5.01 kcal/mol@LB@Hybridization Energy -7.87376 kcal/mol@LB@Unfolding Energy - mRNA 1.76808 kcal/mol@LB@Unfolding Energy - sRNA 1.09751 kcal/mol@LB@@LB@Position - mRNA 270 -- 276@LB@Position - sRNA 79 -- 85@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 79 -- 85@LB@
152	0.064	0.3424	ct_749	alaS	-4.53	0.436714	274 -- 290	41 -- 57	alanine--tRNA ligase	_____________273_______________291\n_____________\|_________________\|\n5'-AAA...AUCUU_______UUUCCC____AAUGA...CUU-3'\n______________CUCCUGU______GCAU\n______________\|:\|\|\|\|\|______:\|\|:\n______________GGGGACA______UGUG\n3'-UUU...GAAGG_______CAUGUU____AUCUC...GAA-5'\n_____________\|_________________\|\n_____________58________________40\n	274 -- 280	51 -- 57	-13.0187 kcal/mol	0.670655 kcal/mol	7.82241 kcal/mol	273 291@LB@ \| \|@LB@5'-AAA...AUCUU UUUCCC AAUGA...CUU-3'@LB@ CUCCUGU GCAU@LB@ \|:\|\|\|\|\| :\|\|:@LB@ GGGGACA UGUG@LB@3'-UUU...GAAGG CAUGUU AUCUC...GAA-5'@LB@ \| \|@LB@ 58 40@LB@@LB@@LB@Energy -4.53 kcal/mol@LB@Hybridization Energy -13.0187 kcal/mol@LB@Unfolding Energy - mRNA 0.670655 kcal/mol@LB@Unfolding Energy - sRNA 7.82241 kcal/mol@LB@@LB@Position - mRNA 274 -- 290@LB@Position - sRNA 41 -- 57@LB@Position Seed - mRNA 274 -- 280@LB@Position Seed - sRNA 51 -- 57@LB@
68	0.02545	0.29	ct_369	aroB	-8.93	0.051509	264 -- 292	1 -- 31	3-dehydroquinate synthase	_____________263_____________________________293\n_____________\|_______________________________\|\n5'-GGC...ACCCU_________GCUU_______UC_________CCCUCUCA-3'\n______________AAGCUGUUU_____CGUU_G__UACCGACUU\n______________\|\|\|\|\|:\|\|:_____\|\|\|\|_\|__\|\|\|\|:\|\|\|\|\n______________UUCGAUAAG_____GCAA_C__AUGGUUGAA\n3'-UUU...CAGUC_________GUACC____U_UU_________-5'\n_____________\|______________________________\|\n_____________32_____________________________1\n	286 -- 292	1 -- 7	-23.4181 kcal/mol	6.00993 kcal/mol	8.47514 kcal/mol	263 293@LB@ \| \|@LB@5'-GGC...ACCCU GCUU UC CCCUCUCA-3'@LB@ AAGCUGUUU CGUU G UACCGACUU@LB@ \|\|\|\|\|:\|\|: \|\|\|\| \| \|\|\|\|:\|\|\|\|@LB@ UUCGAUAAG GCAA C AUGGUUGAA@LB@3'-UUU...CAGUC GUACC U UU -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -8.93 kcal/mol@LB@Hybridization Energy -23.4181 kcal/mol@LB@Unfolding Energy - mRNA 6.00993 kcal/mol@LB@Unfolding Energy - sRNA 8.47514 kcal/mol@LB@@LB@Position - mRNA 264 -- 292@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 1 -- 7@LB@
103	0.03832	0.3036	ct_382	aroG	-9.59	0.035559	138 -- 150	48 -- 60	3-deoxy-7-phosphoheptulonate synthase	_____________137___________151\n_____________\|_____________\|\n5'-UUU...UUUUC_____________UAUGA...CUA-3'\n______________UCUUCCCUGUGUG\n______________\|\|::\|\|\|\|\|\|\|\|:\n______________AGGGGGGACACAU\n3'-UUU...ACUGA_____________GUUUG...GAA-5'\n_____________\|_____________\|\n_____________61____________47\n	144 -- 150	48 -- 54	-20.2814 kcal/mol	1.44568 kcal/mol	9.24642 kcal/mol	137 151@LB@ \| \|@LB@5'-UUU...UUUUC UAUGA...CUA-3'@LB@ UCUUCCCUGUGUG@LB@ \|\|::\|\|\|\|\|\|\|\|:@LB@ AGGGGGGACACAU@LB@3'-UUU...ACUGA GUUUG...GAA-5'@LB@ \| \|@LB@ 61 47@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -20.2814 kcal/mol@LB@Unfolding Energy - mRNA 1.44568 kcal/mol@LB@Unfolding Energy - sRNA 9.24642 kcal/mol@LB@@LB@Position - mRNA 138 -- 150@LB@Position - sRNA 48 -- 60@LB@Position Seed - mRNA 144 -- 150@LB@Position Seed - sRNA 48 -- 54@LB@
