| 45 |
0.002015 |
0.1494 |
bsu34040 |
yvfW |
-14.27 |
0.041807 |
34 -- 68 |
10 -- 49 |
lactate utilization protein B |
|
_____________33_______________________________________69\n_____________|________________________________________|\n5'-GCG...AAAAA___GA___G_________AAAC__________________GAUCG...GUC-3'\n______________GUC___AU_UG_UGGAGG_____GGGAGCAGAG_GU_GCU\n______________:||___||_||_|:||||_____||||:|||||_||_|||\n______________UAG___UA_AC_AUCUCC_____CCCUUGUCUC_CA_CGA\n3'-UUU...AAGCC___AAC__G__U______CCAAC__________U__U___AGAGA...AUA-5'\n_____________|________________________________________|\n_____________50_______________________________________9\n |
55 -- 61 |
19 -- 25 |
-34.7578 kcal/mol |
10.5343 kcal/mol |
9.9558 kcal/mol |
33 69@LB@ | |@LB@5'-GCG...AAAAA GA G AAAC GAUCG...GUC-3'@LB@ GUC AU UG UGGAGG GGGAGCAGAG GU GCU@LB@ :|| || || |:|||| ||||:||||| || |||@LB@ UAG UA AC AUCUCC CCCUUGUCUC CA CGA@LB@3'-UUU...AAGCC AAC G U CCAAC U U AGAGA...AUA-5'@LB@ | |@LB@ 50 9@LB@@LB@@LB@Energy -14.27 kcal/mol@LB@Hybridization Energy -34.7578 kcal/mol@LB@Unfolding Energy - mRNA 10.5343 kcal/mol@LB@Unfolding Energy - sRNA 9.9558 kcal/mol@LB@@LB@Position - mRNA 34 -- 68@LB@Position - sRNA 10 -- 49@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 19 -- 25@LB@ |
| 93 |
0.007837 |
0.2778 |
bsu33430 |
yvgQ |
-5.79 |
0.632053 |
172 -- 180 |
38 -- 46 |
sulfite reductase [NADPH] hemoprotein beta-component |
|
_____________171_______181\n_____________|_________|\n5'-GCU...GUAUA_________CUUGA...UGA-3'\n______________UUGAUUUGA\n______________|||||:|||\n______________AACUAGACU\n3'-UUU...CCUAG_________AUCUC...AUA-5'\n_____________|_________|\n_____________47________37\n |
174 -- 180 |
38 -- 44 |
-9.05403 kcal/mol |
2.31378 kcal/mol |
0.946105 kcal/mol |
171 181@LB@ | |@LB@5'-GCU...GUAUA CUUGA...UGA-3'@LB@ UUGAUUUGA@LB@ |||||:|||@LB@ AACUAGACU@LB@3'-UUU...CCUAG AUCUC...AUA-5'@LB@ | |@LB@ 47 37@LB@@LB@@LB@Energy -5.79 kcal/mol@LB@Hybridization Energy -9.05403 kcal/mol@LB@Unfolding Energy - mRNA 2.31378 kcal/mol@LB@Unfolding Energy - sRNA 0.946105 kcal/mol@LB@@LB@Position - mRNA 172 -- 180@LB@Position - sRNA 38 -- 46@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 132 |
0.01159 |
0.2883 |
bsu33140 |
yvqJ |
-14.19 |
0.043338 |
185 -- 204 |
31 -- 52 |
MFS transporter |
|
_____________184____________________205\n_____________|______________________|\n5'-GAC...GGCAA____GG___C_________U__UGAAU...UUA-3'\n______________CGGG____G_UCUGAUGGA_GG\n______________|||:____|_||||||:||_||\n______________GCCU____C_AGACUAUCU_CC\n3'-UUU...AAAAA____AGAA_U_________C__CAACC...AUA-5'\n_____________|______________________|\n_____________53_____________________30\n |
195 -- 201 |