10	0.001873	0.154	ct_367	aroL	-15.55	0.000817	132 -- 208	3 -- 59	shikimate kinase	_____________131_______________________________________________________________________________209\n_____________\|_________________________________________________________________________________\|\n5'-UGU...ACAAA_______CUGUAACACACA___________________UUCCA__________AUUGCA___C____CACUAC________GUUCG...UGC-3'\n______________CCCUCCU____________GUGUAUAAAUA__AGAGUU______UAUUUCGUG______GCG__AGA______AUGCCAAC\n______________\|\|\|:\|\|\|____________\|\|\|\|\|:\|\|\|:\|__\|\|\|\|\|:______\|\|\|\|:\|:\|\|______\|\|\|__\|\|\|______\|\|:\|\|\|\|\|\n______________GGGGGGA____________CACAUGUUUGU__UCUCAG______AUAAGGUAC______CGC__UCU______UAUGGUUG\n3'-UUU...CUGAA______________________________GA______UCUUCG__________________AA_________________AA-5'\n_____________\|_________________________________________________________________________________\|\n_____________60________________________________________________________________________________2\n	202 -- 208	3 -- 9	-46.252 kcal/mol	13.1534 kcal/mol	17.5467 kcal/mol	131 209@LB@ \| \|@LB@5'-UGU...ACAAA CUGUAACACACA UUCCA AUUGCA C CACUAC GUUCG...UGC-3'@LB@ CCCUCCU GUGUAUAAAUA AGAGUU UAUUUCGUG GCG AGA AUGCCAAC@LB@ \|\|\|:\|\|\| \|\|\|\|\|:\|\|\|:\| \|\|\|\|\|: \|\|\|\|:\|:\|\| \|\|\| \|\|\| \|\|:\|\|\|\|\|@LB@ GGGGGGA CACAUGUUUGU UCUCAG AUAAGGUAC CGC UCU UAUGGUUG@LB@3'-UUU...CUGAA GA UCUUCG AA AA-5'@LB@ \| \|@LB@ 60 2@LB@@LB@@LB@Energy -15.55 kcal/mol@LB@Hybridization Energy -46.252 kcal/mol@LB@Unfolding Energy - mRNA 13.1534 kcal/mol@LB@Unfolding Energy - sRNA 17.5467 kcal/mol@LB@@LB@Position - mRNA 132 -- 208@LB@Position - sRNA 3 -- 59@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 3 -- 9@LB@
70	0.02698	0.29	ct_381	artJ	-6.76	0.162330	168 -- 194	31 -- 57	arginine ABC transporter substrate-binding protein ArtJ	_____________167__________________________195\n_____________\|____________________________\|\n5'-AUU...UAUAG______CC__UA__C_____________GUGAG...GUG-3'\n______________UCCCUG__GU__GA_CAUU_GAGUCGGA\n______________:\|\|\|\|\|__\|\|__:\|_\|\|:\|_\|\|\|\|\|:\|\|\n______________GGGGAC__CA__UU_GUGA_CUCAGUCU\n3'-UUU...GAAGG______A___UG__U____U________UCGAU...GAA-5'\n_____________\|____________________________\|\n_____________58___________________________30\n	188 -- 194	31 -- 37	-25.975 kcal/mol	9.18513 kcal/mol	10.0297 kcal/mol	167 195@LB@ \| \|@LB@5'-AUU...UAUAG CC UA C GUGAG...GUG-3'@LB@ UCCCUG GU GA CAUU GAGUCGGA@LB@ :\|\|\|\|\| \|\| :\| \|\|:\| \|\|\|\|\|:\|\|@LB@ GGGGAC CA UU GUGA CUCAGUCU@LB@3'-UUU...GAAGG A UG U U UCGAU...GAA-5'@LB@ \| \|@LB@ 58 30@LB@@LB@@LB@Energy -6.76 kcal/mol@LB@Hybridization Energy -25.975 kcal/mol@LB@Unfolding Energy - mRNA 9.18513 kcal/mol@LB@Unfolding Energy - sRNA 10.0297 kcal/mol@LB@@LB@Position - mRNA 168 -- 194@LB@Position - sRNA 31 -- 57@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 31 -- 37@LB@
121	0.04894	0.3262	ct_310	atpE	-7.64	0.103947	27 -- 34	80 -- 87	V-type ATP synthase subunit E	_____________26_______35\n_____________\|________\|\n5'-GCA...CCUUC________CCUUC...UUC-3'\n______________UACGGCUG\n______________\|\|\|\|\|\|\|:\n______________AUGCCGAU\n3'-UUU...CCUCU________UGAGG...GAA-5'\n_____________\|________\|\n_____________88_______79\n	28 -- 34	80 -- 86	-11.9035 kcal/mol	3.17039 kcal/mol	1.09752 kcal/mol	26 35@LB@ \| \|@LB@5'-GCA...CCUUC CCUUC...UUC-3'@LB@ UACGGCUG@LB@ \|\|\|\|\|\|\|:@LB@ AUGCCGAU@LB@3'-UUU...CCUCU UGAGG...GAA-5'@LB@ \| \|@LB@ 88 79@LB@@LB@@LB@Energy -7.64 kcal/mol@LB@Hybridization Energy -11.9035 kcal/mol@LB@Unfolding Energy - mRNA 3.17039 kcal/mol@LB@Unfolding Energy - sRNA 1.09752 kcal/mol@LB@@LB@Position - mRNA 27 -- 34@LB@Position - sRNA 80 -- 87@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 80 -- 86@LB@
174	0.07335	0.3448	ct_777	bioF	-7.25	0.126962	76 -- 92	19 -- 34	8-amino-7-oxononanoate synthase	_____________75________________93\n_____________\|_________________\|\n5'-CUG...UCAGG_______G_____U___UUGGG...UUU-3'\n______________UAGAAGC_AUUCC_AUG\n______________:\|\|\|\|\|\|_\|\|\|\|\|_\|\|\|\n______________GUCUUCG_UAAGG_UAC\n3'-UUU...UCUCA_______A_________CGCAA...GAA-5'\n_____________\|_________________\|\n_____________35________________18\n	76 -- 82	28 -- 34	-16.4612 kcal/mol	4.23516 kcal/mol	4.97732 kcal/mol	75 93@LB@ \| \|@LB@5'-CUG...UCAGG G U UUGGG...UUU-3'@LB@ UAGAAGC AUUCC AUG@LB@ :\|\|\|\|\|\| \|\|\|\|\| \|\|\|@LB@ GUCUUCG UAAGG UAC@LB@3'-UUU...UCUCA A CGCAA...GAA-5'@LB@ \| \|@LB@ 35 18@LB@@LB@@LB@Energy -7.25 kcal/mol@LB@Hybridization Energy -16.4612 kcal/mol@LB@Unfolding Energy - mRNA 4.23516 kcal/mol@LB@Unfolding Energy - sRNA 4.97732 kcal/mol@LB@@LB@Position - mRNA 76 -- 92@LB@Position - sRNA 19 -- 34@LB@Position Seed - mRNA 76 -- 82@LB@Position Seed - sRNA 28 -- 34@LB@
127	0.0509	0.3262	ct_808	cafE	-7.80	0.095102	169 -- 204	18 -- 57	ribonuclease E	_____________168_________________________________________205\n_____________\|___________________________________________\|\n5'-GUU...GAGGA_______A______________CAU______ACG_________AAAAC...AAA-3'\n______________CUCCUGU____GAGCGC_AGAG___UUAGGA______CCAUGG\n______________\|:\|\|\|\|\|____:\|:\|:\|_\|\|\|\|___\|:\|\|:\|______\|\|\|\|\|\|\n______________GGGGACA____UUUGUG_UCUC___AGUCUU______GGUACC\n3'-UUU...GAAGG_______CAUG______A_____________CGAUAA______GCAAU...GAA-5'\n_____________\|___________________________________________\|\n_____________58__________________________________________17\n	169 -- 175	51 -- 57	-30.1775 kcal/mol	9.43695 kcal/mol	12.9362 kcal/mol	168 205@LB@ \| \|@LB@5'-GUU...GAGGA A CAU ACG AAAAC...AAA-3'@LB@ CUCCUGU GAGCGC AGAG UUAGGA CCAUGG@LB@ \|:\|\|\|\|\| :\|:\|:\| \|\|\|\| \|:\|\|:\| \|\|\|\|\|\|@LB@ GGGGACA UUUGUG UCUC AGUCUU GGUACC@LB@3'-UUU...GAAGG CAUG A CGAUAA GCAAU...GAA-5'@LB@ \| \|@LB@ 58 17@LB@@LB@@LB@Energy -7.80 kcal/mol@LB@Hybridization Energy -30.1775 kcal/mol@LB@Unfolding Energy - mRNA 9.43695 kcal/mol@LB@Unfolding Energy - sRNA 12.9362 kcal/mol@LB@@LB@Position - mRNA 169 -- 204@LB@Position - sRNA 18 -- 57@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 51 -- 57@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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