34 -- 40 |
-19.4408 kcal/mol |
4.27934 kcal/mol |
0.976414 kcal/mol |
184 205@LB@ | |@LB@5'-GAC...GGCAA GG C U UGAAU...UUA-3'@LB@ CGGG G UCUGAUGGA GG@LB@ |||: | ||||||:|| ||@LB@ GCCU C AGACUAUCU CC@LB@3'-UUU...AAAAA AGAA U C CAACC...AUA-5'@LB@ | |@LB@ 53 30@LB@@LB@@LB@Energy -14.19 kcal/mol@LB@Hybridization Energy -19.4408 kcal/mol@LB@Unfolding Energy - mRNA 4.27934 kcal/mol@LB@Unfolding Energy - sRNA 0.976414 kcal/mol@LB@@LB@Position - mRNA 185 -- 204@LB@Position - sRNA 31 -- 52@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 54 |
0.002747 |
0.1697 |
bsu35790 |
yvyI |
-14.15 |
0.043919 |
186 -- 197 |
25 -- 36 |
mannose-6-phosphate isomerase YvyI |
|
_____________185__________198\n_____________|____________|\n5'-UCU...ACAGG________AC__AUCAU...AAU-3'\n______________GGAGGGGU__GG\n______________:|||||||__||\n______________UCUCCCCA__CC\n3'-UUU...GACUA________AC__CUUGU...AUA-5'\n_____________|____________|\n_____________37___________24\n |
187 -- 193 |
29 -- 35 |
-17.2174 kcal/mol |
2.92719 kcal/mol |
0.135926 kcal/mol |
185 198@LB@ | |@LB@5'-UCU...ACAGG AC AUCAU...AAU-3'@LB@ GGAGGGGU GG@LB@ :||||||| ||@LB@ UCUCCCCA CC@LB@3'-UUU...GACUA AC CUUGU...AUA-5'@LB@ | |@LB@ 37 24@LB@@LB@@LB@Energy -14.15 kcal/mol@LB@Hybridization Energy -17.2174 kcal/mol@LB@Unfolding Energy - mRNA 2.92719 kcal/mol@LB@Unfolding Energy - sRNA 0.135926 kcal/mol@LB@@LB@Position - mRNA 186 -- 197@LB@Position - sRNA 25 -- 36@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 29 -- 35@LB@ |
| 136 |
0.01205 |
0.2883 |
bsu35319 |
yvzG |
-14.21 |
0.042782 |
258 -- 272 |
27 -- 43 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________257_______________273\n_____________|_________________|\n5'-AAG...UUACC_________________UGUUA...AUU-3'\n______________AUC_GA_AGAGGGGUUG\n______________|||_||_||||||||||\n______________UAG_CU_UCUCCCCAAC\n3'-UUU...AGAAC___A__A__________CCCUU...AUA-5'\n_____________|_________________|\n_____________44________________26\n |
266 -- 272 |
27 -- 33 |
-20.1473 kcal/mol |
4.96591 kcal/mol |
0.966729 kcal/mol |
257 273@LB@ | |@LB@5'-AAG...UUACC UGUUA...AUU-3'@LB@ AUC GA AGAGGGGUUG@LB@ ||| || ||||||||||@LB@ UAG CU UCUCCCCAAC@LB@3'-UUU...AGAAC A A CCCUU...AUA-5'@LB@ | |@LB@ 44 26@LB@@LB@@LB@Energy -14.21 kcal/mol@LB@Hybridization Energy -20.1473 kcal/mol@LB@Unfolding Energy - mRNA 4.96591 kcal/mol@LB@Unfolding Energy - sRNA 0.966729 kcal/mol@LB@@LB@Position - mRNA 258 -- 272@LB@Position - sRNA 27 -- 43@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 27 -- 33@LB@ |
| 142 |
0.01236 |
0.2883 |
bsu37940 |
ywdJ |
-14.78 |
0.033255 |
157 -- 193 |
27 -- 52 |
purine permease YwdJ |
|
_____________156___________________________________194\n_____________|_____________________________________|\n5'-GGA...GGAAA____GAG____U_______UUUUAAAG____A_____AGCUU...ACU-3'\n______________CGGA___UCUU_AUUUGAU________AGAG_GGUUG\n______________||||___||||_||:||||________||||_|||||\n______________GCCU___AGAA_UAGACUA________UCUC_CCAAC\n3'-UUU...AAAAA___________C___________________C_____CCCUU...AUA-5'\n_____________|_____________________________________|\n_____________53____________________________________26\n |
169 -- 175 |
37 -- 43 |
-23.5373 kcal/mol |
7.77274 kcal/mol |
0.980744 kcal/mol |
156 194@LB@ | |@LB@5'-GGA...GGAAA GAG U UUUUAAAG A AGCUU...ACU-3'@LB@ CGGA UCUU AUUUGAU AGAG GGUUG@LB@ |||| |||| ||:|||| |||| |||||@LB@ GCCU AGAA UAGACUA UCUC CCAAC@LB@3'-UUU...AAAAA C C CCCUU...AUA-5'@LB@ | |@LB@ 53 26@LB@@LB@@LB@Energy -14.78 kcal/mol@LB@Hybridization Energy -23.5373 kcal/mol@LB@Unfolding Energy - mRNA 7.77274 kcal/mol@LB@Unfolding Energy - sRNA 0.980744 kcal/mol@LB@@LB@Position - mRNA 157 -- 193@LB@Position - sRNA 27 -- 52@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 140 |
0.01224 |
0.2883 |
bsu36480 |
ywoD |
-6.33 |
0.569704 |
31 -- 37 |
66 -- 72 |
MFS transporter |
|
_____________30______38\n_____________|_______|\n5'-AUU...AUAUC_______AAAGC...AUA-3'\n______________GGGGUGG\n______________|||||:|\n______________CCCCAUC\n3'-UUU...ACCGC_______GCGGU...AUA-5'\n_____________|_______|\n_____________73______65\n |
31 -- 37 |
66 -- 72 |
-11.1749 kcal/mol |
4.83937 kcal/mol |
0.00366414 kcal/mol |
30 38@LB@ | |@LB@5'-AUU...AUAUC AAAGC...AUA-3'@LB@ GGGGUGG@LB@ |||||:|@LB@ CCCCAUC@LB@3'-UUU...ACCGC GCGGU...AUA-5'@LB@ | |@LB@ 73 65@LB@@LB@@LB@Energy -6.33 kcal/mol@LB@Hybridization Energy -11.1749 kcal/mol@LB@Unfolding Energy - mRNA 4.83937 kcal/mol@LB@Unfolding Energy - sRNA 0.00366414 kcal/mol@LB@@LB@Position - mRNA 31 -- 37@LB@Position - sRNA 66 -- 72@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 66 -- 72@LB@ |
| 129 |
0.01144 |
0.2883 |
bsu36230 |
ywqF |
-11.45 |
0.129133 |
185 -- 195 |
31 -- 41 |
UDP-glucose 6-dehydrogenase YwqF |
|
_____________184_________196\n_____________|___________|\n5'-UUU...UUUAA___U_______AAACA...CUC-3'\n______________CUG_UGGAGGG\n______________|||_|:|||||\n______________GAC_AUCUCCC\n3'-UUU...AACUA___U_______CAACC...AUA-5'\n_____________|___________|\n_____________42__________30\n |
189 -- 195 |
31 -- 37 |
-16.042 kcal/mol |
3.66073 kcal/mol |
0.928126 kcal/mol |
184 196@LB@ | |@LB@5'-UUU...UUUAA U AAACA...CUC-3'@LB@ CUG UGGAGGG@LB@ ||| |:|||||@LB@ GAC AUCUCCC@LB@3'-UUU...AACUA U CAACC...AUA-5'@LB@ | |@LB@ 42 30@LB@@LB@@LB@Energy -11.45 kcal/mol@LB@Hybridization Energy -16.042 kcal/mol@LB@Unfolding Energy - mRNA 3.66073 kcal/mol@LB@Unfolding Energy - sRNA 0.928126 kcal/mol@LB@@LB@Position - mRNA 185 -- 195@LB@Position - sRNA 31 -- 41@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 90 |
0.006982 |
0.2588 |
bsu40920 |
yyaF |
-12.87 |
0.074972 |
6 -- 31 |
25 -- 50 |
GTP-dependent nucleic acid-binding protein EngD |
|
_____________5___________________________32\n_____________|___________________________|\n______5'-CAAAU___ACAAAU_______AU__AA_____AAGUA...CGU-3'\n______________GAU______UCUGAUA__GG___UGGG\n______________|||______|||||||__||___||||\n______________CUA______AGACUAU__CC___ACCC\n3'-UUU...AAAGC___GAACU________CU__CCA____CUUGU...AUA-5'\n_____________|___________________________|\n_____________51__________________________24\n |
15 -- 21 |
36 -- 42 |
-17.5884 kcal/mol |
3.73957 kcal/mol |
0.976057 kcal/mol |
5 32@LB@ | |@LB@ 5'-CAAAU ACAAAU AU AA AAGUA...CGU-3'@LB@ GAU UCUGAUA GG UGGG@LB@ ||| ||||||| || ||||@LB@ CUA AGACUAU CC ACCC@LB@3'-UUU...AAAGC GAACU CU CCA CUUGU...AUA-5'@LB@ | |@LB@ 51 24@LB@@LB@@LB@Energy -12.87 kcal/mol@LB@Hybridization Energy -17.5884 kcal/mol@LB@Unfolding Energy - mRNA 3.73957 kcal/mol@LB@Unfolding Energy - sRNA 0.976057 kcal/mol@LB@@LB@Position - mRNA 6 -- 31@LB@Position - sRNA 25 -- 50@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 55 |
0.002845 |
0.1725 |
bsu40380 |
yycI |
-20.10 |
0.001970 |
172 -- 209 |
12 -- 51 |
two-component system YycFG regulatory protein |
|
_____________171_________________________________________210\n_____________|___________________________________________|\n5'-CAG...ACAAA_____UA____G____A_______GCU________U_______AAUAA...GCA-3'\n______________GGAUU__UUGA___GA_GGAGGGG______AACAG_GGAGUGG\n______________||||:__||||___||_:||||||______|||||_:||||:|\n______________CCUAG__AACU___CU_UCUCCCC______UUGUC_UCUCAUC\n3'-UUU...AAAAG___________AGA__A_______AACCCC_____________GAAGA...AUA-5'\n_____________|___________________________________________|\n_____________52__________________________________________11\n |
203 -- 209 |
12 -- 18 |
-35.1528 kcal/mol |
5.09356 kcal/mol |
9.95619 kcal/mol |
171 210@LB@ | |@LB@5'-CAG...ACAAA UA G A GCU U AAUAA...GCA-3'@LB@ GGAUU UUGA GA GGAGGGG AACAG GGAGUGG@LB@ ||||: |||| || :|||||| ||||| :||||:|@LB@ CCUAG AACU CU UCUCCCC UUGUC UCUCAUC@LB@3'-UUU...AAAAG AGA A AACCCC GAAGA...AUA-5'@LB@ | |@LB@ 52 11@LB@@LB@@LB@Energy -20.10 kcal/mol@LB@Hybridization Energy -35.1528 kcal/mol@LB@Unfolding Energy - mRNA 5.09356 kcal/mol@LB@Unfolding Energy - sRNA 9.95619 kcal/mol@LB@@LB@Position - mRNA 172 -- 209@LB@Position - sRNA 12 -- 51@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 12 -- 18@LB@ |
